Connect public, paid and private patent data with Google Patents Public Datasets

Integrated microfluid and microchip of microarray probe

Info

Publication number
CN2559986Y
CN2559986Y CN 02247727 CN02247727U CN2559986Y CN 2559986 Y CN2559986 Y CN 2559986Y CN 02247727 CN02247727 CN 02247727 CN 02247727 U CN02247727 U CN 02247727U CN 2559986 Y CN2559986 Y CN 2559986Y
Authority
CN
Grant status
Grant
Patent type
Prior art keywords
cell
detection
microfluid
model
utility
Prior art date
Application number
CN 02247727
Other languages
Chinese (zh)
Inventor
温龙平
唐飞
连崑
Original Assignee
上海博昇微晶科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Grant date

Links

Abstract

The utility model relates to the microchip of an integration microfluid and a micro array probe, which is characterized in that the microchip comprises a sample cell, a reagent cell, a detection cell, and a microfluid pipe, and each sample cell, reagent cell, and detection cell are connected through each microfluid pipe. The micro array probe is arranged at the bottom of the detection cell. The microchip also comprises a waste liquid cell which is connected with the detection cell through another microfluid pipe. The utility model has excellent parallel detection ability and can realize large amount sample detection at one time, and the utility model also has the function of fast pre-processing and separating sample, thus greatly enhancing the ability of the chip. The utility model is a chip with high integration capacity and strong parallel detection ability, which can reduce the complexity of detection cost and operation. The utility model is applicable to gene, biochemical, and pharmaceutical industries.

Description

集成微流体和微阵列探针的微芯片 Integrated microarray probes and microfluidic microchip

本实用新型属于生物芯片领域,是一种集成微流体和微阵列探针的微芯片。 The present invention belongs to the field of bio-chip, it is an integrated microarray probes and microfluidic microchip.

背景技术 Background technique

当前的生物芯片(有人称微芯片)按作用原理分,包括两类。 Current biochips (Some have called microchip) according to the principle of action points, including two categories.

一类是微流体芯片。 One is a microfluidic chip. 微流体芯片通过控制液体在芯片上的流动达到对各种生物组分的检测,也称为LAB-ON-CHIP,即在一个芯片上完成实验室的各项功能。 Microfluidic chip by controlling the flow of liquid on the chip reaches the detection of various biological components, also called functions LAB-ON-CHIP, i.e., completed in the laboratory on a chip. 微流体芯片上液体的控制和各种处理步骤是通过在芯片中制作微过滤器、微阀、微泵、微管道、微反应腔等各种精细微结构来实现的。 The microfluidic chip and various processing steps to control the liquid produced by the micro-filter in the chip, a variety of fine microstructures microvalves, micropumps, micro-channel, micro-reaction chamber and the like to achieve. 本文中所述的微流体芯片就是指上述定义的芯片。 Herein refers to microfluidics chip as defined above. 微流体芯片的特点是微型化和集成性优异。 Features microfluidic chip miniaturization and integration is excellent. 所谓的微型化是指生化检测可以在大大缩小的空间及体积中进行,从而大幅度减小试剂和样品的用量,降低检测成本;所谓的集成性是指在单一芯片上实现检测所需的所有或大部分步骤(如:样品的处理、分离、反应和检测)。 Refers to the so-called miniaturized biochemical assays can be greatly reduced in the space and volume, thereby greatly reducing the amount of reagents and samples, reducing testing costs; the so-called integration means to achieve the desired detection on a single chip all or most of the steps (eg: sample handling, separation, and detection reaction). 集成性高可以使检测自动化、标准化和统一化,使检测过程容易操作。 High integration can automate the detection, standardization and unification, the detection process is easy to operate. 微流体芯片在这两个方面具有优异的性能。 The microfluidic chip has excellent performance in these two areas. 但是,微流体芯片的一个缺点就是平行性较差,也就是说不能方便地实现在单个芯片上实现较多数量的样品检测。 However, a disadvantage is parallel microfluidic chip is poor, that is not easy to achieve a larger number of sample detection implemented on a single chip. 微流体芯片的这个缺点限制了它的检测能力和检测通量。 The microfluidic chip disadvantage limits its testing capability and throughput.

另一类芯片是微阵列芯片。 Another type of chip is a microarray chip. 微阵列芯片是将二维的芯片表面或者三维的颗粒表面分成许多相同的区域,每个区域产生一种探针用于特异性检测一种靶分子。 Microarray chip surface is two-dimensional or three-dimensional surface of the particles into many of the same regions, each generation of specific probes for detecting one kind of target molecule. 这里所谓的微阵列是指相同的探针或者不同的探针按照预先设计的图形在芯片上进行分布。 Here, the term microarray probes refer to the same or a different distribution of probes on the chip according to a predetermined pattern design. 如图3所示,这种阵列可以是相同或者不同探针6的点陈式阵列,也可以是相同或者不同探针6A-6F成块状(图4)、条形(图5)或者探针6G、6H、6J成任意其它形状的分布(图6),本文中所述的微阵列芯片以及微阵列就是指上述定义的概念。 3, such an array may be the same or different locations for the probe arrays 6 may be the same or different probes to block 6A-6F (FIG. 4), the strip (FIG. 5) or Probe needle 6G, 6H, 6J distribution into any other shape (FIG. 6), described herein as well as the microarray chip is a microarray as defined above refers to the concept.

微阵列芯片的探针的制作有点样和原地合成等方法。 The method of making a probe microarray chip bit samples and in situ synthesis. 具体的过程就是将生物分子(寡聚核苷酸、cDNA、基因组DNA、多肽、抗原、抗体等)固定于硅片、玻璃片等固相介质上形成生物分子点阵。 Specific process is biomolecules (oligonucleotides, cDNA, genomic DNA, polypeptides, antigens, antibodies, etc.) is fixed on a silicon wafer, glass and other biological molecules forming the solid phase medium dot. 在待分析样品中的生物分子与生物芯片的探针分子发生杂交或相互作用后,利用扫描仪对杂交信号进行检测和分析。 After the probe biomolecule of the bio-chip sample to be analyzed or hybridization interaction occurs, a scanner on the hybridization signal is detected and analyzed. 目前应用最广泛的微阵列芯片是基因芯片,它采用OLIGO或DNA片断为探针,检测DNA或mRNA。 Currently the most widely used is microarray gene chip, which uses OLIGO or DNA fragment as a probe to detect DNA or mRNA. 微阵列芯片具有微型化的优点。 Microarrays have the advantage of miniaturization. 它的另一个很重要的优点就是平行性优异,也就是说它可以在单一的芯片上同时实现很多个靶分子或者样品的检测,因而可以实现高通量检测。 It is another important advantage is excellent in parallel, that is, it enables simultaneous detection of a target molecule or a number of samples on a single chip, it is possible to achieve high-throughput assay. 例如Affymetrix公司研制的基因表达谱芯片的点阵数可以达到400,000个点。 For example, the number of dot-matrix developed by the company Affymetrix gene expression profile chip can reach 400,000 points. 但是微阵列芯片很难将样品的预处理等分析前的工作步骤集成到一个芯片上来完成,而只能依靠人工或者专门的仪器来实现这些步骤,也就是说它的集成性不好,因而浪费了大量的人力和物力,而且系统自动化程度不高,不容易标准化。 However, it is difficult to microarray chip pretreatment of the sample before analysis, and the like integrated into one chip up step is completed, and only rely on manual or specialized equipment to implement these steps, that is to say its poor integration, thereby wasting a lot of manpower and material resources, and the system is not high degree of automation, standardization is not easy. 如果将微阵列芯片和微流体芯片在同一芯片上实现,就可以兼顾两种芯片的特点,实现优势互补,在保证微型化的基础上,同时达到巨大的平行性和优异的集成性。 If the microarray chip and microfluidics chip on the same chip, can take into account the characteristics of two chips, complementary advantages, ensuring miniaturization basis, while achieving great parallel resistance and excellent integration. 可是目前未见这种集成芯片的报道。 But at present no such integrated chips reported.

本实用新型的内容针对目前生物芯片开发的现状和上述分析,本实用新型的目的就是将微流体芯片和微阵列芯片的功能集成在一起,实现一种既具有微流体芯片的高度集成性,又具有微阵列芯片强大的并行检测能力的特点的新型芯片和方法。 The present invention is directed to the development of the current status of the biochip and the above analysis, object of the present invention is to integrate the microfluidic chip and microarray chip function together to achieve both a high degree of integration with the microfluidic chip, and novel features of the method and chip having microarray powerful parallel detection capability.

本实用新型的目的是通过以下技术方案实现的,本实用新型一种集成微流体和微阵列探针的微芯片,其特征是:在所述的微芯片上具有试剂池和微流体管道,各试剂池通过各微流体管道相连。 The object of the present invention is achieved by the following technical solutions, the microchip of the present invention and an integrated microfluidic microarray probes, characterized in that: a reagent reservoir and the microfluidic conduit on the microchip, each reagent cells are connected through the microfluidic conduit. 为了表达方便,将存放试剂、样品或混合液的腔体统称为试剂池。 For convenience of expression, the reagent store, a sample or mixture is referred to collectively as the reagent cell chamber. 在所述的各试剂池中,一个或几个试剂池的底部上具有微阵列探针。 The reagents in the pool, or on the bottom of a reagent cell having several microarray probes. 上述带微阵列探针的试剂池又称为检测池。 The tape microarray probe reagent cells, also known as detector cell. 所述试剂池还可包括样品池以及废液池,所述废液池可经另一微流体管道与检测池相连。 The reagent may further comprise a sample cell and cell waste reservoir, a waste reservoir may be connected via another conduit and the microfluidic flow cell. 本实用新型当用于检测应用时,其检测步骤举例如下:样品首先被注入样品池中,然后由样品池流入检测池。 When the present invention is applied for detecting, detecting steps include the following: First, a sample is injected into the sample cell, the detection cell and then flows into the sample cell. 在检测池中,样品与检测池底部的微阵列探针发生反应,反应完成后,废液流入废液池;然后,各种试剂分别从各自的试剂池中顺序流入检测池,对反应物进行清洗或反应。 The reaction occurs in the detection cell, the detection cell bottom sample with microarray probes, after the completion of the reaction, the waste liquid into the waste tank; then, the various reagents are sequentially flows from the respective detection cells reagent cell, the reaction was cleaning or reaction. 每种试剂在充分清洗或反应后被排入废液池。 Each reagent is discharged into a waste reservoir after being sufficiently washed or reaction. 之后,用光学或其它检测装置对检测池中的反应物进行检测。 Thereafter, optical or other detection means detects the reaction cell was detected. 整个过程只需要注入样品(可能也包括试剂),剩下的全部操作都在芯片上自动完成。 The whole process takes sample injection (also may include reagent), the remaining operations are all done automatically on the chip. 目前的微阵列芯片的样品需要先进行外部预处理,然后才能用微阵列芯片进行检测,而且各种预处理过程全部是靠手工或者其它专门仪器来完成。 The current sample microarray chip external need pretreatment before they can be detected by microarray, and various pre-processes all by hand, or other specialized equipment to complete. 在本实用新型芯片中,则不需要这些外部预处理步骤,原来的外部预处理步骤现在完全或者大部分可以在本实用新型芯片上进行。 In the present invention, the chip, the external preprocessing step is not required, the original exterior now fully or mostly pretreatment step may be performed on the chip of the present invention. 在现有的微流体芯片中,检测池中一般只有一种探针或反应物,也只能检测一种样品或者一种成分,而在本实用新型芯片中,由于在检测池的底部制作了微探针的阵列,所以可以同时检测多种样品或者样品的多种成分。 In conventional microfluidic chip, generally only one probe in the detector cell or reactant, can only be detected in a sample or one component, whereas in the present invention, the chip, since the bottom of the detector cell produced an array of micro probes, can be detected more of a variety of components of the sample or sample simultaneously. 因而本实用新型芯片的功能更为全面。 Thus the invention of the chip functions more comprehensive.

综上所述,本实用新型的优点主要有:1.与目前微流体芯片相比:由于检测腔中制作了微阵列探针,本实用新型芯片具有更优异的并行检测能力,比现有的微流体芯片检测范围更为强大,一次可以实现大数目样品的检测;2.与现有的微阵列芯片相比:由于集成了具有快速样品前处理和分离功能的微流体芯片的能力,本实用新型芯片不需要额外的、复杂的、耗费人力和物力的样品前处理和分离工作,大大提高了芯片的功能、降低了检测的成本和操作的复杂性,因而系统的自动化程度更高,更易于标准化。 In summary, the advantages of the present invention are: 1 compared with the current microfluidic chips: Since the detection chamber fabricated in the microarray probes, the present invention has more excellent chip parallel detection capability than conventional microfluidic chip detection range of more powerful, one can achieve the detection of a large number of samples; 2 compared to the conventional microarray chip: Since the ability to pre-fast integrated sample handling and separation function microfluidic chip, the present invention no additional new chip, complex, separation and work-consuming process before a sample of human and material resources, greatly improving the functions of the chip, reducing the cost and complexity of the operation is detected, and thus a higher degree of automation, easier standardization.

附图说明 BRIEF DESCRIPTION

图1为本实用新型微芯片的示意图,图2为本实用新型的使用情况图,图3-6为各种形状的探针微阵列示意图。 Figure 1 is a schematic view of a microchip invention, FIG. 2 of the present invention use, and FIG. 3-6 is a schematic view of various shapes of the probes of the microarray.

图中代号说明I芯片 II转台1检测池 2废液池 3微管道4试剂池 5样品池 6、6A-6J微阵列探针7、8微管道实施例利用LIGA工艺和微阵列芯片制作工艺相结合的方法,在PMMA上制作微流体和微阵列集成的芯片。 FIG Code Description I II turret detecting chip 2 cell micro channel waste reservoir 3 4 5 reagent cell sample cell 6,6A-6J microarray probes 7,8 LIGA process using a micro-pipe embodiment and phase microarray chip fabrication process embodiment the method of binding, and making a microfluidic chip integrated microarray on PMMA. 该芯片的检测池中制作有微阵列探针,可以同时进行多个样品的检测,芯片的制作采用我们申请的另一项独立专利制作;芯片中液体的驱动原理采用离心力驱动的方法。 Detection of the production cell chip microarray probes, multiple samples can be detected simultaneously, making use of the chip A separate patent application of our production; chip driving principle of the liquid-driven centrifugal method. 关于这种离心力的微流体控制系统,我们申请了另一项独立的发明专利技术;芯片的检测系统也申请了另一项独立的发明专利;芯片I的结构如图1所示。 About micro fluid control system of this centrifugal force, we filed another patent technology independent; chip detection system is also applied for another separate patent; I chip structure shown in Figure 1. 如图1,在本实用新型芯片I上具有样品池5、试剂池4、检测池1、废液池2和各微管道3、7、8,各试剂池4通过各微管道3与样品池5相连,样品池5经微管道7与检测池1相连,在检测池1的底部具有微阵列探针6,可同时进行多个样品的检测,在检测池1的一端经微管道8与废液池2相通连。 1, having a sample cell 5, 4 agent pool, the detection cell 1, cell 2, and each waste 3,7,8 microchannel, each reagent cell and the sample cell 43 through the micro-channel chip in the present invention I 5 is connected to the sample cell 5 via conduit 7 is connected to the micro-detection cell 1, a microarray having a detection probe 6 in the bottom of cell 1, which can detect a plurality of samples of the waste by the micro-conduit 8 at one end of the detection cell 1 2 for communication of the liquid pool. 如图2,可将本芯片I放在转台II上,通过转台II绕其中心O的旋转,和调节该芯片I绕自己的轴心O′转动的角度θ,使离心力F大于管道的毛细管阻力,将液体从一个池送到另一个池中。 2, this chip can be placed on the turntable I II, about its center of rotation O by the turntable II, I, and adjusting the chip about its own axis O 'of the rotated angle θ, so that the centrifugal force F is greater than the resistance of the capillary conduit the liquid from one tank to another tank.

芯片I的工作过程如下:各种试剂被预先存贮在各自的试剂池4中。 I chip working process is as follows: the various reagents are previously stored in the respective reagent reservoir 4.

检测时,首先将待测样品滴入样品池5;在离心力F的作用下,样品经微管道7流入检测池1与检测池底部的微阵列探针6发生反应;反应充分后,通过离心力的控制,样品经微管道8流入废液池2;利用离心力,微流体控制系统通过使不同的试剂从各自的试剂池4顺序进入检测池1对探针进行清洗或反应,清洗或反应充分后,废液经微管道8流入废液池2;在上述预处理过程和反应完成后,利用检测系统对检测池中的微阵列探针6进行检测,定性或定量确定样品的成分。 When detected, the sample to be tested is first dropped into the sample cell 5; F by the centrifugal force, the sample flows through the micro-pipe 7 and the detection cell 1 cell bottom microarray probes react 6; After sufficient reaction, by centrifugal force control samples were micro-pipe 8 into the waste reservoir 2; use of centrifugal force, after the microfluidic control system by sufficiently different reagents from the respective reagent into the detection cell 4 cell 1 sequence probe or washing the reaction, the reaction or washing, waste via conduit 8 into the waste micro cell 2; after the above pretreatment and the reaction process, using the detection system of the microarray probe 6 detects pool, qualitatively or quantitatively determine the composition of the sample.

Claims (2)

1.一种集成微流体和微阵列探针的微芯片,其特征是:在所述的微芯片上具有试剂池和微流体管道,各试剂池通过各微流体管道相连;在所述各试剂池中,一个或几个试剂池的底部上具有阵列探针。 An integrated microchip and microfluidic microarray probes, characterized in that: a reagent reservoir and the microfluidic conduit on the microchip, each reagent reservoir through the micro-fluid conduit is connected; in each of the reagent having an array of probes on the bottom of the pool, the pool of one or several reagents.
2.根据权利要求1所述的集成微流体和微阵列探针的微芯片,其特征是:所述试剂池还包括样品池、废液池。 The integrated microfluidic microchip of claim 1 and claim microarray probes, wherein: said sample cell further comprises a reagent reservoir, the waste reservoir.
CN 02247727 2002-08-23 2002-08-23 Integrated microfluid and microchip of microarray probe CN2559986Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 02247727 CN2559986Y (en) 2002-08-23 2002-08-23 Integrated microfluid and microchip of microarray probe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 02247727 CN2559986Y (en) 2002-08-23 2002-08-23 Integrated microfluid and microchip of microarray probe

Publications (1)

Publication Number Publication Date
CN2559986Y true CN2559986Y (en) 2003-07-09

Family

ID=33718542

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 02247727 CN2559986Y (en) 2002-08-23 2002-08-23 Integrated microfluid and microchip of microarray probe

Country Status (1)

Country Link
CN (1) CN2559986Y (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7291497B2 (en) 2003-09-11 2007-11-06 Theranos, Inc. Medical device for analyte monitoring and drug delivery
US7888125B2 (en) 2005-05-09 2011-02-15 Theranos, Inc. Calibration of fluidic devices
US8007999B2 (en) 2006-05-10 2011-08-30 Theranos, Inc. Real-time detection of influenza virus
CN1786710B (en) 2004-12-06 2011-12-14 财团法人工业技术研究院 A microfluidic chip sample analyzer and method
US8158430B1 (en) 2007-08-06 2012-04-17 Theranos, Inc. Systems and methods of fluidic sample processing
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US8778665B2 (en) 2006-11-14 2014-07-15 Theranos, Inc. Detection and quantification of analytes in bodily fluids
US8862448B2 (en) 2009-10-19 2014-10-14 Theranos, Inc. Integrated health data capture and analysis system
CN104374903A (en) * 2014-11-08 2015-02-25 东莞博识生物科技有限公司 In vitro diagnosis test card
CN105296348A (en) * 2015-11-20 2016-02-03 融智生物科技(青岛)有限公司 Genotyping detection-based microfluidic chip, detection system and device

