CN2741052Y

CN2741052Y - Test paper for cow ovulation prediction

Info

Publication number: CN2741052Y
Application number: CN 200420084477
Authority: CN
Inventors: 万积成
Original assignee: Individual
Current assignee: Individual
Priority date: 2004-07-30
Filing date: 2004-07-30
Publication date: 2005-11-16
Anticipated expiration: 2014-07-30

Abstract

The utility model relates to a test paper for cow ovulation prediction which uses a colloidal gold immunity test paper to detect the LH content of a cow in ovulation, belonging to the field of colloidal gold immunoassay. The test paper for cow ovulation prediction comprising a bottom board, a water absorption board, a nitrocellulose film, an anti-LH single anti-gold mark pad and a glass fiber sample liquid absorption layer. When the serum and the milk fat of a cow contains LH antigen, the LH antigen in the sample can be bound specifically with an anti-LH beta gold mark probe and be recognized by an anti-LH alpha monoclonal antibody (MAb); double anti-sandwich specific binding is generated. The LH peak value of the cow in ovulation can be determined through the luteinizing hormone LH content of the serum and the milk fat of the cow in order to determine the corresponding ovulation period.

Description

The test paper that is used for the milk cow ovulation prediction

Technical field

The present invention relates to a kind of LH content of measuring the milk cow in oestrus with colloid gold immune test paper to carry out the test paper of milk cow ovulation prediction, belong to the colloid gold immune detection range.

Background technology

The national sales volume of milk is just with annual 30% speed increase, and the increment in milk source can only be limited to 10% limit increase.Under such imbalance between supply and demand situation, improve the breeding potential of milk cow, eugenic breeding, voluminous female calf has play a part crucial to the growth in milk source.Milk cow must just can give milk after calving, so the breeding potential of milk cow is directly connected to the level of giving milk.

Ovulation is the process that ovary discharges ovum, is the critical event in the ovarian cycle.Lutropin LH can promote the discharge of mature egg, before ovulation, LH value in blood and the butterfat can raise gradually, reach behind the peak value soon (12-23 hour) milk cow normal ovulation promptly takes place, thereby LH can be used as the accurate sign that advance notice is ovulated to time of peaking.The purpose of milk cow ovulation prediction is for the milk cow of ovulation is found out, and with timely breeding, improves conception rate, and can use X sperm, y sperm characteristic to do eugenic sex selection.Method commonly used mainly contains the visual observation method, examination per rectum method, ultrasonic diagnosis method and hormone detection method in the milk cow ovulation prediction at present.Wherein visual observation method is the more method of using at present, and it generally adopts the milk cow breeding in evening of accepting mounting the morning, accepts the milk cow of mounting afternoon, breeding the next morning.This method often can not accurately predicting milk cow ovulation period, reduces with the first feelings phase pregnancy rate that causes milk cow.The examination per rectum method requires the operator must have certain clinical experience, comparatively wastes time and energy, and also can cause disease propagation sometimes, and poor accuracy.Ultrasonic diagnosis will be subjected to the restriction of instrument and operative technique, and it can only confirm and can not predicting ovulation.And present hormone detection method also only is used for breadboard detection, and this is because its used technology---radioimmunology (RIA) and elisa immunoassay (EIA) all need be used specific installation and well-trained personnel, and operation is wasted time and energy.

The patent No. is a kind of eugenic test paper of 00222540.9, the patent No. is 00222539.5 1 kinds of infertile detection test paper, the present invention and above-mentioned two invention differences are, be coated with two lines on the nitrocellulose filter, article one, be LH α monoclonal antibody test wire, another is a sheep anti mouse polyclonal antibody control line, when collaurum is trapped in two places, the LH concentration that the detected value of response line is set at calver is greater than or equal to 10mIU/ml, the LH concentration that is response line representative calver when identical with the control line color or darker than control line color is more than the 10mIU/ml, and this test paper is used for the milk cow ovulation tests.

Summary of the invention

The present invention has overcome some shortcomings that exist in the prior art, provides a kind of and has detected the LH content of milk cow in oestrus by colloidal gold method, to carry out the test paper of milk cow ovulation prediction.

For solving the problems of the technologies described above, the present invention is achieved by the following technical solutions:

At milk cow ovulation prediction test paper base plate middle part is nitrocellulose filter, and a LH α monoclonal antibody test wire and a sheep anti mouse polyclonal antibody control line are arranged on the nitrocellulose filter, and the reacting value of test strips response line is set at more than or equal to 10mIU/ml; In base plate one end termination is water accepting layer, other end termination is the glass fiber sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with anti-LH β monoclonal antibody gold mark pad with water accepting layer respectively and are connected (the overlapping coupling part is in 1-2 millimeter scope), are pressed with the glass fiber sample liquid-adsorption layer on anti-LH β monoclonal antibody gold mark pad.Feature of the present invention is used for milk cow ovulation diagnosis, predicting ovulation time, fertilization in good time.

