CS201110B1 - Paper of the testing set for the screening diagnosis of bacteryureids - Google Patents
Paper of the testing set for the screening diagnosis of bacteryureids Download PDFInfo
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- CS201110B1 CS201110B1 CS91176A CS91176A CS201110B1 CS 201110 B1 CS201110 B1 CS 201110B1 CS 91176 A CS91176 A CS 91176A CS 91176 A CS91176 A CS 91176A CS 201110 B1 CS201110 B1 CS 201110B1
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- CS
- Czechoslovakia
- Prior art keywords
- paper
- bacteriuria
- screening
- urine
- bacteryureids
- Prior art date
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- 238000012216 screening Methods 0.000 title claims description 9
- 238000003745 diagnosis Methods 0.000 title description 6
- 201000004538 Bacteriuria Diseases 0.000 claims description 11
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 239000005018 casein Substances 0.000 claims description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 2
- 235000021240 caseins Nutrition 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 235000020183 skimmed milk Nutrition 0.000 claims description 2
- 239000001488 sodium phosphate Substances 0.000 claims 1
- 229910000162 sodium phosphate Inorganic materials 0.000 claims 1
- 239000002689 soil Substances 0.000 claims 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims 1
- 210000002700 urine Anatomy 0.000 description 13
- 239000007788 liquid Substances 0.000 description 6
- 244000005700 microbiome Species 0.000 description 5
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 208000026723 Urinary tract disease Diseases 0.000 description 2
- 208000012931 Urologic disease Diseases 0.000 description 2
- 239000003708 ampul Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 229920001169 thermoplastic Polymers 0.000 description 2
- 239000004416 thermosoftening plastic Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 230000002485 urinary effect Effects 0.000 description 2
- 208000014001 urinary system disease Diseases 0.000 description 2
- 240000004160 Capsicum annuum Species 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000588769 Proteus <enterobacteria> Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000001511 capsicum annuum Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229920006352 transparent thermoplastic Polymers 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
(54) PAPIERIK TESTOVACEJ SÚPRAVY PEE SCREENINGOVÉ DJjAGNOSTIKOVANIE BAKTERIURIE(54) PAPER TEST KIT PEE SCREENING DJACTERIZATION OF BACTERIURY
Předmetom vynálezu je papierik testovacej súpravy pre screeningové diagnostikovanie infekčného ochorenia močových ciest - bakteriurie.The subject of the invention is a test kit paper for screening for the diagnosis of infectious urinary tract disease - bacteriuria.
Diagnostikovanie bakteriurie sa uekutočňuje mikrobiologickým vyšetřením moča klasickou kultiváciou na platniach s příslušnými agarovými živnými pódami. Toto vyšetrenie je však značné pracné a materiálně náročné, čo stažuje možnost takéhoto vyšetrenia vo všetkých prípadoch podozrenia z bakteriurie. Z toho dóvodu je vyšetrenie celého súboru podozrivého materiálu možné len pomocou výběrového screeningového testu, ktorého úlohou je jednoduchým sposobom identifikovat pozitivně případy z celého súboru podozrivého materiálu a tým podstatné znížit počet přesných vyšetření klasickou plátnovou metodou. Tento test by mal byt pracovně a materiálfie nenáročný a mal by 'poskytnut aspoň přibližná kvantitativná představu o výskyte mikroorganizmov vo vzorke moča.The diagnosis of bacteriuria is carried out by microbiological examination of urine by classical cultivation on plates with appropriate agar nutrient platforms. However, this examination is laborious and materially demanding, making it difficult to investigate in all cases of suspected bacteriuria. For this reason, the examination of the entire set of suspect material is possible only by means of a selective screening test, whose task is to identify positively cases from the entire set of suspect material in a simple manner and thereby substantially reduce the number of accurate screening tests. This test should be laborious and materially demanding and should provide at least an approximate quantitative idea of the presence of microorganisms in the urine sample.
V súčasnej době existuje značné množstvo róznych screeningových testov na bakteriúriu, pracujúcich na najroziičnejších princípoch. Medzi najrozšírenejáie patria testy založené na principe kultivácie mikroorganizmov moča na vrstvičkách agarovej pody na vhodnéj podložke, ktoré boli ponořené krátko do vzorky moča. Výroba takýchto testov je náročná. Ďaláia skupina testov spočívá na principe kultivácie patogénnych mikroorganizmov moča na prúžkoch papiera 8 dehydratovanou živnou podou. po ponoření do vzorky moča. Tieto papieriky majú indikátorový systém, ktorý farebno registruje produkty uiikrobiálneho metabolizmu počas rastu. PřevážnáAt present, there are a number of different bacteriuria screening tests working on the most diverse principles. Among the most widespread are assays based on the principle of culturing urine microorganisms on agar bed layers on a suitable substrate that have been immersed briefly in a urine sample. The production of such tests is difficult. Another group of tests is based on the principle of culturing pathogenic urinary microorganisms on strips of paper 8 with a dehydrated nutrient medium. after immersion in urine samples. These papers have an indicator system that color-cues the products of uiicrobial metabolism during growth. The vast
201 110 vačšina týchto testov nevyžaduje riedenie moče a kvantitativný údaj sa získá přibližným posúdením hustoty porastu. Vzhíad porastu se však líši podía kultivovaného kmeňa, čo slažuje kvantitativný odhad. Bol vyvinutý testovací papierik na atanovenie patogénnej mikroflóry moča autormi Mašek a kol., chráněný patentovým spieom 120942, ktorý je určený na testovanie neriedeného moča. Tento papierik neregistruje jednoznačné celú potrebnú škálu kmenov spásobujúcich bakteriálně ochorenie močových ciest. Okrem toho, pri vyššej koncentrácii moča pozitívneho bakteriúriou dochádzalo k falošne negativným výsledkom, pretože rast mikroorganizmov pri koncentrácii 10^ až 10? zárodkov v jednom mililitr! testovanej kvapaliny sa neprejavil farebnou změnou papierika.201 110 most of these tests do not require urine dilution and a quantitative data is obtained by roughly assessing the stand density. However, the appearance of the crop differs according to the cultivated strain, which weakens the quantitative estimate. Test paper for the detection of pathogenic urine microflora by Mašek et al., Patent protected 120942, designed to test undiluted urine has been developed. This paper does not clearly identify the entire range of strains causing bacterial urinary tract disease. In addition, at a higher urinary bacteriuria-positive urine concentration, false negative results occurred because the growth of microorganisms at a concentration of 10 to 10? germs in one milliliter! the test liquid did not show a color change of the paper.
Podstata papierika testovacej súpravy pre screeningové diagnoatikovanie bakteriúrie, impregnovaného dehydratovanou živnou podou na báze vody, odstředěného mlieka, hydrolyzátu kaseinu, kvasničného autolyzátu, peptonu a agaru, podía. vynálezu spočívá v tom, že podá, ktorou je papierik naimpregnovaný, obsahuje sekundárný fosforečnan sodný v množstve 4 až 14 g a 2,3,5trifenyltetrazolium chlorid v množstve 0,4 až 0,7 g v litri pády.The essence of a test kit for screening diagnosis of bacteriuria impregnated with a dehydrated nutrient base based on water, skim milk, casein hydrolyzate, yeast autolysate, peptone and agar, respectively. According to the invention, the administration by which the paper is impregnated contains secondary sodium phosphate in an amount of 4 to 14 g and 2,3,5-triphenyltetrazolium chloride in an amount of 0.4 to 0.7 g per liter of fall.
Toto zioženie papierika %aručuje rast prakticky všetkých kmenov sposobujúcich bakteriúriu, tj. E.coli, Enterobaeter, Próteus, Pseudomonas, Staphyiociccus, Streptococcus, a dalších, a to v prakticky neobmezenej škále koncentrácie. Pre uíahčenie odčitania kvantitativného údajů, ktorý je rozhodujúcim u screeningového testu pre posúdenie pozitivněj bakteriúrie, papie-r rik Dodía vynálezu je zaradený ako aúčasl súpravy pre diagnostikovanie bakteriúrie, ktoré umožňuje riedenie vzorky moča tak, aby po naočkovaní papierika v tomto riedení, a po jeho kuitivácii, bolo možné spočítáním farebných Skvrn na papieriku stanovil počet živých zárodkov v objeme východzej vzorky, lestovacia súprava móže pozostávat z ampulky so sterilným riediacím roztokom, zo sterilného kvapadia a tyčinky určenej k zamiešaniu roztoku, zabalených v hermeticky uzavretom vrecúšku, a zo sterilného prážku papiera impregnovaného dehydratovanou živnou podou podía vynálezu v hermeticky uzavretom vrecúšku z termoplastickéj priesvitnej folie. Spoaob použitia testu je nasledujúci: Pomocou kvapadia sa do ampulky s riediacim roztokom kvapné příslušný počet kvapiek čerstvého zamiešaného moča, pre dosiahnutie požadovaného riedenia moča a obsah ampulky sa premieša pomocou tyčinky. Po rozstrihnuti vrecúška sa papierik vyberie pomocou sterilněj pinzety a ponoří sa do kvapaliny v ampulke. Papierik sa zbaví prebytočnej kvapaliny, vloží spát do vrecúška, hermeticky sa v ňom uzavrie a injkubuje sa 24 až 48 hodin pri 37 °C. Po tejto době sa spočítajú na papieriku vzniklé Skvrny.This loading of paper guarantees the growth of virtually all strains causing bacteriuria, i. E. coli, Enterobaeter, Proteus, Pseudomonas, Staphyiociccus, Streptococcus, and others, in a virtually unlimited concentration range. To facilitate the reading of the quantitative data that is critical in the screening test for assessing positive bacteriuria, the paprika Dodi invention is included as part of a bacteriuria diagnosis kit that allows the urine sample to be diluted after inoculation of the paper at that dilution. The staining kit may consist of an ampoule with sterile diluent, a sterile liquid and a solution-mixing stick packed in a hermetically sealed sachet, and a sterile paper powder. impregnated with a dehydrated nutrient according to the invention in a hermetically sealed bag of thermoplastic translucent film. The method of use of the test is as follows: Using a dropper, the appropriate number of drops of fresh mixed urine is dripped into the diluent vial to achieve the desired urine dilution and the contents of the vial are mixed with a stick. After cutting the sachet, the paper is removed with sterile tweezers and immersed in the liquid in the vial. The paper is drained of excess liquid, placed back in the bag, hermetically sealed therein and injected at 37 ° C for 24 to 48 hours. After this time, the spots formed on the paper are counted.
Papierik s dehydratovanou živnou podou:Dehydrated nutrient paper:
Vo vrecúšku z bezfarebnej priesvitnej termoplastickéj fólie je hermeticky uzavretý steriiný prúžok bieleho papiera, rozmerov umožňujúcich jeho ponorenie do kvapaliny v ampulke bez antimlkrobiálnych vlastností, ktorý je impregnovaný dehydratovanou živnou podou s indikátorovým systémom pre identifikáciu rastu mikroorganizmov, zioženia uvedeného v predmete vynálezu.A colorless transparent thermoplastic film bag is hermetically sealed with a sterile strip of white paper, sized to immerse in a liquid in an ampoule without antimlrobial properties, impregnated with a dehydrated nutrient tray with an indicator system for identifying the growth of the microorganisms of the invention.
Riediaci roztok:Dilution solution:
Sterilný izotonický roztok fosfátového pudru, například tohto zioženia: 6,15 g sekundárného fosforečnanu sodného a 3,17 g primárného fosforečnanu draselného v jednom litri deštilo201 110 vanej vody o pH 7,0 až 7,2, je hermeticky uzavretý v sklenenej ampulke.A sterile isotonic phosphate powder solution such as the following: 6.15 g of secondary sodium phosphate and 3.17 g of primary potassium phosphate in one liter of rain water at pH 7.0-7.2 is hermetically sealed in a glass vial.
Kvapadlo a tyčinka:Dropper and stick:
Vo vrecúšku z termoplastickéj fólie je hermeticky uzavretá sterilná tyčinka, ktorá rozmermi a mechanickou vlastnosíou umožní zamiešat roztok v ampulke a sterilně kvapadlo s gumovou savičkou.The thermoplastic foil pouch has a hermetically sealed sterile bar which, by dimensions and mechanical properties, allows the solution to be mixed in the vial and the sterile dropper with the rubber teat.
Papierik testovacej súpravy pre diagnostikovanie bakteriúrie, pře svoje vhodné vlastnosti Čo do schopnosti registrácie patogénnych mikroorganizmov móže byí samotný, připadne ako súčsst súpravy umožňujúcej riedenie vzorky, použitý pre orientačné stanovenie kontaminácie a iných kvapalín než je íudský moč, a to například iných tekutin mikrobiologicky analyzovaných v humánněj alebo veterinárněj medicíně, produktov potravinářského nápojárskeho priemyslu, kvapalných vzoriek charakterizujúcich hygienické poměry (výplachy) apod.The test kit paper for the diagnosis of bacteriuria, due to its suitable properties Regarding the ability to register pathogenic microorganisms, it may itself be used as part of the sample diluent kit used for the indicative determination of contamination and non-human urine fluids, for example other fluids microbiologically analyzed. human or veterinary medicine, food beverage industry products, liquid samples characterizing hygiene conditions (washings), etc.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS91176A CS201110B1 (en) | 1976-02-12 | 1976-02-12 | Paper of the testing set for the screening diagnosis of bacteryureids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS91176A CS201110B1 (en) | 1976-02-12 | 1976-02-12 | Paper of the testing set for the screening diagnosis of bacteryureids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CS201110B1 true CS201110B1 (en) | 1980-10-31 |
Family
ID=5342171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS91176A CS201110B1 (en) | 1976-02-12 | 1976-02-12 | Paper of the testing set for the screening diagnosis of bacteryureids |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS201110B1 (en) |
-
1976
- 1976-02-12 CS CS91176A patent/CS201110B1/en unknown
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