CN2574187Y - Ice forceps for operating microbody - Google Patents
Ice forceps for operating microbody Download PDFInfo
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- CN2574187Y CN2574187Y CN02257290U CN02257290U CN2574187Y CN 2574187 Y CN2574187 Y CN 2574187Y CN 02257290 U CN02257290 U CN 02257290U CN 02257290 U CN02257290 U CN 02257290U CN 2574187 Y CN2574187 Y CN 2574187Y
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- ice
- tweezer
- probe
- small items
- handle small
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Abstract
The utility model relates to a pair of ice forceps for operating tiny objects, which comprises a semiconductor refrigerator, a tiny probe, one end of which is contacted with the cold end of the semiconductor refrigerator, and the other end of the tiny probe is fixedly connected with an ice forceps probe, a miniature electrical heating film which is coated on the outer surfaces of the tiny probe and the inc forceps probe, wherein, the semiconductor refrigerator is composed of a semiconductor refrigeration sheet, a radiating fin and a power source, wherein, the power source is electrically connected with the semiconductor refrigeration sheet by a conducting wire. The cylindrical tiny probe and the conical ice forceps probe, the conical degree of which is from 1 DEG to 10 DEC, form an integral structure. Because the ice forceps for operating tiny objects adopts semiconductor refrigeration, different heating can be realized by changing the size of the current of the miniature electrical heating film, and thereby, the operation and the unloading of tiny objects can be realized by controlling the size of an ice ball. The utility model has the advantages of good operating continuity, wide controllable displacement range, fast speed response, large force, simple structure, low cost, simple operation, etc.
Description
Technical field
The utility model relates to a kind of operating means to having moisture or placing the small items of suspension liquid to handle, and the ice tweezer that has moisture or place the small items of suspension liquid is fixed and handled to particularly a kind of ice hockey that forms when utilizing liquid freezing.
Background technology
An important trend of current natural science and engineering development is to stride forward towards microminiaturization, wherein the manipulation to small items is a big class major issue, such as under many micro-/ nano device application occasions and processing situation, need realize accurate displacement control to small items; In the recent period, people have proposed in liquid environment small items to be carried out Laser Processing, and this also depends on microoperation; Particularly, in the Medical Biology field, small cell body is carried out active operation to be dreamed of by the cell biological scholar for a long time, saying [Li Yinmei compiling according to Bell laboratory scientist Ashkin (proposition optical tweezer technology in 1986), light tweezer principle, technology and application, Hefei: China Science ﹠ Technology University, 1996], ", opened the gate of accurate research cell function for us " with the ability that organelle is removed from its normal position.
At present, small items in handling liquid environment such as cell etc., the light tweezer is one of the most promising technology, this is the method that a kind of optical pressure that causes based on the laser optics effect is handled small items, it makes and can draw and handle [Li Yinmei compiling to the molecule that falls into the formed light field of light of light tweezer, light tweezer principle, technology and application, Hefei: China Science ﹠ Technology University, 1996], thereby can catch and transport chromosome rapidly thus, bacterium, virus even cell, this technology are that cell imports foreign gene, research and change chromosome structure, the structure function of research organelle and carry out life science advanced subject such as species hybridization and showed wide prospect.Yet also there is certain deficiency in optical tweezer technology, and this is mainly reflected in, and 1. unmanageable laser energy may cause the temperature rise of institute's manipulated cell too high down to the destruction cell, thereby active somatic cell research is interrupted; And, the cover laser optical tweezer system that realizes needs laser instrument and regulating device thereof, during using, optical tweezer technology to light beam be gathered the luminous point of sub-micron by microscope, be aided with precision optical machinery positioning system, optical microscope and other technology again and accurately locate, handle and observe cell, make that thus 2. sleeve mechanism is huge, the equipment complexity, the expense costliness; Moreover 3. for some volumes and even the bigger small items such as cell of quality, the intensity of pressure of laser light is not sufficient to cause it to be subjected to displacement, thereby can make operation invalid.
Except that optical tweezer technology, people also attempt adopting other modes to control small items as mechanism of action such as electricity, magnetic, sound, but these modes all will be introduced outer field effect bar none, thereby can change some physical chemistry behavior of object itself, so that influence is to the understanding of actual physical process.
At present, it is found that thermal signal is one of control " molecule " only instrument [Liu Jing, micrometer/nanometer yardstick thermal conduction study, Beijing: Science Press, 2001].Compare with the heat driving, some traditional little actuation technology such as heap type piezoelectric actuator are if will realize bigger power output, then its displacement less relatively (＜10 μ m); Though and bimorph can obtain big displacement, power output is less relatively again; In addition, driven by power is then very responsive to particle and wet environment; Be difficult for being worked into the submillimeter yardstick and mangneto is moving.Based on above consideration, the utility model utilizes the special performance of by the use of thermal means, provide a kind of and can fix and handle the especially device of the small items in the suspending liquid environment such as cell etc. of small items, overcome the deficiency of microoperation devices such as above-mentioned smooth tweezer to a certain extent, become a kind of brand-new safely and effectively means of handling small items.
Summary of the invention
The purpose of this utility model is to provide a kind of ice hockey that generates when utilizing liquid freezing to reach fixing and handle the particularly ice tweezer that is used to handle small items of biological cell of small items.
The technical solution of the utility model:
The ice tweezer that is used to handle small items that the utility model provides is characterized in that, this ice tweezer comprises:
Semiconductor refrigerator 5,
One fine probe 1, an end of this fine probe 1 contacts with semiconductor cooler 5 cold junctions, the fixedly connected at least one ice tweezer probe 2 of the other end, and
One miniature electrical-heating film 3, this miniature electrical-heating film 3 are coated on the part or all of outside surface of fine probe 1 and ice tweezer probe 2;
Described semiconductor cooler 5 is made up of semiconductor cooling piece 51, one heat radiator 52 and a power supply 53, and power supply 53 is electrically connected with semiconductor chilling plate 51 and heat radiator 52 by lead 54; Described semiconductor chilling plate is a cube, and its length is of a size of 1-50mm * 1-50mm * 0.1-50mm;
Described fine probe 1 is a right cylinder, and its diameter is 1mm-5mm, and length is 5mm-50mm, and described ice tweezer probe 2 is the cone into a single integrated structure with the fine probe of this cylinder type 1, and the cone tapering is 1-10 °:
The film that described miniature electrical-heating film 3 constitutes for the electrical heating wire that is coated with adiabatic membrane on a kind of its outside surface, described electrical heating wire is formed by nickel-chromium alloy filament winding system, and described adiabatic membrane is a poly tetrafluoroethylene; Described miniature electrical-heating film 3 thickness are 1nm-1mm;
Described fine probe 1 and ice tweezer probe 2 are made by high thermal conductivity material; Described high thermal conductivity material is a stainless steel, and copper is silver-colored or golden;
Described ice tweezer 2 outside surfaces of popping one's head in are provided with a temperature sensor 4; Described temperature sensor 4 is a thermocouple temperature sensor;
The ice tweezer that is used to handle small items that the utility model provides, also can further comprise an operating control device, this operating control device comprises that one is used for fixing described fine probe 1, and the microoperation platform 81 that it is moved as three dimensions, one is used to control the microoperation controller 82 of these microoperation platform 81 actions, and microoperation controller 82 is by a computer 83 control operations; Described operating control device also comprises a microscope 84 that is connected with computer 83.
In sum, the ice tweezer that is used to handle small items that the utility model provides, owing to adopt semiconductor refrigerating, realize different heating functions by changing the size of current of miniature electrical heating on thin, thereby realize the carrying and the unloading of small object, particularly with the size of controlling ice hockey, can realize operation and unloading to more same product, and its operating continuity is good, controlled displacement range is wide, speed responsive is fast, simple in structure, cost is low, operates advantages such as very easy.
In said apparatus,, make fine probe 1 especially ice tweezer probe 2 and freeze, thereby generate ice hockey in the tip that ice tweezer probe 2 contacts with liquid to predetermined temperature by starting semiconductor cooler 5; And, can control the size that generates ice hockey by changing the intensity of heating current in the miniature electrical-heating film; Further strengthen the temperature of heating current in the miniature electric heating film, can realize the unloading of small object; Ice the temperature on the tweezer probe 2 simultaneously, can be by this temperature sensor 4 monitorings and feedback of being fixed on this ice tweezer probe 2.
The ice tweezer that the utility model provides is fixed by small items and manipulation process is achieved in that
A). fine probe 1 is fixed on the microoperation platform 81, starts microoperation controller 82, computer 83 and microscope 84;
B). by the observation of microscope 84, make the tip of ice tweezer probe 2 come close to or in contact with the small items that places liquid or have moisture; Start semiconductor cooler 5, set the size of current in the miniature electrical heating thin 3 simultaneously; After several seconds, ice hockey promptly forms on the surface of small items towards ice tweezer tip, and then this small items is fixed;
C). in conjunction with microscope 84 and microoperation instrument 82, the small items that is fixed on ice tweezer probe tip is carried out moving and operation such as rotation on the three-dimensional, when sample arrives objective, strengthen the electric current on the miniature electric heating film 3, ice hockey is melted, promptly realize unloading this small items.
Description of drawings
Fig. 1 is the structural representation of the utility model ice tweezer;
The synoptic diagram that Fig. 2 fixes and operates small items for the utility model ice tweezer;
The synoptic diagram that Fig. 3 fixes and operates the small items in the liquid environment for the utility model ice tweezer.
Wherein: the fine probe 1 ice tweezer 2 miniature electric heating films 3 of popping one's head in
Temperature sensor 4 semiconductor coolers 5 semiconductor chilling plates 51
Semiconductor heat-dissipating sheet 52 liquid environments 86 sample stage 87
Power supply 53 leads 54 ice hockeys 6
Computer 83 microscopes 84 containers 85
Embodiment
Further describe the utility model below with reference to the drawings and specific embodiments:
See also Fig. 1 and Fig. 2, Fig. 1 is the structural representation of the utility model ice tweezer, the synoptic diagram that Fig. 2 fixes and operates small items for the utility model ice tweezer.By Fig. 1 and Fig. 2 as can be known, the ice tweezer that is used to handle small items that the utility model provides is characterized in that, this ice tweezer comprises:
Semiconductor refrigerator 5,
One fine probe 1, an end of this fine probe 1 contacts with semiconductor cooler 5 cold junctions, the fixedly connected at least one ice tweezer probe 2 of the other end, and
One miniature electrical-heating film 3, this miniature electrical-heating film 3 are coated on the part or all of outside surface of fine probe 1 and ice tweezer probe 2;
Described semiconductor cooler 5 is made up of semiconductor cooling piece 51, one heat radiator 52 and a power supply 53, and power supply 53 is electrically connected with semiconductor chilling plate 51 and heat radiator 52 by lead 54; After semiconductor chilling plate 51 and semiconductor heat-dissipating sheet 52 encapsulate, be connected with power supply 53 by lead 54, and realize the refrigeration and the rewarming of this semiconductor chilling plate 51 by a switch, described semiconductor chilling plate is a cube, and its length is of a size of 1-50mm * 1-50mm * 0.1-50mm;
Described fine probe 1 is a right cylinder, and its diameter is 1mm-5mm, and length is 5mm-50mm, and described ice tweezer probe 2 is the cone into a single integrated structure with the fine probe of this cylinder type 1, and the cone tapering is 1-10 °;
The film that described miniature electrical-heating film 3 constitutes for the electrical heating wire that is coated with adiabatic membrane on a kind of its outside surface, described electrical heating wire is formed by nickel-chromium alloy filament winding system, and described adiabatic membrane is a poly tetrafluoroethylene; Described miniature electrical-heating film 3 thickness are 1nm-1mm;
Described fine probe 1 and ice tweezer probe 2 are made by high thermal conductivity material; Described high thermal conductivity material is a stainless steel, and copper is silver-colored or golden;
Described ice tweezer 2 outside surfaces of popping one's head in are provided with a temperature sensor 4; Described temperature sensor 4 is a thermocouple temperature sensor;
The ice tweezer that is used to handle small items that the utility model provides, also can further comprise an operating control device, this operating control device comprises that one is used for fixing described fine probe 1, and the microoperation platform 81 that it is moved as three dimensions, one is used to control the microoperation controller 82 of these microoperation platform 81 actions, and microoperation controller 82 is by a computer 83 control operations; Described operating control device also comprises a microscope 84 that is connected with computer 83.
The ice tweezer that is used to handle small items that the utility model provides because the tip of ice tweezer probe 2 is very little, thereby relatively is suitable for being used with microscope.Start semiconductor cooler 5 implement freezing after, the ice hockey 6 that can form by the tip at ice tweezer probe 2 is small items 7 fixedly, and is operated (mode as shown in Figure 2 can be realized this goal) on three-dimensional.At pop one's head in the local of 2 outside surfaces or all cover miniature electrical-heating film 3 on the outside surfaces of fine probe 1 and ice tweezer, the inside of this electrical-heating film is equipped with electrical heating wire 31, by the control heating current, not only can control the size that forms ice hockey 5, and can unload fixing small object 6, can realize thus in the liquid environment or on the surface small items with liquid fix, move, complex operations such as rotation, collision, unloading; The surface of ice tweezer probe 2 is provided with temperature sensor 4, is specially thermocouple temperature sensor, can monitor and feed back the temperature of ice tweezer probe;
See also Fig. 3, the synoptic diagram that it is fixed and operate the small items in the liquid environment for the utility model ice tweezer.Operating control device in this ice tweezer comprises microoperation platform 81, microoperation controller 82, and computer 83, microscope 84, container 85, liquid environment 86, sample stage 87, and by connecting in proper order among Fig. 3.Wherein, microoperation platform 81 to control the motion of this fine probe 1 at three-dimensional, reaches micrurgic purpose by the fixing fine probe 1 of the clamping device on it; Microoperation controller 82 and computer 83 are the automatic control system of a whole set of instrument, by special software micromanipulation are controlled; Microscope 84 is instruments of observing microworld, and it is connected with computer 83 by data collecting card; Fine probe is installed on the microoperation platform 81, under the control of microoperation controller 82 and computer 83, is implemented in the small items of handling on the three-dimensional in the liquid environment 86 7.Because it is very little that fine probe 1 and ice tweezer probe 2 can be worked into, thereby relatively be suitable for observing for 84 times at microscope.
Use the ice tweezer in the present embodiment, be opposite to small items in the liquid environment and fix with manipulation process and be achieved in that
A) fine probe 1 and the ice tweezer probe 2 to the ice tweezer cleans, and after the sterilization, should ice tweezer and be fixed on the microoperation platform 81, starts microoperation controller 82, computer 83 and microscope 84;
B) under the observation of microscope 84, fine probe to be stretched in the liquid environment 86, the tip 21 of ice tweezer probe 2 comes close to or in contact with target small items 7.Be communicated with the circuit of semiconductor cooler 5, set the size of current on the miniature electrical-heating film 3 simultaneously; After several seconds, ice hockey 6 promptly forms on the surface of small items 7 towards ice tweezer probe 2, and then small object 7 is fixed;
C), can carry out moving and operation such as rotation on the three-dimensional to the small items 7 of the tip 21 that is fixed on ice tweezer probe 2 in conjunction with microscope 84 and microoperation instrument 82.When sample arrives objective, by adjusting the size of current on the miniature electrical-heating film 3, ice hockey 6 is melted, promptly realize unloading to small object 10.
In the practical application, a plurality of ice tweezer probes 2 can use simultaneously, to fix or to handle small items such as observation of minute biological samples or biological cell etc. better.Ice tweezer probe 2 also can be used with other micromanipulation probes, can realize the built-in function to observation of minute biological samples or biological cell etc., as extracting and the adding inhereditary material research contents that cell membrane punching etc. are complicated.
Under the on-liquid situation, the utility model can realize equally to the fixing of small object and operation, and this is having important application aspect carrying of the small items of many micro-/ nano manufacture fields and the manipulation.Such as by the tiny sampler surface is covered one deck fluid film in advance, ice tweezer probe 2 can freeze and finishes extracting or unloading to sample this fluid film.In addition, by ice tweezer of the present utility model, also can grasp or operation such as unloading objects such as the fine particles in the humid air.
When the tip of ice tweezer probe 2 near or when touching the small items that has moisture content or place liquid environment, can between the two, form an ice hockey 6 rapidly (because the adhesion of the two is very big, this small ice hockey can drive the very big object of quality), this is because in fact small items 7 has served as an icing nucleation zone towards the surface of ice tweezer probe tip, thereby freeze and very easily to occur in the surface rather than liquid on every side of small items 7, so the ice tweezer is handled small items and is often seemed accurately and fast.And the size of ice hockey 6 and temperature thereby are quite safe to the operation of the small items in the liquid 7 because the existence of miniature electrical-heating film 3 can control fully.
