CN2213997Y - Checkout apparatus for spot immunity with one-step percolation method - Google Patents
Checkout apparatus for spot immunity with one-step percolation method Download PDFInfo
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- CN2213997Y CN2213997Y CN 94220268 CN94220268U CN2213997Y CN 2213997 Y CN2213997 Y CN 2213997Y CN 94220268 CN94220268 CN 94220268 CN 94220268 U CN94220268 U CN 94220268U CN 2213997 Y CN2213997 Y CN 2213997Y
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- bowl
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Abstract
The utility model relates to a detection device for spot immunity with a one-step microporous membrane percolation method. A colloidal gold marking object combined with an object to be tested is dried previously in a specimen bowl (the bowl is clamped in a specimen hole of a percolation box). After dropped into the specimen bowl, the object to be tested flows to the bottom of the bowl along a fibrous pad and the bowl wall. Therefore, the object to be tested can be combined with the gold marking object. The object to be tested then passes through a small hole at the bottom of the bowl and the spotted closed microporous membrane in the percolation box of filter paper at the outer bottom, so that the object to be tested and the gold marking object can be combined in a spotted position so as to display spots. Excess liquid is absorbed by an absorption pad below the microporous membrane.
Description
The utility model relates to a kind of micro substance immunoassay device, especially a step percolation spot immune pick-up unit.
At present, for commonly used, this method needs additional solutions reagent to known diafiltration spot immune detection technique with the micropore embrane method, and must the operation of two, three steps just can finish.
The purpose of this utility model provides an a kind of step percolation spot immune detecting device, and it is predrying in device with solution reagent, can a step add determinand and finish detection, and prolong reagent serviceable life greatly.
The purpose of this utility model is achieved in that collaurum target antibody (or other labels) is splashed in the bowl shaped container (sample bowl), flood a bowl interior fiber mat, after treating bowl interior solution drying (as freeze-drying), open an aperture at bowl bottom, outer bottom pastes a filter paper (can contain the hydrotrope).Above-mentioned sample bowl and content thereof and filter paper are placed on the specimen hole of existing spot immune percolation box, and this box has been equipped with point sample (having adsorbed the material that can combine with determinand) and has sealed the microporous barrier (as nitrocellulose filter) and the adsorptive pads of all the other binding sites.There are fiber mat and filter paper to invest bowl wall and the bowl end in the sample bowl, specimen fluids flows into the bowl end along fiber mat, a bowl interior freeze-dried reagent is dissolved very soon, determinand combines with label rapidly in the sample, flow in the percolation box specimen hole by bowl end aperture and outer bottom filter paper then, permeate microporous barrier again, determinand and label bond can be combined with the point sample thing, label just can show visible spot at the point sample place like this.Adsorptive pads below the unnecessary liquid infiltration into microporous film.After treating that liquid has all oozed, remove sample bowl and filter paper so that observations.If do not contain determinand in the sample, label just can not stay in the point sample place, also display dot not just.Can detect determinand special, sensitively thus.
Owing to adopt such scheme, can a step finish microporous barrier percolation spot immune and detect, and the reagent life-span prolong.
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1 is that embodiment overlooks structural map.
Fig. 2 is that embodiment hits exactly the vertical section structural map.
