CN221071538U - Culture device for proliferation and differentiation of dental pulp stem cells - Google Patents
Culture device for proliferation and differentiation of dental pulp stem cells Download PDFInfo
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- CN221071538U CN221071538U CN202322857442.5U CN202322857442U CN221071538U CN 221071538 U CN221071538 U CN 221071538U CN 202322857442 U CN202322857442 U CN 202322857442U CN 221071538 U CN221071538 U CN 221071538U
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- incubator
- dental pulp
- differentiation
- stem cells
- pulp stem
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- 210000005258 dental pulp stem cell Anatomy 0.000 title claims abstract description 35
- 230000004069 differentiation Effects 0.000 title claims abstract description 27
- 230000035755 proliferation Effects 0.000 title claims abstract description 20
- 230000007246 mechanism Effects 0.000 claims abstract description 8
- 239000011521 glass Substances 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims description 12
- 239000011324 bead Substances 0.000 claims description 7
- 230000004663 cell proliferation Effects 0.000 claims description 5
- 238000009413 insulation Methods 0.000 claims 2
- 230000012010 growth Effects 0.000 abstract description 4
- 241000700605 Viruses Species 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 description 7
- 239000011049 pearl Substances 0.000 description 6
- 238000005452 bending Methods 0.000 description 4
- 230000003321 amplification Effects 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000002449 bone cell Anatomy 0.000 description 1
- 210000003321 cartilage cell Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000003074 dental pulp Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000002894 multi-fate stem cell Anatomy 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a culture device for proliferation and differentiation of dental pulp stem cells, which comprises an incubator, wherein one side of the front end of the incubator is rotationally connected with a cabinet door through a hinge, an observation window is arranged on the cabinet door and is arranged on transparent glass, the inner wall of the front end of the incubator, which is close to the cabinet door, is rotationally connected with an observation door through the hinge, rectangular holes are formed in the top and bottom positions of the middle of the observation door, and observation mechanisms are arranged at the two rectangular holes. The utility model realizes the technical effects of effectively protecting the environment inside the incubator from external influence without opening an observation door, preventing the inside dental pulp stem cells from being influenced by external viruses and bacteria when in proliferation and differentiation, effectively protecting the quality of the dental pulp stem cells, and conveniently cleaning and observing the growth condition of the dental pulp stem cells in the internal culture dish.
Description
Technical Field
The utility model relates to the technical field of biology, in particular to a culture device for proliferation and differentiation of dental pulp stem cells.
Background
The dental pulp stem cells are multipotent stem cells mainly derived from dental pulp tissues in teeth, have self-updating and multipotent differentiation potential, can be cultured and expanded in vitro and differentiated into various cell types such as bone cells, cartilage cells, muscle cells, nerve cells and the like, have wide application prospects in the fields of regenerative medicine, tissue engineering, disease modeling and the like, and generally need to use a culture device in the proliferation and differentiation process of the dental pulp stem cells.
In the prior art, proliferation and differentiation of dental pulp stem cells are generally realized through an incubator, in the process that dental pulp stem cells are located in a culture dish for differentiation, operators observe the culture dish irregularly, the incubator is generally required to be opened during observation, the temperature loss in the incubator can be caused, and external viruses or bacteria can enter the incubator to seriously influence the culture quality of dental pulp stem cells, so that a culture device for proliferation and differentiation of dental pulp stem cells is designed.
Disclosure of utility model
The utility model aims to solve the defects in the prior art and provides a culture device for proliferation and differentiation of dental pulp stem cells.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
The utility model provides a culture apparatus for dental pulp stem cell proliferation differentiation, includes the incubator, incubator front end one side is connected with the cabinet door through the hinge rotation, be provided with the observation window on the cabinet door, and set up on the observation window in transparent glass, incubator front end inner wall is close to cabinet door department and is connected with the observation door through the hinge rotation, the rectangular hole has all been seted up with the bottom position in observation door middle part top, two rectangular hole department all is provided with viewing mechanism, viewing mechanism is including the fixed plate of fixed connection in rectangular hole department, circular observation hole has been seted up at the fixed plate middle part, fixed mounting has the convex lens in the circular observation hole, and the setting of convex lens realizes the amplification to the picture, the fixed plate is located the top department fixedly connected with bent plate of incubator inboard, bent plate bottom inclined plane department fixedly connected with reflector, the fixed plate is located the inboard bottom intermediate position fixedly connected with bottom plate of incubator, the one end fixedly mounted with lamp pearl that the bottom plate top is close to the incubator inboard.
As a further scheme of the utility model, the top of the inner side of the incubator is rotationally connected with the same rotating shaft at the middle position of the bottom, two rotating plates are fixedly connected to the circumferential surface of the rotating shaft, four placing grooves are formed in the top of each rotating plate at equal intervals in a circumferential distance, light holes are formed in the middle position of the bottom of each placing groove, and each light hole is matched with a lamp bead, so that brightness is provided for a culture dish when a user observes through the light holes.
As a further scheme of the utility model, the inner side surface of the incubator is provided with the heat preservation layer, the temperature sensor is fixedly arranged at the position, close to the top of the back end of the incubator, of the heat preservation layer, the heat preservation of the temperature in the incubator is realized, and the influence of the external temperature on the inside is reduced.
As a further scheme of the utility model, heating pipes are fixedly arranged at two ends of the bottom of the inner side of the incubator, and the temperature of the interior of the incubator is raised through the heating pipes.
As a further scheme of the utility model, a driving motor is fixedly arranged in the middle of the top of the outer wall of the incubator, the output end of the bottom of the driving motor penetrates through the incubator and is fixedly connected to the top of the rotating shaft, and the rotation of the two rotating plates is controlled through the driving motor.
As a further scheme of the utility model, a controller is fixedly arranged at the bottom of the front end of the incubator, and is respectively and electrically connected with the driving motor, the temperature sensor and the heating tube, and the temperature in the incubator is controlled through the controller.
The beneficial effects of the utility model are as follows:
The utility model comprises the following steps: due to the adoption of technical means such as a placing groove, a light hole, a fixing plate, a convex lens, a bending plate, a reflecting mirror, a bottom plate and lamp beads, when a user needs to observe a culture dish in the incubator, a cabinet door is opened, two lamp beads are opened, the light irradiates the bottom of the culture dish which is close to the light hole through the light hole which is close to the light hole, the growth condition of dental pulp stem cells in the culture dish can be clearly observed through the convex lens by virtue of the convex lens and the reflecting mirror, the problem in the background technology is effectively solved, the problem that the observation door is not required to be opened is further realized, the environment in the incubator is effectively protected from being influenced by the outside, the quality of the dental pulp stem cells is effectively protected when the dental pulp stem cells are proliferated and differentiated, and the technical effect of the growth condition of the dental pulp stem cells in the culture dish in the incubator is conveniently cleaned is observed.
The utility model comprises the following steps: through the setting of temperature sensor, heating tube and controller, realize the cooperation of temperature sensor and heating tube and controller, control the inside temperature of incubator, be favorable to the proliferation differentiation of dental pulp stem cell in the culture dish.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a culture apparatus for proliferation and differentiation of dental pulp stem cells according to the present utility model;
FIG. 2 is a schematic sectional view of an incubator for a culture apparatus for proliferation and differentiation of dental pulp stem cells according to the present utility model;
FIG. 3 is a schematic view showing the structure of a rotating plate of a culture apparatus for proliferation and differentiation of dental pulp stem cells according to the present utility model;
fig. 4 is a schematic structural view of an observation mechanism of a culture device for proliferation and differentiation of dental pulp stem cells according to the present utility model.
In the figure: 1. an incubator; 2. a cabinet door; 3. an observation window; 4. a controller; 5. a driving motor; 6. a heat preservation layer; 7. a rotating shaft; 8. a temperature sensor; 9. a rotating plate; 10. a heating tube; 11. an observation door; 12. a placement groove; 13. a light hole; 14. a fixing plate; 15. a convex lens; 16. a bending plate; 17. a reflective mirror; 18. a bottom plate; 19. and (5) a lamp bead.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other. The utility model will be described in detail below with reference to the drawings in connection with embodiments.
Referring to fig. 1-4, a culture apparatus for proliferation and differentiation of dental pulp stem cells, including incubator 1, incubator 1 front end one side is connected with cabinet door 2 through the hinge rotation, be provided with observation window 3 on cabinet door 2, and set up in transparent glass on the observation window 3, incubator 1 front end inner wall is close to cabinet door 2 department and is connected with observation door 11 through the hinge rotation, the rectangular hole has all been seted up to observation door 11 middle part top and bottom position, two rectangular hole departments all are provided with viewing mechanism, viewing mechanism is including fixed connection fixed plate 14 in rectangular hole department, circular observation hole has been seted up at the fixed plate 14 middle part, fixed mounting has convex lens 15 in the circular observation hole, convex lens 15's setting realizes the amplification to the picture, be convenient for the user observe, fixed plate 14 is located the inboard top department fixedly connected with bending plate 16 of incubator 1, bending plate 16 bottom inclined plane department fixedly connected with reflector 17, the setting of reflector 17 makes the user can observe the culture dish top situation, fixed plate 14 is located the inboard bottom middle position fixedly connected with bottom plate 18 of incubator 1, the fixed mounting bead 19 that the inboard one end of bottom plate 18 is close to incubator 1.
In this embodiment, the same pivot 7 is rotated and is connected with bottom intermediate position at the inboard top of incubator 1, and two rotating plates 9 of pivot 7 circumference fixed surface are connected with, and equal circumference equidistance in every rotating plate 9 top leaves and is equipped with four standing grooves 12, and light trap 13 has all been seted up to every standing groove 12 bottom intermediate position, and every light trap 13 all with lamp pearl 19 looks adaptation, the setting of light trap 13, the light of the lamp pearl 19 of being convenient for passes from the culture dish bottom, provides certain luminance for user's observation.
In this embodiment, incubator 1 inboard surface is provided with heat preservation 6, and the setting of heat preservation 6 prevents that the inside temperature of incubator 1 from scattering and losing, and heat preservation 6 is close to incubator 1 back end top department fixed mounting has temperature sensor 8.
In this embodiment, the heating tubes 10 are fixedly installed at two ends of the bottom inside the incubator 1, and the temperature inside the incubator 1 is controlled by the two heating tubes 10, so that a good environment is provided for stem cell differentiation.
In this embodiment, the middle position of incubator 1 outer wall top fixed mounting has driving motor 5, and the output of driving motor 5 bottom runs through incubator 1 fixed connection at the top of pivot 7, through driving motor 5, realizes driving two rotation plates 9 rotation, the user of being convenient for observes.
In this embodiment, a controller 4 is fixedly installed at the bottom of the front end of the incubator 1, and the controller 4 is electrically connected with a driving motor 5, a temperature sensor 8 and a heating tube 10 respectively.
Working principle: this kind of a culture apparatus for dental pulp stem cell proliferation differentiation, when using, will be used for dental pulp stem cell proliferation differentiation culture dish to place respectively in the standing groove 12 on two rotating plates 9, close observation door 11 and cabinet door 2, through the cooperation of temperature sensor 8 and heating tube 10 and controller 4, control the inside temperature of incubator 1, be favorable to the dental pulp stem cell proliferation differentiation in the culture dish, when the user needs to observe the culture dish in incubator 1 inside, open cabinet door 2, open two lamp pearls 19, the opening of lamp pearl 19, make the light shine the bottom of the culture dish that is close to with it through the light trap 13 that is close to, through the effect of convex lens 15 and reflector 17, make the user see the dental pulp stem cell growth situation in the culture dish clearly through convex lens 15, when the culture dish that needs to change the observation, drive rotating plate 9 through pivot 7 rotates, make the culture dish that is located lamp pearl 19 top replace, the user continues to observe, and the culture dish inside the incubator 1 can not contact with the inside of incubator 1, the quality of the inside can not be contacted with the outside and the virus growth, the external quality of the inside of incubator is protected.
Spatially relative terms, such as "above … …," "above … …," "upper surface on … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial location relative to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the exemplary term "above … …" may include both orientations "above … …" and "below … …". The device may also be positioned in other different ways (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (6)
1. The utility model provides a culture apparatus for dental pulp stem cell proliferation differentiation, includes incubator (1), its characterized in that, incubator (1) front end one side is connected with cabinet door (2) through the hinge rotation, be provided with observation window (3) on cabinet door (2), and be provided with on observation window (3) in transparent glass, incubator (1) front end inner wall is close to cabinet door (2) department and is connected with observation door (11) through the hinge rotation, rectangular hole has all been seted up at observation door (11) middle part top and bottom position, two rectangular hole department all is provided with viewing mechanism, viewing mechanism is including fixed connection fixed plate (14) in rectangular hole department, circular observation hole has been seted up at fixed plate (14) middle part, fixed mounting has convex lens (15) in the circular observation hole, fixed plate (14) are located the inboard top department fixedly connected with bent plate (16) of incubator (1), bent plate (16) bottom inclined plane department fixedly connected with reflector (17), fixed plate (14) are located incubator (1) inboard bottom intermediate position fixedly connected with bottom plate (18), be close to one end bead (19) of inner side lamp bottom plate (1).
2. The culture device for proliferation and differentiation of dental pulp stem cells according to claim 1, wherein the middle position of the inner top and the bottom of the incubator (1) is rotationally connected with the same rotating shaft (7), two rotating plates (9) are fixedly connected to the circumferential surface of the rotating shaft (7), four placing grooves (12) are formed in the top of each rotating plate (9) at equal intervals in a circumferential equidistant manner, light holes (13) are formed in the middle position of the bottom of each placing groove (12), and each light hole (13) is matched with a lamp bead (19).
3. The culture device for proliferation and differentiation of dental pulp stem cells according to claim 1, wherein an insulation layer (6) is arranged on the inner side surface of the incubator (1), and a temperature sensor (8) is fixedly arranged on the insulation layer (6) close to the top of the back end of the incubator (1).
4. The culture device for proliferation and differentiation of dental pulp stem cells according to claim 1, wherein heating pipes (10) are fixedly installed at both ends of the inner bottom of the incubator (1).
5. The culture device for proliferation and differentiation of dental pulp stem cells according to claim 1, wherein a driving motor (5) is fixedly arranged in the middle of the top of the outer wall of the incubator (1), and the output end of the bottom of the driving motor (5) penetrates through the incubator (1) and is fixedly connected to the top of the rotating shaft (7).
6. The culture device for proliferation and differentiation of dental pulp stem cells according to claim 1, wherein a controller (4) is fixedly installed at the bottom of the front end of the incubator (1), and the controller (4) is electrically connected with a driving motor (5), a temperature sensor (8) and a heating tube (10) respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322857442.5U CN221071538U (en) | 2023-10-24 | 2023-10-24 | Culture device for proliferation and differentiation of dental pulp stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322857442.5U CN221071538U (en) | 2023-10-24 | 2023-10-24 | Culture device for proliferation and differentiation of dental pulp stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221071538U true CN221071538U (en) | 2024-06-04 |
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ID=91267223
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322857442.5U Active CN221071538U (en) | 2023-10-24 | 2023-10-24 | Culture device for proliferation and differentiation of dental pulp stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221071538U (en) |
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2023
- 2023-10-24 CN CN202322857442.5U patent/CN221071538U/en active Active
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