CN2398630Y

CN2398630Y - Capillary cataphoresis small-sized ampere detecting pond with high-tension electric field isolation interface

Info

Publication number: CN2398630Y
Application number: CN 99248969
Authority: CN
Inventors: 袁倬斌; 张书胜
Original assignee: Individual
Current assignee: Individual
Priority date: 1999-10-08
Filing date: 1999-10-08
Publication date: 2000-09-27
Anticipated expiration: 2009-10-08

Abstract

The utility model provides a novel capillary cataphoresis small-sized ampere detecting pond with a high-tension electric field isolation interface. The utility model is mainly characterized in that the utility model comprises a small-sized interface pond and a small-sized electrode pond. The utility model has the advantages of simple fabricating method, small size and convenient cleanness of the electrode pond. A separating capillary with the high-tension electric field isolation interface can be conveniently replaced, and the service life of the separating capillary can be extended; a working microelectrode can be accurately and conveniently inserted into a detecting capillary, and therefore, the utility model has convenient installation.

Description

The small-sized amperometric detection cell of Capillary Electrophoresis of band high-voltage electric field isolating interface

The utility model relates to the small-sized amperometric detection cell of a kind of Capillary Electrophoresis with the high-voltage electric field isolating interface.

Detection technique is one of core technology of Capillary Electrophoresis, and the galvanochemistry Amperometric Detection Coupled is the new technology that grows up the end of the eighties, and its detection sensitivity is far above ultraviolet detection, and it is suitable with detection technique of fluorescence, it is made simply simultaneously, and is cheap, therefore has been subjected to great attention.Amperometric Detection Coupled has been one of most active problem in the analytical chemistry subject since the nineties.1995～1998 years, obtain the subsidy of state natural sciences fund with regard to the research that multinomial capillary electrophoresis electrochemical Amperometric Detection Coupled is arranged, resemble research groups such as Ewing, Lunte, Baldwin abroad and all dropped into bigger energy research ampere detector and detection method.At present, one of problem that ampere detector exists is that its small detection electric current (nA-pA) is subjected to having a strong impact on of capillary electrophoresis separation high-voltage electric field (500-1000V/cm), and noise is bigger, uses the high-voltage electric field isolating interface to address this problem.

Amperometric detection cell is the important component part of ampere detector, because the used internal diameter capillaceous of Capillary Electrophoresis is usually between 10～50 μ m, the diameter of used work microelectrode is usually between 10～50 μ m, the placement location of work microelectrode and mode, the size of electrode cell, the shape that mode has determined Amperometric Detection Coupled, and the result of Amperometric Detection Coupled.(inscription on ancient bronze objects is farsighted for Li Guanbin, Du Bin, Wang Shuren, Ou Qingyu, Yu Weile, analytical chemistry, 1995,23 volumes, 480-484 page or leaf for traditional Amperometric Detection Coupled on the post that has; Hu Shen, Pang Daiwen, Li Peibiao, Cheng Jieke, chromatogram, 1996,14 volumes, 256-258 page or leaf; Park S, Lunte S M, Lunte C E, Analytical Chemistry, 1995,67 volumes, 911-918 page or leaf; Zhou J, Lunte C E, Lunte S M, Analytical Chemica Acta, 1999,379 volumes, the 307-317 page or leaf), it need utilize three-dimensional micro actuator in microscopically will be worked microelectrode insertion detection kapillary, and very inconvenience is installed, interface repository and electrode cell volume are big, and kapillary is difficult for changing.Another detection mode is that styletable detects (Xu Danke, Hua Lin, Chen Hongyuan, Zhu Shutao, high chemical journal, 1996,17 volumes, 707-709 page or leaf; Chen M, Huang H, Analytical Chemistry, 1995,67 volumes, 4010-4014 page or leaf; Zhu Zhu, Hu Shen, Cheng Jieke, analytical test journal, 1999, the 4th phase, the 36-38 page or leaf), it does not use high-voltage electric field isolating interface itself just to have the interference of high-voltage electric field, coulombic efficiency is lower, shortcomings such as detection sensitivity is restricted, though saved interface repository, its designed electrode cell volume is still bigger.As fully visible, the ubiquitous problem of Amperometric Detection Coupled has (a) electrode cell and interface repository volume big, not easy cleaning; (b) joint strength that has now used is low, the weak effect of isolated high-voltage electric field, and the life-span is short; (c) kapillary directly is fixed on electrode cell and the interface repository, is difficult for changing; (d) the work microelectrode is installed inconvenient.

The purpose of this utility model provides a kind of small-sized amperometric detection cell of Capillary Electrophoresis of new band high-voltage electric field isolating interface, its method for making is simple, volume is little, the work microelectrode can insert easily and detect in the kapillary, the separation capillary of band high-voltage electric field isolating interface can be changed easily, prolonged the life-span, the cleaning of electrode cell is also very convenient.

The technology implementation scheme of the small-sized amperometric detection cell of Capillary Electrophoresis of band high-voltage electric field isolating interface of the present utility model is illustrated with Fig. 1 (sectional view) and Fig. 2 (vertical view), wherein: the 1-separation capillary, 2,12,13, the 17-screw, the negative wire Pt silk of 3-capillary electrophoresis separation high pressure, 4-high-voltage electric field isolating interface (is called for short interface, this interface has used our another utility model patent " the solid high-voltage electric field isolating interface of single wall taper glue "), 5-detects kapillary, the contrast electrode Ag silk of 6-ampere detector, the auxiliary electrode Pt silk of 7-ampere detector, the 8-microelectrode of working, the lead of 9-work microelectrode, 10-interface repository, the 11-electrode cell, 14-interface repository pond body, 15-electrode cell pond body, 16-electrode capillary.

One of technological improvement of the present utility model: interface repository 10 and electrode cell 11 volumes are little, are shaped as right cylinder, have reduced the volume of ampere detector, and electrode cell 11 is convenient to clean, and has guaranteed the repeatability of testing result, has improved detection sensitivity.

As shown in Figure 1, 2, the material of the

pond body

14,15 of small-sized amperometric detection cell is organic glass, and its full-size is 1.8cm * 1.8cm * 1.5cm (length * wide * height).Interface repository 10 and electrode cell 11 be shaped as right cylinder, maximum gauge is 8mm, height is 10mm.

Two of technological improvement of the present utility model: use the separation capillary 1 of screw 2,17 fixed band interfaces 4, make it easy for installation, change easily, increased serviceable life.

As shown in Figure 1, 2, get the internal thread that two diameters are φ 6mm to the maximum respectively in interface repository pond body 14 obverse centers and electrode cell pond body 15 obverse centers, it is fixing that interface 4 two ends are passed screw 2, interface repository 10 and screw 17 backs respectively, the center of

screw

2,12,13,17 is hollow, and the external diameter of its diameter and

kapillary

1,5,16 is complementary.Opening a φ on interface repository pond body 14 is 0.3～0.6mm aperture, communicates with interface repository 10, and the negative wire Pt silk 3 of capillary electrophoresis separation high pressure can be drawn by aperture.

Three of technological improvement of the present utility model: the microelectrode 8 of will working is fixed in the electrode capillary 16, and electrode capillary 16 is fixed on the screw 13, work microelectrode 8 can be inserted accurately and conveniently detect in the kapillary 5, and it is very convenient to install.

As shown in Figure 1, 2, an end that detects kapillary 5 passes screw 12 backs and fixes, the electrode capillary 16 that is used for fixing work microelectrode 8 and work microelectrode lead 9 passes screw 13 backs and fixes, opening two φ on electrode cell pond body 15 is 0.3～0.6mm aperture, communicate with electrode cell 11, the contrast electrode Ag silk 6 of ampere detector and auxiliary electrode Pt silk 7 can be drawn by two apertures respectively.

Four of technological improvement of the present utility model: after having used our another utility model patent " the solid high-voltage electric field isolating interface of single wall taper glue ", isolated the interference of high-voltage electric field, reduced noise, improved detection sensitivity to detection signal.

In a word, the small-sized amperometric detection cell of Capillary Electrophoresis of band high-voltage electric field isolating interface provided by the utility model, its method for making is simple, volume is little, the cleaning of electrode cell is convenient, and separation capillary can be changed easily, prolongs the serviceable life of separation capillary, the work microelectrode can insert accurately and conveniently and detect in the kapillary simple installation.

Claims

1, the small-sized amperometric detection cell of a kind of Capillary Electrophoresis with the high-voltage electric field isolating interface, it is characterized in that forming, and by screw 2,17 and 12 interface repository 10 and electrode cell 11 are linked together by separation capillary 1 and detection kapillary 5 by interface repository 10 and electrode cell 11.

2, the small-sized amperometric detection cell of Capillary Electrophoresis of band high-voltage electric field isolating interface according to claim 1 is characterized in that interface repository pond body 14 and electrode cell pond body 15 made by organic glass, and being shaped as of interface repository 10 and electrode cell 11 is cylindrical.

3, the small-sized amperometric detection cell of Capillary Electrophoresis of band high-voltage electric field isolating interface according to claim 1 is characterized in that work microelectrode 8 is fixed in the electrode capillary 16, and electrode capillary 16 is fixed on the screw 13.

4, the small-sized amperometric detection cell of Capillary Electrophoresis of band high-voltage electric field isolating interface according to claim 1 is characterized in that separation capillary 1 and detection kapillary 5 successively pass screw 2,17 and 12, are separately fixed on interface repository 10 and the electrode cell 11.