On the nitrocellulose filter of milk cow ovulation tests test paper the bag quilt be LH α monoclonal antibody, colloid gold label be LH β monoclonal antibody; Be used for the prediction of the ovulation of milk cow, improve the first feelings phase pregnancy rate.Utilize the apoptosis principle of milk cow X, Y chromosome sperm, can carry out the eugenic breeding of milk cow and reach sex controlling, voluminous female calf is a purpose.

Combined method about base plate, nitrocellulose filter, thieving paper, collaurum mark pad has been known technology.Just obtaining traditional Chinese medicines word authentication code (95) defends the accurate word of medicine (Jing Wanhua) S-02 number in nineteen ninety-five in Wanhuapuman Bioengineering Co Ltd, and with launch products.

Test shows: the LH hormone secretion amount in the milk cow body is during less than 10mIU/ml, and milk cow can not produce ovulation, so the reacting value of this test strips response line is set at more than or equal to 10mIU/ml.

The sample layer of milk cow ovulation prediction test paper is put into the serum of milk cow in oestrus, in the butterfat (liquid level must not surpass the MAX line), because the capillarity sample will move to the water accepting layer end along test strips, in the sample corresponding LH antigen can with anti-LH β gold mark probe generation specific bond after and by anti-LH alpha monoclonal antibodies identification, the specific bond of double antibodies sandwich promptly takes place, thereby by the serum of surveying, the content of interstitialcellstimulating hormone (ICSH) LH in the butterfat can be measured cow oestrus phase LH peak value, to determine its corresponding onset of ovulation, the gold mark probe that promptly is stranded in this zone will show corresponding redness, and the depth of this test wire color just is directly proportional with LH hormone-content in the institute test sample product.

Use detection paper to enter serum, the butterfat of milk cow in oestrus, when or response line identical with the control line color is darker than control line color as response line, show to occur the LH peak, spontaneous ovulation in 12-23 hour; Response line is more of light color or a control line only occurs and show no LH peak than control line.When moving to anti-mouse polyclonal antibody control line, no matter in the sample LH content what, gold mark probe all can combine delay with the mouse IgG of bag quilt on the nitrocellulose filter, it is red that control line is shown.So control line does not have, and colour band produces then representative operation wrong (during detection, the sample liquid level surpasses the MAX line) or test paper is expired.

Because adopt technique scheme, the ovulation diagnostic method of milk cow provided by the present invention has such beneficial effect: i.e. high specificity, highly sensitive, easily store, need not the technical skill personnel operation, do not need instrument and equipment, and readability as a result.

Description of drawings

Fig. 1 is the main TV structure figure of milk cow ovulation prediction test paper.

Fig. 2 is the sectional structure chart of overlooking of figure one.

Fig. 3 is shown as positive findings figure when being used to test for milk cow ovulation prediction test paper.

Fig. 4 is shown as negative findings figure when being used to test for milk cow ovulation prediction test paper.

Specific embodiment

It is the optimum detection time that detection began to stop back 48 hours to oestrus from the cow oestrus phase.Remove external packing during use, take out test strips, test strips sample end is immersed serum, in the butterfat dilution (liquid level must not surpass the MAX line), because the capillarity sample will move to the water accepting layer end along test strips, work as serum, when having LH antigen in the butterfat, in the sample LH antigen can with anti-LH β gold mark probe generation specific bond after and by anti-LH alpha monoclonal antibodies identification, the specific bond of double antibodies sandwich promptly takes place, thereby can measure cow oestrus phase LH peak value by the content of the interstitialcellstimulating hormone (ICSH) LH in the butterfat, to determine its corresponding onset of ovulation, the gold mark probe that promptly is stranded in this zone will show corresponding redness, and the depth of this response line color just is directly proportional with LH hormone-content in the butterfat; The LH concentration of representative calver was more than the 10mIU/ml when response line was identical with the control line color or darker than control line color.

The result judges: as two red line occurring at detection zone and control zone, then positive when the identical or test wire color of color is deeper than control line; It is negative when test wire is shallower than control line or a control line only occurs; Red line does not appear in the control zone, shows the expired or misoperation of test paper.

Claims

1. one kind is used for milk cow ovulation prediction test paper, it is characterized in that: it is by base plate (7), water sucting plate (1), nitrocellulose filter (2), anti-LH monoclonal antibody gold mark pad (5), glass fiber sample liquid-adsorption layer (6) is formed, the base plate middle part is a nitrocellulose filter, a LH α monoclonal antibody test wire (4) and a sheep anti mouse polyclonal antibody control line (3) are arranged on the nitrocellulose filter, in base plate one end termination is water sucting plate, other end termination is the glass fiber sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with anti-LH monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the glass fiber sample liquid-adsorption layer on anti-LH monoclonal antibody gold mark pad.

2. test paper according to claim 1 is characterized in that: the LH concentration that the detected value of response line is set at calver is greater than or equal to 10mIU/ml.