CN2438121Y - Biological chip for discriminating and diagnosing virus hepatitis - Google Patents
Biological chip for discriminating and diagnosing virus hepatitis Download PDFInfo
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- CN2438121Y CN2438121Y CN 00226807 CN00226807U CN2438121Y CN 2438121 Y CN2438121 Y CN 2438121Y CN 00226807 CN00226807 CN 00226807 CN 00226807 U CN00226807 U CN 00226807U CN 2438121 Y CN2438121 Y CN 2438121Y
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Abstract
The utility model relates to a discriminating and diagnosing biological chip for hepatitis viruses of A, B, C, D, E and F types, which is composed of a specific IgG antibody detecting device, a specific IgM antibody detecting device and an HBs antigen detecting device, wherein each of the detecting device is composed of a plastic plate, a glass fiber sample-adding region arranged on the plastic plate, a film observing region and a piece of absorbent paper; the glass fiber sample-adding region and the film observing region are respectively provided with antigens or antibodies, and a gold standard technology is used for discriminating the types of the hepatitis viruses. The utility model has convenient operation, and can simultaneously detect a plurality of pathogens, and results can be instantly and accurately observed by naked eyes.
Description
The utility model relates to the antidiastole biochip of a kind of first (HAV), second (HBV), third (HCV), fourth (HDV), penta (HEV), heptan (HGV) Hepatitis virus.
All kinds of hepatitis viruss are very big to the harm of population health, can cause serious cirrhosis even liver cancer, and clinical correct diagnosis is differentiated particularly important.These pathogen IgG, IgM detection of antibodies adopt enzyme linked immunosorbent assay usually at present, it needs Medical Devices such as hydro-extractor, incubator, microplate reader, and complicated operating process, can only detect a kind of a kind of albumen of pathogen at every turn, the case that need save some when this method detects, therefore can not carry out anywhere or anytime, it is longer to detect required time.
It is a kind of easy to use, simple to operate that the utility model provides, can instant, accurate, the disposable biochip that first (HAV), second (HBV), third (HCV), fourth (HDV), penta (HEV), the multiple hepatitis virus of (HGV) type in heptan are carried out antidiastole.
For achieving the above object, the technical solution adopted in the utility model is:
A kind of biochip of virus hepatitis antidiastole, its special character is: described chip is made of at least one specific IgG antibodies pick-up unit 21, at least one specific IgM antibodies pick-up unit 22 and a HBs Detection of antigen device 23; Pick- up unit 21,22,23 is arranged at the glass fibre sample application zone 1 on the plastic plate 11 by plastic plate 11, and the film adjacent with sample application zone 1 observes district 9 and thieving paper 10 constitutes;
(a) containing gold after the affinity chromatography on the sample application zone 1 of a described IgG antibody test device 21, to mark anti-human IgG two anti-, establish the specific IgG antibodies detection line 2,4,5,6,7 that contains first (HAV), third (HCV), fourth (HDV), penta (HEV), the antigen in heptan (HGV) on the film in observation district 9, with the specific IgG antibodies detection line 3 that contains HBc antigen, observe the control line 8 that contains human IgG is set in the film of distinguishing after 9;
(b) containing gold after the affinity chromatography on the sample application zone 1 of a described IgM antibody test device 22, to mark anti-people IgM two anti-, establish the specific IgM antibodies detection line 15,17,18,19,20 that contains first (HAV), third (HCV), fourth (HDV), penta (HEV), the antigen in heptan (HGV) on the film in observation district 9, with the specific IgM antibodies detection line 16 that contains HBc antigen, observe the control line 12 that contains people IgM is set in the film of distinguishing after 9;
(c) contain the antibody that gold is marked anti-HBs on the sample application zone 1 of a described HBs Detection of antigen device 23, the film of observing district 9 is provided with the HBs Detection of antigen line 13 that contains anti-HBs antibody, and interior setting of the film behind the observation district 9 contained two anti-HBs Detection of antigen control lines 14.
Above-mentioned IgG antibody test device and IgM antibody test device can be two, three, four, five, six, the film that pick-up unit is observed the district contain first (HAV), second (HBV), third (HCV), fourth (HDV), penta (HEV), heptan (HGV) but the specific antibody detection line combination in any of six kinds of antigens;
The utility model also can adopt following technical scheme:
A kind of virus hepatitis antidiastole biochip, its special character is: described chip is by six specific IgG antibodies pick-up units in first (HAV), second (HBV), third (HCV), fourth (HDV), penta (HEV), heptan (HGV), and six specific IgM antibodies pick-up units and HBs Detection of antigen device constitute; Above-mentioned detection device is by plastic plate 11, and glass fibre sample application zone 1 thereon is set, and the film adjacent with sample application zone 1 observes district 9 and thieving paper 10 constitutes.
(a) sample application zone 1 glass fibre of described individual event IgG antibody test device contains the gold mark antigen of the corresponding specific antigen of a kind of pathogen, observe district's 9 films and contain the anti-IgG antibody detection line 24 of anti-IgG two, contain the IgG antibody test control line 25 of corresponding antibody of the specific antigen of corresponding virus;
(b) sample application zone 1 glass fibre of described individual event IgM antibody test device contains the gold mark antigen of the corresponding specific antigen of a kind of pathogen, observes the IgM antibody test control line 27 of corresponding antibody that district's 9 films contain the anti-IgM antibody detection line 26 of anti-IgM two and contain the specific antigen of corresponding virus;
(c) contain the anti-HBs antibody of gold mark on the sample application zone 1 of described HBs Detection of antigen device, the film of observing district 9 is provided with and contains anti-HBs antibody HBs Detection of antigen line 13, is provided with in the film behind the observation district 9 and contains two anti-HBs Detection of antigen control lines 14.
The antibody test device of above-mentioned two kinds of IgG, IgM with a kind of virus can be placed on same the antibody test device, sample application zone 1 glass fibre of antibody test device contains the gold mark antigen of the corresponding specific antigen of a kind of pathogen, observe district's 9 films and contain the anti-IgG antibody detection line 24 of anti-IgG two and contain the anti-IgM antibody detection line 26 of anti-IgM two, film contains the IgG and the IgM antibody test control line 28 of the corresponding antibody of corresponding specific antigen.
The film in the observation district 9 of above-mentioned two technical schemes is nitrocellulose filter or nitric acid, cellulose acetate hybrid films.
The utility model compared with prior art, adopt gold mark albumen technology, so it is easy to use, simple to operate, without any need for equipment, with the naked eye can be immediately, observations accurately, can adopt different samples such as whole blood, blood plasma, serum to diagnose, detection speed is fast, the disposable antidiastole of finishing all hepatitis viruss, thereby have very high Clinical differential diagnosis value.
Below in conjunction with the drawings and specific embodiments the utility model is described in further detail
Fig. 1 is the specific IgG antibodies pick-up unit cut-away view of the hepatitis virus of first kind of embodiment of the present utility model;
Fig. 2 is the specific IgM antibodies pick-up unit cut-away view of the hepatitis virus of first kind of embodiment of the present utility model;
Fig. 3 is the specificity HBs Detection of antigen device cut-away view of the HBV hepatitis virus of first kind of embodiment of the present utility model;
Fig. 4 is the specific IgG antibodies pick-up unit cut-away view of first (HAV) Hepatitis virus of second kind of embodiment of the present utility model;
Fig. 5 is the specific IgM antibodies pick-up unit cut-away view of first (HAV) Hepatitis virus of second kind of embodiment of the present utility model;
Fig. 6 is specific IgG, the IgM antibody test device cut-away view of first (HAV) Hepatitis virus of the third embodiment of the present utility model;
Referring to accompanying drawing, its label declaration is as follows:
The 1-sample application zone, 2-first (HAV) IgG antibody detection line, 3-second (HBV) IgG antibody detection line, 4-third (HCV) IgG antibody detection line, 5-fourth (HDV) IgG antibody detection line, 6-penta (HEV) IgG antibody detection line, 7-heptan (HGV) IgG antibody detection line, 8-contains the IgG antibody test control line of IgG, 9-observes the district, 10-level water paper, the 11-plastic plate, 12-contains the IgM antibody test control line of IgM, 13-contains the HBs Detection of antigen line of anti-HBs antibody, 14-contains two anti-HBs Detection of antigen control lines, 15-first (HAV) IgM antibody detection line, 16-second (HBV) IgM antibody detection line, 17-third (HCV) IgM antibody detection line, 18-fourth (HDV) IgM antibody detection line, 19-penta (HEV) IgM antibody detection line, 20-heptan (HGV) IgM antibody detection line, 21-IgG antibody test device, 22--IgM antibody test device, 23-HBs Detection of antigen device, 24-contains the IgG antibody detection line that anti-IgG two resists, 25-contains the IgG antibody test control line of the corresponding antibody of first (HAV) specific antigen, and 26-contains the IgM antibody detection line that anti-IgM two resists, and 27-contains the IgM antibody test control line of the corresponding antibody of first (HAV) specific antigen, 28-contains the IgG and the IgM antibody test control line of the corresponding antibody of first (HAV) specific antigen
Referring to Fig. 1, Fig. 2, Fig. 3, first kind of embodiment of the present utility model is as follows, left end on plastic plate 11 is provided with sample application zone 1 its right-hand member thieving paper 10 is set, and is middle for having the observation district 9 of film, the diaphragm employing nitrocellulose filter in observation district 9 or nitric acid, cellulose acetate hybrid films.Add sample in sample application zone 1, sample can adopt whole blood, serum or secretion, and its sample is marked two with the gold in the sample application zone 1 and resisted mixing mutually, and distinguishes 9 along the left end of device to right-hand member chromatography to observation.In tested sample, contain above-mentioned first (HAV), second (the anti-HBc antibody of HBV), third (HCV), fourth (HDV), penta (HEV), heptan (HGV) six kinds of viruses specific IgG or during IgM antibody, can mark anti-IgG or IgM antibodies with the gold in the sample application zone 1, in conjunction with compound will install chromatography to observing in the district 9 along this, with respectively with observe district 9 in six kinds of IgG and IgM antibody detection line on wrap quilt corresponding specific antigen combine, in observing the district, form the red stripes that naked eyes can be debated, excessive compound or unconjugated gold mark thing can continue chromatography, the formation red stripes that combines with IgG on control line 8 or 12 or IgM, but visual inspection result; If 2,3,4,5,6,7 as red stripes occurring on the IgG antibody test device, then table shines the IgG antibody positive of above-mentioned 6 kinds of viruses, otherwise negative; IgM antibody test device 15,16,17,18,19,20 then shows the IgM antibody positive of above-mentioned 6 kinds of pathogen as red stripes occurring, otherwise negative; HBs Detection of antigen device 13 positions then show the HBs Detection of antigen positive as red stripes occurring, otherwise negative.The result appearred in the strong positive sample at 5～10 minutes, generally got final product observations at 25～30 minutes, and control line is nature controlling line also, and when detecting, control line all should form red stripes, otherwise this pick-up unit lost efficacy.
Hepatitis antidiastole chip among the embodiment 1 can be divided into IgG antibody test and two kinds of detection chip of IgM antibody test, uses respectively; It also should belong to protection domain of the present utility model.
Above-mentioned IgG antibody test device and IgM antibody test device can be two, three, four, five, six, the film that pick-up unit is observed the district contain first (HAV), second (HBV), third (HCV), fourth (HDV), penta (HEV), heptan (HGV) but the specific antibody detection line combination in any of six kinds of antigens.
Above-mentioned hepatitis antidiastole chip also can be made of a new IgG or IgM antidiastole chip any two kinds, three kinds, four kinds or five kinds in six kinds of pathogen.Detect its IgG and/or IgM antibody.The for example combination of third (HCV) and fourth (HDV) type hepatitis.
Referring to Fig. 4, Fig. 5, embodiment for second kind of technical scheme of the utility model: this mode will detect above-mentioned first (HAV), second (the anti-HBc antibody of HBV), third (HCV), fourth (HDV), penta (HEV), heptan (HGV), 6 kinds of virus IgG antibodies detected respectively, Fig. 4 is the specific IgG antibodies pick-up unit cut-away view of first (HAV) Hepatitis virus, contain gold mark antigen in the sample application zone 1 respectively, observe the detection of antibodies control line 25 that district 9 is provided with the specific antigen of the corresponding pathogen that includes the anti-specific IgG antibodies detection line 24 of anti-human IgG two and include first (HAV), its principle is identical with IgG antibody test principle among the embodiment 1; Above-mentioned first (HAV), second (the anti-HBc antibody of HBV), third (HCV), fourth (HDV), penta (HEV), heptan (HGV), 6 kinds of virus IgM antibodies also can detect respectively, Fig. 5 is the specific IgM antibodies pick-up unit cut-away view of first (HAV) Hepatitis virus, contain gold mark antigen in the sample application zone 1 respectively, observe the detection of antibodies control line 27 that district 9 is provided with the specific antigen of the corresponding pathogen that includes the anti-specific IgM antibodies detection line 26 of anti-people IgM two and include first (HAV), its principle is identical with IgM antibody test principle among the embodiment 1.
Referring to Fig. 6, be the another kind of embodiment of second kind of technical scheme: this mode with the IgG of each pathogen and IgM detect at same device, Fig. 6 is specific IgG, the IgM antibody test device cut-away view of first (HAV) Hepatitis virus, contain gold mark antigen in the sample application zone 1 respectively, observe IgG and the IgM antibody test control line 28 establishing the anti-specific IgG detection line 24 of the anti-human IgG of gold mark two in the district 9 and be provided with the antibody of the corresponding pathogen specific antigen that contains the anti-specific IgM detection line 26 of anti-people IgM two and contain first (HAV), detect principle with embodiment 1.
Claims (5)
1, a kind of virus hepatitis antidiastole biochip is characterized in that: described chip is made of at least one specific IgG antibodies pick-up unit (21), at least one specific IgM antibodies pick-up unit (22) and a HBs Detection of antigen device (23); Pick-up unit (21,22,23) is arranged at the glass fibre sample application zone (1) on the plastic plate (11) by plastic plate (11), and the film adjacent with sample application zone (1) observes district (9) and thieving paper (10) constitutes;
(a) containing gold after the affinity chromatography on the sample application zone (1) of a described IgG antibody test device (21), to mark anti-human IgG two anti-, observe on the film of distinguishing (9) and establish the specific IgG antibodies detection line (2,4,5,6,7) that contains first (HAV), third (HCV), fourth (HDV), penta (HEV), the antigen in heptan (HGV), with the specific IgG antibodies detection line (3) that contains HBc antigen, observe the interior control line (8) that contains human IgG that is provided with of film in district (9);
(b) containing gold after the affinity chromatography on the sample application zone (1) of a described IgM antibody test device (22), to mark anti-people IgM two anti-, observe on the film of distinguishing (9) and establish the specific IgM antibodies detection line (15,17,18,19,20) that contains first (HAV), third (HCV), fourth (HDV), penta (HEV), the antigen in heptan (HGV), with the specific IgM antibodies detection line (16) that contains HBc antigen, the control line (12) that contains people IgM is set in the film behind the observation district (9);
(c) contain the antibody that gold is marked anti-HBs on the sample application zone 1 of a described HBs Detection of antigen device (23), the film of observing district (9) is provided with the HBs Detection of antigen line (13) that contains anti-HBs antibody, is provided with in the film behind the observation district (9) to contain two anti-HBs Detection of antigen control lines (14).
2, virus hepatitis antidiastole biochip according to claim 1, it is characterized in that: described IgG antibody test device and IgM antibody test device can be divided into two, three, four, five, six, the film that pick-up unit is observed the district contain first (HAV), second (HBV), third (HCV), fourth (HDV), penta (HEV), heptan (HGV) but the specific antibody detection line combination in any of six kinds of antigens.
3, a kind of virus hepatitis antidiastole biochip, it is characterized in that: described chip is by six specific IgG antibodies pick-up units in first (HAV), second (HBV), third (HCV), fourth (HDV), penta (HEV), heptan (HGV), and six specific IgM antibodies pick-up units and HBs Detection of antigen device constitute; Above-mentioned detection device is provided with glass fibre sample application zone (1) thereon by plastic plate (11), and the film adjacent with sample application zone (1) observes district (9) and thieving paper (10) constitutes;
(a) sample application zone (1) glass fibre of described individual event IgG antibody test device contains the gold mark antigen of the corresponding specific antigen of a kind of pathogen, observe the IgG antibody test control line (25) of corresponding antibody that district's (9) film contains the anti-IgG antibody detection line (24) of anti-IgG two and contains the specific antigen of corresponding virus;
(b) sample application zone (1) glass fibre of described individual event IgM antibody test device contains the gold mark antigen of the corresponding specific antigen of a kind of pathogen, observe the IgM antibody test control line (27) of corresponding antibody that district's (9) film contains the anti-IgM antibody detection line (26) of anti-IgM two and contains the specific antigen of corresponding virus;
(c) contain the anti-HBs antibody of gold mark on the sample application zone (1) of described HBs Detection of antigen device, the film of observing district (9) is provided with the antibody HBs Detection of antigen line (13) that contains anti-HBs antigen, and back, observation district (9) is provided with contains two anti-HBs Detection of antigen control lines (14).
4, virus hepatitis antidiastole biochip according to claim 3, it is characterized in that: described two kinds of IgG with a kind of virus, the antibody test device of IgM can be placed on same the antibody test device, sample application zone 1 glass fibre of antibody test device contains the gold mark antigen of the corresponding specific antigen of a kind of pathogen, observe film behind the district (9) and contain the anti-IgG antibody detection line (24) of anti-IgG two and contain the anti-IgM antibody detection line (26) of anti-IgM two, control line contains the IgG and the IgM antibody test control line (28) of corresponding specific antigen correspondence antibody.
5, require any described virus hepatitis antidiastole biochip according to aforesaid right, it is characterized in that: the film of observing district (9) is nitrocellulose filter or nitric acid, cellulose acetate hybrid films.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00226807 CN2438121Y (en) | 2000-09-18 | 2000-09-18 | Biological chip for discriminating and diagnosing virus hepatitis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00226807 CN2438121Y (en) | 2000-09-18 | 2000-09-18 | Biological chip for discriminating and diagnosing virus hepatitis |
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| Publication Number | Publication Date |
|---|---|
| CN2438121Y true CN2438121Y (en) | 2001-07-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00226807 Expired - Fee Related CN2438121Y (en) | 2000-09-18 | 2000-09-18 | Biological chip for discriminating and diagnosing virus hepatitis |
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| CN (1) | CN2438121Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002077152A1 (en) * | 2001-03-28 | 2002-10-03 | Genetech Biotechnology (Shanghai) Company Limited | Device and method for detection of multiple analytes |
| CN101553732B (en) * | 2006-08-31 | 2013-12-25 | 大阪府 | Rapid diagnosis method specific to avian influenza virus |
| CN104730244A (en) * | 2014-10-24 | 2015-06-24 | 浙江东方基因生物制品有限公司 | Immunochromatography test paper for detecting human HEV IgM and IgG and preparation method of immunochromatography test paper |
| CN107478836A (en) * | 2017-06-29 | 2017-12-15 | 哈尔滨医科大学 | Kit that is a kind of while detecting hepatitis A, hepatitis B and hepatitis and its production and use |
-
2000
- 2000-09-18 CN CN 00226807 patent/CN2438121Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002077152A1 (en) * | 2001-03-28 | 2002-10-03 | Genetech Biotechnology (Shanghai) Company Limited | Device and method for detection of multiple analytes |
| CN101553732B (en) * | 2006-08-31 | 2013-12-25 | 大阪府 | Rapid diagnosis method specific to avian influenza virus |
| CN104730244A (en) * | 2014-10-24 | 2015-06-24 | 浙江东方基因生物制品有限公司 | Immunochromatography test paper for detecting human HEV IgM and IgG and preparation method of immunochromatography test paper |
| CN107478836A (en) * | 2017-06-29 | 2017-12-15 | 哈尔滨医科大学 | Kit that is a kind of while detecting hepatitis A, hepatitis B and hepatitis and its production and use |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |