CS201807B1 - Strain of aspergillus niger s 59 1600 - Google Patents

Description

The invention relates to a novel high-production strain S 59 for the submersible production of organic acids for use in particular in food chemistry and pharmacology.

For the submersible production process for the production of organic acids, for example citric acid, production strains suitable for production by the surface method cannot be used and new strains need to be bred and applied. This situation is partially addressed by MS. No. 196 001 using the newly bred Aspergillus niger 97 A E 81 and NG 233 mutants for the submerged citric acid production. However, the specificity in the sensitivity of the individual mutants to the basic fermentation conditions remains unresolved, without which economic production cannot be achieved without knowledge. This precludes applying the same technology using different production strains.

The subject of the invention is a strain of Aspergillus niger S 59, a high-production mutant for the production of organic acids by submersal fermentation, deposited in the collection of microorganisms at the Department of Botany, Faculty of Science, Charles University, Prague 2, under number 1600.

Morphological and physiological characteristics of Aspergillus niger S 59 mutant

Thom, Ch., Raper, K. B .: Aspergillie Manual, Baltimore, 1945:

Strain S 59 grows well and sporulates on wort agar. Disputes are black-brown. On sporulating agar with beer, it creates colonies about 4.5 cm in diameter with dense black sporulation in five days, the underside of the colonies is colored yellow. On Raulin agar, colonies are small, up to 2 cm in diameter, sporulation is sparse, spores are ocher colored, and colonies are significantly wrinkled. On Czapka's agar, a 4 cm trunk forms colonies with weak ocher sporulation, wrinkled, yellow on the underside.

Microscopic appearance: spores are dark brown, hairy 3.5 to 4.5 μ in diameter, conidiophores 0.5 to 0.7 mm, high, heads with a diameter of 0.1 mm markedly round. The shelf life of the spores is long-term, for several years when stored in carboraffin or in lyophilized state. During submersive cultivation on synthetic soil with 10 to 20% sucrose, convert the S 59 mutant from 65 to 75% sucrose to citric acid. The mutant is susceptible to a deficiency or excess of trace elements, especially Fe in the medium, whose deficiency or excess results in a significant decrease in citric acid production.

The S 59 mutant was obtained by UV light on a protoplast suspension of the original A. niger K 10 strain so that the number of surviving individuals was less than 10%. Proto201807 plastics were prepared by disrupting the cell walls of young hyphae A, niger K 10 enzyme complexes from snail stomachs. The stability of the released protoplasts was ensured by the presence of stabilizers such as KCl or NaCl, some sugars or higher alcohols along with a small amount of Ca ¹ *. The protoplast suspension was poured onto sporulated agar media containing stabilizers. After spore agar spores were washed, diluted and subjected to monocolonium selection. Individual insulators were evaluated for their ability to grow, sporulate and produce citric acid. The best isolate designated Q. niger S 59 is the subject of the invention.

Claims (1)

Aspergillus niger strain S 59 1600, a high-production mutant for the production of organic acids by submersal fermentation, deposited in the collection of microorganisms at the Department of Botany, Faculty of Science, Charles University, Prague. No. 1600.