CN85102491A - Technique of fish cell clone culture - Google Patents
Technique of fish cell clone culture Download PDFInfo
- Publication number
- CN85102491A CN85102491A CN198585102491A CN85102491A CN85102491A CN 85102491 A CN85102491 A CN 85102491A CN 198585102491 A CN198585102491 A CN 198585102491A CN 85102491 A CN85102491 A CN 85102491A CN 85102491 A CN85102491 A CN 85102491A
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- China
- Prior art keywords
- cell
- fish
- clone
- technique
- fish cell
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Fish cell clone culture technique belongs to A01K, fish, fish cell engineering.Clone cultivation with unicellular micrurgy isolated mononuclear cell, and make the feeder cells of clone's cultured cell with tilapia kidney primary cell, through duplicating, increasing, can obtain a large amount of cell clones in a short time, cloning efficiency is reached more than 50%, reach as high as 92%.This technology can be widely used in cellular morphology, cytogenetics, cell one virus relation and the segregation mutant etc. of researching fish, and will promote the development of fish biotechnology and monoclonal antibody technique.
Description
The invention belongs to A01K, fish, fish cell engineering.
The present invention is beyond example still at home and abroad, does not have the cloning of seeing fish cell to cultivate document retrieval as seen so far:
The river rattan U.S. 1978 is lost 32(7 for a long time): 64-71.
The objective of the invention is to cultivate the fish cell clone, the cellular morphology of researching fish, the biochemical characteristic of cell, cytogenetics, cell one virus relation, mutant separate, and promote the development of fish cell engineering and monoclonal antibody technique.
Fish cell clone culture technique comprises three steps:
1. preparation feeder cells: get the nephridial tissue of Tilapia mossambica, after cold digestion,, cultivate former generation cell monolayer, make feeder cells and use by 1,000,000 cells/ml inoculations.
2. separating clone: fish cell that will be to be cloned goes down to posterity, select well-grown cell that form is neat, color and luster bright, the kytoplasm endoparticle is few, with the cell micrurgy individual cells immigration is added with in 0.1 milliliter of feeder cells, the 96 hole microtest plates of (containing ten thousand cells of 1.5-2.0 approximately) in advance, add a cover, the adhesive tape sealing, 27 ℃ of incubators are cultivated.
3. duplicate cultivation: when cell in 96 hole microtest plates of inoculation when growing to more than half hole, can be digested to cell suspension after, utilize and duplicate culture technique, shift amplification, can obtain a large amount of cell clones.
Present technique is more than 50% to the cloning efficiency of fish cell, and high energy reaches 92%, and separates the fish cell clone with the cells of mamma animals cloning process, and its cloning efficiency is 30%.Therefore, present technique has advantages such as cloning efficiency is higher, and incubation time is shorter, does not need to handle feeder cells through X ray, and is easy to use, can obtain a large amount of cell clones in a short time.
Separating individual cells to be cloned with the cell micrurgy, cultivate cell monolayer with the Tilapia mossambica kidney and make feeder cells, and duplicate that cultivating combines shifts the technology of amplification, is the best way that realizes this patent.
Claims (3)
1, fish cell clone culture technique is characterized in that fish cell to be cloned makes feeder cells with Tilapia mossambica kidney cell monolayer of former generation.
2, fish cell according to claim 1 clone culture technique is characterized in that choosing that form is neat, color and luster bright, the kytoplasm endoparticle is few, and well-grown individual cells is cloned cultivation.
3, cell clone according to claim 2 is characterized in that separating clone and duplicates culture technique and combine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN198585102491A CN85102491A (en) | 1985-04-01 | 1985-04-01 | Technique of fish cell clone culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN198585102491A CN85102491A (en) | 1985-04-01 | 1985-04-01 | Technique of fish cell clone culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN85102491A true CN85102491A (en) | 1986-09-24 |
Family
ID=4792553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN198585102491A Pending CN85102491A (en) | 1985-04-01 | 1985-04-01 | Technique of fish cell clone culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN85102491A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321177C (en) * | 2005-09-05 | 2007-06-13 | 中国水产科学研究院黄海水产研究所 | Fish embryo cell separation and culturing method |
-
1985
- 1985-04-01 CN CN198585102491A patent/CN85102491A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321177C (en) * | 2005-09-05 | 2007-06-13 | 中国水产科学研究院黄海水产研究所 | Fish embryo cell separation and culturing method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |