CS233109B1 - Method for determining the content of N- (2,2,2-trichloro-1-morpholinoethyl) formamide - Google Patents
Method for determining the content of N- (2,2,2-trichloro-1-morpholinoethyl) formamide Download PDFInfo
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- CS233109B1 CS233109B1 CS453783A CS453783A CS233109B1 CS 233109 B1 CS233109 B1 CS 233109B1 CS 453783 A CS453783 A CS 453783A CS 453783 A CS453783 A CS 453783A CS 233109 B1 CS233109 B1 CS 233109B1
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Abstract
Vynález sa týká sposobu stanovenia obsahu N-(2,2,2-trichlór-l-morfolínoetyl) » formamidu na substrátech biologického pávodu plynovou chromatografiou. Presne navážené množstvo vzorky sa extrahuje organickým rozpúáladlom so známým množstvem zvoleného vnútorného átandardu, přečistí sa a po analýze plynovou chromatografiou aa obsah N-(2,2,2-trichlór- -1-morfolínoetyl) formamidu zistl porovnáváním chromatografického záznamu vzorky so záznamem roztoku obsahujúceho známe množstvá átandardu N-(2,2,2-trichlór-l-morfolínoetyl) formamidu a zvoleného vnútorného Standardu v organiokom rozpúátadleThe invention relates to a method for determining the content of N-(2,2,2-trichloro-1-morpholinoethyl)» formamide on substrates of biological origin by gas chromatography. A precisely weighed amount of the sample is extracted with an organic solvent with a known amount of the selected internal standard, purified and after analysis by gas chromatography, and the content of N-(2,2,2-trichloro-1-morpholinoethyl) formamide was determined by comparing the chromatographic record of the sample with the record of a solution containing known amounts of the standard N-(2,2,2-trichloro-1-morpholinoethyl) of formamide and the selected Internal Standard in an organic solvent
Description
- /- 233 109233/109
Vynález »a týká spdsobu stanovenia obsahu N-(2,2,2-tri-chlór-1-morfolínoetyl)formamidu na substrátoch biologickéhopovodu plynovou chromátografiou. Z literatúry nie sú známe spósoby stanovenia obsahuN-(2,2,2-trichlór-1-morfolínoetyl)formamidu (trimorfamidu) nasubstrátoch biologického pdvodu.The invention relates to a method for the determination of the content of N- (2,2,2-trichloro-1-morpholinoethyl) formamide on biochemical substrates by gas chromatography. There are no known methods for determining the content of N- (2,2,2-trichloro-1-morpholinoethyl) formamide (trimorphamide) by biological substrates.
Teraz sa zistilo, že je možné stanovit obsah trimorfami-du na substrátoch biologického povodu plynovou chromátografiouspčsobom podlá vynálezu.It has now been found that it is possible to determine the content of trimorphamid on the substrates of the biological phase by gas chromatography according to the invention.
Podstata vynálezu spočívá v tom, že sa presne naváženémnožstvo vzorky extrahuje organickým rozpúéfadlom so známýmmnožstvom zvoleného vnútorného Standardu, přečistí sa a po ana-lýze plynovou chromátografiou sa obsah trimorfamidu zistí po- t rovnávaním chromatografického záznamu vzorky so záznamom rozto-ku obsahujúceho známe množstvá Standardu trimorfamidu a zvole-ného vnútorného Standardu v organickom rozpúšťadle.The essence of the present invention is that the precisely weighed sample is extracted with an organic solvent having a known amount of internal standard selected, purified, and after gas chromatography analysis, the content of trimorphamide is determined by equilibrating the sample chromatographic record with a record of a solution containing known amounts of Trimorphamide Standard and the selected internal standard in an organic solvent.
Uasledujúci příklad objasňuje, ale neobmedzuje predmetvynálezu. Příklad 1The following example illustrates but does not limit the invention. Example 1
Do odmernej banky na 50 ml, so zábrusom sa navážilo 12,0 gjačmeňa namoreného trimorfamidom, zo zásobného roztoku sa při-dalo 25 ml metylénchloridu p.a. obsahujúceho v 1 ml 0,2 mg Stan-dardu n-butylesteru kyseliny 2-metyl-4-chlor-fenoxyoctovej.Into a 50-ml volumetric flask, with a ground-glass joint, trimmed with trimmer amide, 12.0 g / well, 25 ml of methylene chloride p.a. containing 0.2 mg of 2-methyl-4-chloro-phenoxyacetic acid n-butyl ester in 1 ml.
Banka sa pevne uzavřela zabrúsenou skleněnou zátkou a jej obsahsa extrahoval na stolněj trepačke 10 minút. Po skončení extrak-cie sa obsah banky přefiltroval do čistej suchéj odmernej banky,do ktorej sa přidalo 0,5 g bezvodého síranu sodného. Číry filt-rát sa dávkoval na kolonu plynového chromátografu a obsah tri- 2 233 109 morfamidu sa zistil kvantitativným porovnáním chromátografické-ho záznamu vzorky so záznamom metylénchloridového roztoku soznámým obsahom Standardu trimorfamidu a Standardu n-butyleate-ru kyseliny 2-metyl-4-chlór-fenoxyoctovej, zvolenej za vnútor-ný Standard.The flask was tightly sealed with a ground glass stopper and extracted with a bench shaker for 10 minutes. After extraction, the contents of the flask were filtered into a clean dry volumetric flask to which 0.5 g of anhydrous sodium sulfate was added. The clear filtrate was fed to a gas chromatograph column and the tri-233,210,109 morphamide content was determined by quantitative comparison of the chromatographic sample recording with the methylene chloride solution record with the known trimoramid and n-butyl ether standard 2-methyl-4-chlorine standard -phenoxyacetic acid, selected as Internal Standard.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS453783A CS233109B1 (en) | 1983-06-21 | 1983-06-21 | Method for determining the content of N- (2,2,2-trichloro-1-morpholinoethyl) formamide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS453783A CS233109B1 (en) | 1983-06-21 | 1983-06-21 | Method for determining the content of N- (2,2,2-trichloro-1-morpholinoethyl) formamide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CS233109B1 true CS233109B1 (en) | 1985-02-14 |
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ID=5388360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS453783A CS233109B1 (en) | 1983-06-21 | 1983-06-21 | Method for determining the content of N- (2,2,2-trichloro-1-morpholinoethyl) formamide |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS233109B1 (en) |
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1983
- 1983-06-21 CS CS453783A patent/CS233109B1/en unknown
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