CN221355407U - Pine wood nematode culture collection device - Google Patents
Pine wood nematode culture collection device Download PDFInfo
- Publication number
- CN221355407U CN221355407U CN202322912674.6U CN202322912674U CN221355407U CN 221355407 U CN221355407 U CN 221355407U CN 202322912674 U CN202322912674 U CN 202322912674U CN 221355407 U CN221355407 U CN 221355407U
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- China
- Prior art keywords
- dish
- fixedly connected
- dish cover
- filter screen
- screen plate
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- 241000243771 Bursaphelenchus xylophilus Species 0.000 title claims abstract description 13
- 238000007789 sealing Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 235000009754 Vitis X bourquina Nutrition 0.000 abstract description 10
- 235000012333 Vitis X labruscana Nutrition 0.000 abstract description 10
- 235000014787 Vitis vinifera Nutrition 0.000 abstract description 10
- 238000002474 experimental method Methods 0.000 abstract description 2
- 240000006365 Vitis vinifera Species 0.000 abstract 1
- 241000244206 Nematoda Species 0.000 description 11
- 241000219095 Vitis Species 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 238000012136 culture method Methods 0.000 description 3
- 241000243770 Bursaphelenchus Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of forestry experiments, in particular to a pine wood nematode culture collection device, which comprises a culture dish, wherein the culture dish comprises a dish body, a dish cover is arranged above the dish body, a sealing ring is adhered to the bottom of the inner side of the dish cover, the inner side of the sealing ring is tightly attached to the outer wall of the dish body, a water inlet is fixedly connected to the top of the right side of the dish cover, a water outlet is fixedly connected to the top of the left side of the dish cover, a sealing assembly is arranged between the dish body and the dish cover, a separation collection assembly is arranged on the inner side of the dish cover, the separation collection assembly comprises a filter screen plate arranged on the inner side of the dish cover, handles are fixedly connected to the left side and the right side of the top of the filter screen plate, connecting grooves are formed in the left side and the right side of the filter screen plate, the side wall of the filter screen plate is movably connected with the inner wall of the dish cover, ash grape hyphae are filtered through the filter screen plate, and the filter screen plate can be detached and cleaned to avoid blockage.
Description
Technical Field
The utility model relates to the technical field of forestry experiments, in particular to a pine wood nematode culture collection device.
Background
At present, in the field of forestry plant diseases and insect pests research, pine wood nematodes are very common pests, when the pine wood nematodes are researched, the most common culture method is a PDA culture medium gray grape bacteria-holding culture method, and is usually carried out in a glass culture dish, specifically, gray grape bacteria-holding culture method is carried out on a sterile solid PDA culture medium, when the culture dish is full of the gray grape bacteria-holding culture medium, the required nematodes are inoculated, after about two weeks of culture, when a large amount of gray grape bacteria-holding hyphae are seen to be eaten by the nematodes, the surface of the culture medium can be washed by sterile water to recover the nematodes; generally, a filter screen is used for filtering the ash grape hyphae, but the existing filter screen is inconvenient to detach and clean, and the filter screen is easy to block.
Disclosure of utility model
The utility model aims to provide a pine wood nematode culture collection device, which aims to solve the problems in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
The utility model provides a pine wood nematode cultivates collection device, includes the culture dish, the culture dish includes the dish body, the top of dish body is provided with the dish lid, the inboard bottom of dish lid bonds there is the sealing washer, the inboard of sealing washer is hugged closely with the outer wall of dish body, the top fixedly connected with water inlet on dish lid right side, the top fixedly connected with delivery port on dish lid left side, be provided with seal assembly between dish body and the dish lid, the inboard of dish lid is provided with separation collection assembly;
The separation collection subassembly is including setting up the filter screen plate in the dish lid inboard, the equal fixedly connected with handle in the left and right sides at filter screen plate top, the spread groove has all been seted up to the left and right sides of filter screen plate, the lateral wall and the inner wall swing joint of dish lid of filter screen plate.
As the preferable scheme of the utility model, the left side and the right side of the dish cover are fixedly connected with the supporting plates, the tops of the two supporting plates are fixedly connected with the sleeves, the inside of the two sleeves is provided with the telescopic springs, one ends of the two telescopic springs, which are opposite, are fixedly connected with the inner walls of the two sleeves, and the opposite ends of the two telescopic springs are fixedly connected with the loop bars.
As a preferable scheme of the utility model, the inner walls of the upper side and the lower side of the two sleeves are respectively provided with a limiting chute, the upper side and the lower side of the two connecting grooves close to one end of the telescopic spring are respectively connected with the inner walls of the four limiting chutes in a sliding way, and one end of the two sleeve rods, which is far away from the telescopic spring, extends to the outer parts of the two sleeves and is connected with the inner walls of the two connecting grooves in a clamping way.
As a preferable scheme of the utility model, clamping grooves are formed in the left side and the right side of the dish cover, L-shaped pull rods are movably connected to the inner walls of the two clamping grooves, and one ends, far away from the clamping grooves, of the two L-shaped pull rods are fixedly connected with the surfaces of the two loop bars.
As a preferable scheme of the utility model, the sealing component comprises fixing rods arranged at the left side and the right side of the dish cover, one opposite sides of the two fixing rods are fixedly connected with the inner walls of the two sides of the dish cover, the tops of the left side and the right side of the dish body are fixedly connected with fixing grooves, the bottom ends of the two fixing rods extend to the inside of the two fixing grooves, one sides of the two fixing grooves, far away from the dish body, are fixedly connected with fixing cylinders, locking rods penetrate through the inside of the two fixing cylinders, and one sides of the two opposite sides of the two fixing rods are provided with locking grooves.
As a preferable scheme of the utility model, one ends of the two locking rods, which are opposite, extend to the inside of the two locking grooves respectively, the surfaces of the two locking rods are fixedly connected with sliding plates, the side walls of the two sliding plates are in sliding connection with the inner walls of the two fixed cylinders, springs are sleeved on the surfaces of the two locking rods, one ends of the two springs are fixedly connected with the surfaces of the two sliding plates, and the other ends of the two springs are fixedly connected with the inner walls of the two fixed cylinders.
Compared with the prior art, the utility model has the beneficial effects that:
1. According to the utility model, the locking rod is clamped with the locking groove, so that the fixing rod and the fixing groove can be fixed, and the dish body and the dish cover are in sealing connection.
2. According to the utility model, the sleeve rod is pulled to move through the locking groove, and slides towards the inside of the sleeve barrel to squeeze the telescopic spring, so that the sleeve rod leaves the inside of the connecting groove, and the filter screen plate can be detached and cleaned.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic view of a cut-away structure of the present utility model;
FIG. 3 is an enlarged schematic view of the structure of the present utility model A;
fig. 4 is an enlarged schematic view of the structure of the present utility model at B.
In the figure: 1. a culture dish; 101. a dish body; 102. a dish cover; 103. a seal ring; 104. a water inlet; 105. a water outlet; 2. a seal assembly; 201. a fixing groove; 202. a fixed rod; 203. a fixed cylinder; 204. a locking lever; 205. a spring; 206. a sliding plate; 207. a locking groove; 3. a separation and collection assembly; 301. a filter screen plate; 302. a handle; 303. a support plate; 304. a sleeve; 305. a telescopic spring; 306. limiting sliding grooves; 307. a loop bar; 308. a connecting groove; 309. an L-shaped pull rod; 310. a clamping groove.
Detailed Description
The technical solutions of the embodiments of the present utility model will be clearly and completely described below in conjunction with the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, but not all embodiments, and all other embodiments obtained by those skilled in the art without making any inventive effort based on the embodiments of the present utility model are within the scope of protection of the present utility model.
In order that the utility model may be readily understood, several embodiments of the utility model will be described more fully hereinafter with reference to the accompanying drawings, in which embodiments of the utility model are shown, but in which the utility model may be embodied in many different forms and is not limited to the embodiments described herein, but instead is provided to provide a more thorough and complete disclosure of the utility model.
Referring to fig. 1, 2 and 4, the present utility model provides a technical solution:
The utility model provides a pine wood nematode culture collection device, including culture dish 1, culture dish 1 includes the dish body 101, the top of dish body 101 is provided with dish lid 102, the inboard bottom of dish lid 102 bonds there is sealing washer 103, the inboard of sealing washer 103 is hugged closely with the outer wall of dish body 101, the top fixedly connected with water inlet 104 on dish lid 102 right side, the top fixedly connected with delivery port 105 on dish lid 102 left side, be provided with seal assembly 2 between dish body 101 and the dish lid 102, the inboard of dish lid 102 is provided with separation collection subassembly 3, separation collection subassembly 3 is including setting up the filter screen 301 at dish lid 102 inboard, the equal fixedly connected with handle 302 in left and right sides at filter screen 301 top, spread the left and right sides of filter screen 301 has all seted up spread the spread groove 308, the lateral wall of filter screen 301 and the inner wall swing joint of dish lid 102.
As shown in fig. 4, the left and right sides of the dish cover 102 are fixedly connected with the support plates 303, the tops of the two support plates 303 are fixedly connected with the sleeves 304, the telescopic springs 305 are arranged in the two sleeves 304, one ends of the two telescopic springs 305, which are opposite, are fixedly connected with the sleeve rods 307, the inner walls of the upper side and the lower side of the two sleeves 304 are respectively provided with the limit sliding grooves 306, the upper side and the lower side of the two connecting grooves 308, which are close to one ends of the telescopic springs 305, are respectively connected with the inner walls of the four limit sliding grooves 306 in a sliding manner, one ends, which are far away from the telescopic springs 305, of the two sleeve rods 307 are connected with the inner walls of the two connecting grooves 308 in a clamping groove 310, the inner walls of the two clamping grooves 310 are respectively and movably connected with the L-shaped pull rods 309, and one ends, which are far away from the clamping grooves 310, of the two L-shaped pull rods 309 are fixedly connected with the surfaces of the two sleeve rods 307.
As shown in fig. 1 and 3, the sealing assembly 2 includes fixing rods 202 disposed on the left and right sides of the dish cover 102, opposite sides of the two fixing rods 202 are fixedly connected with inner walls of two sides of the dish cover 102, top portions of the left and right sides of the dish body 101 are fixedly connected with fixing grooves 201, bottom ends of the two fixing rods 202 extend to inner portions of the two fixing grooves 201, one sides of the two fixing grooves 201 away from the dish body 101 are fixedly connected with fixing cylinders 203, locking rods 204 penetrate through the inner portions of the two fixing cylinders 203, locking grooves 207 are formed in opposite sides of the two fixing rods 202, opposite ends of the two locking rods 204 extend to the inner portions of the two locking grooves 207 respectively, surfaces of the two locking rods 204 are fixedly connected with sliding plates 206, side walls of the two sliding plates 206 are slidably connected with inner walls of the two fixing cylinders 203, surfaces of the two locking rods 204 are sleeved with springs 205, one ends of the two springs 205 are fixedly connected with surfaces of the two sliding plates 206, and the other ends of the two springs 205 are fixedly connected with inner walls of the two fixing cylinders 203.
The working flow of the utility model is as follows: when the novel nematode culture dish is used, a small amount of nematode suspension is inoculated to the ash grape bacteria to cultivate nematodes when the ash grape bacteria grow on the bottom surface of the dish body 101, when a large amount of ash grape bacteria are taken by nematodes, a proper amount of water is added into the dish body 101 through the water inlet 104, the dish body 101 is properly shaken up and down, nematode suspension can be formed due to the water-seeking property of the nematodes, then the dish body 101 and the dish cover 102 are inverted, the nematode suspension can enter the dish cover 102 through the filter screen plate 301, ash grape bacteria and the embracing seeds can be filtered and remain in the filter screen plate 301 through the filter screen plate 301, then the pure impurity-free nematode suspension can be collected through the container, the sliding plate 206 extrudes the spring 205 through pulling the locking rod 204 to the two locking rods, the locking rod 204 leaves the interior of the locking groove 207, the dish cover 102 is separated from the dish body 101, the sleeve 307 is moved to the interior of the sleeve 304 through the L-shaped pull rod 309, the sleeve 307 extrudes the telescopic spring 305, and the other end of the sleeve 307 leaves the filter screen plate 301, and the filter screen plate 308 can be detached.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a collection device is cultivateed to pine wood nematode, includes culture dish (1), its characterized in that: the culture dish (1) comprises a dish body (101), a dish cover (102) is arranged above the dish body (101), a sealing ring (103) is adhered to the bottom of the inner side of the dish cover (102), the inner side of the sealing ring (103) is tightly attached to the outer wall of the dish body (101), a water inlet (104) is fixedly connected to the top of the right side of the dish cover (102), a water outlet (105) is fixedly connected to the top of the left side of the dish cover (102), a sealing component (2) is arranged between the dish body (101) and the dish cover (102), and a separation and collection component (3) is arranged on the inner side of the dish cover (102);
the separation collection assembly (3) comprises a filter screen plate (301) arranged on the inner side of the dish cover (102), handles (302) are fixedly connected to the left side and the right side of the top of the filter screen plate (301), connecting grooves (308) are formed in the left side and the right side of the filter screen plate (301), and the side wall of the filter screen plate (301) is movably connected with the inner wall of the dish cover (102).
2. The pine wood nematode culture collection device of claim 1, wherein: the dish cover is characterized in that the left side and the right side of the dish cover (102) are fixedly connected with supporting plates (303), the tops of the supporting plates (303) are fixedly connected with sleeves (304), telescopic springs (305) are arranged in the sleeves (304), one ends of the telescopic springs (305) opposite to each other are fixedly connected with the inner walls of the two sleeves (304), and one ends of the telescopic springs (305) opposite to each other are fixedly connected with loop bars (307).
3. The pine wood nematode culture collection device of claim 2, wherein: limiting sliding grooves (306) are formed in the inner walls of the upper side and the lower side of the sleeve (304), the upper side and the lower side of the connecting grooves (308) close to one end of the telescopic spring (305) are respectively connected with the inner walls of the four limiting sliding grooves (306) in a sliding mode, and one end, far away from the telescopic spring (305), of the sleeve rod (307) extends to the outer portions of the two sleeves (304) to be connected with the inner walls of the two connecting grooves (308) in a clamping mode.
4. The pine wood nematode culture collection device of claim 1, wherein: clamping grooves (310) are formed in the left side and the right side of the dish cover (102), L-shaped pull rods (309) are movably connected to the inner walls of the clamping grooves (310), and one ends, far away from the clamping grooves (310), of the L-shaped pull rods (309) are fixedly connected with the surfaces of the two loop bars (307).
5. The pine wood nematode culture collection device of claim 1, wherein: sealing component (2) are including setting up dead lever (202) in dish lid (102) left and right sides, two the opposite one side of dead lever (202) and the both sides inner wall fixed connection of dish lid (102), the equal fixedly connected with fixed slot (201) in top of dish body (101) left and right sides, two the bottom of dead lever (202) extends to the inside of two fixed slots (201), two the equal fixedly connected with fixed cylinder (203) in one side of dish body (101) is kept away from to fixed slot (201), two the inside of fixed cylinder (203) is run through has locking lever (204), two locking slot (207) have all been seted up to one side that dead lever (202) are on the back of the body.
6. The pine wood nematode culture collection device of claim 5, wherein: two the inside that one end that locking lever (204) is opposite extends to two locking grooves (207) respectively, two the equal fixedly connected with sliding plate (206) in surface of locking lever (204), two the lateral wall of sliding plate (206) and the inner wall sliding connection of two fixed cylinders (203), two the surface of locking lever (204) all overlaps is equipped with spring (205), two the one end and the fixed surface connection of two sliding plate (206) of spring (205), two the other end and the inner wall fixed connection of two fixed cylinders (203) of spring (205).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322912674.6U CN221355407U (en) | 2023-10-30 | 2023-10-30 | Pine wood nematode culture collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322912674.6U CN221355407U (en) | 2023-10-30 | 2023-10-30 | Pine wood nematode culture collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221355407U true CN221355407U (en) | 2024-07-19 |
Family
ID=91868253
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322912674.6U Active CN221355407U (en) | 2023-10-30 | 2023-10-30 | Pine wood nematode culture collection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221355407U (en) |
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2023
- 2023-10-30 CN CN202322912674.6U patent/CN221355407U/en active Active
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