CS247108B3 - Apparatus for groving of microorganism - Google Patents

Apparatus for groving of microorganism Download PDF

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Publication number
CS247108B3
CS247108B3 CS12582A CS12582A CS247108B3 CS 247108 B3 CS247108 B3 CS 247108B3 CS 12582 A CS12582 A CS 12582A CS 12582 A CS12582 A CS 12582A CS 247108 B3 CS247108 B3 CS 247108B3
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CS
Czechoslovakia
Prior art keywords
nehbí
c6opa
annapat
ajih
cones
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CS12582A
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Czech (cs)
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CS12582A1 (en
Inventor
Alexander J Vinarov
Jaroslav J Skop
Vladimir A Osipov
Valerij A Bykov
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Alexander J Vinarov
Jaroslav J Skop
Vladimir A Osipov
Valerij A Bykov
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Application filed by Alexander J Vinarov, Jaroslav J Skop, Vladimir A Osipov, Valerij A Bykov filed Critical Alexander J Vinarov
Publication of CS12582A1 publication Critical patent/CS12582A1/en
Publication of CS247108B3 publication Critical patent/CS247108B3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/22—Perforated plates, discs or walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Description

11

HsoCpeTeHHe othochtch k MHKpoSHonorHHecKoň npoMbimneHHocTH·HsoCpeTeHHe othochtch k MHKpoSHonorHHecKoň npoMbimneHHocTH ·

B ochobhom HBoOpereHHH no aBTopcxoMy cBHfleTenbCTBy CCCP » 788726 onncaH annapaT ajih BbtpayHBaHHH MHKpoopr3hh3Mob.B ochobhom HBoOpereHHH no aBTopcxoMy cBHfleTenbCTBy CCCP »788726 onncaH annapaT ajih BbtpayHBaHHH MHKpoopr3hh3Mob.

YKasaHHHň annapaT coyepWHT no KpattHeň Mepe ββθ aHanoriwHoň KOHCTpyKUHHYKasaHHH annapaT coyepWHT no KpattHen Mepe ββθ and HanoriwHoo KOHCTpyKUHH

BepTHKanbHue eMKocTH, pasyeneHHbie no BbicoTe na cexyHH πβρφορηροΒβΗΗΜΜΗ ne-peropoAKaMH, b kotopux HaxoflHTCH nnaBaraman HacayKa. BepxHHH ceKyna KaacyoňeMKocTH npeflHaaHaqeHa yna κοηιχθητρηροβηηηη cycneH3HH MHKpooprahhsmob. Annaρβτ CHaOseH πβτργβκβΜΗ yna noysoya ΠΗτβτβπΒΗοίί cpeyu h aspHpywJnero rasa bHKSHHe cexyHH h OTBoya οοΒβτπβΗΗοή KynbTypanbHott jkhakocth h oTpaCoTaHHororasa ms eepxHett ceicyHH. AnnapaT o6opyyoBaH TaK»e ynpKynHynoHHbiMH HacocaMH,ycTaHOBJíeHHWMH Ha jihhhhx nonsopa ňHTaTenbHoft cpeybí b eMKocTH, h ycTpoň-ctbom yjiH c6opa h BMBoya KOHyeHTpHpoBaHHoft ČHOMaccu, πρΗ 3TOM BepxHHe cex-yHy etiKocTH TaHreHUHanbHO noycoeyHHeHM κ ycTpoňcTBy ynn c6opa h BbiBoya kohyeHTpHpoBaHHoň 6noMaccw, a πβτργβκΗ yn« OTBoya oceeTneHHoň KynbTypanbHoňBMAKOCTM H OTBOfla ra3a OflHoft eMKOCTH nOyCOeflHHOHH COOTBeTCTBeHHO κ πηηηηBcacuBaHMH UHpKynHiíHOHHoro Hacoca h naTpyÓKy «jih noyBoya rasa ypyroň βΜκο—CTH. lípu pafioTe annapaTa b nepeyio no xoyy TexHonornnecKoro npoyecca eMKocTbnoyaeTCfl aapHpyioiUHň raa h ocymecTBnnioT BbipanpiBaHHe MHKpoopraHHsMOB b npn-cyTCTBHH nnaBaraneft HacayKH. KoHyeHTpHpoBaHHaa cycneH3HH MHKpoopraHH3MOB H3BepxHeft ceKyHH Kaatyofi eMKOCTH nocTynaeT b ycTpoňCTBo ynn c6opa h BbiBoyaKOHyeHTpaTa 0noMaccbi, a ocseTneHHaa XHflKOCTb noyaeTCH Ha jihhhio BcacbiBaHHnyHpKynayHOHHoro Hacoca Βτοροή θμκοοτη. AspayHH cpeybí βο Βτοροή cmkocth ocyigecTBJíHeTCH nyTeM noyaun b nee rasa, οτβοαημογο hs nepeoň eMKocTH. AHano-γημηο noflKJiroqeHa Tpena h nocneyyKmwe θμκοοτη. OTpaCoTaHHbift ra3 otbohhtchhs nocneflHeň no xoyy TeXHonorEqecKoro npoyecca smkocth η οη μοχθτ 6biTb no-yaH ísfla ouhctkh b CHCTeMy ra3ooňHcTKH.BepTHKanbHue eMKocTH, pasyeneHHbie no BbicoTe on cexyHH πβρφορηροΒβΗΗΜΜΗ ne-peropoAKaMH, b kotopux HaxoflHTCH nnaBaraman HacayKa. BepxHHH ceKyna KaacyoňeMKocTH npeflHaaHaqeHa yna κοηιχθητρηροβηηηη cycloH3HH MHKpooprahhsmob. Ρ β a ρ ρ ρ H H H H H H H H H yn yn yn yn yn yn yn yn yn yn yn yn yn yn yn yn yn yn yn yn yn yn e e e e e e e AnnapaT o6opyyoBaH TaK »e ynpKynHynoHHbiMH HacocaMH, ycTaHOBJíeHHWMH Ha jihhhhx nonsopa ňHTaTenbHoft cpeybí b eMKocTH h ycTpoň-ctbom yjiH c6opa h BMBoya KOHyeHTpHpoBaHHoft tufts, πρΗ 3TOM BepxHHe CEX-YHY etiKocTH TaHreHUHanbHO noycoeyHHeHM κ ycTpoňcTBy ynn c6opa h BbiBoya kohyeHTpHpoBaHHoň 6noMaccw and πβτργβκΗ yn" OTBoya OTHER THREE KynbTypanbHoonBMAKOCTM H OTBOfla ra3a OflHoft eMKOCTH nOyCOeflHHOHH COOTBeTCTBeHHO κ πηηηηBcacuBaHMH UHpKynHiíHOHHoro Hacoca h naTpyÓKy «south noyBoya rasa ypyroň βΜκο — CTH. lipa pafioTe annapaTa b nepeyio no xoyy TexHonornnecKoro npoyecca eMKocTbnoyaeTCfl aapHpyioiUHn raa h ocymecTBnnioT BbipanpiBaHHe MHKpoopraHHsMOB b npn-cyTCTBHH nnaBaraneft HacayKH. KoHyeHTpHpoBaHHaa cycneH3HH MHKpoopraHH3MOB H3BepxHeft ceKyHH Kaatyofi eMKOCTH nocTynaeT b ycTpoňCTBo ynn c6opa h BbiBoyaKOHyeHTpaTa 0noMaccbi and ocseTneHHaa XHflKOCTb noyaeTCH Ha jihhhio BcacbiBaHHnyHpKynayHOHHoro Hacoca Βτοροή θμκοοτη. AspayHH cpeybi οΒ ο ο k k k k k k k k k k k k,,,,,, K K K K K K K K K K K K K K K K AHano-γημηο noflKJiroqeHa Tpena h nocneyyKmwe θμκοοτη. OTpaCoTaHHbift ra3 otbohhtchhs nocneflHen no xoyy TeXHonorEqecKoro npoyecca smkocth η οη μοχθτ 6biTb no-yaH ft ouhctkh b CHCTeMy ra3ooňHcTKH.

HeyocTaTOK ochobhoto annapaTa 3aKn<onaeTCH b tom, hto b cymecTByjomeM ycTpoftcTBe yns cCopa h BMBoya KOHyeHTpHpoBaHHoň 0HOMaccbi npoHcxo«HT HeyocTa-TOHHO 3φφβΚΤΗΒΗΟ ΚΟΗΐχβΗΤΡΗρΟΒβΗΗβ MHKpOOpraHHSMOB B ΠβΗβ.HeyocTaTOK ochobhoto annapaTa 3aKn <onaeTCH b tom, hto b cymecTByjomeM ycTpoftcTBe yns cCopa h BMBoya KOHyeHTpHpoBaHHoon 0HOMaccbi npoHcxo «HT HeyocTa-TOHHO 3φφβΚΤΗΒΗΟ ΚΟΗΐχβΗΤΡΗρΟΒβΗΗβ MHKpOOpraHHSMOB B ΠβΗβ.

Uenbio yonojiHHTenbHoro HSoCpeTeHHH HBjíHeTCH noBbímeHHe CTeneHH KOHyeHTpH-ΡΟΒβηηη dHOMaccM. yKasaHHan yenb yocTHraeTCH τβΜ, hto b annapaTe pjw Bbipa-πκηβηηηη MHKpoopraHHSMOB no aBTopcKOMy CBHfleTenbCTBy CCCP N“ 788726 ycTpoň- 247108 -2- ctbo fljia c6opa h BblBOfla XOHUeHTpHpOBaHHOH 6noMaccbi cHaSaceHo xaMepoň ocyiu-kh neHbí, o6pa3OBaHHoň UHJiHHflpHnecxoň oSenaňxoň h npHxpenneHHbíMH κ Heň 6onbUIHMH OCHOBaHHHMH flByMH yceneHHblMH KOHycaMH, OÓpameHHblMH OCHOBaHHHMHBBepX,h pacnoJiojKeHHbíMH ouhh Hap; ,n,pyrnM c o6pa3OBaHneM Meacfly hhmh 3a3opa ajih npo-xofla ocymeHHoň neHbí κ neHoracHTenio, npn stom yron HaxnoHa o6pa3yromeň Bepx-Hero KoHyca cocTaBnneT 45-60°, a ηηχηθγο 20-45°.Uenbio yonojiHHTenbHoro HSoCpeTeHHH HBjiHeTCH noBbHHe CTeneHH KOHyeHTpH-ΡΟΒβηηη dHOMaccM. yKasaHHan Yenbo yocTHraeTCH τβΜ, HTO b annapaTe pjw Bbipa-πκηβηηηη MHKpoopraHHSMOB no aBTopcKOMy CBHfleTenbCTBy CCCP N "788726 247108 ycTpoň- -2- ctbo fljia c6opa h BblBOfla XOHUeHTpHpOBaHHOH 6noMaccbi cHaSaceHo xaMepoň ocyiu-kh neHbí, o6pa3OBaHHoň UHJiHHflpHnecxoň oSenaňxoň h npHxpenneHHbíMH κ Hen 6onbUIHMH OCHOBaHHHMH flByMH yceneHHblMH KOHycaMH, OopameHHblMH OCHOBHHHMHBBepX, h pacnoJiojKeHHbMH ouhh Hap; N, c pyrnM o6pa3OBaHneM Meacfly hhmh 3a3opa ajih NPO-xofla ocymeHHoň neHbí κ neHoracHTenio, one hundred meters npn yron HaxnoHa o6pa3yromeň Bepx-Hero KoHyca cocTaBnneT 45-60 ° and 20-45 ° ηηχηθγο.

Ha Φηγ . 1 - o6iuhh βηλ annapaTa απη BbipamHBaHHH MHxpoopraHH3MOB, Φηγ.2-BHfl CBepxy, Ha Φηγ.3 - pa3pe3 B-B no ycTpoňcTBy hjih c6opa h BblBOfla koh-ΙξθΗΤρΗρΟΒ aHHOÍi .ÓHOMaCCbl .Ha Φηγ. BbipamHBaHHH MHxpoopraHH3MOB, Φηγ.2-BHfl CBepxy, Ha Φηγ.3 - pa3pe3 B-B no ycT leastcTBy hjih c6opa h BblBOfla koh-ΙξθΗΤρΗρΟΒ aHHOi .ÓHOMaCCbl.

AnnapaT cofleputHT no xpaňHeň Mepe flBe aHanori«Hbie no xoHCTpyxflHH Βερτκ-KanbHbie KonoHHbie θμχοοτη 1. pa3fleneHHbie no.BbicoTe Ha cexflHH πθρφορκροΒθΗ-HbiMH neperopoflxaMH 2, b χοτορωχ HaxoflHTCH nnaBaioiuaH Hacauxa (Ha Φηγ.1 HeH3o6paaceHa) . BepxHHH cexuHH 3 xaacfloň θμκοοτη npeflHa3HaneHa ajih χοηαθητρη-pOBaHHH CyeneH3HH MHKpOOpraHH3MOB.AnnapaT cofleputHT no xpaňHeň Mepe flBe aHanori «Hbie no xoHCTpyxflHH Βερτκ-KanbHbie KonoHHbie θμχοοτη first pa3fleneHHbie no.BbicoTe Ha cexflHH πθρφορκροΒθΗ-HbiMH neperopoflxaMH 2 b χοτορωχ HaxoflHTCH nnaBaioiuaH Hacauxa (IIa Φηγ.1 HeH3o6paaceHa). BepxHHH cexuHH 3 xaacfloň θμκοοτη npeflHa3HaneHa ajih χοηαθητρη-pOBaHHH CyeneH3HH MHKpOOpraHH3MOB.

Emkocth 1 HMeioT naTpyfiKH 4 flnn noflBOfla nHTaTenbHoň cpeflbí, naTpy6xH 5fljiH noflBOfla aspHpyeMoro ra3a κ ηηικηθη cexuHH, a Taxise naTpyóxň 6 ajih otbo-p;a ocBeTJieHHOíi xynbTypanbHoň ikhaxocth h naTpyóxH 7 huh OTBOfla OTpaOoTaH-ηογο ra3a H3 BepxHeň cexuHH.Emkocth 1 HMeioT naTpyfiKH 4 flnn noflBOfla nHTaTenbHoň cpeflbí, naTpy6xH 5fljiH noflBOfla aspHpyeMoro ra3a κ ηηικηθη cexuHH and Taxis naTpyóxň ajih otbo 6-p; and ocBeTJieHHOíi xynbTypanbHoň ikhaxocth h naTpyóxH 7-huh OTBOfla OTpaOoTaH ηογο ra3a H3 BepxHeň cexuHH.

BepxHHe cexuHH 3 eMxocTeň 1 TaHreHUHanbHo noflcoeflHHeHbí κ ycTpoňcTBy 8flUH c6opa H BblBOfla XOHUeHTpHpOBaHHOH ÓHOMaCCbl, ΠρΗΗβΜ ΟβίζΗή BblBOfl 6HOMaCCbIH3 annapaTa ocymecTBnneTCH b HanpaBneHHH CTpenxň A.BepxHHe cexuHH 3 eMxocTen 1 TaHreHUHanbHo noflcoeflHHeHbbKkcT at leastTBy 8fUH c6opa H BblBOfla XOHUeHTpHpOBaHHOH ÓHOMaCCbl, ΠρΗΗβΜ blβίζΗή BblBOfl 6HOMaCCbIH3 annapaTa ocymecTBnneTCH b HanpaBneHHH CTpenxen A.

AnnapaT cHa6nceH uHpxynnuHOHHbíMH HacocaMH 9. Πρκ stom naTpyóox 6 auh ot-BOfla ocBeTJieHHoň xynbTypanbHoň ikhaxocth h3 nepBoň no xofly TexHonornqecxo-ro npouecca eMKoera noflcoeflHHeH κ BcacbiBatomeMy naTpySxy Hacoca 9 BTopoňeMKocTH, a naTpy6ox 6 BTopoň θμχοοτη aHanorHHHMM o6pa3OM noflXjHoqeH κ Bca-cbieaioineMy naTpyfixy UHpxynHUHpHHoro Hacoca 9 TpeTbeň eMxocTH h Tax uanee. H3 nocneflHeů no xouy TexHonorHnecxoro npouecca eMxocTH xynbTypanbHyio KHfl-xocTb oTBOflHT no naTpyOxy 6, Ha Φηγ. He noxa3aHHOMy. IIaTpy6xH 7 flnn OTBOfla oTpaSoTaHHoro ra3a H3 xaacfloň xonoHHoň θμχοοτη co-eflHHeHbí c naTpySxaMH 5 unn noflBOfla aapHpyiomero ra3a b nocnenyiomyto no xopynpouecca eMxocTb 1. H3 naTpyÓxa 7 nocneflneň no xouy TexHonorHuecxoro npo-uecca eMxocTH npoH3BoflHTCH noflawa oTpa6oTaHHoro ra3a no CTpenxe C na eroOHHCTXy. YCTpOňCTBO 8 (φΗΓ.3) flUH CČOpa H BblBOfla XOHUeHTpHpOBaHHOň 6HOMaCCbI co—,uepjKHT cSophhx xoHueHTpaTa 10, b BepxHeň uacTH χοτοροΓο pa3MemeHbi neHora-CHTeub 11 h pacnoJionceHHyio CHapyxtH Hero coocho xaMepy 12 ocynixn neHbí, 06-paaoBaHHyio unnHHflpHHecxoň o6enaňxoň 13 h npHxpenueHHbíMH κ Heň OojibniHMHOCHOBaHHHMH flByMH yCeneHHblMH XOHyeaMH 14 H 15, OÓpameHHblMH MeHblIIHMH ocho-B3HHHMH BBepX H p 3CnQJI01KeHHbIMH OflHH Hafl flpyrHM C 3a3OpOM MeiKfly HHMH flJIHnpoxofla ocyuieHHoň neHbí x neHoracHTenio 11, npu stom yron HaxnoHa o6pa3yio-meň BepxHero xoHyca cocTaBnneT 45-60°, a HH»Hero 20-45°.AnnapaT cHa6nceH uHpxynnuHOHHbíMH HacocaMH ninth Πρκ one hundred meters naTpyóox AUH 6-ol BOfla ocBeTJieHHoň xynbTypanbHoň ikhaxocth h3 nepBoň no xofly TexHonornqecxo-ro npouecca eMKoera noflcoeflHHeH κ BcacbiBatomeMy naTpySxy Hacoca BTopoňeMKocTH 9, and 6 naTpy6ox BTopoň θμχοοτη aHanorHHHMM o6pa3OM noflXjHoqeH κ BCA cbieaioineMy naTpyfixy UHpxynHUHpHHoro Hacoca 9 TpeTbe eMxocTH h Tax uanee. H3 nocneflHeu no xouy TexHonorHnecxoro npouecca eMxocTH xynbTypanbHyio KHfl-xocTb oTBOflHT no naTpyOxy 6, Ha Φηγ. He noxa3aHHOMy. IIaTpy6xH 7 flnn OTBOfla oTpaSoTaHHoro ra3a H3 xaacfloň xonoHHoň θμχοοτη co eflHHeHbí c naTpySxaMH 5 unn noflBOfla aapHpyiomero ra3a b nocnenyiomyto well xopynpouecca eMxocTb H3 naTpyÓxa first seven amino nocneflneň xouy TexHonorHuecxoro NPO-UECC eMxocTH npoH3BoflHTCH noflawa oTpa6oTaHHoro ra3a well CTpenxe eroOHHCTXy C. YCTpOňCTBO 8 (φΗΓ.3) FluH CČOpa H BblBOfla XOHUeHTpHpOBaHHOň 6HOMaCCbI CO- uepjKHT cSophhx xoHueHTpaTa 10 b BepxHeň uacTH χοτοροΓο pa3MemeHbi burn-CHTeub 11 h pacnoJionceHHyio CHapyxtH Hero COOCH xaMepy ocynixn neHbí 12, 06-paaoBaHHyio unnHHflpHHecxoň o6enaňxoň 13h npHxpenueHHbíMH κ Hen OojibniHMHOCHOBaHHHMH flByMH yCeneHHblMH XOHyeaMH 14 H 15 OÓpameHHblMH MeHblIIHMH ocho-B3HHHMH BBepX H p 3CnQJI01KeHHbIMH OflHH Hyflo flpyrHM C 3a3OpOM MeiKfly HHMH flJIHnpoxofla ocyuieHHoň neHbí x 11 neHoracHTenio NPU one hundred meters yron HaxnoHa o6pa3yio Men BepxHero xoHyca cocTaBnneT 45-60 °, and HH »Hero 20-45 °.

BepxHHe cexflHH 3 xojiohhm 1 noflcoeflHHeHbí x xaMepe 12 ocymxn neHbí.BepxHHe cexflHH 3 xiohohm 1 noflcoeflHHeHbb x xaMepe 12 ocymxn noth.

AnnapaT paóoTaeT cneflyronuiM o6pa3OM. B nepByio no xouy TexHonorHuecxoro npouecca eMxocTb 1 nepe3 naTpySox 5noflaioT aapHpyromHň ra3, HanpHMep BO3flyx, h ocymecTBUHiOT BbipaiUHBaHHe mhxpo-opraHH3MOB b npHcyTCTBHH nuaBaioiueň HacaflXH, pa3MemeHHoň b cexuHHx, o6pa3o-BaHHbix πθρφορΗροΒΗΗΗΜΜΗ neperopoflxaMH 2. Iloflany nHTaTenbHoň cpeflbí h yrne-poflcoflepacamero cy6cTpaTa npoH3BOflHT nepe3 naTpyČxn 4. KoHueHTpupoBaHHaHcycneH3HH MnxpoopraHH3MOB H3 BepxHeň cexuHH 3 noflaeTca b xaMepy 12 ocymxHneHbí, b χοτοροή npoHcxoflHT uaJibHeňmee xoHueHTpHpoBaHne MHXpoopraHH3MOB,BCJieflCTBHe ocynixn neHbí h ee rameHHH b neHoracHTene 11 (ecnn TpeGyeTcn) .KoHueHTpaT οτβοαητ h3 cfíopHHxa 10 no CTpenxe A. OcBeTneHHyio xynbTypanbHyio 247108 -3- jKHQKocTb uepea naTpy6oK 6 noflaiOT κ BcacbtBatometoy naTpy6Ky flHpKynHflHOHHoroHacoca 9 βτοροϋ eMKocTH 1. K 3TOMy »e naTpy6Ky flHpKynHflHOHHoro Hacoca flo-nonHHTenbHo noflaiOT nHTaTeflbHyw cpefly h yrnepoflcoflepacaniHň cyČCTpaT. flaneecMecb HacocoM 9 noflaeTCH b cooTBeTCTByiomHe naTpyčKH Βτοροϋ κοποηηοΛ θμ-kocth.AnnapaT paoTaeT cneflyronuiM o6pa3OM. B nepByio no xouy TexHonorHuecxoro npouecca eMxocTb 1 nepe3 naTpySox 5noflaioT aapHpyromHň RA3, HanpHMep BO3flyx h ocymecTBUHiOT BbipaiUHBaHHe mhxpo opraHH3MOB-b npHcyTCTBHH nuaBaioiueň HacaflXH, pa3MemeHHoň b cexuHHx, o6pa3o-BaHHbix πθρφορΗροΒΗΗΗΜΜΗ neperopoflxaMH second Iloflany nHTaTenbHoň cpeflbí h yrne-poflcoflepacamero cy6cTpaTa npoH3BOflHT nepe3 naTpyČxn fourth KoHueHTpupoBaHHaHcycneH3HH MnxpoopraHH3MOB H3 BepxHeň cexuHH 3 noflaeTca b xaMepy 12 ocymxHneHbí b χοτοροή npoHcxoflHT uaJibHeňmee xoHueHTpHpoBaHne MHXpoopraHH3MOB, BCJieflCTBHe ocynixn neHbí h ee rameHHH b neHoracHTene 11 (ecnn TpeGyeTcn) .KoHueHTpaT οτβοαητ h3 cfíopHHxa 10 A. well CTpenxe OcBeTneHHyio xynbTypanbHyio 247,108 -3- K ac K K ep n K o 6 K K K Bc K K K na K K n n n n n n n n 3 n n n n n n n ac ac ac. flaneecMecb HacocoM 9 noflaeTCH b cooTBeTCTByiomHe naTpychKH Βτοροϋ κοποηηοΛ θμ-kocth.

AspaflHH cpepja bo BTopoň θμκοοτη ocymeCTBnHeTCH 3a cneT noflanH b naTpy-6ok 5 rasa, οτβοαημογο H3 nepBoň βΜκοοτκ nepee naTpy6oK 7.AspaflHH cpepja bo BTopoň ocymeCTBnHeTCH 3a cneT noflanH b naTpy-6ok 5 rasa, οτβοαημογο H3 nepBoň βΜκοοτκ nepee naTpy6oK 7.

Bo Βτοροή h nocjienyromnx βΜκοοτΛχ ocymecTBnaeTca ananormnuit npofleccpenpepblBHOrO BbipaiUHBaHHH MHKpOOpraHH3MOB . H3 nocneflHeň no xofly npouecca cmkocth οτβΗρβιοτ nepe3 naTpyĎoK 6 oceeT-neHHyio KyjibTypaJibHy» khakoctb, a qepea naTpySoK 7 buboaht OTpa6oTaHHbiňras no οτρβηκβ C, κοτορωή Μο»βτ 6ωτβ noflaH hs onncTKy. B ycTpoňCTBe ajxh c6opa κ BbtBOfla KOHfleHTpHpoBaHHoň ČHOMaccbi npoHcxoflHTnoBbtmeHHe ετβπβΗΗ KOHfleHTpaflHH MHKpoopraHH3MOB b neHHoň $a3e, nocTynatomeňH3 BepxHnx ceKflnň 3 kojxohh 1. íleHa nocTynaeT b KaMepy 12 ocyniKH πθημ, rneBCJieflCTBHe TypĎyjieHTHoro nepeMemeHHH neHbt BBepx no HanpaBjíeHMo κ c6opHnxy10, npoHcxoflHT HHTeHCHBHaa ee ocymxa. B cnytae HeoĎxoflHMOCTH oKoHnaTenb-Hoe raute Hne neHbí Μο«βτ npoHSBOflHTbcn nenoracHTeneM 11. KoHfleHTpaT 6nÓMac-cu co6HpaeTca b. c6opHHKe 10 h otboahtch nepe3 nrrynep no cTpejixe A.Bo noc ο ρ noc noc noc noc noc noc noc noc ym ym ym ym ym ym ym ym ym ym ym ym ym ym ym b MH MH b b BbipaiUHBaHHH MHKpOOpraHH3MOB. H3 nocneflHen no xofly npouecca cmkocth οτβΗρβιοτ nepe3 naTpyĎoK 6 ocEt-neHHyio KyjibTypaJibHy »khakoctb, and qepea naTpySoK 7 buboaht OTpa6oTaHHbiňras no οτρβηκβ C, κοτορωή Μο» βτ 6ωτβ noflaH hs onncTKy. B ycTpoňCTBe ajxh c6opa κ BbtBOfla KOHfleHTpHpoBaHHoň ČHOMaccbi npoHcxoflHTnoBbtmeHHe ετβπβΗΗ KOHfleHTpaflHH MHKpoopraHH3MOB b neHHoň $ A3E, nocTynatomeňH3 BepxHnx ceKflnň kojxohh 3 positioned adjacent the first nocTynaeT b KaMepy 12 ocyniKH πθημ, rneBCJieflCTBHe TypĎyjieHTHoro nepeMemeHHH neHbt BBepx well HanpaBjíeHMo κ c6opHnxy10, npoHcxoflHT HHTeHCHBHaa ocymxa ee. B cnytae HeoĎxoflHMOCTH oKoHnaTenb-Hoe rne NeHbí Μο «βτ npoHSBOflHTbcn not hhhhhhhhhhhhhhhhQeh 10h otboahtch nepe3 nrrynep no cTpejixe A.

KoHCTpyKTHBHoe βηποπηθηηθ KaMepw ocyniKH neHbí cnocoČCTByeT ycKopeHmonpoTexaHHfl np ope cca o6e3BO»HBaHHH neHbí. JfeiflKaa $aaa no flHumy cTexaeT bBepxHioio ceKflroo 3 kojiohhw 1 h b BHfle ocBeTneflHoň jkhakocth nepe3 naTpyČKH 6nocTynaer κ flHpKynsflHOHHbíM HacocaM 9.KoHCTpyKTHBHoe βηποπηθηηθ KaMepw ocyniKH neHbí cnocoČCTByeT ycKopeHmonpoTexaHHfl np ope cca o6e3BO »HBaHHH neHbí. JfeiflKaa $ aaa no flHumy cTexaeT bBepxHioio ceKflroo 3 kojiohhw 1 h b BHfle ocBeTneflHoň jkhakocth nepe3 naTpyČKH 6nocTynaer κ flHpKynsflHOHHbM HacocaM 9.

BbinonHeHHe xaMepbi ocyniKH neHbí b BHfle flHnHHflpHwecKoň oÓeňaňKH η πρκκρβπ-neHHbix κ Heň 6οπβπιημη ocHOBaHHHMH AByx yceneHHbix KOHycoB, oSpameHHbix MeHb-ιπημη ocHOBaHHHMH BBepx h pacnonoxceHHbix oflHH Hafl flpyrroťc o6pa3OBaHHeM Meac-fly hhmh 3a3opa ajih npoxofla ocymeHHoň neHbt κ neHoracHTenw 6naroflap« BbiGopyHaxnoHa oSpasyromeň eepxHero KOHyca 45-60°, a HHJKHero 20-45°, cnocoScTByeTHanpaBneHHOMy RBHxeHHto neHbí b saKpyneHHOM (TaHřeHfluaJibHbtň bbor) · no bocxo-flHttieň cnHpanH ποτοκ c H3MeHHtoni,HMCH flaBJíeHneM Ha neHy 3a cneT nepeMeHHoroceqeHHH xaMepw, BCjieflCTBHe nero floeruraeTCH yBennweHHe ετβπβΗΗ KOHfleHT-PHpoBaHHH SHOMaCCht. ’ PasMetneHHbBÍ BHyTpH KaMepbi ocyntKu coocho eň c6ophhk KOHfleHTpaTa c .ycTa-HOBJíeHHbíM b BepxHeň ero hhcth neHoracHTejieM oSecnenHBaeT 3φφεκτΗΒΗοβ ocy-menne neHbí, nocne ee KOHfleHTPHpoBaHHH b xaMepe ocyntKH h pa3fleneHHe cyc-neH3HH na rasoeyio 4>a3y h KOHfleHTpupoBaHHyto SHOMaccy, b pe3yjihTaTe nero HaBbixofle ycTpoňcTBa flocTHraeTca KOHfleHTpaflHH SHOMaccw 60-80 r/n.BbinonHeHHe xaMepbi ocyniKH neHbí b BHfle flHnHHflpHwecKoň oÓeňaňKH η πρκκρβπ-neHHbix κ Hen 6οπβπιημη ocHOBaHHHMH AByx yceneHHbix KOHycoB, oSpameHHbix MeHb-ιπημη ocHOBaHHHMH BBepx h pacnonoxceHHbix oflHH Hyflo flpyrroťc o6pa3OBaHHeM MEAC-fly hhmh 3a3opa ajih npoxofla ocymeHHoň neHbt κ neHoracHTenw 6naroflap «BbiGopyHaxnoHa oSpasyromeň eepxHero KOHyca 45 -60 °, and HHJKHero 20-45 ° cnocoScTByeTHanpaBneHHOMy RBHxeHHto neHbí b saKpyneHHOM (TaHřeHfluaJibHbtň bbor) · nO-BocX flHttieň cnHpanH ποτοκ c H3MeHHtoni, HMCH flaBJíeHneM Ha tenderness 3a CNet nepeMeHHoroceqeHHH xaMepw, BCjieflCTBHe Nero floeruraeTCH yBennweHHe ετβπβΗΗ KOHfleHT-PHpoBaHHH SHOMaCCht. 'PasMetneHHbBÍ BHyTpH KaMepbi ocyntKu COOCH en c6ophhk KOHfleHTpaTa .ycTa c-b HOBJíeHHbíM BepxHeň ero hhcth neHoracHTejieM oSecnenHBaeT 3φφεκτΗΒΗοβ ocy neHbí-exchange, night ee KOHfleHTPHpoBaHHH xaMepe ocyntKH b h c? Pa3fleneHHe neH3HH to rasoeyio 4> h a3y KOHfleHTpupoBaHHyto SHOMaccy b pe3yjihTaTe nero HaBbixofle ycT leastcBB flocTHraeTca KOHfleHTpafHH SHOMaccw 60-80 r / n.

Hcnonb3OBaHne ycTpoňcTBa ajih cSopa h OTBOfla κοηαθητρηροββηηοη SnoMaccwΠΟ3Β0ΗΗβΤ nOBblCHTb CTeneHb KOHfleHTPHpoBaHHH MHKpOOpraHH3MOB B' ΠβΗΘ H OT“BOflHTb H3 annapaTa ČHOMaccy c Sonbtneň KOHfleHTpaflHeň μηκροοργηηηβμοβ, htocoKpatqaeT 3aTpaTbt Ha CTaflHH BbtfleneHHH μηκροοργβηη3Μοβ·Hcnonb3OBaHne ycTpoňcTBa ajih ČSOP h OTBOfla κοηαθητρηροββηηοη SnoMaccwΠΟ3Β0ΗΗβΤ nOBblCHTb CTeneHb KOHfleHTPHpoBaHHH MHKpOOpraHH3MOB B 'ΠβΗΘ H OT "BOflHTb H3 annapaTa tufts c Sonbtneň KOHfleHTpaflHeň μηκροοργηηηβμοβ, htocoKpatqaeT 3aTpaTbt Ha CTaflHH BbtfleneHHH μηκροοργβηη3Μοβ ·

Hrace .npHBefleHbí cpaBHHTenbHbte flaHHbte no KOHfleHTpaflHH flpoarateBbtx Μκκροορ-γηηη3μοβ b neHe, nojiyneHHbte nph HcnbiTaHHH MOfleneň ycTpoftcTB, βμποπηθηημχno HSBecTHoMy h npeflnaraeMOMy TexHHnecKHM pemeHHHM: 247108 4 c npHMeHeHHeM H3- no npěflnarae- BecTHoro ycTpoň- CTBa MOMy ycTpoň-CTBy 1 2 3 HanajibHaH KOHfleHTpaflHHČHOMaCCbl 20,5 r/n 20,0 r/n BpeMH KOHfleHTpHpOBaHHH 15 MHH 15 MHH KoHenHan KOHfleHTpaflHHÓHOMaCCbl 45 r/n 70 r/n ΦΟΡΜΥΠΑ Η30ΕΡΕΤΕΗΗΗ 1. AnnapaT απη BbipantHBaHHH MHKpoopraHH3MoB no ašTopcKOMy CBHfleTenbCTByCCCP 8* 788726, o tji h >taio!HHftce τθμ, hto, c flenb» ποβμβιθηηη CTene-HH KOHfleHTpHpOBaHHH ÓHOMaCCbl, yCTpOHCTBO RSIX c6opa H BMBOfla KOHfleHTPHpO“BaHHoň 6noMaccbi cHa6»eHo KaMepoň ocyniKH neHbí, o6pa3OBaHHoň iíHJiHHflpunecKoňo6enaňKoň η npHKpenneHHbíMH κ Heň 6οπβπιημη οοηοβηηηημη flByMH yceneHHbíMH ko-HycaMH, oSpameHHblMH MeHbmHMH OCHOBaHHHMH BBepX H pacnOJIOSCeHHbJMn OflHH HaflflpyruM c 3a3opoM Meatfly hhmh aah npoxofla ocymeHHoň nem κ neHoracirrento,npH 3T0M yron HaxjioHa o6pa3yromeň BepxHero KOHyca cocTaBnaeT 45-60°, aHHXHero 20-45°.Players .npHBefleHbí cpaBHHTenbHbte flaHHbte well KOHfleHTpaflHH flpoarateBbtx Μκκροορ-b γηηη3μοβ Nehe, nojiyneHHbte NPH HcnbiTaHHH MOfleneň ycTpoftcTB, βμποπηθηημχno HSBecTHoMy h npeflnaraeMOMy TexHHnecKHM pemeHHHM: 4 247 108 C H 3 amino npHMeHeHHeM npěflnarae- BecTHoro ycTpoň- CTAB MOMy ycTpoň-CTBy 1 2 3 HanajibHaH KOHfleHTpaflHHČHOMaCCbl 20 , 5 r / n 20.0 r / n BpeMH KOHfleHTpHpOBaHHH 15 MHH 15 MHH KoHenHan KOHfleHTpaflHHOHHomaCCbl 45 r / n 70 r / n ΑΠΑ Η30ΕΡΕΤΕΗΗΗ 1. AnnapaT απη BbipantHBaHHH MHKpoopraHH3MoB , HTO c flenb »ποβμβιθηηη reading-HH KOHfleHTpHpOBaHHH ÓHOMaCCbl, yCTpOHCTBO RSIX c6opa H BMBOfla KOHfleHTPHpO" BaHHoň 6noMaccbi cHa6 »EHO KaMepoň ocyniKH neHbí, o6pa3OBaHHoň iíHJiHHflpunecKoňo6enaňKoň npHKpenneHHbíMH η κ Hen 6οπβπιημη οοηοβηηηημη flByMH yceneHHbíMH co-HycaMH, oSpameHHblMH MeHbmHMH OCHOBaHHHMH BBepX H pacnOJIOSC eHHbJMn OflHH HaflflpyruM c 3a3opoM Meatfly hhhh ah npoxofla ocymeHHoNHoracirrento, npH 3T0M yron HaxjioHa o6pa3yromene BepxHero KOHyca cocTaBnaeT 45-60 °, aHXHero 20-45 °.

AHHOTAUHSAHHOTAUHS

AnnapaT ajih BbipamHBaHHH MHKpoopraHH3MOB npeflHa3HaneH ajih Hčnojib3OBaHHHb MHKpoÓHonornneČKoň npoMbimneHHocTH npn BefleHnn npoflecca φερΜβΗΤ3ΑΗΗ HayrnepoflcoflepacameM cySČTpaTe.AnnapaT ajih BbipamHBaHHH MHKpoopraHH3MOB npeflHa3HaneH ajih Hčnojib3OBaHHHb MHKpoÓHonornneČKoň npoMbimneHHocTH npn BefleHnn npoflecca φερΜβΗΤ3ΑΗΗ HayrnepoflcoflepacameM cySČTpaTe.

Uejib Η3θ6ρβτεΗΗΗ - noBbímeHHe cTeneHH KOHfleHTpHpOBaHHH MHKpoopraHH3MOBB Π6Η6. YKa3aHHaH flenb flocTHraeTcn τθμ, ητο b annapaTe απη BbipamHBaHHH Μηκρο-opraHH3MOB no aBTopcKOMy CBHfleTenbCTBy CCCP Ν’ 788726 ycTpoňCTBO flna c6opah Bbipofla KoHfleHTpHpoBaHHoíí SnoMaccbi coflepxHT xaMepy ocymKH neHbí, o6pa3o-BaHHyio flHJiHHflpHnecKoň oSenaňxoň h npuKpenneHHbíMH κ Heň 6οπβιπημη ocHOBaHH-HMH yceneHHbíMH KOHycaMH, pacnonoaceHHbíMH orhh Hafl flpyrHM c 3a3opoM Meacflyhhmh flJiH npoxofla ocymeHHoň neHbí κ neHoracHTen». Yron HaxnoHa o6paay»meňBepxHero xoHyca cocTaBnneT 45-60°, a ηηκηθγο. - 20-45°.Uejib θ3θ6ρβτεΗΗΗ - noBbímeHHe cTeneHH KOHfleHTpHpOBaHHH MHKpoopraHH3MOBB Π6Η6. YKa3aHHaH flenb flocTHraeTcn τθμ, ητο b annapaTe απη BbipamHBaHHH Μηκρο-opraHH3MOB no aBTopcKOMy CBHfleTenbCTBy CCCP Ν '788726 ycTpoňCTBO flna c6opah Bbipofla KoHfleHTpHpoBaHHoíí SnoMaccbi coflepxHT xaMepy ocymKH neHbí, o6pa3o-BaHHyio flHJiHHflpHnecKoň oSenaňxoň h npuKpenneHHbíMH κ Hen 6οπβιπημη ocHOBaHH-HMH yceneHHbíMH KOHycaMH, pacnonoaceHHbíMH orhh Hyflo flpyrHM c 3a3opoM Meacflyhhmh flJiH npoxofla ocymeHHoo neHbí κ neHoracHTen ». Yron HaxnoHa o6paay »meBepxHero xoHyca cocTaBnneT 45-60 °, and ηηκηθγο. - 20-45 °.

IlpH3HaHo Η3θ6ρβτβΗΗβΜ no pe3ynbTaTaM 3KcnepTH3bi, ocyniecTBJíeHHoň Tocyflap-CTBeHHblM ΚοΜΗΤβΤΡΜ CCCP ΠΟ flejiaM HSOÓpeTeHHřl H OTKpbITHň. 2 qepTeaca 247108IlpH3HaHo Η3θ6ρβτβΗΗβΜ no pe3ynbTaTaM 3KcnepTH3bi, ocyniecTBJíeHHoň Tocyflap-CTBeHHblM ΚοΜΗΤβΤΡΜ CCCP ΠΟ flejiaM HSOÓpeTeHHøl H OTKpbITHn. 2 qepTeaca 247108

Claims (1)

247 108 PfiEDMfiT VYNÁLEZU 1· Zařízení na pěstování mikroorganismů podle autorskéhoosvědčení č. 259 31θ» vyznačující se tím, že za účelemzvýšení stupně koncentrace biomasy je zařízení na shroma-žčování a vývod koncentrované biomasy doplněno komorou navysoušení pěny, kterou tvoří válcovitý plášř s dvěma komo-lými kužely, které jsou k plášti připevněny velkými základ-nami a menšími základnami jsou obráceny směrem vzhůru, při-čemž kužely jsou umístěny nad sebou a mezi nimi je mezerana průohod vysušené pěny k odpěňovadlu s tím, že úhel sklo-nu tvořící přímky horního kužele je 45 až 60 0 a spodníhokužele 20 až 45 °·BACKGROUND OF THE INVENTION 1. The microorganism cultivation device of U.S. Pat. No. 259,331, characterized in that, in order to increase the level of biomass concentration, the concentrated biomass collection and discharge device is supplemented by a foam drying chamber formed by a cylindrical shell with two compartments. The tapered cones, which are fastened to the shell with large bases and smaller bases, face upwards, the cones being placed one above the other and between them a gap between the dried foam and the defoamer, with the angle of glass forming the lines of the upper cone. 45 to 60 0 and 20 to 45 ° lower
CS12582A 1981-04-03 1982-01-06 Apparatus for groving of microorganism CS247108B3 (en)

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EP0339356A1 (en) * 1988-04-20 1989-11-02 Fried. Krupp AG Hoesch-Krupp Process and device for the production of baker's yeast
EP0616759B1 (en) * 1993-03-22 1997-05-28 Otmar Gojcic Process and apparatus to produce concentrated protein extract from liquid phase of manure

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US1732921A (en) * 1926-03-06 1929-10-22 Anheuser Busch Process for aerobic fermentation
DK139742B (en) * 1975-06-18 1979-04-09 Niro Atomizer As Centrifugal separator for separating liquid and gas.
AT336517B (en) * 1975-07-07 1977-05-10 Waagner Biro Ag METHOD AND EQUIPMENT FOR TREATMENT OF LIQUIDS, SUCH AS E. TRUBEN WITH A FLUID
SU653295A1 (en) * 1977-04-15 1979-03-25 Предприятие П/Я Р-6729 Microorganism growing apparatus
SU708696A1 (en) * 1978-03-31 1986-11-15 Всесоюзный научно-исследовательский институт биосинтеза белковых веществ Apparatus for growing microorganisms

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CS12582A1 (en) 1985-06-13
BG40749A1 (en) 1987-02-16
DD245568A3 (en) 1987-05-13
FR2503181A1 (en) 1982-10-08
FR2503181B1 (en) 1984-05-04

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