CN2581064Y - Thermostat perfusion apparatus for isolated organ - Google Patents
Thermostat perfusion apparatus for isolated organ Download PDFInfo
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- CN2581064Y CN2581064Y CN 02280295 CN02280295U CN2581064Y CN 2581064 Y CN2581064 Y CN 2581064Y CN 02280295 CN02280295 CN 02280295 CN 02280295 U CN02280295 U CN 02280295U CN 2581064 Y CN2581064 Y CN 2581064Y
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- temperature
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- constant temperature
- air pump
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Abstract
The utility model discloses a constant temperature perfusion apparatus for an isolated organ, which is composed of a temperature controlling part, a constant temperature part, an oxygen supply sample application part, a transfusion part, a sample tank, and a base, wherein, the temperature controlling part comprises a temperature controlling casing, the inner part of which is provided with a temperature controlling circuit and an air pump, and the temperature controlling casing is provided with a power supply switch, an air pump switch, a temperature regulating knob, a temperature setting switch, a probe changeover switch, and a liquid crystal display. The constant temperature part comprises a constant temperature tank, the inner part is provided with a heating tube and a temperature sensing probe, the oxygen supply sample application part is arranged above the constant temperature tank, and the inner part of the sample tank is provided with a temperature sensing probe. The temperature controlling circuit is composed of a digital thermometer module having a temperature measuring function, a temperature display function, and a temperature alarm function, a triode, and a relay. The sample tank of the utility model is a horizontal tank, the periphery is provided with a sample outer tank, and a transfusion device can adopts an ordinary transfusion bottle or a Mariotte container. The utility model has the advantages of stably, simpleness, and strong practicability and has multiple functions such as constant temperature, constant pressure, constant flow, oxygen supply, display, stir, etc. The utility model is suitable for animal isolated organs of the physiological subject.
Description
Technical field
The utility model relates to a kind of experiment special equipment, relates in particular to a kind of isolated organ constant temperature perfusion instrument.
Background technology
Isolated organ perfusion device in the past be classical " Biao Shi bath " promptly with alcolhol burner heating, water temperature is raise heat is reached glass inside groove (being the Maxwell groove) again and heats up and reach and do experiment purpose.Along with the hyundai electronics development of science and technology, had automatic thermostatic device, so-called Magnus' bath thermostat also emerges.But, in the certain temperature environment of need maintenance, also need provide oxygen to the requirement of experiment of isolated organ, in experimentation, need the perfusion flushing after the dosing reaction, whole process still need keep temperature constant state, but also must keep the continuity of the primitive curve of sample reaction not to be destroyed.Increased difficulty to experimental provision like this, common product is difficult to reach requirement, even some step also needs manual operations flushing sample.Its complex operation has influenced experimental result again.
Summary of the invention
The purpose of this utility model is: overcome above-mentioned technological deficiency, a kind of stable, easy, practical isolated organ constant temperature perfusion instrument is provided.
The utility model is realized by following scheme: be made up of temperature control portion, constant temperature portion, logical oxygen application of sample portion, transfusion portion, sample groove.Temperature control portion comprises the temperature control shell, in establish temperature-adjusting circuit and air pump, power switch, air pump switch, temperature adjustment knob, temperature are set on the temperature control shell establish switch, probe change-over switch, LCDs.Constant temperature portion comprises thermostat, in establish heating tube and temperature-sensing probe, heating tube top is provided with the heat radiation window, on the heat radiation window mozzle is arranged surely, mozzle one termination infusion set, an end of another termination multi-orifice valve, the multi-orifice valve other end is connected to the sample groove.The thermostat top is provided with logical oxygen application of sample portion, comprise a pair of triple valve and multi-orifice valve, the air pump efferent duct is connected to sample groove and thermostat again by triple valve, be provided with temperature-sensing probe in the sample groove, temperature detection, temperature show temperature-adjusting circuit, the digital thermometer module of temperature alarming function by having, and triode, relay constitute.Digital thermometer module controls output pin is connected to transistor base, the relay cross-over connection is in transistor collector and dc source anode, get dead electricity in order to control it, its normally-closed contact and heating tube and power switch constitute the branch road of connecting, and this branch road and another are connected between AC power by the series arm that air pump M and air pump switch constitute.The probe change-over switch is a single-pole double-throw switch (SPDT), in order to control the temperature-sensing probe work of selecting to be built in the digital thermometer module.
The also configurable base of the utility model, and be furnished with transfusion pole thereon and in order to support the fixedly pole of transducer.
Sample groove of the present utility model is horizontal groove, and outer periphery is equipped with the sample water jacket, and infusion set can adopt common transfusion bottle or Ma Liaoteshi container.
The utility model constant temperature adopts two probe thermometrics, comprises the temperature of water jacket and inside groove, and instrument is in the past just surveyed the water jacket temperature, is difficult to like this guarantee whether the temperature of inside groove reaches desirable value.Common transfusion device or Ma Liaoteshi case are adopted in constant current, can keep constant pressure and flow, needing after the dosing in the experiment to have avoided the complicated procedures of repeatedly manual perfusion flushing, do not influence experiment continue carry out.Stirring is to stir by gas, impels the water jacket water temperature all permanent rapidly.Logical oxygen adopts triple valve control, and the big I of air-flow is regulated voluntarily.Instrument is furnished with digital lcd and shows that the experimenter can intuitively read temperature, grasps variations in temperature.Horizontal sample groove has changed traditional vertical groove, is more conducive to fix, guides and observes.The utility model is stable, easy, practical, and has multifunctionalities such as constant temperature, constant voltage, constant current, logical oxygen, demonstration, stirring, is applicable to the experiment of physiology subject animal isolated organ.
Description of drawings
Fig. 1 is the utility model structural representation;
Fig. 2 is the circuit diagram of the utility model temperature-adjusting circuit.
The specific embodiment
Be elaborated below in conjunction with embodiment of the present utility model.
Present embodiment is made up of temperature control portion, constant temperature portion, logical oxygen application of sample portion 14, transfusion portion, sample groove 20 and base 23.Being furnished with transfusion pole 24 on the base 23 reaches in order to support the fixedly pole 25 of transducer, the upper right side is provided with temperature control portion, temperature control portion comprise shell 1, in establish temperature-adjusting circuit and air pump M, power switch 3, air pump switch 2, temperature adjustment knob 4, temperature are set on the temperature control shell 1 establish switch 5, probe change-over switch 7, LCDs 6 and thermostatical pilot lamp 8.Be arranged at the upper left constant temperature of base 23 portion and comprise thermostat 9, in establish heating tube 10 and temperature-sensing probe 13, heating tube 10 tops are provided with heat radiation window 11, be fixed with the snakelike mozzle of making by stainless steel 12 on the heat radiation window 11, mozzle 12 1 termination infusion sets 21, one end of another termination multi-orifice valve 17, multi-orifice valve 17 other ends are connected to the sample groove 20 that is arranged at temperature control portion top.Top, thermostat 9 top is provided with logical oxygen application of sample portion 14, comprises a pair of triple valve 16 and multi-orifice valve 17, and air pump M efferent duct is connected to sample groove 20 and thermostat 9 through triple valve 16.Sample groove 20 outer periphery are equipped with sample water jacket 18, be provided with temperature-sensing probe 19 in the sample groove 20, temperature-adjusting circuit is by having temperature detection, temperature shows, the digital thermometer module I C of temperature alarming function, triode BG, relay J constitutes, digital thermometer module I C control output end pin M1 is connected to triode BG base stage, the relay J cross-over connection is in triode BG colelctor electrode and dc source anode, get dead electricity in order to control it, its normally-closed contact and heating tube 10 and power switch 3 constitute the branch road of connecting, this branch road and another are connected between AC power by the series arm that air pump M and air pump switch 2 constitute, the normally opened contact of relay J and thermostatical pilot lamp 8 constitute series arm and also are connected between AC power, probe change-over switch 7 is a single-pole double-throw switch (SPDT), temperature- sensing probe 13,19 are built in the digital thermometer module I C, the outer end all is connected to probe change-over switch 7 switch terminal, and probe change-over switch 7 is termination digital thermometer module I C temperature-sensitive input fixedly.
The course of work of the present utility model and operation principle are: with digital thermometer module I C is core, the perfusion instrument temperature-control circuit course of work is as follows: I) 7 probe change-over switches are beaten downwards (a point and c point are connected), promptly use the temperature that thermostat 9 was surveyed and shown to temperature-sensing probe 13 earlier; II) press temperature and establish switch 5, IC will enter temperature state will be set; At this moment, press adjustment button 4, the temperature alarming value can be set; Temperature has been set, and establishes switch 5 by temperature again and makes IC return to the temperature detection state; III) the power turn-on switch 3, and heating tube 10 forms the loop by the normally-closed contact of relay J, the liquid in the beginning heated constant temperature groove 9; IV) when the near design temperature of fluid temperature in the thermostat 9, with the switch opens of infusion set 21, the mozzle 12 of flow of solution through being arranged in thermostat 9 of experiment usefulness is heated, and flows at last in the sample groove 20 by manually; Simultaneously, by 7 dozens of the change-over switches (a point and b point are connected) upward of manually will popping one's head in, promptly use the temperature that sample groove 20 was surveyed and shown to temperature-sensing probe 19; V) when the temperature of solution in the sample groove 20 reaches the temperature alarming value of setting, the pin M1 of IC will produce a high level signal, and this signal is delivered to transistor BG base stage; Triode BG conducting, the relay J coil gets electric, and the normally-closed contact of relay J disconnects immediately, and heating tube 10 disconnects with power supply and stops to heat; The normally opened contact closure of relay J is lighted thermostatical pilot lamp 8 simultaneously; VI) the air pump switch 2 that closes, air pump M motor gets electric, is the logical oxygen of solution of sample groove 20; And be the liquid aerating stirring in the thermostat, make its temperature equalization; VII) when the temperature of solution in the sample groove 20 was lower than the temperature alarming value of setting, IC pin M1 dropped to low level by high level immediately, the coil losing electricity of relay J, the normally-closed contact closure of relay J immediately, heating tube 10 electricly begin heating again; The normally opened contact of relay J disconnects simultaneously, and thermostatical pilot lamp 8 extinguishes; VIII) repeat V) to VII) job step, can make the temperature of solution in the sample groove 20 maintain the temperature value of setting all the time to satisfy experimental requirements.
Claims (7)
1, a kind of isolated organ constant temperature perfusion instrument, it is characterized in that: by temperature control portion, constant temperature portion, logical oxygen application of sample portion (14), transfusion portion, sample groove (20) is formed, temperature control portion comprises temperature control shell (1), in establish temperature-adjusting circuit and air pump (M), power switch (3) is set on the temperature control shell (1), air pump switch (2), temperature adjustment knob (4), temperature is established switch (5), probe change-over switch (7), LCDs (6), constant temperature portion comprises thermostat (9), in establish heating tube (10) and temperature-sensing probe (13), heating tube (10) top is provided with heat radiation window (11), be fixed with mozzle (12) on the heat radiation window (11), mozzle (12) one termination infusion sets (21), one end of another termination multi-orifice valve (17), multi-orifice valve (17) other end is connected to sample groove (20), thermostat (9) top is provided with logical oxygen application of sample portion (14), comprise a pair of triple valve (16) and multi-orifice valve (17), air pump (M) efferent duct is connected to sample groove (20) and thermostat (9) by triple valve (16), be provided with temperature-sensing probe (19) in the sample groove (20), temperature-adjusting circuit is by having temperature detection, temperature shows, the digital thermometer module (IC) of temperature alarming function, triode (BG), relay (J) constitutes, digital thermometer module (IC) control output end pin M1 is connected to triode (BG) base stage, relay (J) cross-over connection is in triode (BG) colelctor electrode and dc source anode, get dead electricity in order to control it, its normally-closed contact and heating tube (10) and power switch (3) constitute series arm, this branch road and another are connected between AC power by the series arm that air pump (M) and air pump switch (2) constitute, probe change-over switch (7) is a single-pole double-throw switch (SPDT), temperature-sensing probe (13,19) be built in the digital thermometer module I C, the outer end all is connected to probe change-over switch (7) switch terminal, and probe change-over switch (7) is termination digital thermometer module (IC) temperature-sensitive input fixedly.
2, isolated organ constant temperature perfusion instrument according to claim 1 is characterized in that: dispose base (23), temperature control portion, constant temperature portion, logical oxygen application of sample portion (14), sample groove (20) all are arranged at its top.
3, isolated organ constant temperature perfusion instrument according to claim 1 and 2 is characterized in that: be furnished with transfusion pole (24) on the base (23) and reach in order to support the fixedly pole (25) of transducer.
4, isolated organ constant temperature perfusion instrument according to claim 1 and 2, it is characterized in that: sample groove (20) is horizontal groove.
5, isolated organ constant temperature perfusion instrument according to claim 1 and 2, it is characterized in that: infusion set (21) can adopt common transfusion bottle or Ma Liaoteshi container.
6, isolated organ constant temperature perfusion instrument according to claim 1 and 2, it is characterized in that: sample groove (20) outer periphery is equipped with sample water jacket (18).
7, isolated organ constant temperature perfusion instrument according to claim 1 and 2, it is characterized in that: temperature control shell 1 is provided with thermostatical pilot lamp (8), and the normally opened contact of relay (J) and thermostatical pilot lamp (8) constitute series arm and also are connected between AC power.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02280295 CN2581064Y (en) | 2002-12-02 | 2002-12-02 | Thermostat perfusion apparatus for isolated organ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02280295 CN2581064Y (en) | 2002-12-02 | 2002-12-02 | Thermostat perfusion apparatus for isolated organ |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2581064Y true CN2581064Y (en) | 2003-10-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02280295 Expired - Fee Related CN2581064Y (en) | 2002-12-02 | 2002-12-02 | Thermostat perfusion apparatus for isolated organ |
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| Country | Link |
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| CN (1) | CN2581064Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102813559A (en) * | 2012-08-27 | 2012-12-12 | 山东大学 | Full-automatic hemoperfusion device |
| CN103920401A (en) * | 2014-04-25 | 2014-07-16 | 滨州医学院附属医院 | Thermal insulation device for water-bath heating and dissolving of mannitol |
| CN104897886A (en) * | 2015-05-27 | 2015-09-09 | 广东省中医院 | Constant-temperature incubator for isolated perfused heart |
| CN108717112A (en) * | 2018-06-08 | 2018-10-30 | 新乡医学院 | A kind of Perfused isolated heart device suitable for rat |
| CN108917167A (en) * | 2018-08-07 | 2018-11-30 | 江苏斯托利仪器仪表有限公司 | A kind of constant temperature perfusion system |
-
2002
- 2002-12-02 CN CN 02280295 patent/CN2581064Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102813559A (en) * | 2012-08-27 | 2012-12-12 | 山东大学 | Full-automatic hemoperfusion device |
| CN103920401A (en) * | 2014-04-25 | 2014-07-16 | 滨州医学院附属医院 | Thermal insulation device for water-bath heating and dissolving of mannitol |
| CN103920401B (en) * | 2014-04-25 | 2015-08-05 | 滨州医学院附属医院 | A kind of sweet mellow wine heating water bath dissolves attemperator |
| CN104897886A (en) * | 2015-05-27 | 2015-09-09 | 广东省中医院 | Constant-temperature incubator for isolated perfused heart |
| CN104897886B (en) * | 2015-05-27 | 2017-02-22 | 广东省中医院 | Constant-temperature incubator for isolated perfused heart |
| CN108717112A (en) * | 2018-06-08 | 2018-10-30 | 新乡医学院 | A kind of Perfused isolated heart device suitable for rat |
| CN108717112B (en) * | 2018-06-08 | 2019-08-30 | 新乡医学院 | A kind of Perfused isolated heart device suitable for rat |
| CN108917167A (en) * | 2018-08-07 | 2018-11-30 | 江苏斯托利仪器仪表有限公司 | A kind of constant temperature perfusion system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20031022 Termination date: 20100104 |