CN2844922Y - Gel gold test paper for quick inspecting remained 3,5,6-trichlorodipyridine-one - Google Patents
Gel gold test paper for quick inspecting remained 3,5,6-trichlorodipyridine-one Download PDFInfo
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- CN2844922Y CN2844922Y CN 200520142603 CN200520142603U CN2844922Y CN 2844922 Y CN2844922 Y CN 2844922Y CN 200520142603 CN200520142603 CN 200520142603 CN 200520142603 U CN200520142603 U CN 200520142603U CN 2844922 Y CN2844922 Y CN 2844922Y
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- trichlorine
- test paper
- alcohol
- monoclonal antibody
- pyridines
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Abstract
The utility model relates to a piece of colloidal gold test paper for rapidly detecting 3, 5, 6-trichloro-2-pyridinol residues, which is composed of a bottom plate, a water absorbing plate, a nitrocellulose film, a monoclonal antibody gold label pad and a sample liquid absorbing layer, wherein the middle part of the bottom plate is provided with the nitrocellulose film. A chlorpyrifos degradation object 3, 5, 6-trichloro-2-pyridinol synthetic immunogen test line and a polyclonal antibody control line are arranged on the nitrocellulose film. The end head at one end of the bottom plate is provided with the water absorbing plate, and the end head at the other end is provided with the sample liquid absorbing layer. Both ends of the nitrocellulose film are respectively and mutually overlapped and connected with the water absorbing plate and the monoclonal antibody gold label pad. The sample liquid absorbing layer is pressed on the monoclonal antibody gold label pad. The test paper is made through the immunization competing method of colloidal gold. The immunization colloidal gold method is used for detecting whether the residual quantity of 3, 5, 6-trichloro-2-pyridinol in fruit vegetable is overproof or not, thereby, whether chlorpyrifos pesticide residues are overproof can be judged. The method has the advantages of rapidity, sensitivity, convenient operation and low cost. The utility model does not need to be detected by professionals, and the organic unification of complicated theory and simple operation can be really achieved. Simultaneously, the utility model has the characteristics of convenient preservation and long effective period.
Description
Technical field
The present invention relates to a kind of fruits and vegetables and raw grain based food Detecting Pesticide test paper and detection method thereof of being used for, belong to the colloid gold immune detection range.
Background technology
Because the vegetable growth phase is short and disease and pest is comparatively serious, thereby, in the growing vegetables process, usually need the continuous several times dispenser.And many vegetables need repeatedly be gathered, gather and the time interval of dispenser short, cause when gathering the persticide residue in the vegetables often higher, be easy to surpass state specified standards.And persticide residue exceeds standard and can have a strong impact on level of human health in the vegetables, has become the food pollution source that can not be ignored.Government and each functional department extremely pay close attention to the residues of pesticides problem of food.But because the resistance to the action of a drug of the toxicity of agricultural chemicals and insect forms a vicious cycle, forbid thoroughly comprehensively that therefore the use of high highly toxic pesticide will have long process, even in using, low-toxin farm chemicals comprehensively, the pernicious poisoning of agricultural chemicals still takes place.Therefore new requirement and higher standard have all been proposed all many-sides such as the object of analyzing and testing, kind, quantity, scope, index.
3,5,6-trichlorine two pyridine alcohol are main degradation products of pesticide chlopyrifos, and it is that physical property or chemical property all are different from chlopyrifos, and the researcher thinks 3,5 in the last thirty years, and 6-trichlorine two pyridine alcohol are the marks that detect the pesticide chlopyrifos.Chlopyrifos (O-O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate) is a kind of current use moderate toxicity organophosphorus pesticide widely.Because it may injure children's nervous system; stop the growth of brain, Environmental Protection Agency (EPA) declaration on June 8th, 2000 stops the use of chlopyrifos in dwelling house (comprising dwelling house meadow and garden), school, nursery, park, hospital, private rest home and dwelling house meadow, shopping center and garden at the year ends 2000.Ministry of Health of the People's Republic of China and Standardization Administration of China issued on January 25th, 2005, implemented agricultural chemicals maximum residue limit (GB 2763-2005) in State Standard of the People's Republic of China's food on October 1st, 2005, standard defines the maximum of agricultural chemicals chlopyrifos in crops and allows residual quantity.
At present, the analytical approach of detection residues of pesticides mainly is dependence, vapor-phase chromatography (GC) or gas chromatography and mass spectrum (MS) coupling method (GC/MS)." enzyme inhibition rate method+spectrophotometric method " has been listed in national proposed standard (GB/T 5009.199-2003).This method is quick, sensitive, easy and simple to handle and with low cost, can realize quick primary dcreening operation, but the restriction of examined scope and precision.And vapor-phase chromatography (GC) can realize the quantitative test of precision but speed is slower, and it is higher to detect cost.In a word, these method ubiquities the sample pretreatment process complexity, sensitivity be subjected to sample purification, step such as concentrate influence very big, consuming time, checkout equipment costliness, and require to have technical professional and long analytical cycle.
Therefore, utilize the competition law principle, introduced the collaurum Fast Detection Technique on the basis of traditional immunodetection, develop chlorpyrifos degrading thing 3,5,6-trichlorine two pyridines alcohol collaurum competition law detects test paper.This method has fast, sensitive, easy and simple to handle, cost is low, need not the professional detects, and really reaches the organic unity of complicated principle and ease of Use, have simultaneously preserve convenient, the characteristics that the term of validity is long.
Summary of the invention
The present invention is directed to some problems of above-mentioned existence, provide a kind of being applicable to detect chlorpyrifos degrading thing 3,5, the collaurum whether 6-trichlorine two pyridine alcohol exceed standard is measured test paper.
For solving the problems of the technologies described above, the present invention is achieved by the following technical solutions:
At chlorpyrifos degrading thing 3,5,6-trichlorine two pyridines alcohol residue detection test paper base plate middle part is a nitrocellulose filter, a test wire and a sheep anti mouse polyclonal antibody control line are arranged on the nitrocellulose filter, in base plate one end termination is water accepting layer, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends respectively with water accepting layer and chlorpyrifos degrading thing 3,5,6-trichlorine two pyridines alcohol monoclonal antibody gold mark pad overlaps mutually and connects (the overlapping coupling part is in 1--2 millimeter scope), at chlorpyrifos degrading thing 3,5, be pressed with the sample liquid-adsorption layer on the 6-trichlorine two pyridines alcohol monoclonal antibody gold mark pad.Combined method about base plate, nitrocellulose filter, thieving paper, collaurum mark pad is carried out by patent CN 2741052Y.
The limit of identification of test wire can reach chlorpyrifos degrading thing 3,5, and 6-trichlorine two pyridines alcohol content is 0.5mg/kg.The garden stuff pesticide residue examination criteria chlopyrifos content that the detected value of test wire is set at country's promulgation is that 0.5mg/kg or granule fruit Detecting Pesticide standard chlopyrifos content are that 1mg/kg or raw grain Detecting Pesticide standard chlopyrifos content are 0.1mg/kg.
It detects chlorpyrifos degrading thing 3,5 to make test paper with competition law, and 6-trichlorine two pyridines alcohol is set more than the detected level, and it is positive that a red line appears in the view window place; It is negative to set the two red lines of the following appearance of detected level; Be that fuzzy hacures are boundary value at the test wire place when setting detected level, thereby draw judgement.
When moving to sheep anti mouse polyclonal antibody control line, no matter have or not chlorpyrifos degrading thing 3,5 in the sample, 6-trichlorine two pyridine alcohol, the gold mark probe of mark all can combine delay with the sheep anti mouse polyclonal antibody that has configured, and it is red that control line is shown.Therefore control line does not have colour band and produces that then the representative operation is wrong, during detection the sample liquid level surpass the MAX line or test paper expired.
Owing to adopt technique scheme, a kind of fast detecting chlorpyrifos degrading thing 3,5 provided by the present invention, the residual colloid gold test paper of 6-trichlorine two pyridines alcohol has such beneficial effect, and promptly high specificity is highly sensitive, easily store, need not the technical skill personnel operation, and readability as a result.
Description of drawings
Fig. 1 is a kind of fast detecting chlorpyrifos degrading of the present invention thing 3,5, the main TV structure figure of the colloid gold test paper that 6-trichlorine two pyridines alcohol is residual.
Fig. 2 is the side-looking structural drawing of invention figure one.
Fig. 3 shows positive findings figure for detecting.
Fig. 4 shows negative findings figure for detecting.
Among the figure 1, water sucting plate, 2, nitrocellulose filter, 3, sheep anti mouse polyclonal antibody control line, 4, test wire, 5, monoclonal antibody gold mark pad, 6, the sample liquid-adsorption layer, 7, base plate, 8, the MAX line.
Specific embodiment
1. according to chlorpyrifos degrading thing 3,5, the molecular structure of 6-trichlorine two pyridine alcohol, infer the antigen determining area in its molecule, 3,5,6-trichlorine two pyridines alcohol pesticide intermediate is synthetic a kind of and chlorpyrifos degrading thing 3 through chemical reaction, 5,6-trichlorine two pyridines alcohol molecular structure close and have carboxyl or amino etc. and be easy to the be connected molecular structure (haptens) of group of protein macromolecule.The haptens micromolecule is connected with protein macromolecule makes chlorpyrifos degrading thing 3,5,6-trichlorine two pyridines alcohol immunizing antigen.
2. MONOCLONAL ANTIBODIES SPECIFIC FOR
(1) with synthetic immunogen immune BALB/c mouse.Set up the knurl strain with the SP2/0 Fusion of Cells and screen, get oncocyte and be injected in the BALB/c mouse abdominal cavity, make it produce ascites.
(2) extract the screening of mouse ascites purifying, acquisition can be with 3,5, and the monoclonal antibody of 6-trichlorine two pyridines alcohol molecule combination is used for colloid gold label.
The preparation of collaurum and with the combining of monoclonal antibody
(1) get distilled water and add an amount of gold chloride magnetic agitation and be warmed to 90～95 ℃, add an amount of citrate three sodium and continue heated and stirred to seething with excitement 5 minutes, it is standby to keep in Dark Place after the cooling.
(2) 3,5, the collaurum liquid of 6-trichlorine two pyridines alcohol labeling of monoclonal antibody, on the adsorbing fiber material, dry back is standby.
4. film-making machine system film: utilize computer control transmission speed, guarantee that the antibody amount of bag quilt on the per unit film equates.
5. test strips combination: see Figure of description Fig. 2, with patent CN 2741052Y.
6. using method: the sample liquid-adsorption layer end of test paper put into fruit juice, vegetables are soaked juice (liquid level must not surpass MAX line chart 8),
Kapillary and syphonic effect sample take out test paper after 1 minute and keep flat, owing to will move observations in the time of 5 minutes along test strips water accepting layer end.
7. the result judges: detect 3,5, more than the 6-trichlorine two pyridine determining alcohol 0.5mg/kg, it is positive that a red line appears in the view window place; 0.5mg/kg the two red lines of following appearance are negative; 0.5mg/kg the time be that fuzzy hacures are boundary value at the test wire place, this boundary value is the vegetable pesticide residue chlopyrifos examination criteria 0.5mg/kg of country's promulgation.Judge whether chlorpyrifos pesticide residue exceeds standard thereby draw.
Claims (6)
1. fast detecting 3,5, the colloid gold test paper that 6-trichlorine two pyridines alcohol is residual, it is characterized in that it being by base plate (7), water sucting plate (1), nitrocellulose filter (2), chlorpyrifos degrading 3,5,6-trichlorine two pyridines alcohol monoclonal antibody gold mark pad (5), sample liquid-adsorption layer (6), MAX line (8) are formed; The base plate middle part is a nitrocellulose filter, a test wire (4) and a polyclonal antibody control line (3) are arranged on the nitrocellulose filter, base plate one end termination is a water sucting plate, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the sample liquid-adsorption layer on monoclonal antibody gold mark pad.
2. a kind of fast detecting 3,5 according to claim 1, the colloid gold test paper that 6-trichlorine two pyridines alcohol is residual is characterized in that control line is to be made into by sheep anti mouse polyclonal antibody bag.
3. a kind of fast detecting 3,5 according to claim 1, the colloid gold test paper that 6-trichlorine two pyridines alcohol is residual is characterized in that test wire is by chlorpyrifos degrading thing 3,5,6-trichlorine two pyridines alcohol synthetic immunogen bag is made into.
4. according to claim 1 or 3 described a kind of fast detecting 3,5, the colloid gold test paper that 6-trichlorine two pyridines alcohol is residual, what it is characterized in that being detected is chlorpyrifos degrading thing 3,5,6-trichlorine two pyridine alcohol.
5. a kind of fast detecting 3,5 according to claim 1, the colloid gold test paper that 6-trichlorine two pyridines alcohol is residual is characterized in that gold mark pad is as colloid gold label by monoclonal antibody.
6. according to the described a kind of fast detecting 3,5 of claim 1, the colloid gold test paper that 6-trichlorine two pyridines alcohol is residual is characterized in that test wire limit of identification scope is set at 0.01～5mg/kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520142603 CN2844922Y (en) | 2005-12-06 | 2005-12-06 | Gel gold test paper for quick inspecting remained 3,5,6-trichlorodipyridine-one |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520142603 CN2844922Y (en) | 2005-12-06 | 2005-12-06 | Gel gold test paper for quick inspecting remained 3,5,6-trichlorodipyridine-one |
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| Publication Number | Publication Date |
|---|---|
| CN2844922Y true CN2844922Y (en) | 2006-12-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520142603 Expired - Fee Related CN2844922Y (en) | 2005-12-06 | 2005-12-06 | Gel gold test paper for quick inspecting remained 3,5,6-trichlorodipyridine-one |
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| CN (1) | CN2844922Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101469313B (en) * | 2007-09-18 | 2011-02-16 | 华南农业大学 | Chlopyrifos pesticide degradation bacteria, inocula and preparation thereof |
-
2005
- 2005-12-06 CN CN 200520142603 patent/CN2844922Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101469313B (en) * | 2007-09-18 | 2011-02-16 | 华南农业大学 | Chlopyrifos pesticide degradation bacteria, inocula and preparation thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20061206 Termination date: 20100106 |