CN2926237Y

CN2926237Y - Two-purpose inoculation opening of fermenter

Info

Publication number: CN2926237Y
Application number: CN 200620023500
Authority: CN
Inventors: 徐亲民
Original assignee: Hebei University of Science and Technology
Current assignee: Hebei University of Science and Technology
Priority date: 2006-02-08
Filing date: 2006-02-08
Publication date: 2007-07-25
Anticipated expiration: 2016-02-08

Abstract

The utility model relates to a fermenter convertible inoculation opening, which comprises an inoculation oral siphon and an inoculation oral cap being threadedly connected with the inoculation oral siphon. An O-shape loop stationary core sleeved with an O-shape rubber seal ring is positioned between the inoculation oral cap and the inoculation oral siphon. The utility model is characterized in that the O-shape loop stationary core is a hollow tube having a boss on the top of the tube, in the superior hole is provided a Schering bottle rubber stopper, and on the inoculation oral siphon cap is positioned the oral cap hole, the aperture of which is less than the external diameter of the Schering bottle rubber stopper. The utility model provides an inoculation opening which can be used both in the fire ring inoculation and in the pressure difference inoculation, thereby bringing a great deal of convenience to the routine operation of the fermenter convertible inoculation opening in particular to the experimental operation.

Description

The dual-purpose inoculation mouth of fermentor tank

Technical field

The utility model relates to the dual-purpose inoculation mouth of a kind of fermentor tank.

Technical background

As the fermentor tank of first class seed pot, need an inoculation mouth, so that shake-flask seed, rice spore or spore suspension are inoculated in the jar.To bring assorted bacterium in order preventing to inoculate into, can to adopt inoculation of pyrosphere impouring method and pressure difference injection method inoculating two kinds operating method usually.As shown in Figure 1, the former uses bigger inoculation mouth, adds a pyrosphere during inoculation around inoculation mouthful, and tank pressure is put to zero gauge pressure, opens inoculation port cap rapidly, under the flame protection with in seed liquor or the rice spore grain impouring jar; As shown in Figure 2, the latter uses less inoculation mouth, a cillin bottle rubber plug just in time can be filled in the ingress, on nut compressing with holes is arranged, need not open inoculation mouthful nut during inoculation, not need pressure release yet, only need the needle tubing on the special seed bottle is penetrated in the rubber plug via screw cap hole, by lifting fermentor tank internal pressure, seed liquor (generally being spore suspension) is pressed in the jar.The advantage of these two kinds inoculation mouths is loosen nut when sterilization, and part steam is leaked, and reaches the purpose of abundant sterilization.They are applicable to different occasions, should look the difference of inoculation seed form and select for use.Hyphomycetic shake-flask seed and rice spore grain must adopt the inoculation of pyrosphere method; And unicellular seed liquor and spore suspension, both available pyrosphere method inoculation, also available pressure difference method inoculation.Because differing greatly in the design, a seeding tank is generally only installed a kind of inoculation mouthful, when needs are changed inoculation method, then needs to install additional another inoculation mouthful, thus to regular job particularly experimental implementation make troubles.

Summary of the invention

Technical problem to be solved in the utility model provides an a kind of inoculation mouth both can carry out the pyrosphere inoculation, also can carry out the pressure difference inoculation, thereby was very easy to the particularly dual-purpose inoculation mouth of fermentor tank of experimental implementation of regular job.

The utility model adopts following technical scheme:

The utility model comprises inoculation port tube and adopts the inoculation port cap that is threaded with inoculation port tube, between inoculation port cap and inoculation port tube, add the O type circle fixed core that is with O type rubber seal, it is characterized in that O type circle fixed core is the hollow tube that there is boss a upper end, its endotheca of upper hole is equipped with the cillin bottle rubber plug, and inoculation port cap is provided with the port-cap hole of aperture less than cillin bottle rubber plug external diameter.

The utility model is corresponding with O type rubber seal on the tube wall of inoculation port cap to be provided with two screws, is provided with the fixedly inoculation port cap standing bolt of O type rubber seal in screw.

The utility model positively effect is as follows: the utility model provides an a kind of inoculation mouth both can carry out the pyrosphere inoculation, also can carry out the pressure difference inoculation, thereby is very easy to the particularly dual-purpose inoculation mouth of fermentor tank of experimental implementation of regular job.

Description of drawings

Accompanying drawing 1 is traditional pyrosphere inoculation mouth

Accompanying drawing 2 is pressure difference inoculation mouth

Accompanying drawing 3 is the wiring layout of the dual-purpose inoculation mouth of the utility model

Accompanying drawing 4 is the utility model bolt arrangement synoptic diagram

Accompanying drawing 5 is the left view of the utility model accompanying drawing 4

Accompanying drawing 6 is the utility model inoculation port cap handle arrangement synoptic diagram

Accompanying drawing 7 is the utility model accompanying drawing 6 left views

Accompanying drawing 8 is the structural representation of the dual-purpose inoculation mouth of the utility model

Steam flow figure when accompanying drawing 9 is the sterilization of the dual-purpose inoculation mouth of the utility model

In the accompanying drawings: 1 inoculation port cap, 2 inoculation port cap bolts, 3 cillin bottle rubber plugs, 4 O type circle fixed core, 5 O type rubber seal, 6 inoculation port tubes, 7 mouthfuls of cap handles, 8 port-cap holes, 9 screws, 10 fermentor tank top covers.

Embodiment

Each integral part as shown in Figure 3.Wherein inoculation port tube is vertically welded on the corresponding aperture of fermentor tank top cover 10 as shown in Figure 1, and the assembling of remaining part and inoculation port tube as shown in Figure 8.The material that all parts use should be consistent with the fermentor tank material, but stainless steel preferably, so that durable in use and keep certain surface flatness.Its size can be determined according to the size of cillin bottle rubber plug 3 that uses and O shape rubber seal 5 and the rigidity and the withstand voltage properties of material.The reference dimension of major parts is:

Inoculation port tube 6 internal diameter Φ 20～32mm

Inoculation port tube 6 outside screw M25～40mm

Inoculation port cap 1 external diameter Φ 32～50mm

Inoculation port cap 1 internal thread M25～40mm

Switch inoculation port cap handle 7 is unsuitable long, and left rotation and right rotation freely and non-scald on hand degree of being during with inoculation.

Inoculation port tube 6 outside screws match with inoculation port cap 1 internal thread, cillin bottle rubber plug 3 is wringing fit (wherein O shape rubber seal 5 just in time can be inserted in the groove of O shape circle fixed core 4) with O shape circle fixed core 4 internal diameters and O shape rubber seal 5 with O shape circle fixed core 4 constriction external diameters, and O shape rubber seal 5 external diameters and inoculation port cap 1 inwall, O shape circle fixed core 4 protuberance external diameters and inoculation port cap 1 inwall and O shape are enclosed the slit that should have between fixed core 4 constriction external diameters and inoculation port tube 6 inwalls about 1mm as shown in Figure 8.

Disinfecting action method and step are as follows:

1, opens inoculation port cap 1, unclamp inoculation port cap standing bolt 2, get lower O-shape ring fixed core 4, change O shape rubber seal 5 and cillin bottle rubber plug 3;

2, the O shape circle fixed core 4 that will have O shape rubber seal 5 and a cillin bottle rubber plug 3 is put inoculation mouthful into, covers inoculation port cap 1, suitably unclamps the 1-2 circle after screw thread is screwed to the end;

3, fermentor tank begins sterilization, looks the steam situation that leaks and adjust the degree that inoculation port cap unclamps in sterilization process, and the steam flow during sterilization as shown in Figure 9;

4, sterilization finishes, and tightens inoculation port cap 1, blocks hole 8 on the inoculation port cap with alcohol swab, with screwdriver the inoculation port cap standing bolt 2 in the inoculation port cap 1 both sides screw 9 is tightened again;

The effect of inoculation port cap standing bolt 2 is that O shape rubber seal 5 and inoculation port cap 1 are fixed together, from cap, come off when preventing to adopt the inoculation of pyrosphere inoculation method to open inoculation port cap, therefore the sterilization back only need be tightened a little, and it is firmly undue to must guard against, with antisitic defect O-ring seals and fixed core thereof.

Inoculation working method and step are as follows:

1, pyrosphere method inoculation: around the inoculation mouth, put pyrosphere, the closes compression air door also stops stirring, open the drain tap pressure release, igniting in the time of pressure release, treat that pressure puts near closing drain tap behind zero pound, one people hands inoculation port cap handle 7, slowly open inoculation port cap 1 and continue pressure release, unload inoculation port cap 1 more rapidly, be suspended from the flame top to zero pound, expose the inoculation mouth, another people above pyrosphere in the impouring inoculation mouthful, covers seed liquor or spore grain rapidly inoculation port cap 1 then and tightens, and successively opens pressurized air valve and drain tap, the fermentor tank internal pressure is adjusted to desirable value and open stirs, remove or extinguish pyrosphere;

2, pressure difference method inoculation: this method is without pyrosphere and open inoculation port cap 1, also need not stop ventilation and stirring, only needs one man operation.Operation steps is earlier the cotton in the inoculation port cap hole 8 to be taken out, the special inoculation bottle needle tubing through sterilization is pricked saturating cillin bottle rubber plug 3 via inoculation port cap hole 8, improve the fermentor tank internal gas pressure to 0.08MPa, at this moment visible bubble in bubble is overflow to inoculation bottle, treat that bubble stops to overflow into, illustrate that the inoculation bottle internal pressure has reached and fermentor tank internal gas pressure equilibrated 0.08MPa, again the fermentor tank internal gas pressure is reduced to 0.02MPa, seed liquor or spore suspension in the inoculation bottle begin to be pressed in the fermentor tank, boost so repeatedly, step-down can all be pressed into seed liquor in the inoculation bottle or spore suspension in the fermentor tank several times.

Must be noted that when carrying out the pressure difference inoculation pressure that does not make in the inoculation bottle surpasses 0.08MPa, in case the vial explosion is hurted sb.'s feelings.Be anti-contingency, Stainless Steel Wire or copper wire gauze should be covered in the inoculation bottle outside during inoculation.

Claims

1, the dual-purpose inoculation mouth of a kind of fermentor tank, it comprise inoculation port tube (6) and and inoculation port tube (6) adopt the inoculation port cap (1) be threaded, between inoculation port cap (1) and inoculation port tube (6), add the O type circle fixed core (4) that is with O type rubber seal (5), it is characterized in that O type circle fixed core (4) is the hollow tube that there is boss a upper end, its endotheca of upper hole is equipped with cillin bottle rubber plug (3), and inoculation port cap (1) is provided with the port-cap hole (8) of aperture less than cillin bottle rubber plug (3) external diameter.

2, the dual-purpose inoculation mouth of a kind of fermentor tank according to claim 1, it is characterized in that corresponding two screws (9) that are provided with on the tube wall of inoculation port cap (1), in screw (9), be provided with the fixedly inoculation port cap standing bolt (2) of O type rubber seal (5) with O type rubber seal (5).