CN86100081A - The wheat root growth determination method of the basic element of cell division - Google Patents
The wheat root growth determination method of the basic element of cell division Download PDFInfo
- Publication number
- CN86100081A CN86100081A CN86100081.1A CN86100081A CN86100081A CN 86100081 A CN86100081 A CN 86100081A CN 86100081 A CN86100081 A CN 86100081A CN 86100081 A CN86100081 A CN 86100081A
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- cell division
- basic element
- wheat
- concentration
- wheat root
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Abstract
The present invention is a kind of biologicall test new method of the basic element of cell division.It with the wheat of sprouting as effective object.Wheat under the basic element of cell division effect of high concentration, the length of the root that it grows is just short more; The concentration of effect is low more, and the length that wheat sends out roots is just long more.
Come compared with methods such as former radish cotyledon, cucumber cotyledons, amaranthus caudatus amaranth haematochrome are synthetic, have that equipment is simple, minute short and the advantage of favorable reproducibility.
Description
The invention belongs to the scope of plant growth regulator assay method.
Measure the concentration of the basic element of cell division in the past, generally adopted methods such as radish cotyledon, cucumber cotyledons, amaranthus caudatus amaranth red pigment synthesize both at home and abroad.Measure with these common methods, the time is minimum to be 3 days; Also need instrument and equipments such as incubator, spectrophotometer; And the mensuration program is loaded down with trivial details, poor reproducibility.Especially measure the content of the basic element of cell division in the actinomyces 5406 (Streptomyces jingyangensis nsp.), lack effective method.Therefore a kind of simple and effective method is studied in an urgent demand.
Purpose of the present invention: be that a kind of minute of searching is short, method is simple, the new method of favorable reproducibility, especially the product for 5406 factories provides the effective method of inspection.
When using this method,, then the wheat of having sprouted is put into double dish, and inject test solution, it is put into incubator cultivate at first with wheat sterilization, seed soaking, vernalization, last, measure wheat root total length.The wheat root total length of wheat root total length and typical curve or standard solution is contrasted.In 0.5PPM to 200PPM scope, concentration is high more, and wheat root total length is just short more; Concentration is low more, and wheat root total length is just long more.Therefore, just know the concentration of the basic element of cell division of test solution.
Use said method, the time is 24 to 48 hours.As long as possess simple utensil such as incubator, double dish.And the result of test is more consistent.Therefore have that minute is short, method is simple, the advantage of favorable reproducibility.Can be the basic element of cell division, the particularly examination and test of products of 5406 factories provides a kind of simple and effective bioassay method.
The optimum implementation of foregoing invention is exemplified below:
" Qian Jiangyi number " wheat is put into 0.1% mercuric chloride solution sterilization 5 minutes, and the water flushing adds suitable quantity of water and puts into 25 ℃ of incubators seed soaking 24 hours for several times again, outwells water then, puts into 25 ℃ of incubator vernalization 24 hours (till showing money or valuables one carries unintentionally).Again the wheat of having sprouted is put into clean double dish (30 to 50 in every ware), then inject the test solution that is diluted into various variable concentrations.The incubator that puts it into 25 ℃ was cultivated 30 hours.At last, survey every ware Nei Maigen total length, and compare with the wheat root total length of standard solution; Or compare with typical curve.In 0.5PPM to 200PPM scope, the concentration of the basic element of cell division is high more, and wheat root total length is just short more; Concentration is low more, and wheat root total length is just long more.Therefore, just know the concentration of the basic element of cell division in the test solution.
Claims (3)
1, a kind of cytokine activity that is used for especially for the biologicall test new method that actinomyces 5406 (Streptomycesjingyangensisnsp.) is tired, is characterized in that selecting for use the effective object of wheat as the tested basic element of cell division of sprouting.
2, new method as claimed in claim 1 is characterized in that the root length of wheat becomes the anti-corresponding relation that increases with the concentration of the basic element of cell division that is applied.
3, as claim 1,2 described new methods, it is characterized in that: effective in cytokinin concentration is 0.5PPM to 200PPM scope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN86100081.1A CN1005604B (en) | 1986-01-04 | 1986-01-04 | Organism method using the root length of wheat grain for measuring the effect of cytokinin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN86100081.1A CN1005604B (en) | 1986-01-04 | 1986-01-04 | Organism method using the root length of wheat grain for measuring the effect of cytokinin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN86100081A true CN86100081A (en) | 1986-09-10 |
| CN1005604B CN1005604B (en) | 1989-11-01 |
Family
ID=4800879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN86100081.1A Expired CN1005604B (en) | 1986-01-04 | 1986-01-04 | Organism method using the root length of wheat grain for measuring the effect of cytokinin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1005604B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109373853A (en) * | 2018-10-24 | 2019-02-22 | 杭州师范大学 | Water culture and root measurement device and application method |
-
1986
- 1986-01-04 CN CN86100081.1A patent/CN1005604B/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109373853A (en) * | 2018-10-24 | 2019-02-22 | 杭州师范大学 | Water culture and root measurement device and application method |
| CN109373853B (en) * | 2018-10-24 | 2024-03-01 | 杭州师范大学 | Plant hydroponic and root length measuring device and using method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1005604B (en) | 1989-11-01 |
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