CN2447788Y - Self-setting capillary electrophoretic electrochemical detecting tank - Google Patents
Self-setting capillary electrophoretic electrochemical detecting tank Download PDFInfo
- Publication number
- CN2447788Y CN2447788Y CN 00218805 CN00218805U CN2447788Y CN 2447788 Y CN2447788 Y CN 2447788Y CN 00218805 CN00218805 CN 00218805 CN 00218805 U CN00218805 U CN 00218805U CN 2447788 Y CN2447788 Y CN 2447788Y
- Authority
- CN
- China
- Prior art keywords
- electrode
- detecting
- capillary
- self
- utility
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a self-setting capillary electrophoretic electrochemical detecting tank. The utility model changes the mode that the traditional mode has poor repeatability on manual adjustment of the relative position of a capillary outlet and detecting electrodes by a three-position orientator. The utility model ensures that the capillary outlet and the detecting electrodes sealed in an electrode hole are reliably aligned by a capillary guiding hole and the detecting electrode hole which are arranged on the same insulation material in a coaxial aligning way. As a consequence, the utility model improves detecting reproducibility and accuracy. Meanwhile, the utility model reduces the volumes of a positioning device and a detecting device and lowers production cost and material consumption.
Description
The utility model relates to a kind of capillary electrophoresis electrochemical detection technique, refers to a kind of capillary pipe electrophoresis electrochemistry detecting pool that can be self-align especially.
Capillary Electrophoresis (Capillary electrophoresis, i.e.CE) similar with high performance liquid chromatography, be a kind of separation detection technology, be a kind of new-type instruments analytical technology in the analytical chemistry.Its purposes is that each component in the analytic sample (as the glucose in the serum, uric acid etc.) is passed through electrophoretic separation in kapillary, is detected then also quantitatively.Capillary Electrophoresis is that particle is a driving force with the electric field, in kapillary by its mobility (the average electrophoretic velocity of ion under the unit electric field) or the partition factor difference is carried out efficiently, a kind of electrophoresis new technology of separating fast.
Typical detection cell design and locating device are with the three-dimensional regulation device disk electrode to be passed through manual adjustment as shown in Figure 1 in the present existing capillary electrophoresis electrochemical detection technique, are alignd with capillary outlet in the disk electrode surface.Because the reappearance of each manual adjustment is relatively poor, accuracy and precision that influence is measured.And this device volume is bigger, and shock resistance is bad, and slight vibrations will cause the subtle change of disk electrode and capillary outlet relative position, and so accuracy and precision that influence is measured are must be very careful when this device of operation.
The purpose of this utility model is to provide a kind of reappearance height, self-align capillary pipe electrophoresis electrochemistry detecting pool simple to operation.
Self-align capillary pipe electrophoresis electrochemistry detecting pool of the present utility model includes pre-determined bit capillary electrophoresis electrochemical detecting electrode and electrode suppor, electrolytic cell, electrolytic solution, auxiliary electrode and ground-electrode (being two platinum filaments), contrast electrode, connector, wherein pre-determined bit capillary electrophoresis electrochemical detecting electrode comprises the electrode suppor of the insulator that is used to install kapillary and detecting electrode, as can be the organic glass support, be used to detect the garden disc electrode of measured object, the kapillary bullport that is used for the insulated copper lead of current signal transmission and is used to guide capillary outlet and test disks electrode collimation.The electrode suppor of making by transparent insulation material, be at a slice square or rectangle transparent insulation material thin slice, it is the electrolytic solution via that a rectangular opening is left at the middle part, be used for conducting electrolytic solution, observe the relative position of capillary outlet and test disks electrode and handle the detecting electrode surface, on rectangular opening dielectric sheet material up and down, get out the circular hole of two internal diameter 400-500 μ m then by the machining of precision with drill bit, because the quartzy fusion external diameter capillaceous that Capillary Electrophoresis is used is 360 μ m, wherein wearing hole capillaceous internal diameter requirement is 400 μ m, this hole is called the kapillary bullport, require two holes to become line with one heart, it is the key of finished product detection electrode and capillary outlet collimation.One of them penetrates the electrode that is connected with the contact conductor that has insulation course, electrode material is generally carbon-point, copper wire, platinum filament, spun gold, nickel wire of diameter 300-500 μ m etc., make electrode fix and make electrode and electrolytic solution insulation with glue (as the chloroformic solution of 502 glue or polymethylmethacrylate) sealing, wherein electrode passes electrode hole 1-1.5mm, insulate with glue on every side, radially electrode is worn into discoidly with file, be used for the electrochemical detection electrode disc surfaces and align with capillary outlet.The fixed block that lastblock is made with rubber or plastic insulation elastic body is glued after leaving guiding groove in kapillary bullport upper end, have in the middle one with dielectric sheet material on the concentric aperture of kapillary bullport, internal diameter is less than the kapillary external diameter, after kapillary inserts, be used for fixing kapillary, and function of seismic resistance arranged to improve the reappearance that detects.Kapillary bullport and electrode hole are the coaxial lines that becomes, and the disk detecting electrode in being sealed in electrode hole and kapillary also are coaxially to become line, thus the test disks capillary outlet align reliably with disk electrode, to improve the reappearance of location.
