CN220812480U - Cell in-vitro fluid shearing stimulation culture device - Google Patents
Cell in-vitro fluid shearing stimulation culture device Download PDFInfo
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- CN220812480U CN220812480U CN202322546912.6U CN202322546912U CN220812480U CN 220812480 U CN220812480 U CN 220812480U CN 202322546912 U CN202322546912 U CN 202322546912U CN 220812480 U CN220812480 U CN 220812480U
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- 239000012530 fluid Substances 0.000 title claims abstract description 30
- 230000000638 stimulation Effects 0.000 title claims abstract description 24
- 238000000338 in vitro Methods 0.000 title claims description 13
- 238000010008 shearing Methods 0.000 title abstract description 3
- 230000010412 perfusion Effects 0.000 claims abstract description 38
- 239000000758 substrate Substances 0.000 claims abstract description 37
- 239000011229 interlayer Substances 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 239000011521 glass Substances 0.000 claims abstract description 14
- 238000004113 cell culture Methods 0.000 claims abstract description 6
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 6
- 239000011159 matrix material Substances 0.000 claims abstract description 4
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 238000001727 in vivo Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 16
- 230000000694 effects Effects 0.000 description 5
- 210000003556 vascular endothelial cell Anatomy 0.000 description 3
- 238000011160 research Methods 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000004938 stress stimulation Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an external fluid shearing stimulation culture device for cells, which comprises a rectangular culture dish, wherein the culture dish comprises a substrate and a fixed cover, a fixed groove and a perfusion groove are formed in the substrate from top to bottom, the two sides of the fixed groove and the perfusion groove are in a step shape, a substrate for cell culture is paved at the bottom of the perfusion groove, the thickness of the substrate is smaller than the depth of the perfusion groove, a glass cover is placed on the step of the fixed groove, and a perfusion cell interlayer is formed between the bottom of the glass cover and the substrate; a lower convex part is arranged below the fixed cover, and the lower convex part is inserted into the fixed groove and is pressed above the glass cover; the two ends of the matrix are respectively provided with interlayer small holes corresponding to the interlayer of the perfusion small chamber, one side of the interlayer small holes is connected with a liquid inlet pipe, a peristaltic pump is arranged on the liquid inlet pipe, the other side of the interlayer small holes is connected with a liquid outlet pipe, and a vacuum pump is arranged on the liquid outlet pipe. The utility model realizes that fluid shear force and pressure stimulus are simultaneously given to cells by forming a perfusion cell interlayer and pouring and sucking culture solution, thereby simulating the real environment in vivo.
Description
Technical Field
The utility model relates to the technical field of molecular biology research equipment, in particular to an in-vitro fluid shear stimulation culture device.
Background
Vascular endothelial cells are subjected to various mechanical forces in physiological and pathological environments of the human body. Among the important mechanical forces generated by liquids are fluid shear forces and pressure (blood pressure), the effects of these mechanical forces on the normal physiological functions of vascular endothelial cells, and the regulation of pathological changes in cells by fluid shear force changes have received increasing attention. To better investigate the effect of fluid shear on vascular endothelial cell function, various in vitro fluid shear devices have been developed, such as a cell fluid shear stress generating device disclosed in chinese patent No. CN210945633U (grant publication date: 2020.07.07), and a cell culture device for studying fluid shear force, such as that disclosed in chinese patent No. CN215162808U (grant publication date: 2021.12.14). The device can realize the effect of applying fluid shear stress in cell tissue culture, but the existing device only can apply fluid shear stress stimulation, can not provide pressure stimulation and can not completely simulate the in-vivo real situation. And the electric device and the control system are specially designed, so that the control of the magnitude (flow velocity) and the stimulation mode (constant-current stimulation or pulse stimulation) of the fluid shear force is realized, the whole system is complex, the admission threshold is high, and the wide development of related researches of the fluid shear force is restricted.
Disclosure of utility model
In order to solve the defects in the prior art, the utility model provides a simple and low-cost in-vitro fluid shear stimulation culture device capable of simultaneously giving controllable fluid shear force and pressure to cells.
