CN2556643Y - Electrochemical biological sensor - Google Patents
Electrochemical biological sensor Download PDFInfo
- Publication number
- CN2556643Y CN2556643Y CN 01272180 CN01272180U CN2556643Y CN 2556643 Y CN2556643 Y CN 2556643Y CN 01272180 CN01272180 CN 01272180 CN 01272180 U CN01272180 U CN 01272180U CN 2556643 Y CN2556643 Y CN 2556643Y
- Authority
- CN
- China
- Prior art keywords
- utility
- model
- detect
- biosensor
- polydiacetylene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model relates to an electrochemistry biosensor, pertaining to the microbial detection technical field and comprises a silicon chip, a gold film, a support layer, a detecting layer, a receptor, an electrolytic cell, a circulating volt-ampere scanner and a computer. The utility model adopts the self-assembly technique and realizes the embellishment of a working electrode surface by using thiol, polydiacetylene and a polydiacetylene detecting layer with the receptor, furthermore, the utility model uses cyclic voltammetry to detect the response value of the electrode and the current after germiculture and prepares the electrochemistry biosensor to detect bacteria. The theory of the utility model is that the structure of the polydiacetylene in the detecting layer is influenced by the biomolecular recognition so that the transporting of the detect molecule and the electrode surface are hindered, thus producing extremely obvious current response. Compared with an original biosensor, the biosensor of the utility model has the advantages of simple preparation, strong specificity, etc. The utility model can detect the bacteria fast and has extremely important significance to the fields of medical diagnosis, food industry and environmental protection. The utility model is applicable to detecting the variety and consistency of microbes in surrounding environment, etc.
Description
Technical field: the utility model belongs to the microorganism detection technical field, relates to a kind of electrochemica biological sensor.
Background technology: well-known, microorganism is closely bound up with human life, and they have crucial influence to the mankind's life.For example, many bacteriums can cause a disease the people by discharging toxin; Also there are simultaneously many bacteriums to be utilized as human service by us.At present, the method for general bacterial detection is the physiology, morphological feature by measuring bacterium or identifies bacterium by the genomic constitution of measuring bacterium.The complicated operation of these methods, and need the cost long time, they can not satisfy current requirement to microorganism detection far away.In this case, many researchists explore new method hardy, so that bacterium is detected apace.People such as Charach in 1993 reported first on the science utilize poly-two alkyne films of having cell surface receptor with corresponding ligand molecular in conjunction with the time, the color of film can have been carried out direct colorimetric detection to influenza virus by the red this phenomenon of blue stain.This affinity metachromatism has caused people's extensive interest.Domestic have many units that this is studied, as Beijing sensitization people such as Jiang Long utilize the poly-two alkynes liposomes have acceptor that bacterium is detected.These researchs all concentrate on and utilize the optical property of poly-two alkynes that bacterium is detected, and the report that utilizes electrochemical method bacterial detection also seldom.
Detailed content: the purpose of this utility model is in order to solve the Bacteria Detection complicated operation, and need the long problem of spended time, to achieve these goals, the utility model is a unimolecular layer of utilizing numerator self-assembly technique deposition one deck microorganism sensitivity on golden film (2), contain silicon chip (1), gold film (2), supporting layer (3), detection layers (4), acceptor (5), electrolytic cell (6), cyclic voltammetry scan device (7), computing machine (8), it is characterized in that on silicon chip (1), being coated with golden film (2) with the evaporation coating method, supporting layer (3) preparation is on the surface of golden film (2), go up deposition one deck detection layers (4) at supporting layer (3), on the detection layers (4) acceptor (5), acceptor (5) places the electrolytic solution of electrolytic cell (6), three input ends of cyclic voltammetry scan device (7) respectively with golden film (2) and electrolytic cell (6) in contrast electrode with the utmost point is connected, the output terminal of cyclic voltammetry scan device (7) is connected with the input end of computing machine (8).
During the utility model work, golden film, supporting layer and detection layers are formed working electrode, and with the cyclic voltammetry scan device working electrode is carried out cyclic voltammetry scan, this moment, the peak current of writing task electrode was I
0Place working electrode certain density bacterial solution to cultivate then, again it carried out cyclic voltammetry scan, this moment record be working electrode with peak current after bacterium combines be I
XAnd calculate the response (I of electric current thus
X/ I
0) * 100%.The time that on behalf of working electrode, X combine with bacterium, X=1,2,3,4,5.......According to the method described above, measure working electrode and the electric current I of same concentrations microbe growth after 2,3,5 and 8 minutes respectively
XResponse, remake out the response curve of work electrode pair bacterium different time.Behind the Best Times that obtains to detect by this response curve, under this Best Times, measured the response curve of working electrode again to the variable concentrations bacterium.Simultaneously, we have tested again that working electrode is placed in the physiological saline and during the no acceptor in detection layers surface, working electrode is to the response curve of bacterium, and the result shows: this electrochemical sensor is specific to the detection of bacterium, and the sensitivity of its detection is very high.
Principle of the present utility model is the influence that the structure of detection layers is subjected to the identification of bacterium part and acceptor: promptly after the part of the acceptor on detection layers surface and bacterium surface is discerned, the structure of poly-two alkyne films becomes more orderly in the detection layers, the quantity of defective in poly-two alkyne film is reduced, then hindered in the electrolytic solution molecular detection to the transportation on golden film surface, thereby produced current-responsive very obvious.Simultaneously, the data of surveying can directly handle with computing machine, this also makes the speed of data processing accelerate greatly, helps the detection by quantitative of bacterium.
The utility model has the advantages that: adopt the utility model to solve the Bacteria Detection complicated operation, and need the long problem of spended time, but provide that a kind of easy preparation and test operation are simple and convenient, highly sensitive, the biology sensor of high specificity, short detection by quantitative bacterial concentration of response time, realized direct detection bacterium.The utility model can detect bacterium apace, all has crucial meaning at medical diagnosis, food industry and field of environment protection.The also available mercaptan of the utility model and poly-two alkynes and the poly-two alkynes bilayer detection layers that have other acceptor detect different bacteriums and virus.
Description of drawings:
Fig. 1 is the working electrode structural representation in the utility model
Fig. 2 is the utility model structural representation
Embodiment: the bilayer electrode includes as depicted in figs. 1 and 2: silicon chip (1), golden film (2), supporting layer (3), detection layers (4), acceptor (5), electrolytic cell (6), cyclic voltammetry scan device (7), computing machine (8),
Silicon chip (1) adopts monocrystalline silicon; Gold film (2) adopts 99% spun gold, and with the evaporation coating method spun gold is plated to upward formation golden film (2) of silicon chip (1); Supporting layer (3) adopts the Stearyl mercaptan molecule to be assembled in golden film (2) surface with self-assembling technique; Detection layers (4) adopt poly-two alkynes materials with bright Miao's technology (Langmuir-Blodgett) with its membrane to supporting layer (3); Acceptor (5) is connected with detection layers (4) by covalent bond, and acceptor (5) adopts can select mannose or sialic acid for use; Electrolytic cell (6) adopts the glass electrolytic cell.Cyclic voltammetry scan device (7) adopts CHI832.Computing machine (8) adopts common personal computer.
Claims (1)
1, a kind of electrochemica biological sensor, it is characterized in that: contain silicon chip (1), gold film (2), supporting layer (3), detection layers (4), acceptor (5), electrolytic cell (6), cyclic voltammetry scan device (7), computing machine (8), on silicon chip (1), be coated with golden film (2) with the evaporation coating method, supporting layer (3) preparation is on the surface of golden film (2), go up deposition one deck detection layers (4) at supporting layer (3), on the detection layers (4) acceptor (5), acceptor (5) places the electrolytic solution of electrolytic cell (6), three input ends of cyclic voltammetry scan device (7) respectively with golden film (2) and electrolytic cell (6) in contrast electrode with the utmost point is connected, the output terminal of cyclic voltammetry scan device (7) is connected with the input end of computing machine (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01272180 CN2556643Y (en) | 2001-12-12 | 2001-12-12 | Electrochemical biological sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01272180 CN2556643Y (en) | 2001-12-12 | 2001-12-12 | Electrochemical biological sensor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2556643Y true CN2556643Y (en) | 2003-06-18 |
Family
ID=33677392
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01272180 Expired - Fee Related CN2556643Y (en) | 2001-12-12 | 2001-12-12 | Electrochemical biological sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2556643Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102375009A (en) * | 2011-09-14 | 2012-03-14 | 东南大学 | Method for rapidly detecting bacteria by using electrochemical method |
| CN1981192B (en) * | 2004-05-14 | 2014-04-16 | 拜尔健康护理有限责任公司 | Voltammetric systems for assaying biological analytes |
| CN107151629A (en) * | 2017-04-25 | 2017-09-12 | 青岛天人环境股份有限公司 | The double rank desulfurizers of Intelligent biogas |
| CN108444930A (en) * | 2018-02-12 | 2018-08-24 | 中国科学院长春应用化学研究所 | A method of differentiating wine with polybutadiene alkynes probe array |
| CN108562631A (en) * | 2018-03-19 | 2018-09-21 | 青岛科技大学 | A kind of test method detecting anaerobic digestion based on electrochemical techniques |
-
2001
- 2001-12-12 CN CN 01272180 patent/CN2556643Y/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1981192B (en) * | 2004-05-14 | 2014-04-16 | 拜尔健康护理有限责任公司 | Voltammetric systems for assaying biological analytes |
| CN103901092B (en) * | 2004-05-14 | 2016-07-06 | 拜尔健康护理有限责任公司 | The voltammertry system of detection biological analyte |
| CN102375009A (en) * | 2011-09-14 | 2012-03-14 | 东南大学 | Method for rapidly detecting bacteria by using electrochemical method |
| CN102375009B (en) * | 2011-09-14 | 2013-09-18 | 东南大学 | Method for rapidly detecting bacteria by using electrochemical method |
| CN107151629A (en) * | 2017-04-25 | 2017-09-12 | 青岛天人环境股份有限公司 | The double rank desulfurizers of Intelligent biogas |
| CN107151629B (en) * | 2017-04-25 | 2019-06-28 | 青岛天人环境股份有限公司 | The double rank desulfurizers of Intelligent biogas |
| CN108444930A (en) * | 2018-02-12 | 2018-08-24 | 中国科学院长春应用化学研究所 | A method of differentiating wine with polybutadiene alkynes probe array |
| CN108444930B (en) * | 2018-02-12 | 2019-08-16 | 中国科学院长春应用化学研究所 | A method of identifying wine with polybutadiene alkynes probe array |
| CN108562631A (en) * | 2018-03-19 | 2018-09-21 | 青岛科技大学 | A kind of test method detecting anaerobic digestion based on electrochemical techniques |
| CN108562631B (en) * | 2018-03-19 | 2020-09-01 | 青岛科技大学 | Test method for detecting anaerobic digestion based on electrochemical technology |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ding et al. | Trends in cell-based electrochemical biosensors | |
| Qu et al. | A micro-potentiometric hemoglobin immunosensor based on electropolymerized polypyrrole–gold nanoparticles composite | |
| Chawla et al. | An amperometric hemoglobin A1c biosensor based on immobilization of fructosyl amino acid oxidase onto zinc oxide nanoparticles–polypyrrole film | |
| CN110736779A (en) | Preparation method of bifunctional hybrid film for self-calibration detection of tumor exosomes | |
| Wang et al. | Immunosensor based on electrodeposition of gold-nanoparticles and ionic liquid composite for detection of Salmonella pullorum | |
| CN107132260B (en) | A kind of electrochemical sensor based on nano material detection Ractopamine | |
| CN104730253B (en) | Detection strip based on click chemistry, detection method and application | |
| CN106525943B (en) | A kind of surface protein imprints construction method and its application of self energizing biological fuel cell sensor | |
| CN111693571A (en) | Method for detecting GPC3 based on optical addressing potential sensor | |
| CN110243891A (en) | A kind of label-free homogeneous electrochemical biosensor method detecting cancer cell | |
| Jiang et al. | A self-driven PET chip-based imprinted electrochemical sensor for the fast detection of Salmonella | |
| Ren et al. | Rapid detection of antibiotic resistance in Salmonella with screen printed carbon electrodes | |
| CN105954345A (en) | Method for electrochemically determining content of vitamins in fruit juice | |
| CN112432981A (en) | Single-cell electrochemical sensor based on functionalized nanoprobe and application thereof | |
| Li et al. | A microbial electrode based on the co-electrodeposition of carboxyl graphene and Au nanoparticles for BOD rapid detection | |
| CN108375616A (en) | A kind of liquid crystal biosensor of detection of alkaline phosphatase and its preparation method and application | |
| CN2556643Y (en) | Electrochemical biological sensor | |
| CN104493160A (en) | Gold nanorod biotic compound and preparation method and applications thereof | |
| CN110398527A (en) | Electric potential type saliva acid sensor based on molecularly imprinted polymer modification carbon cloth electrode | |
| CN110006971A (en) | A kind of preparation method and applications of the aptamer sensor of binary channels output detection food-borne pathogens | |
| TWI355418B (en) | Apparatus for cell number and cellular protein det | |
| CN1710418A (en) | Biological sensor for quickly detecting hemoglobin and detecting method thereof | |
| CN108956734A (en) | Sensor and its preparation method and application based on boric acid affinity interaction | |
| CN103823071B (en) | " magnetic bead glycolated hemoglobin ferrocene boric acid " complex and application thereof | |
| CN109406596A (en) | A kind of electrochemical sensor, preparation method and application detecting microRNA-21 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |