CN2811997Y - Apparatus for preparing crosslinked polyacrylamide coated column for capillary electrophoresis - Google Patents
Apparatus for preparing crosslinked polyacrylamide coated column for capillary electrophoresis Download PDFInfo
- Publication number
- CN2811997Y CN2811997Y CN 200520091720 CN200520091720U CN2811997Y CN 2811997 Y CN2811997 Y CN 2811997Y CN 200520091720 CN200520091720 CN 200520091720 CN 200520091720 U CN200520091720 U CN 200520091720U CN 2811997 Y CN2811997 Y CN 2811997Y
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- reaction vessel
- container cover
- capillary electrophoresis
- helium
- hole
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Abstract
The utility model relates to the preparation facilities of Capillary Electrophoresis, and particularly a kind of preparation facilities of cross-linking type polyacrylamide type Capillary Electrophoresis coating column comprises reaction vessel and container cover, and the container cover sealing is inserted in the reaction vessel, and container cover is provided with a plurality of holes.The number in hole is four on the described container cover, is respectively capillary bore, relieving device, reagent wells and helium hole; Described reaction vessel is processed into by Teflon or glass or quartz or macromolecule transparent material; Be provided with variable valve between described helium hole and the helium gas source.Reaction vessel of the present utility model is that transparent material is made, and can do dynamic observing course of reaction; And can realize removing the oxygen that dissolves in the reactive polymeric liquid, and can prevent the dissolving once more of oxygen in polymerization process, and then reduce the reaction time, improve the repeatability of system post.
Description
Technical field
The utility model relates to the preparation facilities of Capillary Electrophoresis, particularly a kind of preparation facilities of cross-linking type polyacrylamide type Capillary Electrophoresis coating column.
Background technology
(capillary electrophoresis CE) has the separation efficiency height with respect to other isolation technics to high performance capillary electrophoresis, and analysis time is short, and sample consumes advantages such as low, has obtained in recent years developing rapidly.CE makes this system have great application prospect at present flourish protein science research to the high separating efficiency of big minute subsample of protein, polypeptide and other biological.CE analyzes basic protein subject matter and is: the silicon hydroxyl that untreated quartz capillary wall is residual, and the alkaline protein formation irreversible adsorption with positively charged makes the bands of a spectrum broadening, and holder tail in peak separates reappearance and significantly reduces.The resolution policy of this problem mainly contains dynamic deactivation method, wall coating and extreme PH method.Wherein, use the most general with the wall coating.Coating technology can adopt physisorption or chemical bonding dual mode, and wherein physical adsorption techniques (as methylcellulose or polyethyleneimine etc.) system post is simple, but stability and reappearance are relatively poor.And chemical bonding coating (as linear polyacrylamide, cross-linked polyacrylamide etc.) forms one deck permanent coating at capillary surface, can reduce electroosmotic flow effectively, reduces the absorption of albumen, has stability and reappearance preferably.In traditional cross-linked polyacrylamide coating column preparation system, owing to can't effectively driving away oxygen molecule or can't guarantee that in course of reaction the dissolving once more of oxygen molecule makes the reaction time tediously long, system post poor repeatability.
The utility model content
The purpose of this utility model is to provide a kind of both can remove the oxygen that dissolves in the reactive polymeric liquid, can prevent the preparation facilities of the cross-linking type polyacrylamide type Capillary Electrophoresis coating column of dissolving once more of oxygen in polymerization process again.
The purpose of this utility model is achieved through the following technical solutions:
The utility model comprises reaction vessel and container cover, and the container cover sealing is inserted in the reaction vessel, and container cover is provided with a plurality of holes.
Wherein: the number in hole is four on the described container cover, is respectively capillary bore, relieving device, reagent wells and helium hole; Described reaction vessel is processed into by Teflon or glass or quartz or macromolecule transparent material; Be provided with variable valve between described helium hole and the helium gas source.
Advantage of the present utility model and good effect are:
Reaction vessel of the present utility model is that transparent material is made, and can do dynamic observing course of reaction; And can realize removing the oxygen that dissolves in the reactive polymeric liquid, and can prevent the dissolving once more of oxygen in polymerization process, and then reduce the reaction time, improve the repeatability of system post.
Description of drawings
Fig. 1 is an one-piece construction synoptic diagram of the present utility model;
Fig. 2 is used for the capillary electrophoresis analysis basic protein for the coating column of the utility model preparation;
Fig. 3 is used for the capillary electrophoresis analysis Bitter Orang P.E for the coating column of the utility model preparation.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
As shown in Figure 1, the utility model comprises reaction vessel 1 and container cover 2, container cover 2 sealings are inserted in the reaction vessel 1, container cover 2 is provided with four holes, be respectively the helium hole 6 of the relieving device 4 of capillary bore 3, discharging oxygen and the helium of laying coatings capillary pipe, the reagent wells 5 of adding reagent and placement helium tube, each hole does not have fixing status requirement.Reaction vessel 1 is processed into by Teflon or glass or quartz or macromolecule transparent material.Be provided with variable valve 8 between helium hole 6 and the helium gas source, be used for regulating the pressure that injects reaction vessel 1 helium.
Preparation process comprises column wall pre-service and two steps of polyreaction.The column wall pre-treatment step comprises: kapillary 7 orders are cleaned with NaOH aqueous solution, pure water, acetone, put into gas chromatography stove case after the processing again, after 140 ℃ of nitrogen are blown down drying, to contain percent by volume and be 66% propyl methyl acid esters acetonitrile solution and be injected in this kapillary 7, in water-bath, react 2～3 hours.Used acetone rinsing then 1 hour, standby after 140 ℃ of nitrogen are blown down drying.
Polymerization procedure comprises: open container cover 2, acrylamide and methene base bisacrylamide mixed solution are poured in the reaction vessel 1, then with container cover 2 and reaction vessel 1 good seal.Inject helium by helium hole 6 in reaction vessel 1, acrylamide and the deoxygenation of methene base bisacrylamide mixed solution are handled half an hour, the gas in the reaction vessel 1 is discharged outside the reaction vessel 1 through relieving device 4.To handle well then, standby kapillary 7 inserts in the reaction vessels 1 through capillary bore 3, adds ammonium persulfate and N,N,N solution by reagent wells 5 in reaction vessel 1.Regulate variable valve 8 then, strengthen the pressure of helium, rapidly the solution in the reaction vessel 1 is pressed in the kapillary of having handled well 7, after 4～10 minutes with kapillary 7 by taking out in the reaction vessel 1, use purified rinse water, nitrogen dries up dry then.
Analysis condition when the coating column of preparing as shown in Figure 2, is used for the capillary electrophoresis analysis basic protein is: UV-detector on the Jasco CE-1575 post; Kapillary 40/34cm (length overall/effectively); KH
2PO
4Damping fluid 0.02mol/L, PH=4.0; Separation voltage 10KV; Separate electric current 25 μ A; Siphon sample introduction 10cm * 15s.9 is cromoci among the figure; 10 is lysozyme (lysozyme); 11 is ribonuclease A.
As shown in Figure 3, the electrophoretic separation condition that the coating column of preparation is used for the capillary electrophoresis analysis Bitter Orang P.E is: separate electric current 26 μ A, the analysis condition of other condition during with the analysis basic protein is identical.Among the figure, 12 is the unknown material peak; 13 is synephrine.
Claims (4)
1. the preparation facilities of a cross-linking type polyacrylamide type Capillary Electrophoresis coating column is characterized in that: comprise reaction vessel (1) and container cover (2), container cover (2) sealing is inserted in the reaction vessel (1), and container cover (2) is provided with a plurality of holes.
2. press the preparation facilities of the described a kind of cross-linking type polyacrylamide type Capillary Electrophoresis coating column of claim 1, it is characterized in that: the number that described container cover (2) goes up the hole is four, is respectively capillary bore (3), relieving device (4), reagent wells (5) and helium hole (6).
3. by the preparation facilities of the described a kind of cross-linking type polyacrylamide type Capillary Electrophoresis coating column of claim 1, it is characterized in that: described reaction vessel (1) is processed into by Teflon or glass or quartz or macromolecule transparent material.
4. by the preparation facilities of the described a kind of cross-linking type polyacrylamide type Capillary Electrophoresis coating column of claim 1, it is characterized in that: be provided with variable valve (8) between described helium hole (6) and the helium gas source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520091720 CN2811997Y (en) | 2005-07-08 | 2005-07-08 | Apparatus for preparing crosslinked polyacrylamide coated column for capillary electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520091720 CN2811997Y (en) | 2005-07-08 | 2005-07-08 | Apparatus for preparing crosslinked polyacrylamide coated column for capillary electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2811997Y true CN2811997Y (en) | 2006-08-30 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520091720 Expired - Fee Related CN2811997Y (en) | 2005-07-08 | 2005-07-08 | Apparatus for preparing crosslinked polyacrylamide coated column for capillary electrophoresis |
Country Status (1)
| Country | Link |
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| CN (1) | CN2811997Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754873A (en) * | 2016-12-05 | 2017-05-31 | 四川大学 | DNA separating kits |
| CN109716122A (en) * | 2016-08-15 | 2019-05-03 | 塔斯马尼亚大学 | Inorganic ions detection system and method |
-
2005
- 2005-07-08 CN CN 200520091720 patent/CN2811997Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109716122A (en) * | 2016-08-15 | 2019-05-03 | 塔斯马尼亚大学 | Inorganic ions detection system and method |
| CN109716122B (en) * | 2016-08-15 | 2022-06-24 | 塔斯马尼亚大学 | Inorganic ion detection system and method |
| CN106754873A (en) * | 2016-12-05 | 2017-05-31 | 四川大学 | DNA separating kits |
| CN106754873B (en) * | 2016-12-05 | 2019-11-26 | 四川大学 | DNA separating kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060830 |