CN2466647Y

CN2466647Y - Probe capable of measuring plant cell pressure and cell membrane potential simultaneously

Info

Publication number: CN2466647Y
Application number: CN 01203813
Authority: CN
Inventors: 朱果利
Original assignee: BIOLOGY COLLEGE CHINESE AGRICULTURE UNIV
Current assignee: BIOLOGY COLLEGE CHINESE AGRICULTURE UNIV
Priority date: 2001-02-23
Filing date: 2001-02-23
Publication date: 2001-12-19
Anticipated expiration: 2011-02-23

Abstract

The utility model relates to a probe capable of measuring plant cell pressure and cell membrane potential simultaneously. An acicular glass wool pipe is provided with a fine glass thread which is formed into integration with the glass wool pipe. Sintered both sides of the fine glass thread and the glass wool pipe are attached with conductive potassium chloride solution in water, and the whole glass wool pipe is filled with medium oil. The utility model has the advantages of simple structure, and can simultaneously measure the plant cell turgor pressure and the cell membrane potential, and is convenient and accurate.

Description

Can measure the probe of vegetable cell pressure and cell membrane potential simultaneously

The utility model relates to a kind of probe that can measure vegetable cell pressure and cell membrane potential simultaneously.

Later stage in the sixties, German E.Steudle has invented a kind of probe that can directly measure the vegetable cell turgescence, the vegetable cell turgescence is one of important physiological parameter of plant cytology, this instrument has promoted the development of modern plants stechiology (referring to No. 2745437, Deutsche Bundespatent, July 10 in 1980 applying date order, inventor Enrst Steudle), its structure as shown in Figure 1, a glass hollow billet A is by stationary pipes B, sealing gasket C is equipped with screw micrometer D in its back-end, the front end of described glass hollow billet A is made pointed, on its pipe sidewall pressure transducer E is housed, glass hollow billet inside is full of oily medium F, when the glass fiber tip end of this cell pressure probe thrusts the vacuole of vegetable cell G, liquid bag liquid enters glass fiber tip end inside under the turgescence effect, because water oil is immiscible, so in the glass hollow billet, form water-oil interface H, cell turgor is under the mediation of liquid medium (vacuolar sap and the oil that is full of glass hollow billet inside), reach pressure transducer E and perceived, its pressure can change by adjustable screw milscale D.Thereby realize the position adjustments of water-oil interface H, and then implement mensuration the water conductance.Cell membrane potential be one than turgescence even more important and responsive and with the associated physiological parameter of turgescence, can measure the turgescence of individual cells and cell membrane potential simultaneously with a probe enlarges important physiological function such as growth very big meaning is arranged to the research cell, and at present because during the pressure measurement of cell pressure probe, it must be full of oily medium in its needle-like glass capillary and can not the conduct electricity signal, Gu and can not measure cell pressure and cell membrane potential simultaneously.

The purpose of this utility model just provides a kind of probe that can measure vegetable cell pressure and cell membrane potential simultaneously.

For achieving the above object, the utility model is taked following design proposal: being shaped on one in needle-like glass hollow billet is the thin glass yarn of one with the glass hollow billet, has the potassium chloride solution that conducts electricity in the both sides of clinkering at described thin glass yarn mutually with the glass hollow billet.And and be filled with medium oil in the whole glass hollow billet.Here it is a kind of probe that can measure vegetable cell pressure and cell membrane potential dual signal simultaneously.

The utility model can be made like this, sintering one glass fiber in a glass hollow billet, molten drawing forms the glass hollow billet that its inside has thin glass yarn, in the glass hollow billet, charge into potassium chloride solution, pour out then, because glass surface is hydrophilic, more determine potassium chloride solution to tend to be adsorbed on firmly the trickleer capillary space between glass hollow billet and the sintering filaments of glass thereon by thermodynamic principles, when charging in the glass hollow billet with oily medium, being present in the trickleer capillary space of thin glass yarn and glass hollow billet is that the conduction Klorvess Liquid that the molten mutually both sides of cutting of thin glass yarn and glass hollow billet have can not substituted by oily medium again.In whole glass hollow billet, just there is a conductive channel like this, when this probe tip penetrates into the vegetable cell vacuole, oily medium in vacuolar sap and the glass hollow billet forms oil-water interface, satisfy all requirements of pressure measurement of conventional cell pressure probe and water conductance, simultaneously owing to exist conductive channel can measure film potential simultaneously again in the glass hollow billet.

The utility model is simple in structure, can measure vegetable cell turgescence and cell membrane potential simultaneously, and is convenient and accurate.

The utility model is described in further detail below in conjunction with accompanying drawing.

Fig. 1 is the existing probe of measuring the vegetable cell turgescence

Fig. 2 is the utility model probe structure synoptic diagram

Fig. 3 is the utility model probe application principle figure

Embodiment

As shown in Figure 2, in the glass hollow billet 1 of a needle-like, clinkering has a thin glass yarn 2, has the potassium chloride solution 4 that conducts electricity in the both sides of clinkering at described thin glass yarn mutually with the glass hollow billet, and be filled with medium oil 3 in the whole glass hollow billet, its principle of work as shown in Figure 3, the turgescence of cell by the conduction of the medium oil 3 in the glass hollow billet by pressure transducer 5 perception and be converted to electric signal, cell vacuolar sap 13 is at the inner water oil interface 6 that forms of glass fiber tip, the position at this interface can be put milscale 7 by spiral shell and regulated, to realize the mensuration of conventional cell pressure probe to turgescence and water conductance, the cell membrane potential signal is taken out from cell by probe tip, pass to silver chloride electrode 8 in the potassium chloride solution of normal concentration, reference electrode 9 in this electrode and the extracellular fluid, the Klorvess Liquid 11 of normal concentration, silver chloride electrode 10 forms the loop, and its voltage drop is predicted by voltage table 12.

Claims

1, a kind of probe that can measure vegetable cell pressure and cell membrane potential simultaneously, it is characterized in that: being shaped on one in needle-like glass hollow billet is the thin glass yarn of one with the glass hollow billet, have the potassium chloride solution of conduction in the both sides of clinkering at described thin glass yarn mutually with the glass hollow billet, and in whole glass hollow billet, be filled with medium oil.