CN2578831Y

CN2578831Y - Antibody inspection reagent box of virus of infectious fowl bronchitis

Info

Publication number: CN2578831Y
Application number: CN 02285790
Authority: CN
Inventors: 陈福勇; 王宏俊; 曹红
Original assignee: 陈福勇
Priority date: 2002-11-21
Filing date: 2002-11-21
Publication date: 2003-10-08
Anticipated expiration: 2012-11-21

Abstract

The utility model relates to a virus antibody detecting reagent box for fowl infectious bronchitis. Positive blood serum, negative blood serum, an enzyme mark second antibody, substrate concentrated solution, substrate dilute solution, blood serum dilute solution, scrubbing solution and stopping solution are arranged in a box body, and in addition, a sealed covering plate is also arranged in the box body, and the use method of the utility model can be seen from a concrete implementation mode. The utility model can be used for effectively detecting the antibody level of a fowl and knowing an immune effect, has the advantages of sensitive response, high specificity and simple operation and is suitable for all levels of veterinary medicine prevention organizations, scientific research teaching institutions, animal biologic product factories and large and medium sized breeding enterprises.

Description

The avian infectious bronchitis virus antibody assay kit

Technical field:

The utility model relates to technical field of measurement and test, particularly the test of bird antibody.

Background technology:

Know kind surplus the avian infectious bronchitis virus (coronavirus) that works the mischief in causing producing has 20 at present.Tens of kinds of the vaccines that is used to prevent, whether each is variant for immune programme for children, immune time, the specific aim of vaccine, have cross protection very not identical.At the problems referred to above, how to solve, the monitoring behind the vaccine immunity is particularly important.Fast detecting by the specific antibody diagnostic kit; grasp the different maternal antibody growth and decline of chick rule; mensuration to immune efficacy after the conventional vaccine immunity; assessment to the effectiveness behind the different specific aim vaccine immunities of the biological producer of difference; grope the relation between group specificity and the type specificity antibody; find out the rule of protection antibody generation and the characteristics of wild virus infection, on this research basis, formulate the immune programme for children that reasonably is fit to China's national situation.The sick detection of antibodies of the infective bronchitis of present domestic application still rests on some traditional diagnostic methods, doubts certain methods such as inhibition such as AGP, blood.All there are some problems in these methods on susceptibility, specificity.

Summary of the invention:

The purpose of this utility model is to overcome the deficiency that above-mentioned known technology exists, thereby a kind of avian infectious bronchitis virus antibody assay kit is provided, and can detect antibody horizontal effectively, understands immune effect.

The purpose of this utility model realizes in the following way:

This kit has a box body (10), wherein: have positive serum (1), negative serum (2), ELIAS secondary antibody (3), substrate concentrate (4), substrate dilution (5), serum dilution (6), cleansing solution (7) and stop buffer (8) in the box body (10), above-mentioned various liquid is contained in the bottle respectively, in addition, also have the bag of aluminum plastic film bag encapsulation in the box body (10) by plate (9).

The utility model usable range is as follows:

Clone, order-checking, the sequential analysis of chicken sensitivities bronchitis virus nucleoprotein gene.

The mensuration of the protein expression of chicken sensitivities bronchitis virus nucleoprotein gene, purifying, protein concentration.

The ELISA bag is developed, is wrapped by the mensuration of time by the comparison of the mensuration of avian infectious bronchitis virus nucleoprotein concentration, coating buffer, confining liquid.

Bag is by the comparison of the optimum concentration of concentration and antibodies mensuration, antibody diluent, the Best Times of antigen-antibody reaction.

The best effort concentration of the preparation of ELIAS secondary antibody, ELIAS secondary antibody, the comparison of ELIAS secondary antibody stabilizing agent, the action time of ELIAS secondary antibody.

The stability of the selection of substrate, the time of substrate-function, substrate.

The selection of stop buffer and preparation.

The mensuration of various composition storage lives, assembling kit storage life are measured.

The check of kit specificity, susceptibility are checked, are compared test with external kit.

Blocking experiment.

The formulation of positive serum typical curve.

The utility model compared with prior art has following advantage:

Use the utility model can detect the level of cockscomb shape virus groups specific antibody, thereby understand the immune effect of chicken.

This kit is simple to operate, has characteristics such as quick, responsive, special, stable.Can be widely used in the detection of infectious bronchitis of chicken serum antibody, have a good application prospect.

Description of drawings:

Fig. 1 is the utility model front view;

Fig. 2 is the utility model vertical view.

In Fig. 2: (1) positive serum; (2) negative serum; (3) ELIAS secondary antibody; (4) substrate concentrate; (5) substrate dilution; (6) serum dilution; (7) cleansing solution; (8) stop buffer; (9) bag is by plate; (10) box body.

Embodiment:

Step is as follows during use:

1, adds the positive and negative control serum: in two holes, add liquid respectively No. 1, No. 2, every hole 100 microlitres.

2, add sample: after serum to be checked is pressed 1: 100 dilution proportion with No. 6 liquid, add bag by (every hole adds 100 microlitres) in the hole of plate, 37 ℃ were reacted 30 minutes.

3, washing: get rid of liquid No. 6, add liquid 200 microlitres, continuous washing three times No. 7 to every hole.

4, add ELIAS secondary antibody: every hole adds No. 3 liquid 100 microlitres, 37C reaction 30 minutes.

5, washing: get rid of No. 3 liquid and add No. 7 liquid 200 microlitres, continuous washing three times to every hole.

6, add substrate: get rid of liquid No. 7, with after No. 5 liquid mixed by 1: 1, every hole adds 100 microlitres, reacts to add the every hole of liquid 100 microlitres after 10 minutes No. 8 with No. 4 liquid.

7, reading: microplate reader is selected the 665nm wavelength readings, and 490nm is long with reference to glass, the OD value greater than 0.14 promptly positive.

Remarks: not the time spent, be stored in 4 ℃ of environment.

Claims

1, a kind of avian infectious bronchitis virus antibody assay kit, have a box body (10), it is characterized in that: have positive serum (1), negative serum (2), ELIAS secondary antibody (3), substrate concentrate (4), substrate dilution (5), serum dilution (6), cleansing solution (7) and stop buffer (8) in the box body (10), above-mentioned various liquid is contained in the bottle respectively, in addition, also have the bag of polybag encapsulation in the box body (10) by plate (9).

2, kit according to claim 1 is characterized in that: box body (10) is set up kit and the criterion that a kind of indirect enzyme-linked immunosorbent assay method detects IBV antibody in the chicken serum.

3, kit according to claim 1 is characterized in that: above-mentioned antigen coated microplate (9), and use and express nucleoprotein bag quilt, be used to detect infectious bronchitis of chicken group specificity antibody.