CS271100B1 - Biotechnical method of invertase production - Google Patents

Abstract

Yeast cells in suspension are continuously broken up in a spindle mill by micro-glass spheroids, the invertase- containing solution thereby obtained is deslurryed to provide a clear fiitrate by use of a sheet filter, whereafter the clear solution is passed to a combined ultra- and microfiltration plant in such a manner that the solution first runs through an ultrafiltration separation unit, and thereafter is passed over a microfiltration unit. The invertase concentrate solution so obtained is stabilised, e.g. by the addition of glycerol, standardised and packed in known manner. <IMAGE>

Description

271 100

Ha3BaHHe Η3ΟβρβΤβΗΗΗ

BHOTEXHHHECKHH METOfl ΠΡΟΗ3ΠΟΛΟΤΌΛ HI1BEPTA3U □ eJiacTb npKMeneiiHH ΗΟΟβρβτβΗΗΗ H3O <speTeHHe xacaeTCx «HOTexHHHecxoro mgtoaa προηοβοαοτββ ΦβρΜβΗτβ Ηΐιββρτβ3ΐ4 to βχτηβημχxjieeHux apowweň, HcnoJibayeMoro npeHMyiuecTBeHHo b nHuienotí npoMuuuieHHacTH.

XapaxTepMCTHKa h3bbcthnx tbxhhhbckhx peMeiinK

Hsbbctho ηηογο mbtoaob προη3βοα <? Τββ ΗΗΒβρτβ3Μ H3 apomokbH. B OCHOBHOM 3TH MBTOAU XapaKTepH3yBTCH CJieaynUtHHH PaeOMHMH onepailHBMH: - Pa3pyuieHHe apomokbbux kjibtok ajih ocBoeomaeiiHn HHBepTa3u

- OTABJIBIIHB ΗΗΒβρΤβ3Μ OT COCTaBHMX HacTeH KJIBTOK - KoHueHTpauHfi hHHCTKa pacTBOpa. coaepwauiero HHBepTa3y

- CTaeHJIH3aUHX HHBBPTB3M

Msbbctbh, HanpHMep, cnocoe pa3pyuieiiHii kjibtok πρμ πομοιιιη opraiiHHecKHX pacTBopxTejieň.HanpHMep, TOJiyeHa. Πο οκοηηβηηη njia3McuiH3a / aBTOJiH3a προηοχοαητ OTaejieHwe nepacTBopeHHuxapomokbbux κΑβτοκ npx πομοιιιη aHJibTpnpecca. nocjie cooTBeTCTByeuiero ycTaiiOBJieHHH 3naieHHSi pilaBTOJiH3BTa προηοχοαητ neuiyMBHHe nnBepTa3M nocpeacTBOM ποβτορηο προβολημογο ocaMcneiiHSiaueTOHOM. flocjie peaJiH3aiiHH Apyrnx HeoexoAHMUx onepaunA well οταβλβηη »h pacTBopeHHio noJiyiemiuHpacTBop HHBepTa3M ocBoeowAaeTCfl οτ οοτβτκοβ pacTBopHTeJin πρη πομοιιιη BaxyyM-BwnapHoro annapaTaΗ AOBOAHTCH ΛΟ ΟΠΡβΛβΛΒΗΗΜΧ ΚΟΗΚβΙΙΤΡβΠΗΗ. 3aT6M ΠρΗ ΠΟΜΟΙΙΙΗ rJIHlXBpOJia ΠΡΟΗ3ΒΟΛΗΤΟΗCTaeHJiHaauHH (BajibauíMHAT - ΛβΗτιι. Ε. Η Bajuic. Λ.Κ. βιιηγθ πο χημηη ΠΗίαβ npoayKToe τ.11 / 2. Λ3Λ. ΙΙΙπρΗΗΓθρΦΒρΛβΓ. BepJiHH. 1933 r. CTp. 734-738).

HeaocTaTKH οτογο μβτοαβ npewAe ecero β τομ. ίτο Ίβοτο οοτβτκη χαοβητογο TojiyeHa βpacTBope HHBepTaau οοτβητοπ and npoayiiTe. paspyuieHHe kjibtok προτθκλθτ Aojiro, mto noBuuiaeTOnaCHOCTb pa3MHON <eHHH eaKTepaB. and AOCTHrHyTue BUPaeOTKH H aKTHBHOCTb ηβ3ηβίητβαζ> ημ · πρηίθμpaCXOA ΟΗβρΓΗΗ CpaBHHTBJIbHO «OJIbUloH.

AonoJiHHTejibHaH oepaeoTKa aBTOJiHaaTa. nojiyMeHHoro πρτβμ npHeaejieHHH TOJiyeHa. nanaHHOM, aTaxwe ejiaroaapn apvthm μβροπρηητηημ. xax, HanpHMep, aacopeuHH γηαροοκηοη bjiiomhhhh hjih φοοφθτβKBJlbUHH, 3jnOHpOBaHHK> paCTBOpOM JlaKTBTa, BOAoH HJIH paCTBOpOM caxaposM. OCaWABHHlO ΟΠΗΡΤΟΜ HJIHpacTBopoM nHKpaTa, ληβλη3ΟΜ η προίημ onepauHHM no omhctkb, Ηβ πρηβοαητ to cyuiecTBeHHOMynoBbiuieHHn 3 «* bkthbhocth MBToaa CXapTMafiep, Β.Γοραηβη. 78, 320-324 (1978)).

ApyroH H3BecTiiM {<mbtoa onHcaH b DD-PS 1401. 3βτβΜ cjieayeT κοηπβητρβηηη nyTencenapauHH c nocJiBAynuieff AoeaBKoK ocBoeoiKAaiouiHX bbuibctb, τβΚΗΧ, HanpHMep. xax 3ejieHuň cojioahjih ero aHacTaTHHBCKHe eHOJiorHHecxH axTHBHMe BemecTBa. b t.h. UHTaaa.

Pa3pyuieHHe KJieTox προηοχοαητ πόα BO3AeftcTBHeM yjit »Tpa3Byxa Ha BpauianuiyiocH Apown <eByioMaccy. Ilocjie ocBoeowABHHH cocTaBHux nacTeH λροηοκθΑ πρη πομοιιιη cenapauHH προηοχοαητ οτΑ &amp; OnHmenHeKJIBTOMHoS IKHAKOCTH ΠΡΟΗΟΧΟΑΗΤ ΠΡΗ ΠΟΜΟΙΙΙΗ CJlaeOKOCJIOÍÍ ΟβΜβΗΗοΚ CMOJ1U. 271 100 2

HeflocTaTOK 3Toro MeToaa npewAe Bcero b tom. hto BucoKHe TexHanorHMecKH-TexiiHMecKHeH3Aepx <KH m CBasaHHaa c sthm μοκηαη TexHojiorna. bmcokhH pacxoa βηθργηη οτηοοητθαβηο bmcokhHpacxoA epeMeHH Ha ocBoeomAeHHe cocTaBHux nacTeR ΑροΗοκβΗ, caa3aHHoe b He3HaHHTejibHMM BuxoaoH, T.e .. flocTHraeMoň βκτοβιιοοτβιο nojiyxeHHoon ΗΗΒβρτβ3Μ. aejianT btot cnocoe HepeHTaeejibHMM.

AaJiee H3bbctbh μθτολ nojiyneiiHa pacTBopHM HHBepTa3W H3 αροχοκθΗ, κβκ onncaHO b DE-PS 2626 626. 3τοτ motoa no cyitiecTBy oTJiHsaeTca τβΜ. hto ejiaroAapa BO3AeňcTBHw uejunojias, προτβθ3 hjiHna3 Ha apoxoKeBbie κλθτκη προηοχοαητ nJia3MOJtH3 η ββταπΗ3. B KanecTee η3χοαημχ αροχοκθΗHcnojibsynTcn apomokh, eucyuieHHue μθτολομ pacnuAeiiHa. κοτορμθ 3βτβΜ ποβτορηο cycneHAHpynTca.nocjie ycneiuHoro njia3MajiH3a / aBTOjiH3a προηοχολητ OTaejienHe HepacTBopHMux ΦρβΓΜβΗτοβ κπθτοκ hαβτθΜ «HJibTpaT nocpeACTBOM yjibTpa3Byxa pa3aeAaioT Ha apoxoxeeoň βκστρβκτ H HHBepTaay. 3 3 3 T a a a a a a CTaenjiH3anHa hjih cTaHAapTH3auHa miBepTa3U ποορθαοτβομ pacTBopa raiHuepona hjihCOPÓHTa. ΠOM 3TOM ΜβΤΟΑβ TBKWe BHAHO. HTO paCXOA ΒΗβΡΓΗΗ CpaBHHTejIbHO BUCOK. ΒρβΜΠpacTBopetiHH nonpextHBMy bmcoko (8-20 h). hto MoxteT πρηβθοτη κ npopaiuHBatiHn η Ten csmum knoTepan aKTHBiiocTH. KpoMe Toro, NPH Hcnojib3OBatiHH BucyuieiniNx rivet pacnuJieHHa ApoxoxeH hcBX3aHHoro c sthm co6jiwa © hhh bhcokoH TeMnepaTypu (and 240 ° C> HMeerca onaCHocTb napunajibHoRHHaKTHBaUHH «epMBHTOB. B DD-PS 110 425 H 116 135 onncuBaioTca Μβτοωι naJiyneHHa apoxmeBux SKCTpaKTOB. ApomxeBoro TJIHKaHa. ΑρΟΗΟΚΘΒΗΧ ΠΡΟΤΒΗΗΟΒΜΧ H3OJ1HTOB C ΠΟΙΙΗΙΚβΗΗΜΜ ΟΟΑθρΗΟΗΗβΜ HyKJieHHOBOň KHCJIOTM. Λροχοκθβμθ kjistkh (h πηβημθ xjieeHue apomokh) nocjie βτογο pa3Apoejia »TCN η3Βθοτημμ cnocoeoM.ΗβΠΡΗΜβΡ. ΓΟΜΟΓΘΗΗ3ΗρθΒβΗΗΘΜ ΠΟΑ AaBJleHHeM. ΡβΟΤΗΡβΗΗβΜ Β ΠβΟΟΗΗΟΗ HJIH KOJIJIOHAHOR MejlbHHUe.Ρ83ΜβΑΒΗΘΗΗΘΜ ΠρΗ ΠΟΜΟΙΙΙΗ 3ByKOBMX ΒΟΑΙΙ," ΑβΓΟΑβΡΗ ΠΟΒΤΟΡΗΜΜ UHKJ1BM 3βΜθρβ) ΚΗΒβΗΗΗ ΗΟΤΤβΗΒβΗΗΗ. ηρΗ ΠΟΜΟΙΙΙΗ ΑΗΤΗΗβΟΚΗΧ ΦβΡΜβΗΤΟΒ. Π Πρ. 3βΤβΜ CJleAyeT ΤβΠΑΟΒΒΗ ΟβρββΟΤΚΘ C UeAblDΟΤΑβΛΘΗΗΗ HyKABHHOBoff KHCJIOTM ΡΟΤΘΗΗ ΠΡΟΤΘΗΗβ. ΠΟΟΑΘ 3ΤΟΓΟ CABAyeT Ηθ OnHCaHHOB OJ e ie ie e e e ep OJ ep ep ep ep ep ep ep ep ep ep ep ep ep ep ep ο KT KT B B nojiyneHHe αροχοκθβογο προτβΗΗβ c ποηηικθηημμCOAeptXaHHBM HyKJieHHOBoR KHCAOTU npOHCXOAHT ΠΡΗ ΠΟΜΟΙΙΙΗ UeilTPH »yrHPOBaHHa. BaKyyMHoRKOHUBHTpaUHH H CyilIKH. 3th mbtoam b nepeyu οηβρθαβ cjiywaT aah nojiyneHHa h3oahtob αρομοκθβμχ προτθηηοβ cΠΟΗΗΜβΗΗΗΗ ΟΟΑβΡΜβΗΗΘΜ HyKAGHHOBOR KHCAOTU. ApOIKWeBHX 3ΑΗΚβΗΟΒ H 3KCTpBKTOB- χθχηοαογημθοκηθ onepauHH no cyuiecTBy ποχοικη on τοχηολογημβοκηθ onepauHH, npHMeHHMue πρηnOJiyHBHHH HHBBpTaSM. ΟΑΗβΚΟ B Η3Ο6ΡβΤΘΗΗΗ Η a AaHO ΗΗΚβΚΗΧ yKa3aHHfl ΠΟ nepeHOCHMOCTHΠΡΘΑΑΟΗΙΘΗΗΟΓΟ cnOCOBa Ha nOJiyHGHHB ΗΗΒβρΤβ3Μ. KββΗΗΟ npeAJlOHteHHMtt KBK npeHMyiUeCTBBHHMR ΜβΤΟΑ ΓΟΑΟΓβΗΗ3βΗΗΗ ΠΟΑ BMCOKHM ΑβΒΑβΗΗβΜ AAHpa3pyUieHHSt ΚΑβΤΟΚ Η B 3 * «eKTHBHOCTHnOJiyHOHHH ΗΗΒβρΤβ3Μ TaKHM cnocoeoM. B DE-PS 26 39 129 onHCueanTca μθτοαμ οταθαβημη ΦβρΜβΗΤΟΒ. rae ΦβΡΜβΗΤΜ pacTBOpanTca

HanpHMep. nyTeM paapyiueHHH καθτοκ πηβημχ ApoxoKeH h / hah οβρββοτκοΒ ABTepreHTaMH η ΒΜβοτβ cοοτβτκβΜΗ pa3pyuieHHMx KjieTOK hjih KJieTxaMH pacnpeAeJiínoTca Meway pa3jiHHHtiMH «a3aMH boabhhctoRμηογο · β3οβοΗ chctbmm. coAepwaiiieB ηηηημϋμ oaho BHcoKOMOJieicyjisipHoe coeAHHeHHe H3 rpynn .. With WOBaiIHMX paCTBOpeHHMMH npH HeoeXOAHMOCTH noJIHMepHMMH cnHpTaMH. nOJItO * HpaMH. CAOIKHMMHnOJ) H 3 «HpaMH. nOAHBHHHAnHPOJIHAOHaMH H nOJlHCaXaPHAaMH, H MHHHMyM OAHy HeOpraHHMeCKy »COJIb. HAHwe coAepwauieR abb BMCOKOMOJieKyjiapHue coeAHHeHHsi. nocjie nero «a3M oTAejiaioTcsi oAh οτ ApyroHΠΡΗ ΠΟΜΟΙΙΙΗ, HanpHMep. OCaWAeHHH. yAbTpa * HAbTpailHH. AHaAH3a. MeTaKOAAOHAHOrO npOHHUallHH.aACOpeUHH, ΗΟΗΟΟβΜΘΗβ HAH 3ΑβΚΤρθ * Ορβ3Β. Π ΠρβΑΛΟΙΚβΗΗΟΜ ΟΠΟΟΟββ rÓBOpHTCSI O TOM. HTO MOHtHO nOAyHHTb ΑΜβΜβ ΦβρΜβΗΤΜ. ΚΟΚΚΡβΤΗΟ.ΟΑΗβκο, roeopHTca TOJibKO on nyjinJiaiiaaax h MajibTaeax. Pe3yjihTaTU, noJiynaeMue πρη πομοιιιηnpeAJÍOWeHHOrO cnocoea. Ry MOryT. OAHaKO. yAOBYouTBOpHTb B OTHOUleHHH aKTHBHOCTH H BMXOAa. eCJIHCOnOCTABHTb C ΗβΟβΧΟΑΗΜΜΜΗ AJla 3ΤΟΓΟ H3Aep) MKaMH. 271 100

Uejib H3oepeTeiinn

UeJlb OCβρβΤβΗΗΗ COCTOHT B TOM. MTO "SN pa3Pa0OTaTb 3KOXOMRIUHH 3ΗβΡΓΗΒ βΗΟΤβΧΗΗΗβΟΚΧίίμθτολ Ajir noJiyxeiiHR BucoKoaKTHBHoR η οβθρχμηοτοΚ HHeepTa3U H3 xjieeHUx apoHUKeň, kotophH nenOJIbSOBaJICR EU OpraHHHeCKHMH paCTBOPHTeJIRMH HJIH ApyrHMH XHMHKaTaMH H / HJIH ΦΘΡΜβΗΤβΜΗ. H raeΒρβΜΗ npOHSBOACTBa Sb SbijiO <SH KpaTKHM. AaJlee MeTOA AQJHMeH «SbITb JlerKO peaJIH3yeMUM H HecjiomHMM.ejiaroaapsi xcnojib2OBaxHB cooTBBTCTBynuiero necJiowHoro oeopyAOBaHHR. a paeoTa AOJiiKHa npoeoAHTbCRHenpepuBHo, see oAHOBpeMeiiHO peajiH3OBaTbCH bucokhH bmxoa κοηχθητρηροββηχοΗHHBepTa3bi. and οτχοαη η ποβοΜΗΗβ npoAyxTH aojiwhu eMTb πρΗΓΟΑΚΗ κ AaJTbHefiuieň aepaeoTxe.

Nsjíoweime cyamocTM χβοβρβτβΗΗκ

ApowuKeBbie KJieTKH axTHBHoH cycneHCHH xjieaiiux Apowwefi c 10 -MM cyxoro BemecTBa <HTS), hTeMnepaTypoft ot 8 - 15 ° C cTepwHeeoB μθαβηηκθ. πρηηθμΑΗβΜβτρ HcnoJibayeMux MHKpocTexJioiuapHKOB - ot 0.5 - 1.2 mm. and mhcjio οβοροτοΒ pasMeuiHBamuero

BaJia - 2,500 - 4,000 οβ. / MHH. B nocjieAoeaTejibHo npHCoeAxxeHHOM cenapaTope xenpeptiBHo npoxcxoAHT rpyeoe OTAejiexHe paapyuieMHbix kjibtok ot coaepiKautero HHBepTaay pacTBopa. MtosunpeaoTBpaTHTb ποτθρη φθρμθητοβ πρη βμχοαβ, nepea oceeTJieHHeM πρητοκ cycneiWHxocTaHaBAHBaeTcx, η ροτορ okojio 5 MHHyT npoMueaeTCR boaoH. πρΗΗβΜ eMKOCTb ajir ocBeTJieHHRtojibko TorAa onopowHsieTcx. xoraa y cjihbb γοτοβογο npoAyxTa cenapaTOpa bmxoaht mmctar Baaa.

Cjieaynutasi οβτβΜ HenpepHBHaa * HJibTpauHR ajir οοββταβιιηχ coAepwaiuero HHBepTaay pacTBopanpOHCXOAHT ΠΡΗ flOMOUIH CJlOHCTOrO «HJIbTpa. ΠΡΗΜβΜ npOAyCKHBH CnOCOeHOCTb CJ1O6B «HJIbTpa - 425 -475 JI / Mac. M2. OcBeTJIRBUlHe CJIOH «HJIbTpa ΠΡΗ OTOK ΑΟββΒΟΜΗΟ npOKJlaAMBaBTCR« HJlbTPOBajlbHofteyMarofl, ηρΗΜβΜ caoh «tuibTpa nepea HaMaAOM« HAbTpaitHH okojio 20 μηη. προμμβββτοη boaoH.Pa3HHUa AaBJíeHHR MBWAy BXOAOM H BHXOAOM «HAbTPA BO ΒρβΜΗ HA HAbTpaitHH He AOJDKHa eMTb BMIU6 200 -250 KPa. CAOHCTmR «HAbTP ΠΟΑΒΚΑΒΜ6Η TaKHM O« 5pa3OM. MTO HaPRAV C npOlteCCOM «HAbTpaitHH BO3MOMHaUHpxyARUHSi. Tax mto npeAOTBpauiaeTCH oapa3OBaHxe Η3 «μτομηογο αββαβηηη. Moryuiero npxeecTH κnopxe nJiacTHH «tuibTpa. OctsbiiihRcr nocjie uxpKyjisiUHH octbtok οοβθταχβιιιθγο pacTBopa HHBepTaoubhob noABepraeTcn cenapauHH.

HxcTKa. KOHueHTpauHsi x CTepHAHsattHR pacTBopa HHBepTaaM προηοχοαητ npx noMOtuxKOMOHHHPOBaiiHoR ycTaxoBKH yjibTpa- x mhkpo- «HAbTpaitHH. Λ3βΛ paaaeAeHHR «HAbTpauHeR TaxpacnojioweH κ y3jiy ρβ3ΑθΑβΗΗΗ MHxpo« HAbTpauHeH. mto npemae ecero pacTBop OMHiuaeTCR hKOHueHTpxpyeTCR b yejie yjibTpa «HJibTpauHH. 3βτβΜ πβπρβΒΑΗβτοχ κ CTepHjiH3auxH Mepe3 y3eaMHKpQ «HAbTpailHH. nponycKiiax cnocoaxocTb HcnoAbayeMHx b pasAejinnuieM y3jie yjibTpa * HjibTpauxx MexepaH UFcocTasJinT. 80-140 ji / Mac.M2. ρχ 0,4ο - - - - - - - - - - - ok ok ok ok ok5555555555555555555555555555 - - - - - - - - - - - - - - - - - - - 0.1 - 0.3 KPa. Sar ποβμιιιβηηχ MexaitHMeCKoR CTaexJibHOCTH παοοκηχ ΜβΜβρβκ MemayMeMepaHaMH x nJtacTHHaMH «tuibTpa noMeutaeTCR onopHaa TKaHb. HanpHMep. ηθτκαηημΗ μβτθρηβα.

PacTBop HHBepTa3M crymaeTcn b yaji yjibTpa «xjibTpauxH npH6AH3HTeAbHO Ha 1/10. AjirAajibHeButeB omxctkh KoxuexTpaTa pacTBopa ηηβθρτβομ προχοχοαητ AoeaeoMHaR AHa «xxbTpaitHH apa3AeARBUieM y3jie yjibTpa« xjibTpauHH. KoxuexTpaT npx stom pa3eaejiReTCR boaoH b cooTHouieHxx 1 1 x and τβχ nop Axa «xjibTpyeTCR uxpKyAxpoBaxHeM. noxa onRTb AOCTxrxyT hcxoakuH oeteM KoxuexTpaTa.

FlocJie 3toH yjibTpa- x Axa * HjibTpauxK OMXuieHHuft pacTBop xotmeHTpaTa KHBepTaabi noAaeTCR ajirOTAejieHHR coAepwauxxcR b pacTBopa AeHaTypxpoBaxHux ~ POTexxoB x MXKpoopraxxaMOB and pa3AejiH »uixSy3eJi Mxxpo * HJibTpauHH. AHaMBTp nop xcnojib3yeMoro MaTepxajia Mxxpo * HjibTpa cocTaBJíRBT 0.2 mm.ΠΡΗΜβΜ ΒΧΟΑΗΟβ ΑβΒυίθΗΗβ Ηβ AOJIWXO ΠΡβΒΜΙΙΙβΤΒ 0.3 MPa. &amp; ΠΗ nOBUUieXHR MeXaXHMeCXOS CTaeXJIbHOCTHπρημβηηβμογο «HAbTpauHOHHoro MaTepwajia Hcnojib3yeTCR, xax onHcaxo b Aeae paoAeJiRBiuero y3JiayjibTpa * HJibTpaiiHH. onopHan TxaHb. nocpeACTBOM nocjieAyBuieň x SHaxoMoR cTaeHAHaauHH pacTBop aoboáhtcr αο βκτηβηοοτο okojio 1500 U X MJI-1 Η AO CnOCOBHOCTH Κ ΧΡβΗβΗΗΒ.

OTββΗΗΟ ΠρΗΓΟΑβΗ COOTBeTCTByBlUHfi Η3ΟβΡβΤβΗΗΒ ΜβΤΟΑ AJIR nOJiyMBHHR ΠρβΠβρβΤΟΒ HHBePTa3U bhcokoR cneiiH * KMecKott axTHBHOCTH (400 - 800 oti. / Mhh.). OTOTuepepepepepejíjíjíjíjíjí BBBHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTIRIRIRIRIRIRIRIR IRΗIRIRIRIRIRIRIRIRIR HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HAHTHTHTHTHTHTHTHTHTHTHTHTHTHTHTHT HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HAHTHTHTHTHTHTHTHTHT HA HA HAHTHTHT HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA HA 271 99 4 nojiytaeMuB corJiacHo οπηορηηομι / MeTOAy bmxoa cTa «sHJiH3HPOBanHoro rjiHuepojioM pacTsopaΗΗΒβρτβ3Η cocTaBJíneT 75 * b nepecteTe Ha Hcnojii> 3yeMue aKTHBiiue xjietsmie apoxokh.

ΠρΗΜβρ ocytqecTBJíeiiHn HsoepeTeiiHH cxbmm noacHneTca HHmecJteayiouiHfi npHMep ocyuiecTBJieHHa H3oepeTeHHH. 120 κγ xpaHHBuiHXca npH TexnepaType 2 ° C aKTHBHUx xjteeHbix ApommeH (HTS 30 -33 *)

HarpeBaeTca b am am θ ο ix ix ix ix ix m m m m m m m m m m m m m m m TS TS TS TS TS TS TS TS TS TS TS TS TS TS TS TS TS TS TS.

OxjiawfleHHaíi cycneHCHa 3βτβΜ smitest ACiaaTop 2 nocTynaeT b nenpepiiBiio oepaeoTaaeuiy »cTepmHeeyn MeJtbiiHuy third PaapyuieHHe ΛΡΟχοκβΒΝκ κσιετοκ πρη θτομ προηοχοαητ oxjiawaeHHOM boaoB.npHdJIH3HT8JIt b> HO-3 AHTpOBOM MeJlbHHHHOM ΓΟΡΙΙΙΚβ, AO BUCOTH B 0.8 - 1 MM HaBajla npH6JIH3HTejtbHO 3000 οβ. / MHH .. and nponycKHaa cnocoeHocTb cycnen3HH - 16 Ji / tac. ΠρΗΒβΑβΗΗβΗ b yaoeHyn jute nepepaeoTKH «opxy apommeBan cycneH3Ha smitest βθρχηηΒ bmxoa μθαβηοηηογο ropuixa nocTynaeT beMKOCTb Ajia pa3MeuiHBaiiHH η HarpeeaHHH fourth tepe3 Αθ3βτορ 5 cycneH3HH bboahtch b cenapaTop 6.3flecb eoJibiuaa tacTb octbtkob paapyuieHHUx kjibtok οτΑβΛΗβτοπ οτ pacTBopa ΗΗββρτβ3Μ. H3 cjiHBacenapaTopa αππ γοτοβογο npooyxTa 6 βμχολητ * A3a, πομτη οοβοθμ ocBoeoMtAeHHaa οτ οοτβτκοβpa3pyuieHHMX κΛβτοκ h nocTynaeT b npoMewyTotHyio eMKOCTb seventh Ckopoctb nocTynJteHHH cenapHpyeMoficycneHBHH coCTaeJiaeT okoao 50 jt / TAC. Πρη οτομ cenapaTop 6 cjieayeT otHUiaTb ot uuiaMacootbbtctbbhho nocjte 0.75 tacoe. 4to <5h H3ee «aTb eoJibuiHX noTepb BHXoaa tepHciuiiH, iicpejiomhctkoB πρητοκ apoMuxeBoÍi cycnett3HH npexpauiaeToa h op3 cjiHea juta euxojta npojiyKTa ποηβητοπ HCTaa npo3patHaa Boaa. night amro BKAntaeTcaποτοκ boam jute ynpaBJiHHh h tiutaH bmboahtch tiapy> t <y. »2 npoMewyTOHHoB bmkocth 7 pacTBOp. coAepmauiHfl HHBepTa3y, πομοιιιη AoeaTopa 8ποαβοαητοη κ cjioeBOMy «HJtbTpy 9 c uejtbn ocseT-nanuieB« HJibTpauHH. ΒΑΘΗΗβ ΠΡΟΗΟΧΟΑΗΤ «ΛβΓΟΑβρΗ ΠΡΗΜβΗβΗΗ» CJIOBB «HJibTpa βΠβ KP-10 (40 X 40 CM) cnpoxoAHMOCTbn λα 450 Λ / tac.M2. To Itoom noBucHTb KatecTBo Hi HiibTpaTa. ocBeTJtanieaa ctopohbcaobb «HJIbTpa οβΚΑΒΑΜΒββτοπ noKpMBaioiueB« HJtbTpoBajtbHoB eyMarofi 291 (40 x 40 cm). ycTaHOBJTEHHNhhhhhhhhhhhhhhhhpnhhhhpphhhpnpnhp 20mmh. προμμβββτοη boaoB.

CxopocTb «HJtbTpauHH ycTaHaBAHBaeTca npHejtH3HTeJtbHO Ha 80-100 a / tac. MaiecHMaJibHaapa3HHua jtaBJteHHH Memay bxoaom to bmxoaom «HJibTpa πρη btom no aoAWHa npeauuiaTb 200-250 KPa.

CaohctmB «HAbTp 9 πρη 3TOM ycTpoen Takhte oepa3OM, HTO napaay npoueccoM C« H HJtbTpauHHΠΡΟΗΟΧΟΑΗΤ KpyFOOOOpOT ΟΡβΑΜ and HTO ΠΡβΑΟΤΒΡβΙΙΙββΤΟΗ ΒΟ3ΗΗΚΗΟΒΒΗΗΒ Η36ΜΤΟΜΗΟΓΟ AaBJteHHH.Moryuiero πρηβθοτη κ npewaeBpeMeHHOMy BMxoay H3 čtpoh MaTepHaaoB «HJibTpa.

OcTanuiHBca nocte UHpicyjiauHH octbtok ocBeTAaeMOFo. coaepiKauiero HHBepTa3y pacTBopa noc.neακοΗΗβΗΗΗ ocaeTAHMUteB «HAbTpauHH CHoea ββοαητοη b cenapaTop 6.

OepaeaTUBaeMuB aaaee pacTBOp ββοαητοη b npoMewyTotHyn θμκοοτβ 10th AaJtbiieBuiaa otHCTKa.KOHueHTpauHa h CTepHAHSauHa coAepwaiiiero HHsepTa3y pacTBopa προηοχοαητ b cneiiHaAbHofiΚΟΜβΗΗΗΡΟΒβΗΗοΒ yCTaHOBKe JUTH yJIbTpa- H MHKpO «HAbTpaUHH 11th noHeiuanmHBca Ha onope naxeT nAacTHH HcnoAb3yeTca πρη 5 m2« HJibTpoeajtbHoB njiomaAH jutayAbTpa HAbTpauHH * η πρη 4 m2 jute MHKpo * HAbTpauHH. Ojih ysjta yjtbTpa «HJtbTpauHOHHoB cenapauHH 12HcnoJtbsynTca MeMepaHU UF c nponycmtoB cnocenocTMo - 80 - 140 j / tac.M2 THna CAM - UF - 120 -CP (πρβΑΠΡΗΗ-ΓΗΗ βΕΒ UEJIBIIJTO®-yHfl-UEJIBBaj | LBEPKE ΒΗΤΤΕΗΒΕΡΓΕ). 3τοτ τηπ oeecnenHBaeTcejteKTHBHOCTb HHBepTa3M - eojtbuie 99 *. She is a man of HHMecKoB cTaeHJtbHocTH αοποαηητθαβηοMextAy MeMepaHoB h cepacuBanuieB lutacTHHoB «HJibTpa pacnoJtomeHa onopHaa TxaHb H3 ηθτκβηηογοΜβΤβΡΗβΑβ. ypejl MHKpO »HAbTpaUHH 13 OCHaittOH HAepilUM ΜΗΚΡΟΦΗΑΒΤΡΟΜ H3 CJtOMHOrO nOJ1H3 * Hpa (UeHTpajtbHuBHHCTHTyT and AepHHX HCCJteAOBaHHfi AxaAeMHH Hayx ΓΑΡ. PoCCeHAOp«) C AHaMeTpOM πορ OT 0.2 MM. 3τοτ «HJIbTpaUHOHHMB ΜβΤβρΗβΑΒ JUIH nOBUUieHHa CTaeHJIbHOCTH Taxwe HMeeT οπορημΒ CAOB H3 HeTKaHHOrOMaTepHana.

Ho npoMemyTOHHoB bmkocth 10 pacTsop, coAepmauiHB HHBepTaay. ρ 3 3 tep tep tep tep tep tep tep tep tep Har Har Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac Pac T Pac T Pac T T T T T T T. ΠΡΗΗβΜ MOJteKyjtU C MOJteKyjiapHUM 5,271 100 / /

BecoK Buuie 150,000 3aAepwHBaiQTC $ i.

Ckopoctb ctgkshhsi npo «HjibTPHpoBaHHoro pacTBopa πρη stoh - okojio 80 - 140 ji / Mac npw hcxoahom aaBJieiiHH 0.1 - 0.3 MPa. Ακτηβηοοτβ ηιιββρτη3Μ b npo H HJibTpHpoBaHHOM pacTeope πρη 3TOM HHwe 0.5%. nojiyMeHHbi nyTeH ocBeTJisnoiyefi «HJibrpauHH pacTeop πρη πομοικη yjibTpa * HJibTpauHH cryuiaeTcsi Ha1 / 10 oeteHa. ΚακπβΗτρβτ. noJiyMeHHuK nocjie yjibTpa «HjibTpauHH. Β ο co co co co co '1 co ej x co co co co co co co' '' '' no H H H H H H H ni H H H H. Π K OM H H K K H K K K K K K H H H H H H H H H n n n n n

TaM OTaejinnTcn coAepmauiHecti β κοΗΐιβΗτρβτβ aeuaTypHpoBaHHue προτθηηη η μηκροοργ «ηη3μη .noHHwaoiuHe xanecTBO npoayxuHH. BuuieHaaBaiiHaa and XTHBHan «HJibTPHpynuian njrouiaab ycTaHaejiHBaeTcflHa nponycx okojio 20 ji / Mac. oj b ο e e e e e e e e e e e e oj oj oj oj oj oj oj oj oj oj oj oj oj oj eojibuie. MeM 0.3 MPa. U MHUieHHufí. xoHueHTPHPOBeHHNR h cTepHJiHaoBaiiHNn pacTBop HiiBepTa3ii nocTynaeT β θμκοοτβ ajihCMceuiHBaHHsi η HarpeBaHHH 17 h tbm nepeMeuiHBaeTCH c rjtHiiepojtoM β οοοτηοιιιθηηη 1: 1 h to camumCTaeHJiHSHpyBTCH. Bhxoa cTa6HjiH3Hp <3BaHHoro pacTBopa HHBepTa3u - 90 xr <75% b unexpected HaHcnojfb3yeMue ακτηβηηθ xJieeHue apomukh). 271 100 6

