CS268090B1 - A synergistic agent for controlling plant growth, in particular plant organogenesis and branching - Google Patents

Abstract

For the above purpose, purine cytokinins have been used so far, the disadvantages of which are complex synthesis, high purchase price and relatively low efficiency in plant varieties with a high degree of apical dominance. In contrast, the active ingredient of the composition according to the invention is N-phenyl N'-1,2,3-thisdiazol-5-yl-urea (0.01 to 10 mg per liter) in a mixture with 6-/metahydroxybenzylamino/-purine (0.01 mg to 1 mg per liter). The application is carried out by watering, spraying or aerially, the composition alone or in a mixture with pesticides, fungicides, nitrogen fertilizers, especially urea. This is a composition with high cytokinin efficiency and low purchase price.

Description

The invention relates to a synergistic composition for regulating plant growth, in particular organogenesis and plant branching.

Purine cytokinins (zeatin, kinetin, 6-benzylaminopurine and its derivatives) are still used for this purpose. The disadvantage of these agents is their complex synthesis (consequently high purchase price) and relatively low efficiency in plant species and varieties with a high degree of apical dominance.

This disadvantage is eliminated by the object according to the invention, the essence of which is that its active ingredient is a mixture of N-phenyl-N'-1,2,3-thiadiazol-5-yl-urea in an amount of 0.01 mg to 10 mg, and 6- (metahydroxybenzylamino) -purine (hereinafter m-OH-BAP) in an amount of 0.01 mg to 1 mg per liter of nutrient medium or spray mixture.

N-phenyl-N-1,2,3-thiadiazol-5-yl-urea (urea cytokinin = MC) is the active ingredient of the composition according to the invention and is also used alone as a means of stimulating shoots in cereals and lateral buds in plants ( author's certificate 247 307). When applied to whole plants in field conditions or in greenhouses by spraying or watering in addition to the stated concentrations of active ingredient, the concentration of the mixture according to the invention can be increased according to the plant species (especially due to low water consumption per hectare). cheap.

The aqueous solution of the mixture can be applied by various sprayers and also by air. These solutions can also be applied in a mixture with pesticides and fungicides and with nitrogen fertilizers, especially urea. The increase in protein content in the green parts and organs of the plant occurs within 1 week after application (spraying on the leaf, etc.). For fruit trees, it is possible to increase the branching of i, annual shoots (application in spring) and the establishment of flower buds (application in summer), and thus speed up and increase the harvest in the following year. For forest trees, branching and increments of wood can be increased. The subject of the invention further serves to stimulate the deganogenesis and branching of plant explants, the formation of adventitious buds and emryoids from calli. .

The following are examples of the application of the stimulation agents according to the invention.

I - Example 1

I If it was made on a raspberry variety LS 33/11, which has a high apical dominance and therefore poorly branched on a nutrient medium containing commonly used purine cytokinins. There were 10 flasks in each experimental variant and 4 explants in each flask. In the control variant (6-benzylaminopurine 2 mg per liter of nutrient mixture), branching was insufficient,

- Even though after 5 weeks (1 passage) the average number of side twigs was only 1.57 + 0.17

I (100%). In contrast, in the variant according to the invention (urea cytokinin 0.1 mg per liter

I + 6- (metahydroxybenzylamino) -purine 0.5 mg per liter / increased the number of newly grown ’I shoots’. In the course of one explant it was 2.933 - 0.1, ie 106% compared to the control. Due to the fact that explants in meristem culture multiply geometrically,

I newly proposed biotechnology means economic benefit, because after 1 fist

Even the season (about 5 to 6 passages) can amount to about a 10-fold increase in the number of uncultivated exI plantations in this variety. By further increasing the MC concentration above 0.1 mg per 1, the effect

I 'm not increasing.

Example 2

If it was performed on potted poinsettias (origin from Semper-Tusimice), the leafy branches of which were sprayed with an aqueous solution of a mixture of MC P5 mg per liter of water) and 6- (metahydroxybenzylamino) -purine (5 mg per liter of water). The plants were grown in greenhouse conditions during the summer on the natural length of the day. Two weeks after spraying, most of the lateral buds that grew further into the twigs emerged. On the control branches, only in aqueous solution, the lateral buds did not sprout in the indicated experimental conditions. The composition according to the invention thus caused premature ejection of the side branches. As the sprayed branches, whose leaves were greener and heavier, also intensified, it was decided to determine the soluble proteins in these experimental branches in comparison with the control ones (Bradford method). The result of the analysis showed a slight increase in the protein content in the sprayed twigs (21.80 mg protein per mg pellet), while in / the control was only 17.35 mg protein per mg pellet.

Claims (1)

OBJECT OF THE INVENTION A synergistic agent for regulating plant growth, in particular organogenesis and plant branching, characterized in that its active ingredient is a mixture of N-phenyl-N-1,2,3-thiadiazol-5-yl-urea in an amount of 0.01 mg to 10 mg and 6- (metahydroxybenzylamino) -purine in an amount of 0.01 mg to 1 mg per liter of nutrient medium or spray mixture.