In order to achieve the technical purpose, the utility model adopts the following technical scheme:
The culture dish comprises a substrate and a fixed cover, wherein a fixed groove and a perfusion groove are formed in the substrate from top to bottom, two sides of the fixed groove and the perfusion groove are in a step shape, a substrate for cell culture is paved at the bottom of the perfusion groove, the thickness of the substrate is smaller than the depth of the perfusion groove, a glass cover is placed on the step of the fixed groove, and a perfusion cell interlayer is formed between the bottom of the glass cover and the substrate; a lower convex part is arranged below the fixed cover, and the lower convex part is inserted into the fixed groove and is pressed above the glass cover; the two ends of the matrix are respectively provided with interlayer small holes corresponding to the interlayer of the perfusion small chamber, one side of the interlayer small holes is connected with a liquid inlet pipe, a peristaltic pump is arranged on the liquid inlet pipe to input culture liquid into the interlayer of the perfusion small chamber, the other side of the interlayer small holes is connected with a liquid outlet pipe, and a vacuum pump is arranged on the liquid outlet pipe to suck the culture liquid in the interlayer of the perfusion small chamber.
Preferably, the substrate is rectangular, and a plurality of substrates are arranged side by side.
Preferably, the substrate is provided with eight pieces in total.
Further, the culture dish further comprises a clamping block, wherein the clamping block is U-shaped and clamped on the periphery of the culture dish.
Preferably, the base body and the fixed cover are made of transparent materials.
Preferably, the base body and the fixed cover are made of acrylic materials.
Further, rubber blocks are respectively arranged at two ends of the perfusion groove, and through holes are formed in the positions, corresponding to the interlayer small holes, of the rubber blocks; rubber strips are arranged in the side walls of the two sides of the fixing groove along the length direction.
The utility model is used for culturing cells loaded on the substrate, the substrate is paved in the perfusion groove, and the simultaneous application of fluid shear force and pressure stimulation to the cells is realized by perfusion and suction of culture solution, so that the real in-vivo environment is simulated. The multi-block substrate is arranged, so that the cells can be grouped, and meanwhile, a plurality of groups of cells are cultured. The utility model has small structure, can be put into a cell incubator for culture according to the requirement, and realizes long-time fluid shear force and pressure stimulation.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is an exploded view of an embodiment of the present utility model;
FIG. 2 is a schematic longitudinal cross-section of an embodiment of the present utility model;
FIG. 3 is a schematic cross-sectional view of an embodiment of the present utility model;
FIG. 4 is a top view of the substrate (including rubber blocks, strips).
Reference numerals: 1-fixed cover, 2-base body, 3-substrate, 4-glass cover, 5-perfusion cell interlayer, 6-interlayer small hole, 7-liquid inlet conduit, 8-peristaltic pump, 9-liquid outlet conduit, 10-vacuum pump, 11-fixture block, 12-rubber block and 13-rubber strip.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. The components of the embodiments of the present application generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the application, as presented in the figures, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The embodiment discloses a simple and convenient multi-group in-vitro fluid shear stimulation culture device, as shown in figures 1-4, comprising a rectangular culture dish, wherein the culture dish comprises a base body 2 and a fixed cover 1, a fixed groove and a perfusion groove are formed in the base body 2 from top to bottom, two sides of the fixed groove and the perfusion groove are in a step shape, a substrate 3 for cell culture is paved at the bottom of the perfusion groove, the thickness of the substrate 3 is smaller than the depth of the perfusion groove, a glass cover 4 is placed on the step of the fixed groove, and a perfusion cell interlayer 5 is formed between the bottom of the glass cover 4 and the substrate 3; a lower convex part is arranged below the fixed cover 1, and is inserted into the fixed groove and is pressed above the glass cover 4; the two ends of the matrix 2 are respectively provided with interlayer small holes 6 corresponding to the perfusion small chamber interlayer 5, one side of the interlayer small holes 6 is connected with a liquid inlet pipe 7, a peristaltic pump 8 is arranged on the liquid inlet pipe 7 to input culture liquid into the perfusion small chamber interlayer 5, the other side of the interlayer small holes 6 is connected with a liquid outlet pipe 9, a vacuum pump 10 is arranged on the liquid outlet pipe 9 to suck the culture liquid in the perfusion small chamber interlayer 5, the peristaltic pump 8 is used for controlling the input flow rate and peristaltic mode (such as constant flow stimulation or pulse stimulation) of the culture liquid, the suction of the vacuum pump 10 is regulated and controlled to control the outflow rate of the culture liquid, when the outflow rate of the culture liquid is smaller than the inflow rate, a certain fluid shear force and pressure can be applied to cells on the substrate 3, so that the stimulation of the same and controllable fluid shear force and pressure can be simultaneously given to the cells is realized.
