CN2793720Y

CN2793720Y - Biological chip scanner

Info

Publication number: CN2793720Y
Application number: CN 200520011600
Authority: CN
Inventors: 郑荣松; 蔡孟达
Original assignee: QUANYOU COMPUTER STOCK-SHARING Co Ltd
Current assignee: QUANYOU COMPUTER STOCK-SHARING Co Ltd
Priority date: 2005-04-11
Filing date: 2005-04-11
Publication date: 2006-07-05
Anticipated expiration: 2015-04-11

Abstract

The utility model relates to a biological chip scanner which comprises a light source capable of emitting light, a rotatable raster-shaped full photo, a sampling bench, and a line style charge coupled device (CCD), wherein one side of the light source is provided with the rotatable raster-shaped full photo, and diffraction can be generated by an incident light to form a diffraction light; one end of the raster-shaped full photo is provided with the sampling bench, and a sampling specimen is arranged above the sampling bench; the line style charge coupled device (CCD) can receive light rays which are generated through that the diffraction light irradiates to the sampling sample and is reflected. The rotatable raster-shaped full photo leads the incident light rays to generate uniform diffraction, and thus, the correct signal noise ratio can be obtained to ensure the correctness of signals of the light rays. In addition, the light source can be replaced by a linear light source and the efficacies of enhancing scanning speed and reducing time expend are obtained.

Description

Biological Crystal Plate Scanner

Technical field

The utility model is relevant with a kind of biochip scanning apparatus, particularly about a kind of true signal noise ratio of calibration that obtains, to guarantee the biochip scanning apparatus of light signal correctness.

Background technology

Biotechnology is at a tremendous pace, and especially the detecting of DNA all is very important information source to human inheritance, disease and identification, and the tracking of the prevention of infantile tumour and medicine effect is all accompanied drilling pivotal player.Therefore, the accuracy of DNA Detecting device and accuracy just form necessary conditions.Basically, the DNA Detecting device is the biochip scanner, its principle is when in the dna sample coating test piece, be the detecting difference of type such as A, G, four kinds of nucleic acids of C, T, need fluorescent dye is planted in test piece in advance, when the rayed of suitable wavelength during at sample, can produce different fluorescent brightness reactions because of the difference of DNA kind, judge that whereby whether nucleic acid exists.Because a little less than the fluorescence reaction, so the SNR (signal noise ratio) of fluorescent brightness reaction detection signal becomes extremely important.

See also Fig. 1, be the structural representation of known front projection biochip scanning apparatus, as shown in the figure: this biochip scanning apparatus 1 includes one can launch the generating laser 11 of laser light; One excites frequency spectrum filter disc (Excitation Spectral Filter) 12 in order to what filter the required excitation source wavelength accuracy of fluorescent dye; One one side can make light produce transmission, but the light splitting piece 13 of another side reflection ray; One can cross transmission the focusing objective len 14 of the light focusing of light splitting piece 13; The sampling bench 15 that examined samples 151 are arranged is put in one top, and sampling bench 15 can move freely in the X-Y direction, and can scan as single-point on sampling sample 151 via the light that focusing objective len 14 is focused on; One in order to filter the fluorescent emission frequency spectrum filter disc (Fluorescent Emission Spectral Filter) 16 of the fluorescence signal that examined samples 151 are excited after scanning; The one inductor imaging lens 17 that the fluorescence signal by fluorescent emission frequency spectrum filter disc 16 can be focused on; And a signal inducing receiver 18, can receive the fluorescence signal that focuses on by inductor imaging lens 17.When scanning, sampling bench 15 can move rapidly at X and Y direction, makes all examined samples 151 on it all can be scanned and analyze by single-point.So, though mobile sampling bench 15 so back and forth scan and can reach that single-point scans and the function analyzed will cause the cost of time and the vibration problem of system.

See also Fig. 2, be the structural representation of known rear-projection transmission-type biochip scanning apparatus, as shown in the figure:

The structure of this rear-projection transmission-type biochip scanning apparatus 2 is roughly the same with above-mentioned front projection biochip scanning apparatus 1 basically, only structurally light splitting piece 13 is removed.Effect and principle that its single-point scans are identical, but have the cost of time and the vibration problem of system equally.

See also Fig. 3, structural representation for another known front projection biochip scanning apparatus, as shown in the figure: the structure of this front projection biochip scanning apparatus 3 is roughly the same with above-mentioned front projection biochip scanning apparatus 1 basically, only being provided with one in reflecting

piece

13 and 14 of focusing objective lens can rotate in directions X, to change the rotating mirror 31 of light reflection angle.When scanning, can do the angle rotation by rotating mirror 31 and light can be scanned in the X axis sampling of sampling bench 15 to change the light reflection angle, so can save the mobile of X axis and scan, and can significantly reduce the harmful effect of platform vibration.

See also Fig. 4, structural representation for another known front projection biochip scanning apparatus, as shown in the figure: the structure of this front projection biochip scanning apparatus 4 is roughly the same with above-mentioned front projection biochip scanning apparatus 3 basically, only structurally rotary reflection polygonal mirror 31 is replaced as the reflective polygonal mirror 41 of a rotation, its effect and principle all with identical, can significantly reduce the harmful effect of platform vibration.

See also Fig. 5, structural representation for another known rear-projection transmission-type biochip scanning apparatus, as shown in the figure: the structure of this rear-projection transmission-type biochip scanning apparatus 5 is roughly the same with above-mentioned front projection biochip scanning apparatus 4 basically, only structurally change the rear-projection transmission-type into, in addition, signal inducing receiver 18 also can be used what device (CCD) of line style electric charge coupling.

So, though as using rotating mirror 31 among Fig. 3, Fig. 4 and Fig. 5 or rotating reflective polygonal mirror 41 and can save the mobile of X axis and scan, and can significantly reduce the harmful effect of platform vibration, but this mode is very easy to produce the turning axle beat because of the swing or the rotation of reflective mirror, cause radiation source can't focus on the sample, and can become ellipse because of anglec of rotation difference makes the light beam of irradiates light at the test piece edge to be out of shape, cause illumination not enough and uneven, make signal produce mistake.

The utility model content

Fundamental purpose of the present utility model promptly is to provide a kind of biochip scanning apparatus, can obtain the true signal noise ratio of calibration, to guarantee light signal correctness.

Another purpose of the present utility model promptly is to provide a kind of biochip scanning apparatus, can reach the effect that significantly improves scan speed.

Another purpose of the present utility model promptly is to provide a kind of biochip scanning apparatus, can reach the effect of minimizing time cost.

For achieving the above object, the Biological Crystal Plate Scanner that the utility model provides comprises:

One light source can be launched light;

One sampling bench, it is positioned at a side of this light source, and can put the sampling sample on this sampling bench, makes irradiate light this sampling sample on this sampling bench of this light source; And

One charge-coupled device (CCD), it is that this charge-coupled device (CCD) can receive this light that reflects via this sampling sample in this sampling bench top.

Wherein the light launched of this light source can be line style light.

Wherein a side of this light source is provided with the grating type hologram of a rotation, makes the grating type hologram of the light of this light source via this rotation, makes this light of incident produce diffraction and form diffraction light.

Wherein the kind of this light source can be laser, incandescence and gas excitation source one of them.

Wherein this sampling bench can move freely in the X-Y direction.

Wherein the light of this light source can be provided with one between this light source and this grating type hologram and excite the frequency spectrum filter disc via the grating type hologram of this rotation.

Wherein the light of this light source then can be provided with a focusing objective len via the grating type hologram of this rotation between this grating type hologram and this sampling bench.

Wherein the light of this light source then can be provided with a light splitting piece via the grating type hologram of this rotation between this grating type hologram and this sampling bench.

Wherein the light of this light source is via the grating type hologram of this rotation, and to form diffraction light, the diffraction rayed is on this sampling sample, and the light of this sampling sample reflection can be resent to this line style charge-coupled device (CCD) through a fluorescent emission frequency spectrum filter disc.

Wherein the light of this light source is via the grating type hologram of this rotation, and to form diffraction light, the light that this sampling sample reflects through the diffraction rayed can be resent to this line style charge-coupled device (CCD) through an inductor imaging lens.

Wherein the light launched of this light source is line style light, then can be provided with one between this light source and this sampling bench and excite the frequency spectrum filter disc.

Wherein the light launched of this light source is line style light, then can be provided with a light splitting piece between this light source and this sampling bench.

Wherein the light launched of this light source is line style light, then can be provided with a focusing objective len between this light source and this sampling bench.

Wherein the light launched of this light source is line style light, and this line style irradiate light is at this sample of taking a sample, and the light of this sampling sample reflection can be resent to this line style charge-coupled device (CCD) through a fluorescent emission frequency spectrum filter disc.

Wherein the light launched of this light source is line style light, this sampling sample meridional type irradiate light and the light that reflects can be resent to this line style charge-coupled device (CCD) through an inductor imaging lens.

Description of drawings

Fig. 1 is the structural representation of known front projection biochip scanning apparatus.

Fig. 2 is the structural representation of known rear-projection transmission-type biochip scanning apparatus.

Fig. 3 is the structural representation of another known front projection biochip scanning apparatus.

Fig. 4 is the structural representation of another known front projection biochip scanning apparatus.

Fig. 5 is the structural representation of another known rear-projection transmission-type biochip scanning apparatus.

Fig. 6 is an enforcement illustration of the present utility model.

Fig. 7 is that of the present utility model another implemented illustration.

Fig. 8 is minute surface and hologram beat comparison diagram.

Fig. 9 is an another enforcement illustration of the present utility model.

Figure 10 is an enforcement illustration more of the present utility model.

Specific implementation method

For further understanding the purpose of this utility model, feature and effect, by following specific embodiment, and conjunction with figs., the utility model is described in detail, illustrate as the back:

See also Fig. 6, be an enforcement illustration of the present utility model, as shown in the figure:

This front projection biochip scanning apparatus 6 comprises that one can launch the light source 61 of light, and this light source 61 can be the single-point light source, and its kind can be laser, incandescence and gas excitation source one of them; Side in light source 61 is provided with a rotatable grating type hologram 63, can make incident ray generation diffraction and form a diffraction light; In grating type hologram 63 1 ends one sampling bench 65 is set, put this sampling bench 65 tops sampling sample 651, and this sampling bench 65 can move freely in the X-Y direction, so that the sampling sample 651 on it can be done simple scan; And a line style charge-coupled device (CCD) (CCD) 68, can receive the light that reflects to taking a sample sample 651 by the diffraction rayed.In addition, can have additional one in this light source 61 and 63 of this grating type holograms and excite frequency spectrum filter disc 62, in order to the light source accuracy of the required excitation wavelength of filtration fluorescent dye; Can have additional a focusing objective len 64 and a light splitting piece 69 in this grating type hologram 63 and 65 of this sampling bench, this focusing objective len 64 can be crossed transmission the light focusing of grating type hologram 63, the one side of this light splitting piece 69 can make light produce transmission, another side can produce sampling sample 651 through irradiation fluorescence signal reflection; This sampling sample 651 is reflected by light splitting piece 69 through the fluorescence signal that irradiation produces, and can be through a fluorescent emission frequency spectrum filter disc 66, in order to filtering and sampling sample 651 after scanning, be excited the reflection the fluorescence signal, with an inductor imaging lens 67, fluorescence signal by fluorescent emission frequency spectrum filter disc 66 can be focused on, be resent to line style charge-coupled device (CCD) (CCD) 68.When scanning, the grating type hologram 63 of rotation with the laser beam of incident equably diffraction to sampling sample 651, and after seeing through focusing objective len 64 do corrections, can fully uniformly incident laser illumination be mapped on the sampling bench 65, so can obtain more correct signal noise ratio, guarantee the signal correctness.And the advantage of using the grating type hologram 63 of this rotation is significantly to eliminate the formed problem of turning axle beat, and being the LASER Light Source incident angle, condition precedent preferably is controlled at about 1 °～30 ° of Bragg regime, the diffraction angle will be equivalent to incident angle this moment, and turning axle beat in a small amount can be ignored, therefore the angular deviation that produces the time is about the per mille of using polygonal mirror or rotating mirror, but and can significantly promote the production demand of grating type hologram 63.

See also Fig. 7, for of the present utility model another implemented illustration, as shown in the figure:

The structure of this rear-projection penetration biochip scanning apparatus 7 is roughly the same with above-mentioned front projection biochip scanning apparatus 6 basically, only structurally changes the rear-projection transmission-type into, and light splitting piece 69 is removed.Its effect and principle all with identical, can obtain the true signal noise ratio of calibration, guarantee the signal correctness.

See also Fig. 8, be minute surface and hologram beat comparison diagram, as shown in the figure:

Left figure is general polygonal mirror turning axle deflection angle and the graph of a relation of reflection back angular deviation mistake among the figure, and when right figure is to use hologram among the figure with the deviation mistake graph of a relation of diffraction angle, wherein the longitudinal axis in left side tolerance is 1000 times that the right side longitudinal axis is measured.Therefore, can learn clearly that the angular deviation of using hologram to produce is about the per mille of using polygonal mirror or rotating mirror.

See also Fig. 9, be another enforcement illustration of the present utility model, as shown in the figure:

This front projection biochip scanning apparatus 8 includes and comprises that one can launch the line source 81 of light, and the kind of this line source 81 can be laser, incandescence and gas excitation source one of them; In a side of line source 81 one side is set and can makes light produce transmission, but the light splitting piece 83 of another side reflection ray; End in light splitting piece 83 is provided with a sampling bench 85, and put this sampling bench 85 tops sampling sample 851, and this sampling bench 85X-Y can move freely in the X-Y direction, so that the sampling sample 851 on it can be done simple scan; And a line style charge-coupled device (CCD) (CCD) 88, can receive by line source 81 and expose to sampling sample 851 and the light that reflects.In addition, can have additional one in this

line source

81 and 83 of this light splitting pieces and excite frequency spectrum filter disc 82, in order to the light source accuracy of the required excitation wavelength of filtration fluorescent dye; Can have additional a focusing objective len 84 in this

light splitting piece

83 and 85 of this sampling bench, it can will penetrate the light focusing of light splitting piece 83 and expose on the sampling sample 851; The fluorescence signal that this sampling sample 851 produces through irradiation penetrates injects a fluorescent emission frequency spectrum filter disc 86, in order to filtering and sampling sample 851 after scanning, be excited the reflection the fluorescence signal, with an inductor imaging lens 87, fluorescence signal by fluorescent emission frequency spectrum filter disc 86 can be focused on, be resent to line style charge-coupled device (CCD) (CCD) 88.

See also Figure 10, one implement illustration again for of the present utility model, as shown in the figure:

The structure of this rear-projection penetration biochip scanning apparatus 9 is roughly the same with above-mentioned front projection biochip scanning apparatus 8 basically, only structurally changes the rear-projection transmission-type into, and light splitting piece 83 is removed.

As Fig. 9 and shown in Figure 10, when scanning, utilize the luminescent method of line source form to shine the sampling sample that is scanned, make once to scan a little and increase, when utilizing the line style charge-coupled device (CCD) again, can accept signal exposure sampling simultaneously together, significantly improve scan speed, increase efficient and the cost of minimizing time.

The above is preferred embodiment of the present utility model only, is not in order to limit claim of the present utility model; All other do not break away from the equivalence of being finished under the spirit that the utility model discloses and changes or modify, and all should be included in the claim of application.

Claims

1, a kind of biochip scanning apparatus is characterized in that, comprising:

One light source is launched light;

One sampling bench is positioned at a side of this light source, and puts on this sampling bench and the sampling sample is arranged, the irradiate light of this light source this sampling sample on this sampling bench; And

One charge-coupled device (CCD), for above this sampling bench, this charge-coupled device (CCD) receives this light that reflects via this sampling sample.

2, biochip scanning apparatus as claimed in claim 1 is characterized in that, wherein the light launched of this light source is line style light.

3, biochip scanning apparatus as claimed in claim 1, it is characterized in that, wherein a side of this light source is provided with the grating type hologram of a rotation, and the light of this light source makes this light of incident produce diffraction and form diffraction light via the grating type hologram of this rotation.

4, biochip scanning apparatus as claimed in claim 1 is characterized in that, wherein the kind of this light source be laser, incandescence and gas excitation source one of them.

5, biochip scanning apparatus as claimed in claim 1 is characterized in that, wherein this sampling bench moves freely in the X-Y direction.

6, biochip scanning apparatus as claimed in claim 3 is characterized in that, wherein the light of this light source is provided with one between this light source and this grating type hologram and excites the frequency spectrum filter disc via the grating type hologram of this rotation.

7, biochip scanning apparatus as claimed in claim 3 is characterized in that, wherein the light of this light source is provided with a focusing objective len via the grating type hologram of this rotation between this grating type hologram and this sampling bench.

8, biochip scanning apparatus as claimed in claim 3 is characterized in that, wherein the light of this light source is provided with a light splitting piece via the grating type hologram of this rotation between this grating type hologram and this sampling bench.

9, biochip scanning apparatus as claimed in claim 3, it is characterized in that, wherein the light of this light source is via the grating type hologram of this rotation, form diffraction light, the diffraction rayed is on this sampling sample, and the light of this sampling sample reflection is resent to this line style charge-coupled device (CCD) through a fluorescent emission frequency spectrum filter disc.

10, biochip scanning apparatus as claimed in claim 3, it is characterized in that, wherein the light of this light source is via the grating type hologram of this rotation, form diffraction light, the light that this sampling sample reflects through the diffraction rayed is resent to this line style charge-coupled device (CCD) through an inductor imaging lens.

11, biochip scanning apparatus as claimed in claim 2 is characterized in that, wherein the light launched of this light source is line style light, is provided with one and excites the frequency spectrum filter disc between this light source and this sampling bench.

12, biochip scanning apparatus as claimed in claim 2 is characterized in that, wherein the light launched of this light source is line style light, is provided with a light splitting piece between this light source and this sampling bench.

13, biochip scanning apparatus as claimed in claim 2 is characterized in that, wherein the light launched of this light source is line style light, is provided with a focusing objective len between this light source and this sampling bench.

14, biochip scanning apparatus as claimed in claim 2, it is characterized in that, wherein the light launched of this light source is line style light, this line style irradiate light is at this sample of taking a sample, and the light of this sampling sample reflection is resent to this line style charge-coupled device (CCD) through a fluorescent emission frequency spectrum filter disc.

15, biochip scanning apparatus as claimed in claim 2, it is characterized in that, wherein the light launched of this light source is line style light, this sampling sample meridional type irradiate light and the light that reflects is resent to this line style charge-coupled device (CCD) through an inductor imaging lens.