CN2881620Y - Automatic analyser for antibiotic titre - Google Patents
Automatic analyser for antibiotic titre Download PDFInfo
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- CN2881620Y CN2881620Y CN 200520045092 CN200520045092U CN2881620Y CN 2881620 Y CN2881620 Y CN 2881620Y CN 200520045092 CN200520045092 CN 200520045092 CN 200520045092 U CN200520045092 U CN 200520045092U CN 2881620 Y CN2881620 Y CN 2881620Y
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- ccd
- power supply
- monitor board
- excitation power
- digital camera
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Abstract
The utility model relates to an antibiotics titer analyzer, which comprises a bacteriostatic ring measuring device and an external PC machine. The bacteriostatic ring measuring device is contained in a measuring casing, with a case power supply, a CCD monitoring panel, a keypress panel, an excitation power panel, a ultraviolet sterilamp, a CCD digital photography device, a back light source and a linear guide rail. A sample stand is inserted into the measuring casing from the insert port of the sample plate and placed on the linear guide rail. The CCD digital photography device and the back light source are above and below the sample stand respectively. The CCD monitoring panel is connected with the CCD digital photography device through the internal video line, with STC89C58 single chip machine on the monitoring panel controlling the photographing mode of the CCD digital photography device. The excitation power panel includes a D/A module and an excitation power module, among which the excitation power module transforms 12V direct current output from the back light source CCD monitoring panel into 70V alternating current, so as to provide power for the back light source. A video capture board is also equipped in the external PC machine and the video capture board is connected with the CCD monitoring panel. The utility model is applied to measure the diameter of the bacteriostasis plaque and automatically judge the results and print the report.
Description
Technical field
The utility model relates to a kind of analytical instrument, particularly the titer of antibodies analyser.
Background technology
Microbiotic is that application anti-inflammation drugs is very widely gone up in medical treatment, and for guaranteeing its quality, factory and medicine prison department all need by the state-promulgated pharmacopoeia regulation it to be carried out the strictness check.Antibiotic titration method is to utilize microbiotic to spread in the nutrient culture media that carries disease germs, and forms the inhibition zone that suppresses experimental strain.Under certain condition, the logarithm concentration dose is directly proportional with the inhibition zone area.As long as, just can extrapolate this antibiotic tiring so measure the area of inhibition zone.Therefore, the mensuration to the inhibition zone area is the key that titer of antibodies is measured.To the mensuration of inhibition zone, adopted vernier calipers by manual measurement more in the past, or on the ground glass through being projected to the band scale, again by artificial reading.Above-mentioned two kinds of methods are not only taken great energy time-consuming, and can introduce the error in subjectivity or the observation.In the hand dipping process, because human factor, result's objectivity can't guarantee that measurement result can't be preserved in addition, if the expired destruction of sample, the doctor more has no way of retrieving.In case generation medical tangle, hospital can't put to the proof.In recent years, the optical characteristics of the relative nutrient culture media of inhibition zone in the plate occurs utilizing, be designed to the camera head of projection-type light channel structure.Promptly penetrate plate by a branch of directional light, focus on through optical lens, imaging is on the target surface of camera.The problem that this structure exists is the requirement that the smooth finish of glass dish is had strictness.2, the contrast to the relative nutrient culture media of inhibition zone has strict requirement.Use this device, the lighter can increase measuring error, and weight person can influence the carrying out of surveying work.
Granted publication CN2274771Y discloses a kind of inhibition zone and has measured camera head automatically, include the georama of a diffuse reflection integration, along on the y direction at its center,, above this is open-topped, put pointolite respectively in open top with at bottom opening; Platform is shelved in the appropriate position of vertical center axis in this hollow ball, and heart district is embedded a light barrier therein; On this light barrier, put plate to be measured.
After adopting scanner scanning in addition aspect the inhibition zone measurement, in the input computer, adopt special analysis software to analyze at present.
Problems such as above-mentioned employing camera head and the subject matter that adopts scanner to measure the inhibition zone existence are that the efficient of measuring is not high, can not measure a plurality of inhibition zones simultaneously, and workload is bigger.
The utility model content
Technical problem to be solved in the utility model provides a kind of titer of antibodies analyser, to solve problems such as traditional inhibition zone efficiency of measurement is not high, workload is bigger.
The technical matters that the utility model solves is to realize by following technical scheme:
The titer of antibodies analyser comprises inhibition zone measurement mechanism and peripheral hardware PC, it is characterized in that; Described inhibition zone measurement mechanism comprises that one measures casing, in the front portion of measuring casing control panel is installed and has model insertion mouth, the back side of measuring casing be equipped with power switch, power interface and etc. voltage ground hold, in measuring casing, chassis power supply, CCD monitor board, keypad, excitation power source plate, ultraviolet disinfecting, CCD digital camera device, backlight and line slideway are installed, sample stage is inserted mouthful to insert from described model and is measured in the casing and be shelved on the line slideway, and CCD digital camera device and backlight lay respectively at the upper and lower of sample stage; External power supply is by the input end of power interface and power switch access chassis power supply, and chassis power supply provides 12V power supply to CCD monitor board, keypad, ultraviolet disinfecting, and the CCD monitor board provides 12V power supply to CCD digital camera device, excitation power source plate; The CCD monitor board is connected with the CCD digital camera device by the interior video line, by the screening-mode of the STC89C58 Single-chip Controlling CCD digital camera device on it; Keypad is connected with control panel, ultraviolet disinfecting, and is connected with the CCD monitor board by the control data line; The excitation power source plate comprises D/A module and excitation power source module, and the excitation power source module changes into the 70V alternating current with the 12V direct current of backlight CCD monitor board output, for backlight provides power supply; Also be provided with video frequency collection card in the peripheral hardware PC, video frequency collection card is connected with the RS232 interface with the AV terminal of CCD monitor board respectively with data line by the external video line.
Described control panel is provided with power light, CCD LCD display lamp, shooting termination button, takes startup button, sterilization pilot lamp, RESET reset button.
The utility model is equipped with the automatic Survey Software of inhibition zone in the peripheral hardware PC.
Workflow of the present utility model is:
1, start
Press the power switch opening power of analyser rear end panel, the power light of control panel can be lighted.Open PC and printer, the operation master routine.
2, system lands
Operation inhibition zone process of measurement enters user's login interface, and the input the user name and password is logined.
3, system's setting
By system the interface is set, takes delay adjustments.
4, nutrient culture media management
In system master interface, click " data base administration " menu, select " nutrient culture media management ", nutrient culture media is provided with.
5, the plain management of bacterial species
In system master interface, click " data base administration " menu, select " bacterial species management ", the bacterial species that system detected is provided with.
6, microbiotic management
In system master interface, click " data base administration " menu, select " microbiotic management ", the employed microbiotic of system is provided with.
7, double dish management
In system master interface, click " data base administration " menu, select " double dish management ", be provided with using double dish.
8, individual shooting
Click " individual shooting " button, enter individual and take the interface, flat board to be measured is put into sample stage, sample stage is pushed.Click " shooting " button, will take the picture of this flat board inhibition zone.
Fill in the sample number under this flat board in the sample information on the interface, bacterial classification and double dish type and other relevant informations of selecting this flat board to detect are clicked " warehouse-in " button, and system will analyze the inhibition zone information on this flat board automatically.If analyze successfully, system will provide relevant information.Repeat aforesaid operations, take all dull and stereotyped information.
9, take continuously
If have a large amount of same type double dish to read, can use continuous shoot function.
On the interface tool hurdle, click " taking continuously " button, perhaps in " workflow " menu, select " taking continuously " option, enter continuous shooting interface.
When taking continuously, choose check bacterial classification and double dish earlier, double dish to be checked is put into sample stage, push sample stage, press " take and the start " button on the control panel, at this moment the CCD LCD display lamp on the control panel will begin flicker, system will carry out the shooting and the analysis of inhibition zone picture automatically, after taking and analyzing successfully, the CCD LCD display lamp on the control panel will stop to flash, and will show shooting and the successful picture of analysis simultaneously in " pictures taken " tabulation at interface.
Take out sample stage, put into second double dish, press " take and the start " button on the control panel, proceed to take.If because the double dish quality problems, identification is success not, system will point out, call user's attention, and to the inhibition zone on this class double dish, the user needs manual being appraised and decided.
10, data audit
For each sample that system takes, the user must carry out the data audit.On toolbar, click " data audit " button, perhaps in " workflow " menu, select " data audit " option, can enter data audit interface.
The utility model excitation power source module with master control borad output+the 12V direct current changes into the 70V alternating current, for the EL backlight provides power supply.Lighting device uses the EL backlight, for the total system imaging provides light source; Sample is positioned over sample stage, and sample enters image collecting device by straight line guide rail transmission; The CCD digital camera device carries out individual or takes pictures continuously, finishes the collection of image; The imaging samples of taking transfers to master control borad; Master control borad is by the screening-mode of STC89C58 Single-chip Controlling CCD image pick-up device, and transfers to capture card through RS232 interface and AV terminal.Video frequency collection card cooperates analysis software to carry out analyzing and processing; Result after the analyzing and processing shows by PC, and can print operation; In addition, consider the clinical use actual conditions of each hospital, system also provides the interface with hospital HIS system and the WHONET5.3 of the World Health Organization (WHO).
Titer of antibodies analyser described in the utility model, its advantage is: 1, adopt the low power consumption of EL light source, luminous even, the life-span is permanent.2, modular design is easy to change and expansion.3, adopt height to separate 220 times of CCD digital camera devices of clear low-light (level), can carry out individual and take and shooting combination continuously, improved efficient.4, the ultraviolet disinfection function that has automatic control can be realized automatic sterilization, has solved the possibility of the cross-infection that exists in the shooting process.5, open data structure adapts to requirements of different users.5, the RS232 interface of standard can link to each other with hospital HIS system.
The utility model meets the requirement of NCCLS M35 Bacteria Identification and clinical common flora detects, and the bacterial strain total amount contains the common flora of clinical 70-90%, adapts to most hospital clinicals check demands.Need not special agent, saved the cost consumption of school greatly.Overcome antagonism or synergistic interference mutually between the susceptibility, from the direct interpretation of inhibition zone, report the result for 3 seconds fast; Expert system guarantees the direct applying clinical of result.WINDOWS operation interface easy to learn, Chinese reporting format directly perceived meets the personalized requirement of hospital fully.
Description of drawings
Fig. 1 is the Facad structure synoptic diagram of inhibition zone measurement mechanism described in the utility model;
Fig. 2 is the back side structural scheme of mechanism of inhibition zone measurement mechanism described in the utility model;
Fig. 3 is the inner structure synoptic diagram of inhibition zone measurement mechanism described in the utility model;
Fig. 4 is the theory diagram of the control/wiring of inhibition zone measurement mechanism described in the utility model;
Embodiment
The titer of antibodies analyser comprises inhibition zone measurement mechanism and peripheral hardware PC.
As Fig. 1, Fig. 2, Fig. 3, shown in Figure 4, the inhibition zone measurement mechanism comprises that one measures casing 1, referring to Fig. 1, in the front portion of measurement casing 1 control panel 2 is installed and has sample insertion mouth 3, control panel 2 is provided with power light 4, CCD LCD display lamp 5, shooting termination button 6, shooting startup button 7, sterilize pilot lamp 8, RESET reset button 9.
Referring to Fig. 2, the back side of measuring casing 1 be equipped with power switch 10, power interface 11 and etc. voltage ground hold 12.
Referring to Fig. 3, in measuring casing 1, chassis power supply 13, CCD monitor board 14, keypad 15, excitation power source plate 16, ultraviolet disinfecting 17, CCD digital camera device 18, backlight 19 and line slideway 20 are installed, sample stage 21 is inserted mouthfuls 3 from model and is inserted and measure in the casings 1 and be shelved on the line slideway 20, and CCD digital camera device 18 and backlight 19 lay respectively at the upper and lower of sample stage 21; External power supply inserts the input end of chassis power supply 13 by power interface 11 and power switch 10.
Provide 12V power supply referring to figure chassis power supply 13 to CCD monitor board 14, keypad 15, ultraviolet disinfecting 17, CCD monitor board 14 provides 12V power supply to CCD digital camera device 18, excitation power source plate 16; CCD monitor board 14 is connected with CCD digital camera device 18 by interior video line 22, by the screening-mode of the STC89C58 Single-chip Controlling CCD digital camera device 18 on it; Keypad 15 is connected with control panel 2, ultraviolet disinfecting 17, and is connected with CCD monitor board 14 by control data line 23; Excitation power source plate 16 comprises D/A module 24 and excitation power source module 25, and excitation power source module 25 changes into the 70V alternating current with the 12V direct current of CCD monitor board 14 outputs, for backlight 19 provides power supply; Also be provided with video frequency collection card 26 in the peripheral hardware PC, video frequency collection card 26 is connected with RS232 interface 30 with the AV terminal 29 of CCD monitor board 14 respectively with data line 28 by external video line 27.
The automatic Survey Software of inhibition zone is installed in the peripheral hardware PC.
Above-mentioned CCD digital camera device 18 adopts CD-64G2HP CCD module, and the shooting imaging samples transfers to builds control panel.1/3 inch colour, high parsing, low-light (level), 220 times of zoom CCD.
Its performance parameter is as follows:
1/4 inch of sensor
Total pixel 795 (level) * 596 (vertically) (CCIR standard);
811 (level) * 508 (vertically) (EIA standard)
Sao Miao Xi System 625 lines, 50/second (CCIR standard);
525 lines, 60/second (EIA standard)
Synchro system inter-sync/vertical synchronization locking
Minimal illumination 0.1Lux (F1.2,5600 ° of K)
(0.002Lux F1.2,5600 ° of K) (starlight pattern)
Horizontal definition 600 lines
Gain control mode automatic gain control (ON/OFF selects control by the screen menu)
Gain control range 0-18dB
Signal to noise ratio (S/N ratio) 48Db (minimum)/52dB (maximum)
(automatic gain is closed, r=1, and APC closes, Y-OUT)
Electronic shutter is automatic: 1/50 ~ 100,000 second continuously.
Manually: 8 grades
22 times of optical loupes heads of camera lens and visual angle F1.6 ~ 3.8, f=4.0 ~ 88.0mm
220 times of enlarging functions (22 times of optics X10 multiple words)
Motor variable (amplification, focusing, aperture)
500,000 life-span stepper motors of overlength
Focus on automatic/hand
Control interface Dry switch, RS-232C (driving optional)
Video output Fu Heshi ﹠amp; The output of separate type signal of video signal
1.0Vp-p?at?75ohm
64 groups of states of storing state (amplifying and focusing)
9600 bps of communication speeds
Gamma correction 0.45
Auto iris is controlled automatically/is fixed
Operating ambient temperature-20 ℃ is to+50 ℃
Below the working environment humidity 85%RH
12 volts ± 1 volt of power supply direct current
300 milliamperes of power consumptions.
Above-mentioned chassis power supply meets the medicine equipment safety standard.Input voltage: alternating current 220V-240V; 50-60Hz, output voltage :+12V, 2A; + 5V, 1A.
Above-mentioned backlight 19 provides light source for the total system imaging, and its parameter is as follows:
Operating voltage 30-250VAC
Frequency of operation 50-5000HZ
Static capacity 0.42nf/cm2min 400Hz
Electric current 0.14mA/cm2max 110V400Hz
Power consumption 2mw/cm2max 110V400Hz
Constant temperature high humidity life-span 200Hr/50cd/m250 ℃ 90%RH110V400Hz
Size 18cm * 18cm
Above-mentioned video frequency collection card is supported color (24)/super color (32) of black and white gray scale/height color (16)/very.Support image DDIB/DBUFFER/PREVIEW/OVERLAY, display mode/supports one-telephone multi-card, every card all can independently be provided with parameter/support real-time captions, figure to superpose.Pay and have the secondary development software package, support VB, VC, DELPHI, CB.
The performance parameter of above-mentioned titer of antibodies analyser is as follows:
This product of the scope of application is applicable to that the bacterial drug resistance that adopts scraps of paper agar diffusion experiments (K-B method) to carry out detects.Analysis time about 3 seconds/sample
Detect the inhibition zone on the principle CCD imaging system shooting double dish, output image information is through professional software analysis and automatic differentiation result.
Performance index
Measure antibacterial circle diameter Φ 0-48mm
Measurement range (visual field) 100mm
Measurement Resolution＜1%
Measuring accuracy≤1% of tiring
Repeatability CV≤0.2%
Safety analysis instrument safety requirements is by the requirement of I class Type B conventional equipment
Environmental baseline
Storing temperature :-20 to 60 ℃
Operating temperature: 10 to 30 ℃
Storage humidity: 15 to 90% (uncooled)
Operational humidity: 30 to 85% (uncooled)
Storage atmosphere is pressed: 700 to 1060hPa
Operation atmospheric pressure: 700 to 1060hPa
Condition of work
Room temperature: 10 ℃-30 ℃;
Indoor relative humidity is no more than 85%;
Power supply voltage: 220V ± 22V; 50Hz ± 1Hz;
Instrument should place stably on the worktable, must not have sunlight direct projection and intense electromagnetic field to disturb; Environment for use planted agent's non-corrosiveness gas;
Power supply should have good ground connection.
Power supply
Alternating current 220V-240V (± 10%); 50-60Hz (± 2%)
Power input :≤100VA ± 10%
Claims (2)
1, the titer of antibodies analyser, comprise inhibition zone measurement mechanism and peripheral hardware PC, it is characterized in that: described inhibition zone measurement mechanism comprises that one measures casing, in the front portion of measuring casing control panel is installed and has model insertion mouth, the back side of measuring casing is equipped with power switch, power interface and etc. voltage ground end, in measuring casing, chassis power supply is installed, the CCD monitor board, keypad, the excitation power source plate, ultraviolet disinfecting, the CCD digital camera device, backlight and line slideway, sample stage is inserted mouthful to insert from described model and is measured in the casing and be shelved on the line slideway, and CCD digital camera device and backlight lay respectively at the upper and lower of sample stage; External power supply is by the input end of power interface and power switch access chassis power supply, and chassis power supply provides 12V power supply to CCD monitor board, keypad, ultraviolet disinfecting, and the CCD monitor board provides 12V power supply to CCD digital camera device, excitation power source plate; The CCD monitor board is connected with the CCD digital camera device by the interior video line, by the screening-mode of the STC89C58 Single-chip Controlling CCD digital camera device on it; Keypad is connected with control panel, ultraviolet disinfecting, and is connected with the CCD monitor board by the control data line; The excitation power source plate comprises D/A module and excitation power source module, and the excitation power source module changes into the 70V alternating current with the 12V direct current of backlight CCD monitor board output, for backlight provides power supply; Also be provided with video frequency collection card in the peripheral hardware PC, video frequency collection card is connected with the RS232 interface with the AV terminal of CCD monitor board respectively with data line by the external video line.
2, titer of antibodies analyser according to claim 1 is characterized in that: described control panel is provided with power light, CCD LCD display lamp, shooting termination button, takes startup button, sterilization pilot lamp, RESET reset button.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520045092 CN2881620Y (en) | 2005-09-16 | 2005-09-16 | Automatic analyser for antibiotic titre |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520045092 CN2881620Y (en) | 2005-09-16 | 2005-09-16 | Automatic analyser for antibiotic titre |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2881620Y true CN2881620Y (en) | 2007-03-21 |
Family
ID=37881464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520045092 Expired - Fee Related CN2881620Y (en) | 2005-09-16 | 2005-09-16 | Automatic analyser for antibiotic titre |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2881620Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102297931A (en) * | 2011-05-20 | 2011-12-28 | 浙江大学 | Antibiotic titer determination method based on ink-jet printing principle |
| CN110243731A (en) * | 2019-07-03 | 2019-09-17 | 苏州新实医疗科技有限公司 | Antibacterial circle diameter dynamic measurement method, measuring device and readable storage medium storing program for executing |
-
2005
- 2005-09-16 CN CN 200520045092 patent/CN2881620Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102297931A (en) * | 2011-05-20 | 2011-12-28 | 浙江大学 | Antibiotic titer determination method based on ink-jet printing principle |
| CN102297931B (en) * | 2011-05-20 | 2013-11-20 | 浙江大学 | Antibiotic titer determination method based on ink-jet printing principle |
| CN110243731A (en) * | 2019-07-03 | 2019-09-17 | 苏州新实医疗科技有限公司 | Antibacterial circle diameter dynamic measurement method, measuring device and readable storage medium storing program for executing |
| CN110243731B (en) * | 2019-07-03 | 2021-09-24 | 苏州新实医疗科技有限公司 | Method and device for dynamically measuring diameter of bacteriostatic zone and readable storage medium |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |