CN220846160U

CN220846160U - Culture device

Info

Publication number: CN220846160U
Application number: CN202322349435.4U
Authority: CN
Inventors: 肖帅锋; 刘芝庆; 黄亚雄
Original assignee: Hunan Antai Kangcheng Biotechnology Co ltd
Current assignee: Hunan Antai Kangcheng Biotechnology Co ltd
Priority date: 2023-08-30
Filing date: 2023-08-30
Publication date: 2024-04-26
Anticipated expiration: 2033-08-30

Abstract

The application relates to the technical field of cell culture, in particular to a culture device, which comprises a box body, a water-cooling row, a first fan assembly, a bearing assembly and a host, wherein the water-cooling row is provided with a heat absorption end positioned in the box body and a heat emission end positioned outside the box body; the first fan assembly is arranged in the box body and is used for driving gas in the box body to pass through the heat absorbing end of the water cooling exhaust; the bearing assembly is arranged in the box body and comprises a supporting plate and a culture dish arranged on the supporting plate; the host computer sets up on the box, and the host computer is connected with bearing assembly electricity, and this culture apparatus can carry out effectual auxiliary temperature control to the box inside for the inside temperature of box maintains in required within range, guarantees that the cell normally grows, and the experiment goes on smoothly.

Description

Culture device

Technical Field

The application relates to the technical field of cell culture, in particular to a culture device.

Background

Cell culture (cellculture) refers to a method of simulating in vitro an in vivo environment (sterile, suitable temperature, humidity, certain nutritional conditions, etc.), allowing cells to survive, grow, proliferate and maintain their primary structure and function. Cell culture is also known as cell cloning technology, a formal biological term being cell culture technology. Cell culture is an essential process for the whole biotechnology, or for one of the biological cloning techniques, and is itself a large-scale cloning of cells.

The existing cell culture box does not have the function of active temperature control, and the cell culture device inside has higher power and can accumulate more heat, so that the temperature inside the culture box is continuously increased, the growth of cells in the culture box is influenced, and the smooth implementation of experiments is influenced.

Disclosure of utility model

The application aims to provide a culture device which can effectively assist in controlling the temperature in a box body so as to maintain the temperature in the box body within a required range and ensure normal growth of cells and smooth experiment.

To this end, an embodiment of the present application provides a culture apparatus including: a case; the water cooling row is provided with a heat absorption end positioned in the box body and a heat release end positioned outside the box body; the first fan assembly is arranged in the box body and is used for driving gas in the box body to pass through the heat absorbing end of the water cooling exhaust; the bearing assembly is arranged in the box body and comprises a supporting plate and a culture dish arranged on the supporting plate; and the host is arranged on the box body and is electrically connected with the bearing assembly.

In one possible implementation, the first fan assembly drives the gas of the tank to form a circulating gas flow that flows through the culture dish.

In one possible implementation manner, at least one partition board is transversely arranged in the box body, the at least one partition board divides the interior of the box body into at least two accommodating spaces, communication holes for communicating the two adjacent accommodating spaces are formed in the partition board, and circulating air flows circularly through the communication holes in the partition board.

In one possible implementation, the heat absorbing end of the water-cooled row is disposed on the partition, and the first fan assembly is disposed on the heat absorbing end of the water-cooled row.

In one possible implementation manner, the water-cooled row is provided with a plurality of heat absorbing ends, and the plurality of heat absorbing ends are respectively arranged on the plurality of partition plates.

In one possible implementation, the first fan assembly is disposed between the baffle and the heat absorbing end of the water cooled row.

In one possible implementation, the culture device further includes a third fan assembly disposed on a top or bottom surface of the space in the case for accelerating air circulation in the case.

In one possible implementation, the bottom of the internal space of the tank is provided with a water tank; the water tank is provided with a cover body which is provided with a plurality of vent holes; the culture device is provided with a pipeline, and one end of the pipeline is communicated with the water tank; the other end of the pipeline is connected with a water pump arranged outside the box body.

In one possible implementation, the culture device further comprises: the temperature sensor is arranged in the box body and used for detecting the internal temperature of the box body; and the control center is electrically connected with the temperature sensor and the water-cooling row and controls the working state of the water-cooling row according to the temperature output by the temperature sensor.

In one possible implementation, the culture device further comprises a second fan assembly arranged at the heat release end, and the second fan assembly is used for driving external air to pass through the heat release end.

In one possible implementation, the heat absorbing end includes a first heat exchanger, the heat releasing end includes a second heat exchanger and a water pump, and a liquid circulation loop is formed between the first heat exchanger and the second heat exchanger through the water pump.

In one possible implementation, a bidirectional joint is arranged on the box body, one end of the bidirectional joint is connected with the heat absorbing end of the water cooling row, and the other end of the bidirectional joint is connected with the heat releasing end of the water cooling row.

In one possible implementation, the pallet is provided with mounting slots for mounting the culture dish.

According to the culture device provided by the embodiment of the application, the temperature in the box body is absorbed through the heat absorption end, the temperature is discharged out of the box body through the heat release end, and the air in the box body is driven to flow through the first fan assembly, so that the heat in the box body can be fully exchanged with the heat absorption end, the effective auxiliary temperature control in the box body can be realized, the internal temperature of the box body is maintained in a required range, and the normal growth of cells in a culture dish on a tray and the smooth experiment are ensured.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art. In addition, in the drawings, like parts are designated with like reference numerals and the drawings are not drawn to actual scale.

FIG. 1 is a schematic perspective view showing a culture apparatus according to an embodiment of the present application;

FIG. 2 is a schematic plan view of a culture apparatus according to an embodiment of the present application;

FIG. 3 shows a schematic diagram of a water cooled row, a first fan assembly, and a second fan assembly according to an embodiment of the present application;

fig. 4 shows a schematic view of the structure of fig. 3 at another angle.

Reference numerals illustrate:

1. A case; 11. a partition plate; 12. an accommodating space; 13. a two-way joint;

2. Water-cooling rows; 21. a heat absorbing end; 211. a first heat exchanger; 22. a heat release end; 221. a second heat exchanger; 222. a water pump;

3. A first fan assembly;

4. a carrier assembly; 41. a supporting plate; 42. a culture dish;

5. A host; 6. a temperature sensor; 7. a control center; 8. a second fan assembly.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

FIG. 1 is a schematic perspective view showing a culture apparatus according to an embodiment of the present application; FIG. 2 is a schematic plan view of a culture apparatus according to an embodiment of the present application; FIG. 3 shows a schematic diagram of a water cooled row, a first fan assembly, and a second fan assembly according to an embodiment of the present application; fig. 4 shows a schematic view of the structure of fig. 3 at another angle.

As shown in fig. 1 to 4, an embodiment of the present application provides a culture apparatus including: the device comprises a box body 1, a water cooling row 2, a first fan assembly 3, a bearing assembly 4 and a host machine 5.

The water-cooled row 2 has a heat absorbing end 21 located inside the tank 1 and a heat releasing end 22 located outside the tank 1.

The first fan assembly 3 is disposed in the box 1 and is used for driving the gas in the box 1 to pass through the heat absorbing end 21 of the water cooling exhaust 2.

The bearing assembly 4 is arranged in the box 1, and the bearing assembly 4 comprises a supporting plate 41 and a culture dish 42 arranged on the supporting plate 41.

The host 5 is disposed on the case 1, and the host 5 is electrically connected to the carrying component 4.

In the application, the temperature inside the box body 1 is absorbed through the heat absorbing end 21, the outside of the box body 1 is discharged through the heat releasing end 22, and the first fan assembly 3 drives the gas inside the box body 1 to flow, so that the heat inside the box body 1 can exchange heat with the heat absorbing end 21 sufficiently, the effective auxiliary temperature control inside the box body 1 can be realized, the internal temperature of the box body 1 is maintained in a required range, and the normal growth of cells in the culture dish 42 on the tray and the smooth experiment are ensured.

Specifically, the host computer 5 is electrically connected to the carrier assembly 4, and is used for controlling the culture dish 42 on the support plate 41 to work, so that the culture dish 42 provides a required environment for the growth of cells.

In some embodiments, the first fan assembly 3 drives the gas in the tank 1 into a circulating gas flow that flows through the culture dish 42.

In the application, the first fan assembly 3 drives the gas of the box body 1 to form a circulating gas flow, when the circulating gas flow flows through the culture dish 42, the heat at the culture dish 42 is carried to the heat absorption end 21 of the water cooling row 2, the heat absorption end 21 exchanges heat, and the low-temperature gas after heat exchange enters the circulating gas flow again to cool the culture dish 42, so that the uniformity of the temperature in the box body 1 is ensured.

Specifically, the electrical components on the culture dish 42 also emit certain heat in the working process, the heat is concentrated near the culture dish 42, and the embodiment of the application drives the gas in the box body 1 to circularly flow through the first fan assembly 3, so that the heat at the culture dish 42 can be taken away timely, and the efficient temperature control at the culture dish 42 is realized.

In some embodiments, at least one partition 11 is transversely disposed in the interior of the case 1, the at least one partition 11 divides the interior of the case 1 into at least two accommodation spaces 12, and communication holes for communicating adjacent two accommodation spaces 12 are disposed in the partition 11, and the circulating air flows circularly through the communication holes in the partition 11.

According to the application, the inner space of the box body 1 is divided into a plurality of accommodating spaces 12 by the partition plates 11 transversely arranged in the box body 1, and the two adjacent accommodating spaces 12 are communicated through the communication holes on the partition plates 11, so that the gas in the box body 1 can circularly flow, and the bearing components 4 are placed on the partition plates 11, so that the circulating gas flow cools the culture dishes 42 of the bearing components 4 when circularly flowing, a plurality of bearing components 4 can be placed in the box body 1, and meanwhile, the effective temperature control of the bearing components 4 is ensured.

Further, the plurality of partitions 11 may be arranged in the case 1 at equal intervals or non-equal intervals in the vertical direction, depending on the height of the carrying assembly 4.

The baffle 11 in the application can adopt a metal screen plate, and the metal screen plate is provided with communication holes; the partition 11 may be a metal plate, and a plurality of vertically oriented communication holes are arranged on the metal plate in an array manner so as to allow the air flow in the box 1 to circulate.

In some embodiments, the heat absorbing end 21 of the water-cooled row 2 is disposed on the partition 11, and the first fan assembly 3 is disposed on the heat absorbing end 21 of the water-cooled row 2.

According to the application, the heat absorption end 21 of the water cooling row 2 is directly arranged on the partition plate 11, the heat absorption end 21 of the water cooling row 2 can directly exchange heat with the partition plate 11, so that the partition plate 11 keeps relatively low temperature, the partition plate 11 exchanges heat with the bearing component 4, the bearing component 4 is cooled, meanwhile, the heat absorption end 21 of the water cooling row 2 exchanges heat with the partition plate 11, so that the partition plate 11 is at low temperature, the temperature of the horizontal plane of the partition plate 11 in the box body 1 can be at low temperature, and the temperature control effect in the box body 1 is improved; by arranging the first fan assembly 3 on the heat absorbing end 21 of the water-cooled row 2, the air flow can be ensured to fully flow through the heat absorbing end 21 of the water-cooled row 2, and the heat exchanging effect at the heat absorbing end 21 is improved.

In some embodiments, the water-cooled row 2 has a plurality of heat absorbing ends 21, and the plurality of heat absorbing ends 21 are respectively disposed on the plurality of partition boards 11.

In the application, each partition plate 11 can be cooled by arranging one heat absorbing end 21 or a plurality of heat absorbing ends 21 on each partition plate 11, so that gases with different heights in the box body 1 can be cooled, and the temperature control precision of the bearing assembly 4 on each partition plate 11 is ensured.

In some embodiments, the first fan assembly 3 is disposed between the baffle 11 and the heat absorbing end 21 of the water cooled row 2.

Specifically, the first fan assembly 3 may also be disposed on the partition 11 opposite to the heat absorbing end 21 of the water-cooled row 2, where the first fan assembly 3 drives the gas to flow through the heat absorbing end 21 of the water-cooled row 2, so that the gas can exchange heat at the heat absorbing end 21 of the water-cooled row 2.

In some embodiments, the culture device further comprises a third fan assembly disposed on a top or bottom surface of the interior space of the housing for accelerating air circulation within the housing.

According to the application, the third fan can accelerate air circulation in the box body, so that the temperature control effect in the box body is further improved, and the uniformity of the temperature in the box body is ensured.

In some embodiments, a water tank is arranged at the bottom of the inner space of the box body; the water tank is provided with a cover body which is provided with a plurality of vent holes; the culture device is provided with a pipeline, and one end of the pipeline is communicated with the water tank; the other end of the pipeline is connected with a water pump arranged outside the box body.

In the application, the inside of the box body can be humidified through the water tank.

In some embodiments, the culture device further comprises: a temperature sensor 6 provided inside the case 1 for detecting the internal temperature of the case 1; and a control center 7 electrically connected with the temperature sensor 6 and the water-cooled bar 2, wherein the control center 7 controls the working state of the water-cooled bar 2 according to the temperature output by the temperature sensor 6.

In the application, the temperature in the box body 1 is detected by the temperature sensor 6, the detected temperature information is sent to the control center 7, and the control center 7 controls the working state of the water cooling row 2 according to the temperature information, so that the accurate temperature control in the box body 1 is realized. Specifically, the control center 7 controls the circulation frequency of the cooling liquid between the heat absorbing end 21 and the heat releasing end 22 of the water-cooled exhaust 2, when the temperature in the box 1 is too high, the cooling effect is improved by increasing the circulation frequency of the cooling liquid between the heat absorbing end 21 and the heat releasing end 22, and along with the reduction of the temperature in the box 1, the circulation frequency of the cooling liquid between the heat absorbing end 21 and the heat releasing end 22 can be correspondingly reduced, so that the temperature in the box 1 is stabilized at the set temperature.

In some embodiments, the culture device further comprises a second fan assembly 8 disposed at the heat release end 22, and the second fan assembly 8 is configured to drive external air through the heat release end 22.

In the application, the second fan assembly 8 drives the external air to flow through the heat release end 22 of the water cooling row 2, and the external cold air exchanges heat with the heat release end 22, so that the heat release end 22 is cooled, and the cooled cooling liquid at the heat release end 22 returns to the heat release end 21 for cooling the inside of the box body 1, so that the cooling effect of the inside of the box body 1 is further improved.

As shown in fig. 3-4, in some embodiments, the heat absorbing end 21 includes a first heat exchanger 211, the heat releasing end 22 includes a second heat exchanger 221, and a water pump 222, and a liquid circulation loop is formed between the first heat exchanger 211 and the second heat exchanger 221 by the water pump 222.

In the application, the water pump 222 drives the cooling liquid between the first heat exchanger 211 and the second heat exchanger 221 to circularly flow, the cooling liquid exchanges heat with the gas in the box body 1 at the first heat exchanger 211, the interior of the box body 1 is cooled, the warmed cooling liquid flows to the second heat exchanger 221, the cooling liquid exchanges heat with the external air at the second heat exchanger 221 and is changed into low-temperature cooling liquid again, and the low-temperature cooling liquid flows to the first heat exchanger 211 to cool the interior of the box body 1.

As shown in fig. 1, in some embodiments, the box 1 is provided with a bidirectional connector 13, one end of the bidirectional connector 13 is connected to the heat absorbing end 21 of the water-cooled bar 2, and the other end is connected to the heat releasing end 22 of the water-cooled bar 2.

In the application, the bidirectional joint 13 is a water pipe, and the cooling liquid between the heat absorption end 21 and the heat release end 22 of the water-cooled exhaust 2 flows through the water pipe, specifically, the bidirectional joint 13 penetrates through the box body 1, so that the tightness of the box body 1 is ensured.

Optionally, the bidirectional joint 13 can also enter the box body 1 through the opening of the box body 1, and the inner side of the door plate of the box body 1 is in butt joint with the box body 1 through a sealing ring to ensure the tightness of the bidirectional joint 13.

In some embodiments, the tray 41 is provided with mounting slots for mounting the culture dish 42.

In the present application, the mounting and fixing effects of the culture dish 42 are ensured by the mounting grooves on the supporting plate 41.

In the embodiment of the application, the carbon dioxide sensor and the carbon dioxide generating device are also arranged on the box body 1, carbon dioxide gas is filled into the box body 1 through the carbon dioxide generating device, and the concentration of carbon dioxide in the box body 1 is detected through the carbon dioxide sensor, so that the concentration value of the carbon dioxide in the box body 1 is ensured, and a required gas environment is provided for cell culture.

In the embodiment of the application, a humidity sensor is further arranged in the box body 1, the humidity inside the box body 1 is detected through the humidity sensor, and the humidity inside the box body 1 is adjusted by matching with a humidifying device.

The temperature inside the box body 1 is absorbed by the heat absorption end 21, the outside of the box body 1 is discharged by the heat absorption end 22, and the air inside the box body 1 is driven to flow by the first fan assembly 3, so that the heat inside the box body 1 can exchange heat with the heat absorption end 21 sufficiently, the effective auxiliary temperature control inside the box body 1 can be realized, the internal temperature of the box body 1 is maintained in a required range, and the normal growth of cells in the culture dish 42 on the tray and the smooth experiment are ensured.

It should be noted that references in the specification to "one embodiment," "an example embodiment," "some embodiments," etc., indicate that the embodiment described may include a particular feature, structure, or characteristic, but every embodiment may not necessarily include the particular feature, structure, or characteristic. Moreover, such phrases are not necessarily referring to the same embodiment. Furthermore, when a particular feature, structure, or characteristic is described in connection with an embodiment, it is submitted that it is within the knowledge of one skilled in the art to effect such feature, structure, or characteristic in connection with other embodiments whether or not explicitly described.

It should be readily understood that the terms "on … …", "above … …" and "above … …" in this disclosure should be interpreted in the broadest sense so that "on … …" means not only "directly on something" but also includes "on something" with intermediate features or layers therebetween, and "above … …" or "above … …" includes not only the meaning "on something" or "above" but also the meaning "above something" or "above" without intermediate features or layers therebetween (i.e., directly on something).

Further, spatially relative terms, such as "below," "beneath," "above," "over," and the like, may be used herein for ease of description to describe one element or feature's relationship to another element or feature as illustrated. Spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. The device may have other orientations (rotated 90 degrees or at other orientations), and the spatially relative descriptors used herein interpreted accordingly.

It should be noted that in this document, relational terms such as "first" and "second" and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present application, and not for limiting the same; although the application has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the application.

Claims

1. A culture device, comprising:

A case (1);

A water-cooled row (2), wherein the water-cooled row (2) is provided with a heat absorbing end (21) positioned inside the box body (1) and a heat releasing end (22) positioned outside the box body (1);

The first fan assembly (3) is arranged in the box body (1) and is used for driving gas in the box body (1) to pass through the heat absorption end (21) of the water cooling row (2);

The bearing assembly (4) is arranged in the box body (1), and the bearing assembly (4) comprises a supporting plate (41) and a culture dish (42) arranged on the supporting plate (41); and

The host (5) is arranged on the box body (1), and the host (5) is electrically connected with the bearing assembly (4).

2. Culture device according to claim 1, characterized in that the first fan assembly (3) brings the gas of the tank (1) into a circulating gas flow, which flows through the culture dish (42).

3. Culture device according to claim 2, characterized in that the interior of the tank (1) is laterally provided with at least one partition (11), the at least one partition (11) dividing the interior of the tank (1) into at least two accommodation spaces (12), the partition (11) being provided with communication holes for communicating two adjacent accommodation spaces (12), the circulating air flow circulating through the communication holes in the partition (11).

4. A culture device according to claim 3, wherein the heat absorbing end (21) of the water cooled row (2) is arranged on the partition (11), and the first fan assembly (3) is arranged on the heat absorbing end (21) of the water cooled row (2).

5. The culture apparatus according to claim 4, wherein the water-cooled row (2) has a plurality of the heat absorbing ends (21), and the plurality of heat absorbing ends (21) are respectively provided on the plurality of partition plates (11).

6. A culture device according to claim 3, wherein the first fan assembly (3) is arranged between the partition (11) and the heat absorbing end (21) of the water cooled row (2).

7. A culture device according to claim 3, further comprising a third fan assembly provided on the top or bottom surface of the interior space of the housing (1) for accelerating the ventilation in the housing (1).

8. A culture device according to claim 3, wherein the bottom of the internal space of the tank (1) is provided with a water tank; the water tank is provided with a cover body, and the cover body is provided with a plurality of vent holes; the culture device is provided with a pipeline, and one end of the pipeline is communicated with the water tank; the other end of the pipeline is connected with a water pump arranged outside the box body (1).

9. The culture device of claim 1, further comprising:

The temperature sensor (6) is arranged inside the box body (1) and is used for detecting the internal temperature of the box body (1); and

The control center (7) is electrically connected with the temperature sensor (6) and the water cooling row (2), and the control center (7) controls the working state of the water cooling row (2) according to the temperature output by the temperature sensor (6).

10. The culture device according to claim 1, further comprising a second fan assembly (8) arranged at the heat release end (22), the second fan assembly (8) being adapted to drive external air through the heat release end (22).

11. Culture device according to claim 1, characterized in that the heat absorbing end (21) comprises a first heat exchanger (211), the heat releasing end (22) comprises a second heat exchanger (221) and a water pump (222), a liquid circulation loop being formed between the first heat exchanger (211) and the second heat exchanger (221) by the water pump (222).

12. Culture device according to claim 1, characterized in that the tank (1) is provided with a two-way joint (13), one end of the two-way joint (13) being connected to the heat absorbing end (21) of the water-cooled row (2) and the other end being connected to the heat releasing end (22) of the water-cooled row (2).

13. Culture device according to claim 1, characterized in that the carrier (41) is provided with mounting grooves for mounting the culture dishes (42).