CN220745909U - Novel fed-batch fermentation feeding bottle - Google Patents
Novel fed-batch fermentation feeding bottle Download PDFInfo
- Publication number
- CN220745909U CN220745909U CN202321749185.7U CN202321749185U CN220745909U CN 220745909 U CN220745909 U CN 220745909U CN 202321749185 U CN202321749185 U CN 202321749185U CN 220745909 U CN220745909 U CN 220745909U
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- bottle
- silicone tube
- feeding
- pipe clamp
- bottle body
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- 238000000855 fermentation Methods 0.000 title claims abstract description 52
- 230000004151 fermentation Effects 0.000 title claims abstract description 52
- 229920001296 polysiloxane Polymers 0.000 claims abstract description 46
- 229920001169 thermoplastic Polymers 0.000 claims abstract description 22
- 239000004416 thermosoftening plastic Substances 0.000 claims abstract description 22
- 239000000463 material Substances 0.000 claims description 9
- 230000001502 supplementing effect Effects 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 6
- 230000037431 insertion Effects 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 12
- 239000001963 growth medium Substances 0.000 abstract description 6
- 230000002572 peristaltic effect Effects 0.000 description 17
- 239000007921 spray Substances 0.000 description 10
- 239000012526 feed medium Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000019522 cellular metabolic process Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000000078 claw Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a novel fed batch fermentation feeding bottle, in particular to the technical field of fermentation feeding bottles, which comprises a transparent bottle body, wherein a feeding component is arranged on the bottle body and comprises a bottle cap which is arranged on the bottle body and is in threaded connection with the bottle body, and one side of the top of the bottle cap is provided with a first silicone tube for guiding flow. According to the utility model, the feeding component is arranged, the first pipe clamp and the tail end of the thermoplastic pipe are in an open state until the pipeline at the tank opening of the fermentation tank, and the second pipe clamp is in a closed state, so that split flow control is realized, the second pipe clamp and the pipe clamp on the pipeline at the tail end of the thermoplastic pipe until the tank opening of the fermentation tank are closed, and feeding is finished, so that the risk caused by contact between a feeding culture medium and gas generated in the fermentation tank is reduced, and the risk of leakage or pollution caused by long-time storage of liquid in a large section of pipeline is reduced.
Description
Technical Field
The utility model relates to the technical field of fermentation feeding bottles, in particular to a novel fed-batch fermentation feeding bottle.
Background
Fed-batch fermentation refers to a fermentation technique in which a certain amount of material is fed to a fermentation system in some way during a microbial batch fermentation, but fermentation broth is not continuously discharged outwards, and is a fermentation technique that is intermediate between batch fermentation and continuous fermentation.
The utility model patent with the patent application number of CN2021212092225. X discloses a novel fed-batch fermentation system, which belongs to the technical field of microbial fermentation and comprises: the fermentation tank comprises a feeding bottle, a pump inlet pipeline, a conveying pump, a feeding conveying pipeline, a spray head assembly and a fermentation tank, wherein a feeding culture medium is contained in the feeding bottle, one end of the pump inlet pipeline is connected with the feeding bottle, the other end of the pump inlet pipeline is connected with an inlet of the conveying pump, an outlet of the conveying pump is connected with the feeding conveying pipeline, the other end of the feeding conveying pipeline is connected with the spray head assembly, and the spray head assembly is arranged in a cover body of the fermentation tank; the spray head assembly comprises a spray head body, a pipeline joint, a spray head core, a spray head piece and a spray head cap, wherein spray holes are formed in the spray head piece;
when the structure is used, the driver rotor alternately extrudes and releases the elastic conveying hose to pump fluid, the pressure pipe gap between the peristaltic pump head and the driver claw is finely adjusted through a ratchet wheel or other conventional known modes so as to adapt to hoses with different wall thicknesses or realize fine adjustment of pressure, the peristaltic pump is adopted as a conveying pump to convey the feed medium, but the pipeline is filled with the high-difficulty feed medium for a long time, precipitation is easy to occur, the feed at the tank opening is contacted with cell metabolism tail gas in the fermentation tank for a long time, the property of the feed medium is easy to change, the fermentation result is influenced, the pipeline is installed or detached on the peristaltic pump in a liquid state during each subsequent feed, the pipeline is extruded, the risk of liquid leakage is caused due to overlarge pressure in the pipeline, the peristaltic pump is reversely started, the operation is complicated and easy to miss, the feed medium is reversely pumped back into the feed medium bottle so that the cell metabolism tail gas (such as carbon dioxide gas) in the fermentation tank communicated with the feed medium enters the feed medium bottle together, and the property of the feed medium is changed.
Disclosure of Invention
The technical scheme of the utility model aims at the technical problems of the prior art solution and provides a solution which is obviously different from the prior art. In order to overcome the defects in the prior art, the utility model provides a novel fed-batch fermentation feeding bottle, which aims to solve the problems in the prior art.
The utility model is realized in such a way that the utility model provides the following technical scheme: the novel fed batch fermentation feeding bottle comprises a transparent bottle body, wherein a feeding component is arranged on the bottle body;
the material supplementing assembly comprises a bottle cap which is arranged on the bottle body and is in threaded connection with the bottle body;
a first silicone tube for guiding flow is arranged on one side of the top of the bottle cap, a second silicone tube for guiding flow is arranged on the other side of the top of the bottle cap, and an inner inserting tube extending to the bottom of the inner cavity of the bottle body is arranged at the bottom of the first silicone tube;
one end of the first silicone tube is provided with a three-way joint for diversion, one end of the three-way joint is provided with a third silicone tube for diversion, and the other end of the three-way joint is provided with a fourth silicone tube for diversion;
the material supplementing assembly further comprises a two-way joint which is arranged at one end of the third silicone tube and used for guiding flow, and a thermoplastic tube is arranged at one end of the two-way joint;
in one possible implementation manner, a first pipe clamp for limiting is arranged on the outer side of the first silicone pipe, a second pipe clamp for limiting is arranged on the outer side of the fourth silicone pipe, a first air filter is arranged at one end of the second silicone pipe, a second air filter is arranged at one end of the fourth silicone pipe, a third air filter is arranged at one end of the thermoplastic pipe, the first air filter, the second air filter and the third air filter are respectively fixed with the second silicone pipe, the fourth silicone pipe and the thermoplastic pipe in an inserting mode, and a sealing ring for sealing is arranged between the bottle cap and the bottle body;
according to the technical scheme, the first pipe clamp and the tail end of the thermoplastic pipe are in an open state until the pipeline at the tank opening of the fermentation tank, the second pipe clamp is in a closed state, so that shunt control is realized, the first pipe clamp is closed, the second air filter is opened, the peristaltic pump is opened again until liquid in the pipeline from the three-way joint to the tank opening of the fermentation tank completely enters the tank, the peristaltic pump is stopped, the second pipe clamp and the tail end of the thermoplastic pipe are closed until the pipe clamp on the pipeline at the tank opening of the fermentation tank is closed, the feeding is finished, the risk caused by contact of a feeding culture medium with gas generated in the fermentation tank is reduced, and the risk of leakage or pollution caused by long-time storage of the liquid in a large section of pipeline is reduced.
The utility model has the technical effects and advantages that:
compared with the prior art, the feeding assembly is simple in overall design and reasonable in structure, through corresponding matching of all structures, the first pipe clamp and the tail end of the thermoplastic pipe are in an open state until a pipeline at the tank opening of the fermentation tank, and the second pipe clamp is in a closed state, so that split flow control is realized;
closing the first pipe clamp, opening the second air filter, opening the peristaltic pump again until all liquid in the pipeline from the three-way joint to the tank opening of the fermentation tank enters the tank, stopping the peristaltic pump, closing the second pipe clamp and the pipe clamp on the pipeline from the tail end of the thermoplastic pipe to the tank opening of the fermentation tank, and ending the feeding, thereby reducing the risk caused by contact between the feeding culture medium and the gas generated in the fermentation tank and reducing the risk of leakage or pollution caused by long-time storage of the liquid in a large section of pipeline.
Drawings
In order to more clearly illustrate the technical solutions of the present disclosure, the drawings that need to be used in some embodiments of the present disclosure will be briefly described below, and it is apparent that the drawings in the following description are only drawings of some embodiments of the present disclosure, and other drawings may be obtained according to these drawings to those of ordinary skill in the art. Furthermore, the drawings in the following description may be regarded as schematic diagrams, not limiting the actual size of the products, the actual flow of the methods, the actual timing of the signals, etc. according to the embodiments of the present disclosure.
Fig. 1 is a front view of the overall structure of the present utility model.
Fig. 2 is a top view of the overall structure of the present utility model.
FIG. 3 is a cross-sectional view showing the internal structure of the bottle body according to the present utility model.
The reference numerals are: 1. a bottle body; 2. a bottle cap; 3. a first silicone tube; 4. an inner cannula; 5. a second silicone tube; 6. a three-way joint; 7. a third silicone tube; 8. a fourth silicone tube; 9. a two-way joint; 10. a thermoplastic tube; 11. a first pipe clamp; 12. a second pipe clamp; 13. a first air filter; 14. a second air filter; 15. and a third air filter.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the embodiment, as shown in the accompanying figures 1-3, the novel fed-batch fermentation feeding bottle can reduce the risk caused by contact between the feeding culture medium and the gas generated in the fermentation tank by the feeding component arranged on the bottle body 1, and reduce the risk of leakage or pollution caused by long-time storage of liquid in a large-section pipeline, and the specific structure of the component is as follows;
the material supplementing component comprises a bottle cap 2 which is arranged on the bottle body 1 and is in threaded connection with the bottle body 1;
a first silicone tube 3 for guiding flow is arranged on one side of the top of the bottle cap 2, a second silicone tube 5 for guiding flow is arranged on the other side of the top of the bottle cap 2, and an inner inserting tube 4 extending to the bottom of the inner cavity of the bottle body 1 is arranged at the bottom of the first silicone tube 3;
one end of the first silicone tube 3 is provided with a three-way joint 6 for diversion, one end of the three-way joint 6 is provided with a third silicone tube 7 for diversion, and the other end of the three-way joint 6 is provided with a fourth silicone tube 8 for diversion;
the material supplementing assembly further comprises a two-way joint 9 which is arranged at one end of the third silicone tube 7 and used for guiding flow, and a thermoplastic tube 10 is arranged at one end of the two-way joint 9;
the outside of first silicone tube 3 is provided with the first pipe clamp 11 that is used for spacing, the outside of fourth silicone tube 8 is provided with the second pipe clamp 12 that is used for spacing, the one end of second silicone tube 5 is provided with first air filter 13, the one end of fourth silicone tube 8 is provided with second air filter 14, the one end of thermoplastic tube 10 is provided with third air filter 15, first air filter 13, second air filter 14 and third air filter 15 are pegged graft fixedly with second silicone tube 5, fourth silicone tube 8 and thermoplastic tube 10 respectively, be provided with the sealing washer that is used for sealing between bottle lid 2 and the bottle 1.
When the structure is used, during the material supplementing operation, firstly, the thermoplastic pipe 10 and the thermoplastic pipe on the external fermentation tank are subjected to aseptic welding, the bottle body 1 is placed on a balance, the three-way joint 6 at the tail end of the first silicone tube 3 naturally drops and contacts with a table top, the third silicone tube 7 is clamped on an external peristaltic pump, the first pipe clamp 11 is checked, the first pipe clamp 11 and the tail end of the thermoplastic pipe 10 are ensured to be in an open state until the pipeline at the tank opening of the fermentation tank, and the second pipe clamp 12 is in a closed state;
and then starting an external peristaltic pump until the liquid level is positioned at the position of the three-way joint 6, stopping the peristaltic pump, peeling the balance, starting the peristaltic pump, stopping the peristaltic pump when the level reading reaches the preset feeding weight, closing the first pipe clamp 11, opening the second air filter 14, opening the peristaltic pump again until all liquid in the pipeline from the three-way joint 6 to the tank opening of the fermentation tank enters the tank, stopping the peristaltic pump, closing the second pipe clamp 12 and the tail end of the thermoplastic pipe 10 until the pipe clamp on the pipeline at the tank opening of the fermentation tank, and finishing feeding.
In a situation different from the prior art, the application discloses a novel fed-batch fermentation feeding bottle, be in open state through first pipe clamp 11 and thermoplastic pipe 10 end until on the pipeline of fermentation cylinder mouth department, second pipe clamp 12 is in closed state, in order to realize reposition of redundant personnel control, close first pipe clamp 11, open second air filter 14, open the peristaltic pump once more, until in the pipeline of three-way connection 6 to fermentation cylinder mouth department liquid all gets into the jar, stop the peristaltic pump, close the pipe clamp on the pipeline of second pipe clamp 12 and thermoplastic pipe 10 end until fermentation cylinder mouth department, the feeding is ended, the risk that the contact of the gas that produces in fed-batch culture medium and the fermentation cylinder has been reduced and has been brought seepage or pollution because of depositing liquid for a long time in the big section pipeline has been reduced.
The foregoing is only illustrative of the preferred embodiments of the present utility model and is not to be construed as limiting the utility model, but rather as various modifications, equivalent arrangements, improvements, etc., within the spirit and principles of the present utility model.
Claims (6)
1. A novel fed batch fermentation feeding bottle, which is characterized in that: the bottle comprises a transparent bottle body (1), wherein a material supplementing component is arranged on the bottle body (1);
the material supplementing assembly comprises a bottle cap (2) which is arranged on the bottle body (1) and is in threaded connection with the bottle body (1);
a first silicone tube (3) for guiding flow is arranged on one side of the top of the bottle cap (2), a second silicone tube (5) for guiding flow is arranged on the other side of the top of the bottle cap (2), and an inner insertion tube (4) extending to the bottom of the inner cavity of the bottle body (1) is arranged at the bottom of the first silicone tube (3);
one end of the first silicone tube (3) is provided with a three-way joint (6) used for shunting, one end of the three-way joint (6) is provided with a third silicone tube (7) used for guiding flow, and the other end of the three-way joint (6) is provided with a fourth silicone tube (8) used for guiding flow.
2. A novel fed-batch fermentation feed bottle according to claim 1, characterized in that: the material supplementing assembly further comprises a two-way joint (9) which is arranged at one end of the third silicone tube (7) and used for guiding flow, and a thermoplastic tube (10) is arranged at one end of the two-way joint (9).
3. A novel fed-batch fermentation feed bottle according to claim 1, characterized in that: the outside of first silicone tube (3) is provided with first pipe clamp (11) that are used for spacing, the outside of fourth silicone tube (8) is provided with second pipe clamp (12) that are used for spacing.
4. A novel fed-batch fermentation feed bottle according to claim 2, characterized in that: one end of the second silicone tube (5) is provided with a first air filter (13), one end of the fourth silicone tube (8) is provided with a second air filter (14), and one end of the thermoplastic tube (10) is provided with a third air filter (15).
5. A novel fed-batch fermentation feed bottle as claimed in claim 4, wherein: the first air filter (13), the second air filter (14) and the third air filter (15) are respectively inserted and fixed with the second silicone tube (5), the fourth silicone tube (8) and the thermoplastic tube (10).
6. A novel fed-batch fermentation feed bottle according to claim 1, characterized in that: a sealing ring for sealing is arranged between the bottle cap (2) and the bottle body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321749185.7U CN220745909U (en) | 2023-07-05 | 2023-07-05 | Novel fed-batch fermentation feeding bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321749185.7U CN220745909U (en) | 2023-07-05 | 2023-07-05 | Novel fed-batch fermentation feeding bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220745909U true CN220745909U (en) | 2024-04-09 |
Family
ID=90563145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321749185.7U Active CN220745909U (en) | 2023-07-05 | 2023-07-05 | Novel fed-batch fermentation feeding bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220745909U (en) |
-
2023
- 2023-07-05 CN CN202321749185.7U patent/CN220745909U/en active Active
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| GR01 | Patent grant |