Pre-determined bit capillary electrophoresis electrochemical detecting electrode is the degree of closeness by the fixed block adjusting that is bonded in the insulating elastomer on the detecting electrode support and fixed capillary outlet and disk detecting electrode surface.
Electrode suppor is for can insert the mobile that also can take out in the detection cell.
The self-align capillary pipe electrophoresis electrochemistry detecting pool of the utility model top capping is provided with two electrode terminals and patchhole capillaceous, ground-electrode patchhole with the contrast electrode hole of inserting contrast electrode and at least more than one lead patchhole and insertion ground-electrode.
The self-align capillary pipe electrophoresis electrochemistry detecting pool of the utility model is owing to leave guiding bullport capillaceous and fixed block on the pre-determined bit capillary electrophoresis electrochemical detecting electrode support that uses, kapillary can be aimed at the disk detecting electrode, do not need to use the three-dimensional regulation device to regulate electrode and capillary outlet relative position, improved the reappearance of capillary outlet and detecting electrode collimation effectively, easy and simple to handle, the design with the supporting electrolytic tank of pre-determined bit capillary electrophoresis electrochemical detecting electrode, volume is little, reagent consumes little, be easy to shielding, reduced the volume of capillary electrophoresis apparatus simultaneously, help the integrated and miniaturization of capillary electrophoresis electrochemical detector, simple in structure small and exquisite, shock resistance is good, be easy to produce and safeguard, employing totally-enclosed design reduces the influence of effluve, improves and measures sensitivity, detecting electrode on the pre-determined bit capillary electrophoresis electrochemical detecting electrode is positioned at the electrolytic solution via of electrode suppor, is difficult for pickup and damages.
The self-align capillary pipe electrophoresis electrochemistry detecting pool of the utility model not only can be used for capillary electrophoresis electrochemical and detects (comprise ampere, electricity is led and current potential detects), also can be used for the electrolytic cell in the electrochemical gagings such as voltammetry, ampere method, and also can be used as the detecting device in the flow injection Electrochemical Detection.
Now further specify the utility model in conjunction with the accompanying drawings
Symbol description
1-pre-determined bit capillary electrophoresis electrochemical detecting electrode, 2-electrolytic solution, 3-garden dish detecting electrode, 3 '-ground-electrode, 3 "-auxiliary electrode, 4-insulated conductor; 5-electrode terminal, 6-fixed block, 7-electrolytic solution via; 9-kapillary, 13-ground-electrode hole, 14-contrast electrode hole; 18-wire guide, 19-kapillary bullport, 19 '-bottom kapillary bullport; 8-three-dimensional locating device, 80-three-dimensional localization knob.
Fig. 1 is existing typical capillary pipe electrophoresis electrochemistry detecting pool synoptic diagram
Fig. 2 is self-align capillary pipe electrophoresis electrochemistry detecting pool synoptic diagram.
Fig. 3 is self-align capillary pipe electrophoresis electrochemistry detecting pool side diagrammatic sketch.
Fig. 4 is self-align capillary pipe electrophoresis electrochemistry detecting pool top cover synoptic diagram.
Fig. 5 is a pre-determined bit capillary electrophoresis electrochemical detecting electrode synoptic diagram.
Shown in Fig. 2,3, be self-align capillary pipe electrophoresis electrochemistry detecting pool, the employing transparent insulation material is made, and includes pre-determined bit capillary electrophoresis electrochemical detecting electrode 1, ground-electrode 3 ', auxiliary electrode 3 "; 4, two electrode terminals 5 of insulated conductor, fixed block 6; kapillary 9, electrolytic cell are used for fixing garden disc electrode 3 and hold electrolytic solution 2.Pre-determined bit capillary electrophoresis electrochemical detecting electrode 1, be a slice square or rectangle transparent insulation material thin slice, be of a size of: 2cm * 2cm * 0.5cm, the electrolytic solution via 7 that a rectangular opening is 1cm * 0.5cm is left at the middle part, on rectangular opening dielectric sheet material up and down, get out the circular hole of two internal diameter 400-500 μ m then by the machining of precision with drill bit, because the quartzy fusion external diameter capillaceous that Capillary Electrophoresis is used is 360 μ m, wherein wearing hole capillaceous internal diameter requirement is 400 μ m, this hole is that kapillary bullport 19, two holes become line with one heart.One of them penetrates the electrode 3 that is connected with the contact conductor that has insulation course, electrode material is the carbon-point of diameter 300-500 μ m, glue sealing with the chloroformic solution of polymethylmethacrylate makes electrode fix and make electrode and electrolytic solution 2 to insulate, wherein electrode passes electrode hole 1-1.5mm, insulate with glue on every side, radially electrode is worn into discoidly with file, be used for electrochemical detection electrode disk 3 surface and align with capillary outlet.As shown in Figure 4, detection cell top capping is provided with patchhole 19 capillaceous, inserts the contrast electrode hole 14 of contrast electrode, at least more than one lead patchhole 18, inserts the ground-electrode hole 13 of ground-electrode.Be pre-determined bit capillary electrophoresis electrochemical detecting electrode 1 as shown in Figure 5, wherein electrode suppor is that middle hollow out is used for conducting electrolytic solution and is convenient to treatment of electrode surfaces and the relative position of observation electrode surface and capillary outlet, and get out two circular holes, one of them penetrates the electrode of the contact conductor that is connected with the tape insulation layer, the glue sealing makes electrode fix and make electrode and electrolytic solution insulation, sticking lastblock rubber bumper was made fixed block 6 after guiding groove was left in the other end, was used to regulate and the degree of closeness of fixed capillary outlet with disk detecting electrode surface.Kapillary 9 bullports 19 of pre-determined bit capillary electrophoresis electrochemical detecting electrode 1 are aimed at electrode 3, make kapillary 9 outlets and electrode 3 positioned in alignment.Electrode suppor is for inserting the mobile that also therefrom takes out in the detection cell.
Claims (4)
1, a kind of self-align capillary pipe electrophoresis electrochemistry detecting pool includes electrolytic cell, electrolytic solution, auxiliary electrode, ground-electrode, contrast electrode, connector, it is characterized in that: pre-determined bit capillary electrophoresis electrochemical detecting electrode and electrode suppor.
2, self-align capillary pipe electrophoresis electrochemistry detecting pool according to claim 1 is characterized in that kapillary bullport in the described pre-determined bit capillary electrophoresis electrochemical detecting electrode is aimed at electrode hole to make capillary outlet and electrode positioned in alignment.
3, self-align capillary pipe electrophoresis electrochemistry detecting pool according to claim 1 and 2 is characterized in that described pre-determined bit capillary electrophoresis electrochemical detecting electrode is that the fixed block made by the insulating elastomer that is bonded on the detecting electrode support is regulated and the degree of closeness on fixed capillary outlet and disk detecting electrode surface.
4, self-align capillary pipe electrophoresis electrochemistry detecting pool according to claim 1 is characterized in that the mobile of described electrode suppor for inserting or take out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00218805 CN2447788Y (en) | 2000-08-11 | 2000-08-11 | Self-setting capillary electrophoretic electrochemical detecting tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00218805 CN2447788Y (en) | 2000-08-11 | 2000-08-11 | Self-setting capillary electrophoretic electrochemical detecting tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2447788Y true CN2447788Y (en) | 2001-09-12 |
Family
ID=33584112
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00218805 Expired - Fee Related CN2447788Y (en) | 2000-08-11 | 2000-08-11 | Self-setting capillary electrophoretic electrochemical detecting tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2447788Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104280434A (en) * | 2014-09-09 | 2015-01-14 | 安徽科技学院 | Coaxial positioning capillary electrophoresis-electrochemical integration detection device |
| CN106053580A (en) * | 2016-07-29 | 2016-10-26 | 颜海 | Method for splitting racemic ranolazine by high-performance capillary electrophoresis |
| CN108362756A (en) * | 2018-01-05 | 2018-08-03 | 复旦大学 | Self-positioning capillary electrophoretic ampere detecting tank of a kind of magnetic force assembling and preparation method thereof |
-
2000
- 2000-08-11 CN CN 00218805 patent/CN2447788Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104280434A (en) * | 2014-09-09 | 2015-01-14 | 安徽科技学院 | Coaxial positioning capillary electrophoresis-electrochemical integration detection device |
| CN104280434B (en) * | 2014-09-09 | 2017-08-01 | 安徽科技学院 | A kind of coaxial positioning capillary electrophoresis electrochemical integrated detecting device |
| CN106053580A (en) * | 2016-07-29 | 2016-10-26 | 颜海 | Method for splitting racemic ranolazine by high-performance capillary electrophoresis |
| CN108362756A (en) * | 2018-01-05 | 2018-08-03 | 复旦大学 | Self-positioning capillary electrophoretic ampere detecting tank of a kind of magnetic force assembling and preparation method thereof |
| CN108362756B (en) * | 2018-01-05 | 2020-07-28 | 复旦大学 | Magnetic assembly self-positioning capillary electrophoresis ampere detection tank and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101609063B (en) | Microelectrode array chip sensor for electrochemical immunological detection | |
| Tanyanyiwa et al. | Conductimetric and potentiometric detection in conventional and microchip capillary electrophoresis | |
| JP2001527637A (en) | Electrochemical detector integrated on microfabricated capillary electrophoresis chip | |
| Hu et al. | Amperometric detection in capillary electrophoresis with an etched joint | |
| KR20070026634A (en) | Connector configuration for electrochemical cells and meters for use in combination therewith | |
| CN102749322A (en) | Bipolar electrode electrochemiluminescent detection method for microfluidic droplet array | |
| CN100417937C (en) | Chip type capacitance coupling contactless conductivity detector | |
| JP2937064B2 (en) | Capillary electrophoresis chip | |
| US5169510A (en) | Ion-permeable polymer joint for use in capillary electrophoresis | |
| US10514353B2 (en) | Arrangement and method for the electrochemical analysis of liquid samples by means of lateral flow assays | |
| US6585937B1 (en) | Electrochemical analysis system | |
| EP0060082B1 (en) | Electrochemical flow cell for liquid chromatography | |
| CN2447788Y (en) | Self-setting capillary electrophoretic electrochemical detecting tank | |
| CN102323453A (en) | Fixture for conductivity test and assembling method thereof | |
| CN100429511C (en) | Electrochemical detection method and device of integrated in chip capillary electrophoresis | |
| CN2541842Y (en) | Capillary electrophoretic electrochmical detection chip | |
| CN1769884A (en) | Capillary electrophoresis chip integrated with electrochemical detection electrode made by hot pressing method | |
| You et al. | Applications of microelectrodes in capillary electrophoresis/electrochemical detection | |
| CN100557435C (en) | Direct current conductance detecting method on capillary electrophoretic column | |
| Zhang et al. | On-column amperometric detection of ofloxacin and pasiniazid in urine by capillary electrophoresis with an improved fractured joint and small detection cell | |
| CN106574909B (en) | Contact structure body and electrical measurement device for biological sample using the same | |
| Jin et al. | A new capillary electrophoresis end‐column amperometric detection system without the need for capillary/electrode alignment | |
| CN211318296U (en) | Electrochemical microelectrode performance evaluation jig matched with USB interface | |
| EP0121936A2 (en) | Connection terminal assembly employable for ionic activity detector | |
| CN113325054B (en) | Fully-integrated portable carbon fiber microelectrode electrochemical sensor and detection system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |