CN220938228U

CN220938228U - Tumor implantation mechanism

Info

Publication number: CN220938228U
Application number: CN202321881374.XU
Authority: CN
Inventors: 孙泉; 徐楠楠; 孟晓丹
Original assignee: Beijing Zhaoyan New Drug Research Center Co ltd
Current assignee: Beijing Zhaoyan New Drug Research Center Co ltd
Priority date: 2023-07-18
Filing date: 2023-07-18
Publication date: 2024-05-14
Anticipated expiration: 2033-07-18

Abstract

The utility model relates to a tumor implantation mechanism, which comprises an implantation tube and an implantation rod matched with the implantation tube, wherein the implantation tube comprises a tube part, an axially-through tube cavity is arranged in the tube part, and the shape of the tube cavity is columnar; the implantation rod comprises a rod part and a limiting part, wherein the rod part is columnar in shape and is matched with the outer diameter of the rod part and the inner diameter of the lumen; the limiting part is connected to the tail end of the rod part; when the head end of the implantation rod is inserted into the lumen from the tail of the implantation tube and the limiting part abuts against the tail end of the implantation tube, the head end of the implantation rod is flush with the head end of the implantation tube. Can plant the tumour fritter subcutaneous more easily to can prevent that the tumour fritter from being kneaded the condition emergence of breaking in the planting process, promote the success rate that the tumour was planted, be convenient for establish the tumour model.

Description

Tumor implantation mechanism

Technical Field

The utility model belongs to the field of inoculation devices, and particularly relates to a tumor implantation mechanism.

Background

The establishment of tumor models using mice is an important method of modern oncology research. Mouse tumor models can be categorized into subcutaneous and in situ tumor formation according to the location of inoculation. The subcutaneous tumorigenesis is simple to operate, is convenient to observe, and compared with in-situ tumorigenesis, the pathophysiology of the tumor is not significantly different, so that the application in practical research is more common. The PDX (patient-derivedtumorxenografts) model is a very important model tool in the field of accurate medical treatment, and is characterized in that fresh tumor tissues of patients are transplanted to an immunodeficiency mouse after being simply treated, the environment provided by the mouse is relied on for growth, the microenvironment and basic characteristics of primary tumors are reserved, and then the curative effect of a research medicament is evaluated through mouse administration. The model provides a good in vivo model for researching tumors. The current conventional PDX model construction steps are as follows: ① The tumor specimens of the patients are collected and completely soaked in the double-resistant PRMI1640 culture medium at the temperature of 0 ℃. ② The animal is completely soaked after being washed by the double-antibody PRMI1640 culture medium at the temperature of 0 ℃ and is brought back to the animal house for operation. ③ Tumor tissue was trimmed to 2 x 3mm ³ and washed 3 times in the above medium. The skin of the two sides of the nude mice with the age of 4-6 weeks under anesthesia is provided with a small opening of about 3mm respectively, a small pocket-shaped space is separated, and the trimmed tumor small tissues are planted under the skin to suture the skin incision. ④ A drop of 100 x diab solution was placed in the incision to prevent wound infection. ⑤ The condition of the transplanted tumor is observed once a week, and the transplanted tumor starts growing to 1-2cm ³ after about 3-5 weeks.

When the tumor small blocks are planted under the skin, forceps are used for clamping the tumor small blocks, and then the tumor small blocks are planted under the skin; however, in the process of clamping the tumor small pieces by forceps, the situation that the tumor small pieces are crushed can be caused, especially at the moment of planting the tumor small pieces subcutaneously, the operator cannot observe the tumor state at the moment, false marks that the tumor small pieces are planted successfully can occur by mistake, and therefore the curative effect of the research medicine cannot be evaluated through administration of mice.

Disclosure of utility model

In view of the above, the utility model provides a tumor implantation mechanism, which can easily implant tumor small blocks under the skin, can prevent the tumor small blocks from being pinched during the implantation process, improves the success rate of tumor implantation, and is convenient for establishing a tumor model.

The technical scheme adopted by the utility model is as follows:

the tumor implantation mechanism comprises an implantation tube and an implantation rod matched with the implantation tube, wherein the implantation tube comprises a tube part, a tube cavity which is axially penetrated is arranged in the tube part, and the shape of the tube cavity is columnar;

The implantation rod comprises a rod part and a limiting part, wherein the rod part is columnar in shape and is matched with the outer diameter of the rod part and the inner diameter of the lumen; the limiting part is connected to the tail end of the rod part;

When the head end of the implantation rod is inserted into the lumen from the tail of the implantation tube and the limiting part abuts against the tail end of the implantation tube, the head end of the implantation rod is flush with the head end of the implantation tube.

Preferably, the head end of the rod portion is hemispherical.

Preferably, the implant tube further comprises a matching part, wherein the matching part is connected to the tail end of the tube part, and the tube cavity penetrates through the matching part;

The head end of the implantation rod is inserted into the lumen from the tail of the implantation tube, and when the limiting part abuts against the matching part, the head end of the implantation rod is flush with the head end of the implantation tube.

Preferably, the mating portion is provided with a connection hole, the connection hole is a through hole, the pipe portion passes through the connection hole, and the tail end of the pipe portion is flush with the tail end of the mating portion.

Preferably, an external thread is arranged on the matching part, an annular matching sleeve is formed on one side of the limiting part, facing the head end of the rod part, the rod part passes through the matching sleeve, an internal thread is arranged in the matching sleeve, and the internal thread is in threaded fit with the external thread;

The limiting part is propped against the matching part, and when the internal thread is completely matched with the external thread, the head end of the implantation rod is flush with the head end of the implantation tube.

Preferably, in the axial direction, the length of the internal thread is equal to the length of the external thread, and the length of the internal thread and the external thread is 0.5 cm to 1cm.

Preferably, the matching part comprises an equal-diameter section and a conical section which are connected with each other, the conical section is positioned at the head end side of the equal-diameter section, the outer diameter of the equal-diameter section is equal to the maximum outer diameter of the conical section, and the outer diameter of the conical section gradually becomes smaller from the tail end to the head end;

The appearance of spacing portion is the column, the external diameter of spacing portion is equal with the external diameter of constant diameter section.

Preferably, friction lines are arranged on the outer surfaces of the matching part and the limiting part.

The utility model has the beneficial effects that:

When a tumor small block is planted, the tumor small block is placed into the head end of the tube part of the implantation tube, then the head end of the implantation tube is stretched into the skin, the head end of the implantation rod is inserted into the tube cavity from the tail end of the implantation tube, the head end of the implantation rod is stretched to the head end position of the tube part at the rear side, the tumor small block is pushed forward until the limiting part is propped against the tail end of the implantation tube, at the moment, the implantation rod can not be pushed forward relative to the implantation tube, and the short enough of the implantation rod is just flush with the head end of the implantation tube, so that the tumor small block is just pushed out of the implantation tube and planted under the skin; in the above process, because the tumor small blocks are positioned in the lumen, and the tube part is of a rigid structure, the tumor small blocks cannot be subjected to external pressure in the process of planting the tumor small blocks, and the situation that the tumor small blocks are cracked cannot occur, so that the success rate of tumor planting is improved, and a tumor model is convenient to establish.

Drawings

The above and other objects, features and advantages of the present utility model will become more apparent from the following description of embodiments of the present utility model with reference to the accompanying drawings, in which:

FIG. 1 is a cross-sectional view of an implant;

FIG. 2 is a cross-sectional view of an implant rod;

Fig. 3 is a cross-sectional view of the implant rod as it is inserted into the implant tube.

In the figure: 1. an implant; 2. implanting a rod;

11. a tube section; 12. a mating portion; 13. a lumen; 121. an isodiametric section; 122. a conical section;

21. a stem portion; 22. a limit part; 221. and (5) matching the sleeve.

Detailed Description

The present utility model is described below based on examples, but the present utility model is not limited to only these examples. In the following detailed description of the present utility model, certain specific details are set forth in order to avoid obscuring the present utility model, and in order to avoid obscuring the present utility model, well-known methods, procedures, flows, and components are not presented in detail.

Moreover, those of ordinary skill in the art will appreciate that the drawings are provided herein for illustrative purposes and that the drawings are not necessarily drawn to scale.

Unless the context clearly requires otherwise, throughout the description and the claims, the words "comprise", "comprising", and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is, it is the meaning of "including but not limited to".

In the description of the present utility model, it should be understood that the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.

As shown in fig. 3, based on the operation time, the side close to the inside of the mouse body is the front side, the side close to the operator is the back side, the front side corresponds to the head end side, and the back side corresponds to the tail end side.

Referring to fig. 1-3, the utility model provides a tumor implantation mechanism for tumor implantation, which comprises an implantation tube 1 and an implantation rod 2 matched with the implantation tube 1, wherein the implantation tube 1 comprises a tube part 11, a tube cavity 13 which is axially penetrated is arranged in the tube part 11, and the shape of the tube cavity 13 is columnar.

The implantation rod 2 comprises a rod part 21 and a limiting part 22, wherein the rod part 21 is columnar in shape and is matched with the outer diameter of the rod part 21 and the inner diameter of the tube cavity 13; the limiting part 22 is connected to the tail end of the rod part 21.

When the head end of the implant rod 2 is inserted into the lumen 13 from the tail of the implant tube 1 and the limit part 22 abuts against the tail end of the implant tube 1, the head end of the implant rod 2 is flush with the head end of the implant tube 1.

When the tumor implantation mechanism is used for implanting tumors, a tumor small block is firstly placed into the head end of the tube part 11 of the implantation tube 1, then the head end of the implantation tube 1 is stretched into the skin, the head end of the implantation rod 2 is inserted into the tube cavity 13 from the tail end of the implantation tube 1, the head end of the implantation rod 2 is stretched to the head end position of the tube part 11 at the rear side, the tumor small block is pushed forward until the limit part 22 is propped against the tail end of the implantation tube 1, at the moment, the implantation rod 2 can not be pushed forward relative to the implantation tube 1 any more, at the moment, the short enough of the implantation rod 2 is just flush with the head end of the implantation tube 1, so that the tumor small block is just pushed out of the implantation tube 1 and is implanted into the skin; in the above process, since the tumor small block is positioned in the lumen 13, the tube part 11 is of a rigid structure, so that the tumor small block is not subjected to external pressure in the process of planting the tumor small block, the situation that the tumor small block is cracked is avoided, the success rate of tumor planting is improved, and the establishment of a tumor model is facilitated.

The tube part 11 is of a slender columnar structure, and the inside of the tube part is provided with the tube cavity 13 which is penetrated in the axial direction, so that the front end (corresponding to the head end) and the rear end (corresponding to the tail end) of the tube part 11 of the tube cavity 13 are penetrated, and thus small tumor blocks can be placed in the front end part of the tube cavity 13, and the small tumor blocks are protected in the tumor implantation process.

The rod 21 may be a solid metal rod, which may be inserted into the lumen 13 from the rear end of the tube 11, and as the portion into which the rod 21 is inserted increases, the front end of the rod 21 contacts the tumor mass and pushes the tumor mass forward when pushing the front end of the lumen 13, thereby pushing the tumor mass out of the tube 11 and achieving the purpose of tumor implantation.

Furthermore, the setting of the stop 22 can limit the length of insertion of the implant rod 2 into the lumen 13 such that the front end of the implant rod 2 can be just flush with the front end of the tube portion 11 to just push the tumor mass out of the implant tube 1.

Further, the head end of the rod 21 is hemispherical. When the implantation rod 2 pushes the tumor small blocks, the head end of the rod part 21 is contacted with the tumor small blocks, and the head end of the rod part 21 is arranged into a hemispherical shape, so that the tumor small blocks can be prevented from being clamped in the gap between the rod part 21 and the pipe part 11, and the complete tumor small blocks are ensured to be implanted to the preset positions.

Of course, in other embodiments, the distal end of the stem 21 is cylindrical.

The implant 1 further comprises a mating portion 12, the mating portion 12 is connected to the tail end of the tube portion 11, and the lumen 13 penetrates through the mating portion 12.

When the head end of the implant rod 2 is inserted into the lumen 13 from the tail of the implant tube 1 and the limit part 22 abuts against the matching part 12, the head end of the implant rod 2 is flush with the head end of the implant tube 1.

The provision of the mating portion 12 allows an operator to grasp the implant tube 1 more easily, thereby facilitating implantation of the tumor mass.

Moreover, when the tumor mass is pushed by the implantation rod 2, the engaging portion 12 can limit the limit portion 22, ensuring that the front end of the rod portion 21 is at most flush with the front end of the tube portion 11 without protruding from the front end of the tube portion 11.

The matching part 12 is provided with external threads, one side of the limiting part 22 facing the head end of the rod part 21 is provided with an annular matching sleeve 221, the rod part 21 passes through the matching sleeve 221, the matching sleeve 221 is internally provided with internal threads, and the internal threads are in threaded fit with the external threads.

The limiting part 22 abuts against the matching part 12, and when the internal thread is completely matched with the external thread, the head end of the implantation rod 2 is flush with the head end of the implantation tube 1.

When the limiting part 22 forms the matching sleeve 221 forward, the rod part 21 is inserted into the pipe part 11, the implantation rod 2 is pushed forward until the limiting part 22 is in contact with the matching part 12, at this time, the front end of the rod part 21 is still positioned in the pipe part 11, and then the limiting part 22 is rotated, so that the external threads are matched with the internal threads, the implantation rod 2 is slowly pushed forward until the external threads are completely matched with the internal threads, at this time, the front end of the implantation rod 2 is flush with the front end of the implantation pipe 1, so that the tumor small blocks are just pushed out, and the tumor small blocks are planted at a slower speed in the process of planting the tumor small blocks, so that the planting effectiveness of the tumor small blocks is ensured.

Further, in the axial direction, the length of the internal thread is equal to that of the external thread, and the length of the internal thread and the external thread is 0.5cm to 1cm.

When the tumor mass is placed into the front end of the lumen 13, the tumor mass is positioned within 0.5cm of the front end, ensuring that the internal threads mate with the external threads during pushing of the tumor mass.

The mating portion 12 includes an equal-diameter section 121 and a tapered section 122 connected to each other, the tapered section 122 is located at a head end side of the equal-diameter section 121, an outer diameter of the equal-diameter section 121 is equal to a maximum outer diameter of the tapered section 122, and an outer diameter of the tapered section 122 gradually becomes smaller from a tail end to a head end.

The outer shape of the limiting part 22 is columnar, and the outer diameter of the limiting part 22 is equal to the outer diameter of the constant diameter section 121.

Since the outer diameter of the limiting part 22 is equal to the outer diameter of the equal-diameter section 121, when the limiting part 22 is propped against the matching part 12, the limiting part 22 and the matching part 12 form a whole from the appearance, and the appearance is more attractive; the tapered section 122 in the mating portion 12 is provided to facilitate the operator to hold the mating portion 12.

Friction lines are arranged on the outer surfaces of the matching part 12 and the limiting part 22. The arrangement of the friction lines facilitates the operator to control the implant 1 through the mating part 12 and the implant rod 2 through the limiting part 22, especially in the embodiment with mating of the internal thread and the external thread, to rotate the limiting part 22.

It will be understood that the above-described embodiments are merely illustrative and not restrictive, and that all obvious or equivalent modifications and substitutions to the details given above may be made by those skilled in the art without departing from the underlying principles of the utility model, are intended to be included within the scope of the appended claims.

Claims

1. The tumor implantation mechanism is characterized by comprising an implantation tube and an implantation rod matched with the implantation tube, wherein the implantation tube comprises a tube part, an axially-through tube cavity is arranged in the tube part, and the shape of the tube cavity is columnar;

2. The tumor implantation mechanism according to claim 1, wherein the head end of the stem portion is hemispherical.

3. The tumor implantation mechanism according to claim 1, wherein the implantation tube further comprises a mating portion, the mating portion being connected to a trailing end of the tube portion, and the lumen passing through the mating portion;

4. The tumor implantation mechanism according to claim 3, wherein the mating portion is provided with a connection hole, the connection hole is a through hole, the tube portion passes through the connection hole, and a tail end of the tube portion is flush with a tail end of the mating portion.

5. The tumor implantation mechanism according to claim 3, wherein the matching part is provided with external threads, an annular matching sleeve is formed on one side of the limiting part facing the head end of the rod part, the rod part passes through the matching sleeve, the matching sleeve is internally provided with internal threads, and the internal threads are in threaded fit with the external threads;

6. The tumor implantation mechanism according to claim 5, wherein the length of the internal thread is equal to the length of the external thread in the axial direction and the length of both is 0.5-1cm.

7. The tumor implantation mechanism according to claim 3, wherein the mating portion comprises an equal diameter section and a tapered section connected to each other, the tapered section being located on a head end side of the equal diameter section, an outer diameter of the equal diameter section being equal to a maximum outer diameter of the tapered section, and an outer diameter of the tapered section being gradually smaller in a direction from a tail end to a head end;

8. The tumor implantation mechanism according to claim 3, wherein friction lines are provided on the outer surfaces of the mating portion and the limiting portion.