CS245474B1 - A method of preparing a plant tissue culture sample for cytometric analysis - Google Patents

Abstract

é tissue for cytometric analysis. In the method of preparing a plant tissue culture sample for cytometric analysis, including processing the sample with an extraction solution followed by staining the extract with methylene blue, ee uses an extraction solution with the following composition: % by weight 1.95 to 2.05 2.45 to 2.55 4.95 to 5.05 remainder, sample extraction calcium chloride pectinase cellocandin distilled water, wherein the treatment with the solution takes place at a temperature of 26 i 1 C for 4 to 5 hours and the volumes of the sample and extraction solution are 1:1.

Description

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Claims (2)

245474 14 SUBJECT OF EXCHANGE OF SZU A method of preparing a plant tissue culture sample for cytometric analysis by treating a sample with an extracting solution followed by coloring the extract with ethylene blue, wherein a leaching solution of the following composition is used to prevent cell damage and increase the degree of survival of the sample: % by weight of calcium chloridePactinacalocandeddistilled water 1.95 to 2.052.45 to 2.55 4.95 to 5.05%, the sample being treated with leaching solutions at 26 ° C for 4 to 5 hours, the volume of sample to leach solution is 1: 1. Recognized by the invention on the basis of the results of expertise, conducted by the State Committee for Inventions and Discoveries SSSH, Moscow, SU.