CN220812480U - Cell in-vitro fluid shearing stimulation culture device - Google Patents

Abstract

The utility model discloses an external fluid shearing stimulation culture device for cells, which comprises a rectangular culture dish, wherein the culture dish comprises a substrate and a fixed cover, a fixed groove and a perfusion groove are formed in the substrate from top to bottom, the two sides of the fixed groove and the perfusion groove are in a step shape, a substrate for cell culture is paved at the bottom of the perfusion groove, the thickness of the substrate is smaller than the depth of the perfusion groove, a glass cover is placed on the step of the fixed groove, and a perfusion cell interlayer is formed between the bottom of the glass cover and the substrate; a lower convex part is arranged below the fixed cover, and the lower convex part is inserted into the fixed groove and is pressed above the glass cover; the two ends of the matrix are respectively provided with interlayer small holes corresponding to the interlayer of the perfusion small chamber, one side of the interlayer small holes is connected with a liquid inlet pipe, a peristaltic pump is arranged on the liquid inlet pipe, the other side of the interlayer small holes is connected with a liquid outlet pipe, and a vacuum pump is arranged on the liquid outlet pipe. The utility model realizes that fluid shear force and pressure stimulus are simultaneously given to cells by forming a perfusion cell interlayer and pouring and sucking culture solution, thereby simulating the real environment in vivo.

Description

A cell in vitro fluid shear stimulation culture device

Technical Field

The utility model relates to the technical field of molecular biology research equipment, in particular to an in vitro fluid shear stimulation culture device for cells.

Background technique

Vascular endothelial cells are subject to various mechanical forces under the physiological and pathological environment of the human body. Among them, the important mechanical forces generated by liquids are fluid shear force and pressure (blood pressure). The effects of these mechanical forces on the normal physiological functions of vascular endothelial cells and the regulation of cell pathological changes by changes in fluid shear force have received more and more widespread attention. In order to better study the effects of fluid shear force on the function of vascular endothelial cells, various cell in vitro fluid shear devices have emerged, such as a cell fluid shear stress generating device disclosed in Chinese utility model patent CN210945633U (authorization announcement date: 2020.07.07), and a cell culture device for studying fluid shear force disclosed in Chinese utility model patent CN215162808U (authorization announcement date: 2021.12.14). The above devices can all achieve the effect of applying fluid shear stress in cell tissue culture, but the existing devices can only provide fluid shear force stimulation, cannot provide pressure stimulation, and cannot fully simulate the real situation in the body. In addition, specially designed electric devices and control systems are required to control the magnitude of fluid shear force (flow rate) and stimulation mode (constant current stimulation or pulse stimulation). The overall system is relatively complex and has a high entry threshold, which restricts the widespread development of research related to fluid shear force.

Utility Model Content

In order to solve the above-mentioned deficiencies in the prior art, the utility model provides a simple, low-cost in vitro fluid shear stimulation cell culture device which can simultaneously provide controllable fluid shear force and pressure to cells.

In order to achieve the above technical purpose, the technical solution adopted by the utility model is:

A cell in vitro fluid shear stimulation culture device comprises a rectangular culture dish, the culture dish comprises a base and a fixed cover, a fixed groove and a perfusion groove are opened from top to bottom in the base, the two sides of the fixed groove are stepped with the perfusion groove, a substrate for cell culture is laid at the bottom of the perfusion groove, the thickness of the substrate is less than the depth of the perfusion groove, a glass cover is placed on the step of the fixed groove, and a perfusion chamber sandwich is formed between the bottom of the glass cover and the substrate; a lower convex part is arranged below the fixed cover, the lower convex part is inserted into the fixed groove and pressed above the glass cover; sandwich holes are respectively arranged at the positions of the perfusion chamber sandwich at both ends of the base corresponding to the positions of the perfusion chamber sandwich, one side of the sandwich hole is connected to a liquid inlet conduit, a peristaltic pump is arranged on the liquid inlet conduit to input culture fluid into the perfusion chamber sandwich, and the other side of the sandwich hole is connected to a liquid outlet conduit, a vacuum pump is arranged on the liquid outlet conduit to suck the culture fluid in the perfusion chamber sandwich.

Preferably, the substrate is rectangular, and a plurality of substrates are arranged side by side.

Preferably, there are eight substrates in total.

Furthermore, it also includes a clamping block, which is U-shaped and clamped on the outer periphery of the culture dish.

Preferably, the base and the fixing cover are made of transparent material.

Preferably, the base and the fixing cover are made of acrylic material.

Furthermore, rubber blocks are respectively provided at both ends of the perfusion groove, and through holes are provided at the locations of the rubber blocks corresponding to the interlayer holes; and rubber strips are provided in the side walls on both sides of the fixing groove along the length direction.

The utility model is used to culture cells carried on a substrate, which is laid in a perfusion tank. By perfusing and aspirating the culture fluid, fluid shear force and pressure stimulation are simultaneously applied to the cells, thereby simulating the real environment in the body. By setting multiple substrates, cells can be grouped and multiple groups of cells can be cultured at the same time. The utility model has a compact structure and can be placed in a cell incubator for culture as needed, achieving long-term fluid shear force and pressure stimulation.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

FIG1 is an exploded schematic diagram of an embodiment of the utility model;

FIG2 is a schematic longitudinal section view of an embodiment of the utility model;

FIG3 is a schematic cross-sectional view of an embodiment of the present utility model;

FIG. 4 is a top view of the base (including rubber blocks and rubber strips).

Figure numerals: 1-fixed cover, 2-base, 3-substrate, 4-glass cover, 5-perfusion chamber interlayer, 6-interlayer hole, 7-liquid inlet conduit, 8-peristaltic pump, 9-liquid outlet conduit, 10-vacuum pump, 11-block, 12-rubber block, 13-rubber strip.

Detailed ways

To make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the application claimed for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians of the art without making creative work are within the scope of protection of the present application.

The present embodiment discloses a simple multi-group cell in vitro fluid shear stimulation culture device, as shown in Figures 1 to 4, comprising a rectangular culture dish, the culture dish comprising a base 2 and a fixed cover 1, the base 2 is provided with a fixed groove and a perfusion groove from top to bottom, the two sides of the fixed groove are stepped with the perfusion groove, a substrate 3 for cell culture is laid at the bottom of the perfusion groove, the thickness of the substrate 3 is less than the depth of the perfusion groove, a glass cover 4 is placed on the step of the fixed groove, and a perfusion chamber sandwich 5 is formed between the bottom of the glass cover 4 and the substrate 3; a lower convex portion is provided below the fixed cover 1, the lower convex portion is inserted into the fixed groove and pressed above the glass cover 4; sandwich small chambers are respectively provided at the positions of the two ends of the base 2 corresponding to the perfusion chamber sandwich 5 The hole 6 of the interlayer on one side is connected to the liquid inlet conduit 7, and a peristaltic pump 8 is arranged on the liquid inlet conduit 7 to input the culture fluid into the interlayer 5 of the perfusion chamber. The hole 6 of the interlayer on the other side is connected to the liquid outlet conduit 9, and a vacuum pump 10 is arranged on the liquid outlet conduit 9 to suck the culture fluid in the interlayer 5 of the perfusion chamber. The flow rate and peristaltic mode (such as constant current stimulation or pulse stimulation) of the culture fluid input are controlled by the peristaltic pump 8, and the suction force of the vacuum pump 10 is adjusted to control the rate at which the culture fluid flows out. When the outflow rate of the culture fluid is less than the inflow rate, a certain fluid shear force and pressure can be applied to the cells on the substrate 3, thereby simulating the real environment in the body and achieving the same and controllable fluid shear force and pressure stimulation to the cells at the same time.

Preferably, the substrate 3 is rectangular, and a rectangular cover glass can be used as a surface lining as required for conventional cell culture. The substrate 3 can be provided in multiple pieces and laid side by side in the perfusion tank. The length of the perfusion tank and the glass cover can also be customized according to the number of substrates 3 to be placed as needed. For example, in this embodiment, a total of eight substrates are provided, the first and tail two are used for balancing the buffer fluid, and the middle 6 are used for formal experiments. Grouping processing can be performed on the substrate as required, such as silencing or overexpressing a certain gene, or giving drug stimulation, so as to achieve the same fluid shear force and pressure stimulation for multiple groups of cells at the same time. The matrix 2 and the fixed cover 1 are made of transparent materials, such as acrylic materials, which are convenient for overall experimental observation.

Furthermore, it also includes a clamping block 11, which is U-shaped, and at least two clamping blocks 11 are provided, which are clamped on the outer periphery of the culture dish and are used to clamp and fix the cover 1 and the base 2 to play a fixing role.

Furthermore, rubber blocks 12 are provided at both ends of the perfusion groove, and through holes are opened at the positions of the rubber blocks 12 corresponding to the interlayer holes 6 or punctured by needles to facilitate the input of culture fluid; rubber strips 13 are provided in the side walls of both sides of the fixed groove to seal and prevent liquid from leaking outward under pressure.

Of course, the present invention may have many other embodiments. Without departing from the spirit and essence of the present invention, technicians familiar with the field may make various corresponding changes and deformations based on the present invention, but these corresponding changes and deformations should all fall within the scope of protection of the claims attached to the present invention.

Claims (7)

1. An in vitro fluid shear stimulation culture device, includes rectangular culture dish, its characterized in that: the culture dish comprises a substrate (2) and a fixed cover (1), wherein a fixed groove and a perfusion groove are formed in the substrate (2) from top to bottom, two sides of the fixed groove and the perfusion groove are in a step shape, a substrate (3) for cell culture is paved at the bottom of the perfusion groove, the thickness of the substrate (3) is smaller than the depth of the perfusion groove, a glass cover (4) is placed on the step of the fixed groove, and a perfusion small chamber interlayer (5) is formed between the bottom of the glass cover (4) and the substrate (3); a lower convex part is arranged below the fixed cover (1), and the lower convex part is inserted into the fixed groove and is pressed above the glass cover (4); the two ends of the matrix (2) are respectively provided with an interlayer small hole (6) corresponding to the perfusion small chamber interlayer (5), one side of the interlayer small hole (6) is connected with a liquid inlet conduit (7), a peristaltic pump (8) is arranged on the liquid inlet conduit (7) to input culture liquid into the perfusion small chamber interlayer (5), the other side of the interlayer small hole (6) is connected with a liquid outlet conduit (9), and a vacuum pump (10) is arranged on the liquid outlet conduit (9) to suck the culture liquid in the perfusion small chamber interlayer (5).

2. The in vitro fluid shear stimulation culture device of claim 1, wherein: the substrate (3) is rectangular, and a plurality of substrates (3) are arranged side by side.

3. The in vitro fluid shear stimulation culture device of claim 2, wherein: eight substrates (3) are arranged in total.

4. The in vitro fluid shear stimulation culture device of claim 1, wherein: still include fixture block (11), fixture block (11) are the U-shaped, and fixture block (11) presss from both sides and establishes at the culture dish periphery.

5. The in vitro fluid shear stimulation culture device of claim 1, wherein: the base body (2) and the fixed cover (1) are made of transparent materials.

6. The in vitro fluid shear stimulation culture device of claim 5, wherein: the base body (2) and the fixed cover (1) are made of acrylic materials.

7. The in vitro fluid shear stimulation culture device of claim 1, wherein: rubber blocks (12) are respectively arranged at two ends of the perfusion groove, and through holes are formed in the positions, corresponding to the interlayer small holes (6), of the rubber blocks (12); rubber strips (13) are arranged in the side walls of the two sides of the fixing groove along the length direction.