The utlity model has lot of advantages: at first, adopt the semiconductor Refrigeration Technique can accelerate to form the speed of ice hockey 6 in the tip of ice tweezer probe 2, easy to use, can realize multiple regulation and control scheme by the heating current size that changes miniature electrical-heating film 3; On the other hand, because that the ice tweezer is popped one's head in is 2 minimum, the ice hockey 6 of generation can accurately be fixed and handle small items, and can not damage small items such as biological sample; This ice tweezer can use on existing micromanipulation system, need not to introduce other devices, and compact conformation is more favourable for experimental study.Just because of these composite factors, make that the manufacturing cost price of the utility model ice tweezer is lower, the technology of comparing in the past such as light tweezer have certain advantage in some aspects.
Claims (12)
1, a kind of ice tweezer that is used to handle small items is characterized in that, this ice tweezer comprises:
Semiconductor refrigerator (5),
One fine probe (1), an end of this fine probe (1) contacts with semiconductor cooler (5) cold junction, the fixedly connected at least one ice tweezer probe (2) of the other end, and
One miniature electrical-heating film (3), this miniature electrical-heating film (3) are coated on the part or all of outside surface of fine probe (1) and ice tweezer probe (2).
2, the ice tweezer that is used to handle small items as claimed in claim 1, it is characterized in that, described semiconductor cooler (5) is by semiconductor cooling piece (51), and a heat radiator (52) and a power supply (53) are formed, and power supply (53) is electrically connected with semiconductor chilling plate (51) by lead (54).
3, the ice tweezer that is used to handle small items as claimed in claim 1, it is characterized in that, described fine probe (1) is a cylinder type, its diameter is 1mm-5mm, length is 5mm-50mm, described ice tweezer probe (2) is and the into a single integrated structure cone of the fine probe of this cylinder type (1), 1-10 ° of its cone tapering.
4, the ice tweezer that is used to handle small items as claimed in claim 1, it is characterized in that, the film that described miniature electrical-heating film (3) constitutes for the electrical heating wire that is coated with adiabatic membrane on a kind of its outside surface, described electrical heating wire is formed by nickel-chromium alloy filament winding system, and described adiabatic membrane is a poly tetrafluoroethylene.
As claim 1 or the 3 described ice tweezers that are used to handle small items, it is characterized in that 5, described fine probe (1) and ice tweezer probe (2) are made by high thermal conductivity material.
6, the ice tweezer that is used to handle small items as claimed in claim 5 is characterized in that, described high thermal conductivity material is a stainless steel, and copper is silver-colored or golden.
7, as claim 1 or the 4 described ice tweezers that are used to handle small items, it is characterized in that described miniature electrical-heating film (3) thickness is 1nm-1mm.
8, the ice tweezer that is used to handle small items as claimed in claim 1 or 2 is characterized in that, described semiconductor chilling plate is a cube, and its length is of a size of 1-50mm * 1-50mm * 0.1-50mm.
9, the ice tweezer that is used to handle small items as claimed in claim 1 is characterized in that, described ice tweezer probe (2) outside surface is provided with a temperature sensor (4).
10, the ice tweezer that is used to handle small items as claimed in claim 9 is characterized in that, described temperature sensor (4) is a thermocouple temperature sensor.
11, the ice tweezer that is used to handle small items as claimed in claim 1, it is characterized in that, also further comprise an operating control device, this operating control device comprises that one is used for fixing described fine probe (1), and the microoperation platform (81) that it is moved as three dimensions, one is used to control the microoperation controller (82) of this microoperation platform (81) action, and microoperation controller (82) is by a computer (83) control operation.
12, the ice tweezer that is used to handle small items as claimed in claim 11 is characterized in that, described operating control device also comprises a microscope (84) that is connected with computer (83).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02257290U CN2574187Y (en) | 2002-10-10 | 2002-10-10 | Ice forceps for operating microbody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02257290U CN2574187Y (en) | 2002-10-10 | 2002-10-10 | Ice forceps for operating microbody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2574187Y true CN2574187Y (en) | 2003-09-17 |
Family
ID=33725289
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN02257290U Expired - Lifetime CN2574187Y (en) | 2002-10-10 | 2002-10-10 | Ice forceps for operating microbody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2574187Y (en) |
-
2002
- 2002-10-10 CN CN02257290U patent/CN2574187Y/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050615 |
|
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050615 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |