CN2544282Y - Mountable/dismountable microquantity dilute drug sensitivity reagent box - Google Patents
Mountable/dismountable microquantity dilute drug sensitivity reagent box Download PDFInfo
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- CN2544282Y CN2544282Y CN 02214306 CN02214306U CN2544282Y CN 2544282 Y CN2544282 Y CN 2544282Y CN 02214306 CN02214306 CN 02214306 CN 02214306 U CN02214306 U CN 02214306U CN 2544282 Y CN2544282 Y CN 2544282Y
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- kit
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model relates to a kit used for drug sensitive micro dilution in a clinical bacteria drug sensitive test, comprising a square bracket 2, in which an array or more than one array reaction plates 3 are arranged; wherein, the reaction plates are provided with an array of V-shaped reaction holes 4, and both ends of the reaction plates are respectively provided with a fixed bridge 5 through which the reaction plates are positioned in the square bracket; and a box cover 5 is positioned on the square bracket. The kit can not only be dismounted or assembled randomly according to different needs but also increase the amount of experimental medicine. Moreover, the kit can change concentration gradient of a part of the medicine according to clinical demands. The kit can also choose flexibly the amount and the type of the medicine according to the result of bacterial identification, which ensures a report of the drug sensitization to be more reasonable.
Description
Affiliated technical field
The utility model relates to and is used for the employed kit of Microdilution susceptibility in a kind of clinical bacteria drug sensitive experiment.
Background technology
Along with antibiotic widespread use, not even reasonably not abuse, drug-resistance of bacteria strengthens year by year, and the resistance bacterial classification occurs in a large number.The research of bacterial resistance mechanism is the emphasis and the difficult point of bacteriological study always.As everyone knows, fast, accurately, reasonably selecting active drug, is the important step that prevents that drug-fast bacteria from producing, and therefore, it is particularly important that external drug sensitive experiment seems.At present, the drug sensitive experiment method of clinical labororatory's use has disk diffusion method and micro-dilution method.Disk diffusion method is that the various antibacterials scraps of paper that will contain specific concentrations are attached on the MHA plate of various pure bacterium by clinical separation, hatch certain hour after, measure the diameter of antibacterial ring around the scraps of paper, report resistance, intermediary or sensitivity respectively.This method can freely be selected medicine, but it is quantitative to carry out medicine.It is quantitative that micro-dilution method carries out susceptibility, is bacterium liquid directly to be added contain in the micropore of dehydrated medium and variable concentrations kind antibacterials, and relatively each hole concentration change is determined the degree that bacterium is suppressed by antibacterials after cultivating.This method is a kind of method for quantitatively determining, can measure minimal inhibitory concentration, to " intermediary " result who occurs in the paper disk method susceptibility, when needing further to determine, can do for supplement MIC.At present, contained medication amount is few and immobilize in the kit of micro-dilution method susceptibility, makes tested medication amount limited, can not select medicine neatly according to measurement result.
Invention constitutes
The purpose of this utility model is to provide a kind of Microdilution susceptibility kit that can select medicine according to the Bacteria Identification result neatly.
The utility model is to realize like this.It comprises a square frame shape support, one row or the reaction plate more than the row are housed in this support, have row's V-arrangement reacting hole on this reaction plate, the two ends of this reaction plate respectively have a fixed bridge, through this fixed bridge reaction plate is installed in this square frame shape support, a lid also is housed on square frame shape support constitutes.
Because the reaction plate of this Microdilution susceptibility kit is by more than the row, every row is made up of a plurality of V-arrangement reacting holes, not only arbitrarily dismounting combination as required can also increase the quantity of experiment medicine, and can change part drug concentrations gradient according to clinical needs.This kit can also be selected the value volume and range of product of medicine neatly according to the Bacteria Identification result, susceptibility is reported the result more become rationally, and clinical correct use microbiotic is had important directive significance.
The using method of this kit is: kind and the quantity of determining antibacterials according to the Bacteria Identification result.The reaction plate of choosing is installed on the square frame shape support, presses routine method operation (is example with every row's 12 reacting holes) then: with physiological saline bacterium liquid is corrected to 0.5 Maxwell than turbid standard, dilutes by 1: 200 with MH meat soup, making bacteria containing amount is 5 * 10
5CFU/ml inoculates 100 μ 1 at every reacting hole then.Make the final dilute concentration of every row's antibacterials be followed successively by 256,128,64,32,16,8,4,2,1,0.5,0.25,0.125 μ g/ml.With reaction plate concussion 1 minute, make that solution mixes each hole in, add the sealing of lid blended rubber paper and hatch the evaporation of moisture in the process with minimizing, put the interior 35C of wet box and hatched 16-20 hour.The result judges: according to the growth characteristic that detects bacterium and standard bacterium, become to occur at the bottom of muddy shape, the hole precipitation etc. as meat soup in the micropore and compare judgement, the lowest concentration of drug of no naked eyes visible growth is for measuring medicine to testing the MIC of bacterium.
Description of drawings
Fig. 1 is a structure principle chart of the present utility model;
Fig. 2 is the vertical view of Fig. 1.
In conjunction with the accompanying drawings the utility model is described in detail
The said square frame shape of the utility model support 2 is frameworks empty in, and this support 2 can be made into plurality of specifications such as 4 * 12 holes, 8 * 12 holes, 12 * 12 holes.One row or the reaction plate 3 more than the row are housed in this support 2, have row's V-arrangement reacting hole 4 on this reaction plate 3, the two ends of this reaction plate respectively have a fixed bridge 5, through this fixed bridge 5 reaction plate 3 is installed in this square frame shape support 2, in order to prevent water evaporates, a lid 1 also is housed on square frame shape support 2 constitutes.
Claims (1)
- The Microdilution susceptibility kit of dismantled and assembled combination, it comprises a square frame shape support (2), it is characterized in that: a row or the reaction plate (3) more than the row are housed in this support (2), have row's V-arrangement reacting hole (4) on this reaction plate (3), the two ends of this reaction plate (3) respectively have a fixed bridge (5), through this fixed bridge (5) reaction plate is installed in this square frame shape support (2), a lid (1) also is housed on square frame shape support (2) constitutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02214306 CN2544282Y (en) | 2002-04-26 | 2002-04-26 | Mountable/dismountable microquantity dilute drug sensitivity reagent box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02214306 CN2544282Y (en) | 2002-04-26 | 2002-04-26 | Mountable/dismountable microquantity dilute drug sensitivity reagent box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2544282Y true CN2544282Y (en) | 2003-04-09 |
Family
ID=33693663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02214306 Expired - Fee Related CN2544282Y (en) | 2002-04-26 | 2002-04-26 | Mountable/dismountable microquantity dilute drug sensitivity reagent box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2544282Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1990880B (en) * | 2005-12-27 | 2010-12-08 | 上海复星佰珞生物技术有限公司 | Non-zymocyte drug sensitive batten preparation method |
| CN103820310A (en) * | 2014-02-15 | 2014-05-28 | 江苏中新医药有限公司 | Concentration gradient detection kit for drug sensitivity tests and measuring method thereof |
| CN105219636A (en) * | 2015-10-19 | 2016-01-06 | 山东鑫科生物科技股份有限公司 | A kind of drug sensitive batten and preparation method thereof |
-
2002
- 2002-04-26 CN CN 02214306 patent/CN2544282Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1990880B (en) * | 2005-12-27 | 2010-12-08 | 上海复星佰珞生物技术有限公司 | Non-zymocyte drug sensitive batten preparation method |
| CN103820310A (en) * | 2014-02-15 | 2014-05-28 | 江苏中新医药有限公司 | Concentration gradient detection kit for drug sensitivity tests and measuring method thereof |
| CN103820310B (en) * | 2014-02-15 | 2017-11-07 | 江苏中新医药有限公司 | A kind of drug sensitivity test concentration gradient detection kit and method of testing |
| CN105219636A (en) * | 2015-10-19 | 2016-01-06 | 山东鑫科生物科技股份有限公司 | A kind of drug sensitive batten and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C56 | Change in the name or address of the patentee |
Owner name: YAN JING; LI GUOPING; QIN FUJI; WANG QIJIN Free format text: FORMER NAME OR ADDRESS: YAN JING; LI GUOPING; QIN FUJI |
|
| CP03 | Change of name, title or address |
Designer after: Yan Jing Designer after: Li Guoping Designer after: Qin Fuji Designer after: Wang Qijin Designer before: Yan Jing Designer before: Li Guoping Designer before: Qin Fuji |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |