CN2869867Y - Colloid gold test-paper for quick detecting propisochlor and metolachlor residue - Google Patents
Colloid gold test-paper for quick detecting propisochlor and metolachlor residue Download PDFInfo
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- CN2869867Y CN2869867Y CN 200520142623 CN200520142623U CN2869867Y CN 2869867 Y CN2869867 Y CN 2869867Y CN 200520142623 CN200520142623 CN 200520142623 CN 200520142623 U CN200520142623 U CN 200520142623U CN 2869867 Y CN2869867 Y CN 2869867Y
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- propisochlor
- isopropyl methoxalamine
- test paper
- monoclonal antibody
- colloid gold
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Abstract
A test paper of rapid detecting residuas of propisochlor and metolachlor, which comprises a base plate, a water absorption board, a pyroxylin membrane, a monoclonal antibody gold label mat and a specimen imbibition layer. The middle of the base plate is a pyroxylin membrane on which is provided with propisochlor synthetic immunogen test lead or metolachlorsynthetic immunogen test lead and a multi- clone antibody control line; one end tip of the base plate is provided with a water absorption board, and the other end tip is provided with a specimen imbibition layer; the two ends of pyroxylin membrane are separately overlapped for connection each other with the water absorption board and the monoclonal antibody gold label mat; the top of the monoclonal antibody gold label mat is pressed with a specimen imbibition layer; the test paper is made by means of a colloidal gold immunity competition method; the immunity colloidal gold method is used to detect if the propisochlor or metolachlor level of residue in garden stuff is overproof. The method has the features of speediness, sensitivity, simplicity of operation, low cost, no necessity of detecting by professional, real attainment of integrating the complicated principle with the simplicity of operation organically, as well as convenience of preservation and long period of validity.
Description
Technical field
The present invention relates to a kind of fruits and vegetables and raw grain based food propisochlor, isopropyl methoxalamine residue detection test paper and detection method thereof of being used for, belong to the colloid gold immune detection range.
Background technology
Because the vegetable growth phase is short and disease and pest is comparatively serious, thereby, in the growing vegetables process, usually need the continuous several times dispenser.Replace artificial weeding with the herbicide weeding in the modernization growing vegetables, raise labour efficiency.And many vegetables need repeatedly be gathered, gather and the time interval of dispenser short, cause when gathering the persticide residue in the vegetables often higher, be easy to surpass state specified standards.And persticide residue exceeds standard and can have a strong impact on level of human health in the vegetables, has become the food pollution source that can not be ignored.Government and each functional department extremely pay close attention to the residues of pesticides problem of food.But because the resistance to the action of a drug of the toxicity of agricultural chemicals and insect forms a vicious cycle, forbid thoroughly comprehensively that therefore the use of high highly toxic pesticide will have long process, even in using, low-toxin farm chemicals comprehensively, the pernicious poisoning of agricultural chemicals still takes place.Therefore new requirement and higher standard have all been proposed all many-sides such as the object of analyzing and testing, kind, quantity, scope, index.
Propisochlor, isopropyl methoxalamine are low toxicity herbicide, and light is difficult for decomposing, and to the skin minimal irritation, are listed in the important detected object of residues of pesticides.Propisochlor (2-chloro-N-(2-ethyl-6-methylbenzene)-N-[(1-methyl ethoxy) methyl] acetamide), the selectivity acetamide-group herbicides can be absorbed by plant plumule, enter Profilin enzymic synthesis in the plant.Early stage soil spraying processing behind the bud can be prevented and kill off corn, soybean, potatoes and other crops Tanaka's annual gramineous weed and some broad leaved weed before the bud.Isopropyl methoxalamine (2-ethyl-6-methyl-N-(1 '-methyl-2 '-methoxyethyl) chloro acetanilide), the selectivity acetamide-group herbicides, the effect characteristics are similar to propisochlor.Be applicable to dry crop weedings such as peanut, soybean, vegetables, corn, sugarcane, melon ground, mainly prevent and kill off lady's-grass, eleusine indica, green bristlegrass and purslane, emarginate amaranth herb or seed, cyperus iria, chufa etc.
At present, the analytical approach of detection residues of pesticides mainly is dependence, vapor-phase chromatography (GC) or gas chromatography and mass spectrum (MS) coupling method (GC/MS)." enzyme inhibition rate method+spectrophotometric method " has been listed in national proposed standard (GB/T 5009.199-2003).This method is quick, sensitive, easy and simple to handle and with low cost, can realize quick primary dcreening operation, but the restriction of examined scope and precision.And vapor-phase chromatography (GC) can realize the quantitative test of precision but speed is slower, and it is higher to detect cost.In a word, these method ubiquities the sample pretreatment process complexity, sensitivity be subjected to sample purification, step such as concentrate influence very big, consuming time, checkout equipment costliness, and require to have technical professional and long analytical cycle.
Therefore, utilize the competition law principle, on the basis of traditional immunodetection, introduced the collaurum Fast Detection Technique, develop propisochlor, isopropyl methoxalamine collaurum competition law detection test paper.This method has fast, sensitive, easy and simple to handle, cost is low, need not the professional detects, and really reaches the organic unity of complicated principle and ease of Use, have simultaneously preserve convenient, the characteristics that the term of validity is long.
Summary of the invention
The present invention is directed to some problems of above-mentioned existence, provide a kind of collaurum that is applicable to whether detection agricultural chemicals propisochlor, isopropyl methoxalamine exceed standard to measure test paper.
For solving the problems of the technologies described above, the present invention is achieved by the following technical solutions:
At Detecting Pesticide test paper base plate middle part is nitrocellulose filter, a test wire and a sheep anti mouse polyclonal antibody control line are arranged on the nitrocellulose filter, in base plate one end termination is water accepting layer, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with monoclonal antibody gold mark pad with water accepting layer respectively and are connected (the overlapping coupling part is in 1-2 millimeter scope), are pressed with the sample liquid-adsorption layer on monoclonal antibody gold mark pad.Combined method about base plate, nitrocellulose filter, thieving paper, collaurum mark pad is carried out by patent CN 2741052Y.
The control line of agricultural chemicals propisochlor or isopropyl methoxalamine residue detection test paper is formed by sheep anti mouse polyclonal antibody bag, test wire detect propisochlor be by propisochlor synthetic immunogen bag by, to detect isopropyl methoxalamine be by isopropyl methoxalamine synthetic immunogen bag quilt.The sample liquid-adsorption layer end that detects the residual colloid gold test paper of propisochlor or isopropyl methoxalamine is put into fruit juice, vegetables are soaked in the juice, because the capillarity sample will move along test strips water accepting layer end, when moving to monoclonal antibody gold mark pad, propisochlor in the sample and propisochlor monoclonal antibody gold mark probe, alachlor and isopropyl methoxalamine monoclonal antibody gold mark probe generation specific bond in different, be fixed with propisochlor synthetic immunogen test wire when moving to, during isopropyl methoxalamine synthetic immunogen test wire, because propisochlor in antibody in the propisochlor monoclonal antibody gold mark pad and the sample, isopropyl methoxalamine preferentially is combined into compound and loses it and combine with propisochlor or isopropyl methoxalamine synthetic immunogen in antibody in the isopropyl methoxalamine monoclonal antibody gold mark pad and the sample, therefore its collaurum can not be stranded on the test wire, the test wire place does not have red line to show, promptly has only a red control line positive; If do not have propisochlor, isopropyl methoxalamine in the opposite sample, antibody in the monoclonal antibody gold mark pad moves on the test wire, the propisochlor monoclonal antibody will with propisochlor synthetic immunogen, isopropyl methoxalamine monoclonal antibody will with isopropyl methoxalamine synthetic immunogen generation specific bond, collaurum is stranded on the test wire, promptly two red line are negative, immune competition law principle that Here it is.Propisochlor or isopropyl methoxalamine content just are inversely proportional in the shade of test wire and the sample.Carry out the transition to feminine gender from the positive, since the institute propisochlor of surveying or isopropyl methoxalamine content difference, the color of test wire also the difference, form a red color gradient difference, detect the OD value with this principle, thereby infer that test wire reacts the amount of actual propisochlor or isopropyl methoxalamine.
