CN2455262Y

CN2455262Y - Microinjection device with injection pressure determination

Info

Publication number: CN2455262Y
Application number: CN 00265438
Authority: CN
Inventors: 陈�峰
Original assignee: Individual
Current assignee: Individual
Priority date: 2000-12-27
Filing date: 2000-12-27
Publication date: 2001-10-24
Anticipated expiration: 2010-12-27

Abstract

The utility model relates to a microinjection device with the checking function of injecting pressure, which is used for a microinjection technology, wherein, a hydraulic cylinder is connected with a pressure sensor and a microinjection piston in a gap space without movable fit. The structure realizes that the residual gas of micro quantity in the hydraulic cylinder is removed when hydraulic medium is filled and poured. The hydraulic medium has no elastic deformation in microinjection. The change and the degree of the injecting pressure can be obtained to obtain the purposes of setting the tolerance limit of an injecting object and reducing the consumption of the injecting object. The structure of a cylinder body is easy to be disassembled and is helpful for the cleaning and disinfecting treatment in the hydraulic cylinder.

Description

The microinjection instrument that possesses the injection pressure measuring ability

Microinjection instrument is to implement the necessary instrument of microinjection technique, be the structure of representative with patent ZL99228317.5 in the prior art, though prevent that hydraulic medium from leaking and serviceability on more in the past technology, as as the micro-notes device of components of stres improvement being arranged with injector for medical purpose, but find in actual use: the bubble that (1) still has the very little human eye of a little volume to discern after charging hydraulic medium is distributed in the hydraulic medium, the change of volume can take place with the change of pressure medium in these bubbles, thereby influence the susceptiveness and the accuracy of microinjection, trace it to its cause, discovery be in the gap that is slidingly matched between microinjection piston 6 and casing wall 6-1 remaining gas do not discharge cause, as shown in Figure 1, this section is slidingly matched and has formed narrow space, after charging into medium in the vestibule 4-1, medium can promptly overflow in spacious space 5-6 and be full of, when medium enters this section narrow space, because capillarity is slowed down velocity of medium, the path of being discharged by medium shutoff gas during underfill still, thereby these gases that remain in the medium form bubble, and this is the fault of construction that exists in the prior art.(2) the unsolved problem of prior art is when being injected into the material in the microinjection pin in the target object, can't know the pressure of injection, burst apart for injection mass promptly being injected in the target object simply intensified pressure so that injecting object, cause the loss of injection object big, because object mostly is cultured cell, to drop into suitable financial resources and manpower in the preparation, therefore be necessary when operation, to understand the change of injection pressure and the proof pressure limit that size is come the target setting thing, this will help the saving of object, fail to accomplish to know the injection pressure parameter in the prior art, therefore be necessary to improve.(3) in some environments for use that hygienic state is had relatively high expectations, as require aseptic or sterilization, need carry out clean to the media Containers of syringe, its requirement can be convenient to decompose syringe so that the cleaning medium container, as hydraulic cylinder etc., obviously prior art is difficult, and inconvenience is decomposed, dismounting, also need improve.

The purpose of this utility model provides a kind of microinjection instrument that possesses the injection pressure measuring ability, it not only can promptly charge hydraulic medium and get rid of bubble and have the function of knowing syringe internal pressure parameter, can be convenient to the media Containers of syringe is decomposed, to reach accurate sensitive injection performance, understand the purpose of the media Containers of injection pressure size and syringe convenient for cleaning at any time.

The purpose of this utility model is to realize like this.A first cylindricality cylinder body and an end of being made up of two various outer diameters on less external diameter is set external screw thread is set, cylinder body is horizontal positioned, a through hole of being made up of three sections different inner diameters is set in cylinder body, begin to be followed successively by pick off from cylinder body one end screw, dark hole and bright hole are installed near big external diameter, three holes are coaxial, and the diameter proportion in three holes was followed successively by 4: 3: 1; In pick off is installed screw, placed the O RunddichtringO and be connected with a pressure transducer, pressure transducer send display to be connected with change, on the upside cylinder wall of dark hole section, be provided with the hose coupling screw and the hydraulic medium that are connected with the microinjection needle cannula and charge screw, two screw axis be parallel to each other and with the cylinder body axis normal, the aperture upwards and with dark hole communicates, in two screws, place the O RunddichtringO, hose coupling screw and hose coupling link, hydraulic medium charges screw and is connected with sealed screw, two windows are set on the cylinder wall of bright hole section, two one of windows be positioned at the cylinder body axis directly over, another is under the cylinder body axis, and the length of window is threaded into the length at two external diameter step places less than the outer unit of cylinder body end; Place a glass-tube O RunddichtringO first in bright hole with the step place that intersect in dark hole, in bright hole, also place a transparent glass tube, transparent glass tube and cylinder body are coaxial, glass tube outer diameter and bright bore dia are shape movingly, internal diameter contacts greater than dark bore dia and with glass-tube O-ring seals first in being placed on bright hole, the other end of transparent glass tube contacts with another glass-tube O-ring seals second, glass-tube O-ring seals second contacts with the cylindrical step of a cylindrical guide pin bushing, the cylindrical of three sections different-diameters is set on the cylindrical of cylindrical guide pin bushing, one section diameter is identical with bright bore dia wherein, the diameter of left and right sides two-stage nitration cylindrical is identical with the external diameter of transparent glass tube, therefore two steps about the intersection of one section cylindrical of left and right sides two-stage nitration cylindrical and centre forms, the endoporus of guide pin bushing is made up of two-stage nitration, one section is straight hole, another section is funnel-shaped apertures, the aperture of funnel-shaped apertures is positioned on the end face of guide pin bushing one side, the axis of this end face and guide pin bushing is perpendicular, and the crossing intersection of end face that is cone shape inclined-plane and guide pin bushing one side between the big aperture of funnel-shaped apertures forms the aperture of funnel-shaped apertures; Intersect the macropore that forms funnel-shaped apertures with straight hole, the aperture of funnel-form endoporus was near transparent glass tube when cylindrical guide pin bushing contacted with O-ring seals second, the straight hole section is away from transparent glass tube, it is coaxial that back guide pin bushing and cylinder body are installed, another cylindrical step of cylindrical guide pin bushing contacts with at the bottom of the screw of a fastening cap, be provided with the endoporus of two different-diameters on the fastening cap, two endoporus and cylinder body are coaxial, one of them endoporus is that its diameter of screw is identical with the cylinder body outside nut, another endoporus is a straight hole, on the cylindrical of this straight hole, be provided with external screw thread, the inner bolt hole of fastening cap is connected with the cylinder body external screw thread, and fastening cap and cylinder body are coaxial, tightens and can make guide pin bushing extruding glass-tube O RunddichtringO second behind the fastening cap, and then, make the end face of transparent glass-tube and cylindrical guide pin bushing be in sealing state by transparent glass-tube extruding glass-tube O-ring seals first; In the endoporus of guide pin bushing, place a microinjection piston, the aperture of the funnel-shaped apertures of piston and guide pin bushing is joins state, the ratio of the external diameter of piston and the internal diameter of transparent glass-tube is 1: 2-1: between 4, the cylindrical of piston is made of smooth section and thread segment, wherein smooth section penetrates the aperture of guide pin bushing funnel-shaped apertures and partly enters in the transparent glass tube, on the cone shape inclined-plane of the funnel of guide pin bushing endoporus, place O-ring seals, O-ring seals is enclosed within on the piston smooth section, O-ring seals contacts with an ejector sleeve that is enclosed within on the outside piston, the cylindrical of two different-diameters is set on the ejector sleeve, the outside diameter of close O-ring seals section and identical being movingly of straight hole diameter of conduit, its length surpasses the length of guide pin bushing straight hole, identical being movingly of straight hole diameter of the outside diameter of another section and fastening cap, and when ejector sleeve contacts with the piston O-ring seals, the outside nut end face that the other end of ejector sleeve stretches out fastening cap contacts with at the bottom of the female screw hole of a And medicated cap, the female screw hole of And medicated cap is connected with the outside nut of fastening cap, piston passes the endoporus of And medicated cap, And medicated cap and cylinder body are coaxial, after tightening the And medicated cap, ejector sleeve extrusion piston O-ring seals makes between the coniform inclined-plane of funnel-shaped apertures of outside piston and conduit and is sealing state, after said structure is formed, pick off is installed screw, dark hole, transparent glass tube, the perpendicular end surface of cylindrical guide pin bushing, the microinjection piston constitutes hydraulic medium container or hydraulic cylinder, contacting between the aperture of piston and the funnel-shaped apertures of guide pin bushing is line and the contacting of face, therefore, in hydraulic cylinder, there is not interstitial structure movingly between face and the face; The screw thread of piston is connected with the female screw hole of a screw mandrel, and screw mandrel passes hold-down nut and is connected with handwheel, and hold-down nut is connected with the vertical component of support, and cylinder body is connected by screw with the support level part, and the support level part is connected with pedestal; During use, unload medium and charge sealed screw on the hole, after the injector for medical purpose mouth that has charged hydraulic medium inserted medium and charge O-ring seals contact in the Kong Bingyu hole, medium is injected, because the cylinder body axis is the level of state, medium enters in the hydraulic cylinder its horizontal plane of back and rises gradually and cover the cylinder body central axis earlier, gas in the cylinder barrel is ejected by the hose coupling place, directly over the WATER AS FLOW MEDIUM plane soon taps into transparent glass tube during inwall, pick off one end perk a little with support, continue to charge medium, so that remaining gas is discharged from hose coupling in the cylinder, when medium stops to charge after flexible pipe flows out, and support recovered horizontal positioned, if when also having gas in the cylinder, can observe from the top window of glass-tube, tilt stand continues to charge medium a little again, it is remaining to repeat aforesaid operations no gas in confirming cylinder, take off injector for medical purpose, this moment is because the horizontal plane of flexible pipe medium is higher than the horizontal plane in the cylinder barrel, medium in the flexible pipe will slowly reflux in the cylinder, medium can diffuse out from charge screw, rapidly sealed screw is screwed in screw, can avoid air to bring medium into, when microinjection, the cylinder inner volume is dwindled generation pressure when the precession of rotation hand wheel driven plunger, by with flexible pipe that the microinjection needle cannula links to each other in hydraulic medium transfer the pressure to needle tubing so that material is expelled in the objective body; Feel the change of pressure and send display to know with the contacted pressure transducer of hydraulic medium, can determine the critical withstand voltage limit of object by experiment, thereby play the purpose of protection object by change; When the needs clean injectors, be connected and cylinder body and support level connecting screw partly with cylinder body is externally threaded as long as unclamp fastening cap, take off cylinder body and make cylinder barrel inside carry out cleaning and disinfection.

Adopt the scheme of above-mentioned utility model to have following positive effect and performance:

One. hydraulic medium can promptly fill and overflow in the hydraulic cylinder institute and have living space and gas is discharged in hydraulic cylinder, thus the nonelastic distortion of hydraulic medium can guarantee microinjection the time, thus the susceptiveness and the accuracy of injection improved.

Two. show now that by pressure transducer and corresponding data system can in time understand the change of injection cylinder internal pressure, reach protection injection object and reduce the purpose of the raw-material loss of experiment.

Three. can reach and be convenient to the purpose dismantling cylinder body, be convenient to cleaning and disinfection cylinder barrel inside.

Below in conjunction with drawings and Examples this utility model is further specified.

Fig. 1 is the longitudinal structure figure of prior art.

Fig. 2 is this utility model longitudinal profile structural map.

In the embodiment shown in Figure 2, a first cylindricality cylinder body 1 and an end of being made up of two various outer diameters on less external diameter is set external screw thread 12 is set, unit's cylindricality cylinder body 1 is horizontal positioned, a through hole of being made up of three sections different inner diameters is set in cylinder body 1 begins to set gradually pick off installation screw 2, dark hole 3 and bright hole 4 from cylinder body one end near big external diameter; In pick off is installed screw 2, placed O RunddichtringO 2-1 and be connected with the sheath 5-1 of a pressure transducer 5,3 sections cylinder body axis upside is provided with that the microinjection needle cannula connects hose coupling screw 6 and hydraulic medium charges screw 7 in dark hole, two screw axis be parallel to each other and with the cylinder body axis normal, the aperture upwards, in two screws, place O RunddichtringO 6-1, hose coupling screw 6 links with hose coupling 8, hydraulic medium charges screw 7 and is connected with sealed screw 7-1, two window 9-1 are set on the cylinder wall of bright hole section, 9-2, window 9-1 be positioned at the cylinder body axis directly over, window 9-2 is under the cylinder body axis, place at bright hole 4 and step 13 places in dark hole 3 a glass-tube O RunddichtringO first 14-1 and with in bright hole 4, also place a transparent glass tube 15 and contact, the other end of transparent glass tube 15 is connected with another glass-tube O-ring seals second 14-2, glass-tube O-ring seals second 14-2 contacts with the cylindrical step of a cylindrical guide pin bushing 16, another cylindrical step of cylindrical guide pin bushing 16 contacts with at the bottom of the screw of a fastening cap 11, the inner bolt hole of fastening cap 11 is connected with cylinder body external screw thread 12, can make guide pin bushing 16 extruding glass-tube O RunddichtringO second 14-2 after tightening fastening cap 11, and then by transparent glass-tube 15 extruding glass-tube O-ring seals first 14-1, make transparent glass-tube 15 and dark hole 3, guide pin bushing 16 is in sealing state; In the endoporus of guide pin bushing 16, place a microinjection piston 18, aperture and part that the smooth section of outside piston is passed the guide pin bushing funnel-shaped apertures enter in the transparent glass tube 15, on the funnel-form inclined-plane of aperture 16-1, place O-ring seals 19, O-ring seals 19 is enclosed within on the piston 18, O-ring seals 19 contacts with an end face that is enclosed within the ejector sleeve 20 on piston 18 cylindricals, the outside nut end face that ejector sleeve 20 other ends stretch out fastening cap 11 contacts with at the bottom of the female screw hole of a And medicated cap 21, the female screw hole of And medicated cap 21 is connected with the outside nut of fastening cap 11, piston 18 passes the endoporus of And medicated cap, after tightening And medicated cap 11, O-ring seals 19 on ejector sleeve 20 extrusion pistons 18 makes between the inclined-plane 16-1 of funnel-shaped apertures of piston 18 cylindricals and conduit and is sealing state, after said structure is formed, pick off 5, dark hole 3, transparent glass tube 15, cylindrical guide pin bushing 16, piston 18 constitutes hydraulic medium container or hydraulic cylinder, hydraulic medium 10 is distributed in the hydraulic cylinder, the thread segment of piston 18 is connected with the female screw hole of a screw mandrel 22, screw mandrel 22 passes hold-down nut 23 and is connected with handwheel 24, hold-down nut 23 is connected with the vertical component 25-1 of support, cylinder body 1 is connected by screw 26 with support level part 25-2, and support level part 25-2 is connected with pedestal 17.

Claims

1, a kind of microinjection instrument that possesses the injection pressure measuring ability, a first cylindricality cylinder body and an end of being made up of two various outer diameters on less external diameter cylinder body is set external screw thread is set, cylinder body is horizontal positioned, it is characterized in that:, in cylinder body, a through hole of being made up of three sections different inner diameters is set and begins to be followed successively by pick off installation screw from cylinder body one end near big external diameter along its axis direction, dark hole and bright hole, three holes are coaxial, in installing screw, pick off placed the O RunddichtringO, pick off is installed screw and is connected with a pressure transducer, pressure transducer send display to be connected with change, on cylinder wall directly over the axis of dark hole section, be provided with microinjection needle cannula connection hose coupling screw and hydraulic medium and charge screw, two screw axis be parallel to each other and with the cylinder body axis normal, the aperture upwards, in two screws, place the O RunddichtringO, hose coupling screw and hose coupling link, hydraulic medium charges screw and is connected with sealed screw, place a glass-tube O RunddichtringO first in bright hole with the step place that intersect in dark hole, O RunddichtringO first with in bright hole, also place a transparent glass tube and contact, the other end of transparent glass tube is connected with another glass-tube O-ring seals second, glass-tube O-ring seals second is connected with the cylindrical step of a cylindrical guide pin bushing, another cylindrical step of cylindrical guide pin bushing contacts with at the bottom of the screw of a fastening cap, the inner bolt hole of fastening cap is connected with the cylinder body external screw thread, in the endoporus of guide pin bushing, place a microinjection piston, on the cone shape inclined-plane of the funnel of guide pin bushing endoporus, place O-ring seals, O-ring seals is enclosed within on the piston smooth section, O-ring seals is connected with an ejector sleeve that is enclosed within on the outside piston, the outside nut end face that the other end of ejector sleeve stretches out fastening cap contacts with at the bottom of the female screw hole of a And medicated cap, the female screw hole of And medicated cap is connected with the outside nut of fastening cap, the screw thread of piston is connected with the female screw hole of a screw mandrel, screw mandrel passes hold-down nut and is connected with handwheel, hold-down nut is connected with the vertical component of support, cylinder body is connected by screw with the support level part, the support level part is connected with pedestal, and pressure transducer send display to be connected with change.

2, the microinjection instrument that possesses the injection pressure measuring ability according to claim 1, it is characterized in that: the endoporus of cylindrical guide pin bushing is made up of two-stage nitration, one section is straight hole, another section is funnel-shaped apertures, the aperture of funnel-shaped apertures is positioned on the end face of guide pin bushing one side, and the axis of this end face and guide pin bushing is perpendicular, and the crossing intersection of end face that is cone shape inclined-plane and guide pin bushing one side between the big aperture of funnel-shaped apertures forms the aperture of funnel-shaped apertures; Intersect the macropore that forms funnel-shaped apertures with straight hole.

3, the microinjection instrument that possesses the injection pressure measuring ability according to claim 1, it is characterized in that: two windows are set on the cylinder wall of bright hole section, two one of windows be positioned at the cylinder body axis directly over, another is under the cylinder body axis, and the length of window is threaded into the length of the crossing step of two outside diameters of cylinder body less than the outer unit of cylinder body end.

4, the microinjection instrument that possesses the injection pressure measuring ability according to claim 1 is characterized in that: the external diameter of transparent glass tube and bright bore dia are shape movingly, and internal diameter is greater than dark bore dia.

5, the microinjection instrument that possesses the injection pressure measuring ability according to claim 1 is characterized in that: the ratio of the external diameter of microinjection piston and the internal diameter of transparent glass tube is 1: 2-4: between 1.

6, the microinjection instrument that possesses the injection pressure measuring ability according to claim 1 is characterized in that: pick off is installed screw, dark hole and bright hole, and the diameter proportion in three holes was followed successively by 4: 3: 1.