CS199764B1 - Pad for sampling material for microbiological investigation - Google Patents

Description

The invention aa relates to swabs for the collection of material for microbiological examination, in which aa solves a higher degree of survival of microorganisms using polyurethane foam.

They are currently using swabs made of cellulose wool wrapped on a wooden block or on a rod and plastic to collect material for microbiological examination. The cotton swab portion of aa uses non-impregnated or impregnated media to stabilize the microorganisms on the swab during transport to the microbiological laboratory. The manufacture of such tampons is generally manual and the mechanism of production of such tampons is extensive. In manual production, it is necessary to wind the dose of cotton wool onto a wooden stick so that the cotton wool is well fixed, properly formed on the rod and does not unwind. These relatively complicated operations upon which the mechanical quality of the tampons depend are difficult to mechanize. In the manufacture of cotton swabs it is necessary to impregnate them further with a stabilizing medium, since the cotton wool has a toxic effect on medically important microorganisms.

This requires further laborious and tedious technological operations on the cotton swabs, namely the soaking of the cotton swabs on the rod has been designed with complicated dispensers, which they use in the production of cotton wool. (DS Pat. 3,090,080 and 3,554,512). The quality of these tampons when used for the collection of microbiological material is negatively affected by the toxic effect of cotton wool on microorganisms. Other ma199 764 are also used to make tampons

199,794 terriers than cotton (US Pat. 3,978,855, 3,809,088 and DOS 27,092,152), but are tampons intended for personal hygiene purposes, reap. ehirurgiu.

The drawbacks of the prior art swabs for collecting microbiological material are overcome by the present invention. Its essence is that instead of the cotton part of the tampons hitherto used, a polyurethane pan is attached at the end of the rod. Due to the inertness of the polyurethane foam to the microorganisms, such tampons do not have a toxic or toxic effect. inhibitory effect on microorganisms and therefore need not be impregnated in the stabilizing medium. However, especially the use of such tampons does not cure the possibility of their impregnation. Such a sterile polyurethane swab is enclosed in a package that guarantees sterile transport from the site of collection to the microbiological laboratory.

The tampons of the present invention have a higher stabilizing property for the transported microorganisms than both conventional cotton swabs and cotton swabs (impregnated with stabilizing medium, both in terms of catching low contamination and in terms of the survival time of the microorganisms. Used for testing the quality of tampons, for 48 hours, on the tampon it is necessary to contaminate the cotton swabs with this microorganism in an amount of 10 microns, the cotton swabs impregnated with thioglycol stabilizing medium with 10 microns and in the polyurethane swabs Fresh field and laboratory strains of beta streptococcal inoculated on polyurethane swabs in an amount of 10 ^ to 10 ² * · survived on these swabs for 10 days. The properties of the polyurethane tampons were also tested in relation to the most delicate medically important microorganisms in relation to the strain N. gonoreae. The inert, non-toxic effect of polyurethane tampons on these microorganisms has been confirmed. Even in practical use, the polyurethane tampons of the present invention have identified more delicate microorganisms that have not been able to transport for the time being on the tampons. It has been shown that polyurethane swabs do not have a toxic effect on tissue cultures, e.g. to Hela lines, HEp-2,

L-132 and dioploid cells. These swabs have no toxic or inhibitory effect on adenoviruses, ES virus, also on mycoplasma 1S pneumoniae, li orale, M hominis.

In addition to the above described microbiological superiority of the polyurethane swabs according to the invention, the suction effect of the polyurethane foam is positively evaluated for the polyurethane swabs, which positively affects the amount of sample taken and consequently the higher inoculum density.

The use of polyurethane foam for the production of material swabs for microbiological examination allows for a simplified process of their production. The theology of polyurethane foam swabs is simplified, as opposed to cotton swab technology, for the following operations: Obtaining a piece of polyurethane foam of a suitable shape and its fixation on a stick. Epoxy polyurethane foam, cone, prism, resp. tears can be obtained eg. by mechanical die-cutting, or by heat-cutting a resistive wire, being

199 794 suitable to burn a hole in the thus obtained piece of polyurethane foam to fit a stick ·

A rod of e.g. made of plastic by gluing or melting. These technological operations are easier to mechanize than cotton swab technology, including impregnation, so that the polyurethane swab machine may be envisaged; · The original properties of conventional tampons are preserved for polyurethane tampons.

Claims (2)

1. Polyurethane foam of polyester or polyether type, of cylindrical, conical, prism, tear-like shape is fixed on a plastic rod, eg thermoplastic, by sliding it into the hole in the polyurethane foam, gluing it with a glue inert to the microorganisms or fusing it to the adjusted rod. For the purpose of producing polyurethane tampons, e.g. polyurethane foam prepared by the addition reaction of polyisocyanates and compounds rich in hydroxyl groups in the presence of catalysts having the following physical parameters: Bulk density 20 - 5% kg / m,, specific strain at 40% deformation height 0.002 - 0.004 - MPa, tensile strength 0, 07 - 0,14 MPa, elongation 120 200%, permanent deformation max. 4.0 - 8.5% »Such a tampon is hermetically sealed in a sterile state in the package, which allows careful hermoetic sealing of the package with the sampled tampon, by sealing or by sealing the opening of the package. Suitable wrapping for tampons are thermo-paste plastic wrap - foil eg. of polyethylene, polyamide or the like, or the package consists of a tub of tough polystron closed by a plastic sheet by gluing to the edge of the tub. 2. Carrying out as in step 1, except that the tampon's polyrethane foam is impregnated with microbial stabilizing medium prior to sealing into the package by immersing the polyurethane portion of the tampon in a solution of the medium and drying by drying. pebdmbt of the invention The swab for microbiological examination consists of a rod on which the mass is attached, in particular in the form of a cylinder, a cone, a prism or a tear, which mass is optionally impregnated with a medium stabilizing the transported microorganisms. according to claim 1, characterized in that the mass fixed on the rod consists of polyurethane foam, in particular of the polyester or polyester type.