CN2212591Y - Carbon dioxide controller for cell culture box - Google Patents
Carbon dioxide controller for cell culture box Download PDFInfo
- Publication number
- CN2212591Y CN2212591Y CN 94248534 CN94248534U CN2212591Y CN 2212591 Y CN2212591 Y CN 2212591Y CN 94248534 CN94248534 CN 94248534 CN 94248534 U CN94248534 U CN 94248534U CN 2212591 Y CN2212591 Y CN 2212591Y
- Authority
- CN
- China
- Prior art keywords
- gas
- feeding cylinder
- carbon dioxide
- pressure switch
- incubator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model relates to a carbon dioxide gas supply controller for cell culture boxes, which can realize without interruption gas supply and gas full utilization in gas supplying barrel. The controller is composed of a main gas supplying barrel, a spare supplying barrel, a pressure switch, an electromagnetic valve, a check valve, etc. When the gas pressure in the main supplying barrel is lower than the predetermined value of the pressure switch, the pressure switch starts the electromagnetic valve and switches the spare gas supplying barrel to supply gas; an indicator lamp indicates the gas supply state of the main supplying barrel; a guard lamp indicates the gas supply state of the spare supplying barrel. The check valve is used for ensuring that the gas in the main and the spare supplying barrels can only unidirectionally flow towards the gas inlet end of a culture box.
Description
The utility model relates to a kind of gas control equipment of bio-incubator, relates to the carbon dioxide gas member control apparatus of cell culture incubator or rather.
The carbonic acid gas constant incubator that culturing cell is used, in the biomass cells culturing process, need to supply with the carbon dioxide of incubator finite concentration, temperature, pressure, if the air feed deficiency causes gas concentration lwevel to descend, then also rapid thereupon decline of the substratum pH value in the cell culture environment caused necrocytosis, and therefore keeping stable carbonic acid gas supply status in cell cultivation process is the important factor that can cell be cultivated smoothly.
The carbon dioxide feeding mechanism of existing constant incubator comprises inflator, air shooter and is located at the tensimeter at inflator place.The inflator transfer lime directly is communicated with incubator, carbon dioxide is directly sent in the case, in the tensimeter indicator drum total gas pressure less than certain value (as 2kg '
Cm2) then dial down the inflator that the pneumatic tube of old tube renews the time.
Yet the cultivation of biomass cells needs certain hour, and above-mentioned air feeder relies on the artificial visually examine to control ventilation fully, can cause the gas supply failure on the one hand in scavenging period, influences the cultivation of cell; Owing to also have a certain amount of residual gas in the inflator, can not effectively utilize an interior gas and cause waste on the other hand.
The purpose of this utility model is the carbon dioxide gas member control apparatus of a kind of cell culture incubator of design, can not have the supply of interruptedly controlling the incubator carbon dioxide and effectively utilizes air storage in the inflator.
The carbon dioxide gas member control apparatus of the utility model cell culture incubator comprises carbon dioxide Feeding cylinder and the supply air line that is communicated with gas Feeding cylinder and incubator, it is characterized in that:
Described carbon dioxide Feeding cylinder is main Feeding cylinder;
Also comprise be hubbed at the gas pressure switch in the supply air line between carbon dioxide master Feeding cylinder and incubator and be located at gas pressure switch and incubator between vacuum breaker in the supply air line;
Also comprise the standby Feeding cylinder of a carbon dioxide, be located at the magnetic valve in the supply air line between standby Feeding cylinder and incubator and be located at magnetic valve and incubator between vacuum breaker in the supply air line;
Described gas pressure switch major joint connects power supply, and one control joint connects described electromagnetic valve coil.
Also comprise warning lamp that is connected with described pressure switch control joint and the pilot lamp that is connected with another control joint of pressure switch.
Gas master Feeding cylinder is as the pressure-controlling source of pressure switch, pressure switch is used for control and switches primary, spare inflator, and indicate the air feed state of primary, spare inflator respectively with pilot lamp and warning lamp, two vacuum breaker can guarantee that gas is only towards incubator inlet pipe uniflux in the primary, spare Feeding cylinder;
Further specify technology of the present utility model below in conjunction with the embodiment accompanying drawing
This gas control equipment of Fig. 1 master Feeding cylinder air feed view
The standby Feeding cylinder air feed of this gas control equipment of Fig. 2 view
Referring to Fig. 1, the carbon dioxide gas member control apparatus of cell culture incubator comprises a gas master Feeding cylinder 1, pressure switch 2, magnetic valve 3, auxiliary gas Feeding cylinder 4 and vacuum breaker 5,6.Pivot connects a pressure switch 2 on the supply air line 11 of main Feeding cylinder 1, and with the pressure-controlling source of main Feeding cylinder 1 as pressure switch 2.Supply air line 11 connects a vacuum breaker 5 in 7 pivots of inlet pipe of pressure switch 2 and constant incubator, one control joint C of pressure switch 2 connects pilot lamp 21, another control joint B of pressure switch 2 then connects warning lamp 22 and magnetic valve 3 respectively, the pressure switch major joint connects power supply, 3 supply air lines 41 that articulate standby Feeding cylinder 4 of magnetic valve, with the circulation of control supply air line 41, supply air line 41 is connected with vacuum breaker 6 in magnetic valve 3 and 7 other pivots of constant incubator inlet pipe.
When making cell cultures, the pressure that sets greater than pressure switch 2 when the gaseous tension of main Feeding cylinder, the action of pressure switch 2 makes the major joint A of switch inside be connected with control joint C, the main Feeding cylinder 1 of pilot lamp 21 bright expressions is in the air feed state, magnetic valve 3 and 22 of the warning lamps that link to each other with standby Feeding cylinder 4 this moment are trip condition, supply with carbonic acid gas by main Feeding cylinder 1 to constant incubator, can limit that carbon dioxide only can flow towards constant incubator inlet pipe 7 in the pipeline and be hubbed at vacuum breaker 5,6 on the supply air line 11,41, backflow phenomenon can not take place.
When the gaseous tension of main Feeding cylinder 1 is lower than the pressure that pressure switch 2 sets (as 0.2kg/cm
2), as shown in Figure 2, joint A in the pressure switch 2, the B conducting, magnetic valve 3 actions are also lighted warning lamp 22, magnetic valve 3 makes supply air line 41 form path, gas in the standby Feeding cylinder 4 is delivered to constant incubator inlet pipe 7 through supply air line 41 rapidly, the staff can understand the standby tube air feed state that is in from warning lamp 22, need in good time more change owner Feeding cylinder 1, at this moment, because the gaseous tension in main Feeding cylinder 1 supply air line 11 is far below standby Feeding cylinder 4, the gaseous tensions in the supply air line 41 are subjected to the restriction of vacuum breaker 5, supply gas only can flow towards incubator inlet pipe 7 directions, and main Feeding cylinder 1 is a trip condition.
Effect of the present utility model is: when the pressure of gas master Feeding cylinder was lower than the pressure that pressure switch sets, the pressure activated switch magnetic valve switched to by Feeding cylinder air feed for subsequent use, so there is not the supply discontinuity problem of carbon dioxide; The air feed situation that indicator lamp and warning lamp show main Feeding cylinder and Feeding cylinder for subsequent use is set, intuitively eye-catching, can remind the staff to change Feeding cylinder in good time; By the lower controlled pressure of pressure switch setting, can fully effectively utilize an interior gas.
Claims (2)
1, a kind of carbon dioxide gas member control apparatus of cell culture incubator comprises carbon dioxide Feeding cylinder and the supply air line that is communicated with gas Feeding cylinder and incubator, it is characterized in that:
Described carbon dioxide Feeding cylinder is main Feeding cylinder;
Also comprise be hubbed at the gas pressure switch in the supply air line between carbon dioxide master Feeding cylinder and incubator and be located at gas pressure switch and incubator between vacuum breaker in the supply air line;
Also comprise the standby Feeding cylinder of a carbon dioxide, be located at the magnetic valve in the supply air line between standby Feeding cylinder and incubator and be located at magnetic valve and incubator between vacuum breaker in the supply air line;
Described gas pressure switch major joint connects power supply, and one control joint connects described electromagnetic valve coil.
2, the carbon dioxide gas member control apparatus of cell culture incubator according to claim 1 is characterized in that: also comprise warning lamp that is connected with described pressure switch control joint and the pilot lamp that is connected with another control joint of pressure switch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94248534 CN2212591Y (en) | 1994-12-27 | 1994-12-27 | Carbon dioxide controller for cell culture box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94248534 CN2212591Y (en) | 1994-12-27 | 1994-12-27 | Carbon dioxide controller for cell culture box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2212591Y true CN2212591Y (en) | 1995-11-15 |
Family
ID=33853431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94248534 Expired - Fee Related CN2212591Y (en) | 1994-12-27 | 1994-12-27 | Carbon dioxide controller for cell culture box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2212591Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100460496C (en) * | 2003-12-30 | 2009-02-11 | 西北农林科技大学 | Fermentation tank for monitoring fermenting process of grape wine according to fermentation exhuast gas concentration |
| CN115248606A (en) * | 2022-06-28 | 2022-10-28 | 青岛海尔生物医疗科技有限公司 | Control method of gas supply system and gas supply system |
| CN115558593A (en) * | 2022-09-02 | 2023-01-03 | 广州市华粤行医疗科技有限公司 | Medium culture observation device and air path control method thereof |
-
1994
- 1994-12-27 CN CN 94248534 patent/CN2212591Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100460496C (en) * | 2003-12-30 | 2009-02-11 | 西北农林科技大学 | Fermentation tank for monitoring fermenting process of grape wine according to fermentation exhuast gas concentration |
| CN115248606A (en) * | 2022-06-28 | 2022-10-28 | 青岛海尔生物医疗科技有限公司 | Control method of gas supply system and gas supply system |
| CN115558593A (en) * | 2022-09-02 | 2023-01-03 | 广州市华粤行医疗科技有限公司 | Medium culture observation device and air path control method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |