CS258414B1 - Method of nourserotricine fermentation production - Google Patents
Method of nourserotricine fermentation production Download PDFInfo
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- CS258414B1 CS258414B1 CS84355A CS35584A CS258414B1 CS 258414 B1 CS258414 B1 CS 258414B1 CS 84355 A CS84355 A CS 84355A CS 35584 A CS35584 A CS 35584A CS 258414 B1 CS258414 B1 CS 258414B1
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- ηπη
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IAND
HaseaHHe Η3θ6ρβτβΗΗΗHaseaHHe Η3θ6ρβτβΗΗΗ
Cnoco6 nonyHeHHH HoypaeoTpHUHHa OCnaCTb ΠρΗΜβΗβΗΗΗ Η3θ6ρβΤβΗΗΗ Η3θ6ρετβΗΗβ OTHOCHTCH K nOJiyHeHHJO ΟΤρβΠΤΟΤρΗΙίΗΗΟΒΟΓΟ ΒΗΤΗβΗΟΤΗΚβ HOypaeOTPH“ΗΗΗβ πγτβΜ uejienanpaBJíeHHoro BO3fleňcTBHH Ha MHKpoGHonormecKyio φερΜβΗΤΗΗΗ».H3o6peTeHne ΗΜεετ 3havenue %ηη MHKpoCHOJiorHMecxoft npoMbmuieHHOCTH.Cnoco6 nonyHeHHH HoypaeoTpHUHHa OCnaCTb ΠρΗΜβΗβΗΗΗ Η3θ6ρβΤβΗΗΗ Η3θ6ρετβΗΗβ OTHOCHTCH K nOJiyHeHHJO ΟΤρβΠΤΟΤρΗΙίΗΗΟΒΟΓΟ ΒΗΤΗβΗΟΤΗΚβ HOypaeOTPH "ΗΗΗβ πγτβΜ uejienanpaBJíeHHoro BO3fleňcTBHH Ha MHKpoGHonormecKyio φερΜβΗΤΗΗΗ» .H3o6peTeHne ΗΜεετ 3havenue% ηη MHKpoCHOJiorHMecxoft npoMbmuieHHOCTH.
XapaKTepwcTHKa H3BecTHbix TexHnuecKHX peuieHHň B CBH3H C 6HOCHHTe3OM BTOpHHHblX MeTaGOJIHTOB B JIHTepaType yKaaWBaiOT Ha TpH .ocHOBHbix 4>aKTOpa penpeccHBHoro bjihhhhh , oxasbiBeeMoro 1. rnioK03oň h npyrHMH βηλπμη caxapa 2. aMHHOKHCJIOTaMH H aMMOHHňHblMH COJIHMH H · 3. φοεφβτοΜ (Martin, J.F. h'A.L. Demaín: Control of antibiotie biosynthesis. Microbiol,XapaKTepwcTHKa H3BecTHbix TexHnuecKHX peuieHHň CBH3H B C B 6HOCHHTe3OM BTOpHHHblX MeTaGOJIHTOB JIHTepaType yKaaWBaiOT Ha TPH .ocHOBHbix 4> aKTOpa penpeccHBHoro bjihhhhh, oxasbiBeeMoro first rnioK03oň h npyrHMH βηλπμη caxapa second aMHHOKHCJIOTaMH H · H aMMOHHňHblMH COJIHMH third φοεφβτοΜ (Martin JF h'AL Demain : Control of antibiotics biosynthesis.
Rev. 44, 230-251 (1980)). B cnyuae GHocHHTeaa MHKpoopraHH3MOM Streptomyces noursei IMET JA 3890 b ΟΤρβΠΤΟΤρΗΙίΗΗΟΒΟΓΟ ΒΗΤη6Η0ΤΗΚΒ HOyp3eOTpHBHHa H3BeCTHO, MTO aMHHOKHCJlOTbl HaMMOHHňHbie cojih οτρΗΐχβτβΠΒΗΟ bjihhmt na 3TOT GHOcHHTes (Gráfe U., Stendal. A.,Bocker, Η. ,h Thrum, H: Regulative intluence Of o-aminobenzoic acid (OABA)on the biosynthesis of noursethricin in cultures of Streptomyces nourseiJA 3890 b, Z. Allg. Mikrobiol. 20, 185-194 (1980) h iiHTHpyeMaH τβΜ xe JiHTe—paTypa). ‘Roar. 44, 230-251 (1980)]. B cnyuae GHocHHTeaa MHKpoopraHH3MOM Streptomyces noursei IMET JA 3890 b ΟΤρβΠΤΟΤρΗΙίΗΗΟΒΟΓΟ ΒΗΤη6Η0ΤΗΚΒ HOyp3eOTpHBHHa H3BeCTHO MTO aMHHOKHCJlOTbl HaMMOHHňHbie cojih οτρΗΐχβτβΠΒΗΟ bjihhmt on 3This GHOcHHTes (Chart U. Stendal. A., Bocker, Η., H THRU H Regulative intluence Of o-aminobenzoic acid (OABA) on the biosynthesis of noursethricin in cultures of Streptomyces nourseiJA 3890 b, Z. Allg., Microbiol 20, 185-194 (1980). '
Iloxa b xavecTBe ηΟτομηηκβ aaoTa b nHTaTenbHbix cpejiax HcnojibaoBanH ΗΗτρβτ aM- -ΜΟΗΗΗ (cm. Gráfe et ál. 1980). B stom coepjiHeHHH nojiOBHHa aaÓTa He npeflCTaB-JieHa B ΒΗηβ HOHOB aMMOHHH. ' "Iloxa b xavecTBe ηΟτομηηκβ aaoTa b nHTaTenbHbix cpejiax HcnojibaoBanH ΗΗτρβτ aM- -ΜΟΗΗΗ (cm. Gráfe et al. 1980). B stom coepjiHeHH nojiOBHHaaaTa He npeflCTaB-JieHa B ΒΗηβ HOHOB aMMOHHH. ''
HeflOCTaTKOM ΤβΧΗΗΗβΟΚΟΓΟ ΠρΗΜβΗβΗΗΗ HBJlHeTCH TO, HTO HHTpaT aMMOHHH ΗΒΠΗβΤ—ch BspbiBHaTbiM BemecTBOM h ncnojib3OBaHne ero flonycxaeTCH tojibko b coveTaHHHc TpyfloeMKHMH MepaMH 6e3onacHocTH. KpoMe τογο, y^ajiocb oSHapyxHBaTb BφΗΠΜ'ρβτε xyjibTypbi b Konye jiaSopaTopnoií φβρΜβΗτβΐίΗΗ He Conee 300 μκγ Hoyp- 258414 2 3eoTpHUHHa Ha μπ Tojibxo npH npHSaaneHHH oTHocHTejibHO floporHX a Bpefíoax. nnfi ,oxpyxcaiomeň cpeflbí ηηγη6ητοροβ AbixaHHH h nepenoca ημηηοχηοποτ, HanpHMep,o-aMHHo6eH3oňHoň KHcnoTbi (OABA) (Grafe, U., Bocker, H,, Reinhardt, G. κThrum, H.: Regulative Beeinflussung der Nourseothricinbiosynthese durcho-Amínobenzoesaure in Kultuten des Streptomyces nourséi JA 3890 b. Z. Allg.Mikrobíol. 14, 659-673 (1974)). H3BeCTHO, HTO H36bITOHHblň φοεφβΤ OTpHAaTeJIbHO ΒΠΗΗβΤ Ha : 6HOCHHTe3 pH/ja bto*PHMHbix MeTaSojiHTOB (Martin, J.F. : Control of antibíotíc bíosynthesis by *phosphate. Adv. Biochern. Eng, 6, 105-127 (1977); Weinberg.D.: Secondary me-tabolism: regulation by phosphate and trace elements, Folia Microbiol,. 23,496-504 (1978)). IIoaTOMy, HexoTopwe TexHHHecKue nponeccw Mnxpo6HOJiornnecKoftφβρΜβΗΤΗΐχΗΗ flJIH ΠΟΠΥΊβΗΗΗ βΗΤΗβκΟΤΗΚΟΒ npOBOflHT npH ΚΟΗΑβΗΤρ ΑΐχΗΗΧ φΟεφβΤβ,cyfíonTHManbHbix απη ροοτβ MHxpoGHonorHHecxoro npoAyneHTa. B TexHHHecxtix φερηMeHTaiwHx απη nojiyueHHH BTopHHHbix ΜβτββαπΜτοΒ, xax npaBuno, HcnontsyroT Ta—KHe KOMnoHeHTbi cpeflbí pacTHTenbHoro η χοϊβοτηογο nponcxoxAeHHH, xax xpaxMan,coeBbiň ηιροτ, xyxypysHbiii sxcTpaxT, Menacca, Mstctíoň axcTpaxT η AP· 06®ee co-AepwaHHe φοοφβτε h GHonorHHecxas AocTynHOcTb (pacTBOpHMocTb) KoMnnexcHorocy6cTpara pa3AHHHoe b 3aBHcnMocTH ot ero npoHcxoxfleHHH h oGpaGoTXH.HeflOCTaTKOM ΤβΧΗΗΗβΟΚΟΓΟβΗΗΗ HBJlHeTCH TO, HTO HHTpaT aMMOHHH ΗΒΠΗβΤ —ch BspbiBHaTbiM BemecTBOM h nbib3OBaHne ero flonycxaeTCH tojibko b coveTaHHHc TpyfloeMKHMH MepaMH 6e3onacHocTH. C apy apy apy apy apy apy apy apy apy apy apy apy apy C C C C C C C C C C C C oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj nnfi, oxpyxcaiomeň cpeflbí ηηγη6ητοροβ AbixaHHH h nepenoca ημηηοχηοποτ, HanpHMep, o-aMHHo6eH3oňHoň KHcnoTbi (GABA) (Graf, U., Bocker, H ,, Reinhardt, G. κThrum, H .: Regulative Beeinflussung der durch Nourseothricinbiosynthese-Amínobenzoesaure in Kultuten des Streptomyces nourséi JA 3890 b. Z. Allg. Microbiol., 14, 659-673 (1974). H3BeCTHO, HTO H36bITOHHbl φοεφβΤ OTpHAaTeJIbHO ΒΠΗΗβΤ Ha: 6HOCHHTe3 pH / ja bto * PHMHbix MeTaSojiHTOB (Martin, JF: Control of antibiotic biosynthesis by * phosphate. Adv. Biochern. Eng, 6, 105-127 (1977); Secondary metabolism: regulation by phosphate and trace elements, Folia Microbiol., 23,496-504 (1978). IIoaTOMy, HexoTopwe TexHHHecKue nponeccw Mnxpo6HOJiornnecKoftφβρΜβΗΤΗΐχΗΗ flJIH ΠΟΠΥΊβΗΗΗ βΗΤΗβκΟΤΗΚΟΒ npOBOflHT npH ΗΤΑβΗΤρ ΑΐχΗΗΧ φΟεφβΤβ, cyphionTHManbHbix απη ροοτβ MHxpoGHonorHHecxoro npoAyneHTa. B TexHHHecxtix φερηMeHTaiwHx απη nojiyueHHH BTopHHHbix ΜβτββαπΜτοΒ, xax npaBuno, HcnontsyroT Khe Ta-KOMnoHeHTbi cpeflbí pacTHTenbHoro η χοϊβοτηογο nponcxoxAeHHH, xax xpaxMan, coeBbiň ηιροτ, xyxypysHbiii sxcTpaxT, Menacca, Mstctíoň axcTpaxT η · AP 06®ee co-AepwaHHe φοοφβτε h GHonorHHecxas AocTynHOcTb (pacTBOpHMocTb ) CoMnnexcHorocy6cTpara pa3AHHHoe b3aBcnMocTH otro npoHcxoxfleHHH h oGpaGoTXH.
TaxHM o6pa3OM, oGmuň φσοφετ pa3jiHMHoro coÁepxtaHHH, nepeHocHMbiň nepes xom-nJieKCHbíe cyGcTpaTbi b cpeny rnyfíHHHoro xyjibTHBHpoBaHHH, npHcyTcTByeT b nana-ne φβρΜβΗΤΘΗΗΗ B BHfle paCTBOpeHHOrO, ΗβΜβΑΠβΗΗΟ flOeTynHoro φοεφβτβ. ΠρΗΒβΑβΗΗΜβ oGcTOHTenbCTBa npHi .(.HnuanbHo aaTpyflHHioT cTaHflapTH3aun» nHT.aTenb-bbix cpeff. EHOJioruvecKaa flocTynHOCTb φοοφβτε ΗΜββτ pemaiomee SHaneime ffiut bm-xofla wenaeMbix BTOpHHHbix ΜβτβδοηΗτοΒ, Tax xax, c ogHoft cTOpOHbí, onpegeneHHoeKojiHvecTBO φοεφβτβ HeoGxoAHMo απη počija MHxpoopraHH3MOB—npoAyneHTOB, a, cAPyroň cTopoHbí, cjihiukom Bbicoxne HauajibHbie KOHAeHTpauHH pacTBopHMoro φοοφβτβMoryT yrHeTaTb oGpasoBaHHe BTopnvHbix MeTaGonuTOB., Πβτθητ DD-WP 155 239npeTeHAyer Ha npHMeneHHe xoHneHTpanHH coAepxamHxcn b xyjibTypaJibHoft xhakocthφοοφετΗΗχ hOhob xax perynHpyeMy» BenHHHHy ρ,ηη cTaGunnsamui MHXpoGHonorHuec-xoň xyrtbTypbi HenpepbiBHoro AeňcTBHH. 3tot cnocoG, oflHaxo, othochtch τοπβκοκ φερΜβΗτβτΗΒΗΟΐ^ nojiyneHHio GHOMaccbi, npnHeM aanaHHoe 3HaHeHHe KOHiteHTpauHHφοεφβτβ cneAyeT ycTáHaanHBaTb b npenenax 20-60 μγ/π φοοφβτβ. ΠρΗΜβΗβΗΗβ sto—to H3o6peTeHHH κ peryjiHpOBaHHio GHOCHHTeaa βτορηηηηχ ΜβτβθοπκτοΒ, b waČTHOc-TH B ΜΗΧροθΗΟΠΟΓΗΗβΟΧΗΧ XyHbTypaX C nepHOAHWeCXHM ΠΗΤΗΗΗβΜ, HeH3BeCTHO«3φφεχτω perynHposaHHH φοεφβτβ Ha nponecc 6HOcnHTe3a Hoyp3eoTpnAHHa HeHSBecT—HbI,TaxHM o6pa3OM, oGmuň φσοφετ pa3jiHMHoro coÁepxtaHHH, nepeHocHMbiň nepes Xom-nJieKCHbíe cyGcTpaTbi b cPen rnyfíHHHoro xyjibTHBHpoBaHHH, nana-b npHcyTcTByeT not φβρΜβΗΤΘΗΗΗ B BHfle paCTBOpeHHOrO, ΗβΜβΑΠβΗΗΟ flOeTynHoro φοεφβτβ. ΠρΗΒβΑβΗΗΜβ oGcTOHTenbCTBa npHi. (. HnuanbHo aaTpyflHHioT cTaHflapTH3aun »nHT.aTenb-bbix cpeff. EHOJioruvecKaa flocTynHOCTb φοοφβτε ΗΜββτ pemaiomee SHaneime ffiut BM-xofla wenaeMbix BTOpHHHbix ΜβτβδοηΗτοΒ, Tax XAX c ogHoft cTOpOHbí, onpegeneHHoeKojiHvecTBO φοεφβτβ HeoGxoAHMo απη počija MHxpoopraHH3MOB-npoAyneHTOB and, cAPyroň cTopoHbí, cjihiukom Bbicoxne HauajibHbie KOHAeHTpauHH pacTBopHMoro φοοφβτβMoryT yrHeTaTb oGpasoBaHHe BTopnvHbix MeTaGonuTOB., Πβτθητ DD-WP 155 239npeTeHAyer Ha npHMeneHHe xoHneHTpanHH coAepxamHxcn b xyjibTypaJibHoft xhakocthφοοφετΗΗχ hohoba XAX perynHpyeMy »BenHHHHy ρ, ηη cTaGunnsamui MHXpoGHonorHuec-XON xyrtbTypbi HenpepbiBHoro AeňcTBHH. 3This cnocoG, oflHaxo, othochtch τοπβκοκ φερΜβ ,τβτΗΒΗΟΐ ^ nojiyneHHio GHOMaccbi, npnHeM aanaHHoe 3HaHeHHe KOHiteHTpauHHφοεφβτβ cneAyeT ycTáHaanHBaTb b npenenax 20-60 μγ / π φοοφβτβ HHH κ peryjiHpOBaHHio GHOCHHTeaa βτορηηηηχ ΜβτβθοπκτοΒ b waČTHOc TH-B ΜΗΧροθΗΟΠΟΓΗΗβΟΧΗΧ XyHbTypaX C nepHOAHWeCXHM ΠΗΤΗΗΗβΜ, HeH3BeCTHO «3φφεχτω perynHposaHHH φοεφβτβ Ha nponecc 6HOcnHTe3a Hoyp3eoTpnAHHa HeHSBecT-HBI,
Renb H3o6peTeHHHRenb H3o6peTeHHH
IfejibK) Η3θ6ρετβΗΗΗ HBJíHeTca ycTpaHeHHe HenocTaTkoB paHee onHcaHHbix cnocoGoBnonyneHHH Hoyp3eoTpHAHHa h yBejiHveHHe xOHneHTpanHň HoypaeoTpHAHHa b τθχηη-necKHX φβρΜβΗΤθίξΗΗχ nyreM BO3AeňcTBHH Ha npoyecebi φερΜβΗτβιΐΗΗ. |H3JI0»eHHe CyiUHOCTH Η3θ6ρθΤβΗΗΗ B ocHOBy H3o6peTeHHH nonoweHa eanana npeACTaBHTb TexHHnecxH BbiroflHbift cnoco6AJiH npoMbimneHHoro npoH3BOAČTBa HoypaeoTpHAHHa. HeoxHAaHHO 6ωπο HaňAeHO, htoπρπ φβρΜβΗΤβΗΗΗΧ flJlH nOnyHeHHH HOypaeOTpHAHHa nocne ΠΟΗΗΧβΗΗΗ HandjIbHOňxoHneHTpaAHH φοοφβτβ HHXce cHHTaBnierocH paHee onTHManbHbíM SHaueHHH h nocne-Ay»mero npHGaBneHHH cooTBeTCTBywuiero ηπη Gonee Bbicoxoro xonHHecTBa φοοφβτβb npoAecce xynbTHBHpOBaHHH, A°6aBJiHeMoro no onpeAeneHHOMy pexcuMy ΒρβΜβΗΗ,nonynaioTCH 3HanHTenbHO Gonee BbicoKHe Bbixona Hoýp3eoTpnnnHa, 258414 3 ΚροΜβ τογο 6bino HaňflGHO, πτο b CaCOj— coflepwauteň nHTaTenbHoft cpene npH sa-M6H6 HCTOMHHKa a3OTa HHTpaTa aMMOHHH Ha cynbiJiaTcoAepxaiiiHft cynb4>aT ημμοηηηb cooTHomeHHH, CTexHOMeTpHMecKOM b othoqichhh coflepxaHHH aaoTa, HananbuaHKOHiíeHTpauHH φοεφβτβ cHHwaeTCH ót 15 μγ/π φοεφβτΗΟΓΟ P flo 1-3 μγ/π b coO-CTBeHHO H6H9M6HHOÍÍ cpefle nocjie CTeptUIHSaitHH BOffHHbiM napOM ΠΟΑ ΑβΒΠβΗΗβΜ. B oSmeM HaMajibHyw KOHueHTpauHio pacTBopHMoro, Οηοπογηηθοκη ΗβΜβΑΠβΗΗΟ flocTyn-Horo φοεφβτβ b npocTepwiH3OBaHHoft cpe#e moxho CHHSHTb cooTBeTCTBywmHM cor-nacoBaHneM pexHMa creptuíMsamiH h coAepxaHHH cyπbφaτHbκ hohob h HeopraHHHec-khx coneň ηπη hohob ΜβτβπποΒ, flaianHX bmgctg c φοεφβΤΗΜΜΗ ηπη cyj^aTHbíMHHOH3MH TpyflHO paCTBOpHMbie OCaflKH. ΠρΗΜΗΗΟή chhxghhh Taxoň HaMajibHoň KOHiteHTpaiíHH b cnynae npHcyTCTBHH AOCTa-ΤΟΗΗΟΓΟ KOJIHHeCTBa εγΠΒφβΤΗΕΙΧ HOHOB OKaaanCH CflBHr KHCJ1OTHOCTH CJia6OKHCnoňnHTaTejibHoň cpe/ju b CTopoHy menoMHhix 3HaneHHň pH b npouecce cTepHnH3auHH,Bbi3biBaeMbiň ocaxneHHeM CaS04 u οβη33ηημμ c sthm yuaneHHeM hs cpaam φΗ3Ηθπο-rmeCKH KHCJIbK ΟνΠΒφβΤΗΜΧ HOHOB. COOTBGTCTBeHHO HaHajlbHan KOHmeHTpaUHHpacTBopHMoro ΗΠΗ 6ΗΟΠΟΓΗΗβCKH flOCTyiIHOrO φοοφβτβ CHHMaeTCH TaKXe BCJieACTBHeycTaHOBKH tuenouHoro sHaueHHH pH nepeA cTepnnn3auHeň. yBenmeHHe 3HaqeHHH pHoeecneHHBaeT coagaHHe CnaronpHHTHbix ycnoBHň ρ,ηκ ocaxAeHHH φοοφβτβ c onpene-neHHbíMH npHcyTCTBynatHMH b nHTaTenbHoft cpefle ηοηθμη ΜβτβπποΒ. OcaxAeHH» φοο-φβτ coAepxanufX ocaAKOB cnocoCcTByeT HanpaeneHHOe πρΗθββπβΗΗβ tbkhx hohob Me-TajuioB, khk, HanpHMep, xeneao (III), antoMHHHň (III), MarHHft (II), KanbUHft(II), ahhk (II) h APyrne.IfejibK) Η3θ6ρετβΗΗΗ HBJíHeTca ycTpaHeHHe HenocTaTkoB Pahea onHcaHHbix cnocoGoBnonyneHHH Hoyp3eoTpHAHHa h yBejiHveHHe xOHneHTpanHň HoypaeoTpHAHHa b τθχηη-necKHX φβρΜβΗΤθίξΗΗχ Nyree BO3AeňcTBHH Ha npoyecebi φερΜβΗτβιΐΗΗ. | H3JI0 »eHHe CyiUHOCTH Η3θ6ρθΤβΗΗΗ B ocHOBy H3o6peTeHHH nonoweHa eanana npeACTaBHTb TexHHnecxH BbiroflHbift cnoco6AJiH npoMbimneHHoro npoH3BOACTBa HoypaeoTpHAHHa. HeoxHAaHHO 6ωπο HaňAeHO, htoπρπ φβρΜβΗΤβΗΗΗΧ flJlH nOnyHeHHH HOypaeOTpHAHHa nocne ΠΟΗΗΧβΗΗΗ HandjIbHOňxoHneHTpaAHH φοοφβτβ HHXce cHHTaBnierocH Pahea onTHManbHbíM SHaueHHH h nocne-Ay »mero npHGaBneHHH cooTBeTCTBywuiero ηπη Gone Bbicoxoro xonHHecTBa φοοφβτβb npoAecce xynbTHBHpOBaHHH, A ° 6aBJiHeMoro well onpeAeneHHOMy pexcuMy ΒρβΜβΗΗ, nonynaioTCH 3HanHTenbHO Gone BbicoKHe Bbixona Hoýp3eoTpnnnHa, 258414 3 ΚροΜβ τογο 6bino HaňflGHO, πτο b CaCOj- coflepwauteň nHTaTenbHoft cPen Nph-M6H6 HCTOMHHKa a3OTa HHTP aMMOHHH Ha cynbiJiaTcoAepxaiiiHft cynb4> aT ημμοηηηb cooTHomeHHH, CTexHOMeTpHMecKOM b othoqichhh coflepxaHHH AAOT, HananbuaHKOHiíeHTpauHH φοεφβτβ cHHwaeTCH formylimidazolyl 15 μγ / π φοεφβτΗΟΓΟ P flo 1-3 μγ / π b coO-CTBeHHO H6H9M6HHOÍÍ cpefle nocjie CTeptUIHSaitHH BOffHHbiM napOM ΠΟΑ ΑβΒΠβΗΗβΜ. B eighth HaMajibHyw KOHueHTpauHio pacTBopHMoro, Οηοπογηηθοκη ΗβΜβΑΠβΗΗΟ flocTyn-Horo φοεφβτβ b npocTepwiH3OBaHHoft CPE # e Moxhe CHHSHTb cooTBeTCTBywmHM cor-nacoBaHneM pexHMa creptuíMsamiH h coAepxaHHH cyπbφaτHbκ hohoba h HeopraHHHec-khx Conen ηπη hohoba ΜβτβπποΒ, flaianHX bmgctg c φοεφβΤΗΜΜΗ ηπη cyj ^ aTHbíMHHOH3MH TpyflHO paCTBOpHMbie OCaflKH. ΠρΗΜΗΗΟή chhxghhh taxon HaMajibHoň KOHiteHTpaiíHH b cnynae npHcyTCTBHH AOCTa-ΤΟΗΗΟΓΟ KOJIHHeCTBa εγΠΒφβΤΗΕΙΧ hohoba OKaaanCH CflBHr KHCJ1OTHOCTH CJia6OKHCnoňnHTaTejibHoň CPE / ju b CTopoHy menoMHhix 3HaneHHň pH b npouecce cTepHnH3auHH, Bbi3biBaeMbiň ocaxneHHeM CaS04 at οβη33ηημμ c sthm yuaneHHeM cpaam φΗ3Ηθπο HS-rmeCKH KHCJIbK ΟνΠΒφβΤΗΜΧ hohoba. COOTBGTCTBeHHO HaHajlbHan KOHmeHTpaUHHpacTBopHMoro ΗΠΗ 6ΗΟΠΟΓΗΗβCKH flOCTyiHiOrH K ο β τ H H H H s s s s s s s s enou s ue ue ue ue ue ue ue.. yBenmeHHe 3HaqeHHH pHoeecneHHBaeT coagaHHe CnaronpHHTHbix ycnoBHn ρ, ηκ ocaxAeHHH φοοφβτβ c onpene-neHHbMH npHcyTCTBynatHMH b nHTaTenbHoft cpefle ηοηθμη ΜβτβπποΒ. Ax ο oc A A A A A A A x x x x x x x x x x x x x pa pa pa pa pa pa pa pa pa pa----------
OcoCeHHO 6nar0npHHTHoe BAHHHHe Ha ocaxAeHne φοεφβτβ oxasbtBaeT npHMeHHeMbiňB OOAblDHHCTBe TGXHHMCCKHX φβρΜβΗΤΗΑΗή ΜβΤΟΑ ΟΤβρΗΠΗ9βΑΗΗ ΗβΠΟΟρβΑΟΤΒβΗΗΟBO3AeňcTBywiuHM BOflHHbíM napoM πόα flaBJíeHHeM, a Tarate npHcyTCTBHe CaCOj, xo-Topbiň HcnoAbsyeTGH b xauecTBe 6y4>epa απη perynHpoeaHHH pH b npouecce φβρΜβΗ*ΤβΑΗΗ.OcoCeHHO 6nar0npHHTHoe BAHHHHe Ha ocaxAeHne φοεφβτβ oxasbtBaeT npHMeHHeMbiňB OOAblDHHCTBe TGXHHMCCKHX φβρΜβΗΤΗΑΗή ΜβΤΟΑ ΟΤβρΗΠΗ9βΑΗΗ ΗβΠΟΟρβΑΟΤΒβΗΗΟBO3AeňcTBywiuHM BOflHHbíM πόα flaBJíeHHeM drinks, and tara npHcyTCTBHe CaCOj, XO-Topbiň HcnoAbsyeTGH b xauecTBe 6y4> EPA απη perynHpoeaHHH pH npouecce φβρΜβΗ b * ΤβΑΗΗ.
CoAepxaHHe φοοφβτβ b o6meM moxho CHHSHTb τβκχβ yMeHbnieHHeM aojih φοοφβτοο-Aepxanpix κομππθκοημχ cyOcTpaTOB. Tax, HanpHMep, HcnonbdyeMbift b φβρΜβΗτβΗΗΗHoypseorpHAHHa sKCTpaier coesoro rnpora couepXHT οκοπο 8 r φοεφβΤΗΟΓΟ φοοφο-pa rfa 1 r uipoTa. B nepeuHcneHHbix Bbme ycnoBHHx HananbHaH xoHueHTpauHH pacTBopeHHoro ηπη po-cTynHoro φοεφβτβ 6yueT HacTonbKo HHsxoft, hto bo bchkom cnyuaé επβΑΗφΗΜβε-ΚΗβ Α·ΠΗ HOypaeOTpHAHHOnpOAyiWPyWWHX MHKpOOpraHHSMOB BepXHHe ΠρβΑβΠΒΗΟ-ΑΟ-nycTHMbie sHaneHHH φοοφβτβ ocTaroTCH HHxe βτογο npeuena. TaxHM oOpaSoM, Ta-ΚΗβ He6oJIbniHe KOHUeHTpaUMH φοοφβτβ ΠΟ3ΒΟΠΗΙ0Τ nyTeM ΠρΗββΒΠΘΗΗΗ ΟΠρβΑβΠβΗΗΟ-ro KonHMecTBa φοοφβτβ nocne cTepHnHsauHH nonyuHTb cpeuy, b sHanHTenbHoň οτβ-neHH cTaHAapTHaoBaHHyio no φοεφβτγ. ΚροΜβ τογο, pexHM πonauη φοεφβτβ ποββοπη-βΤ ynpaBJIHTb φΟβφβΤΗΜΜ ΟβΜβΗΟΜ MHKpOOpraHHSMOB ΤΒΚΗΜ OCpaSOM, ΗΤΟβΜ MOJKHO6bl.no nonyHHTb npOAyKT C BblCOKHM BbIXOAOM, ΦοεφβΤ ΜΟΧβΤ nOAdBaTbCH B ΒΗΑβpacTBopa ηπη TBepAoro BemecTaa ηπη cycneH3HH. Φοοφβτ ΜΟχβτ npHcyrcTBOBaTtΠΗ6ο B φορΜβ CBO6OAHbIX φσοφβΤΗΒΙΧ HOHOB, ΠΗ0Ο B ΒΗΑβ ΧΗΜΗΗβΟΚΗ ΗΠΗ φΗ3ΗΗβΟΚΗcBnsaHHoro φοοφβτβ. Ilonana φοοφθτβ ηπη φοεφΒΤΟΟΑβρχβηΐΗχ BemecTB ocymecTBna-eTCH Β 33BHCHMOCTH ΟΤ peXHMa ΠΟΑβΗΗ nocne εΤβρΗΠΗ3βΑΗΗ ΗΠΗ ΒΟ ΒρβΜΗ φερΜβΗ-TaunH cnocoÓOM pasoBoro η/ηπη MHoropasoBoro η/ηπη HenpepuBHoro πρΗββΒπβΗΗπ.HenpepbIBHOe ΙίρΗθβΒΠβΗΗβ φοοφβτβ ΜΟΧβΤ OCymGCTBJIHTbCH C paanHHHOft CKOpOCTbIO.ΠΟΑβΗΗ ΠΟ ΒρβΜΗ φβρΜβΗΤβΑΗΗ. r, Β KanecTBe ποΟοπηογο ycnoBHH cneAyeT' oÓecnenHBaTb, hto6m cy6cTpaTU ηπη yc-ποβηη oKpyxaxníeň cpeflbí npH noAane φοοφβτβ, pe3ynbTHpyR»tHň H3 sToro poct ηπηnoBbnueHHan aKTHBHocTb oĎMeHa BemecTB He BbisbiBanu HeČnaronpHHTHoft πημητβαηηΑπη o6pa3OBaHHH npoAyKTa. TaKHM o6pa3OM yAaeTcn β ιΠΗρόκοή ετβπβΗΗ Hcnepnu- 258414 4 saTb enonorHHecKyio BO3Mo»HocTb HoypaeoTpHqHHooĎpaaoBaTejiH «jih obpaaoBaHHHnpoflyKTa h yBenHHHBaTb Bbixon nponyKTa. npHMepw ocymecTBJíeHHH . Η3θ6ρετβΗΗβ óonee ηο,αροδΗο noHCHHeTCfl Ha οοηοβθηηη npHMepos ocyiaecTBneHun. 1) ΙΐΗθφΗΠΗ3ΗροΒ3ΗΗΗε Ha ποΊΒβ KOHcepBHposaHHbie cnopw, ceneKTaHTa NG 13-14Hoyp3eoTpHu;nHoo6pa3yiomeFo wTaMMa Streptomyces noursei IMET JA 3890b BMcesa-mt Ha noflxoflHiuyro arapoeyio cpeňy, HanpHMep, arap OMepcona. ΠρΗΜθρκο nepeai7-flHeBHbiň nepHOfl HHKyóHpoBaHHH npH 30°C BbipeaaioT ynacTKH MHnenHanbHoro ra-3OHa paaMepoM οκοπο 2 cm2, a hmh aaceBawT KynbTypy npeflBapHTenbHoro BwpaasH-BaHHB. Čpěna npensapHTenbHoro BWpamHBaHHH conepXHT Ha oflHH πκτρ Boflonpoeofl“Hoň Bona cnenyromHe BemecTBa: 40 r rnMKO3bi, 15 r o6e3MacneHHoft ccesoň MyKH,0,3 r KH2PO4, 5 r NaCl h 3 r CaCO3. KncnoTHOcTb cpenbi ycTaHasnuBaioT nepencTepunHaanHeň Ha 3HaneHne pH 6,5. 3Ty cpeny 3anHBaioT b 3aKpwBaeMwet saTHoftπροδκοή niHpoKoropnbie 6yTbuiKH eMKocTbio 500 μπ Kaxnaa b, KonHnecTBax no 50 μπ.CTepnjnH3auHio ocymecTBnHMT 35-MHHyTHbiM HarpeBOM no 121°C b aBTOKnaBe.CoAepxaHHe φοοφβτβ b o6meM moxho CHHSHTb τβκχβ yMeHbnieHHeM aojih φοοφβτοο-Aepxanpix κομππθκοημχ cyOcTpaTOB. Tax, HanpHMep, HcnonbdyeMbift b φβρΜβΗτβΗΗΗHoypseorpHAHHa sKCTpaier coesoro rnpora couepXHT οκοπο 8 r φοεφβΤΗΟΓΟφοοφο-pa rfa 1 r uipoTa. B nepeuHcneHHbix Bbme ycnoBHHx HananbHaH xoHueHTpauHH pacTBopeHHoro ηπη after-cTynHoro φοεφβτβ 6yueT HacTonbKo HHsxoft, HTO bo bchkom cnyuaé επβΑΗφΗΜβε-ΚΗβ Α · ΠΗ HOypaeOTpHAHHOnpOAyiWPyWWHX MHKpOOpraHHSMOB BepXHHe ΠρβΑβΠΒΗΟ-ΑΟ-nycTHMbie sHaneHHH φοοφβτβ ocTaroTCH HHxe βτογο npeuena. TaxHM oOpaSoM, Ta-ΚΗβ He6oJIbniHe KOHUeHTpaUMH φοοφβτβ ΠΟ3ΒΟΠΗΙ0Τ ΠρΗββΒΠΘΗΗΗ ΟΠρβΑβΠβΗΗΟ rivet-Ro KonHMecTBa φοοφβτβ nocne cTepHnHsauHH nonyuHTb cpeuy b sHanHTenbHoň οτβ-neHH cTaHAapTHaoBaHHyio φοεφβτγ no. ΚροΜβ τογο, pexHM πonauη φοεφβτβ ποββοπη-βΤ ynpaBJIHTb φΟβφβΤΗΜΜ ΟβΜβΗΟΜ MHKpOOpraHHSMOB ΤΒΚΗΜ OCpaSOM, ΗΤΟβΜ MOJKHO6bl.no nonyHHTb npOAyKT C BblCOKHM BbIXOAOM, ΦοεφβΤ ΜΟΧβΤ nOAdBaTbCH B ΒΗΑβpacTBopa ηπη TBepAoro BemecTaa ηπη cycneH3HH. BOοοφβτ ΜΟχβτ npHcyrcTBOBaTtΠΗ6ο B φορΜβ CBO6OAHbIX φσοφβΤΗΒΙΧ HOHOB, ΠΗ0Ο B ΒΗΑβ ΧΗΜΗΗβΟΚΗ ΗΠΗ φΗ3ΗΗβΟΚΗcBnsaHHoro φοοφβτβ. Ilonana φοοφθτβ ηπη φοεφΒΤΟΟΑβρχβηΐΗχ BemecTB ocymecTBna etch Β 33BHCHMOCTH ΟΤ peXHMa ΠΟΑβΗΗ nocne εΤβρΗΠΗ3βΑΗΗ ΗΠΗ ΒΟ ΒρβΜΗ φερΜβΗ-TaunH cnocoÓOM pasoBoro η / η ηπη MHoropasoBoro / ηπη HenpepuBHoro πρΗββΒπβΗΗπ.HenpepbIBHOe ΙίρΗθβΒΠβΗΗβ φοοφβτβ ΜΟΧβΤ OCymGCTBJIHTbCH C paanHHHOft CKOpOCTbIO.ΠΟΑβΗΗ ΠΟ ΒρβΜΗ φβρΜβΗΤβΑΗΗ. r Β KanecTBe ποΟοπηογο ycnoBHH cneAyeT 'oÓecnenHBaTb, hto6m cy6cTpaTU ηπη YC-ποβηη oKpyxaxníeň cpeflbí NPH noAane φοοφβτβ, pe3ynbTHpyR »THN H3 Stora honors ηπηnoBbnueHHan aKTHBHocTb oĎMeHa BemecTB He BbisbiBanu HeČnaronpHHTHoft πημητβαηηΑπη o6pa3OBaHHH npoAyKTa. TaKHM o6pa3OM yAaeTcn β ιΠΗρόκοή ετβπβΗΗ Hcnepnu- 258414 4 saTb enonorHHecKyio BO3Mo »HocTb HoypaeoTpHqHHooDpaaBaTejiH« southBaHHnpoflyKTa h yBenHHHBaTb Bbixon nponyKTa. npHMepw ocymecTBJieHHH. Θ3θ6ρετβΗΗβ óonee ηο, αροδΗο noHCHHeTCfl Ha οοηοβθηηη npHMepos ocyiaecTBneHun. 1) πθφΙΐΗΠΗ3ΗροΒ3ΗΗΗε Ha ποΊΒβ KOHcepBHposaHHbie cnopw, cenyKTaHTa NG 13-14Hoyp3eoTpHu; nHoo6pa3yiomeFo wTaMMa Streptomyces noursei Nep y ρ-H H H H bi H H H H H H H H H H cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm cm p p p p p p p p Boflonpoeofl "Ho Bona cnenyromHe BemecTBa: 40 r rnMKO3bi, 15 r o6e3MacneHHoft cecho MyKH, 0.3 r KH2PO4, 5 r NaCl h 3 r CaCO3. The pH of the cell is not pH 6.5. 3Ty cpeny 3anHBaioT b 3aKpwBaeMwet saToToBoioMyToBoMoMoMpoMoToMoMoMoToMoMoMoMoMoMoMoMoMoMoMoMoMoMoMoMo.
SacesHHwe KynbTypw npesapHTenbHoro BWpamHBaHHH MHKyGHpyiOT b Teueiwe 48 *ta-cob npn 29° C Ha KananKe. lípu πομοπ$η 150 μπ nonyneHHoro τηκημ 06pa3OM noceB-ηογο MaTepHana 3aceeawT 2500 μπ ochobhoé KynbTypbi b naGopaTopHoM φερΜβΗΤβ-'ropě. flna ochobhoé KynbTypbi Hcnonb3yioT cpeny, conepwamyio b οληομ πκτρε cneňywmneHHŤaTenbHbie BemecTBa: 32 r κβρτοφεπΒΗοτο KpaxMana, 29 r γπιοκοβμ, 11 r oBea-MacneHHOň coeBOÉ MyKH, lir (NH^íSO*. 2 r MgS0<7H20, 1 r NaCl, 6 r CaCO3h 0,5 r ZnSO4· 7Hž0. Ha 2500 μπ nHTaTenbHoň cpenbi πρη6ββπημτ 1 μπ βΗΤβφροΗβb xanecTBe neHoracHTenn Ha chhhkohoboé ocnoBe. KncnoTHocTb nepen erepHnHaa-HHeň ημθθτ 3HaneHHe pH 6,0. Ilocne cTepnnHaanHH sánonHeHHoro 250 μπ KynbTy-panbHoft WHflKocTH φβρΜθΗΤΗΤορβ npH 121°C b aBTOKnaBe h nocnenywmero oxnaatfle-hhh no 30°C cTapTÓByw KHcnoTHOCTb ycTaHaBnHBaioT, ot pH 8,4 Ha pH 6,8, npH-SaBnaa acenTHnecKyM noSaBKy 1 0%-hoé CTepHnbHOÉ cepHoň κηοποτμ. 3aceHHHbieφβρΜβΗτβηκοΗΗΜβ cpenm HHKyčHpyioT b TeneHHe 168 nacoB npn TeMnepaType 3O0C hckopocth aapanHH 2500 μπ BO3nyxa b MHHyry πρκ nepeMenruBaHHH c ηηοϊομ oCopo-tob 800 o6/mHh, CnycTH 1,5 uaca nocne aacesa ochobhoé KynbTypw πρκ noMonmnepHCTanbTHnecKoro Hacoca~no3aTopa HenpepwBKO πρη6ββπημτ pácTBop 4 h KH2P04Ha ΠΗΤρ ηΗΟΤΗΠΠΗρΟΒaHHOň BOnbI Β ΤβΠβΗΗβ 5 HaCOB CO CKOpOCTbM ΠΟΠΗΗΗ1,8 Μπ/nac; ecnen 3a bthm pacTBop πρΗδβΒππΜτ b TeneHHe 21 naca co CKopoc-TbM HO3HpOBaHHH 3,5 Mn/naC. Bo ΒρβΜΗ φβρΜβΗΤβΗΗΗ Β acenTHHeCKHX ycnOBHHX tie-pHonHnecKH 6epyT npo6w KynbTypanbHOň jkhhkocth η b hhx ΜβτοηοΜ n*«l^y3HH barap c Hcnonb3OBaHHeM MHKpoopraHH3Ma Bacillus ' subtilis ATCC 6633 b KanecT-Be TecT-MHKpoopraHH3Ma οπρεηεππιοτ conepxaHHe Hóyp3eoTpHnHH—ochob3hhh, rnwKO-3 w, aMMOHHííHoro a3OTa h TBepnwx Bewec.TB. napaMeTpw ρθ£, pH h conepxaHHH C0£β οτχοηππιΗΧ H3 annapaTa ra3OB KOHTponHpywT β χοηθ nponecca ΜετοηοΜ H3Mepe-hhh c perHCTpanHeň, CHHweHHe 3HaneHHH κηοποτηοοτη KynbTypanbHoÉ khhkocthHH»e SHaueHHH pH 5,8 npenoTBpamaioT npHbaBneHHeM cTepHnhHoro 5%-ηογο pacTBO-pa enKoro Harpa. fapH CHHDKeHHH KOHneHTpanHH ΓΠΜΚΟ3Μ fíO 5 r/π ΗΠΗ aMMOHHÉHOrO a3OTa ΠΟ 0,5 r/π,hto OTpaacaeTcn Ha yBenHneHHe H3MepeHHbix 3HaneHHÉ pH η ρθ·ι» a Tarase Ha chh-xeHHH conepacaHHH C02 β οτχοπηιηηχ ra3ax, πρΗδβΒππιοτ nepnonunecKH 25 r γπιοκο-3W ΗΠΗ .10 Γ (NH4)2SO4 B BHfle KOHneHTpHpOBaHHblX CTepHHbHblX paeTBOpOB.flanbHeňniyio φερΜβΗτβηΗΜ προΒοηπτ 6ea npH6aBneHHn ΚΗ^ΡΟ^ , γπιοκο3Μ η (ψΙ^)^δθ4 258414 5 b ycnoBHHX, Me OTjiHWHbix ot oCbíMHbix. Ilocne npeKpameHHH φβρΜβΗτβΑΗΗ B KynhTy-panbHoň xhakocth npucyTCTByioT eme ηβτκο o6HapyxHBaeMbie KonnuecTBa rn»KO3bi haMMOHHH. KOHneHTpannu HOyp3eOTpHUHH-OCHOBaHHH, OnpeflejjeHHbie B (JjHJIbTpaTaXxynbTyp oCohx pasjíHMHbix <J>epMeHTaitfiOHHbix nponeccoB, πρΗΒβΑβΗΜ b TaOnHue 1 . 2) JlHO$HJiH9HpoBai«n>iň Ha iTHOKoso-xejiaraHe KOHcepBHpoBaHHbiň μμαβαηΚ (eec 6pyT-to KOHcepBHpoBaHHoro coflepwHMoro - οκοπο 300 μγ) HaaBaHHoro b npHMepe 1Hoyp3eoTpHUHHOo6pa3ywmero urraMMa ετρβπτοΜΗηβτοΒ cycneHAHpyioT c 3 μπ 0,9Z-Horo pacTBopa xnopHCTóro HaTpHfl, 0,5 μπ 3Toň cycneHSHH cnyxHT b xanectBeHHOKynioMa απη 400 μπ cpeflbí npeflBapHTenbHoro KynbTHBHpoBaHHH nepBoň CTaAHH.Cpe#a npeflBapHTenbHoň xynbTypbi ΗΜββτ τοτ xe caMbiň cocTae η KHcnoTHocTb, htob npHMepe 1, OTy cpefly 3anHBaior b 3aKptiBaeMbie eaTHOň προδκοΗ uiHpoKoropnbieCyTbinxH eMKoeTb» 2500 μπ b KonHnecTBax no 400 μπ. CTepwiHsaAHio npoBOflHT35-MHHyTHbiM HarpeBOM ao 121°C b βΒτοκπβΒβ.SacesHHwe KynbTypw npesapHTenbHoro BWpamHBaHHH MHKyGHpyiOT b Teueiwe 48 * tai-cob npn 29 ° C Ha KananKe. lime tree ο 150 μπ nonyneHHoro τηκημ 06pa3OM noceB-ηογο MaTepHana 3aceeawT 2500 μπ KobbTypbi b onGopaTopHoM φερΜβΗΤβ -rope. fln ochobhoé KynbTypbi Hcnonb3yioT cpeny, conepwamyio b οληομ πκτρε cneňywmneHHŤaTenbHbie BemecTBa: 32 r κβρτοφοτο KpaxMana, 29 r γπιοκοβμ, 11 r BBea-MacneHo CoeBOÉ MyKH, lir (NH4 SOSO *. 2 r MgS0 <7H20, 1 r NaCl, 6 r CaCO3h 0.5 ZnSO4 · r 7Hž0. Ha 2500 μπ nHTaTenbHoň cpenbi πρη6ββπημτ 1 μπ βΗΤβφροΗβb xanecTBe neHoracHTenn Ha chhhkohoboé ocnoBe. KncnoTHocTb Nepen erepHnHaa-HHeň ημθθτ 3HaneHHe pH 6.0. Ilocne cTepnnHaanHH sánonHeHHoro 250 μπ KynbTy-panbHoft WHflKocTH φβρΜθΗΤΗΤορβ NPH 121C b aBTOKnaBe nocnenywmero oxnaatfle h hhh amino-30C cTapTÓByw KHcnoTHOCTb ycTaHaBnHBaioT OT pH 8.4 pH 6.8 Ha, NPH SaBnaa acenTHnecKyM noSaBKy 1 0% -hoé CTepHnbHOÉ cepHoň κηοποτμ. 3aceHHHbieφβρΜβΗτβηκοΗΗΜβ cPen HHKyčHpyioT b TeneHHe 168 nacoB npn TeMnepaType 3O0C hckopocth aapanHH 2500 μπ BO3nyxa b MHHyry πρκ nepeMenruBaHHH c ηηοϊομ oCopo-tob 800 o6 / mHh, CnycTH 1.5 uaca nocne aacesa ob Π opa Π Π Π ob ob ob ob ob ob ob ob ob ob ob ob ob ob Ha Ha Ha Ha ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ,8 ecnen 3a bthm pacTBop πρΗδβΒππΜτ b TeneHHe 21 naca what CKopoc-TbM HO3HpOBaHHH 3.5 Mn / naC. Bo ΒρβΜΗ φβρΜβΗΤβΗΗΗ Β acenTHHeCKHX ycnOBHHX tie-pHonHnecKH 6epyT npo6w KynbTypanbHOň jkhhkocth η b HHX ΜβτοηοΜ n * «l ^ y3HH barap c Hcnonb3OBaHHeM MHKpoopraHH3Ma Bacillus" subtilis ATCC 6633 b KanecT-Be flow-MHKpoopraHH3Ma οπρεηεππιοτ conepxaHHe Hóyp3eoTpHnHH-ochob3hhh, rnwKO 3 w aMMOHHííHoro a3OTa h TBepnwx Bewec.TB. napaMeTpw ρθ £ pH £ h conepxaHHH C0 β οτχοηππιΗΧ H3 annapaTa ra3OB KOHTponHpywT β χοηθ nponecca ΜετοηοΜ H3Mepe HHH-c perHCTpanHeň, CHHweHHe 3HaneHHH κηοποτηοοτη KynbTypanbHoÉ khhkocthHH »e SHaueHHH pH5.8 npenoTBpamaioT npHbaBneHHeM cTepHnhHoro 5% -ηογο pacTBO PA Enkor Harp. fapH CHHDKeHHH KOHneHTpanHH ΓΠΜΚΟ3Μ FIO 5 r / π ΗΠΗ aMMOHHÉHOrO a3OTa ΠΟ 0.5 r / π, HTO OTpaacaeTcn Ha yBenHneHHe H3MepeHHbix 3HaneHHÉ pH η ρθ · ι »and Taras Ha CHH-xeHHH conepacaHHH C02 β οτχοπηιηηχ ra3ax, πρΗδβΒππιοτ nepnonunecKH 25 r γπιοκο- 3W ΗΠΗ .10 Γ (NH4) 2SO4 BHfle KOHneHTpHpOBaHHblX CTepHHbHblX paeTBOpOB. Ilocne npeKpameHHH φβρΜβΗτβΑΗΗ B KynhTy-panbHoň xhakocth npucyTCTByioT eme ηβτκο o6HapyxHBaeMbie KonnuecTBa rn »KO3bi haMMOHHH. KOHneHTpannu HOyp3eOTpHUHH-OCHOBaHHH, OnpeflejjeHHbie B (JjHJIbTpaTaXxynbTyp oCohx pasjíHMHbix <J> epMeHTaitfiOHHbix nponeccoB, πρΗΒβΑβΗΜ b TaOnHue 1.2) JlHO $ HJiH9HpoBai «n> In Ha iTHOKoso-xejiaraHe KOHcepBHpoBaHHbiň μμαβαηΚ (EEC 6pyT-to KOHcepBHpoBaHHoro coflepwHMoro - οκοπο 300 μγ) HaaBaHHoro b npHMepe 1Hoyp3eoTpHUHHOo6pa3ywmero urraMMa ετρβπτοΜΗηβτοΒ cycneHAHpyioT C3 μπ 0,9Z-Horo pacTBopa xnopHCTóro HaTpHfl 0.5 μπ 3ton cycneHSHH cnyxHT b xanectBeHHOKynioMa απη 400 μπ cpeflbí npeflBapHTenbHoro KynbTHBHpoBaHHH nepBoň CTaAHH.Cpe # and npeflBapHTenbHoň xynbTypbi ΗΜββτ τοτ xe caMbiň cocTae η KHcnoTHocTb, htob npHMepe 1, OTy cpefly 3anHBaior b 3aKptiBaeMbie eaTHOn προδκοΗ uiHpoKoropnbieCyTbinxH eMKoeTb »2500 μπ b KonHnecTBax no 400 μπ. CTepwiHsaAHio npoBOflHT35-MHHyTHbiM HarpeBOM and 121 ° C b βΒτοκπβΒβ.
OxnaxfleHHbie onnTb ao κομηβτηοη TeMnepaTypbi h 3ace«HHbie xynbTypbi HHKyČHpyioTb τβΗβΗΗβ 48 MacoB npH 29°C ha Kananxe (180 o6/mhh). HcnonbsyioT 1200 μπ no-nyneHHoň tbkhm oÓpasoM xynbTypanbHoň xhakocth nepeoft npenBOPHTenbHoň xynbTy-pbi, Ha BTOpoň οτάπΗκ npenBapHTenbHoro xynbTHBHpoBaHHH aaceBanr 150 πκτροΒTepMocTepnnn3OBaHHoň cpenbi (β TeneHHe 60 μηη πρκ 121°C) Taxoro xe cocTaea,xax Ha nepBoň cthahh npeABapHTenbnoro KynbTHBHpoBaHHH, conepxameftcH β φβρ-ΜβΗΤβτορβ H3 cnenHanbHoň BbicoKOKanecTBeHHofi ετβπΗ (o6xnft oÓteM - 250 πητ-poe), ocHameHHOM ycTpoňcTBaMH απη ποΑβηκ CTepunbHoro BOSAyxa η nepeMemHBaio-mHM ycTpoňcTBOM. KynbTHBnpoBaHne προβοαητ npH 27-29°C, ckopocth pa6oTW nepe-MemHBawmero ycTpoňcTBa 240 ο6/μηη h ckopocth ποαοηη BosAyxa 1 πκτρ Ha πΗτρKynbTypanbHOft xhakocth b MHHyTy. /VlH OCHOBHOň KynbTypbl MCnonbSyiOT 4>epMeHTaTOpbI H3 CneAHanbHOň BblCOKOKaHeCT“BeHHoft cTann (o6ihhH o6teM -710 πκτροβ), ocHameHHbie ycTpoňcTBaMH απη noffannCTepunbHoro BosAyxa h pacnonoxeHHbíM b ueHTpe annapaTa nepeMemHBanmHM ycTpoň-ctbom, xoTopbie sanonHeHbí 500 nHŤpaMH nHTaTenbHOň cpeAM KaxAbiň (penenTypy cm.npHMep 1). B xanecTBe neHoracHTenn ηρΗθβΒΠΗίοτ 0,5 r/n aHTa$poiia. KncnoTHOCTbycTaHaBHHBaioT nepefl cTeptuiHaaiuieň na SHaqeHHe b npenenax pH 6,7-7,0. Οτβρκ-nHsaAHio nHTaTenbHOň cpeAU (6ea KOMnoHeHTa rnioKOSbi) προβοαητ Ha MecTe npeA“BapHTenbHbíM noAorpeBOM napoM pyCaniKH npHMepHO n° 70°C h nocnenyioiUHM Ha- -rpeeoM ocTpbiM napoM ao 12t°C b TeqeHHe 15 MHHyr. Ilocne oxnaxACHHH ao τβΜΠβ-paTypbi οκοπο 30°C ηρΗββΒΠΗίοτ b acenTiwecKHX ycnoBHHX rnwKoay b BHfle 50%-ho-γο βοαηογο pacTBopa, pa3AenbHO npocTepunHaoBaHHoro 30-MHHyTHNM HarpeeoM ao121°C. ΠβρβΑ noceeoM πρπ πομοπιη cTepnnbHoft 10%-Hoň cepHoň KHcnoTbi cHHxaioTCTapTOByio KHcnoTHocTb pH 8,6 a° 3Haqe«HH B npenenax pH 6,9-7,1. .OxnaxfleHHbie onnTb and TeMnepaTypbi h 3ace «HHbie xynbTypbi HHKyČHpyioTb τβΗβΗΗβ 48 MacoB npH 29 ° C ha Kananxe (180 o6 / mhh). HcnonbsyioT 1200 μπ no-nyneHHoň tbkhm oÓpasoM xynbTypanbHoň xhakocth nepeoft npenBOPHTenbHoň xynbTy PBI-Ha BTOpoň οτάπΗκ npenBapHTenbHoro xynbTHBHpoBaHHH aaceBanr 150 πκτροΒTepMocTepnnn3OBaHHoň cpenbi (β TeneHHe 60 μηη πρκ 121 ° C) Taxoro cocTaea xe, x and x Ha nepBoň cthahh npeABapHTenbnoro KynbTHBHpoBaHHH, conepxameftcH β φβρ- 3βΗΤβτορβ H3 cnenHanbHoň BbicoKOKanecTBeHHofi ετβπΗ (o6xnft oOteM - 250 πητ-poe), ocHameHHOM ycTidedcTBaMH απη ποΑβηκ CTepunbHoro BOSAyxa η nepeMemHBaio-mHM ycTtvcTBOM. KynbTHBnpoBaHne προβοαητ npH 27-29 ° C, pa6oTW paeo pWe not-MemHBawmero ycTwo cTBa 240 ο6 / μηη h ckopocth ποαοηη BosAyxa 1 πκτρ Ha πΗτρKynbTypanbHOft xhakocth b MHHyTy. / Humid OCHOBHOň KynbTypbl MCnonbSyiOT 4> H3 epMeHTaTOpbI CneAHanbHOň BblCOKOKaHeCT "BeHHoft cTann (o6ihhH o6teM πκτροβ -710) ocHameHHbie ycTpoňcTBaMH απη noffannCTepunbHoro BosAyxa h pacnonoxeHHbíM b ueHTpe annapaTa nepeMemHBanmHM ycTpoň-ctbom, xoTopbie sanonHeHbí 500 nHŤpaMH nHTaTenbHOň CPEA KaxAbiň (penenTypy cm.npHMep 1 ). B xanecTBe neHoracHTenn ηρΗθβΒΠΗίοτ 0.5 r / n aHTa $ poiia. The THOCTbycTaHaBHHBaioT nepepl cTeptuiHaaiuin on SHaqeHHe b npenenax pH 6.7-7.0. Peτβρκ-nHsaAHio nHTaTenbHOn cpeAU (6ea KOMnoHeHTa rnioKOSbi) προβοαητ Ha MecTe npeA “BapHTenbHbiMaAppeBOM napoM pyCaniKH npHMepHO n ° 70 ° C h nocnenyioiUHM Ha-rpeeoM ocTpbiM napoM o 12t ° C b TeqeHHe 15 MHHyr. Ilocne oxnaxACHHH and β-β-paTypbi 30 ° C ηρΗββΒΠΗίοτ b acenTiwecKHX ycnoBHHX rnwKoay b BHfle 50% -h-γοβοηο pacTBopa, pa3AenbHO npocTepunHaoBaHHoro 30-MHHyTHNM HarpeeoM o121 ° C. CTepnnbHoft 10% -Hoň cepHoň KHcnoTbi cHHxaioTCTapTOByio KHcnoTHocTb pH 8.6 and 3Haqe HH B npenenax pH 6.9-7.1. .
OepMeHTanHOHHbie cpeAbi ochobhoH KynbTypbl, saceflHHbie κβχηβΗ 5Z HHOKynioMa bto-poň cTaflHH npeAsapHTenbHoro KynbTHBHpoBaHHH, KynbTHBHpywr b TeneHHe 120 narcob npH TeMnepaType b npenenax 28-30°C h co CKopocTb» nonann BosAyxa · 500 πκτροΒ b MHHyTy (AaBneHue 0,14-0,16 Míla) npH nepeMemHBaHHH (320o6/mhh). K OAHoň H3 AByx xynbTyp 'φερΜβΗτβτορβ, HannHaa co 2-ro naca H κοηηηη 32-mqacoM nocne saceea, πρη63βπηιοτ npH nOMOUw nepHCTanbTHHecKÓro Hacoca-AOsaTo-pa c nocTQHHHoň cKopocTbio BOAHbďi pacTBOp, coAepxanwň 87 r/π KH^PO4, Tax mtoβ τβπβΗΗβ 30 uacoB Ha οαηη πητρ KynbTypbl πρΗδβΒΠΗβται 40 μγ φοεφβΤΗΟΓΟ P.OepMeHTanHOHHbie cpeAbi ochobhoH KynbTypbl, saceflHHbie κβχηβΗ 5Z HHOKynioMa BTO PON cTaflHH npeAsapHTenbHoro KynbTHBHpoBaHHH, KynbTHBHpywr b TeneHHe 120 narcob NPH TeMnepaType b npenenax 28-30 ° C h as CKopocTb »nonann BosAyxa · 500 πκτροΒ b MHHyTy (0.14-0.16 AaBneHue Mile) npH nepeMemHBaHHH (320o6 / mhh). The OAHoň H3 AByx xynbTyp 'φερΜβΗτβτορβ, HannHaa CO2-ro NACA H κοηηηη 32 mqacoM nocne saceea, πρη63βπηιοτ NPH nOMOUw nepHCTanbTHHecKÓro Hacoca-AOsaTo-pa c nocTQHHHoň cKopocTbio BOAHbďi pacTBOp, coAepxanwň 87 r / π KH ^ PO4, Tax mtoβ τβπβΗΗβ 30 uacoB Ha οαηη πητρ KynbTypbl πρΗδβΒΠΗβται 40 μγ φοεφβΤΗΟΓΟ P.
Bo BpeMH φβρΜβΗΤΒΑΗΗ b acenTHHecKHX ycnoBHHX nepHOAHHecKH 6epyT npoCbi xynb-TypanbHoň xhakocth η β hhx μθτοαομ αβΦΦυ3ηη b arap c HcnojibSOBaHHeM MHKpo-opraHH3Ma Bacillus subtilís ATCC 6633 b KanecTBe TecT-MHKpoopraHHSMa οπρβΑβ-HHIOTj KOHAeHTpaAHlO HOyp3eOTpHAHH“OCHOBaHHH H COnepXaHHe rn»KO3bl, aMMOHHftHOrO 258414 6 a3OTa η TsepflbK BemecTB. flapaMeTpbi pH h conepxaHHH C02 b otxoahdihx rasaxKOHTpojiHpywr b xofle nponecca Η3ΜβρβΗΗβΜ c perHCTpanHeň. ΠρΗ na,neHHH KOHueHTpauHH rmoxosbi mane 5 r/n wm ημμοηββΚηογο asoTa hhxs0,5 r/n, ΗΤΟ BHflHO ΠΟ HapaCTaHHSO 3HaUeHHň pH H CHHXeHHW COflepMaHHH C02 B OT-xoahiuhx rasax, nepHOAHuecxH ηρΗββΒΠΗίοτ 5000 r rnwxoabi ηπη 1800 r cy.nb<t>aTaaMMOHHH B BHfle ΚΟΗΑβΗΤρΗρΟΒβΗΗΟΓΟ CTepHJIbHOro' ΒΟΑΗΟΓΟ paeTBOpa. |B Apyryw xynbTypy φβρΜβΗτβτορβ φοςφβτβ, rmoxoay ηπη ονπΒφβτβ ημμοηηη Ηβ no-6aBJiHioT. PeryriHpoBaHHe 3HaneHHH pH ne ocymecTBnneTCHj HenpepbiBHo HSMepHeMue3Η3ΗΘΗΗΗ KHCJIOTHOCTH ΠβΧβΤ B Πρββ&ΠβΧ pH 6,4“7,2» B ΜΟΜβΗΤ npeKpameHHH φβρ-MeHTaijHH b xyjibTypanbHOň xhakocth copepxHTcn eme 6onee 5 r/n rnroxosbt h0,5 r/n aMMOHHňHoro ·β3οτβ.Bo BpeMH φβρΜβΗΤΒΑΗΗ b acenTHHecKHX ycnoBHHX nepHOAHHecKH 6epyT npoCbi xynb-TypanbHoň xhakocth η β HHX μθτοαομ αβΦΦυ3ηη b Arap c HcnojibSOBaHHeM MHKpo-opraHH3Ma Bacillus subtilis ATCC 6633 b KanecTBe flow-MHKpoopraHHSMa οπρβΑβ-HHIOTj KOHAeHTpaAHlO HOyp3eOTpHAHH "OCHOBaHHH H COnepXaHHe rn» KO3bl, aMMOHHftHOrO 258414 6 a3OTa η TsepflbK BemecTB. flapaMeTpbi pH h conepxaHHH C02 b otxoahdihx rasaxKOHTpojiHpywr b xofle nponecca Η3ΜβρβΗΗβΜ c perHCTpanHen. ΠρΗ on, neHHH KOHueHTpauHH rmoxosbi Mane 5 R / N WM ημμοηββΚηογο ASOT hhxs0,5 r / n, ΗΤΟ BHflHO ΠΟ HapaCTaHHSO 3HaUeHHň pH H CHHXeHHW COflepMaHHH C02 OT-B xoahiuhx rasax, nepHOAHuecxH ηρΗββΒΠΗίοτ 5000 R 1800 R rnwxoabi ηπη cy.nb <t > aTaaMMOHHH B BHfle ΚΟΗΑβΗΤρΗρΟΒβΗΗΟΓΟ CTepHJIbHOro 'ΒΟΑΗΟΓΟ paeTBOpa. | B Apyryw xynbTypes φβρΜβΗτβτορβ φοςφβτβ, rmoxoay ηνπΒφβτβ ημμοηηη Ηβ no-6aBJiHioT. PeryriHpoBaHHe 3HaneHHH pH not ocymecTBnneTCHj HenpepbiBHo HSMepHeMue3Η3ΗΘΗΗΗ KHCJIOTHOCTH ΠβΧβΤ Πρββ B & ΠβΧ pH 6.4 "7.2» B ΜΟΜβΗΤ npeKpameHHH φβρ-b MeHTaijHH xyjibTypanbHOň xhakocth copepxHTcn EME 6onee 5 r / n rnroxosbt H0.5 r / n · aMMOHHňHoro β3οτβ.
KoímeHTpaixHB Hoyp3eoTpHAHH-ocHosaHHfl, onpeneneHHbie b φκπΒτρβτβχ xynbTyp o6e-thx pasntiHHbix φβρΜβΗΤβΑΗή, npHBe.fleHbí b TaSjínne 2. 3) ΠΗθφΚΠΗ3ΗρθΒβΗΗΗΗ Ha ΓΠΙΟΚΟβΟ-ΧβΠβΤΗΗβ KOHCepBHpOBaHHblft MHyennň (Bec 6pyT-to KOHcepBHpoBaHHoro coaepxHMoro - οκοπο 300 μγ) cenexTaHTa HoypseoTpHAHHo-•oOpasyiomero nrraMMa IMET JA 3890 b o6pa6aTbiBaioT b OTHomeHHH npenBapHTenbHO-ro KynbTHBHpoBaHHH, xax onncano b npHMepe 2. Λπη ocHOBHoň KynbTypw Hcnonb3yi0T naCopaTopHbiň φερΜβΗΤβτορ oSnpiM ο6βθμομ 7 π,ocHanjeHHbiň ycTpoňcTB8MH απη noflaMH cTepjunbHoro Boanyxa h pacnonoxeHHMM bneHTpe nepeMeuiHBaromHM ycTpoňcTBOM, κοτορωή aanonHHioT 4,5 π nHTaTenbHoň cpeam.KoímeHTpaixHB Hoyp3eoTpHAHH-ocHosaHHfl, onpeneneHHbie b φκπΒτρβτβχ xynbTyp o6e-THX pasntiHHbix φβρΜβΗΤβΑΗή, npHBe.fleHbí b TaSjínne 2, 3) ΠΗθφΚΠΗ3ΗρθΒβΗΗΗΗ Ha-ΓΠΙΟΚΟβΟ ΧβΠβΤΗΗβ KOHCepBHpOBaHHblft MHyennň (BEC 6pyT-to KOHcepBHpoBaHHoro coaepxHMoro - οκοπο 300 μγ) cenexTaHTa HoypseoTpHAHHo- • oOpasyiomero nrraMMa IMET JA 3890 b b o6pa6aTbiBaioT OTHomeHHH npenBapHTenbHO-ro KynbTHBHpoBaHHH, XAX onncano b npHMepe second Λπη ocHOBHoň KynbTypw Hcnonb3yi0T naCopaTopHbiň φερΜβΗΤβτορ oSnpiM ο6βθμομ 7 π, ocHanjeHHbiň ycTpoňcTB8MH απη noflaMH cTepjunbHoro Boanyxa h pacnonoxeHHMM bneHTpe nepeMeuiHBaromHM ycTpoňcTBOM, κοτορωή aanonHHioT 4.5 π nHTaTenbHoň CPEA.
KyAbTHBHpOBaHHe ΠΡΟΒΟΑΗΤ B ΜΟΑΗφΗΑΗρΟΒβΗΗΟΰ Cpefle (npHMep OCynteCTBneHHH I),coAepxameň 60 r/n rni0KO3bi. B xanecTBe neHoracHTenn πρκδβΒΠΗίοτ 0,5 r aHTa-φροΗβ NM-40 Ha ηκτρ KynbTypanbHoň xaakocth. βΠΗ MOnejlHpOBaHHH ΤΘΧΗΗΗβΟΚΗΧ yCHOBHň CTepHJIH3aAHlO ΠΡΟΒΟΑΗΤ B φβρΜβΗΤβΤΟρβH3 cneAnanbHoň BbicoxoxaHecTBeHHoň CTanH, sanonHeHHOM 2500 n nHTaTenbHoň cpe-Abi 6β3 γπιοκοβηογο KOMnoHeHTa. KHcnoTHOCTb ycTaHHBnuBaioT nepen CTepKAHaaimeňHa pH 7,4. CTepnnH3auHio προβοαητ Ha MecTe npeABapHTenbfibíM noAorpeeoM napoM.py6amxH po 75° C h nocnenyromHM HarpeBOM oerpbiM napoM no 120°C b τβΊβΗΗβ15 MHHyT·· Ilocnei oxnaxneHHH no 29°C npuCaBnHioT b acenTHuecxHX ycnoBHHX rnto-KO3y, paanenbHo npocTepHHH30BaHHyto b BHAe 50%-ηογο pacTsopa 30-MHHyTHhiM Ha-rpeBOM po 120°C. nepen aaceBOM ποΗΗΧβιοτ cTapTOByw KHcnoTHOCTb ot 3HaqeHHflpH 8,1 cepHoň KHcnoToň thkhm oOpasoM, hto6h cTapTOBoe SHaneHne pH cocTaenH-no nocne sacesa 7,4. OepMeHTanHOHHyio xynbTypy 3aceBaioT 4% HHoxynioMa BTopoftcTanHH npenBapHTenbHoro KynbTHBHpoBaHHH. flnn npoBeneHHH οπωτβ H3 φβρΜβΗτβ-τορβ nepeBOA«T 4,5 ň cpenbi b naSopaTopHbiň φβρΜβΗτβτορ, xoTopbiň CTepunnsyioTpaaflenbuo b He3anonneHHOM coctohhhh b aBTOKnaee.KyAbTHBHpOBaHHe ΠΡΟΒΟΑΗΤ B ΜΟΑΗφΗΑΗρΟΒβΗΗΟΰ Cpefle (npHMep OCynteCTBneHHH I), coAepxameň 60 r / n rni0KO3bi. B xanecTBe neHoracHTenn πρκδβΒΠΗίοτ 0.5 r aHTa-φροΗβ NM-40 Ha ηκτρ KynbTypanbHoň xaakocth. βΠΗ MOnejlHpOBaHHH ΤΘΧΗΗΗβΟΚΗΧ yCHOBH CT CTepHJIH3aAHlO ΠΡΟΒΟΑΗΤ B φβρΜβΗΤβΤΟρβH3 cneAnanbHoňn BbicoxoxaHecTBeHHoon CTanH, sanonHeHHOM 2500 n nHTaTenbHoň cpe-Abi 6β3 γπιοκοβηογο KOMnoHeHTa. KHcnoTHOCTb ycTaHHBnuBaioT nepen CTepKAHaaimeHa pH 7.4. CTepnnH3auHio προβοαητ Ha MECT npeABapHTenbfibíM noAorpeeoM napoM.py6amxH to 75 ° C h nocnenyromHM HarpeBOM oerpbiM drinks but 120 DEG C. b τβΊβΗΗβ15 MHHyT ·· Ilocnei oxnaxneHHH No 29 C b npuCaBnHioT acenTHuecxHX ycnoBHHX RNTO-KO3y, paanenbHo npocTepHHH30BaHHyto b BHAe 50% -ηογο pacTsopa 30-MHH-thiMH-rpeBOM at 120 ° C. non-aceBOM ποΗΗΧβιοτ cTapTOByw KHcnoTHOCTb ot 3HaqeHHflpH 8.1 cepHoN KHcnoToň thkhm oOpasoM, hto6h cTapTOBoe SHaneHne pH cocTaenH-no nocne sacesa 7,4. OepMeHTanHOHHyio xynbTypes 3aceBaioT 4% HHoxynioMa BTopoftcTanHH npenBapHTenbHoro KynbTHBHpoBaHHH. flnn npoBeneHHH οπωτβ H3 φβρΜβΗτβ-τορβ nepeBOA T T 4,5 c cpenbi b at SopaTopHbi φβρΜβΗτβτορ, xoTopbien CTepunnsyioTpaaflenbuo b He3anonneHHOM coctohhhh b aBTOKnaee.
OcHOBHyto xynbTypy BbipamHBatoT b TeneHHe 146 naco-B npH TeMnepaType 29°C h cko-pocTH aapauHH 4,5 n BO3nyxa b MHHyTy. Hauanbiioe uncno οδοροτοΒ nepeMeuiHBaro-ntero ycTpoňcTBa b 800 o6/mhh yBenHHHBaioT pnu ynymneHHH cHaSxeHHH xynbTypbiKHcnopOAOM; ηηηηηηη c 28-ro naca hhcao οδοροτοΒ MeiuanxH cocTaBnaeT1000 o6/mhh. Mexny 2-m h 40-m nacaMH nocne 3aceBa npH πομοπιη nepHCTanbTH-Hecxoro Hacoca πρκββΒΠΗίοτ 784 μγ KH2P04 b BHAe βοαηογο pacTBopa c paaHoňcxopocTbH) nonanH b npenenax 1,25-1,75 μγ φοοφβΤΗΟΓο P b uac h Ha πητρ Kynb-TypanbHoň xhakocth. ABTOMaTHnecxoň nonaneň 25Ζ-ηογο βοαηογο pacTBopa aMMHa—xa o6ecneHHBaioT rBOnnepxaHHe perynHpyeMoro 3HaneHHH Ha ypoBHe He Meóee pH6,2 η, τβΜ caMbiM, AocTaTOHHoe cHá6xeHHe xynbTypbi aMMOHHňHbíM a3OTOM. Γπιοκο-ay πρΗββΒΠΗίοτ nepHOAHHecxH b BHne KOHueHTpHpoBaHHoro βοαηογο pacTBopa Bcer-pa Torna, Korná pe3ynbTaTbi aHannsoe, npoBOHHMbix no xony φερΜβΗΤβΑΗΗ, noxa-3biBawT naneHHe κοηαθητρβαηη rmoxosbi HHxe 10 r/n. Bo epeMH φβρΜθΗΤβηΗΗ b 258414 7 acerrrHHecKHx ycnosHHx nepnoxumecKn 6epyT προ6ω xynbTypanbHoft khxxkocth h b«frwibTpaTax KynbTypw οπρβχχεηΗΐοτ KOHyeHTpawuo Hoyp3eoTpniínH—ocHOBaHHH Μετο/χοΜΑΗφφγ3ΗΗ b arap (TaCnHixa 3). 4) B npoixecce φερΜεκτβιχηΗ, npoBOflHMOM aHanornnHO npHMepy ocymecTBJíeHHH 3),fíonn cynbýaTa nHHxa cpeflbí ochobhoíí xynbTypbi aaMeHjnoT Ha 0,2 r/n FejíSO,,)^ *9 HjO. KoHixeHTpaixHH HoypaeoTpHiXHHa b φΗηητρβτε xynbTypbi cocTaBJíHeT nocne 120-uacoBoň φερΜβΗτβιχΗΗ 8200 μκγ/mji (TaGnnxxa 4). 5) B npoixecce φερΜβΗτειχΗΗ, προβοαημομ aHanornwHO npHMepy ocymecTBneHHH 3),HipHHHtoynynynynynambbbb byenebbe 146bbbbbbH Te 146 146epa 146 146 146 146 146-146 146 146 146 146 146 146 146MMMMMMMMMMMMMM ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° ° °. Hauanbiioe uncno οδοροτοΒ nepeMeuiHBaro-ntero ycTm3cBb b 800 o6 / mhh yBenHHHBaioT pnu ynymneHHH cHaSxeHHH xynbTypbiKHcnopOAOM; ηηηηηηη c 28-ro naca hhcao οδοροτοΒ MeiuanxH cocTaBnaeT1000 o6 / mhh. 2-m h 40-m nacaMH nocne 3aceBa npH 784 μg KH2P04 b BHAe βοαηογο pacTBopa c paaHoňcxopocTbH nonanH b npenenax 1,25-1,75 μγ φοοφβΤΗΟΓο P b uac h Ha πητρ Kynb-TypanbHoň xhakocth. ABTOMaTHnecxon nonaneň 25Ζ-ηογο βοαηογο pacTBopa aMMHa — xa6xneHHBaioT rBOnnepxaHHe perynHpyeMoro 3HaneHHH Ha ypoBHe He Meoee pH6,2 η, τβΜ caMbiM, AocTaTOHHoe cH6xeHHe xynbTypbi andMMOHHbHM x3TOM. HOπιοκο-ay πρΗββΒΠΗίοτ nepHOAHHecxH b BHne KOHueHTpHpoBaHHoro βοαηογο pacTBopa Bcer-pa Torna, Korna pe3ynbTaTbi and Hannsoe, npoBOHHMbix no xony φερΜβΗΤβΑΗΗ, noxa-3biBawT naneHHe κοηαθητρβαηη rmoxosbi HHxe 10 r / n. Bo epeMH φβρΜθΗΤβηΗΗ b 258414 7 acerrrHHecKHx ycnosHHx nenoxumecKn 6epyT προ6ω xynbTypanbHoft khxxkocth h b «frwibTpaTax KynbTypw KOHyeHTpawuo Hoyp3eoTpniinH — ocHOBaHHH /ετο / χοΜΑΗφφγ3ΗΗ b arap (TaCnHixa 3). 4) B npoixecce φερΜεκτβιχηΗ, npoBOflHMOM and ornnnnnnnnnpHMepy ocByecnHHH 3), cynblytin and nHHxa cpeflbi is xynbTypbi aMeHjnoT Ha 0.2 r / n Fe 1 SO 4 · 9 9 H 10. KoHixeHTpaixHH HoypaeoTpHiXHHa b φΗηητρβτε xynbTypbi cocTaBJíHeT nocne 120-uacoBoň φερΜβΗτβιχΗΗ 8200 μκγ / mji (TaGnnxxa 4). 5) B npoixecce φερΜβΗτειχΗΗ, προβοαημομ aHanornwHO npHMepy ocymecTBneHHH 3)
flOJiío cynbftaTa ιχηηκβ aaMenjnoT Ha 0,13 r/n A12(SO4) 18 H20. KoHtxeHTpaixHRflNiCe cAnBftaTa ιχηηκβ aaMenjnoT Ha 0.13 r / n A12 (SO4) 18 H 2 O. KoHtxeHTpaixHR
HoypaeOTpHUHHa b φπηΒτρβτβ xyjibTypbi cocTaBJíaeT nocne' 120-nacoBoň φερΜβΗτβ-ixhh 8400 μχγ/μπ (TaSjíHna 4). 6) B flajibHeftmeň φβρΜβΗτβιχΗΗ, b 3HanHTenbHoň CTenenu npoBORHMoft aHanoruHHOnpHMepy ocymecTBJíeHHH 5), xonHwecTBO coflepxanxerocn b nHTaTenbHoň cpene o6ea-MacjienHoro coeBoro nipoTa CHHxatoT ot 11 r/n ρ,ο 4 r/n» flanee oHaneHHe pH cpenuycTaHaBnusaioT nepea cTepHnH3aixHeň pa36aBneiiHUM boahmm pacTBOpoM enxoro Har-pa Ha pH 8,1« Ilocne cTepunHaaxxHH napoM non naeneHHeM 3HaueHHc pIlycTaHaertH“aaioT KOHneHTpHpOBaHHoň cepHoň xHcnoToň TaxHM o6pa3OM, htoGm oho cocraBnanopH 7,2 nocne sacéBa. Φοοφβτ npn6aBnHtoT Mexny 2-m h 72-m nacaMH πρκ πομοπχηβοηηογο pacTBopa KH2P0^, xoTopwň ΠρΗββΒΠΗΙΟΤ C nOCTOHHHOň CXOpOCTb» T8XHM o6-paaoM, HToCbi Gbino oGecneneHO cHaGxeHHe φοοφβτοΜ b xonHnecTBe 1,4 μγ φοεφβτ-ηογο P b sac h Ha πητρ xynbTypanbHoň jkhxxxocth. B npoixecce φβρΜβΗΤΗΐχΗΗ rnioxo-ay nepHOAHnecKH ηρΗββΒηΗΐοτ tbkhm o6pa3OM, htoGm χοιπχεΗτ pannu γπιοκο3μ He chh-3HJiaCb HHXe 1 r/n. 3ΗβΊβΗΗ6 pH nOXmepMHBaiOT ΤΗΤρΟΒβΗΗβΜ 25%-HMM pacTBOpoMTHAPOOKMCH HMMOHHHHaypOBHe pH 6,0. IIonynaeMbie χοΗηβπτρβιχΗΗ HoypaeoTpHixHHa npHBenenbi b TaGnHixe 5. 1HoypaeOTpHUHHa b φπηΒτρβτβ xyjibTypbi cocTaBJíaeT nocne '120-nacoBoň φερΜβΗτβ-ixhh 8400 μχγ / μπ (TaSjíHna 4). 6) B flajibHeftmeň φβρΜβΗτβιχΗΗ b 3HanHTenbHoň CTenenu npoBORHMoft aHanoruHHOnpHMepy ocymecTBJíeHHH 5) xonHwecTBO coflepxanxerocn b nHTaTenbHoň cPen o6ea-MacjienHoro coeBoro nipoTa CHHxatoT rt 11 r / n ρ, ο 4 r / n »flanee oHaneHHe pH cpenuycTaHaBnusaioT NEP cTepHnH3aixHeň pa36aBneiiHUM boahmm pacTBOpoM enxoro Har -pa Ha pH 8.1 Ilocal cHepcHaaxxHH for non-naphthaHHeM 3HaueHHc pIlycTaHaertH "and thioTHeHHePoPeNHeCoNHeNHyxHoOn TaxHM o6pa3OM, htoGm oho cocraBnanopH 7,2 nocne suaBa. Φοοφβτ npn6aBnHtoT Mexny m-2 h-72 m nacaMH πρκ πομοπχηβοηηογο pacTBopa KH2P0 ^, C xoTopwň ΠρΗββΒΠΗΙΟΤ nOCTOHHHOň CXOpOCTb »T8XHM O6-paaoM, HToCbi Gbino oGecneneHO cHaGxeHHe φοοφβτοΜ b xonHnecTBe μγ φοεφβτ 1,4-b ηογο P h SAC Ha πητρ xynbTypanbHoň jkhxxxocth. B npoixecce φβρΜβΗΤΗΐχΗΗ rnioxo-ay nepHOAHnecKH ηρΗββΒηΗΐοτ tbkhm o6pa3OM, htoGm χοιπχεΗτ pannu γπιοκο3μ He chh-3HJiaCb HHXe 1 r / n. 3ΗβΊβΗΗ6 pH nOXmepMHBaiOT ΤΗΤρΟΒβΗΗβΜ 25% -HMM pacTBOpoMTHAPOOKMCH HMMOHHHaypOBHe pH 6.0. IIonynaeMbie χοΗηβπτρβιχΗΗ HoypaeoTpHixHHa npHBenenbi b TaGnHixe 5. 1
0opMyna HaoGpeTeHHH0opMyna HaoGpeTeHHH
CnocoG φβρΜβΗΤβτΗΒΗΟΓΟ nonyneHHB HoypaeoTpHixHHa nyTeM ycTaHOBXH χοηιχβΗΤρβιχΗΗOHonornnecKH flOCTynHoro φοοφβτβ, oTnHuátonxHňcn τ e μ, ητο Mepó-πρΗΗΤΗΗ ηρΗΗΗΜβιοτ B OTflenbHOCTH Hnu b χομΘηηβιχηη TaKHM oGpaaoM, hto - CTepHnuaanH»'nHTaTenbHbix cpen προΒο/χητ βοαηημμ napoM non naaneHHeM bnpMcyTCTBHH hohob MeTannoB, oGpaayioaxHX c φοοφβτοΜ τργχχΗσ pacTBopHMweocaflKH, nocne npenmecTByiomeň ycTaHOBXH xhchothocth ηητβτβηΒΗωχ cpen HasHaneHHfl pH 7 - pH 9 h/ioth onHOBpeMeHHoro npHcyrcTBHS hohob cyinMfcaTa hxapGoHaTa xanbixHH, - coflepxaHHe φοεφΒτεοχχβρχβηχΗΧ xoMnnexcHbK cyGcTpaTOB b nHTarenbHwx cpeftaxcHHxatoT nepen cTepHnHaanneň, - nnn npenoTBpameHHH n&nbHeňniHx ηκΜΗΤβιχΗΗ φοοφβτ πρκββΒηηιοτ b HananeφΗΠΒΤρβΧΧΗΗ Η/ΗΠΗ BO ΒρβΜΗ flanbHeňlUeň φβρΜβΗΤΒΧΧΗΗ. K Heft τβθηΗίχω Ha 5-η cTpaHnqax 258414 8CnocoG φβρΜβΗΤβτΗΒΗΟΓΟ nonyneHHB HoypaeoTpHixHHa rivet ycTaHOBXH χοηιχβΗΤρβιχΗΗOHonornnecKH flOCTynHoro φοοφβτβ, oTnHuátonxHňcn τ e μ, ητο MEPO-πρΗΗΤΗΗ ηρΗΗΗΜβιοτ B OTflenbHOCTH HNU b χομΘηηβιχηη Takhte oGpaaoM, HTO - CTepHnuaanH » 'nHTaTenbHbix cPen προΒο / χητ βοαηημμ beverages non naaneHHeM bnpMcyTCTBHH hohoba MeTannoB, oGpaayioaxHX c φοοφβτοΜ τργχχΗσ pacTBopHMweocaflKH, night npenmecTByiomeň ycTaHOBXH xhchothocth ηητβτβηΒΗωχ cPen HasHaneHHfl pH 7 - pH 9 h / IOTH onHOBpeMeHHoro npHcyrcTBHS hohoba cyinMfcaTa hxapGoHaTa xanbixHH - coflepxaHHe φοεφΒτεοχχβρχβηχΗΧ xoMnnexcHbK cyGcTpaTOB b nHTarenbHwx cpeftaxcHHxatoT Nepen cTepHnHaanneň - NNN npenoTBpameHHH n & nbHeňniHx ηκΜΗΤβιχΗΗ φοοφβτ πρκββΒηηιοτ b HananeφΗΠΒΤρβΧΧΗΗ Η / ΗΠΗ BO ΒρβΜΗ flanbHeňlUeň φβρΜβΗΤΒΧΧΗΗ. K Heft τβθηΗίχω Ha 5-η cTpaHnqax 258414 8
Ta6ji«na 1 ·Ta6ji «to 1 ·
CoaepxaHHe HoypaeoTpinjHHa b KynbTypaxuiTaMMa Streptomyces noursei IMER JA 3890b--//NG 13-14 c floSaBKaMH KH^PO^ rn»KO3M h(NH4)7804 h 6ea noGasoK b JiaCopaTopHOMφβρΜβΗΤβΤΟρβ B 3a8HCHMOCTH OT ΒρβΜβΗΗφβρΜβΗΤΗΗΗΗCoaepxaHHe HoypaeoTpinjHHa b KynbTypaxuiTaMMa Streptomyces noursei IMER JA 3890b - // NG 13-14 c floSaBKaMH KH ^ PO ^ rn »
BpeMfl φβρΜΘΗ- C floSaBKaMH Ses AO0aBOK (KOMTpOJIb) , TaUHH, M μκγ/μπ 1 2 μκγ/μπ 1 v 24 1035 249 415 100 48 2980 165 1805 100 72 5412 294 1840 100 96 8311 213 3905 100 120 8800 195 4520 100 140 9230 213 4005 100BpeMfl φβρΜΘΗ- C floSaBKaMH es A K K T OJ 2 2 2 2 1 2 μκγ / μπ 1 v 24 1035 249 415 100 48 2980 165 1805 100 72 5412 294 1840 100 96 8311 213 3905 100 120 8800 195 4520 100 140 9230 213 4005 100
Ta6nuua 2:Ta6nuua 2:
Co/xepxaHHe Hoyp3eoTpHUHHa b KynbTypaxniTaMMa Streptomyces noursei IMET JA 3890b--//NG 13-14 c fíoSaBKaMH KH2P04, rntoKOStí h(NH4)2S04 h 6ea floGaBOK β φβρΜβΗτβτορβ eM-KOCTblO 500 Π B aaBHCHMOCTH OT ΒρβΜβΗΗφβρΜβΗΤβμΗΗCo / xepxaHHe Hoyp3eoTpHUHHa b KynbTypaxniTaMMa Streptomyces noursei IMET JA 3890b - // NG 13-14 c fiSaBKaMH KH2P04, rntoKOSt h (NH4) 2S04 h 6ea floGaBOK β φβρΜβΗτβτορβ eM-KOCTblO 500 Π B aaBHCHMOCTH OT ΒρβΜβΗΗφβρΜβΗΤβμΗΗ
BpeMfl φερΜβΗ-TaUHH, fl C AoCaBKaMH Bea floóasoK (κοητροπβ). μκγ/μπ z μΚγ/ji 1 Z 20 225 216 104 100 44 1600" 228 702 100 68 4858 299 1627 100 92 4903 208 2362 100 1 16 6862 191 3585 100 144 7781 200 3885 100.BpeMfl εερΜβΗ-TaUHH, fl C AoCaBKaMH Bea flooasoK (κοητροπβ). μκγ / μπ z μΚγ / ji 1 Z 20 225 216 104 100 44 1600 "228 702 100 68 4858 299 1627 100 92 4903 208 2362 100 1 16 6862 191 3585 100 144 7781 200 3885 100.
Ta&mma 3:Ta & mma 3:
CoflepxaHHe HoypaeoTpmjHHab KyjibType ceneKTaHTanrraMMa Streptomyces nour-sei IMET JA 3890b c no&aB-K3MH ΚΗζΡ04> ΓΠ10ΚΟ3Μ H(NH+)2S04 β φβρΜεΗτβτορβeMKOCTb» 4,5 π b 3aBHcn-<MOCTH OT ΒρβΜβΗΗ φερΜβΗ-TayHHCoflepxaHHe HoypaeoTpmjHHab KyjibType ceneKTaHTanrraMMa Streptomyces nour-sei IMET JA 3890b c no & aB-K3MH ΚΗζΡ04> ΚΟΠ10ΚΟ3Μ H (NH +) 2S04 β φβρΜεΗτβτορβeMKOCTb »4,5 π b 3aBHcn- <MOCTH OT ΒρβΜβΗΗ φερΜβΗ-TayHH
BpeMfl φερΜβΗτβμΗΗ,q KoHueHTpanHH Hoyp-seoTpHUHHa, μκγ/μπ 1 1 20 320 44 2330 258414 9 ΠροηοπχβΗΗβ TaOji.3 _ i __ ......Ί_________?________ 68 6240 92 8010 116 11040 146 14070BpeMfl φερΜβΗτβμΗΗ, q KoHueHTpanHH Hoyp-seoTpHUHHa, μκγ / μπ 1 1 20 320 44 2330 258414 9 ΠροηοπχβΗΗβ TaOji.3 _ i __ ...... Ί _________? ________ 68 6240 92 8010 116 11040 146 14070
Ta6jiHqa 4:Ta6jiHqa 4:
CoflepxaHHe Hoyp3eoTpHHHHanpoBeaeHHoň φερΜεΗτβίφΗ bCOOTBeTCTBHH C ΠρΗΜβρΟΜocymecTBjíeHHH 4) c ηοηημηxene3a (XII) η φερΜβΗτβΐίΗΗB COOTBeTCTBHH C ΠρΗΜβρΟΜocymecTBjíeHHH 5) c ηοηβμηaniOMHHHH (III) B 3HBHCH“MOCTH OT BpeMeHH φβρΜβΗΤβ“UHHCoflepxaHHe Hoyp3eoTpHHHHanpoBeaeHHoň φερΜεΗτβίφΗ bCOOTBeTCTBHH ΠρΗΜβρΟΜocymecTBjíeHHH C 4) C ηοηημηxene3a (XII) η C φερΜβΗτβΐίΗΗB COOTBeTCTBHH ΠρΗΜβρΟΜocymecTBjíeHHH 5) c ηοηβμηaniOMHHHH (III) B 3HBHCH "OT MOCTH BpeMeHH φβρΜβΗΤβ" UHH
BpeMH φβρΜβΗ-TaUHH, M KoHqeHTpaijHH HoypaeoTpH-UHna b μκγ/mji no* npHMepyocymecŤBneHHH A) ] 5) 24 530 800 48 2500 3200 72 .5400 6300 96 7250 7400 120 8200 8400BpeMH φβρΜβΗ-TaUHH, M KoHqeHTpaijHH HoypaeoTpH-UHna b μκγ / mji no * npHMepyocymecbneHHH A)] 5) 24 530 800 48 2500 3200 72 .5400 6300 96 7250 7400 120 8200 8400
TaCnnua 5:TaCnnua 5:
CoflepjKaHHe Hoyp3eoTpnuHHaB KyjIbTypajIbHOH WHflKOCTHnaČopaTopHoro φερΜβΗτβτορΒc flo6aBKaMH ΚΗ2Ρ04, γπιοκο-3bl H THflpOOKHCH aMMOHHH,coflepxaujeň ηοηη λπιομηηηη(III), B aaBHCHMOCTH OTBpeMeHH φερΜβΗτβμΗΗCoflepjKaHHe Hoyp3eoTpnuHHaB KyjIbTypajIbHOH WHflKOCTHnaČopaTopHoro φερΜβΗτβτορΒc flo6aBKaMH ΚΗ2Ρ04, γπιοκο 3BL-H THflpOOKHCH aMMOHHH, coflepxaujeň ηοηη λπιομηηηη (III), B aaBHCHMOCTH OTBpeMeHH φερΜβΗτβμΗΗ
BpeMH φβρ- KoHqeHTpayHH HoypaeoTpHUH-MeHTauHH,H Ha, μκγ/μη 24 800 48 . . · 2000 72 5660 96 8900 • 120 11000 258414 10 ΑηΗΟΤΒΑΗΠBpeMH φβρ- KoHqeHTpayHH HoypaeoTpHUH-MeHTauHH, H Ha, μκγ / μη 24 800 48. . · 2000 72 5660 96 8900 • 120 11000 258414 10 ΑηΗΟΤΒΑΗΠ
HaoSpeTeHHe othochtch k nojiyqeHHio οτρθπτοτρηαηηοβογο 8Ητη6ηοτηκ3 HoypseoT-pniyuia ΜΗκροδΗοποΓΗΗβοκοίί (fcepMeHTaimeň πγτβΜ οκ33ΒΗηη BosfleňcTBHH Ha φοοφβτ-huA οβΜβΗ, Pfin 9τοή μβπκ ocymecTBnnioT flBe MaHHnyjiHUHH OflHy aa Apyroft κπηpa3A&ni>Ho: - cHHKeHHe HaqanbHoň κοΗΑβΗτρβΑΗΗ pacTBopuMoro h AocTyraoro φοεφβτβ ao hhs-Koro ypoBHa bo BpeMH CTepiuiHsaAHH nHTaTenbHoií cpeAbi * ' ’ » » - noBTopHoe npH.6aBjieHHe φοοφβτβ nyTeM ποΑβΗΗ φοεφβτεοΑβρχβπΐΗχ BentecTB b ποα-xoAHoteM peKHMe αοβηροβηηηη»HaoSpeTeHHe othochtch to nojiyqeHHio οτρθπτοτρηαηηοβογο 8Ητη6ηοτηκ3 HoypseoT-pniyuia ΜΗκροδΗοποΓΗΗβοκοίί (fcepMeHTaimeň πγτβΜ οκ33ΒΗηη BosfleňcTBHH Ha φοοφβτ-hua οβΜβΗ, Pfin 9τοή μβπκ ocymecTBnnioT flBe MaHHnyjiHUHH OflHy aa Apyroft κπηpa3A & Her> Ho - cHHKeHHe HaqanbHoň κοΗΑβΗτρβΑΗΗ pacTBopuMoro h AocTyraoro φοεφβτβ and HHS-Koro ypoBHa bo BpeMH CTepiuiHsaAHH nHTaTenbHoií cpeAbi * '' »» - noBTopHoe npH.6aBjieHHe φοοφβτβ nyTeM ποΑβΗΗ φοεφβτεοΑβρχβπΐΗχ BentecTB b ποα-xoAHoteM peKHMe αοβηροβηηηη »
CmcKeHne liauajibMoň KOHpeHTpamut AOCTynHoro φοοφβτβ προβοαητ nyreM yBenHHe-hhh 3naMeHHH pH bo BpeMH cTepunnsanHH η ocaxAeHHH φοοφβτοοηβρχβπΒίχ ocanKOBB npncyTCTBHH φόοφβΤΟΟβΧΑβΙΟΙίρΐΧ HOHOB. KHCJIOTHOCTb φερΜβΗΤΗΑΗΟΗΗΜΧ cpefl jih6oycTaHaBJíHBaioT bo BpeMH cTepnnn3annn npHMo Ha niejioHHbie SHaueHHH pH, λη6ο yae-AHHHBaioT aa cm6t npncyTCTBHH 6onee BbicoKHX KOHineHTpaAHň εγπΗφβτβ HapHAy CKapCoHaTOM xanbAHH bo BpeMH cTepunnaainHH ocTpbiM napoM, floSaBjíeHHe φοοφβτβ bφοοφΒτοοΑβρχβπξΗΚ BewecTBax προβοαητ no onTHManbHOMy pexHMy bo BpeMH φβρΜβΗ-TaimH c npeAOTBpameHHeM AajibHeňoiHx πημηΤ8αη6 ηπη αρυγηχ HeCnaronpHHTHMX yc“ JIOBHft. ΠρΗ3Η3Η0 Η3θ6ρ©ΤβΗΗβΜ ΠΟ peayjIbTaTaM 3KCnepTH3bl, OCymeCTBfieHHOft BeAOMCTBOMno AenaM Η3θ6ρβτβΗΗή h naTeHTOB TflP. - 258414CmcKeHne liauajibMoň KOHpeHTpamut AOCTynHoro φοοφβτβ προβοαητ nyreM yBenHHe-hhh 3naMeHHH pH bo BpeMH cTepunnsanHH η ocaxAeHHH φοοφβτοοηβρχβπΒίχ ocanKOBB npncyTCTBHH φόοφβΤΟΟβΧΑβΙΟΙίρΐΧ HOHOB. KHCJIOTHOCTb φερΜβΗΤΗΑΗΟΗΗΜΧ cpefl jih6oycTaHaBJíHBaioT bo BpeMH cTepnnn3annn npHMo Ha niejioHHbie SHaueHHH pH λη6ο Yae-AHHHBaioT aa cm6t npncyTCTBHH 6onee BbicoKHX KOHineHTpaAHň εγπΗφβτβ HapHAy CKapCoHaTOM xanbAHH bo BpeMH cTepunnaainHH ocTpbiM beverages floSaBjíeHHe φοοφβτβ bφοοφΒτοοΑβρχβπξΗΚ BewecTBax προβοαητ well onTHManbHOMy pexHMy bo BpeMH φβρΜβΗ-TaimH c npeAOTBpameHHeM AajibHeňoiHx πημηΤ8αη6 ηπη αρυγηχ HeCnaronpHHTHMX yc “JIOBHft. Πρθ3Η3Η0 Η3θ6ρ © ΗΗβΗΗβΜ ΠΟ peayjIbTaTaM 3KCnepTH3bl, OCymeCTBfieHHOft BeAOMCTBOMno AenaM Η3θ6ρβτβΗΗή h naTeHTOB TflP. - 258414
Claims (1)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DD83247370A DD233753A3 (en) | 1983-01-20 | 1983-01-20 | PROCESS FOR PRODUCING NOURSEOTHRICIN |
Publications (1)
Publication Number | Publication Date |
---|---|
CS258414B1 true CS258414B1 (en) | 1988-08-16 |
Family
ID=5544502
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CS84355A CS258414B1 (en) | 1983-01-20 | 1984-01-17 | Method of nourserotricine fermentation production |
Country Status (5)
Country | Link |
---|---|
JP (1) | JPS6062987A (en) |
BG (1) | BG49225A1 (en) |
CS (1) | CS258414B1 (en) |
DD (1) | DD233753A3 (en) |
SU (1) | SU1451165A1 (en) |
-
1983
- 1983-01-20 DD DD83247370A patent/DD233753A3/en unknown
-
1984
- 1984-01-09 SU SU847773265A patent/SU1451165A1/en active
- 1984-01-13 BG BG63870A patent/BG49225A1/en unknown
- 1984-01-17 CS CS84355A patent/CS258414B1/en unknown
- 1984-01-20 JP JP59008376A patent/JPS6062987A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
SU1451165A1 (en) | 1989-01-15 |
DD233753A3 (en) | 1986-03-12 |
BG49225A1 (en) | 1991-09-16 |
JPS6062987A (en) | 1985-04-11 |
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