«OPMXJIA H30BPETE1IHH ΗΟΤ ΤΟΛ ΤΟΛ I I I I I I O O O O O O O O KT KT KT KT KT KT KT KT KT KT KT KT. ΠΟ KOTOpOMyaxTHBHue XJieeHue aponiwH what cyxofi apowiKeBofi cyecTaHUHefi (HTS) ot 30 - 33% πρμ πομοιιι Boau cycneHflifby »Tcn npH <saH3HTeabHo Ha KJieTKH stack cycneH3HH HenpepbiBHMM cnocoeoM pa3pyiua »i $ Tc b3HaKOMOfi CTepWHeBoň MejlbHHlie 3 ΠΡΗ nOMOUlH MHKPOCTexaOUiaPHKOB ΛΗβΜβΤΡΟΜ B 1.2 MM 0.5 ΛΟ ΗMHCJIOM ΟβΟΡΟΤΟΒ Ρβ3ΜΘΙΒΗΒΗ» Ι1 | ΘΓΟ BSJía - 2500-4000 Οβ. / MHH.. nOCJie ΜβΓΟ B 3HaKOMOM CenapaTOpe 6 ncuiynanT psctbop. coaepmaiUHti HHBepTa3y, πρΗΗβΜ NEP yaaaeHHeM uuiaMa ocTanaBJiHBaeT «πρκτοκcycneH3HH. Potop and Tax aoaro npoMHBaeTca Boaofi. quinoxalin y suxoaa γοτοβογο npoayxTa not ποηβητοπMHCTan Boaa, nocą dimness pacTBop noneepraeTCH 3ΗβκθΜθΒ oceeTasnouieň« HabTpauHH πρη πομοιιιηcaoHCToro »HJibTpa ninth πρηηθμ cjioh« HabTpa HMepT nponycxHyn cnocoeHocTb ot 425-475 a / n.M2.ocBeTJianuiHe cjioh H abTpa aonoaHHTeabHO oeaarauTCH «HabTpoBaabHofi eyMarofi, and not to HaaoMocBeTanniuefi« HabTpauHH 3TH cjioh «HabTpa okojio 20 mhh. προΜΗΒβητοη Boaofi. ρ 3 ua 3 H H H H H H H H H H H H H H H H H T T T T T T T caoHc-ruft «HJibTp Tax ycTaHOB. hto Hapaay c npoueccoM «HabTpauHH BO3MO > KHa uHpxyaauHH. and ocTaioiunficaoctstok ocBeTJineMoro paCTBopa ηηβθρτββη chobb noaeepraeTca cenapauHH. nocae nero pacTBopBBoaHTca b xoMeHHHpoeaHHyn ycTaHoexy yabTpa- h MHKpo * HabTpauHH 11 tbkhm oepa30M. hto pacTBopciianaaa npoxoaHT pa3aeaanuiHin y3ea yabTpa «HabTpauHH 12. noaeepraeTca aHa * HabTpauHH πρηUHpxyaauHH. nocae aero npoxoaHT nepea y3ea MHKpo «HabTpauHH 13, nponycxHaa cnocoeHocTbHcnoJib3yeMMX b pa3aeaaniueM y3ae yabTpa« HabTpauHH ΜβΜβρβΗ UF - 80-140 a / n.M2. exoaHoe

aaejieHHe - 0.15 - 0.4. euxoaHoe aaeaeHHe - 0.1-0.3 MPa, and Meway ΜβΜβρβΗοιί h naacTHHaMH «HJibTpa noMeuiaeTCfl onopHaa TxaHb. HanpHMep, H3 HeTxaHHoro MaTepHaaa. pacTBop b pa3aeaanuieMysae yjibTpa * HjibTpauHH 12 KOHaeHCHpyeTca at 1/10. nocae nero KOHueHTpaT b cooTHOiueHHH 1 '· 1pa3eaeaaeTca aoaoK η Tax aoaro aHa «HHbTpHpyeTca cnocoeoM UHpxyaauHH, noxa οππτβ aocTHraeTcaHcxoaHuti oebeM xonueiiTpaTa. nocae nero OMHiueHHufi pacTeop HHBepTasu npoxoaHT nepe3 paaaeaanuiHfiy3ea MHxpo * HabTpauHH 13. a «HabTpoeaabHuH MaTepnaa Tax we.xax h b pa3aean» iueM y3ae yabTpa «HabTpauHH 12 cHaemeH x ο ρ x x x x x x x x ac ac H ac ac po a noaBoaHTca noaCTaHaapT h npHBoaHTcn b ceputhiuft BHa. and

Claims (1)

BACKGROUND OF THE INVENTION Approximately 10 yeast cells are a method of blister production of an active baker's yeast in which sealed baker's yeast with a dry weight of 30 to 33% by weight is suspended by water and the temperature is adjusted to 10-12 ° C. characterized in that the slurry is continuously disintegrated by means of a sloping mill of 0.5 to 1.2 mm diameter and a stirring shaft speed of 2500 to 40000 rpm, then a solution containing the invertase is obtained in the separator, the feed is discontinued prior to sludge removal. the slurry, and the rotor is washed with water for as long as pure water does not appear at the exit of the finished product, then the rotor is subjected to known fining filtration by means of a laminated filter, wherein the filter layers have a permeability of from 400 to 475 l / hr. m2. the filter clearing layers are additionally lined with filter paper, and before filtering starts, these filter layers are washed with water for about 20 minutes, with a pressure difference between the filter inlet and filter outlet of 200 to 250 kPa. that, together with the filtration process, circulation is possible, and the remainder of the refined solution of invertase is again subjected to separation, then the solution is introduced by the combined ultra and mlrofiltration in such a way that the solution initially passes through the separation stage and is subjected to flocculation during circulation and then passing through the node. wherein the permeability of the membranes used in the distribution node is UF - 80-140 L / hr. m2. the inlet pressure is 0.15 to 0.4 MPa. the outlet pressure is 0.1 to 0.3 MPa. and a supporting fabric, such as a nonwoven material, is placed between the membrane and the filter plates, the solution is condensed to 1/10 in the ultrafiltration node, and the concentrate is diluted 1: 1 with water and circulated for a long time until the initial volume is reached again concentrate, wherein the purified solution of the vertase passes through the dissolution node of the microfiltration, wherein the diameter of the substance used in the microfiltration is 0.2 µm, the inlet pressure is at most 0.3 MPa, and the filter substance is likewise equipped with a backing fabric in the ultrafiltration node; stabilizes in a known way, and standardizes it. 1 drawing