CN2528018Y

CN2528018Y - ToRCH protein chip

Info

Publication number: CN2528018Y
Application number: CN 01263165
Authority: CN
Inventors: 唐祖明; 何农跃; 陆祖宏; 张春秀; 王宏; 梅茜; 朱毅
Original assignee: Nanjing Private Science And Technology Pioneering Center Co Ltd; Nanjing Jihui Science & Technology Co Ltd
Current assignee: Nanjing Private Science And Technology Pioneering Center Co Ltd; Nanjing Jihui Science & Technology Co Ltd
Priority date: 2001-10-11
Filing date: 2001-10-11
Publication date: 2002-12-25
Anticipated expiration: 2011-10-11

Abstract

The utility model discloses a ToRCH protein chip belonging to the technical field of biochip. The protein chip comprises a glass slide; a macromolecule xylanase membrane is adhered to the glass slide; the surface of macromolecule xylanase membrane is adhered with a ToRCH antigen-antibody protein microarray. All ToRCH diagnosis can be completed by a chip at one time in a short time of about 2 hours, which is very convenient and fast; the cost is much lower than that of the examination of imported kit. Therefore, as the utility model is released, the popularity of ToRCH disease examination and diagnosis will be promoted, which will have vital and far-reaching significance for the good prenatal and postnatal care and the improvement of the quality of newborn population in China, even in the entire world.

Description

The ToRCH protein-chip

Technical field

The utility model relates to a kind of protein-chip, and especially a kind of protein-chip of diagnosing usefulness belongs to the biochip technology field.

Background technology

Nineteen nineties, a brand-new era is brought biotechnology in biochip research at the very start.Compare with the genetic chip that causes extensive concern, protein-chip can reflect the protein level in the biosome, therefore can express more complete biological information.

" ToRCH " is the technical term of modern perinatal medicine.Wherein, T represents toxoplasma gondii (Toxoplasmagondii), O represents (Other) such as Epstein-Barr virus, HIV virus and human parvovirus B19s, R represents rubella virus (Rubella Virus), C represents cytomegalovirus (Cytomegalovirus), and H represents herpes simplex I type and II type virus (Herpes Simplex Virus I and II).The scope of these pathogenic infections is wide, harm is big, and the pregnant woman is easy to take place primary infection, causes miscarriage, premature labor, stillborn foetus or growth retardation of fetus and developmental deformity, thus the population quality of having a strong impact on.

Developed countries such as America and Europe just classify it as pregnancy period examination project as far back as the seventies.Pregnant earlier T oRCH testing, especially Beijing have also progressively been carried out in China developed regions, and municipal government has issued the relevant document of ToRCH being classified as pregnant woman's routine inspection project.

At present, the complete dependence on import of kit of China ToRCH deagnostic test, it costs an arm and a leg, because the every box reagent of kit need use up in the short as far as possible time, therefore requires the number of inspection simultaneously and the dosage coupling of every box reagent.The person under inspection need spend 300-500 unit, usually will wait for that there is the result in several old name for the Arabian countries in the Middle Easts.Expensive inspection charge and loaded down with trivial details scrutiny program have limited popularizing that ToRCH checks to a great extent.

The purpose of this utility model is: at the problem of above-mentioned existing ToRCH deagnostic test reagent existence, utilize the high-tech biotechnology to propose a kind of ToRCH protein-chip, thereby the diagnosis that makes the ToRCH disease has more science, checks the diagnostic means method easily, can generally accept for hospital, patient.

In order to reach the foregoing invention purpose, the technical solution of the utility model is: the ToRCH protein-chip comprises microslide, and described microslide is attached with macromolecule glycan film, and described macromolecule glycan film surface attachment has ToRCH antigen-antibody protein microarray.

During inspection, be coated on clearly on the chip of the present utility model as long as will wait to have a blood test, if the person under inspection is by the ToRCH virus infections, its serum will be very fast with chip on corresponding antigen-antibody generation idiosyncrasy, when by fluorescence microscope, just show corresponding positive feature, like this in the short time about two hours, just can finish all ToRCH diagnosis by a slice chip is disposable, very convenient quick, its cost is significantly less than the expense that the import reagent box is checked.Therefore, after technology of the present utility model is released, will greatly promote popularizing of ToRCH disease examination, to the prenatal and postnatal care and the important and far-reaching meaning of raising newborn population quality generation in the China and even the world.

Description of drawings

Below in conjunction with accompanying drawing the technical solution of the utility model is described further.

Fig. 1 is the structural representation of technical solutions of the utility model embodiment one.

Embodiment

Embodiment one

The ToRCH protein-chip of present embodiment adopts glass as microslide 1, the upper surface of microslide 1 is attached with one deck agarose film 2, the upper surface point of agarose film 2 has ToRCH antigen protein microarray 3, and point has ToRCH antibody 4 (referring to Fig. 1) on the ToRCH antigen microarray 3.

Embodiment two

The ToRCH protein-chip of present embodiment adopts silicon chip as microslide 1, the upper surface of microslide 1 is attached with one deck glucosan---polyacrylamide film 2, the upper surface point of glucosan---polyacrylamide film 2 has ToRCH antigen protein microarray 3, and point has ToRCH antibody 4 on the ToRCH antigen microarray 3.

During inspection, be coated on clearly on the chip as long as will wait to have a blood test, in short time about two hours, just can finish all ToRCH diagnosis by a slice chip is disposable, very conveniently fast experimental results show that, its sensitivity and specificity are all very good, and its cost only is 30% of an import reagent box inspection charge.Therefore, after the release, will have very vast market prospect, have a tremendous social and economic benefits.

In addition to the implementation, the technical solution of the utility model also has other numerous embodiments.For example, microslide adopts Ge, GaAs, GaP, SiO2, Si3N4 sheet, or gel, glycan thing such as poly-tetrafluoro, poly-alum, polycarbonate; Macromolecule glycan film is an agarose---polyacrylamide base film or agarose---glucosan---polyacrylamide base film etc.; All employings are equal to the technical scheme of replacement or equivalent transformation formation, are the protection domain of the utility model requirement.

Claims

1. a ToRCH protein-chip comprises microslide, it is characterized in that: described microslide is attached with macromolecule glycan film, and described macromolecule glycan film surface attachment has ToRCH antigen-antibody protein microarray.

2. ToRCH protein-chip according to claim 1 is characterized in that: described macromolecule glycan film upper surface point has ToRCH antigen protein microarray, and point has ToRCH antibody on the ToRCH antigen microarray.

3. ToRCH protein-chip according to claim 1 and 2 is characterized in that: described microslide is a glass, and the upper surface of described glass slide is attached with one deck agarose film.

4. ToRCH protein-chip according to claim 1 and 2 is characterized in that: described microslide is a silicon chip, and the upper surface of silicon chip microslide is attached with one deck glucosan---the polyacrylamide film.

5. according to claim 2 or 3 described ToRCH protein-chips, it is characterized in that: described microslide adopts Ge, GaAs, GaP, SiO2, Si3N4 sheet, or the gel glycan thing of poly-tetrafluoro, poly-alum, polycarbonate.

6. ToRCH protein-chip according to claim 1 is characterized in that: described macromolecule glycan film is an agarose---polyacrylamide film or agarose---glucosan---polyacrylamide film.