CO2020014388A2 - Sistema y método para clarificar una solución de recolección de cultivo celular - Google Patents

Sistema y método para clarificar una solución de recolección de cultivo celular

Info

Publication number
CO2020014388A2
CO2020014388A2 CONC2020/0014388A CO2020014388A CO2020014388A2 CO 2020014388 A2 CO2020014388 A2 CO 2020014388A2 CO 2020014388 A CO2020014388 A CO 2020014388A CO 2020014388 A2 CO2020014388 A2 CO 2020014388A2
Authority
CO
Colombia
Prior art keywords
filter
backwash
clarifying
cell culture
culture collection
Prior art date
Application number
CONC2020/0014388A
Other languages
English (en)
Inventor
José Castillo
Vasily Medvedev
Tiago Albano
Original Assignee
Exothera Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exothera Sa filed Critical Exothera Sa
Publication of CO2020014388A2 publication Critical patent/CO2020014388A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/14—Pressurized fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
    • B01D2239/04—Additives and treatments of the filtering material
    • B01D2239/0414—Surface modifiers, e.g. comprising ion exchange groups
    • B01D2239/0428—Rendering the filter material hydrophobic

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)

Abstract

Un sistema para clarificar una solución de recolección de cultivo celular, que incluye moléculas objetivo y un medio de filtrado dinámico. Un recipiente de filtración incluye un recubrimiento flexible y al menos un filtro que tiene una superficie sobre la cual se acumula el medio de filtrado dinámico en una torta. La torta y el filtro, durante la operación de filtrado, permiten el paso de un filtrado que incluye moléculas objetivo mientras previenen que pasen materiales sólidos no deseados. Una fuente de lavado a contracorriente incluye al menos un fluido de lavado a contracorriente y está conectada fluidamente con el recipiente de filtración mediante el al menos un filtro. La fuente de lavado a contracorriente, durante la operación de lavado a contracorriente, está adaptada para suministrar fluido de lavado a contracorriente a través del al menos un filtro para remover la torta formada en el filtro. También se divulgan sistemas y métodos relacionados.
CONC2020/0014388A 2018-05-11 2020-11-19 Sistema y método para clarificar una solución de recolección de cultivo celular CO2020014388A2 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862670220P 2018-05-11 2018-05-11
US201962827009P 2019-03-30 2019-03-30
PCT/EP2019/062227 WO2019215344A2 (en) 2018-05-11 2019-05-13 System and method for clarifying a cell culture harvest solution

Publications (1)

Publication Number Publication Date
CO2020014388A2 true CO2020014388A2 (es) 2021-03-08

Family

ID=66647366

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2020/0014388A CO2020014388A2 (es) 2018-05-11 2020-11-19 Sistema y método para clarificar una solución de recolección de cultivo celular

Country Status (7)

Country Link
US (1) US20210189454A1 (es)
EP (1) EP3790983B1 (es)
CO (1) CO2020014388A2 (es)
EC (1) ECSP20078797A (es)
ES (1) ES2957624T3 (es)
PE (1) PE20210741A1 (es)
WO (1) WO2019215344A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2021403434A1 (en) * 2020-12-14 2023-06-22 Chreto Aps A method and a system for filtering a suspension

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2029722T3 (da) * 2006-05-22 2020-01-02 Biovest Int Inc Fremgangsmåde og system til celleproduktion
EP2470638B1 (en) * 2009-08-26 2019-10-23 GE Healthcare Bio-Sciences Corp. Continuous recovery harvest bag
US9376655B2 (en) * 2011-09-29 2016-06-28 Life Technologies Corporation Filter systems for separating microcarriers from cell culture solutions
DE102011119816B4 (de) * 2011-12-01 2015-08-27 Sartorius Stedim Biotech Gmbh System und Verfahren zur Zellabtrennung
GB201508752D0 (en) * 2015-05-21 2015-07-01 Mason Christopher And Veraitch Farlan S Cell culture device, system and methods of use thereof
GB201617713D0 (en) * 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
CN115232745A (zh) * 2016-12-01 2022-10-25 生命科技股份有限公司 微载体过滤袋总成和使用方法
US20210140858A1 (en) * 2017-02-23 2021-05-13 Otsuka Pharmaceutical Factory, Inc. Intestinal flora extraction vessel
BE1025089B1 (fr) * 2017-03-30 2018-10-29 Univercells Sa Clarification de culture cellulaire
US11485947B2 (en) * 2017-05-31 2022-11-01 Global Life Sciences Solutions Usa Llc Hollow fiber filter arrangement

Also Published As

Publication number Publication date
ES2957624T3 (es) 2024-01-23
PE20210741A1 (es) 2021-04-19
EP3790983A2 (en) 2021-03-17
EP3790983B1 (en) 2023-07-05
EP3790983C0 (en) 2023-07-05
WO2019215344A3 (en) 2019-12-26
WO2019215344A2 (en) 2019-11-14
US20210189454A1 (en) 2021-06-24
ECSP20078797A (es) 2021-04-29

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