CN220685099U - Stirring device for nucleic acid extraction - Google Patents

Abstract

The utility model discloses a stirring device for nucleic acid extraction, which belongs to the technical field of gene detection and comprises a supporting table, a movable groove, a movable assembly and a test tube seat, wherein the movable groove is formed in the upper surface of the supporting table, the movable assembly is movably arranged in the movable groove, the test tube seat is movably arranged on the upper surface of the movable assembly, the test tube seat is formed with a test tube groove, the upper surface of the supporting table is fixedly connected with the stirring assembly, the side surface of the stirring assembly is fixedly connected with a tube sleeve frame, and the upper surface of the stirring assembly is fixedly connected with a lifting assembly. The clamping groove on the card seat plate is convenient for people to move to the position convenient for replacing the test tube seat, so that people can integrally replace the test tube seat filled with the new test tube reagent, and the test tube reagent is convenient for people to replace.

Description

Stirring device for nucleic acid extraction

Technical Field

The utility model relates to the technical field of gene detection, in particular to a stirring device for nucleic acid extraction.

Background

The gene detection generally comprises the processes of sample collection, nucleic acid extraction, reagent preparation, amplification, on-machine detection and the like, wherein the purpose of nucleic acid extraction is to decompose proteins in a sample so as to expose the nucleic acid to an extracting solution, thereby facilitating the PCR amplification reaction; the use of high throughput, automated nucleic acid extractors in nucleic acid extraction processes has become increasingly popular, greatly improving the efficiency of nucleic acid extraction and reducing the workload of operators. When carrying out nucleic acid extraction operation, need to change test tube reagent many times according to standard flow to and sample and reagent intensive mixing just can carry out nucleic acid extraction, and current relevant equipment is comparatively inconvenient when changing test tube reagent, and stirring mixing efficiency is lower, in order to solve above-mentioned problem, we have proposed following device.

Disclosure of Invention

(1) Technical problem to be solved

Aiming at the defects of the prior art, the utility model aims to provide a stirring device for nucleic acid extraction, which has the characteristics of convenient replacement of test tube reagents and higher stirring and mixing efficiency of sample reagents.

(2) Technical proposal

In order to achieve the above object, the utility model provides a stirring device for nucleic acid extraction, which comprises a supporting table, a movable groove, a movable assembly and a test tube seat, wherein the movable groove is formed in the upper surface of the supporting table, the movable assembly is movably arranged in the movable groove, the test tube seat is movably arranged on the upper surface of the movable assembly, the test tube seat is formed in the test tube seat, the stirring assembly is fixedly connected with the upper surface of the supporting table, the side surface of the stirring assembly is fixedly connected with a pipe sleeve frame, the upper surface of the stirring assembly is fixedly connected with a lifting assembly, the side surface of the lifting assembly is fixedly connected with a magnetic rod frame, the magnetic rod frame is provided with a magnetic rod substrate through a matching groove, the lower surface of the magnetic rod substrate is fixedly connected with a magnetic rod, a first T-shaped groove is formed in the side surface of the pipe sleeve frame, the long through hole is communicated with the first T-shaped magnetic sleeve substrate is clamped in the first T-shaped groove, and the magnetic rod sleeve is penetrated on the upper surface of the T-shaped magnetic sleeve substrate.

When the stirring device for nucleic acid extraction is used, through the arrangement of the handle, the T-shaped guide rail and the second T-shaped groove, when people need to replace test tubes, firstly, people only need to pull the handle in the state that the magnetic rod sleeve is separated from the test tubes for testing, and under the cooperation of the T-shaped guide rail and the second T-shaped groove. The clamping groove on the card seat plate is convenient for people to move to the position convenient for replacing the test tube seat, so that people can integrally replace the test tube seat filled with the new test tube reagent, and the test tube reagent is convenient for people to replace.

Further, the movable assembly comprises a movable substrate, the movable substrate is movably arranged in a movable groove, a T-shaped guide rail is fixedly connected to the side surface of the movable groove, a second T-shaped groove is formed in the side surface of the movable groove, the T-shaped guide rail is arranged in the second T-shaped groove in a penetrating mode, the T-shaped guide rail and the second T-shaped groove are two groups in total and are symmetrically arranged on the side surface of the movable substrate, a clamping seat plate is fixedly connected to the upper surface of the movable substrate, a clamping groove is formed in the upper surface of the clamping seat plate, and the test tube seat is movably arranged in the clamping groove.

Further, the magnetic bars and the test tube grooves are equally arranged in a rectangular mode, the positions are in one-to-one correspondence, the long through holes, the first T-shaped grooves and the T-shaped magnetic sleeve base plates are shared in multiple groups, the number of the long through holes is equal to the number of the magnetic bars, the positions are in one-to-one correspondence, the magnetic bars are shared in multiple groups, the number of the magnetic bars is equal to the number of the magnetic bars, and the positions are in one-to-one correspondence.

Further, the stirring assembly comprises a stirring table, stirring table fixed connection is at the supporting bench upper surface, first transmission storehouse has been seted up to stirring table inside, fixedly connected with riser in the first transmission storehouse, first bearing is worn to be equipped with in the riser side, wear to be equipped with first pivot in the first bearing, fixedly connected with first motor in the first transmission storehouse, first motor output shaft fixed connection is in the one end of first pivot, first pivot other end fixedly connected with carousel, the cross recess has been seted up to the stirring table side, the activity is provided with the cross movable block in the cross recess, cross movable block side fixedly connected with first connecting block, first connecting block side fixedly connected with diaphragm, the bearing groove has been seted up to the diaphragm side, the activity is provided with antifriction bearing in the bearing groove, carousel side fixedly connected with second pivot, the second pivot other end wears to be established in antifriction bearing, magnetic bar frame fixed connection is in the cross movable block side.

Further, the lifting assembly comprises a lifting table, the lifting table is fixedly connected to the upper surface of the stirring table, a second transmission bin is formed in the lifting table, a second motor is fixedly connected to the second transmission bin, a threaded column is fixedly connected to an output shaft of the second motor, a third rotating shaft is fixedly connected to the other end of the threaded column, a third bearing is fixedly connected to the second transmission bin, the other end of the third bearing is arranged in the third bearing in a penetrating mode, a threaded pipe is connected to the surface of the threaded column in a threaded mode, a limiting groove is formed in the side face of the lifting table, a second connecting block is movably arranged in the limiting groove, one end of the second connecting block is fixedly connected to the side face of the threaded pipe, and the magnetic rod frame is fixedly connected to the other end of the second connecting block.

Further, an energy storage bin is arranged in the supporting table, and a storage battery is fixedly connected in the energy storage bin.

(3) Advantageous effects

In summary, the utility model has the following beneficial effects:

1. this agitating unit for nucleic acid extraction through setting up handle, T shape guide rail and second T shape groove, when people need carry out the test tube and change, at first people only need pull the handle under the state that the bar magnet cover breaks away from the test tube test, under the cooperation in T shape guide rail and second T shape groove. The clamping groove on the clamping seat plate is convenient for people to move to the position convenient for replacing the test tube seat, so that people can integrally replace the test tube seat filled with a new test tube reagent, and the test tube reagent is convenient for people to replace;

2. this agitating unit for nucleic acid extraction, through setting up first motor, the second pivot, antifriction bearing and bearing groove, when people need carry out sample reagent stirring and mix, people at first drive first motor operation through display controller, thereby drive first pivot and carousel high-speed rotation, thereby drive second pivot and antifriction bearing and do high-speed circular motion, under antifriction bearing and bearing groove's cooperation, first linkage piece is high-speed reciprocating motion, thereby drive the cross movable block and do high-speed reciprocating motion in the cross groove, and then make the bar magnet cover on the pipe box frame do high-speed reciprocating motion in the test tube that corresponds, and then improved the stirring efficiency of sample and reagent.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings required for the description of the embodiments or the prior art will be briefly described, and it is apparent that the drawings in the following description are only one embodiment of the present utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a front perspective view of the present utility model;

FIG. 2 is a schematic diagram of a front perspective cross-sectional structure of a mobile assembly of the present utility model;

FIG. 3 is a schematic elevational view in perspective and cross-section of the stirring assembly of the present utility model;

FIG. 4 is a schematic view of an exploded view of the stirring assembly of the present utility model;

FIG. 5 is a schematic elevational view in perspective and cross-sectional configuration of the lift assembly of the present utility model;

fig. 6 is a schematic structural view of a pipe sleeve frame and a part of a connecting piece thereof according to the present utility model.

The marks in the drawings are:

1. a support table; 2. a movable groove; 3. a moving assembly; 4. a test tube holder; 5. a test tube groove; 6. a stirring assembly; 7. a pipe sleeve frame; 8. a lifting assembly; 9. a magnetic bar frame; 10. a magnetic bar substrate; 11. a magnetic bar; 12. a long through hole; 13. a first T-shaped slot; 14. a T-shaped magnetic sleeve substrate; 15. a magnetic rod sleeve; 16. an energy storage bin; 17. a storage battery; 18. a display controller; 301. moving the substrate; 302. a T-shaped guide rail; 303. a second T-shaped slot; 304. a card seat board; 305. a clamping groove; 306. a handle; 601. a stirring table; 602. a first drive bin; 603. a riser; 604. a first bearing; 605. a first rotating shaft; 606. a first motor; 607. a turntable; 608. a cross groove; 609. a cross moving block; 610. a first connection block; 611. a cross plate; 612. a bearing groove; 613. a second rotating shaft; 614. a rolling bearing; 801. a lifting table; 802. a second transmission bin; 803. a second motor; 804. a threaded column; 805. a third rotating shaft; 806. a third bearing; 807. a threaded tube; 808. a limit groove; 809. and a second connecting block.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the present utility model easy to understand, the technical solutions in the embodiments of the present utility model are clearly and completely described below to further illustrate the present utility model, and it is obvious that the described embodiments are only some embodiments of the present utility model, not all versions.

Examples:

the utility model is described in further detail below with reference to fig. 1-6.

Referring to fig. 1-6, the present utility model provides a technical solution: the utility model provides a stirring device for nucleic acid extraction, including supporting bench 1, movable groove 2, remove subassembly 3 and test tube seat 4, movable groove 2 sets up at supporting bench 1 upper surface, the activity of movable subassembly 3 sets up in movable groove 2, test tube seat 4 activity sets up at removal subassembly 3 upper surface, test tube groove 5 has been seted up on the test tube seat 4, fixed surface is connected with stirring subassembly 6 on supporting bench 1, stirring subassembly 6 side fixedly connected with pipe box frame 7, fixed surface is connected with lifting unit 8 on stirring subassembly 6, lifting unit 8 side fixedly connected with bar magnet frame 9, bar magnet frame 9 is provided with bar magnet base plate 10 through the cooperation groove, bar magnet base plate 10 lower surface fixedly connected with magnetic bar 11, first T-shaped groove 13 has been seted up to pipe box frame 7 side, long through-hole 12 has been seted up on pipe box frame 7 surface, and long through-hole 12 and first T-shaped groove 13 intercommunication, the card is equipped with T-shaped magnetic sleeve base plate 14 in the first T-shaped magnetic sleeve base plate 13, the bar magnet sleeve 15 is worn to be equipped with on T-shaped magnetic sleeve base plate 14 upper surface.

Through adopting above-mentioned technical scheme, this agitating unit for nucleic acid extraction through setting up handle 306, T shape guide rail 302 and second T-shaped groove 303, when people need carry out the test tube and change, at first people only need pull handle 306 under the state that bar magnet cover 15 breaks away from the test tube test, under the cooperation of T shape guide rail 302 and second T-shaped groove 303. The clamping groove 305 on the card seat plate 304 is convenient for people to move to the position convenient for replacing the test tube seat 4, so that people can integrally replace the test tube seat 4 filled with new test tube reagent, and the test tube reagent is convenient for people to replace.

Specifically, the moving assembly 3 includes a moving substrate 301, the moving substrate 301 is movably disposed in the moving groove 2, a T-shaped guide rail 302 is fixedly connected to a side surface of the moving substrate 301, a second T-shaped groove 303 is formed in a side surface of the moving groove 2, the T-shaped guide rail 302 is penetratingly disposed in the second T-shaped groove 303, two groups of T-shaped guide rails 302 and the second T-shaped groove 303 are symmetrically disposed on a side surface of the moving substrate 301, a card seat board 304 is fixedly connected to an upper surface of the moving substrate 301, a card slot 305 is formed in an upper surface of the card seat board 304, and a test tube seat 4 is movably disposed in the card slot 305.

Through adopting above-mentioned technical scheme, this agitating unit for nucleic acid extraction, through setting up first motor 606, second pivot 613, antifriction bearing 614 and bearing groove 612, when people need carry out sample reagent stirring mix, people at first drive first motor 606 operation through display controller 18, thereby drive first pivot 605 and carousel 607 high-speed rotation, thereby drive second pivot 613 and antifriction bearing 614 and do high-speed circular motion, under antifriction bearing 614 and bearing groove 612's cooperation, first linkage piece 610 is high-speed reciprocating motion, thereby drive cross movable piece 609 and do high-speed reciprocating motion in cross groove 608, and then make the bar magnet cover 15 on the pipe box frame 7 do high-speed reciprocating motion in the test tube that corresponds, and then improved sample and reagent's stirring efficiency.

Specifically, the number of the magnetic bars 11 and the number of the test tube grooves 5 are equal and are in rectangular arrangement, the positions are in one-to-one correspondence, the number of the long through holes 12, the first T-shaped grooves 13 and the T-shaped magnetic sleeve base plates 14 are in multiple groups, the number of the long through holes is equal to the number of columns of the magnetic bars 11, the positions are in one-to-one correspondence, the number of the magnetic bar sleeves 15 is in multiple groups, the number of the magnetic bar sleeves is equal to the number of the rows of the magnetic bars 11, and the positions are in one-to-one correspondence.

Specifically, stirring subassembly 6 includes stirring platform 601, stirring platform 601 fixed connection is at supporting bench 1 upper surface, first transmission storehouse 602 has been seted up to stirring platform 601 inside, fixedly connected with riser 603 in the first transmission storehouse 602, first bearing 604 is worn to be equipped with in the riser 603 side, wear to be equipped with first pivot 605 in the first bearing 604, fixedly connected with first motor 606 in the first transmission storehouse 602, first motor 606 output shaft fixed connection is in the one end of first pivot 605, first pivot 605 other end fixedly connected with carousel 607, cross groove 608 has been seted up to stirring platform 601 side, the activity is provided with cross movable block 609 in the cross groove 608, cross movable block 609 side fixedly connected with first connecting block 610, first connecting block 610 side fixedly connected with diaphragm 611 has been seted up to the diaphragm 611 side, movably provided with antifriction bearing 614 in the bearing 612, carousel 607 side fixedly connected with second pivot 613, the second pivot 613 other end is worn to be equipped with in antifriction bearing 614, bar 9 fixed connection is in cross movable block 609 side.

Specifically, lifting component 8 includes elevating platform 801, elevating platform 801 fixed connection is at the stirring platform 601 upper surface, second transmission storehouse 802 has been seted up to elevating platform 801 inside, fixedly connected with second motor 803 in the second transmission storehouse 802, fixedly connected with screw post 804 on the second motor 803 output shaft, the screw post 804 other end fixedly connected with third pivot 805, fixedly connected with third bearing 806 in the second transmission storehouse 802, the third bearing 806 other end wears to establish in third bearing 806, screw post 804 surface threaded connection has screwed pipe 807, spacing groove 808 has been seted up to elevating platform 801 side, the activity is provided with second connecting block 809 in the spacing groove 808, second connecting block 809 one end fixed connection is in screwed pipe 807 side, bar magnet frame 9 fixed connection is at the second connecting block 809 other end.

Specifically, an energy storage bin 16 is arranged in the supporting table 1, and a storage battery 17 is fixedly connected in the energy storage bin 16.

The working principle of the utility model is as follows: in use, one would need to work in conjunction with standard nucleic acid extraction procedures, such as the following,

firstly, a plurality of samples required to be subjected to nucleic acid detection are respectively mixed with magnetic beads and an extraction reagent and added into different test tubes, and then the test tubes with the samples are orderly placed into different test tube grooves 5 in a same-column priority mode;

step two, orderly inserting the needed T-shaped magnetic sleeve substrate 14 into the first T-shaped groove 13;

third, the first motor 606 is driven to operate by the display controller 18, so that the first rotating shaft 605 is driven to rotate at a high speed, the turntable 607 is driven to rotate at a high speed, the second rotating shaft 613 and the rolling bearing 614 are driven to do high-speed circular motion, the first connecting block 610 is driven to do high-speed reciprocating motion under the cooperation of the rolling bearing 614 and the bearing groove 612, the cross moving block 609 is driven to do high-speed reciprocating motion in the cross groove 608, and the magnetic rod sleeve 15 on the pipe sleeve frame 7 is driven to do high-speed reciprocating motion in a corresponding test tube, so that samples, magnetic beads and extraction reagents are stirred;

fourth, the second motor 803 is controlled to operate by the display controller 18, the second motor 803 drives the threaded column 804 to rotate, the threaded column 804 is matched with the threaded tube 807, the threaded tube 807 drives the second connecting block 809 to move downwards, and meanwhile drives the magnetic rod frame 9, the magnetic rod substrate 10 and the magnetic rod 11 to move downwards until the magnetic rod 11 completely enters the magnetic rod sleeve 15, and the magnetic rod 11 adsorbs magnetic beads on the surface of the magnetic rod sleeve 15;

fifth, pull the handle 306 in the state that the magnetic rod tube sleeve breaks away from the test tube, under the cooperation of the T-shaped guide rail 302 and the second T-shaped groove 303, one can move the clamping groove 305 on the clamping seat plate 304 to a position convenient for replacing the test tube seat 4, then one can put the test tube seat 4 filled with new test tube reagent into the clamping groove 305, and then push the handle 306 to reset the clamping seat plate 304, so as to replace the new test tube reagent;

step six, driving the lifting assembly 8 again through the display controller 18, so that the magnetic rod 11 is separated from the magnetic rod sleeve 15, and the magnetic beads fall into a new test tube reagent;

the subsequent operations such as magnetic bead recovery can be performed by referring to the above-described procedure in combination with conventional test steps for nucleic acid extraction, and will not be described here.

The present embodiment is only for explanation of the present utility model and is not to be construed as limiting the present utility model, and modifications to the present embodiment, which may not creatively contribute to the present utility model as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present utility model.

Claims (6)

1. The utility model provides a stirring device for nucleic acid extraction, includes brace table (1), movable groove (2), remove subassembly (3) and test tube seat (4), its characterized in that: the movable groove (2) is formed in the upper surface of the supporting table (1), the movable assembly (3) is movably arranged in the movable groove (2), the test tube seat (4) is movably arranged on the upper surface of the movable assembly (3), the test tube seat (4) is provided with the test tube groove (5), the upper surface of the supporting table (1) is fixedly connected with the stirring assembly (6), the side surface of the stirring assembly (6) is fixedly connected with the pipe sleeve frame (7), the upper surface of the stirring assembly (6) is fixedly connected with the lifting assembly (8), the side surface of the lifting assembly (8) is fixedly connected with the magnetic rod frame (9), the magnetic rod rack (9) is provided with a magnetic rod substrate (10) through a matching groove, a magnetic rod (11) is fixedly connected to the lower surface of the magnetic rod substrate (10), a first T-shaped groove (13) is formed in the side face of the pipe sleeve rack (7), a long through hole (12) is formed in the surface of the pipe sleeve rack (7), the long through hole (12) is communicated with the first T-shaped groove (13), a T-shaped magnetic sleeve substrate (14) is clamped in the first T-shaped groove (13), and a magnetic rod sleeve (15) is arranged on the upper surface of the T-shaped magnetic sleeve substrate (14) in a penetrating mode.

2. The stirring device for nucleic acid extraction according to claim 1, wherein: the mobile assembly (3) comprises a mobile substrate (301), the mobile substrate (301) is movably arranged in a movable groove (2), a T-shaped guide rail (302) is fixedly connected to the side surface of the mobile substrate (301), a second T-shaped groove (303) is formed in the side surface of the movable groove (2), the T-shaped guide rail (302) is arranged in the second T-shaped groove (303) in a penetrating mode, the T-shaped guide rail (302) and the second T-shaped groove (303) are shared in two groups and are symmetrically arranged on the side surface of the mobile substrate (301), a clamping seat plate (304) is fixedly connected to the upper surface of the mobile substrate (301), a clamping groove (305) is formed in the upper surface of the clamping seat plate (304), and a test tube seat (4) is movably arranged in the clamping groove (305).

3. The stirring device for nucleic acid extraction according to claim 1, wherein: the magnetic rod (11) and the test tube groove (5) are equal in number and are in rectangular arrangement, the positions are in one-to-one correspondence, the long through holes (12), the first T-shaped grooves (13) and the T-shaped magnetic sleeve base plates (14) are shared in multiple groups, the number of the long through holes is equal to the number of columns of the magnetic rod (11), the magnetic rod sleeves (15) are in one-to-one correspondence, the magnetic rod sleeves (15) are shared in multiple groups, the number of the magnetic rod sleeves is equal to the number of the rows of the magnetic rod (11), and the positions are in one-to-one correspondence.

4. The stirring device for nucleic acid extraction according to claim 1, wherein: the stirring assembly (6) comprises a stirring table (601), the stirring table (601) is fixedly connected to the upper surface of the supporting table (1), a first transmission bin (602) is formed in the stirring table (601), a vertical plate (603) is fixedly connected to the first transmission bin (602), a first bearing (604) is arranged on the side face of the vertical plate (603) in a penetrating mode, a first rotating shaft (605) is arranged on the first bearing (604) in a penetrating mode, a first motor (606) is fixedly connected to the first transmission bin (602), an output shaft of the first motor (606) is fixedly connected to one end of the first rotating shaft (605), a rotary table (607) is fixedly connected to the other end of the first rotating shaft (605), a cross groove (608) is formed in the side face of the stirring table (601), a cross moving block (609) is movably arranged in the cross groove (608), a first connecting block (610) is fixedly connected to the side face of the first connecting block (610), a transverse plate (611) is fixedly connected to the side face of the first connecting block (607), a rotary table (612) is fixedly connected to the second rotating shaft (613) in the side face of the first rotating shaft (613), the magnetic bar frame (9) is fixedly connected to the side face of the cross moving block (609).

5. The stirring device for nucleic acid extraction according to claim 1, wherein: lifting component (8) are including elevating platform (801), elevating platform (801) fixed connection is in stirring platform (601) upper surface, second transmission storehouse (802) have been seted up to elevating platform (801) inside, fixedly connected with second motor (803) in second transmission storehouse (802), fixedly connected with screw thread post (804) on second motor (803) output shaft, screw thread post (804) other end fixedly connected with third pivot (805), fixedly connected with third bearing (806) in second transmission storehouse (802), third bearing (806) other end wears to establish in third bearing (806), screw thread post (804) surface threaded connection has screwed pipe (807), limiting groove (808) have been seted up to elevating platform (801) side, activity is provided with second connecting block (809) in limiting groove (808), second connecting block (809) one end fixed connection is in screwed pipe (807) side, magnetic rod frame (9) fixed connection is in second connecting block (809) other end.

6. The stirring device for nucleic acid extraction according to claim 1, wherein: an energy storage bin (16) is arranged in the supporting table (1), and a storage battery (17) is fixedly connected in the energy storage bin (16).