CS231374B1 - Equipment for oocythe withdrawal - Google Patents

Abstract

The subject of the invention is a collection kit oocytes from ovarian or farmed ovaries animals. The set consists of metal cannula on which it is directly threaded plastic sampling tube, expensive the end is airtight through the stopper which stopper is threaded through also suction tube, fitted out end clutch to connect the kit to a vacuum source. All set it is kept airtight until used packaging and sterilized. The kit is suitable for laparoscopic oocyte collection or laparotomy for diagnostic or for purposes pregnancy by method of fertilization outside orga » nism.

Description

Oocyte collection kit

The present invention provides a kit for collecting oocytes from the ovary of a female or livestock animal. The kit consists of a metal cannula, onto which a plastic sampling hose is directly threaded, the expensive end of which is airtightly pushed through the plug, and the suction tube is also airtightly pushed through the plug, provided with a coupling at the far end to connect the kit to the vacuum source. The entire kit is stored in an airtight container and sterilized until use.

The kit is suitable for oocyte retrieval by the laparoscopic or laparotomic route for diagnostic purposes or to achieve pregnancy by fertilization outside the organism.

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The invention provides for the construction of a disposable oocyte collection kit from a female ovary or livestock.

Prior art devices are assembled from parts before use. Most of these prior art devices have blind spaces in which the oocyte adheres, substantially reducing the yield of oocyte recovery. Blind spaces arise at the junctions of the components, mainly through conical cavities and cones common to injection needles and syringes. Further, there are known devices operating a Teflon tube passing tightly through the injection needle and opening without any passage to the collection container. The construction of this device is quite demanding and is not suitable for use as a disposable set.

The drawbacks of the prior art oocyte collection devices are overcome by the kit of the invention. SUMMARY OF THE INVENTION It consists of a metal cannula on which a plastic collection hose is directly threaded, the other end of which is airtightly passed through the plug of the collecting container, which plug is also airtightly connected to the suction hose provided at the opposite end with a coupling vacuum. The transition from the cannula to the plastic tubing is thus solved by directly attaching the tubing to the cannula so that no blind space is created.

The very simple design, mainly made of plastic and easy to sterilize, gives the kit a disposable, safely sterile and ready to use feature.

In the assays series, 100% efficiency of retrieving oocytes from mature follicles was achieved in 98 donations. Alike

- 2,231,374 results have only been published for equipment that contained a Teflon tube that directly passed through the tight injection needle and which also resulted without any transition to the collection container. With this comparable device, 100% efficacy was achieved at 39 samples. For other known devices, a maximum efficiency of 56% was achieved.

The invention and its effects are explained in more detail in the description of an exemplary embodiment thereof by means of the attached drawing, in which the kit is shown schematically.

The oocyte collection kit according to the invention consists of a metal cannula 1 with a sharp tip and a handle 2. On its blunt end 3 a plastic collection tube 4 is threaded, which passes tightly through the plastic plug 5. A tightly suction hose 6 made of plastic, provided with a coupling 7 for connection to a vacuum source, also extends through this plug 5. The plug 5 is fitted with a collection container 8 before use, which can be replaced during the procedure.

The device operates in such a way that the tip of the cannula 1 and the cannula, respectively, is the tip of the cannula during surgery. needles impaled follicle, arching from the surface of the ovary. The kit is connected to a vacuum source by a coupling 7. As a result of the vacuum, the oocyte is torn away from the follicle wall and, together with the fluid contained in the follicle, is transported through the lumen of the cannula 1 and the collection tube 4 into the collection container 8 without encountering a blind space where it could become stuck. The whole kit is sterilized and stored in a hermetic package until use.

The oocyte collection kit of the invention is intended for oocyte collection from a female or livestock ovary by the laparoscopic or laparotomic route for diagnostic purposes or to achieve pregnancy by a method of fertilization outside the parent organism. The overall arrangement, and especially because it is designed as a one-off aid, excludes transmission of infection during surgery. The high recovery in oocyte recovery substantially reduces the need to repeat the procedure. It eliminates oocyte damage caused by residues of water or toxic substances that may remain in the kit reused.

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SUBJECT MATTER

Claims (1)

Oocyte collection kit, characterized in that it consists of a metal cannula (1) on which a collection tube (4) is threaded, the other end of which is airtightly passed through the plug (5), and through the plug (5) the suction tube is also airtight. 6), provided at the opposite end with a coupling (7) for connecting the kit to a vacuum source. 1 Drawing