CS214232B1 - Diluent of boar's sperm - Google Patents

Description

The present invention provides a composition of a preservative diluent for boar semen for use in the insemination of sows.

The current short-term preservation of boar semen in the liquid state is carried out by diluting the semen with an aqueous solution containing substances which have a beneficial effect on maintaining osmotic pressure, energy reserves and a preservative effect. A disadvantage of the existing preservative diluents is the low viability of sperm boars.

The above-mentioned deficiency solves the boar semen diluent according to the invention which is based on the active substances sodium glutamate, calcium chloride, magnesium chloride and primary sodium phosphate.

Using a new boar sperm diluent has a higher effect against known diluents, characterized by a 15-30% higher sperm survival, depending on the retention period, which in turn results in a significant increase in sows' marrowing average of $ 08.

The boar semen diluent according to the invention is applied in the same way as the known diluent, with the addition of hen's egg yolk and antibiotics.

Example: to a solution obtained by mixing distilled water boiled 1,000.00 g sodium citrate neutral: 3i »60 g anhydrous glucose 9.00 g slufanilamide 3.00 g potassium chloride 0.21 g sodium chloride 0.14 g sodium glutamate () , 30 g calcium chloride free 2.80 g magnesium chloride 0.37 g primary sodium phosphate 0.03 g add 3 # hen egg yolk, 730.000 m, pehicillin, 0.3 g Steptomycin and the preservative diluent thus prepared is added to fresh semen so that the dose volume of the semen is at least 3.5. 10 progressive sperm. The undiluted semen is stored at an optimum temperature of 19 ° C to 16 ° C, and both the inseminated and the reinserrtittae are carried out in the usual naphthalated manner within 48 hours after collection and dilution. When applied according to the above instructions, the period I. - IX. month 1980 obtained results of UV6detté in tables 1 and 2 *

Table i - Results of comparative testing of efficacy of NG diluent after I. inseminadi 8 with SAD diluent

Thinner

Number

i

Out of it

Percentage of pregnancy

NG 3,941 2,749 69.7

SET 3 978 2 43I 61.6

Difference in favor of NG diluent: +8.1

Table 2 - Results of comparative testing of the efficacy of the new diluent Ng after all inseminations performed with SAD diluent

Thinner Number Of which Percentage

Even, the insemination was pregnant

NG 4,475 3,190 71.2

SAD 4,603 2,902 63.0

Difference in favor of NG diluent: +8.2

In Tables 1 and 2, the symbols used have the following meanings:

NG - new thinner according to the invention 'SAD - thinner nationwide used in Czechoslovakia according to:

SADOVNIKOVA, Μ. T., 1966: Protecting the Seeds of Khrynodos without Chloroagents, Shvevod 20: 5-28.

Claims (1)

Diluent of boar semen with an aqueous solution containing sodium citrate neutral, anhydrous glucose, sulfanilamide, potassium chloride, sodium chloride, characterized in that it contains sodium glutamate, calcium chloride, magnesium chloride and primary sodium phosphate as active ingredients.