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7291497B2 (en) 2003-09-11 2007-11-06 Theranos, Inc. Medical device for analyte monitoring and drug delivery
US8101402B2 (en) 2003-09-11 2012-01-24 Theranos, Inc. Medical device for analyte monitoring and drug delivery
CN1786710B (en) 2004-12-06 2011-12-14 财团法人工业技术研究院 A microfluidic chip sample analyzer and method
US8679407B2 (en) 2005-05-09 2014-03-25 Theranos, Inc. Systems and methods for improving medical treatments
US9182388B2 (en) 2005-05-09 2015-11-10 Theranos, Inc. Calibration of fluidic devices
US9075046B2 (en) 2005-05-09 2015-07-07 Theranos, Inc. Fluidic medical devices and uses thereof
US8283155B2 (en) 2005-05-09 2012-10-09 Theranos, Inc. Point-of-care fluidic systems and uses thereof
US7888125B2 (en) 2005-05-09 2011-02-15 Theranos, Inc. Calibration of fluidic devices
US9772291B2 (en) 2005-05-09 2017-09-26 Theranos, Inc. Fluidic medical devices and uses thereof
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US9176126B2 (en) 2006-03-24 2015-11-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US8669047B2 (en) 2006-05-10 2014-03-11 Theranos, Inc. Real-time detection of influenza virus
US8007999B2 (en) 2006-05-10 2011-08-30 Theranos, Inc. Real-time detection of influenza virus
US8778665B2 (en) 2006-11-14 2014-07-15 Theranos, Inc. Detection and quantification of analytes in bodily fluids
US8883518B2 (en) 2007-08-06 2014-11-11 Theranos, Inc. Systems and methods of fluidic sample processing
US9575058B2 (en) 2007-08-06 2017-02-21 Theranos, Inc. Systems and methods of fluidic sample processing
US8158430B1 (en) 2007-08-06 2012-04-17 Theranos, Inc. Systems and methods of fluidic sample processing
US8862448B2 (en) 2009-10-19 2014-10-14 Theranos, Inc. Integrated health data capture and analysis system
US9460263B2 (en) 2009-10-19 2016-10-04 Theranos, Inc. Integrated health data capture and analysis system
CN104374903A (en) * 2014-11-08 2015-02-25 东莞博识生物科技有限公司 In vitro diagnosis test card
CN105296348A (en) * 2015-11-20 2016-02-03 融智生物科技(青岛)有限公司 Genotyping detection-based microfluidic chip, detection system and device

Similar Documents

Publication Publication Date Title
Chován et al. Microfabricated devices in biotechnology and biochemical processing
Miller et al. A digital microfluidic approach to homogeneous enzyme assays
Fair et al. Electrowetting-based on-chip sample processing for integrated microfluidics
Boone et al. Peer reviewed: plastic advances microfluidic devices
Ducrée et al. The centrifugal microfluidic Bio-Disk platform
Jakeway et al. Miniaturized total analysis systems for biological analysis
Zoval et al. Centrifuge-based fluidic platforms
US7186383B2 (en) Miniaturized fluid delivery and analysis system
US6720157B2 (en) Chips having elevated sample surfaces
US6284113B1 (en) Apparatus and method for transferring liquids
US20040109793A1 (en) Three-dimensional microfluidics incorporating passive fluid control structures
US20040086870A1 (en) Microfluidic system for analyzing nucleic acids
US6960467B2 (en) Biochannel assay for hybridization with biomaterial
US6551836B1 (en) Microfluidic devices, systems and methods for performing integrated reactions and separations
US6444461B1 (en) Microfluidic devices and methods for separation
Gervais et al. Microfluidic chips for point‐of‐care immunodiagnostics
US20060246490A1 (en) Miniaturized genetic analysis systems and methods
US6556923B2 (en) Software for high throughput microfluidic systems
US20020092767A1 (en) Multiple array microfluidic device units
US6677131B2 (en) Well frame including connectors for biological fluids
US20030044322A1 (en) Retaining microfluidic microcavity and other microfluidic structures
Hong et al. Micro-and nanofluidic systems for high-throughput biological screening
US6875619B2 (en) Microfluidic devices comprising biochannels
US6406893B1 (en) Microfluidic methods for non-thermal nucleic acid manipulations
US20070042427A1 (en) Microfluidic laminar flow detection strip

Legal Events

Date Code Title Description
C14 Granted
C19 Lapse of patent right due to non-payment of the annual fee