CS268591B1 - The method of obtaining enzymes from the feraentation base of penicillin production ultra f 1 lt rieloti - Google Patents

Abstract

The purpose of the ultrafiltration is to remove enzymes, especially proteins, from the fermentation broth of penicillin production by ultrafiltration. Ultrafiltration is used to isolate and concentrate undesirable substances from the fermentation broth in the final product to a concentration in which they are practically useful. At the same time, the final product penicillin is purified from undesirable and acroaceutical substances.

Description

EN 268 591 B1 1

Inventing a commercially useful enzymes from feraentative pellets from penicillin production by ultra raffle.

The pharmaceutical production of benzylpeniline and phenoxyethyl ethylidone is carried out subaerially by the Penldlllua chrysogen. The main carbon source and the feraentative post are u-charose, glucose, or lactose, the source is corn extract, bleach sub-strata, acacia and soybean. In addition, the pod contains trace elements, biologically active substances, precursors, buffering agents, defoamers.

The culture was filled at 25 ° C, the pH ranged from 6 to 7.5. The stage is intensely alive and airy. The culture time is 5-8 days. After the feraeration process has been completed, the feraentation adultera is filtered on wheels or rotary flasks to remove insoluble matter.

During the culture, the production kaen forms a penicillin eite with additional substances and also extracellular enzymes, the presence of which in the product and the product is non-sweet, to the extent that it does not cure to particularly sensitive substances. These substances can be separated from the feraentation stage by ultrafiltration through an ultrafiltration and a membrane with a separating edge of 5,000 - 50,000 and molecular weight, which retains the high molecular weight substances and, on the other hand, transfers the low molecular weight components of the phaeractic substrate. From the high molecular weight concentrate obtained by ultrafiltration, it is possible to obtain, in the meantime, importantly important enzymes, especially proteases. EXAMPLE 1 1 000 000 parts of a benzyl penicillin feraentation process containing 1 x ultrafilera ultrafiltration through ultrafiltration and a membrane with a 10,000 molecular weight cutoff at 25 ° C and 0.3 HPa pressure. The extrudate is separated by 995,000 parts percolate containing a low molecular weight substance and 5,000 parts of the concentrate containing 1 µl of enzyme are left over the membrane. Purified peracyl free peracid was used to produce benzylpiperidine. The ultrafiltrate concentrate is used to produce enzymes. Example 2 1 000 000 parts of a phenoxy-ethyl-pennyl feraentation filter containing 5 parts of ultrafiltrate enzymes via ultrafiltration and a barrier having a 20,000-molecular haotnoatl cut-off at a temperature of 15 ° C and a pressure of 0.4 MPa. The flow is separated by aO000,000 parts percolate containing a low molecular weight substance and 10,000 parts of the concentrate containing 5 parts of the enzyme remain above the membrane. The purified percolate-free peracetic acid was used to produce phenoxy-ethylpolymer. The concentrate is used for the production of enzymes.

Claims (1)

CS 268 591 B1 SUMMARY OF THE INVENTION The process of extruding enxys with ultra-thin-layer fabrication of panes to produce the 1,000,000 pellets of pertantains and the production of pansions of 1 to 50 pellets of enzymes and ultrafiltration of the cex semi-ultrafiltration and debonding cracks 5 5,000 to 50,000 and a pressure of 0.1 to 0.5 HPa and that of the cex and the membrane separates 800,000 and 995,000 of the pellets pertaining to the nixcoaolecular substances, leaving 5,000 to 200,000 of the 1 to 10,000 concentrate concentrates above the alabaster. 50 d1 * hunting of enzymes.