Prominent 5. microporous barriers in 2. bowls of covers of 1. sample bowls and handle 3. fiber mats and interior filter paper 4. specimen holes of bowl and edge 6. percolation boxs 7. apertures 8. outer bottom filter paper 9. are inhaled pad among the figure
In the accompanying drawings, sample bowl (1) with in soft material make so that spile (7) at the bottom of bowl, fiber mat (3) is in the sample bowl.Bowl cover (2) is the annular ring around bowl (1) lateral wall, the edge of sample bowl (1) and outer bottom filter paper (8) can be tightened therebetween; Bowl cover (2) periphery also has ring groove, and this groove is clamped in specimen hole along prominent (4), bowl cover (2) is fixed in the specimen hole (4) of percolation box (6).Pull bowl cover handle (2) and sample bowl (1) and bowl cover (2) can be removed exposure microporous barrier (5).(central authorities can open for specimen hole (4) percolation box (6) loam cake, are used for microporous barrier (5) being clamped in specimen hole (4) and inhaling between the pad (9).During the drying label, label is dripped in sample bowl (1), and flood fiber mat (3), spile at the bottom of bowl after the label drying (7) add a bowl interior filter paper (3) again.When adding sample, specimen fluids is contacted with fiber mat (3) with bowl (1) perisporium (or filter paper 3), make the suitable bowl of liquid wall flow into a bowl end, inhale in the pad (9) through aperture at the bottom of the bowl (7), outer bottom filter paper (8) infiltration microporous barrier (5), infiltration again.After treating that liquid has all oozed (needing several minutes), remove sample bowl (1) and bowl cover (2) and have or not spot to form on the microporous barrier (5) to observe.
Claims (2)
1, a kind of microporous barrier diafiltration spot immune detecting device, make point sample place on determinand and the label infiltration microporous barrier, it is characterized in that: label is predrying in this device in the sample bowl (1) of circular or other shapes, aperture (7) is arranged at the bottom of this bowl, in the bowl fiber mat or filter paper are arranged, logical microporous barrier (5) of bowl outer bottom and suction pad (9).
2, according to the described detecting device of claim 1, it is characterized in that: sample bowl and content thereof are fixed in through the bowl cover on the specimen hole of percolation box, also can be used for other diafiltration spot immune pick-up units.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94220268 CN2213997Y (en) | 1994-08-30 | 1994-08-30 | Checkout apparatus for spot immunity with one-step percolation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94220268 CN2213997Y (en) | 1994-08-30 | 1994-08-30 | Checkout apparatus for spot immunity with one-step percolation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2213997Y true CN2213997Y (en) | 1995-11-29 |
Family
ID=33834912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94220268 Expired - Fee Related CN2213997Y (en) | 1994-08-30 | 1994-08-30 | Checkout apparatus for spot immunity with one-step percolation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2213997Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102053153A (en) * | 2010-11-25 | 2011-05-11 | 西安微通生物技术有限公司 | Dot immuno gold directed infiltration detection kit and application thereof |
| CN103323590A (en) * | 2013-06-08 | 2013-09-25 | 上海云泽生物科技有限公司 | Quantitative detection device based on fibrous-membrane gathering and separation and detection method thereof |
| CN106771145A (en) * | 2016-08-23 | 2017-05-31 | 广东优尼德生物科技有限公司 | One kind detection D dimers spot gold diafiltration kit and quantitative detecting method |
| CN106855573A (en) * | 2016-08-23 | 2017-06-16 | 广东优尼德生物科技有限公司 | Detection PSA spot gold diafiltration kit and its detection method |
| CN113341130A (en) * | 2021-05-31 | 2021-09-03 | 湖北美宝生物科技股份有限公司 | Early pregnancy test paper |
-
1994
- 1994-08-30 CN CN 94220268 patent/CN2213997Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102053153A (en) * | 2010-11-25 | 2011-05-11 | 西安微通生物技术有限公司 | Dot immuno gold directed infiltration detection kit and application thereof |
| CN103323590A (en) * | 2013-06-08 | 2013-09-25 | 上海云泽生物科技有限公司 | Quantitative detection device based on fibrous-membrane gathering and separation and detection method thereof |
| CN106771145A (en) * | 2016-08-23 | 2017-05-31 | 广东优尼德生物科技有限公司 | One kind detection D dimers spot gold diafiltration kit and quantitative detecting method |
| CN106855573A (en) * | 2016-08-23 | 2017-06-16 | 广东优尼德生物科技有限公司 | Detection PSA spot gold diafiltration kit and its detection method |
| CN113341130A (en) * | 2021-05-31 | 2021-09-03 | 湖北美宝生物科技股份有限公司 | Early pregnancy test paper |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |