CS200083B1 - Antifoam agent for fermentation processes - Google Patents

Description

(54)

Defoaming agent for fermentation processes

The invention relates to a new antifoaming agent for fermentation processes.

In the cultivation of microorganisms in intensively ventilated or mixed media, strong foaming occurs. Some surfactants, such as humic acid, proteins, degradation products of proteins and screws, pectins and saponins found in nutrient soils, cause intense foam formation. Other surfactants arise from the nutrient media during sterilization of the media and as a result of metabolism during fermentation. During fermentation, the formation of feathers must be reduced because, due to intensive foaming, local overheating, release of cellular protein, insufficient supply of nutrients to the cells, damage to the production microorganisms by autolysis, thereby reducing the yield of the fermentaoe product, reducing the carbon dioxide circuit and aeration efficiency , it makes it difficult for the medium to mature independently and does not achieve the required degree of concentration.

A number of mechanical devices have been proposed for the removal of foam in fermentation processes which, however, do not solve the problem comprehensively, and thus the most widespread method of foam removal consists in the use of surface active chemicals. The best known antifoams are vegetable oils, animal fats, their fatty acids, either alone or in admixture with mineral oil. Further, glycols, amides and amines of fatty acids, organic phosphates, condensation products of ethylene oxide with stearin, various mixtures, the active substance of which is propylene glycol, were tested and applied with little success, partial2003

200 083 fatty acid esters of glycerol, condensation products of ethylene oxide with fatty acids polypropylene glycols now especially polysiloxane esters. Application of the latter substances is carried out either in ICO% form, where, due to high hydrophobicity, their coalescence occurs on the surface of the liquid medium, which significantly reduces their effects, or is carried out in admixture with mineral or vegetable oil as carrier, The desired test results do not reach the required high antifoam effects and result in subsequent technological problems during application.

It has now been found that these drawbacks can be eliminated and high antifoaming effects can be achieved by the composition according to the invention consisting of an emulsion mixture of 0.4 to 1.0 wt. parts of demethylpolysiloxane,

0.3 to 1.5 wt. parts of refined wool grease; and

0.3 to 1.5 wt. % by weight of the partial esters of glycerol with fatty acids of the series C 1 to C 8, in which cis-13-docosenoic acid is predominant, and a content of 90 to 96% by weight; % monoglycerides of fatty acids dispersed in 60 to 95, preferably 85 wt. carriers from the group of triglycerides of fatty acids of the C ^ g and i GI series with an iodine number of 80 to 130 »fatty acids of the C ^ g series of an iodine number of 8 ^U si 130 fatty acid adducts of the C ^ g ai series with an iodine number of 60 to 130 e 3 to 8 moles of ethylene oxide adducts of fatty acids of C ^ g ai C ₂₄ poisonous numeral 60 a 130 5-10 moles propylenoxldu, sduktů fatty acids of C ^ g-C ₂ * by an iodine number from 60 to 130 with 3 to 8 moles of ethylene oxide and 5-10 moles of propylene oxide, fatty alkohslů series _C-2 to Ο ^ θ, adducts of fatty alcohols series C ^ ₂ and with 3-8 moles of ethylene oxide adducts of fatty alcohols of the series _{C12 IE} and 5-10 moles of propylene oxide, adducts fatty alcohols of the series C ₂ to C 6 g with 3 to 8 moles of ethylene oxide and with 5 to 10 moles of propylene oxide and medical vaseline oil or mixtures thereof

The high antifoam effect of the composition according to the invention was ensured not by the discovery of new properties of the present emulsion composition. These properties are, in particular, the ability to form a perfect dispersion in said carriers and, in this dispersed form, the ability to cause chain deactivation of the foam and due to high surface scattering which spontaneously occupies the entire surface of the fermenter liquid medium.

By utilizing the present invention, high antifoaming effect, very good organoloptical properties of the fermentation products and simplification of the process technology in the product separation and washing phase can be achieved.

200 083

Example 1

To 50 g of refined fat vlního was added 25 g of a partial ester of glycerol and matt kyaelinami Series C ^ g • ^from No. ₂₄ and containing 90% by weight. monoglycerides of fatty acids, of which 40 wt. consisted of cia-13-docoaenoic acid, and 50 g of dimethylpolyailoxane. The mixture was heated to 80 ° C and dispersed, while stirring, into 875% oleic acid adduct 80 and 6 small ethylene oxide. Passing through the emulsifier, while cooling to 20 ° C, formed a stable emulsion.

Example 2

Ks 100 g of refined fat vlního added 100 g ae partial oateru glycorolu and matt acids series C ^ g to _C24 and containing 95% by weight. monoglycerides of the fatty acids, of which 45 wt. consisted of cis-13-docesenevic acid, and 100 g of ethyl polyailexane.

The mixture was heated to 60 ° C and dispersed with stirring to mix 500 g of oleic acid adduct of iodine number 80 and 6 moles of ethylene oxide and 7 mole of propylene oxide and 200 g of fatty acid kyaolins of rapeseed oil of iodine number 100. adjusted to a stable dispersion.

Example 3

To 150 g of refined worm fat aa was added 100 g of a partial ester of glycorol and fatty acids of the C18 to _C24 series with a content of 92% by weight. moneglyoerlds fatty acids, of which 48% touch. consisted of cia-13-docoaeaic acid and 150 g of dimethylpolyailoxane. The vanic acid was formulated into an emulsion which was dispersed in a mixture of 400 g of methyl methanoic acid adduct of iodine numbered 100 and 3 moles of ethylene oxide and 200 g of alejic acid of iodine number 80. The mixture was prepared and dispersed.

Example 4

To 75 g of refined wool fat was added 50 g of partial glycerol ester and fatty acids. % _C24 to _C24 with 93 wt. fatty acid monoglycerides, of which 43% were cia-13-docosenoic acid and 50 g dimethylpolyailoxane. At the same time, it prepared an emulsion to which 580 grams of Iodine frosted rapeseed oil adduct of 100 and 5 moles of ethylene oxide and 5 moles of propylene oxide and 245 grams of sunflower oil iodine number 125 were added. dispersion.

Example 5

To 50 g of refined fat vlního was added 50 g of a partial ester of glycerol and serves any kyaelinami rows _L6 C to C ₂₄ β containing 92% by weight. monoglycerides of the fatty acids, of which 40 wt. consisted of cia-13-docosanoic acid and 50 g of dimethylpolysilexane. The mixture was heated to 60 ° C and dispersed with stirring into a mixture of 800 g dodecanol dodecanol with 4 moles of ethylene oxide and 6 moles of propylene oxide and 50 g of medical vaseline oil. Passing through the emulsifier, while cooling to 20 ° C, produced a stable emulsion.

Example of fermentation

200 083

A 30-liter glass laboratory fermenter with a 15-liter payload, equipped with a stirrer, air inlet and circulation cylinder, was charged with 12 kg of water, 2 kg of concentrated, sterilized molasses, containing 900 g of molasses, 1,063 g of water, 2 kg. superphosphate, 16 g ammonium hydrophosphate, 4 g ammonium sulfate and 15 g urea. 1 kg of inoculum prepared from 200 g of yeast paste having a dry weight of 23% by weight was added.

A temperature of 30 ° C, a pH of 4.5 and an air flow of 15 L per minute were maintained during 6 h of culture.

During the fermentation test to determine the antifoam efficiency, the antifoam was metered into the yeast medium automatically from a burette whose pressure aelenoid valve received an impulse to open it by means of a level electrode when the level of the emerging foam reached the electrode.

In the yeast fermentation process, β, 02 g of antifoam according to Example 4 in comparison and 23.51 g of oleic acid, which were consumed under the same model testing conditions, were consumed per kg of biomass produced.

The evaluation of the antifoaming effect of the fermentation agent according to the invention is expressed by the antifoam coefficient of the antifoam, which is the ratio by weight of pure oleic acid and the antifoam to be tested per 1 kg of biomass.

Type of defoamer * 1 x «* 8 * 9

Defoaming efficiency coefficient

1.00

0.33

1.86

1.47

2.10

2.50

2.00

2.93

1.95

Legend <

Xj - oleic acid of 80,

Xg - a mixture of vegetable fatty acids Cjg to 6 -4 ⁰ I.V. 115 neutral oils and vegetable oils medlclálního vesellnového,

Χθ - adduct of fatty acids C 8 to C 8 with an iodine number of 120 with 6 moles of ethylene oxide and 7 moles of propylene oxide,

X4-C8 to C8 fatty acid adduct 120 with 6 moles of ethylene oxide and 7 moles of propylene oxide in admixture with oleic acid 80 of iodine number 80,

Χθ - anti-foaming agent according to example 1,

Χθ - defoamer according to example 2,

Χγ- defoamer according to example 3,

Χθ - defoamer according to example 4,

Χθ - defoamer according to example 5.

200 003

Claims (1)

SUBJECT OF THE INVENTION Antifoaming agent for fermentation processes, characterized in that it is an emulsion mixture 0.4 to 1.0 wt. part of dimethylpolysiloxane, 0.3 to 1.5 wt. % of refined wool grease; and 0.3 to 1.5 wt. % of the partial esters of glycerol with fatty acids of the series C18 to 24%, in which preferably cis-13-docosenoic acid, with a content of 90 to 96 wt. % of monoglyoerides of fatty acids dispersed in 60 to 95, preferably 85 wt. carriers from the group of triglycerides of fatty acids of the series C ^g to Cg ^ having an iodine number of 80 to 130, fatty acids of the series C ^g to Cg ^ having an iodine number of 80 to 130, fatty adducts of the series C řadyg to Cg ^ having an iodine number of 80 up to 13θ with 3 to 8 moles of ethylene oxide, fatty acid adducts of the range C 8 to C 8 with an iodine number of 80 to 130 with 5 to 10 moles of prepylene oxide, fatty acid adducts of the series C ₁ to C 8 with an iodine number of 80 to 130 s 3 to 8 moles of ethylene oxide and 5 to 10 moles of propylene oxide, fatty alcohols of C ^g to ΟΟ řady, fatty alcohol adducts of C ^g to ΟΟ with 3-8 moles of ethylene oxide, fatty alcohol adducts of C ^g to ΟΟ θ 5 to 10 moles of propylene oxide, fatty alcohol adducts of the C 8 to 10 moles range with 3 to 8 moles of ethylene oxide and 5 to 10 moles of propylene oxide and media vaseline oil or mixtures thereof.