It detects more than the agricultural chemicals setting detected level to make test paper with competition law, and it is positive that a red line appears in the view window place; It is negative to set the two red lines of the following appearance of detected level; Be that fuzzy hacures are boundary value at the test wire place when setting detected level, thereby draw judgement.
When moving to sheep anti mouse polyclonal antibody control line, no matter have or not propisochlor, isopropyl methoxalamine in the sample, the gold mark probe of mark all can combine delay with the sheep anti mouse polyclonal antibody that has configured, and it is red that control line is shown.Therefore control line does not have colour band and produces that then the representative operation is wrong, during detection the sample liquid level surpass the MAX line or test paper expired.
Because adopt technique scheme, a kind of fast detecting propisochlor provided by the present invention, the residual colloid gold test paper of isopropyl methoxalamine have such beneficial effect, i.e. high specificity, highly sensitive, easily store, need not the technical skill personnel operation, and readability as a result.
Description of drawings
Fig. 1 is the main TV structure figure of the residual colloid gold test paper of a kind of fast detecting propisochlor of the present invention, isopropyl methoxalamine.
Fig. 2 is the side-looking structural drawing of invention Fig. 1.
Fig. 3 shows positive findings figure for detecting.
Fig. 4 shows negative findings figure for detecting.
Among the figure 1, water sucting plate, 2, nitrocellulose filter, 3, sheep anti mouse polyclonal antibody control line, 4, test wire, 5, monoclonal antibody gold mark pad, 6, the sample liquid-adsorption layer, 7, base plate, 8, the MAX line.
Specific embodiment
1. according to the propisochlor molecular structure, infer the antigen determining area in its molecule, select for use the propisochlor pesticide original medicine synthetic a kind of close with the propisochlor molecular structure and have carboxyl or amino etc. and be easy to the be connected molecular structure (haptens) of group of protein macromolecule through chemical reaction.The haptens micromolecule is connected with protein macromolecule makes the propisochlor immunizing antigen.The same propisochlor of isopropyl methoxalamine antigen preparation method.
2. MONOCLONAL ANTIBODIES SPECIFIC FOR
(1) with the propisochlor synthetic immunogen, the isopropyl methoxalamine synthetic immunogen immunity BALB/c mouse that prepare.Set up the knurl strain with the SP2/0 Fusion of Cells and screen, get oncocyte and be injected in the BALB/c mouse abdominal cavity, make it produce ascites.
(2) extract the screening of mouse ascites purifying, the monoclonal antibody that acquisition can combine with propisochlor is used for colloid gold label.The same propisochlor of isopropyl methoxalamine method for preparing monoclonal antibody.
The preparation of collaurum and with the combining of monoclonal antibody
(1) get distilled water and add an amount of gold chloride magnetic agitation and be warmed to 85～95 ℃, add an amount of citrate three sodium and continue heated and stirred to seething with excitement 3～10 minutes, it is standby to keep in Dark Place after the cooling.
(2) the collaurum liquid of propisochlor labeling of monoclonal antibody, on the adsorbing fiber material, dry back is standby.
The same propisochlor of isopropyl methoxalamine monoclonal antibody association colloid gold method.
4. film-making machine system film: utilize computer control transmission speed, guarantee that the antibody amount of bag quilt on the per unit film equates.
5. test strips combination: see Figure of description Fig. 2, method is with patent CN 2741052Y.
6. using method: the sample liquid-adsorption layer end of test paper is put into vegetables soak juice (liquid level must not surpass MAX line chart 8), take out test paper after 1 minute and keep flat, because kapillary and syphonic effect sample will move observations in the time of 5 minutes along test strips water accepting layer end.
7. the result judges: if the limit of identification of this test paper is set at 0.1mg/kg, then detect more than the propisochlor concentration 0.1mg/kg, it is positive that a red line appears in the view window place; 0.1mg/kg the two red lines of following appearance are negative; 0.1mg/kg the time be that fuzzy hacures are boundary value at the test wire place, this boundary value is the vegetable pesticide residue examination criteria 0.1mg/kg of country's promulgation.Thereby draw judgement.Being lower than the following boundary value of 0.1mg/kg takes the colour code control methods to judge dosage.
Claims (6)
1. a fast detecting propisochlor, the residual colloid gold test paper of isopropyl methoxalamine is characterized in that being made up of base plate (7), water sucting plate (1), nitrocellulose filter (2), propisochlor or isopropyl methoxalamine monoclonal antibody gold mark pad (5), sample liquid-adsorption layer (6), MAX line (8); The base plate middle part is a nitrocellulose filter, a test wire (4) and a polyclonal antibody control line (3) are arranged on the nitrocellulose filter, base plate one end termination is a water sucting plate, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the sample liquid-adsorption layer on monoclonal antibody gold mark pad.
2. the residual colloid gold test paper of colloid gold test paper, isopropyl methoxalamine that a kind of fast detecting propisochlor according to claim 1 is residual is characterized in that control line is to be made into by sheep anti mouse polyclonal antibody bag.
3. a kind of fast detecting propisochlor according to claim 1, the residual colloid gold test paper of isopropyl methoxalamine is characterized in that test wire is to be made into by propisochlor or isopropyl methoxalamine synthetic immunogen bag.
4. according to claim 1 or 3 described a kind of fast detecting propisochlors, the residual colloid gold test paper of isopropyl methoxalamine, it is characterized in that this test paper can detect propisochlor, the former medicine of isopropyl methoxalamine or potpourri according to the composition of regulating gold mark pad and test wire encrusting substance.
5. a kind of fast detecting propisochlor according to claim 1, the residual colloid gold test paper of isopropyl methoxalamine is characterized in that gold mark pad is as colloid gold label by propisochlor monoclonal antibody, isopropyl methoxalamine monoclonal antibody.
6. according to the described a kind of fast detecting propisochlor of claim 1, the residual colloid gold test paper of isopropyl methoxalamine, it is propisochlor 0.02～0.5mg/kg, isopropyl methoxalamine 0.5～1mg/kg that the detected value that it is characterized in that test wire is set at limit of identification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520142623 CN2869867Y (en) | 2005-12-06 | 2005-12-06 | Colloid gold test-paper for quick detecting propisochlor and metolachlor residue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520142623 CN2869867Y (en) | 2005-12-06 | 2005-12-06 | Colloid gold test-paper for quick detecting propisochlor and metolachlor residue |
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| CN2869867Y true CN2869867Y (en) | 2007-02-14 |
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| CN 200520142623 Expired - Fee Related CN2869867Y (en) | 2005-12-06 | 2005-12-06 | Colloid gold test-paper for quick detecting propisochlor and metolachlor residue |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105486823A (en) * | 2014-10-13 | 2016-04-13 | 江苏维赛科技生物发展有限公司 | Enzyme linked immunosorbent assay kit for detecting pretilachlor and detection method thereof |
| CN105572360A (en) * | 2014-10-12 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immune colloidal gold detection card of pretilachlor, and making method thereof |
| CN112462070A (en) * | 2020-11-13 | 2021-03-09 | 北京望尔生物技术有限公司 | Test strip for detecting alachlor and application thereof |
-
2005
- 2005-12-06 CN CN 200520142623 patent/CN2869867Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105572360A (en) * | 2014-10-12 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immune colloidal gold detection card of pretilachlor, and making method thereof |
| CN105486823A (en) * | 2014-10-13 | 2016-04-13 | 江苏维赛科技生物发展有限公司 | Enzyme linked immunosorbent assay kit for detecting pretilachlor and detection method thereof |
| CN112462070A (en) * | 2020-11-13 | 2021-03-09 | 北京望尔生物技术有限公司 | Test strip for detecting alachlor and application thereof |
| CN112462070B (en) * | 2020-11-13 | 2023-07-11 | 北京望尔生物技术有限公司 | Test strip for detecting alachlor and application thereof |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070214 Termination date: 20100106 |