CS244335B1 - Fodder mixtures' admixture for feeding of domestic animals - Google Patents
Fodder mixtures' admixture for feeding of domestic animals Download PDFInfo
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- CS244335B1 CS244335B1 CS848916A CS891684A CS244335B1 CS 244335 B1 CS244335 B1 CS 244335B1 CS 848916 A CS848916 A CS 848916A CS 891684 A CS891684 A CS 891684A CS 244335 B1 CS244335 B1 CS 244335B1
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- Czechoslovakia
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- feed
- admixture
- feeding
- carbohydrates
- domestic animals
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- 241001465754 Metazoa Species 0.000 title description 3
- 239000000203 mixture Substances 0.000 title description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims abstract description 9
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 9
- 235000014633 carbohydrates Nutrition 0.000 claims abstract description 9
- 150000003839 salts Chemical class 0.000 claims abstract description 9
- 235000018102 proteins Nutrition 0.000 claims abstract description 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 8
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 8
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims abstract description 7
- 150000002632 lipids Chemical class 0.000 claims abstract description 7
- 230000000813 microbial effect Effects 0.000 claims abstract description 6
- 239000000126 substance Substances 0.000 claims abstract description 6
- 235000020357 syrup Nutrition 0.000 claims abstract description 5
- 239000006188 syrup Substances 0.000 claims abstract description 5
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 4
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000003674 animal food additive Substances 0.000 claims abstract description 3
- 235000015097 nutrients Nutrition 0.000 claims abstract description 3
- 239000002699 waste material Substances 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 3
- 235000019728 animal nutrition Nutrition 0.000 claims description 2
- 239000000654 additive Substances 0.000 abstract description 2
- 230000000996 additive effect Effects 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 244000144972 livestock Species 0.000 abstract 2
- 235000016709 nutrition Nutrition 0.000 abstract 2
- 230000035764 nutrition Effects 0.000 abstract 2
- 235000013305 food Nutrition 0.000 abstract 1
- 239000002028 Biomass Substances 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001527609 Cryptococcus Species 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 241000223230 Trichosporon Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- SRBFZHDQGSBBOR-SQOUGZDYSA-N beta-D-arabinopyranose Chemical compound O[C@@H]1CO[C@@H](O)[C@@H](O)[C@@H]1O SRBFZHDQGSBBOR-SQOUGZDYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-KKQCNMDGSA-N beta-D-xylose Chemical compound O[C@@H]1CO[C@@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-KKQCNMDGSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000012978 lignocellulosic material Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Riešenie sa týká přísady do krmných zme- sí pre výživu hospodářských zvierat. Podstata přísady do krmných zmesi pre výživu hospodářských zvierat pripravitetná mikro- biálnou kultiváciou odpadného xylózového sirupu a živných solí mikroorganizmom Candida utilis spočívá v tom, že obsahuje 52,50 % hmot. bielkovin, 4,22 % hmot. lipi- dov, 33,26 % hmot. sacharidov, 6,38 % hmot. organických solí a 3,64 % hmot. bezdusíka- tých látok. Rešenie má použitie v potnohos- podárstve.The solution relates to an additive to feed feed for livestock nutrition. The essence of the feed additive ingredient for livestock nutrition prepared by microbial cultivation of xylose syrup and nutrient salts with Candida utilis is that it contains 52.50 wt. % protein, 4.22 wt. % lipids, 33.26 wt. % carbohydrates, 6.38 wt. % organic salts and 3.64 wt. nitrogen-free substances. The solution is used in the food industry.
Description
Vynález sa týká přísady do krmných zrpej t( sí pre výživu hospodářských zvierát ptiptá- venej mikrobiálnou cestou. ' ' * v ·.' *The invention relates to a feed additive for zrpej t (the network farm animal ptiptá- Veneya microbial means. '' * · In. '*
Je známe, že mikroorganizmy sú schopné využívat, sacharidy ako zdroj uhlíka K prípta- ( ve mikrobiálně] biomasy. Nedostatok tradičných surovin vyvolal záujem o spracovanie druhotných lignocelulózových materiálov a ich degradačných produktov na substráty pre fermentačnú výrobu. Schopnosť utilizácie týchto produktov bola študovaná u kvasiniek a kvasinkovltých mikroorganizmov patriacich do rodov Auerobasidium, Trichosporon, Candida Cryptococcus [Y. C. Han,It is known that microorganisms are able to utilize carbohydrates as a carbon source for biomass, and the lack of traditional raw materials has prompted interest in the processing of secondary lignocellulosic materials and their degradation products into substrates for fermentation production. and yeast-yellow microorganisms belonging to the genera Auerobasidium, Trichosporon, Candida Cryptococcus [YC Han,
C. E. Dunlap, C. D. Callihan: Food. Techn., 25, 32 (1971), J. Hojnoš, M. Bogdanová, R. Boríšek: Papír a celulóza 27, 36 (1972), J. Barta: Medz. Symp. Fed. Eur. mikrob. Spol. Viedeň (1977).C. E. Dunlap, C. D. Callihan: Food. Techn., 25, 32 (1971), J. Hojnoš, M. Bogdanová, R. Boríšek: Paper and Cellulose 27, 36 (1972), J. Barta: Medz. Symp. Fed. Eur. microbe. Spol. Vienna (1977).
Podstata přísady do krmných zmesí pre výživu hospodářských zvierat pripravitetná mikrobiálnou kultiváciou odpadného xylózového sirupu a živných solí mikroorganizmom Candia utilis spočívá v tom, že obsahuje 52,50 % hmot. bielkovín, 4,22 % hmot. lipidov, 33,26 % hmot. sacharidov, 6,38 °/o hmot. anorganických solí, 3,64 % hmot. bezdusíkatých látok.The essence of an ingredient in compound feed for animal nutrition prepared by microbial cultivation of waste xylose syrup and nutrient salts by the microorganism Candia utilis consists in that it contains 52.50 wt. protein, 4.22 wt. % of lipids, 33.26 wt. of carbohydrates, 6.38% w / w. % inorganic salts, 3.64 wt. nitrogen-free substances.
Kvasinky ako přísada do krmných zmesí majú tú výhodu, že obsahujú antioxidačné látky a zabraňujú tuchnutiu lipidových zložiek krmív. Majú vysoký obsah vitamínov skupiny B a aminokyselin. Aminokyselinové zloženie je podobné živočišným bielkovinám s výnimkou síru obsahujúcich aminokyselin. Výhodou uvedeného kmeňa je, že nie je patogény a že celkový obsah dusíkatých látok je 51,25 % hmot. v sušině. Ďalšou výhodou je že sa užije odpad vznikajúci pri výrobě D-xylózy k príprave mikrobiálnej biomasy, čím sa rieši aj otázka ekologická a využitie ekonomicky výhodných surovin. Dosiahne sa niekotkonásobné zhodnotenie přeměnou glycidového substrátu na bielkovinný. Tento substrát nebol využívaný uvedeným kmeňom k príprave mikrobiálnych bielkovín.Yeast as an additive to compound feed has the advantage of containing antioxidants and preventing the lipid components of the feed from decaying. They have a high content of B vitamins and amino acids. The amino acid composition is similar to animal proteins except for sulfur-containing amino acids. The advantage of said strain is that it is not pathogenic and that the total nitrogen content is 51.25% by weight. in dry matter. Another advantage is that the waste resulting from the production of D-xylose is used for the preparation of microbial biomass, which also addresses the ecological issue and the use of economically advantageous raw materials. Multiple evaluations are achieved by converting the carbohydrate substrate to protein. This substrate was not used by the strain to prepare microbial proteins.
Příklad 1Example 1
Připraví sa kultivačně médium, na 1 liter sa použije 3 g síranu amonného, 0,5 g kyslého fosforečnanu draselného, 1 g síranu horečnatého a 1 g kvasničného autolyzátu. Do kultivačného média sa přidá sirup zostávajúci ako odpad pri výrobě D-xylózy obsahujúci 61 % hmot. sacharidov. Tieto sa, «h|aridy? sú zastúpené 44,91 hmot. D-xyíózy, 19,P/o hmot.' Ů-glukózy, 11,63 °/o hmot. ů-arabinózý-a Ď-xylózy, 10,64 % hmot. D-galaktózy, 8, 14 % hmot. D-manózy, 3,02 % hrtíbtt K-rafhnÓzy, 1,86 % hmot. D-ribózy. Sirup sa přidával v množstve, aby sme dosiahli obsah redukujúcich látok v médiu 20 g na liter. Sterilně kultivačně médium sa zaočkuje suspenziou kvasiniek Candida utilis 8.10e a kultivuje na recipročnej trepačke s počtom kyvov 108 za min pri teplote 25 °C po dobu 3 až 4 dní, potom sa biomasa odstředí, premyje vodou a vysuší. Výťažok biomasy u uvedeného kmeňa bol 6,720 g sušiny na liter kultivačného média a pH pri kultivácii je 4,8. Získaná biomasa obsahuje 52,50 % hmot. bielkovín, 4,22 % hmot. lipidov, 33,26 °/o hmot. sacharidov a 6,38 % hmot. anorganické soli, bezdusíkaté látky 3,64 % hmot. na základnú hmotnost biomasy. Patogenita sa zisťovala na myšiach o hmotnosti 19 až 22 g, ktoré boli z chovnej stanice Dobrá voda. Suspenzia buniek Candida utilis v množstve 0,5 ml bola intravenózne píchaná v priebehu 35 dní [J. Šandula, S. Kocková-Kratochvílová, M. Zámečníkové: Folia Microbiologica 8, 313 (1963) ]. Pri testovaní uvedeným kmeňom neuhynul ani jeden jedinec.Culture medium is prepared, 3 g of ammonium sulfate, 0.5 g of potassium phosphate acid, 1 g of magnesium sulfate and 1 g of yeast autolysate are used per liter. Syrup remaining as waste in the production of D-xylose containing 61 wt. carbohydrates. These are, «h | aridy ? are represented by 44.91 wt. D-xylose, 19, P / wt. Δ-glucose, 11.63% w / w % β-arabinose and β-xylose, 10.64 wt. D-galactose, 8, 14 wt. % D-mannose, 3.02 wt% K-rafnose, 1.86 wt. D-ribose. The syrup was added in an amount to achieve a reducing agent content of 20 g per liter. The sterile culture medium is inoculated with a suspension of yeast Candida utilis 8.10 e and cultured on a reciprocating shaker at 108 rpm at 25 ° C for 3-4 days, then the biomass is centrifuged, washed with water and dried. The biomass yield of said strain was 6.720 g dry matter per liter of culture medium and the pH of the culture was 4.8. The biomass obtained contains 52.50 wt. protein, 4.22 wt. % of lipids, 33.26% w / w; % of carbohydrates and 6.38 wt. inorganic salts, nitrogen-free substances 3.64 wt. to the basis weight of biomass. Pathogenicity was detected in mice weighing 19-22 g, which were from the Dobrá voda breeding station. A 0.5 ml Candida utilis cell suspension was injected intravenously over 35 days [J. Sandula, S. Kockova-Kratochvilova, M. Zamecnikova: Folia Microbiologica 8, 313 (1963)]. Neither individual died when tested with the strain.
Příklad 2Example 2
Postupuje sa ako v příklade 1 s tým rozdielom, že sa použije kultivačně médium: 1, 4 g močoviny, 3 g kyslý fosforečňan draselný, 0,2 g síran horečnatý a 0,1 ml mikroelementovaného roztoku. Teplota kultivácie je 31 °C a pH 5,0. Výťažok u uvedeného kmeňa bol 7,525 g sušiny na 1 liter kultivačného média. Získaná biomasa obsahuje 52,50 % hmot. bielkovín, 4,22 % hmot. lipidov, 33,26 % hmot. sacharidov a 6,38 % hmot. anorganické soli, bezdusíkaté látky 3,64 % hmot. na základnú hmotnost biomasy.The procedure is as in Example 1 except that the culture medium is: 1.4 g urea, 3 g potassium phosphate acid, 0.2 g magnesium sulfate and 0.1 ml microelemented solution. The culture temperature is 31 ° C and pH 5.0. The yield for said strain was 7.525 g dry matter per liter of culture medium. The biomass obtained contains 52.50 wt. protein, 4.22 wt. % of lipids, 33.26 wt. % of carbohydrates and 6.38 wt. inorganic salts, nitrogen-free substances 3.64 wt. to the basis weight of biomass.
Příklad 3Example 3
Postupuje sa ako v příklade 1 s tým rozdielom, že pH je 5,5 a teplota kultivácie 30 °C. Získaná biomasa obsahuje 52,50 % bielkovín, 4,22 % hmot. lipidov, 33,26 % hmot. sacharidov a 6,38 % hmot. anorganické soli, bezdusíkaté látky 3,64 °/o hmot. na základnú hmotnost biomasy.The procedure is as in Example 1 except that the pH is 5.5 and the culture temperature is 30 ° C. The biomass obtained contains 52.50% protein, 4.22% wt. % of lipids, 33.26 wt. % of carbohydrates and 6.38 wt. inorganic salts, nitrogen-free substances 3.64% w / w; to the basis weight of biomass.
Výroba móže nájsť široké použitie v pol'nohospodárstve pri príprave krmív pre hospodářské zvieratá.Production can find widespread use in agriculture in the preparation of animal feed.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS848916A CS244335B1 (en) | 1984-11-21 | 1984-11-21 | Fodder mixtures' admixture for feeding of domestic animals |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS848916A CS244335B1 (en) | 1984-11-21 | 1984-11-21 | Fodder mixtures' admixture for feeding of domestic animals |
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| Publication Number | Publication Date |
|---|---|
| CS891684A1 CS891684A1 (en) | 1985-09-17 |
| CS244335B1 true CS244335B1 (en) | 1986-07-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS848916A CS244335B1 (en) | 1984-11-21 | 1984-11-21 | Fodder mixtures' admixture for feeding of domestic animals |
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| CS (1) | CS244335B1 (en) |
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1984
- 1984-11-21 CS CS848916A patent/CS244335B1/en unknown
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| Publication number | Publication date |
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| CS891684A1 (en) | 1985-09-17 |
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