Preferably, the substrate 3 is rectangular, a rectangular cover glass can be used as a surface lining according to requirements for conventional cell culture, and a plurality of substrates 3 can be arranged and laid in the perfusion groove side by side. The length of the perfusion groove and the glass cover can also be customized according to the number of substrates 3 which are required to be placed, for example, in the embodiment, eight substrates are arranged in total, the first and the last substrates are used for balancing buffer liquid, the middle 6 substrates are used for formal experiments, grouping treatment such as silencing or over-expressing a certain gene or giving medicine stimulation can be performed on the substrates according to requirements, and therefore, the same fluid shear force and pressure stimulation can be simultaneously applied to multiple groups of cells. The base body 2 and the fixed cover 1 are made of transparent materials, such as acrylic materials, so that the whole experiment observation is convenient.
Further, the culture dish further comprises clamping blocks 11, the clamping blocks 11 are U-shaped, at least two clamping blocks 11 are arranged and clamped on the periphery of the culture dish, and the culture dish is used for clamping and fixing the cover 1 and the base body 2 to achieve a fixing effect.
Further, rubber blocks 12 are respectively arranged at two ends of the perfusion groove, and through holes are formed in positions, corresponding to the interlayer small holes 6, of the rubber blocks 12, or the culture solution is conveniently input through needling and puncturing; rubber strips 13 are arranged in the side walls of the two sides of the fixing groove, so that the sealing and liquid-proof effects are achieved, and the outward leakage of liquid under pressure is avoided.
Of course, the present utility model is capable of other various embodiments and its several details are capable of modification and variation in light of the present utility model by one skilled in the art without departing from the spirit and scope of the utility model as defined in the appended claims.
Claims (7)
1. An in vitro fluid shear stimulation culture device, includes rectangular culture dish, its characterized in that: the culture dish comprises a substrate (2) and a fixed cover (1), wherein a fixed groove and a perfusion groove are formed in the substrate (2) from top to bottom, two sides of the fixed groove and the perfusion groove are in a step shape, a substrate (3) for cell culture is paved at the bottom of the perfusion groove, the thickness of the substrate (3) is smaller than the depth of the perfusion groove, a glass cover (4) is placed on the step of the fixed groove, and a perfusion small chamber interlayer (5) is formed between the bottom of the glass cover (4) and the substrate (3); a lower convex part is arranged below the fixed cover (1), and the lower convex part is inserted into the fixed groove and is pressed above the glass cover (4); the two ends of the matrix (2) are respectively provided with an interlayer small hole (6) corresponding to the perfusion small chamber interlayer (5), one side of the interlayer small hole (6) is connected with a liquid inlet conduit (7), a peristaltic pump (8) is arranged on the liquid inlet conduit (7) to input culture liquid into the perfusion small chamber interlayer (5), the other side of the interlayer small hole (6) is connected with a liquid outlet conduit (9), and a vacuum pump (10) is arranged on the liquid outlet conduit (9) to suck the culture liquid in the perfusion small chamber interlayer (5).
2. The in vitro fluid shear stimulation culture device of claim 1, wherein: the substrate (3) is rectangular, and a plurality of substrates (3) are arranged side by side.
3. The in vitro fluid shear stimulation culture device of claim 2, wherein: eight substrates (3) are arranged in total.
4. The in vitro fluid shear stimulation culture device of claim 1, wherein: still include fixture block (11), fixture block (11) are the U-shaped, and fixture block (11) presss from both sides and establishes at the culture dish periphery.
5. The in vitro fluid shear stimulation culture device of claim 1, wherein: the base body (2) and the fixed cover (1) are made of transparent materials.
6. The in vitro fluid shear stimulation culture device of claim 5, wherein: the base body (2) and the fixed cover (1) are made of acrylic materials.
7. The in vitro fluid shear stimulation culture device of claim 1, wherein: rubber blocks (12) are respectively arranged at two ends of the perfusion groove, and through holes are formed in the positions, corresponding to the interlayer small holes (6), of the rubber blocks (12); rubber strips (13) are arranged in the side walls of the two sides of the fixing groove along the length direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322546912.6U CN220812480U (en) | 2023-09-19 | 2023-09-19 | Cell in-vitro fluid shearing stimulation culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322546912.6U CN220812480U (en) | 2023-09-19 | 2023-09-19 | Cell in-vitro fluid shearing stimulation culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220812480U true CN220812480U (en) | 2024-04-19 |
Family
ID=90697984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322546912.6U Active CN220812480U (en) | 2023-09-19 | 2023-09-19 | Cell in-vitro fluid shearing stimulation culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220812480U (en) |
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2023
- 2023-09-19 CN CN202322546912.6U patent/CN220812480U/en active Active
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