CN220685160U - Colorectal cancer micro-tumor cell culture device - Google Patents
Colorectal cancer micro-tumor cell culture device Download PDFInfo
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- CN220685160U CN220685160U CN202322115099.7U CN202322115099U CN220685160U CN 220685160 U CN220685160 U CN 220685160U CN 202322115099 U CN202322115099 U CN 202322115099U CN 220685160 U CN220685160 U CN 220685160U
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- bottle
- suction pump
- cell culture
- wall
- stock solution
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- 238000004113 cell culture Methods 0.000 title claims abstract description 25
- 206010009944 Colon cancer Diseases 0.000 title claims abstract description 20
- 208000001333 Colorectal Neoplasms Diseases 0.000 title claims abstract description 20
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 49
- 239000011550 stock solution Substances 0.000 claims abstract description 28
- 239000002699 waste material Substances 0.000 claims abstract description 28
- 238000011084 recovery Methods 0.000 claims abstract description 24
- 238000003756 stirring Methods 0.000 claims description 26
- 238000011010 flushing procedure Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 230000017525 heat dissipation Effects 0.000 claims description 2
- 206010044565 Tremor Diseases 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 20
- 235000015097 nutrients Nutrition 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 238000007789 sealing Methods 0.000 description 4
- 210000004881 tumor cell Anatomy 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 1
- 208000015634 Rectal Neoplasms Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 208000030212 nutrition disease Diseases 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 206010038038 rectal cancer Diseases 0.000 description 1
- 201000001275 rectum cancer Diseases 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a colorectal cancer micro-tumor cell culture device, and relates to the technical field of cell culture. This colorectal cancer micro-tumor cell culture apparatus, the on-line screen storage device comprises a base, the top outer wall fixedly connected with incubator and operation box of base, the inner wall of operation box is provided with blake bottle, stock solution bottle, washs bottle, waste liquid recovery bottle, the inner wall grafting of stock solution bottle has the transfer line, the top of stock solution bottle is provided with the suction pump one of being connected with the transfer line, the inboard of stock solution bottle is provided with agitating unit, the one end that the stock solution bottle was kept away from to the transfer line sets up in the blake bottle, one side of wasing the bottle is provided with suction pump two. Through the cooperation of structures such as suction pump, conveniently carry out a series of operations such as waste liquid extraction, cell culture bottle wash, cell culture liquid's change, reduced artificial participation, avoided the problem of hand tremble and hand pollution, the operation is swift convenient, uses manpower sparingly material resources.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a colorectal cancer micro-tumor cell culture device.
Background
Colorectal cancer is one of a plurality of malignant tumors which seriously threaten human life and affect human body health, the recurrence and metastasis rate of colorectal cancer are high, the colorectal cancer is difficult to radically cure, patients can relapse and metastasize to different degrees within a plurality of months to a plurality of years after radical treatment, and the colorectal cancer is also an important cause of death of colorectal cancer patients. The conventional rectal cancer tumor cell culture device is characterized in that a cancer cell sample is placed in a culture solution for propagation and maintenance. After the primary cell culture is successful, the primary cell culture needs to be separated and cultured, old culture solution is poured and diluted, otherwise, the cell can influence the cell growth due to insufficient living space or overlarge density and nutrition disorder.
Through examining, chinese patent No. CN212800392U discloses a tumor cell culture apparatus, which comprises an incubator, the right side fixedly connected with motor cabinet of incubator, the last fixedly connected with first motor of motor cabinet, the output pivot fixedly connected with lead screw of first motor, the lead screw extends to the inside of incubator, the surface meshing of lead screw is connected with screw nut, screw nut's side fixedly connected with water pump, the drawing liquid pipe has been cup jointed to the bottom surface of water pump, the output fixedly connected with flexible pipe of water pump, screw nut's bottom surface fixedly connected with connecting rod, the bottom surface fixedly connected with connecting plate of connecting rod, the last fixedly connected with second motor of connecting plate, the output pivot fixedly connected with axis of rotation of second motor, the one end fixedly connected with gear of second motor is kept away from to the axis of rotation.
When the nutrient solution is absorbed through the liquid suction pipe, but when the nutrient solution is replaced, not only waste liquid is required to be extracted, but also steps such as washing and adding the nutrient solution to the culture flask are required, and when the nutrient solution is absorbed through the liquid suction pipe, other steps are required to be manually operated except that the nutrient solution is absorbed through the liquid suction pipe, so that the problem of hand pollution is easy to occur.
Disclosure of Invention
The utility model aims to provide a colorectal cancer micro-tumor cell culture device, which solves the technical problems in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a colorectal cancer microtumor cell culture apparatus, includes the base, the top outer wall fixedly connected with incubator and the operation box of base, the inner wall of operation box is provided with blake bottle, stock solution bottle, washs bottle, waste liquid recovery bottle, the inner wall grafting of stock solution bottle has the transfer line, the top of stock solution bottle is provided with the suction pump first of being connected with the transfer line, the inboard of stock solution bottle is provided with agitating unit, the one end that the stock solution bottle was kept away from to the transfer line sets up in the blake bottle, one side of washing the bottle is provided with suction pump second, the inlet end and the play water end of suction pump second are provided with wash pipe first and wash pipe second respectively, one end that the pump second was kept away from to wash pipe first and wash pipe sets up in washbottle and blake bottle respectively, one side of waste liquid recovery bottle is provided with the transfer line three, the third play water end of pump is provided with waste liquid recovery pipe first and suction bottle second respectively, the other end of waste liquid recovery bottle second suction pipe sets up in waste liquid recovery bottle, culture respectively.
Preferably, the top of blake bottle is provided with sealed lid, the inner wall top fixedly connected with electric putter of operation box, electric putter's one end is connected with sealed lid, the inner wall bottom of operation box is provided with accepts the household utensils.
Preferably, the stock solution bottle includes stock solution bottle and stock solution bottle lid, agitating unit includes agitator motor, agitator motor sets up in the outer wall of stock solution bottle lid, agitator motor's output shaft fixedly connected with puddler, the outer wall fixedly connected with stirring paddle of puddler, stirring paddle array distributes at the outer wall of puddler.
Preferably, one end of the second flushing pipe arranged in the culture bottle is fixedly connected with a flushing nozzle.
Preferably, a control box is fixedly connected to the outer wall of one side of the incubator, a host is arranged in the control box, and the first suction pump, the second suction pump, the third suction pump and the stirring motor are electrically connected with the host.
Preferably, the outer wall of the top of the incubator is uniformly provided with heat dissipation openings.
Compared with the related art, the colorectal cancer micro-tumor cell culture device provided by the utility model has the following beneficial effects:
1. through the cooperation of suction pump isotructure, conveniently carry out a series of operations such as waste liquid extraction, cell culture bottle wash, cell culture liquid's change, the function is various, and has reduced artificial participation, has avoided the problem of hand tremble and hand pollution, and the operation is swift convenient, uses manpower sparingly material resources.
2. Through agitating unit's setting, make culture solution misce bene, it is better to cultivate the effect.
3. The drip-proof liquid container has the advantages that the drip-proof container is convenient to bear drip liquid, residual waste liquid is prevented from dripping, and the experiment is clean.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic view of the structure of the operation box of the present utility model;
FIG. 3 is a schematic view of a portion of the structure of the present utility model;
fig. 4 is a schematic sectional view of a liquid storage bottle according to the present utility model.
In the figure: 1. a base; 2. an incubator; 3. an operation box; 4. a culture bottle; 5. sealing cover; 6. a liquid storage bottle; 601. a liquid storage bottle body; 602. a liquid storage bottle cap; 7. a suction pump I; 8. an infusion tube; 9. a stirring device; 901. a stirring motor; 902. a stirring rod; 903. stirring paddles; 10. cleaning the bottle; 11. a first flushing pipe; 12. a second flushing pipe; 13. a suction pump II; 14. a waste liquid recovery bottle; 15. a waste liquid recovery pipe I; 16. a waste liquid recovery pipe II; 17. a suction pump III; 18. a receiving vessel; 19. an electric push rod; 20. a control box; 21. and a heat radiation port.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model; all other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Embodiment one:
referring to fig. 1-4, the present utility model provides a technical solution: the utility model provides a colorectal cancer micro tumor cell culture apparatus, the on-line screen storage device comprises a base 1, the top outer wall fixedly connected with incubator 2 of base 1 and control box 3, the inner wall of control box 3 is provided with blake bottle 4, stock solution bottle 6, wash bottle 10, waste liquid recovery bottle 14, the inner wall grafting of stock solution bottle 6 has transfer line 8, the top of stock solution bottle 6 is provided with the suction pump 7 of being connected with transfer line 8, the inboard of stock solution bottle 6 is provided with agitating unit 9, the one end that stock solution bottle 6 was kept away from to transfer line 8 sets up in blake bottle 4, one side of wash bottle 10 is provided with suction pump two 13, the inlet end and the outlet end of suction pump two 13 are provided with wash pipe one 11 and wash pipe two 12 respectively, one end that suction pipe one 11 and wash pipe two 12 kept away from suction pump two 13 is provided with in wash bottle 10 and blake bottle 4 respectively, one side of waste liquid recovery bottle 14 is provided with suction pump three 17, suction pump three 17 water outlet end and inlet end are provided with waste liquid recovery pipe one 15 and waste liquid recovery pipe two 16 respectively, the other end of waste liquid recovery pipe two 15, waste liquid recovery pipe two 16 respectively sets up in 14, waste liquid recovery bottle 4.
Wherein, the top of blake bottle 4 is provided with sealed lid 5, and the inner wall top fixedly connected with electric putter 19 of control box 3, electric putter 19's one end is connected with sealed lid 5, and the inner wall bottom of control box 3 is provided with accepts household utensils 18.
In this embodiment, take out blake bottle 4 from incubator 2, twist the lid on the blake bottle 4, put blake bottle 4 in the container 18 that accepts in the control box 3, start electric putter 19 and drive sealed lid 5 downward movement, place sealed lid 5 in the top of blake bottle 4, after the operation is accomplished, start electric putter 19, drive sealed lid 5 upward movement, conveniently take out blake bottle 4, accept the container 18 through accepting the convenience of dropping liquid.
Wherein, one end of the second flushing pipe 12 arranged in the culture bottle 4 is fixedly connected with a flushing nozzle.
In this embodiment, the suction pump II 13 is started, and the rinse solution is supplied into the culture flask 4 through the rinse pipe I11 and the rinse pipe II 12, thereby reducing the number of cells in the culture flask 4.
Wherein, incubator 2 one side outer wall fixedly connected with control box 20 is provided with the host computer in the control box 20, and suction pump one 7, suction pump two 13, suction pump three 17, agitator motor 901 and host computer electric connection.
Wherein, the top outer wall of incubator 2 has evenly offered thermovent 21.
Embodiment two:
referring to fig. 1 to fig. 4, on the basis of the first embodiment, the present utility model provides a technical solution: wherein, stock solution bottle 6 includes stock solution bottle 601 and stock solution bottle lid 602, and agitating unit 9 includes agitator motor 901, and agitator motor 901 sets up in the outer wall of stock solution bottle lid 602, and agitator motor 901's output shaft fixedly connected with puddler 902, the outer wall fixedly connected with stirring paddle 903 of puddler 902, stirring paddle 903 array distribution is at the outer wall of puddler 902.
In this embodiment, through starting agitator motor 901, agitator motor 901 drives puddler 902 and rotates, makes stirring paddle 903 stir the culture solution, makes the culture solution misce bene, and the cultivation effect is better.
Working principle: after the primary culture of the culture flask 4 is successful, as the culture time is prolonged and the cells are continuously divided, the cells are contacted with each other to inhibit the contact, the growth speed is reduced or even stopped, and on the other hand, the growth or poisoning is not facilitated due to insufficient nutrients and accumulation of metabolites, so that the old culture solution in the culture flask 4 needs to be washed or poured out; taking the culture bottle 4 out of the incubator 2, screwing off a cover on the culture bottle 4, placing the culture bottle 4 in a receiving vessel 18 in the operation box 3, starting an electric push rod 19 to drive the sealing cover 5 to move downwards, and placing the sealing cover 5 above the culture bottle 4; starting a suction pump III 17, pumping out the culture solution in the culture bottle 4, starting a suction pump II 13, conveying the flushing solution into the culture bottle 4 through a flushing pipe I11 and a flushing pipe II 12, pumping out the flushing solution in the culture bottle 4 through the suction pump III 17, adding the culture solution into the liquid storage bottle 6 through a suction pump I7 after the operation is finished, starting a stirring motor 901, driving a stirring rod 902 to rotate by the stirring motor 901, stirring the culture solution by a stirring blade 903 to be uniformly mixed, and starting the stirring motor I7 to add the liquid into the culture bottle 4 through a liquid conveying pipe 8; after the operation is finished, the electric push rod 19 is started to drive the sealing cover 5 to move upwards, so that the culture bottle 4 is conveniently taken out, the dripped liquid is conveniently received through the receiving vessel 18, residual waste liquid is prevented from dripping, and the experiment is cleaned.
Claims (6)
1. Colorectal cancer micro-tumor cell culture device, including base (1), its characterized in that: the utility model discloses a waste liquid recovery device, including base (1), incubator (2) and control box (3) of top outer wall fixedly connected with of base (1), the inner wall of control box (3) is provided with blake bottle (4), stock solution bottle (6), washout bottle (10), waste liquid recovery bottle (14), the inner wall of stock solution bottle (6) is pegged graft and is had transfer line (8), the top of stock solution bottle (6) is provided with suction pump (7) that are connected with transfer line (8), the inboard of stock solution bottle (6) is provided with agitating unit (9), the one end that stock solution bottle (6) was kept away from to transfer line (8) sets up in blake bottle (4), one side of washout bottle (10) is provided with suction pump two (13), the water inlet end and the water outlet end of suction pump two (13) are provided with flushing pipe two (11) and flushing pipe two (12) respectively, the one end that suction bottle two (11) and flushing pipe two (12) keep away from suction pump two (13) are provided with in suction pump (10) and culture (4) respectively, one side (14) is provided with three suction pump (17) and one waste liquid recovery end (15) are provided with waste liquid recovery pump two (15), one side (17) is provided with waste liquid recovery end and one recovery end (15) The other end of the waste liquid recovery pipe II (16) is respectively arranged in the waste liquid recovery bottle (14) and the culture bottle (4).
2. The colorectal cancer micro-tumor cell culture apparatus according to claim 1, wherein: the top of blake bottle (4) is provided with sealed lid (5), the inner wall top fixedly connected with electric putter (19) of control box (3), the one end and the sealed lid (5) of electric putter (19) are connected, the inner wall bottom of control box (3) is provided with accepts household utensils (18).
3. The colorectal cancer micro-tumor cell culture apparatus according to claim 1, wherein: the liquid storage bottle (6) comprises a liquid storage bottle body (601) and a liquid storage bottle cap (602), the stirring device (9) comprises a stirring motor (901), the stirring motor (901) is arranged on the outer wall of the liquid storage bottle cap (602), an output shaft of the stirring motor (901) is fixedly connected with a stirring rod (902), stirring paddles (903) are fixedly connected to the outer wall of the stirring rod (902), and the stirring paddles (903) are distributed on the outer wall of the stirring rod (902) in an array mode.
4. The colorectal cancer micro-tumor cell culture apparatus according to claim 1, wherein: one end of the second flushing pipe (12) arranged in the culture bottle (4) is fixedly connected with a flushing nozzle.
5. A colorectal cancer micro-tumor cell culture apparatus according to claim 3, wherein: one side outer wall fixedly connected with control box (20) of incubator (2), be provided with the host computer in control box (20), suction pump one (7), suction pump two (13), suction pump three (17), agitator motor (901) and host computer electric connection.
6. The colorectal cancer micro-tumor cell culture apparatus according to claim 1, wherein: and heat dissipation openings (21) are uniformly formed in the outer wall of the top of the incubator (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322115099.7U CN220685160U (en) | 2023-08-07 | 2023-08-07 | Colorectal cancer micro-tumor cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322115099.7U CN220685160U (en) | 2023-08-07 | 2023-08-07 | Colorectal cancer micro-tumor cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220685160U true CN220685160U (en) | 2024-03-29 |
Family
ID=90378367
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322115099.7U Active CN220685160U (en) | 2023-08-07 | 2023-08-07 | Colorectal cancer micro-tumor cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220685160U (en) |
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2023
- 2023-08-07 CN CN202322115099.7U patent/CN220685160U/en active Active
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