CS258414B1 - Method of nourserotricine fermentation production - Google Patents

Method of nourserotricine fermentation production Download PDF

Info

Publication number
CS258414B1
CS258414B1 CS84355A CS35584A CS258414B1 CS 258414 B1 CS258414 B1 CS 258414B1 CS 84355 A CS84355 A CS 84355A CS 35584 A CS35584 A CS 35584A CS 258414 B1 CS258414 B1 CS 258414B1
Authority
CS
Czechoslovakia
Prior art keywords
ηπη
sterilization
φοοφβτβ
fermentation
nph
Prior art date
Application number
CS84355A
Other languages
Czech (cs)
Inventor
Peter-Juergen Mueller
Gottfried Gaubold
Harald Bocker
Hans-Helmut Grosse
Michael Menner
Original Assignee
Mueller Peter Juergen
Gottfried Gaubold
Harald Bocker
Grosse Hans Helmut
Michael Menner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mueller Peter Juergen, Gottfried Gaubold, Harald Bocker, Grosse Hans Helmut, Michael Menner filed Critical Mueller Peter Juergen
Publication of CS258414B1 publication Critical patent/CS258414B1/en

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Fodder In General (AREA)
  • Compounds Of Unknown Constitution (AREA)

Description

IAND

HaseaHHe Η3θ6ρβτβΗΗΗHaseaHHe Η3θ6ρβτβΗΗΗ

Cnoco6 nonyHeHHH HoypaeoTpHUHHa OCnaCTb ΠρΗΜβΗβΗΗΗ Η3θ6ρβΤβΗΗΗ Η3θ6ρετβΗΗβ OTHOCHTCH K nOJiyHeHHJO ΟΤρβΠΤΟΤρΗΙίΗΗΟΒΟΓΟ ΒΗΤΗβΗΟΤΗΚβ HOypaeOTPH“ΗΗΗβ πγτβΜ uejienanpaBJíeHHoro BO3fleňcTBHH Ha MHKpoGHonormecKyio φερΜβΗΤΗΗΗ».H3o6peTeHne ΗΜεετ 3havenue %ηη MHKpoCHOJiorHMecxoft npoMbmuieHHOCTH.Cnoco6 nonyHeHHH HoypaeoTpHUHHa OCnaCTb ΠρΗΜβΗβΗΗΗ Η3θ6ρβΤβΗΗΗ Η3θ6ρετβΗΗβ OTHOCHTCH K nOJiyHeHHJO ΟΤρβΠΤΟΤρΗΙίΗΗΟΒΟΓΟ ΒΗΤΗβΗΟΤΗΚβ HOypaeOTPH "ΗΗΗβ πγτβΜ uejienanpaBJíeHHoro BO3fleňcTBHH Ha MHKpoGHonormecKyio φερΜβΗΤΗΗΗ» .H3o6peTeHne ΗΜεετ 3havenue% ηη MHKpoCHOJiorHMecxoft npoMbmuieHHOCTH.

XapaKTepwcTHKa H3BecTHbix TexHnuecKHX peuieHHň B CBH3H C 6HOCHHTe3OM BTOpHHHblX MeTaGOJIHTOB B JIHTepaType yKaaWBaiOT Ha TpH .ocHOBHbix 4>aKTOpa penpeccHBHoro bjihhhhh , oxasbiBeeMoro 1. rnioK03oň h npyrHMH βηλπμη caxapa 2. aMHHOKHCJIOTaMH H aMMOHHňHblMH COJIHMH H · 3. φοεφβτοΜ (Martin, J.F. h'A.L. Demaín: Control of antibiotie biosynthesis. Microbiol,XapaKTepwcTHKa H3BecTHbix TexHnuecKHX peuieHHň CBH3H B C B 6HOCHHTe3OM BTOpHHHblX MeTaGOJIHTOB JIHTepaType yKaaWBaiOT Ha TPH .ocHOBHbix 4> aKTOpa penpeccHBHoro bjihhhhh, oxasbiBeeMoro first rnioK03oň h npyrHMH βηλπμη caxapa second aMHHOKHCJIOTaMH H · H aMMOHHňHblMH COJIHMH third φοεφβτοΜ (Martin JF h'AL Demain : Control of antibiotics biosynthesis.

Rev. 44, 230-251 (1980)). B cnyuae GHocHHTeaa MHKpoopraHH3MOM Streptomyces noursei IMET JA 3890 b ΟΤρβΠΤΟΤρΗΙίΗΗΟΒΟΓΟ ΒΗΤη6Η0ΤΗΚΒ HOyp3eOTpHBHHa H3BeCTHO, MTO aMHHOKHCJlOTbl HaMMOHHňHbie cojih οτρΗΐχβτβΠΒΗΟ bjihhmt na 3TOT GHOcHHTes (Gráfe U., Stendal. A.,Bocker, Η. ,h Thrum, H: Regulative intluence Of o-aminobenzoic acid (OABA)on the biosynthesis of noursethricin in cultures of Streptomyces nourseiJA 3890 b, Z. Allg. Mikrobiol. 20, 185-194 (1980) h iiHTHpyeMaH τβΜ xe JiHTe—paTypa). ‘Roar. 44, 230-251 (1980)]. B cnyuae GHocHHTeaa MHKpoopraHH3MOM Streptomyces noursei IMET JA 3890 b ΟΤρβΠΤΟΤρΗΙίΗΗΟΒΟΓΟ ΒΗΤη6Η0ΤΗΚΒ HOyp3eOTpHBHHa H3BeCTHO MTO aMHHOKHCJlOTbl HaMMOHHňHbie cojih οτρΗΐχβτβΠΒΗΟ bjihhmt on 3This GHOcHHTes (Chart U. Stendal. A., Bocker, Η., H THRU H Regulative intluence Of o-aminobenzoic acid (OABA) on the biosynthesis of noursethricin in cultures of Streptomyces nourseiJA 3890 b, Z. Allg., Microbiol 20, 185-194 (1980). '

Iloxa b xavecTBe ηΟτομηηκβ aaoTa b nHTaTenbHbix cpejiax HcnojibaoBanH ΗΗτρβτ aM- -ΜΟΗΗΗ (cm. Gráfe et ál. 1980). B stom coepjiHeHHH nojiOBHHa aaÓTa He npeflCTaB-JieHa B ΒΗηβ HOHOB aMMOHHH. ' "Iloxa b xavecTBe ηΟτομηηκβ aaoTa b nHTaTenbHbix cpejiax HcnojibaoBanH ΗΗτρβτ aM- -ΜΟΗΗΗ (cm. Gráfe et al. 1980). B stom coepjiHeHH nojiOBHHaaaTa He npeflCTaB-JieHa B ΒΗηβ HOHOB aMMOHHH. ''

HeflOCTaTKOM ΤβΧΗΗΗβΟΚΟΓΟ ΠρΗΜβΗβΗΗΗ HBJlHeTCH TO, HTO HHTpaT aMMOHHH ΗΒΠΗβΤ—ch BspbiBHaTbiM BemecTBOM h ncnojib3OBaHne ero flonycxaeTCH tojibko b coveTaHHHc TpyfloeMKHMH MepaMH 6e3onacHocTH. KpoMe τογο, y^ajiocb oSHapyxHBaTb BφΗΠΜ'ρβτε xyjibTypbi b Konye jiaSopaTopnoií φβρΜβΗτβΐίΗΗ He Conee 300 μκγ Hoyp- 258414 2 3eoTpHUHHa Ha μπ Tojibxo npH npHSaaneHHH oTHocHTejibHO floporHX a Bpefíoax. nnfi ,oxpyxcaiomeň cpeflbí ηηγη6ητοροβ AbixaHHH h nepenoca ημηηοχηοποτ, HanpHMep,o-aMHHo6eH3oňHoň KHcnoTbi (OABA) (Grafe, U., Bocker, H,, Reinhardt, G. κThrum, H.: Regulative Beeinflussung der Nourseothricinbiosynthese durcho-Amínobenzoesaure in Kultuten des Streptomyces nourséi JA 3890 b. Z. Allg.Mikrobíol. 14, 659-673 (1974)). H3BeCTHO, HTO H36bITOHHblň φοεφβΤ OTpHAaTeJIbHO ΒΠΗΗβΤ Ha : 6HOCHHTe3 pH/ja bto*PHMHbix MeTaSojiHTOB (Martin, J.F. : Control of antibíotíc bíosynthesis by *phosphate. Adv. Biochern. Eng, 6, 105-127 (1977); Weinberg.D.: Secondary me-tabolism: regulation by phosphate and trace elements, Folia Microbiol,. 23,496-504 (1978)). IIoaTOMy, HexoTopwe TexHHHecKue nponeccw Mnxpo6HOJiornnecKoftφβρΜβΗΤΗΐχΗΗ flJIH ΠΟΠΥΊβΗΗΗ βΗΤΗβκΟΤΗΚΟΒ npOBOflHT npH ΚΟΗΑβΗΤρ ΑΐχΗΗΧ φΟεφβΤβ,cyfíonTHManbHbix απη ροοτβ MHxpoGHonorHHecxoro npoAyneHTa. B TexHHHecxtix φερηMeHTaiwHx απη nojiyueHHH BTopHHHbix ΜβτββαπΜτοΒ, xax npaBuno, HcnontsyroT Ta—KHe KOMnoHeHTbi cpeflbí pacTHTenbHoro η χοϊβοτηογο nponcxoxAeHHH, xax xpaxMan,coeBbiň ηιροτ, xyxypysHbiii sxcTpaxT, Menacca, Mstctíoň axcTpaxT η AP· 06®ee co-AepwaHHe φοοφβτε h GHonorHHecxas AocTynHOcTb (pacTBOpHMocTb) KoMnnexcHorocy6cTpara pa3AHHHoe b 3aBHcnMocTH ot ero npoHcxoxfleHHH h oGpaGoTXH.HeflOCTaTKOM ΤβΧΗΗΗβΟΚΟΓΟβΗΗΗ HBJlHeTCH TO, HTO HHTpaT aMMOHHH ΗΒΠΗβΤ —ch BspbiBHaTbiM BemecTBOM h nbib3OBaHne ero flonycxaeTCH tojibko b coveTaHHHc TpyfloeMKHMH MepaMH 6e3onacHocTH. C apy apy apy apy apy apy apy apy apy apy apy apy apy C C C C C C C C C C C C oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj nnfi, oxpyxcaiomeň cpeflbí ηηγη6ητοροβ AbixaHHH h nepenoca ημηηοχηοποτ, HanpHMep, o-aMHHo6eH3oňHoň KHcnoTbi (GABA) (Graf, U., Bocker, H ,, Reinhardt, G. κThrum, H .: Regulative Beeinflussung der durch Nourseothricinbiosynthese-Amínobenzoesaure in Kultuten des Streptomyces nourséi JA 3890 b. Z. Allg. Microbiol., 14, 659-673 (1974). H3BeCTHO, HTO H36bITOHHbl φοεφβΤ OTpHAaTeJIbHO ΒΠΗΗβΤ Ha: 6HOCHHTe3 pH / ja bto * PHMHbix MeTaSojiHTOB (Martin, JF: Control of antibiotic biosynthesis by * phosphate. Adv. Biochern. Eng, 6, 105-127 (1977); Secondary metabolism: regulation by phosphate and trace elements, Folia Microbiol., 23,496-504 (1978). IIoaTOMy, HexoTopwe TexHHHecKue nponeccw Mnxpo6HOJiornnecKoftφβρΜβΗΤΗΐχΗΗ flJIH ΠΟΠΥΊβΗΗΗ βΗΤΗβκΟΤΗΚΟΒ npOBOflHT npH ΗΤΑβΗΤρ ΑΐχΗΗΧ φΟεφβΤβ, cyphionTHManbHbix απη ροοτβ MHxpoGHonorHHecxoro npoAyneHTa. B TexHHHecxtix φερηMeHTaiwHx απη nojiyueHHH BTopHHHbix ΜβτββαπΜτοΒ, xax npaBuno, HcnontsyroT Khe Ta-KOMnoHeHTbi cpeflbí pacTHTenbHoro η χοϊβοτηογο nponcxoxAeHHH, xax xpaxMan, coeBbiň ηιροτ, xyxypysHbiii sxcTpaxT, Menacca, Mstctíoň axcTpaxT η · AP 06®ee co-AepwaHHe φοοφβτε h GHonorHHecxas AocTynHOcTb (pacTBOpHMocTb ) CoMnnexcHorocy6cTpara pa3AHHHoe b3aBcnMocTH otro npoHcxoxfleHHH h oGpaGoTXH.

TaxHM o6pa3OM, oGmuň φσοφετ pa3jiHMHoro coÁepxtaHHH, nepeHocHMbiň nepes xom-nJieKCHbíe cyGcTpaTbi b cpeny rnyfíHHHoro xyjibTHBHpoBaHHH, npHcyTcTByeT b nana-ne φβρΜβΗΤΘΗΗΗ B BHfle paCTBOpeHHOrO, ΗβΜβΑΠβΗΗΟ flOeTynHoro φοεφβτβ. ΠρΗΒβΑβΗΗΜβ oGcTOHTenbCTBa npHi .(.HnuanbHo aaTpyflHHioT cTaHflapTH3aun» nHT.aTenb-bbix cpeff. EHOJioruvecKaa flocTynHOCTb φοοφβτε ΗΜββτ pemaiomee SHaneime ffiut bm-xofla wenaeMbix BTOpHHHbix ΜβτβδοηΗτοΒ, Tax xax, c ogHoft cTOpOHbí, onpegeneHHoeKojiHvecTBO φοεφβτβ HeoGxoAHMo απη počija MHxpoopraHH3MOB—npoAyneHTOB, a, cAPyroň cTopoHbí, cjihiukom Bbicoxne HauajibHbie KOHAeHTpauHH pacTBopHMoro φοοφβτβMoryT yrHeTaTb oGpasoBaHHe BTopnvHbix MeTaGonuTOB., Πβτθητ DD-WP 155 239npeTeHAyer Ha npHMeneHHe xoHneHTpanHH coAepxamHxcn b xyjibTypaJibHoft xhakocthφοοφετΗΗχ hOhob xax perynHpyeMy» BenHHHHy ρ,ηη cTaGunnsamui MHXpoGHonorHuec-xoň xyrtbTypbi HenpepbiBHoro AeňcTBHH. 3tot cnocoG, oflHaxo, othochtch τοπβκοκ φερΜβΗτβτΗΒΗΟΐ^ nojiyneHHio GHOMaccbi, npnHeM aanaHHoe 3HaHeHHe KOHiteHTpauHHφοεφβτβ cneAyeT ycTáHaanHBaTb b npenenax 20-60 μγ/π φοοφβτβ. ΠρΗΜβΗβΗΗβ sto—to H3o6peTeHHH κ peryjiHpOBaHHio GHOCHHTeaa βτορηηηηχ ΜβτβθοπκτοΒ, b waČTHOc-TH B ΜΗΧροθΗΟΠΟΓΗΗβΟΧΗΧ XyHbTypaX C nepHOAHWeCXHM ΠΗΤΗΗΗβΜ, HeH3BeCTHO«3φφεχτω perynHposaHHH φοεφβτβ Ha nponecc 6HOcnHTe3a Hoyp3eoTpnAHHa HeHSBecT—HbI,TaxHM o6pa3OM, oGmuň φσοφετ pa3jiHMHoro coÁepxtaHHH, nepeHocHMbiň nepes Xom-nJieKCHbíe cyGcTpaTbi b cPen rnyfíHHHoro xyjibTHBHpoBaHHH, nana-b npHcyTcTByeT not φβρΜβΗΤΘΗΗΗ B BHfle paCTBOpeHHOrO, ΗβΜβΑΠβΗΗΟ flOeTynHoro φοεφβτβ. ΠρΗΒβΑβΗΗΜβ oGcTOHTenbCTBa npHi. (. HnuanbHo aaTpyflHHioT cTaHflapTH3aun »nHT.aTenb-bbix cpeff. EHOJioruvecKaa flocTynHOCTb φοοφβτε ΗΜββτ pemaiomee SHaneime ffiut BM-xofla wenaeMbix BTOpHHHbix ΜβτβδοηΗτοΒ, Tax XAX c ogHoft cTOpOHbí, onpegeneHHoeKojiHvecTBO φοεφβτβ HeoGxoAHMo απη počija MHxpoopraHH3MOB-npoAyneHTOB and, cAPyroň cTopoHbí, cjihiukom Bbicoxne HauajibHbie KOHAeHTpauHH pacTBopHMoro φοοφβτβMoryT yrHeTaTb oGpasoBaHHe BTopnvHbix MeTaGonuTOB., Πβτθητ DD-WP 155 239npeTeHAyer Ha npHMeneHHe xoHneHTpanHH coAepxamHxcn b xyjibTypaJibHoft xhakocthφοοφετΗΗχ hohoba XAX perynHpyeMy »BenHHHHy ρ, ηη cTaGunnsamui MHXpoGHonorHuec-XON xyrtbTypbi HenpepbiBHoro AeňcTBHH. 3This cnocoG, oflHaxo, othochtch τοπβκοκ φερΜβ ,τβτΗΒΗΟΐ ^ nojiyneHHio GHOMaccbi, npnHeM aanaHHoe 3HaHeHHe KOHiteHTpauHHφοεφβτβ cneAyeT ycTáHaanHBaTb b npenenax 20-60 μγ / π φοοφβτβ HHH κ peryjiHpOBaHHio GHOCHHTeaa βτορηηηηχ ΜβτβθοπκτοΒ b waČTHOc TH-B ΜΗΧροθΗΟΠΟΓΗΗβΟΧΗΧ XyHbTypaX C nepHOAHWeCXHM ΠΗΤΗΗΗβΜ, HeH3BeCTHO «3φφεχτω perynHposaHHH φοεφβτβ Ha nponecc 6HOcnHTe3a Hoyp3eoTpnAHHa HeHSBecT-HBI,

Renb H3o6peTeHHHRenb H3o6peTeHHH

IfejibK) Η3θ6ρετβΗΗΗ HBJíHeTca ycTpaHeHHe HenocTaTkoB paHee onHcaHHbix cnocoGoBnonyneHHH Hoyp3eoTpHAHHa h yBejiHveHHe xOHneHTpanHň HoypaeoTpHAHHa b τθχηη-necKHX φβρΜβΗΤθίξΗΗχ nyreM BO3AeňcTBHH Ha npoyecebi φερΜβΗτβιΐΗΗ. |H3JI0»eHHe CyiUHOCTH Η3θ6ρθΤβΗΗΗ B ocHOBy H3o6peTeHHH nonoweHa eanana npeACTaBHTb TexHHnecxH BbiroflHbift cnoco6AJiH npoMbimneHHoro npoH3BOAČTBa HoypaeoTpHAHHa. HeoxHAaHHO 6ωπο HaňAeHO, htoπρπ φβρΜβΗΤβΗΗΗΧ flJlH nOnyHeHHH HOypaeOTpHAHHa nocne ΠΟΗΗΧβΗΗΗ HandjIbHOňxoHneHTpaAHH φοοφβτβ HHXce cHHTaBnierocH paHee onTHManbHbíM SHaueHHH h nocne-Ay»mero npHGaBneHHH cooTBeTCTBywuiero ηπη Gonee Bbicoxoro xonHHecTBa φοοφβτβb npoAecce xynbTHBHpOBaHHH, A°6aBJiHeMoro no onpeAeneHHOMy pexcuMy ΒρβΜβΗΗ,nonynaioTCH 3HanHTenbHO Gonee BbicoKHe Bbixona Hoýp3eoTpnnnHa, 258414 3 ΚροΜβ τογο 6bino HaňflGHO, πτο b CaCOj— coflepwauteň nHTaTenbHoft cpene npH sa-M6H6 HCTOMHHKa a3OTa HHTpaTa aMMOHHH Ha cynbiJiaTcoAepxaiiiHft cynb4>aT ημμοηηηb cooTHomeHHH, CTexHOMeTpHMecKOM b othoqichhh coflepxaHHH aaoTa, HananbuaHKOHiíeHTpauHH φοεφβτβ cHHwaeTCH ót 15 μγ/π φοεφβτΗΟΓΟ P flo 1-3 μγ/π b coO-CTBeHHO H6H9M6HHOÍÍ cpefle nocjie CTeptUIHSaitHH BOffHHbiM napOM ΠΟΑ ΑβΒΠβΗΗβΜ. B oSmeM HaMajibHyw KOHueHTpauHio pacTBopHMoro, Οηοπογηηθοκη ΗβΜβΑΠβΗΗΟ flocTyn-Horo φοεφβτβ b npocTepwiH3OBaHHoft cpe#e moxho CHHSHTb cooTBeTCTBywmHM cor-nacoBaHneM pexHMa creptuíMsamiH h coAepxaHHH cyπbφaτHbκ hohob h HeopraHHHec-khx coneň ηπη hohob ΜβτβπποΒ, flaianHX bmgctg c φοεφβΤΗΜΜΗ ηπη cyj^aTHbíMHHOH3MH TpyflHO paCTBOpHMbie OCaflKH. ΠρΗΜΗΗΟή chhxghhh Taxoň HaMajibHoň KOHiteHTpaiíHH b cnynae npHcyTCTBHH AOCTa-ΤΟΗΗΟΓΟ KOJIHHeCTBa εγΠΒφβΤΗΕΙΧ HOHOB OKaaanCH CflBHr KHCJ1OTHOCTH CJia6OKHCnoňnHTaTejibHoň cpe/ju b CTopoHy menoMHhix 3HaneHHň pH b npouecce cTepHnH3auHH,Bbi3biBaeMbiň ocaxneHHeM CaS04 u οβη33ηημμ c sthm yuaneHHeM hs cpaam φΗ3Ηθπο-rmeCKH KHCJIbK ΟνΠΒφβΤΗΜΧ HOHOB. COOTBGTCTBeHHO HaHajlbHan KOHmeHTpaUHHpacTBopHMoro ΗΠΗ 6ΗΟΠΟΓΗΗβCKH flOCTyiIHOrO φοοφβτβ CHHMaeTCH TaKXe BCJieACTBHeycTaHOBKH tuenouHoro sHaueHHH pH nepeA cTepnnn3auHeň. yBenmeHHe 3HaqeHHH pHoeecneHHBaeT coagaHHe CnaronpHHTHbix ycnoBHň ρ,ηκ ocaxAeHHH φοοφβτβ c onpene-neHHbíMH npHcyTCTBynatHMH b nHTaTenbHoft cpefle ηοηθμη ΜβτβπποΒ. OcaxAeHH» φοο-φβτ coAepxanufX ocaAKOB cnocoCcTByeT HanpaeneHHOe πρΗθββπβΗΗβ tbkhx hohob Me-TajuioB, khk, HanpHMep, xeneao (III), antoMHHHň (III), MarHHft (II), KanbUHft(II), ahhk (II) h APyrne.IfejibK) Η3θ6ρετβΗΗΗ HBJíHeTca ycTpaHeHHe HenocTaTkoB Pahea onHcaHHbix cnocoGoBnonyneHHH Hoyp3eoTpHAHHa h yBejiHveHHe xOHneHTpanHň HoypaeoTpHAHHa b τθχηη-necKHX φβρΜβΗΤθίξΗΗχ Nyree BO3AeňcTBHH Ha npoyecebi φερΜβΗτβιΐΗΗ. | H3JI0 »eHHe CyiUHOCTH Η3θ6ρθΤβΗΗΗ B ocHOBy H3o6peTeHHH nonoweHa eanana npeACTaBHTb TexHHnecxH BbiroflHbift cnoco6AJiH npoMbimneHHoro npoH3BOACTBa HoypaeoTpHAHHa. HeoxHAaHHO 6ωπο HaňAeHO, htoπρπ φβρΜβΗΤβΗΗΗΧ flJlH nOnyHeHHH HOypaeOTpHAHHa nocne ΠΟΗΗΧβΗΗΗ HandjIbHOňxoHneHTpaAHH φοοφβτβ HHXce cHHTaBnierocH Pahea onTHManbHbíM SHaueHHH h nocne-Ay »mero npHGaBneHHH cooTBeTCTBywuiero ηπη Gone Bbicoxoro xonHHecTBa φοοφβτβb npoAecce xynbTHBHpOBaHHH, A ° 6aBJiHeMoro well onpeAeneHHOMy pexcuMy ΒρβΜβΗΗ, nonynaioTCH 3HanHTenbHO Gone BbicoKHe Bbixona Hoýp3eoTpnnnHa, 258414 3 ΚροΜβ τογο 6bino HaňflGHO, πτο b CaCOj- coflepwauteň nHTaTenbHoft cPen Nph-M6H6 HCTOMHHKa a3OTa HHTP aMMOHHH Ha cynbiJiaTcoAepxaiiiHft cynb4> aT ημμοηηηb cooTHomeHHH, CTexHOMeTpHMecKOM b othoqichhh coflepxaHHH AAOT, HananbuaHKOHiíeHTpauHH φοεφβτβ cHHwaeTCH formylimidazolyl 15 μγ / π φοεφβτΗΟΓΟ P flo 1-3 μγ / π b coO-CTBeHHO H6H9M6HHOÍÍ cpefle nocjie CTeptUIHSaitHH BOffHHbiM napOM ΠΟΑ ΑβΒΠβΗΗβΜ. B eighth HaMajibHyw KOHueHTpauHio pacTBopHMoro, Οηοπογηηθοκη ΗβΜβΑΠβΗΗΟ flocTyn-Horo φοεφβτβ b npocTepwiH3OBaHHoft CPE # e Moxhe CHHSHTb cooTBeTCTBywmHM cor-nacoBaHneM pexHMa creptuíMsamiH h coAepxaHHH cyπbφaτHbκ hohoba h HeopraHHHec-khx Conen ηπη hohoba ΜβτβπποΒ, flaianHX bmgctg c φοεφβΤΗΜΜΗ ηπη cyj ^ aTHbíMHHOH3MH TpyflHO paCTBOpHMbie OCaflKH. ΠρΗΜΗΗΟή chhxghhh taxon HaMajibHoň KOHiteHTpaiíHH b cnynae npHcyTCTBHH AOCTa-ΤΟΗΗΟΓΟ KOJIHHeCTBa εγΠΒφβΤΗΕΙΧ hohoba OKaaanCH CflBHr KHCJ1OTHOCTH CJia6OKHCnoňnHTaTejibHoň CPE / ju b CTopoHy menoMHhix 3HaneHHň pH b npouecce cTepHnH3auHH, Bbi3biBaeMbiň ocaxneHHeM CaS04 at οβη33ηημμ c sthm yuaneHHeM cpaam φΗ3Ηθπο HS-rmeCKH KHCJIbK ΟνΠΒφβΤΗΜΧ hohoba. COOTBGTCTBeHHO HaHajlbHan KOHmeHTpaUHHpacTBopHMoro ΗΠΗ 6ΗΟΠΟΓΗΗβCKH flOCTyiHiOrH K ο β τ H H H H s s s s s s s s enou s ue ue ue ue ue ue ue.. yBenmeHHe 3HaqeHHH pHoeecneHHBaeT coagaHHe CnaronpHHTHbix ycnoBHn ρ, ηκ ocaxAeHHH φοοφβτβ c onpene-neHHbMH npHcyTCTBynatHMH b nHTaTenbHoft cpefle ηοηθμη ΜβτβπποΒ. Ax ο oc A A A A A A A x x x x x x x x x x x x x pa pa pa pa pa pa pa pa pa pa----------

OcoCeHHO 6nar0npHHTHoe BAHHHHe Ha ocaxAeHne φοεφβτβ oxasbtBaeT npHMeHHeMbiňB OOAblDHHCTBe TGXHHMCCKHX φβρΜβΗΤΗΑΗή ΜβΤΟΑ ΟΤβρΗΠΗ9βΑΗΗ ΗβΠΟΟρβΑΟΤΒβΗΗΟBO3AeňcTBywiuHM BOflHHbíM napoM πόα flaBJíeHHeM, a Tarate npHcyTCTBHe CaCOj, xo-Topbiň HcnoAbsyeTGH b xauecTBe 6y4>epa απη perynHpoeaHHH pH b npouecce φβρΜβΗ*ΤβΑΗΗ.OcoCeHHO 6nar0npHHTHoe BAHHHHe Ha ocaxAeHne φοεφβτβ oxasbtBaeT npHMeHHeMbiňB OOAblDHHCTBe TGXHHMCCKHX φβρΜβΗΤΗΑΗή ΜβΤΟΑ ΟΤβρΗΠΗ9βΑΗΗ ΗβΠΟΟρβΑΟΤΒβΗΗΟBO3AeňcTBywiuHM BOflHHbíM πόα flaBJíeHHeM drinks, and tara npHcyTCTBHe CaCOj, XO-Topbiň HcnoAbsyeTGH b xauecTBe 6y4> EPA απη perynHpoeaHHH pH npouecce φβρΜβΗ b * ΤβΑΗΗ.

CoAepxaHHe φοοφβτβ b o6meM moxho CHHSHTb τβκχβ yMeHbnieHHeM aojih φοοφβτοο-Aepxanpix κομππθκοημχ cyOcTpaTOB. Tax, HanpHMep, HcnonbdyeMbift b φβρΜβΗτβΗΗΗHoypseorpHAHHa sKCTpaier coesoro rnpora couepXHT οκοπο 8 r φοεφβΤΗΟΓΟ φοοφο-pa rfa 1 r uipoTa. B nepeuHcneHHbix Bbme ycnoBHHx HananbHaH xoHueHTpauHH pacTBopeHHoro ηπη po-cTynHoro φοεφβτβ 6yueT HacTonbKo HHsxoft, hto bo bchkom cnyuaé επβΑΗφΗΜβε-ΚΗβ Α·ΠΗ HOypaeOTpHAHHOnpOAyiWPyWWHX MHKpOOpraHHSMOB BepXHHe ΠρβΑβΠΒΗΟ-ΑΟ-nycTHMbie sHaneHHH φοοφβτβ ocTaroTCH HHxe βτογο npeuena. TaxHM oOpaSoM, Ta-ΚΗβ He6oJIbniHe KOHUeHTpaUMH φοοφβτβ ΠΟ3ΒΟΠΗΙ0Τ nyTeM ΠρΗββΒΠΘΗΗΗ ΟΠρβΑβΠβΗΗΟ-ro KonHMecTBa φοοφβτβ nocne cTepHnHsauHH nonyuHTb cpeuy, b sHanHTenbHoň οτβ-neHH cTaHAapTHaoBaHHyio no φοεφβτγ. ΚροΜβ τογο, pexHM πonauη φοεφβτβ ποββοπη-βΤ ynpaBJIHTb φΟβφβΤΗΜΜ ΟβΜβΗΟΜ MHKpOOpraHHSMOB ΤΒΚΗΜ OCpaSOM, ΗΤΟβΜ MOJKHO6bl.no nonyHHTb npOAyKT C BblCOKHM BbIXOAOM, ΦοεφβΤ ΜΟΧβΤ nOAdBaTbCH B ΒΗΑβpacTBopa ηπη TBepAoro BemecTaa ηπη cycneH3HH. Φοοφβτ ΜΟχβτ npHcyrcTBOBaTtΠΗ6ο B φορΜβ CBO6OAHbIX φσοφβΤΗΒΙΧ HOHOB, ΠΗ0Ο B ΒΗΑβ ΧΗΜΗΗβΟΚΗ ΗΠΗ φΗ3ΗΗβΟΚΗcBnsaHHoro φοοφβτβ. Ilonana φοοφθτβ ηπη φοεφΒΤΟΟΑβρχβηΐΗχ BemecTB ocymecTBna-eTCH Β 33BHCHMOCTH ΟΤ peXHMa ΠΟΑβΗΗ nocne εΤβρΗΠΗ3βΑΗΗ ΗΠΗ ΒΟ ΒρβΜΗ φερΜβΗ-TaunH cnocoÓOM pasoBoro η/ηπη MHoropasoBoro η/ηπη HenpepuBHoro πρΗββΒπβΗΗπ.HenpepbIBHOe ΙίρΗθβΒΠβΗΗβ φοοφβτβ ΜΟΧβΤ OCymGCTBJIHTbCH C paanHHHOft CKOpOCTbIO.ΠΟΑβΗΗ ΠΟ ΒρβΜΗ φβρΜβΗΤβΑΗΗ. r, Β KanecTBe ποΟοπηογο ycnoBHH cneAyeT' oÓecnenHBaTb, hto6m cy6cTpaTU ηπη yc-ποβηη oKpyxaxníeň cpeflbí npH noAane φοοφβτβ, pe3ynbTHpyR»tHň H3 sToro poct ηπηnoBbnueHHan aKTHBHocTb oĎMeHa BemecTB He BbisbiBanu HeČnaronpHHTHoft πημητβαηηΑπη o6pa3OBaHHH npoAyKTa. TaKHM o6pa3OM yAaeTcn β ιΠΗρόκοή ετβπβΗΗ Hcnepnu- 258414 4 saTb enonorHHecKyio BO3Mo»HocTb HoypaeoTpHqHHooĎpaaoBaTejiH «jih obpaaoBaHHHnpoflyKTa h yBenHHHBaTb Bbixon nponyKTa. npHMepw ocymecTBJíeHHH . Η3θ6ρετβΗΗβ óonee ηο,αροδΗο noHCHHeTCfl Ha οοηοβθηηη npHMepos ocyiaecTBneHun. 1) ΙΐΗθφΗΠΗ3ΗροΒ3ΗΗΗε Ha ποΊΒβ KOHcepBHposaHHbie cnopw, ceneKTaHTa NG 13-14Hoyp3eoTpHu;nHoo6pa3yiomeFo wTaMMa Streptomyces noursei IMET JA 3890b BMcesa-mt Ha noflxoflHiuyro arapoeyio cpeňy, HanpHMep, arap OMepcona. ΠρΗΜθρκο nepeai7-flHeBHbiň nepHOfl HHKyóHpoBaHHH npH 30°C BbipeaaioT ynacTKH MHnenHanbHoro ra-3OHa paaMepoM οκοπο 2 cm2, a hmh aaceBawT KynbTypy npeflBapHTenbHoro BwpaasH-BaHHB. Čpěna npensapHTenbHoro BWpamHBaHHH conepXHT Ha oflHH πκτρ Boflonpoeofl“Hoň Bona cnenyromHe BemecTBa: 40 r rnMKO3bi, 15 r o6e3MacneHHoft ccesoň MyKH,0,3 r KH2PO4, 5 r NaCl h 3 r CaCO3. KncnoTHOcTb cpenbi ycTaHasnuBaioT nepencTepunHaanHeň Ha 3HaneHne pH 6,5. 3Ty cpeny 3anHBaioT b 3aKpwBaeMwet saTHoftπροδκοή niHpoKoropnbie 6yTbuiKH eMKocTbio 500 μπ Kaxnaa b, KonHnecTBax no 50 μπ.CTepnjnH3auHio ocymecTBnHMT 35-MHHyTHbiM HarpeBOM no 121°C b aBTOKnaBe.CoAepxaHHe φοοφβτβ b o6meM moxho CHHSHTb τβκχβ yMeHbnieHHeM aojih φοοφβτοο-Aepxanpix κομππθκοημχ cyOcTpaTOB. Tax, HanpHMep, HcnonbdyeMbift b φβρΜβΗτβΗΗΗHoypseorpHAHHa sKCTpaier coesoro rnpora couepXHT οκοπο 8 r φοεφβΤΗΟΓΟφοοφο-pa rfa 1 r uipoTa. B nepeuHcneHHbix Bbme ycnoBHHx HananbHaH xoHueHTpauHH pacTBopeHHoro ηπη after-cTynHoro φοεφβτβ 6yueT HacTonbKo HHsxoft, HTO bo bchkom cnyuaé επβΑΗφΗΜβε-ΚΗβ Α · ΠΗ HOypaeOTpHAHHOnpOAyiWPyWWHX MHKpOOpraHHSMOB BepXHHe ΠρβΑβΠΒΗΟ-ΑΟ-nycTHMbie sHaneHHH φοοφβτβ ocTaroTCH HHxe βτογο npeuena. TaxHM oOpaSoM, Ta-ΚΗβ He6oJIbniHe KOHUeHTpaUMH φοοφβτβ ΠΟ3ΒΟΠΗΙ0Τ ΠρΗββΒΠΘΗΗΗ ΟΠρβΑβΠβΗΗΟ rivet-Ro KonHMecTBa φοοφβτβ nocne cTepHnHsauHH nonyuHTb cpeuy b sHanHTenbHoň οτβ-neHH cTaHAapTHaoBaHHyio φοεφβτγ no. ΚροΜβ τογο, pexHM πonauη φοεφβτβ ποββοπη-βΤ ynpaBJIHTb φΟβφβΤΗΜΜ ΟβΜβΗΟΜ MHKpOOpraHHSMOB ΤΒΚΗΜ OCpaSOM, ΗΤΟβΜ MOJKHO6bl.no nonyHHTb npOAyKT C BblCOKHM BbIXOAOM, ΦοεφβΤ ΜΟΧβΤ nOAdBaTbCH B ΒΗΑβpacTBopa ηπη TBepAoro BemecTaa ηπη cycneH3HH. BOοοφβτ ΜΟχβτ npHcyrcTBOBaTtΠΗ6ο B φορΜβ CBO6OAHbIX φσοφβΤΗΒΙΧ HOHOB, ΠΗ0Ο B ΒΗΑβ ΧΗΜΗΗβΟΚΗ ΗΠΗ φΗ3ΗΗβΟΚΗcBnsaHHoro φοοφβτβ. Ilonana φοοφθτβ ηπη φοεφΒΤΟΟΑβρχβηΐΗχ BemecTB ocymecTBna etch Β 33BHCHMOCTH ΟΤ peXHMa ΠΟΑβΗΗ nocne εΤβρΗΠΗ3βΑΗΗ ΗΠΗ ΒΟ ΒρβΜΗ φερΜβΗ-TaunH cnocoÓOM pasoBoro η / η ηπη MHoropasoBoro / ηπη HenpepuBHoro πρΗββΒπβΗΗπ.HenpepbIBHOe ΙίρΗθβΒΠβΗΗβ φοοφβτβ ΜΟΧβΤ OCymGCTBJIHTbCH C paanHHHOft CKOpOCTbIO.ΠΟΑβΗΗ ΠΟ ΒρβΜΗ φβρΜβΗΤβΑΗΗ. r Β KanecTBe ποΟοπηογο ycnoBHH cneAyeT 'oÓecnenHBaTb, hto6m cy6cTpaTU ηπη YC-ποβηη oKpyxaxníeň cpeflbí NPH noAane φοοφβτβ, pe3ynbTHpyR »THN H3 Stora honors ηπηnoBbnueHHan aKTHBHocTb oĎMeHa BemecTB He BbisbiBanu HeČnaronpHHTHoft πημητβαηηΑπη o6pa3OBaHHH npoAyKTa. TaKHM o6pa3OM yAaeTcn β ιΠΗρόκοή ετβπβΗΗ Hcnepnu- 258414 4 saTb enonorHHecKyio BO3Mo »HocTb HoypaeoTpHqHHooDpaaBaTejiH« southBaHHnpoflyKTa h yBenHHHBaTb Bbixon nponyKTa. npHMepw ocymecTBJieHHH. Θ3θ6ρετβΗΗβ óonee ηο, αροδΗο noHCHHeTCfl Ha οοηοβθηηη npHMepos ocyiaecTBneHun. 1) πθφΙΐΗΠΗ3ΗροΒ3ΗΗΗε Ha ποΊΒβ KOHcepBHposaHHbie cnopw, cenyKTaHTa NG 13-14Hoyp3eoTpHu; nHoo6pa3yiomeFo wTaMMa Streptomyces noursei Nep y ρ-H H H H bi H H H H H H H H H H cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm p p p p p p p p Boflonpoeofl "Ho Bona cnenyromHe BemecTBa: 40 r rnMKO3bi, 15 r o6e3MacneHHoft cecho MyKH, 0.3 r KH2PO4, 5 r NaCl h 3 r CaCO3. The pH of the cell is not pH 6.5. 3Ty cpeny 3anHBaioT b 3aKpwBaeMwet saToToBoioMyToBoMoMoMpoMoToMoMoMoToMoMoMoMoMoMoMoMoMoMoMoMoMoMoMoMo.

SacesHHwe KynbTypw npesapHTenbHoro BWpamHBaHHH MHKyGHpyiOT b Teueiwe 48 *ta-cob npn 29° C Ha KananKe. lípu πομοπ$η 150 μπ nonyneHHoro τηκημ 06pa3OM noceB-ηογο MaTepHana 3aceeawT 2500 μπ ochobhoé KynbTypbi b naGopaTopHoM φερΜβΗΤβ-'ropě. flna ochobhoé KynbTypbi Hcnonb3yioT cpeny, conepwamyio b οληομ πκτρε cneňywmneHHŤaTenbHbie BemecTBa: 32 r κβρτοφεπΒΗοτο KpaxMana, 29 r γπιοκοβμ, 11 r oBea-MacneHHOň coeBOÉ MyKH, lir (NH^íSO*. 2 r MgS0<7H20, 1 r NaCl, 6 r CaCO3h 0,5 r ZnSO4· 7Hž0. Ha 2500 μπ nHTaTenbHoň cpenbi πρη6ββπημτ 1 μπ βΗΤβφροΗβb xanecTBe neHoracHTenn Ha chhhkohoboé ocnoBe. KncnoTHocTb nepen erepHnHaa-HHeň ημθθτ 3HaneHHe pH 6,0. Ilocne cTepnnHaanHH sánonHeHHoro 250 μπ KynbTy-panbHoft WHflKocTH φβρΜθΗΤΗΤορβ npH 121°C b aBTOKnaBe h nocnenywmero oxnaatfle-hhh no 30°C cTapTÓByw KHcnoTHOCTb ycTaHaBnHBaioT, ot pH 8,4 Ha pH 6,8, npH-SaBnaa acenTHnecKyM noSaBKy 1 0%-hoé CTepHnbHOÉ cepHoň κηοποτμ. 3aceHHHbieφβρΜβΗτβηκοΗΗΜβ cpenm HHKyčHpyioT b TeneHHe 168 nacoB npn TeMnepaType 3O0C hckopocth aapanHH 2500 μπ BO3nyxa b MHHyry πρκ nepeMenruBaHHH c ηηοϊομ oCopo-tob 800 o6/mHh, CnycTH 1,5 uaca nocne aacesa ochobhoé KynbTypw πρκ noMonmnepHCTanbTHnecKoro Hacoca~no3aTopa HenpepwBKO πρη6ββπημτ pácTBop 4 h KH2P04Ha ΠΗΤρ ηΗΟΤΗΠΠΗρΟΒaHHOň BOnbI Β ΤβΠβΗΗβ 5 HaCOB CO CKOpOCTbM ΠΟΠΗΗΗ1,8 Μπ/nac; ecnen 3a bthm pacTBop πρΗδβΒππΜτ b TeneHHe 21 naca co CKopoc-TbM HO3HpOBaHHH 3,5 Mn/naC. Bo ΒρβΜΗ φβρΜβΗΤβΗΗΗ Β acenTHHeCKHX ycnOBHHX tie-pHonHnecKH 6epyT npo6w KynbTypanbHOň jkhhkocth η b hhx ΜβτοηοΜ n*«l^y3HH barap c Hcnonb3OBaHHeM MHKpoopraHH3Ma Bacillus ' subtilis ATCC 6633 b KanecT-Be TecT-MHKpoopraHH3Ma οπρεηεππιοτ conepxaHHe Hóyp3eoTpHnHH—ochob3hhh, rnwKO-3 w, aMMOHHííHoro a3OTa h TBepnwx Bewec.TB. napaMeTpw ρθ£, pH h conepxaHHH C0£β οτχοηππιΗΧ H3 annapaTa ra3OB KOHTponHpywT β χοηθ nponecca ΜετοηοΜ H3Mepe-hhh c perHCTpanHeň, CHHweHHe 3HaneHHH κηοποτηοοτη KynbTypanbHoÉ khhkocthHH»e SHaueHHH pH 5,8 npenoTBpamaioT npHbaBneHHeM cTepHnhHoro 5%-ηογο pacTBO-pa enKoro Harpa. fapH CHHDKeHHH KOHneHTpanHH ΓΠΜΚΟ3Μ fíO 5 r/π ΗΠΗ aMMOHHÉHOrO a3OTa ΠΟ 0,5 r/π,hto OTpaacaeTcn Ha yBenHneHHe H3MepeHHbix 3HaneHHÉ pH η ρθ·ι» a Tarase Ha chh-xeHHH conepacaHHH C02 β οτχοπηιηηχ ra3ax, πρΗδβΒππιοτ nepnonunecKH 25 r γπιοκο-3W ΗΠΗ .10 Γ (NH4)2SO4 B BHfle KOHneHTpHpOBaHHblX CTepHHbHblX paeTBOpOB.flanbHeňniyio φερΜβΗτβηΗΜ προΒοηπτ 6ea npH6aBneHHn ΚΗ^ΡΟ^ , γπιοκο3Μ η (ψΙ^)^δθ4 258414 5 b ycnoBHHX, Me OTjiHWHbix ot oCbíMHbix. Ilocne npeKpameHHH φβρΜβΗτβΑΗΗ B KynhTy-panbHoň xhakocth npucyTCTByioT eme ηβτκο o6HapyxHBaeMbie KonnuecTBa rn»KO3bi haMMOHHH. KOHneHTpannu HOyp3eOTpHUHH-OCHOBaHHH, OnpeflejjeHHbie B (JjHJIbTpaTaXxynbTyp oCohx pasjíHMHbix <J>epMeHTaitfiOHHbix nponeccoB, πρΗΒβΑβΗΜ b TaOnHue 1 . 2) JlHO$HJiH9HpoBai«n>iň Ha iTHOKoso-xejiaraHe KOHcepBHpoBaHHbiň μμαβαηΚ (eec 6pyT-to KOHcepBHpoBaHHoro coflepwHMoro - οκοπο 300 μγ) HaaBaHHoro b npHMepe 1Hoyp3eoTpHUHHOo6pa3ywmero urraMMa ετρβπτοΜΗηβτοΒ cycneHAHpyioT c 3 μπ 0,9Z-Horo pacTBopa xnopHCTóro HaTpHfl, 0,5 μπ 3Toň cycneHSHH cnyxHT b xanectBeHHOKynioMa απη 400 μπ cpeflbí npeflBapHTenbHoro KynbTHBHpoBaHHH nepBoň CTaAHH.Cpe#a npeflBapHTenbHoň xynbTypbi ΗΜββτ τοτ xe caMbiň cocTae η KHcnoTHocTb, htob npHMepe 1, OTy cpefly 3anHBaior b 3aKptiBaeMbie eaTHOň προδκοΗ uiHpoKoropnbieCyTbinxH eMKoeTb» 2500 μπ b KonHnecTBax no 400 μπ. CTepwiHsaAHio npoBOflHT35-MHHyTHbiM HarpeBOM ao 121°C b βΒτοκπβΒβ.SacesHHwe KynbTypw npesapHTenbHoro BWpamHBaHHH MHKyGHpyiOT b Teueiwe 48 * tai-cob npn 29 ° C Ha KananKe. lime tree ο 150 μπ nonyneHHoro τηκημ 06pa3OM noceB-ηογο MaTepHana 3aceeawT 2500 μπ KobbTypbi b onGopaTopHoM φερΜβΗΤβ -rope. fln ochobhoé KynbTypbi Hcnonb3yioT cpeny, conepwamyio b οληομ πκτρε cneňywmneHHŤaTenbHbie BemecTBa: 32 r κβρτοφοτο KpaxMana, 29 r γπιοκοβμ, 11 r BBea-MacneHo CoeBOÉ MyKH, lir (NH4 SOSO *. 2 r MgS0 <7H20, 1 r NaCl, 6 r CaCO3h 0.5 ZnSO4 · r 7Hž0. Ha 2500 μπ nHTaTenbHoň cpenbi πρη6ββπημτ 1 μπ βΗΤβφροΗβb xanecTBe neHoracHTenn Ha chhhkohoboé ocnoBe. KncnoTHocTb Nepen erepHnHaa-HHeň ημθθτ 3HaneHHe pH 6.0. Ilocne cTepnnHaanHH sánonHeHHoro 250 μπ KynbTy-panbHoft WHflKocTH φβρΜθΗΤΗΤορβ NPH 121C b aBTOKnaBe nocnenywmero oxnaatfle h hhh amino-30C cTapTÓByw KHcnoTHOCTb ycTaHaBnHBaioT OT pH 8.4 pH 6.8 Ha, NPH SaBnaa acenTHnecKyM noSaBKy 1 0% -hoé CTepHnbHOÉ cepHoň κηοποτμ. 3aceHHHbieφβρΜβΗτβηκοΗΗΜβ cPen HHKyčHpyioT b TeneHHe 168 nacoB npn TeMnepaType 3O0C hckopocth aapanHH 2500 μπ BO3nyxa b MHHyry πρκ nepeMenruBaHHH c ηηοϊομ oCopo-tob 800 o6 / mHh, CnycTH 1.5 uaca nocne aacesa ob Π opa Π Π Π ob ob ob ob ob ob ob ob ob ob ob ob ob ob Ha Ha Ha Ha ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ecnen 3a bthm pacTBop πρΗδβΒππΜτ b TeneHHe 21 naca what CKopoc-TbM HO3HpOBaHHH 3.5 Mn / naC. Bo ΒρβΜΗ φβρΜβΗΤβΗΗΗ Β acenTHHeCKHX ycnOBHHX tie-pHonHnecKH 6epyT npo6w KynbTypanbHOň jkhhkocth η b HHX ΜβτοηοΜ n * «l ^ y3HH barap c Hcnonb3OBaHHeM MHKpoopraHH3Ma Bacillus" subtilis ATCC 6633 b KanecT-Be flow-MHKpoopraHH3Ma οπρεηεππιοτ conepxaHHe Hóyp3eoTpHnHH-ochob3hhh, rnwKO 3 w aMMOHHííHoro a3OTa h TBepnwx Bewec.TB. napaMeTpw ρθ £ pH £ h conepxaHHH C0 β οτχοηππιΗΧ H3 annapaTa ra3OB KOHTponHpywT β χοηθ nponecca ΜετοηοΜ H3Mepe HHH-c perHCTpanHeň, CHHweHHe 3HaneHHH κηοποτηοοτη KynbTypanbHoÉ khhkocthHH »e SHaueHHH pH5.8 npenoTBpamaioT npHbaBneHHeM cTepHnhHoro 5% -ηογο pacTBO PA Enkor Harp. fapH CHHDKeHHH KOHneHTpanHH ΓΠΜΚΟ3Μ FIO 5 r / π ΗΠΗ aMMOHHÉHOrO a3OTa ΠΟ 0.5 r / π, HTO OTpaacaeTcn Ha yBenHneHHe H3MepeHHbix 3HaneHHÉ pH η ρθ · ι »and Taras Ha CHH-xeHHH conepacaHHH C02 β οτχοπηιηηχ ra3ax, πρΗδβΒππιοτ nepnonunecKH 25 r γπιοκο- 3W ΗΠΗ .10 Γ (NH4) 2SO4 BHfle KOHneHTpHpOBaHHblX CTepHHbHblX paeTBOpOB. Ilocne npeKpameHHH φβρΜβΗτβΑΗΗ B KynhTy-panbHoň xhakocth npucyTCTByioT eme ηβτκο o6HapyxHBaeMbie KonnuecTBa rn »KO3bi haMMOHHH. KOHneHTpannu HOyp3eOTpHUHH-OCHOBaHHH, OnpeflejjeHHbie B (JjHJIbTpaTaXxynbTyp oCohx pasjíHMHbix <J> epMeHTaitfiOHHbix nponeccoB, πρΗΒβΑβΗΜ b TaOnHue 1.2) JlHO $ HJiH9HpoBai «n> In Ha iTHOKoso-xejiaraHe KOHcepBHpoBaHHbiň μμαβαηΚ (EEC 6pyT-to KOHcepBHpoBaHHoro coflepwHMoro - οκοπο 300 μγ) HaaBaHHoro b npHMepe 1Hoyp3eoTpHUHHOo6pa3ywmero urraMMa ετρβπτοΜΗηβτοΒ cycneHAHpyioT C3 μπ 0,9Z-Horo pacTBopa xnopHCTóro HaTpHfl 0.5 μπ 3ton cycneHSHH cnyxHT b xanectBeHHOKynioMa απη 400 μπ cpeflbí npeflBapHTenbHoro KynbTHBHpoBaHHH nepBoň CTaAHH.Cpe # and npeflBapHTenbHoň xynbTypbi ΗΜββτ τοτ xe caMbiň cocTae η KHcnoTHocTb, htob npHMepe 1, OTy cpefly 3anHBaior b 3aKptiBaeMbie eaTHOn προδκοΗ uiHpoKoropnbieCyTbinxH eMKoeTb »2500 μπ b KonHnecTBax no 400 μπ. CTepwiHsaAHio npoBOflHT35-MHHyTHbiM HarpeBOM and 121 ° C b βΒτοκπβΒβ.

OxnaxfleHHbie onnTb ao κομηβτηοη TeMnepaTypbi h 3ace«HHbie xynbTypbi HHKyČHpyioTb τβΗβΗΗβ 48 MacoB npH 29°C ha Kananxe (180 o6/mhh). HcnonbsyioT 1200 μπ no-nyneHHoň tbkhm oÓpasoM xynbTypanbHoň xhakocth nepeoft npenBOPHTenbHoň xynbTy-pbi, Ha BTOpoň οτάπΗκ npenBapHTenbHoro xynbTHBHpoBaHHH aaceBanr 150 πκτροΒTepMocTepnnn3OBaHHoň cpenbi (β TeneHHe 60 μηη πρκ 121°C) Taxoro xe cocTaea,xax Ha nepBoň cthahh npeABapHTenbnoro KynbTHBHpoBaHHH, conepxameftcH β φβρ-ΜβΗΤβτορβ H3 cnenHanbHoň BbicoKOKanecTBeHHofi ετβπΗ (o6xnft oÓteM - 250 πητ-poe), ocHameHHOM ycTpoňcTBaMH απη ποΑβηκ CTepunbHoro BOSAyxa η nepeMemHBaio-mHM ycTpoňcTBOM. KynbTHBnpoBaHne προβοαητ npH 27-29°C, ckopocth pa6oTW nepe-MemHBawmero ycTpoňcTBa 240 ο6/μηη h ckopocth ποαοηη BosAyxa 1 πκτρ Ha πΗτρKynbTypanbHOft xhakocth b MHHyTy. /VlH OCHOBHOň KynbTypbl MCnonbSyiOT 4>epMeHTaTOpbI H3 CneAHanbHOň BblCOKOKaHeCT“BeHHoft cTann (o6ihhH o6teM -710 πκτροβ), ocHameHHbie ycTpoňcTBaMH απη noffannCTepunbHoro BosAyxa h pacnonoxeHHbíM b ueHTpe annapaTa nepeMemHBanmHM ycTpoň-ctbom, xoTopbie sanonHeHbí 500 nHŤpaMH nHTaTenbHOň cpeAM KaxAbiň (penenTypy cm.npHMep 1). B xanecTBe neHoracHTenn ηρΗθβΒΠΗίοτ 0,5 r/n aHTa$poiia. KncnoTHOCTbycTaHaBHHBaioT nepefl cTeptuiHaaiuieň na SHaqeHHe b npenenax pH 6,7-7,0. Οτβρκ-nHsaAHio nHTaTenbHOň cpeAU (6ea KOMnoHeHTa rnioKOSbi) προβοαητ Ha MecTe npeA“BapHTenbHbíM noAorpeBOM napoM pyCaniKH npHMepHO n° 70°C h nocnenyioiUHM Ha- -rpeeoM ocTpbiM napoM ao 12t°C b TeqeHHe 15 MHHyr. Ilocne oxnaxACHHH ao τβΜΠβ-paTypbi οκοπο 30°C ηρΗββΒΠΗίοτ b acenTiwecKHX ycnoBHHX rnwKoay b BHfle 50%-ho-γο βοαηογο pacTBopa, pa3AenbHO npocTepunHaoBaHHoro 30-MHHyTHNM HarpeeoM ao121°C. ΠβρβΑ noceeoM πρπ πομοπιη cTepnnbHoft 10%-Hoň cepHoň KHcnoTbi cHHxaioTCTapTOByio KHcnoTHocTb pH 8,6 a° 3Haqe«HH B npenenax pH 6,9-7,1. .OxnaxfleHHbie onnTb and TeMnepaTypbi h 3ace «HHbie xynbTypbi HHKyČHpyioTb τβΗβΗΗβ 48 MacoB npH 29 ° C ha Kananxe (180 o6 / mhh). HcnonbsyioT 1200 μπ no-nyneHHoň tbkhm oÓpasoM xynbTypanbHoň xhakocth nepeoft npenBOPHTenbHoň xynbTy PBI-Ha BTOpoň οτάπΗκ npenBapHTenbHoro xynbTHBHpoBaHHH aaceBanr 150 πκτροΒTepMocTepnnn3OBaHHoň cpenbi (β TeneHHe 60 μηη πρκ 121 ° C) Taxoro cocTaea xe, x and x Ha nepBoň cthahh npeABapHTenbnoro KynbTHBHpoBaHHH, conepxameftcH β φβρ- 3βΗΤβτορβ H3 cnenHanbHoň BbicoKOKanecTBeHHofi ετβπΗ (o6xnft oOteM - 250 πητ-poe), ocHameHHOM ycTidedcTBaMH απη ποΑβηκ CTepunbHoro BOSAyxa η nepeMemHBaio-mHM ycTtvcTBOM. KynbTHBnpoBaHne προβοαητ npH 27-29 ° C, pa6oTW paeo pWe not-MemHBawmero ycTwo cTBa 240 ο6 / μηη h ckopocth ποαοηη BosAyxa 1 πκτρ Ha πΗτρKynbTypanbHOft xhakocth b MHHyTy. / Humid OCHOBHOň KynbTypbl MCnonbSyiOT 4> H3 epMeHTaTOpbI CneAHanbHOň BblCOKOKaHeCT "BeHHoft cTann (o6ihhH o6teM πκτροβ -710) ocHameHHbie ycTpoňcTBaMH απη noffannCTepunbHoro BosAyxa h pacnonoxeHHbíM b ueHTpe annapaTa nepeMemHBanmHM ycTpoň-ctbom, xoTopbie sanonHeHbí 500 nHŤpaMH nHTaTenbHOň CPEA KaxAbiň (penenTypy cm.npHMep 1 ). B xanecTBe neHoracHTenn ηρΗθβΒΠΗίοτ 0.5 r / n aHTa $ poiia. The THOCTbycTaHaBHHBaioT nepepl cTeptuiHaaiuin on SHaqeHHe b npenenax pH 6.7-7.0. Peτβρκ-nHsaAHio nHTaTenbHOn cpeAU (6ea KOMnoHeHTa rnioKOSbi) προβοαητ Ha MecTe npeA “BapHTenbHbiMaAppeBOM napoM pyCaniKH npHMepHO n ° 70 ° C h nocnenyioiUHM Ha-rpeeoM ocTpbiM napoM o 12t ° C b TeqeHHe 15 MHHyr. Ilocne oxnaxACHHH and β-β-paTypbi 30 ° C ηρΗββΒΠΗίοτ b acenTiwecKHX ycnoBHHX rnwKoay b BHfle 50% -h-γοβοηο pacTBopa, pa3AenbHO npocTepunHaoBaHHoro 30-MHHyTHNM HarpeeoM o121 ° C. CTepnnbHoft 10% -Hoň cepHoň KHcnoTbi cHHxaioTCTapTOByio KHcnoTHocTb pH 8.6 and 3Haqe HH B npenenax pH 6.9-7.1. .

OepMeHTanHOHHbie cpeAbi ochobhoH KynbTypbl, saceflHHbie κβχηβΗ 5Z HHOKynioMa bto-poň cTaflHH npeAsapHTenbHoro KynbTHBHpoBaHHH, KynbTHBHpywr b TeneHHe 120 narcob npH TeMnepaType b npenenax 28-30°C h co CKopocTb» nonann BosAyxa · 500 πκτροΒ b MHHyTy (AaBneHue 0,14-0,16 Míla) npH nepeMemHBaHHH (320o6/mhh). K OAHoň H3 AByx xynbTyp 'φερΜβΗτβτορβ, HannHaa co 2-ro naca H κοηηηη 32-mqacoM nocne saceea, πρη63βπηιοτ npH nOMOUw nepHCTanbTHHecKÓro Hacoca-AOsaTo-pa c nocTQHHHoň cKopocTbio BOAHbďi pacTBOp, coAepxanwň 87 r/π KH^PO4, Tax mtoβ τβπβΗΗβ 30 uacoB Ha οαηη πητρ KynbTypbl πρΗδβΒΠΗβται 40 μγ φοεφβΤΗΟΓΟ P.OepMeHTanHOHHbie cpeAbi ochobhoH KynbTypbl, saceflHHbie κβχηβΗ 5Z HHOKynioMa BTO PON cTaflHH npeAsapHTenbHoro KynbTHBHpoBaHHH, KynbTHBHpywr b TeneHHe 120 narcob NPH TeMnepaType b npenenax 28-30 ° C h as CKopocTb »nonann BosAyxa · 500 πκτροΒ b MHHyTy (0.14-0.16 AaBneHue Mile) npH nepeMemHBaHHH (320o6 / mhh). The OAHoň H3 AByx xynbTyp 'φερΜβΗτβτορβ, HannHaa CO2-ro NACA H κοηηηη 32 mqacoM nocne saceea, πρη63βπηιοτ NPH nOMOUw nepHCTanbTHHecKÓro Hacoca-AOsaTo-pa c nocTQHHHoň cKopocTbio BOAHbďi pacTBOp, coAepxanwň 87 r / π KH ^ PO4, Tax mtoβ τβπβΗΗβ 30 uacoB Ha οαηη πητρ KynbTypbl πρΗδβΒΠΗβται 40 μγ φοεφβΤΗΟΓΟ P.

Bo BpeMH φβρΜβΗΤΒΑΗΗ b acenTHHecKHX ycnoBHHX nepHOAHHecKH 6epyT npoCbi xynb-TypanbHoň xhakocth η β hhx μθτοαομ αβΦΦυ3ηη b arap c HcnojibSOBaHHeM MHKpo-opraHH3Ma Bacillus subtilís ATCC 6633 b KanecTBe TecT-MHKpoopraHHSMa οπρβΑβ-HHIOTj KOHAeHTpaAHlO HOyp3eOTpHAHH“OCHOBaHHH H COnepXaHHe rn»KO3bl, aMMOHHftHOrO 258414 6 a3OTa η TsepflbK BemecTB. flapaMeTpbi pH h conepxaHHH C02 b otxoahdihx rasaxKOHTpojiHpywr b xofle nponecca Η3ΜβρβΗΗβΜ c perHCTpanHeň. ΠρΗ na,neHHH KOHueHTpauHH rmoxosbi mane 5 r/n wm ημμοηββΚηογο asoTa hhxs0,5 r/n, ΗΤΟ BHflHO ΠΟ HapaCTaHHSO 3HaUeHHň pH H CHHXeHHW COflepMaHHH C02 B OT-xoahiuhx rasax, nepHOAHuecxH ηρΗββΒΠΗίοτ 5000 r rnwxoabi ηπη 1800 r cy.nb<t>aTaaMMOHHH B BHfle ΚΟΗΑβΗΤρΗρΟΒβΗΗΟΓΟ CTepHJIbHOro' ΒΟΑΗΟΓΟ paeTBOpa. |B Apyryw xynbTypy φβρΜβΗτβτορβ φοςφβτβ, rmoxoay ηπη ονπΒφβτβ ημμοηηη Ηβ no-6aBJiHioT. PeryriHpoBaHHe 3HaneHHH pH ne ocymecTBnneTCHj HenpepbiBHo HSMepHeMue3Η3ΗΘΗΗΗ KHCJIOTHOCTH ΠβΧβΤ B Πρββ&amp;ΠβΧ pH 6,4“7,2» B ΜΟΜβΗΤ npeKpameHHH φβρ-MeHTaijHH b xyjibTypanbHOň xhakocth copepxHTcn eme 6onee 5 r/n rnroxosbt h0,5 r/n aMMOHHňHoro ·β3οτβ.Bo BpeMH φβρΜβΗΤΒΑΗΗ b acenTHHecKHX ycnoBHHX nepHOAHHecKH 6epyT npoCbi xynb-TypanbHoň xhakocth η β HHX μθτοαομ αβΦΦυ3ηη b Arap c HcnojibSOBaHHeM MHKpo-opraHH3Ma Bacillus subtilis ATCC 6633 b KanecTBe flow-MHKpoopraHHSMa οπρβΑβ-HHIOTj KOHAeHTpaAHlO HOyp3eOTpHAHH "OCHOBaHHH H COnepXaHHe rn» KO3bl, aMMOHHftHOrO 258414 6 a3OTa η TsepflbK BemecTB. flapaMeTpbi pH h conepxaHHH C02 b otxoahdihx rasaxKOHTpojiHpywr b xofle nponecca Η3ΜβρβΗΗβΜ c perHCTpanHen. ΠρΗ on, neHHH KOHueHTpauHH rmoxosbi Mane 5 R / N WM ημμοηββΚηογο ASOT hhxs0,5 r / n, ΗΤΟ BHflHO ΠΟ HapaCTaHHSO 3HaUeHHň pH H CHHXeHHW COflepMaHHH C02 OT-B xoahiuhx rasax, nepHOAHuecxH ηρΗββΒΠΗίοτ 5000 R 1800 R rnwxoabi ηπη cy.nb <t > aTaaMMOHHH B BHfle ΚΟΗΑβΗΤρΗρΟΒβΗΗΟΓΟ CTepHJIbHOro 'ΒΟΑΗΟΓΟ paeTBOpa. | B Apyryw xynbTypes φβρΜβΗτβτορβ φοςφβτβ, rmoxoay ηνπΒφβτβ ημμοηηη Ηβ no-6aBJiHioT. PeryriHpoBaHHe 3HaneHHH pH not ocymecTBnneTCHj HenpepbiBHo HSMepHeMue3Η3ΗΘΗΗΗ KHCJIOTHOCTH ΠβΧβΤ Πρββ B &amp; ΠβΧ pH 6.4 "7.2» B ΜΟΜβΗΤ npeKpameHHH φβρ-b MeHTaijHH xyjibTypanbHOň xhakocth copepxHTcn EME 6onee 5 r / n rnroxosbt H0.5 r / n · aMMOHHňHoro β3οτβ.

KoímeHTpaixHB Hoyp3eoTpHAHH-ocHosaHHfl, onpeneneHHbie b φκπΒτρβτβχ xynbTyp o6e-thx pasntiHHbix φβρΜβΗΤβΑΗή, npHBe.fleHbí b TaSjínne 2. 3) ΠΗθφΚΠΗ3ΗρθΒβΗΗΗΗ Ha ΓΠΙΟΚΟβΟ-ΧβΠβΤΗΗβ KOHCepBHpOBaHHblft MHyennň (Bec 6pyT-to KOHcepBHpoBaHHoro coaepxHMoro - οκοπο 300 μγ) cenexTaHTa HoypseoTpHAHHo-•oOpasyiomero nrraMMa IMET JA 3890 b o6pa6aTbiBaioT b OTHomeHHH npenBapHTenbHO-ro KynbTHBHpoBaHHH, xax onncano b npHMepe 2. Λπη ocHOBHoň KynbTypw Hcnonb3yi0T naCopaTopHbiň φερΜβΗΤβτορ oSnpiM ο6βθμομ 7 π,ocHanjeHHbiň ycTpoňcTB8MH απη noflaMH cTepjunbHoro Boanyxa h pacnonoxeHHMM bneHTpe nepeMeuiHBaromHM ycTpoňcTBOM, κοτορωή aanonHHioT 4,5 π nHTaTenbHoň cpeam.KoímeHTpaixHB Hoyp3eoTpHAHH-ocHosaHHfl, onpeneneHHbie b φκπΒτρβτβχ xynbTyp o6e-THX pasntiHHbix φβρΜβΗΤβΑΗή, npHBe.fleHbí b TaSjínne 2, 3) ΠΗθφΚΠΗ3ΗρθΒβΗΗΗΗ Ha-ΓΠΙΟΚΟβΟ ΧβΠβΤΗΗβ KOHCepBHpOBaHHblft MHyennň (BEC 6pyT-to KOHcepBHpoBaHHoro coaepxHMoro - οκοπο 300 μγ) cenexTaHTa HoypseoTpHAHHo- • oOpasyiomero nrraMMa IMET JA 3890 b b o6pa6aTbiBaioT OTHomeHHH npenBapHTenbHO-ro KynbTHBHpoBaHHH, XAX onncano b npHMepe second Λπη ocHOBHoň KynbTypw Hcnonb3yi0T naCopaTopHbiň φερΜβΗΤβτορ oSnpiM ο6βθμομ 7 π, ocHanjeHHbiň ycTpoňcTB8MH απη noflaMH cTepjunbHoro Boanyxa h pacnonoxeHHMM bneHTpe nepeMeuiHBaromHM ycTpoňcTBOM, κοτορωή aanonHHioT 4.5 π nHTaTenbHoň CPEA.

KyAbTHBHpOBaHHe ΠΡΟΒΟΑΗΤ B ΜΟΑΗφΗΑΗρΟΒβΗΗΟΰ Cpefle (npHMep OCynteCTBneHHH I),coAepxameň 60 r/n rni0KO3bi. B xanecTBe neHoracHTenn πρκδβΒΠΗίοτ 0,5 r aHTa-φροΗβ NM-40 Ha ηκτρ KynbTypanbHoň xaakocth. βΠΗ MOnejlHpOBaHHH ΤΘΧΗΗΗβΟΚΗΧ yCHOBHň CTepHJIH3aAHlO ΠΡΟΒΟΑΗΤ B φβρΜβΗΤβΤΟρβH3 cneAnanbHoň BbicoxoxaHecTBeHHoň CTanH, sanonHeHHOM 2500 n nHTaTenbHoň cpe-Abi 6β3 γπιοκοβηογο KOMnoHeHTa. KHcnoTHOCTb ycTaHHBnuBaioT nepen CTepKAHaaimeňHa pH 7,4. CTepnnH3auHio προβοαητ Ha MecTe npeABapHTenbfibíM noAorpeeoM napoM.py6amxH po 75° C h nocnenyromHM HarpeBOM oerpbiM napoM no 120°C b τβΊβΗΗβ15 MHHyT·· Ilocnei oxnaxneHHH no 29°C npuCaBnHioT b acenTHuecxHX ycnoBHHX rnto-KO3y, paanenbHo npocTepHHH30BaHHyto b BHAe 50%-ηογο pacTsopa 30-MHHyTHhiM Ha-rpeBOM po 120°C. nepen aaceBOM ποΗΗΧβιοτ cTapTOByw KHcnoTHOCTb ot 3HaqeHHflpH 8,1 cepHoň KHcnoToň thkhm oOpasoM, hto6h cTapTOBoe SHaneHne pH cocTaenH-no nocne sacesa 7,4. OepMeHTanHOHHyio xynbTypy 3aceBaioT 4% HHoxynioMa BTopoftcTanHH npenBapHTenbHoro KynbTHBHpoBaHHH. flnn npoBeneHHH οπωτβ H3 φβρΜβΗτβ-τορβ nepeBOA«T 4,5 ň cpenbi b naSopaTopHbiň φβρΜβΗτβτορ, xoTopbiň CTepunnsyioTpaaflenbuo b He3anonneHHOM coctohhhh b aBTOKnaee.KyAbTHBHpOBaHHe ΠΡΟΒΟΑΗΤ B ΜΟΑΗφΗΑΗρΟΒβΗΗΟΰ Cpefle (npHMep OCynteCTBneHHH I), coAepxameň 60 r / n rni0KO3bi. B xanecTBe neHoracHTenn πρκδβΒΠΗίοτ 0.5 r aHTa-φροΗβ NM-40 Ha ηκτρ KynbTypanbHoň xaakocth. βΠΗ MOnejlHpOBaHHH ΤΘΧΗΗΗβΟΚΗΧ yCHOBH CT CTepHJIH3aAHlO ΠΡΟΒΟΑΗΤ B φβρΜβΗΤβΤΟρβH3 cneAnanbHoňn BbicoxoxaHecTBeHHoon CTanH, sanonHeHHOM 2500 n nHTaTenbHoň cpe-Abi 6β3 γπιοκοβηογο KOMnoHeHTa. KHcnoTHOCTb ycTaHHBnuBaioT nepen CTepKAHaaimeHa pH 7.4. CTepnnH3auHio προβοαητ Ha MECT npeABapHTenbfibíM noAorpeeoM napoM.py6amxH to 75 ° C h nocnenyromHM HarpeBOM oerpbiM drinks but 120 DEG C. b τβΊβΗΗβ15 MHHyT ·· Ilocnei oxnaxneHHH No 29 C b npuCaBnHioT acenTHuecxHX ycnoBHHX RNTO-KO3y, paanenbHo npocTepHHH30BaHHyto b BHAe 50% -ηογο pacTsopa 30-MHH-thiMH-rpeBOM at 120 ° C. non-aceBOM ποΗΗΧβιοτ cTapTOByw KHcnoTHOCTb ot 3HaqeHHflpH 8.1 cepHoN KHcnoToň thkhm oOpasoM, hto6h cTapTOBoe SHaneHne pH cocTaenH-no nocne sacesa 7,4. OepMeHTanHOHHyio xynbTypes 3aceBaioT 4% HHoxynioMa BTopoftcTanHH npenBapHTenbHoro KynbTHBHpoBaHHH. flnn npoBeneHHH οπωτβ H3 φβρΜβΗτβ-τορβ nepeBOA T T 4,5 c cpenbi b at SopaTopHbi φβρΜβΗτβτορ, xoTopbien CTepunnsyioTpaaflenbuo b He3anonneHHOM coctohhhh b aBTOKnaee.

OcHOBHyto xynbTypy BbipamHBatoT b TeneHHe 146 naco-B npH TeMnepaType 29°C h cko-pocTH aapauHH 4,5 n BO3nyxa b MHHyTy. Hauanbiioe uncno οδοροτοΒ nepeMeuiHBaro-ntero ycTpoňcTBa b 800 o6/mhh yBenHHHBaioT pnu ynymneHHH cHaSxeHHH xynbTypbiKHcnopOAOM; ηηηηηηη c 28-ro naca hhcao οδοροτοΒ MeiuanxH cocTaBnaeT1000 o6/mhh. Mexny 2-m h 40-m nacaMH nocne 3aceBa npH πομοπιη nepHCTanbTH-Hecxoro Hacoca πρκββΒΠΗίοτ 784 μγ KH2P04 b BHAe βοαηογο pacTBopa c paaHoňcxopocTbH) nonanH b npenenax 1,25-1,75 μγ φοοφβΤΗΟΓο P b uac h Ha πητρ Kynb-TypanbHoň xhakocth. ABTOMaTHnecxoň nonaneň 25Ζ-ηογο βοαηογο pacTBopa aMMHa—xa o6ecneHHBaioT rBOnnepxaHHe perynHpyeMoro 3HaneHHH Ha ypoBHe He Meóee pH6,2 η, τβΜ caMbiM, AocTaTOHHoe cHá6xeHHe xynbTypbi aMMOHHňHbíM a3OTOM. Γπιοκο-ay πρΗββΒΠΗίοτ nepHOAHHecxH b BHne KOHueHTpHpoBaHHoro βοαηογο pacTBopa Bcer-pa Torna, Korná pe3ynbTaTbi aHannsoe, npoBOHHMbix no xony φερΜβΗΤβΑΗΗ, noxa-3biBawT naneHHe κοηαθητρβαηη rmoxosbi HHxe 10 r/n. Bo epeMH φβρΜθΗΤβηΗΗ b 258414 7 acerrrHHecKHx ycnosHHx nepnoxumecKn 6epyT προ6ω xynbTypanbHoft khxxkocth h b«frwibTpaTax KynbTypw οπρβχχεηΗΐοτ KOHyeHTpawuo Hoyp3eoTpniínH—ocHOBaHHH Μετο/χοΜΑΗφφγ3ΗΗ b arap (TaCnHixa 3). 4) B npoixecce φερΜεκτβιχηΗ, npoBOflHMOM aHanornnHO npHMepy ocymecTBJíeHHH 3),fíonn cynbýaTa nHHxa cpeflbí ochobhoíí xynbTypbi aaMeHjnoT Ha 0,2 r/n FejíSO,,)^ *9 HjO. KoHixeHTpaixHH HoypaeoTpHiXHHa b φΗηητρβτε xynbTypbi cocTaBJíHeT nocne 120-uacoBoň φερΜβΗτβιχΗΗ 8200 μκγ/mji (TaGnnxxa 4). 5) B npoixecce φερΜβΗτειχΗΗ, προβοαημομ aHanornwHO npHMepy ocymecTBneHHH 3),HipHHHtoynynynynynambbbb byenebbe 146bbbbbbH Te 146 146epa 146 146 146 146 146-146 146 146 146 146 146 146 146MMMMMMMMMMMMMM ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° °. Hauanbiioe uncno οδοροτοΒ nepeMeuiHBaro-ntero ycTm3cBb b 800 o6 / mhh yBenHHHBaioT pnu ynymneHHH cHaSxeHHH xynbTypbiKHcnopOAOM; ηηηηηηη c 28-ro naca hhcao οδοροτοΒ MeiuanxH cocTaBnaeT1000 o6 / mhh. 2-m h 40-m nacaMH nocne 3aceBa npH 784 μg KH2P04 b BHAe βοαηογο pacTBopa c paaHoňcxopocTbH nonanH b npenenax 1,25-1,75 μγ φοοφβΤΗΟΓο P b uac h Ha πητρ Kynb-TypanbHoň xhakocth. ABTOMaTHnecxon nonaneň 25Ζ-ηογο βοαηογο pacTBopa aMMHa — xa6xneHHBaioT rBOnnepxaHHe perynHpyeMoro 3HaneHHH Ha ypoBHe He Meoee pH6,2 η, τβΜ caMbiM, AocTaTOHHoe cH6xeHHe xynbTypbi andMMOHHbHM x3TOM. HOπιοκο-ay πρΗββΒΠΗίοτ nepHOAHHecxH b BHne KOHueHTpHpoBaHHoro βοαηογο pacTBopa Bcer-pa Torna, Korna pe3ynbTaTbi and Hannsoe, npoBOHHMbix no xony φερΜβΗΤβΑΗΗ, noxa-3biBawT naneHHe κοηαθητρβαηη rmoxosbi HHxe 10 r / n. Bo epeMH φβρΜθΗΤβηΗΗ b 258414 7 acerrrHHecKHx ycnosHHx nenoxumecKn 6epyT προ6ω xynbTypanbHoft khxxkocth h b «frwibTpaTax KynbTypw KOHyeHTpawuo Hoyp3eoTpniinH — ocHOBaHHH /ετο / χοΜΑΗφφγ3ΗΗ b arap (TaCnHixa 3). 4) B npoixecce φερΜεκτβιχηΗ, npoBOflHMOM and ornnnnnnnnnpHMepy ocByecnHHH 3), cynblytin and nHHxa cpeflbi is xynbTypbi aMeHjnoT Ha 0.2 r / n Fe 1 SO 4 · 9 9 H 10. KoHixeHTpaixHH HoypaeoTpHiXHHa b φΗηητρβτε xynbTypbi cocTaBJíHeT nocne 120-uacoBoň φερΜβΗτβιχΗΗ 8200 μκγ / mji (TaGnnxxa 4). 5) B npoixecce φερΜβΗτειχΗΗ, προβοαημομ aHanornwHO npHMepy ocymecTBneHHH 3)

flOJiío cynbftaTa ιχηηκβ aaMenjnoT Ha 0,13 r/n A12(SO4) 18 H20. KoHtxeHTpaixHRflNiCe cAnBftaTa ιχηηκβ aaMenjnoT Ha 0.13 r / n A12 (SO4) 18 H 2 O. KoHtxeHTpaixHR

HoypaeOTpHUHHa b φπηΒτρβτβ xyjibTypbi cocTaBJíaeT nocne' 120-nacoBoň φερΜβΗτβ-ixhh 8400 μχγ/μπ (TaSjíHna 4). 6) B flajibHeftmeň φβρΜβΗτβιχΗΗ, b 3HanHTenbHoň CTenenu npoBORHMoft aHanoruHHOnpHMepy ocymecTBJíeHHH 5), xonHwecTBO coflepxanxerocn b nHTaTenbHoň cpene o6ea-MacjienHoro coeBoro nipoTa CHHxatoT ot 11 r/n ρ,ο 4 r/n» flanee oHaneHHe pH cpenuycTaHaBnusaioT nepea cTepHnH3aixHeň pa36aBneiiHUM boahmm pacTBOpoM enxoro Har-pa Ha pH 8,1« Ilocne cTepunHaaxxHH napoM non naeneHHeM 3HaueHHc pIlycTaHaertH“aaioT KOHneHTpHpOBaHHoň cepHoň xHcnoToň TaxHM o6pa3OM, htoGm oho cocraBnanopH 7,2 nocne sacéBa. Φοοφβτ npn6aBnHtoT Mexny 2-m h 72-m nacaMH πρκ πομοπχηβοηηογο pacTBopa KH2P0^, xoTopwň ΠρΗββΒΠΗΙΟΤ C nOCTOHHHOň CXOpOCTb» T8XHM o6-paaoM, HToCbi Gbino oGecneneHO cHaGxeHHe φοοφβτοΜ b xonHnecTBe 1,4 μγ φοεφβτ-ηογο P b sac h Ha πητρ xynbTypanbHoň jkhxxxocth. B npoixecce φβρΜβΗΤΗΐχΗΗ rnioxo-ay nepHOAHnecKH ηρΗββΒηΗΐοτ tbkhm o6pa3OM, htoGm χοιπχεΗτ pannu γπιοκο3μ He chh-3HJiaCb HHXe 1 r/n. 3ΗβΊβΗΗ6 pH nOXmepMHBaiOT ΤΗΤρΟΒβΗΗβΜ 25%-HMM pacTBOpoMTHAPOOKMCH HMMOHHHHaypOBHe pH 6,0. IIonynaeMbie χοΗηβπτρβιχΗΗ HoypaeoTpHixHHa npHBenenbi b TaGnHixe 5. 1HoypaeOTpHUHHa b φπηΒτρβτβ xyjibTypbi cocTaBJíaeT nocne '120-nacoBoň φερΜβΗτβ-ixhh 8400 μχγ / μπ (TaSjíHna 4). 6) B flajibHeftmeň φβρΜβΗτβιχΗΗ b 3HanHTenbHoň CTenenu npoBORHMoft aHanoruHHOnpHMepy ocymecTBJíeHHH 5) xonHwecTBO coflepxanxerocn b nHTaTenbHoň cPen o6ea-MacjienHoro coeBoro nipoTa CHHxatoT rt 11 r / n ρ, ο 4 r / n »flanee oHaneHHe pH cpenuycTaHaBnusaioT NEP cTepHnH3aixHeň pa36aBneiiHUM boahmm pacTBOpoM enxoro Har -pa Ha pH 8.1 Ilocal cHepcHaaxxHH for non-naphthaHHeM 3HaueHHc pIlycTaHaertH "and thioTHeHHePoPeNHeCoNHeNHyxHoOn TaxHM o6pa3OM, htoGm oho cocraBnanopH 7,2 nocne suaBa. Φοοφβτ npn6aBnHtoT Mexny m-2 h-72 m nacaMH πρκ πομοπχηβοηηογο pacTBopa KH2P0 ^, C xoTopwň ΠρΗββΒΠΗΙΟΤ nOCTOHHHOň CXOpOCTb »T8XHM O6-paaoM, HToCbi Gbino oGecneneHO cHaGxeHHe φοοφβτοΜ b xonHnecTBe μγ φοεφβτ 1,4-b ηογο P h SAC Ha πητρ xynbTypanbHoň jkhxxxocth. B npoixecce φβρΜβΗΤΗΐχΗΗ rnioxo-ay nepHOAHnecKH ηρΗββΒηΗΐοτ tbkhm o6pa3OM, htoGm χοιπχεΗτ pannu γπιοκο3μ He chh-3HJiaCb HHXe 1 r / n. 3ΗβΊβΗΗ6 pH nOXmepMHBaiOT ΤΗΤρΟΒβΗΗβΜ 25% -HMM pacTBOpoMTHAPOOKMCH HMMOHHHaypOBHe pH 6.0. IIonynaeMbie χοΗηβπτρβιχΗΗ HoypaeoTpHixHHa npHBenenbi b TaGnHixe 5. 1

0opMyna HaoGpeTeHHH0opMyna HaoGpeTeHHH

CnocoG φβρΜβΗΤβτΗΒΗΟΓΟ nonyneHHB HoypaeoTpHixHHa nyTeM ycTaHOBXH χοηιχβΗΤρβιχΗΗOHonornnecKH flOCTynHoro φοοφβτβ, oTnHuátonxHňcn τ e μ, ητο Mepó-πρΗΗΤΗΗ ηρΗΗΗΜβιοτ B OTflenbHOCTH Hnu b χομΘηηβιχηη TaKHM oGpaaoM, hto - CTepHnuaanH»'nHTaTenbHbix cpen προΒο/χητ βοαηημμ napoM non naaneHHeM bnpMcyTCTBHH hohob MeTannoB, oGpaayioaxHX c φοοφβτοΜ τργχχΗσ pacTBopHMweocaflKH, nocne npenmecTByiomeň ycTaHOBXH xhchothocth ηητβτβηΒΗωχ cpen HasHaneHHfl pH 7 - pH 9 h/ioth onHOBpeMeHHoro npHcyrcTBHS hohob cyinMfcaTa hxapGoHaTa xanbixHH, - coflepxaHHe φοεφΒτεοχχβρχβηχΗΧ xoMnnexcHbK cyGcTpaTOB b nHTarenbHwx cpeftaxcHHxatoT nepen cTepHnHaanneň, - nnn npenoTBpameHHH n&amp;nbHeňniHx ηκΜΗΤβιχΗΗ φοοφβτ πρκββΒηηιοτ b HananeφΗΠΒΤρβΧΧΗΗ Η/ΗΠΗ BO ΒρβΜΗ flanbHeňlUeň φβρΜβΗΤΒΧΧΗΗ. K Heft τβθηΗίχω Ha 5-η cTpaHnqax 258414 8CnocoG φβρΜβΗΤβτΗΒΗΟΓΟ nonyneHHB HoypaeoTpHixHHa rivet ycTaHOBXH χοηιχβΗΤρβιχΗΗOHonornnecKH flOCTynHoro φοοφβτβ, oTnHuátonxHňcn τ e μ, ητο MEPO-πρΗΗΤΗΗ ηρΗΗΗΜβιοτ B OTflenbHOCTH HNU b χομΘηηβιχηη Takhte oGpaaoM, HTO - CTepHnuaanH » 'nHTaTenbHbix cPen προΒο / χητ βοαηημμ beverages non naaneHHeM bnpMcyTCTBHH hohoba MeTannoB, oGpaayioaxHX c φοοφβτοΜ τργχχΗσ pacTBopHMweocaflKH, night npenmecTByiomeň ycTaHOBXH xhchothocth ηητβτβηΒΗωχ cPen HasHaneHHfl pH 7 - pH 9 h / IOTH onHOBpeMeHHoro npHcyrcTBHS hohoba cyinMfcaTa hxapGoHaTa xanbixHH - coflepxaHHe φοεφΒτεοχχβρχβηχΗΧ xoMnnexcHbK cyGcTpaTOB b nHTarenbHwx cpeftaxcHHxatoT Nepen cTepHnHaanneň - NNN npenoTBpameHHH n &amp; nbHeňniHx ηκΜΗΤβιχΗΗ φοοφβτ πρκββΒηηιοτ b HananeφΗΠΒΤρβΧΧΗΗ Η / ΗΠΗ BO ΒρβΜΗ flanbHeňlUeň φβρΜβΗΤΒΧΧΗΗ. K Heft τβθηΗίχω Ha 5-η cTpaHnqax 258414 8

Ta6ji«na 1 ·Ta6ji «to 1 ·

CoaepxaHHe HoypaeoTpinjHHa b KynbTypaxuiTaMMa Streptomyces noursei IMER JA 3890b--//NG 13-14 c floSaBKaMH KH^PO^ rn»KO3M h(NH4)7804 h 6ea noGasoK b JiaCopaTopHOMφβρΜβΗΤβΤΟρβ B 3a8HCHMOCTH OT ΒρβΜβΗΗφβρΜβΗΤΗΗΗΗCoaepxaHHe HoypaeoTpinjHHa b KynbTypaxuiTaMMa Streptomyces noursei IMER JA 3890b - // NG 13-14 c floSaBKaMH KH ^ PO ^ rn »

BpeMfl φβρΜΘΗ- C floSaBKaMH Ses AO0aBOK (KOMTpOJIb) , TaUHH, M μκγ/μπ 1 2 μκγ/μπ 1 v 24 1035 249 415 100 48 2980 165 1805 100 72 5412 294 1840 100 96 8311 213 3905 100 120 8800 195 4520 100 140 9230 213 4005 100BpeMfl φβρΜΘΗ- C floSaBKaMH es A K K T OJ 2 2 2 2 1 2 μκγ / μπ 1 v 24 1035 249 415 100 48 2980 165 1805 100 72 5412 294 1840 100 96 8311 213 3905 100 120 8800 195 4520 100 140 9230 213 4005 100

Ta6nuua 2:Ta6nuua 2:

Co/xepxaHHe Hoyp3eoTpHUHHa b KynbTypaxniTaMMa Streptomyces noursei IMET JA 3890b--//NG 13-14 c fíoSaBKaMH KH2P04, rntoKOStí h(NH4)2S04 h 6ea floGaBOK β φβρΜβΗτβτορβ eM-KOCTblO 500 Π B aaBHCHMOCTH OT ΒρβΜβΗΗφβρΜβΗΤβμΗΗCo / xepxaHHe Hoyp3eoTpHUHHa b KynbTypaxniTaMMa Streptomyces noursei IMET JA 3890b - // NG 13-14 c fiSaBKaMH KH2P04, rntoKOSt h (NH4) 2S04 h 6ea floGaBOK β φβρΜβΗτβτορβ eM-KOCTblO 500 Π B aaBHCHMOCTH OT ΒρβΜβΗΗφβρΜβΗΤβμΗΗ

BpeMfl φερΜβΗ-TaUHH, fl C AoCaBKaMH Bea floóasoK (κοητροπβ). μκγ/μπ z μΚγ/ji 1 Z 20 225 216 104 100 44 1600" 228 702 100 68 4858 299 1627 100 92 4903 208 2362 100 1 16 6862 191 3585 100 144 7781 200 3885 100.BpeMfl εερΜβΗ-TaUHH, fl C AoCaBKaMH Bea flooasoK (κοητροπβ). μκγ / μπ z μΚγ / ji 1 Z 20 225 216 104 100 44 1600 "228 702 100 68 4858 299 1627 100 92 4903 208 2362 100 1 16 6862 191 3585 100 144 7781 200 3885 100.

Ta&amp;mma 3:Ta &amp; mma 3:

CoflepxaHHe HoypaeoTpmjHHab KyjibType ceneKTaHTanrraMMa Streptomyces nour-sei IMET JA 3890b c no&amp;aB-K3MH ΚΗζΡ04> ΓΠ10ΚΟ3Μ H(NH+)2S04 β φβρΜεΗτβτορβeMKOCTb» 4,5 π b 3aBHcn-<MOCTH OT ΒρβΜβΗΗ φερΜβΗ-TayHHCoflepxaHHe HoypaeoTpmjHHab KyjibType ceneKTaHTanrraMMa Streptomyces nour-sei IMET JA 3890b c no &amp; aB-K3MH ΚΗζΡ04> ΚΟΠ10ΚΟ3Μ H (NH +) 2S04 β φβρΜεΗτβτορβeMKOCTb »4,5 π b 3aBHcn- <MOCTH OT ΒρβΜβΗΗ φερΜβΗ-TayHH

BpeMfl φερΜβΗτβμΗΗ,q KoHueHTpanHH Hoyp-seoTpHUHHa, μκγ/μπ 1 1 20 320 44 2330 258414 9 ΠροηοπχβΗΗβ TaOji.3 _ i __ ......Ί_________?________ 68 6240 92 8010 116 11040 146 14070BpeMfl φερΜβΗτβμΗΗ, q KoHueHTpanHH Hoyp-seoTpHUHHa, μκγ / μπ 1 1 20 320 44 2330 258414 9 ΠροηοπχβΗΗβ TaOji.3 _ i __ ...... Ί _________? ________ 68 6240 92 8010 116 11040 146 14070

Ta6jiHqa 4:Ta6jiHqa 4:

CoflepxaHHe Hoyp3eoTpHHHHanpoBeaeHHoň φερΜεΗτβίφΗ bCOOTBeTCTBHH C ΠρΗΜβρΟΜocymecTBjíeHHH 4) c ηοηημηxene3a (XII) η φερΜβΗτβΐίΗΗB COOTBeTCTBHH C ΠρΗΜβρΟΜocymecTBjíeHHH 5) c ηοηβμηaniOMHHHH (III) B 3HBHCH“MOCTH OT BpeMeHH φβρΜβΗΤβ“UHHCoflepxaHHe Hoyp3eoTpHHHHanpoBeaeHHoň φερΜεΗτβίφΗ bCOOTBeTCTBHH ΠρΗΜβρΟΜocymecTBjíeHHH C 4) C ηοηημηxene3a (XII) η C φερΜβΗτβΐίΗΗB COOTBeTCTBHH ΠρΗΜβρΟΜocymecTBjíeHHH 5) c ηοηβμηaniOMHHHH (III) B 3HBHCH "OT MOCTH BpeMeHH φβρΜβΗΤβ" UHH

BpeMH φβρΜβΗ-TaUHH, M KoHqeHTpaijHH HoypaeoTpH-UHna b μκγ/mji no* npHMepyocymecŤBneHHH A) ] 5) 24 530 800 48 2500 3200 72 .5400 6300 96 7250 7400 120 8200 8400BpeMH φβρΜβΗ-TaUHH, M KoHqeHTpaijHH HoypaeoTpH-UHna b μκγ / mji no * npHMepyocymecbneHHH A)] 5) 24 530 800 48 2500 3200 72 .5400 6300 96 7250 7400 120 8200 8400

TaCnnua 5:TaCnnua 5:

CoflepjKaHHe Hoyp3eoTpnuHHaB KyjIbTypajIbHOH WHflKOCTHnaČopaTopHoro φερΜβΗτβτορΒc flo6aBKaMH ΚΗ2Ρ04, γπιοκο-3bl H THflpOOKHCH aMMOHHH,coflepxaujeň ηοηη λπιομηηηη(III), B aaBHCHMOCTH OTBpeMeHH φερΜβΗτβμΗΗCoflepjKaHHe Hoyp3eoTpnuHHaB KyjIbTypajIbHOH WHflKOCTHnaČopaTopHoro φερΜβΗτβτορΒc flo6aBKaMH ΚΗ2Ρ04, γπιοκο 3BL-H THflpOOKHCH aMMOHHH, coflepxaujeň ηοηη λπιομηηηη (III), B aaBHCHMOCTH OTBpeMeHH φερΜβΗτβμΗΗ

BpeMH φβρ- KoHqeHTpayHH HoypaeoTpHUH-MeHTauHH,H Ha, μκγ/μη 24 800 48 . . · 2000 72 5660 96 8900 • 120 11000 258414 10 ΑηΗΟΤΒΑΗΠBpeMH φβρ- KoHqeHTpayHH HoypaeoTpHUH-MeHTauHH, H Ha, μκγ / μη 24 800 48. . · 2000 72 5660 96 8900 • 120 11000 258414 10 ΑηΗΟΤΒΑΗΠ

HaoSpeTeHHe othochtch k nojiyqeHHio οτρθπτοτρηαηηοβογο 8Ητη6ηοτηκ3 HoypseoT-pniyuia ΜΗκροδΗοποΓΗΗβοκοίί (fcepMeHTaimeň πγτβΜ οκ33ΒΗηη BosfleňcTBHH Ha φοοφβτ-huA οβΜβΗ, Pfin 9τοή μβπκ ocymecTBnnioT flBe MaHHnyjiHUHH OflHy aa Apyroft κπηpa3A&amp;ni>Ho: - cHHKeHHe HaqanbHoň κοΗΑβΗτρβΑΗΗ pacTBopuMoro h AocTyraoro φοεφβτβ ao hhs-Koro ypoBHa bo BpeMH CTepiuiHsaAHH nHTaTenbHoií cpeAbi * ' ’ » » - noBTopHoe npH.6aBjieHHe φοοφβτβ nyTeM ποΑβΗΗ φοεφβτεοΑβρχβπΐΗχ BentecTB b ποα-xoAHoteM peKHMe αοβηροβηηηη»HaoSpeTeHHe othochtch to nojiyqeHHio οτρθπτοτρηαηηοβογο 8Ητη6ηοτηκ3 HoypseoT-pniyuia ΜΗκροδΗοποΓΗΗβοκοίί (fcepMeHTaimeň πγτβΜ οκ33ΒΗηη BosfleňcTBHH Ha φοοφβτ-hua οβΜβΗ, Pfin 9τοή μβπκ ocymecTBnnioT flBe MaHHnyjiHUHH OflHy aa Apyroft κπηpa3A &amp; Her> Ho - cHHKeHHe HaqanbHoň κοΗΑβΗτρβΑΗΗ pacTBopuMoro h AocTyraoro φοεφβτβ and HHS-Koro ypoBHa bo BpeMH CTepiuiHsaAHH nHTaTenbHoií cpeAbi * '' »» - noBTopHoe npH.6aBjieHHe φοοφβτβ nyTeM ποΑβΗΗ φοεφβτεοΑβρχβπΐΗχ BentecTB b ποα-xoAHoteM peKHMe αοβηροβηηηη »

CmcKeHne liauajibMoň KOHpeHTpamut AOCTynHoro φοοφβτβ προβοαητ nyreM yBenHHe-hhh 3naMeHHH pH bo BpeMH cTepunnsanHH η ocaxAeHHH φοοφβτοοηβρχβπΒίχ ocanKOBB npncyTCTBHH φόοφβΤΟΟβΧΑβΙΟΙίρΐΧ HOHOB. KHCJIOTHOCTb φερΜβΗΤΗΑΗΟΗΗΜΧ cpefl jih6oycTaHaBJíHBaioT bo BpeMH cTepnnn3annn npHMo Ha niejioHHbie SHaueHHH pH, λη6ο yae-AHHHBaioT aa cm6t npncyTCTBHH 6onee BbicoKHX KOHineHTpaAHň εγπΗφβτβ HapHAy CKapCoHaTOM xanbAHH bo BpeMH cTepunnaainHH ocTpbiM napoM, floSaBjíeHHe φοοφβτβ bφοοφΒτοοΑβρχβπξΗΚ BewecTBax προβοαητ no onTHManbHOMy pexHMy bo BpeMH φβρΜβΗ-TaimH c npeAOTBpameHHeM AajibHeňoiHx πημηΤ8αη6 ηπη αρυγηχ HeCnaronpHHTHMX yc“ JIOBHft. ΠρΗ3Η3Η0 Η3θ6ρ©ΤβΗΗβΜ ΠΟ peayjIbTaTaM 3KCnepTH3bl, OCymeCTBfieHHOft BeAOMCTBOMno AenaM Η3θ6ρβτβΗΗή h naTeHTOB TflP. - 258414CmcKeHne liauajibMoň KOHpeHTpamut AOCTynHoro φοοφβτβ προβοαητ nyreM yBenHHe-hhh 3naMeHHH pH bo BpeMH cTepunnsanHH η ocaxAeHHH φοοφβτοοηβρχβπΒίχ ocanKOBB npncyTCTBHH φόοφβΤΟΟβΧΑβΙΟΙίρΐΧ HOHOB. KHCJIOTHOCTb φερΜβΗΤΗΑΗΟΗΗΜΧ cpefl jih6oycTaHaBJíHBaioT bo BpeMH cTepnnn3annn npHMo Ha niejioHHbie SHaueHHH pH λη6ο Yae-AHHHBaioT aa cm6t npncyTCTBHH 6onee BbicoKHX KOHineHTpaAHň εγπΗφβτβ HapHAy CKapCoHaTOM xanbAHH bo BpeMH cTepunnaainHH ocTpbiM beverages floSaBjíeHHe φοοφβτβ bφοοφΒτοοΑβρχβπξΗΚ BewecTBax προβοαητ well onTHManbHOMy pexHMy bo BpeMH φβρΜβΗ-TaimH c npeAOTBpameHHeM AajibHeňoiHx πημηΤ8αη6 ηπη αρυγηχ HeCnaronpHHTHMX yc “JIOBHft. Πρθ3Η3Η0 Η3θ6ρ © ΗΗβΗΗβΜ ΠΟ peayjIbTaTaM 3KCnepTH3bl, OCymeCTBfieHHOft BeAOMCTBOMno AenaM Η3θ6ρβτβΗΗή h naTeHTOB TflP. - 258414

Claims (1)

PŘEDMĚT VYNÁLEZU Způsob fermentační výroby nourseotricinu upravenímkonoentraoe biologicky dostupného fosfátu, vyznačující setím, že se opatření používají odděleně nebo ν' kombinaci takže - sterilizace živně půdy se provádí pod tlakem vodníparou v přítomnosti iontů kovů vytvářejících s fosfátemtěžko rozpustné sraženiny, po předběžném upravení kyselostiživné půdy na hodnotu pH 7 až pH 9 a/nebo současně přítom-ností iontů sulfátu a uhličitanu vápenatého, - obsah kom-plexních substrátů obsahujících fosfát v živných půdách sepřed sterilizací sníží a pro zabránění jeho dalšího sníženíse sulfát přidává na počátku fermentace a/nebo po dobu .další fermentace. Uzgorodský výrobne-poligrafický podnik, Proektnaja 4, Užgorod» 6060, Cena“ 2,40 KčsOBJECT OF THE INVENTION A method of fermenting the production of nourseotricin by treating a bioavailable phosphate concentrate, wherein the measures are used separately or in combination so that the sterilization of the soil nutrient is carried out under water pressure in the presence of metal ions forming poorly soluble precipitates, after pre-treatment of the acidifying soil with pH 7 to pH 9 and / or at the same time the presence of sulphate and calcium carbonate ions, the content of complex phosphate-containing substrates in the nutrient media is reduced by sterilization prior to sterilization and the sulphate is added at the beginning of the fermentation and / or for a further period to prevent further reduction. fermentation. Uzgorod Manufacturing and Printing Company, Proektnaja 4, Uzgorod »6060, Price“ 2,40 Kčs
CS84355A 1983-01-20 1984-01-17 Method of nourserotricine fermentation production CS258414B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DD83247370A DD233753A3 (en) 1983-01-20 1983-01-20 PROCESS FOR PRODUCING NOURSEOTHRICIN

Publications (1)

Publication Number Publication Date
CS258414B1 true CS258414B1 (en) 1988-08-16

Family

ID=5544502

Family Applications (1)

Application Number Title Priority Date Filing Date
CS84355A CS258414B1 (en) 1983-01-20 1984-01-17 Method of nourserotricine fermentation production

Country Status (5)

Country Link
JP (1) JPS6062987A (en)
BG (1) BG49225A1 (en)
CS (1) CS258414B1 (en)
DD (1) DD233753A3 (en)
SU (1) SU1451165A1 (en)

Also Published As

Publication number Publication date
SU1451165A1 (en) 1989-01-15
DD233753A3 (en) 1986-03-12
BG49225A1 (en) 1991-09-16
JPS6062987A (en) 1985-04-11

Similar Documents

Publication Publication Date Title
Castro-Alonso et al. Microbially induced calcium carbonate precipitation (MICP) and its potential in bioconcrete: microbiological and molecular concepts
Krajewska Ureases I. Functional, catalytic and kinetic properties: A review
Vekariya et al. Bacterial concrete: new era for construction industry
Dhami et al. Synergistic role of bacterial urease and carbonic anhydrase in carbonate mineralization
WO2006070944A3 (en) L-glutamic acid-producing microorganism and a method for producing l-glutamic acid
Oren Anaerobic degradation of organic compounds at high salt concentrations
CN103666426B (en) Oil field water biological treatment agent and preparation method thereof and using method
WO2022213898A8 (en) Caproic acid-producing bacterium capable of utilizing various substrates and use thereof
Osborne et al. Inhibition of malolactic fermentation by Saccharomyces during alcoholic fermentation under low‐and high‐nitrogen conditions: a study in synthetic media
EA029851B1 (en) Method for producing authigenic rock mineral for altering rock hydrology
CS258414B1 (en) Method of nourserotricine fermentation production
Auling Manganese: function and transport in fungi
Patidar et al. Effect of nutrients on biomass activity in degradation of sulfate laden organics
EP1233071A3 (en) Organic nitrogen-containing composition and fertilizer comprising the same
CN1406273A (en) Microorganism producing 5&#39;-inosinic acid and process for producing 5&#39;-inosinic acid using the same
EP1233070A3 (en) Method for producing L-glutamic acid
WO2009054368A1 (en) Method for washing soil containing cyanogen compound, and microorganism for use in the washing method
WO2021142491A3 (en) Therapeutic engineered microbial cell systems and methods for treating hyperuricemia and gout
ATE479766T1 (en) METHOD FOR OBTAINING A BASIC AMINO ACID FROM A FERMENTATION BROTH
Watteau et al. Iron solubilization by mycorrhizal fungi producing siderophores
Zaccone et al. Microbial hydrolysis of polysaccharides and organic phosphates in the Northern Adriatic Sea
CN111019859B (en) Application of L-arginine as chemotactic substance and mass swimming substance of bacillus cereus
Tomiyama et al. Characteristics of newly isolated nitrifying bacteria from rhizoplane of paddy rice
Carels et al. The effect of changes in pH on phosphate and potassium uptake by Monascus rubiginosus ATCC 16367 in submerged shaken culture
CN102719495A (en) Method for fermenting and preparing L-lactic acid by adding different neutralizers