CN220626214U

CN220626214U - Exfoliated cell finding instrument

Info

Publication number: CN220626214U
Application number: CN202322236744.0U
Authority: CN
Inventors: 高海波; 杨玉平
Original assignee: Beijing Ocean Ht Technology Co ltd
Current assignee: Beijing Ocean Ht Technology Co ltd
Priority date: 2023-08-21
Filing date: 2023-08-21
Publication date: 2024-03-19
Anticipated expiration: 2033-08-21

Abstract

The utility model discloses an exfoliated cell discovery instrument, which comprises a host platform, a detection device and a control device, wherein the host platform is provided with a triaxial adjusting platform and an imaging assembly for detecting a detection material positioned on the triaxial adjusting platform; computer and HMI operation desk; the imaging assembly is integrated on the Z-axis electric translation table, and the imaging assembly adjusts the position relative to the inspected material through the Z-axis electric translation table; imaging data of the exfoliated cells in the examination material collected by the imaging component is transmitted to the computer and displayed by a display of the computer. The exfoliated cell discovery instrument can directly discover exfoliated cells on various detection materials, so that DNA genetic materials can be directly discovered and extracted, and pollution caused by dyeing is avoided; the method does not need to carry out various pre-chemical treatments on the detection material, does not influence other technical detection, and belongs to a nondestructive detection method.

Description

Exfoliated cell finding instrument

Technical Field

The utility model relates to the technical field of DNA physical evidence inspection, in particular to an exfoliated cell discovery instrument which is suitable for discovering exfoliated cells in DNA physical evidence inspection in all forensic science, and further confirms the technology of effectively discovering and extracting DNA.

Background

In the current forensic science, DNA is the most direct and effective technical means for testing and identifying suspects, but the current methods for finding DNA are indirect methods, such as the most commonly used techniques using optical methods or using amino acid visualization reagents, and all are indirect methods for determining the possible presence of exfoliated cells by finding amino acids in sweat; however, in reality, amino acids are present in sweat, but there are not necessarily exfoliated cells, because DNA genetic material is present in cells, not sweat, there are not necessarily exfoliated cells in sweat, and therefore, this indirect method cannot directly confirm the presence of DNA, and in reality, the extraction rate of DNA is limited.

In addition, blind extraction is easy to cause mixed extraction, and DNA typing after mixing is extremely difficult, so that no effective method capable of directly extracting exists in the prior art.

Therefore, based on the above technical problems, a person skilled in the art is highly required to develop an exfoliated cell discovery apparatus.

Disclosure of Invention

The utility model aims to provide an exfoliated cell discovery instrument which can directly discover exfoliated cells and assist in the subsequent extraction of DNA in the exfoliated cells.

In order to achieve the above object, the present utility model provides the following technical solutions:

an exfoliated cell finding instrument of the present utility model includes:

the main machine platform is provided with a three-axis adjusting platform and an imaging assembly for checking the checking material positioned on the three-axis adjusting platform;

a computer connected to the imaging assembly; and

an HMI console for controlling the host platform;

the triaxial adjustment platform is divided into:

an X-axis electric translation stage, a Y-axis electric translation stage and a Z-axis electric translation stage;

the X-axis electric translation stage is positioned on the Y-axis electric translation stage, the detection material is placed on the Y-axis electric translation stage, and the detection material adjusts the positions of the X direction and the Y direction through the X-axis electric translation stage and the Y-axis electric translation stage so as to adjust the position relative to the Z-axis electric translation stage;

the imaging assembly is integrated with the Z-axis electric translation stage, and the imaging assembly adjusts the position relative to the inspected material through the Z-axis electric translation stage;

imaging data of the exfoliated cells in the examination material collected by the imaging component are transmitted to the computer and displayed by a display of the computer.

Further, a sliding seat is connected to the Z-axis electric translation table in a sliding manner, and a support arm is arranged on the sliding seat;

the imaging component is arranged at the end part of the support arm far away from one end of the Z-axis electric translation stage.

Further, the imaging assembly includes:

an imaging assembly housing, the upper portion of which is integrated with a display screen;

the zoom lens barrel is integrated in the imaging assembly shell and extends along the axial direction of the imaging assembly shell, an imaging objective lens is arranged at the lower end of the zoom lens barrel, and a high-speed camera is arranged at the upper end of the zoom lens barrel;

the lower end of the imaging component shell is provided with a multiband light source system;

the imaging component shell is internally integrated with a magnification adjusting mechanism, and the magnification adjusting mechanism is connected with the zoom lens barrel and controls the zoom lens barrel.

Further, a control circuit board is integrated inside the imaging component shell.

Further, a double red dot positioning structure is integrated on the side face of the imaging component shell;

the dual red dot positioning structure emits two red mark dots toward the surface of the Y-axis electric translation stage.

Further, the HMI console includes:

the operation desk comprises an operation desk shell, wherein the bottom of the operation desk shell is provided with a bottom plate, and a light source control screen is arranged at the upper part of the operation desk shell and used for controlling the multiband light source system;

one side of the operating platform shell is provided with a three-dimensional control handle which is used for respectively controlling the actions of the X-axis electric translation platform, the Y-axis electric translation platform and the Z-axis electric translation platform;

the operation desk shell is also provided with an emergency stop switch and a key switch.

Further, an exposure adjusting knob and a light source intensity adjusting knob are arranged on the HMI operation table.

In the technical scheme, the exfoliated cell discovery instrument provided by the utility model has the following beneficial effects:

the exfoliated cell discovery instrument can directly discover exfoliated cells on various detection materials, so that DNA genetic materials can be directly discovered and extracted, and pollution caused by dyeing is avoided; the method does not need to carry out various pre-chemical treatments on the detection material, does not influence other technical detection, and belongs to a nondestructive detection method.

The exfoliated cell discovery instrument can perform point-to-point targeted extraction, and avoids the incapability of parting after mixed extraction caused by blind extraction.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present utility model, and other drawings may be obtained according to these drawings for a person having ordinary skill in the art.

FIG. 1 is a graph showing the comparison of the detection effect of conventional fluorescence detection in the prior art;

FIG. 2 is a graph showing the detection effect of the exfoliated cell finder disclosed in this example (cotton velvet material on the left side; tissue debris with biological characteristics on the right side);

FIG. 3 is a schematic diagram showing the split extraction after positioning in the exfoliated cell finder disclosed in this example;

FIG. 4 is a graph showing the effect of the exfoliated cell finder disclosed in the present embodiment on detecting different materials by using different light sources;

FIG. 5 is a schematic diagram showing the structure of an exfoliated cell finder disclosed in this example;

FIG. 6 is an exploded view of the host platform of the exfoliated cell finder disclosed in this embodiment;

FIG. 7 is an exploded view of the HMI console of the exfoliated cell finder disclosed in this embodiment;

FIG. 8 is a schematic diagram of a multi-band light source system of an exfoliated cell finder disclosed in this embodiment;

FIG. 9 is a schematic diagram showing the structure of the double red spot positioning structure of the exfoliated cell finder disclosed in this embodiment.

Reference numerals illustrate:

1. a host platform; 2. a computer; 3. an HMI console;

101. an X-axis electric translation stage; 102. y-axis electric translation stage; 103. a Z-axis electric translation stage; 104. a support arm; 105. an imaging assembly housing; 106. a detector; 107. a zoom lens barrel; 108. an imaging objective; 109. a high-speed camera; 110. a magnification adjusting mechanism; 111. a control circuit board; 112. a multi-band light source system; 113. a double red dot positioning structure;

11201. a light source housing; 11202. a light source base; 11203. 365nm ultraviolet light source; 11204. an infrared source of 850 nm; 11205. a first 405 violet light source; 11206. a 525 blue light source; 11207. 445nm green light source; 11208. a second 405nm violet light source; 11209. left white light; 11210. right white light;

11301. a first red dot; 11302. a second red dot;

301. an operation panel housing; 302. a three-dimensional control handle; 303. a bottom plate; 304. an emergency stop switch; 305. a light source control screen; 306. a key switch; 307. an exposure adjustment knob; 308. a light source intensity adjusting knob.

Detailed Description

In order to make the technical scheme of the present utility model better understood by those skilled in the art, the present utility model will be further described in detail with reference to the accompanying drawings.

See fig. 1-9;

the embodiment discloses a exfoliated cell discovery instrument, the discovery instrument includes:

the device comprises a host platform 1, wherein the host platform 1 is provided with a triaxial adjusting platform and an imaging assembly for checking a checking material positioned on the triaxial adjusting platform;

a computer 2 connected to the imaging assembly; and

an HMI console 3 for controlling the host platform 1;

the triaxial adjustment platform is divided into:

an X-axis motorized translation stage 101, a Y-axis motorized translation stage 102, and a Z-axis motorized translation stage 103;

the X-axis electric translation stage 101 is positioned on the Y-axis electric translation stage 102, and a material to be inspected is placed on the Y-axis electric translation stage 102, and the position of the material to be inspected in the X direction and the Y direction is adjusted through the X-axis electric translation stage 101 and the Y-axis electric translation stage 102 so as to adjust the position relative to the Z-axis electric translation stage 103;

the imaging component is integrated on the Z-axis electric translation stage 103, and the imaging component adjusts the position relative to the inspected material through the Z-axis electric translation stage 103;

imaging data of the exfoliated cells in the examination material collected by the imaging component is transmitted to the computer 2 and displayed by a display of the computer 2.

Specifically, the embodiment discloses an exfoliated cell discovery instrument, which mainly comprises a host platform 1, a computer 2 and an HMI operation desk 3, wherein the HMI operation desk 3 is mainly used for controlling each mechanism of the host platform 1 to work. The discovery instrument of the embodiment can realize nondestructive direct discovery of tissue cells under the condition that the material evidence is not dyed, so that the problem that the material evidence is not dyed before is solved, analysis and judgment can be carried out from biological morphological characteristics, the reliability is high, non-biological component substances can be effectively removed, and a reliable objective foundation is provided for DNA extraction and amplification in the later stage.

Secondly, the discovery apparatus of this embodiment does not determine whether the cells are exfoliated according to the characteristic of fluorescence, but may determine the relevant suspicious sites according to the morphology after imaging the suspicious sites by a certain optical imaging. Because the histiocyte has biological characteristics, the histiocyte biological characteristics can avoid false positives caused by judging from fluorescence characteristics only. Referring to fig. 1 and 2, both of the conventional fluorescence methods have fluorescence, and are suspected to be tissue cells containing DNA, and the left side is actually detected by the discovery apparatus of the present application and then is judged to be chemical synthetic substances, and have no DNA component, and are directly excluded; the right side has biological morphological characteristics, and is judged to be tissue cells containing DNA, and the next DNA examination typing can be carried out. In fig. 1, in the conventional fluorescence detection, both have fluorescence, and specific characteristics cannot be distinguished. Fig. 2 is an image of two inspection materials detected by the discovery apparatus of the present application.

Since blind extraction may cause mixed extraction, DNA typing after mixing may be difficult, and the discovery instrument of the application may realize separate respective targeted extraction, that is, avoid the problem of difficult typing of mixed separation which may be caused. Referring to fig. 3, two groups of tissue cells may be derived from different individuals, and if they cannot be extracted separately, they may cause a problem that they cannot be efficiently typed after mixing.

Since many complex inspection materials contain various materials at the same time, the discovery apparatus of this embodiment is equipped with various light sources, and can search and verify on different materials of the same inspection material by using different light sources, and can show the exfoliated cells on different materials by using the reflection and absorption characteristics of different light sources, so that the discovery rate can be greatly improved, as shown in fig. 4.

Preferably, the Z-axis electric translation stage 103 of the present embodiment is slidably connected with a slide, and a support arm 104 is mounted on the slide;

the imaging assembly is mounted to the end of arm 104 distal from the end of Z-axis motorized translation stage 103.

The present embodiment further defines the composition of a three-axis adjustment stage, which includes an X-axis motorized translation stage 101, a Y-axis motorized translation stage 102, and a Z-axis motorized translation stage 103; the structure of the triaxial adjustment platform in this embodiment is not limited, and any mechanism capable of realizing independent adjustment in the prior art, such as a screw slider mechanism, may be used to control the motors of the corresponding translation stages in a centralized manner by the controller so as to drive the corresponding platforms to move towards a specified direction to adjust the positions.

Preferably, the imaging assembly of the present embodiment includes:

an imaging module housing 105, a display screen being integrated at an upper portion of the imaging module housing 105;

a magnification-varying lens barrel 107 integrated inside the imaging module housing 105 and extending in an axial direction of the imaging module housing 105, the lower end of the magnification-varying lens barrel 107 having an imaging objective lens 108, the upper end of the magnification-varying lens barrel 108 having a high-speed camera 109;

the lower end of imaging assembly housing 105 mounts a multi-band light source system 112;

the imaging module housing 105 has a magnification adjustment mechanism 110 integrated therein, and the magnification adjustment mechanism 110 is connected to the magnification-varying barrel 107 and controls the magnification-varying barrel 107.

The imaging module housing 105 of the present embodiment has a control circuit board 111 integrated therein.

The side of the imaging assembly housing 105 of this embodiment is integrated with a double red dot positioning structure 113;

the dual red dot positioning structure 113 emits two red marker dots toward the surface of the Y-axis motorized translation stage 102.

Preferably, the multiband light source system 112 of the present embodiment mainly includes a light source housing 11201, and a light source bottom cover 11202 is provided at the bottom of the light source housing 11201. In this embodiment, a plurality of groups of light sources are integrated inside the light source system, and the light source bottom cover 11202 is provided with a hole matched with the corresponding light source. The multiple groups of light sources in the embodiment are respectively a 365nm ultraviolet light source 11203, an 850nm infrared light source 11204, a 405nm ultraviolet light source, a 525nm blue light source 11206 and a 445nm green light source 11207; and a right white light source 11210 and a left white light source 11209 are respectively disposed at both sides of the light source system.

The light source system of this embodiment is provided with a red point of the shell, and the right side of the light source system is provided with a red point on the right side. When the red spot of the shell is opened for positioning; a red light spot with the diameter of only 0.5mm irradiates on the center point of the imaging object distance; this is the actual location to be located; when the right positioning key is pressed, calculating a moving position through the XY platform, and moving the actual position of the red point positioning of the shell to the right red point positioning position; the red dot positioning is realized, and the material evidence is conveniently and effectively extracted at the right red dot positioning position;

preferably, the HMI console 3 of the present embodiment includes:

the operation table comprises an operation table shell 301, wherein a bottom plate 303 is arranged at the bottom of the operation table shell 301, a light source control screen 305 is arranged at the upper part of the operation table shell 301, and the light source control screen 305 is used for controlling the multiband light source system 112;

one side of the console shell 301 is provided with a three-dimensional control handle 302, and the three-dimensional control handle 302 is used for respectively controlling the actions of the X-axis electric translation stage 101, the Y-axis electric translation stage 102 and the Z-axis electric translation stage 103;

the console housing 301 is also provided with a scram switch 304 and a key switch 306.

The HMI console 3 is provided with an exposure adjustment knob 307 and a light source intensity adjustment knob 308.

During operation, the material evidence is placed on the Y-axis electric translation stage 102, the X-axis electric translation stage 101 and the Y-axis electric translation stage 102 are controlled to move through the three-dimensional control handle of the HMI operation stage 3 so as to move the material evidence to the lower part of the imaging assembly, and the Z-axis electric translation stage 103 is adjusted so as to control the distance between the imaging assembly and the material evidence. Then, the imaging assembly is controlled to focus, and then the magnification electric adjustment mechanism 110 is controlled to zoom in or out, wherein the light source control switch realizes the regulation and control and optical assistance of the multiband optical system 112, and then the imaging assembly is controlled to image through the high-speed camera 109 with a filter and the zoom lens, and finally the high-definition imaging is performed on the computer 2. The suspicious components on the examination material are observed by imaging on a computer 2, and the cell morphology identification is carried out by adjusting parameters such as exposure, etc. The computer 2 of the embodiment can also perform contrast identification under light sources of different wave bands, and after confirmation, the computer can perform accurate positioning through double red point positioning, and can move the detection material to the position of another positioning red point outside the lens, so that the marking and extraction operations are facilitated.

While certain exemplary embodiments of the present utility model have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that modifications may be made to the described embodiments in various different ways without departing from the spirit and scope of the utility model. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive of the scope of the utility model, which is defined by the appended claims.

Claims

1. An exfoliated cell finder, characterized in that the finder comprises:

the system comprises a host platform (1), wherein the host platform (1) is provided with a triaxial adjusting platform and an imaging assembly for checking a checking material positioned on the triaxial adjusting platform;

a computer (2) connected to the imaging assembly; and

an HMI console (3) for controlling the host platform (1);

the triaxial adjustment platform is divided into:

an X-axis electric translation stage (101), a Y-axis electric translation stage (102) and a Z-axis electric translation stage (103);

the X-axis electric translation stage (101) is positioned on the Y-axis electric translation stage (102), the detection material is placed on the Y-axis electric translation stage (102), and the detection material is adjusted in X-direction and Y-direction positions through the X-axis electric translation stage (101) and the Y-axis electric translation stage (102) so as to adjust the position relative to the Z-axis electric translation stage (103);

the imaging assembly is integrated with the Z-axis electric translation table (103), and the imaging assembly adjusts the position relative to the inspected material through the Z-axis electric translation table (103);

imaging data of the exfoliated cells in the examination material collected by the imaging component are transmitted to the computer (2) and displayed by a display of the computer (2).

2. The exfoliated cell finder according to claim 1, wherein the Z-axis electric translation stage (103) is slidingly connected with a slide, and the slide is provided with a support arm (104);

the imaging component is arranged at the end part of one end, far away from the Z-axis electric translation stage (103), of the support arm (104).

3. The exfoliated cell finder of claim 2, wherein the imaging assembly includes:

an imaging assembly housing (105), an upper portion of the imaging assembly housing (105) being integrated with a display screen;

a zoom lens barrel (107) integrated inside the imaging assembly housing (105) and extending in the axial direction of the imaging assembly housing (105), wherein an imaging objective lens (108) is arranged at the lower end of the zoom lens barrel (107), and a high-speed camera (109) is arranged at the upper end of the zoom lens barrel (107);

the lower end of the imaging assembly housing (105) is provided with a multiband light source system (112);

the imaging component shell (105) is internally integrated with a magnification adjusting mechanism (110), and the magnification adjusting mechanism (110) is connected with the zoom lens barrel (107) and controls the zoom lens barrel (107).

4. The exfoliated cell finder of claim 3, wherein the imaging assembly housing (105) has a control circuit board (111) integrated therein.

5. The exfoliated cell finder of claim 3, wherein the side of the imaging assembly housing (105) is integrated with a double red spot locating structure (113);

the dual red dot positioning structure (113) emits two red mark dots towards the surface of the Y-axis electric translation stage (102).

6. The exfoliated cell finder according to claim 3, wherein the HMI console (3) includes:

a console housing (301), wherein a bottom plate (303) is arranged at the bottom of the console housing (301), a light source control screen (305) is installed at the upper part of the console housing (301), and the light source control screen (305) is used for controlling the multiband light source system (112);

one side of the operating platform shell (301) is provided with a three-dimensional control handle (302), and the three-dimensional control handle (302) is used for respectively controlling the actions of the X-axis electric translation platform (101), the Y-axis electric translation platform (102) and the Z-axis electric translation platform (103);

the console shell (301) is also provided with a scram switch (304) and a key switch (306).

7. The exfoliated cell finder according to claim 6, wherein the HMI console (3) is provided with an exposure adjusting knob (307) and a light source intensity adjusting knob (308).