CN220587360U - A safe storage equipment for immune stem cell - Google Patents
A safe storage equipment for immune stem cell Download PDFInfo
- Publication number
- CN220587360U CN220587360U CN202321299501.5U CN202321299501U CN220587360U CN 220587360 U CN220587360 U CN 220587360U CN 202321299501 U CN202321299501 U CN 202321299501U CN 220587360 U CN220587360 U CN 220587360U
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- Prior art keywords
- fixed
- stem cell
- barrel
- stem cells
- immune
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 61
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 22
- 238000007789 sealing Methods 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 11
- 229910052757 nitrogen Inorganic materials 0.000 claims description 11
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 238000004080 punching Methods 0.000 abstract 4
- 230000037431 insertion Effects 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 9
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000004321 preservation Methods 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses safety storage equipment for immune stem cells, which comprises a supporting component, a conveying mechanism, a clamp mounting circular ring and a punching component, wherein the supporting component comprises a bottom plate and a supporting frame fixed at the top end of the bottom plate, a through rubber tube jack is formed in the side wall of the supporting frame, and a through punching hole is formed in the top end of the supporting frame; the conveying mechanism comprises a rotating rod symmetrically and rotatably arranged on the bottom plate, a conveying wheel fixed on the rotating rod and a driving assembly for driving the rotating rod to rotate; the clamp mounting circular ring is arranged on the side edge of the support frame and coaxial with the rubber tube insertion hole, mounting plates are symmetrically fixed on the clamp mounting circular ring, electric telescopic rods are fixed on the mounting plates, and tool clamping blocks are fixed at the ends of the electric telescopic rods; the punching component is arranged on the supporting frame, can punch the rubber tube, can automatically punch the rubber tube continuously, and has accurate punching position.
Description
Technical Field
The utility model relates to the field of safety storage equipment of immune stem cells, in particular to safety storage equipment for immune stem cells.
Background
At present, cell storage is a way of preserving cells, and the preserved cells can be subsequently cultured, so that the sterility of the surrounding environment needs to be ensured in the cell culture and storage processes, and the environment of a laboratory needs to be disinfected at regular time.
At present, a mode of vacuum and liquid nitrogen low-temperature preservation is adopted for preservation of cells, however, the preserved cells need to be frequently taken in a laboratory to carry out culture experiments, the sealing cover of the device is mostly opened from the top when the cells are taken, then the stem cell storage box is taken out from the bottom of the device, and in the process of taking out the stem cells, the sealing cover of the device is always opened, so that a large amount of liquid nitrogen in the storage device is lost, the internal low-temperature environment is influenced, and the preservation of the cells is influenced.
Disclosure of Invention
The utility model aims to provide a safe storage device for immune stem cells, which solves the problems in the background technology.
In order to achieve the above purpose, the utility model provides a safe storage device for immune stem cells, which comprises an external protection barrel, an internal storage barrel and a stem cell placing frame.
The outer protection barrel is provided with a vacuumizing pipe communicated with the inner cavity of the outer protection barrel, and the top of the outer protection barrel is provided with an annular baffle;
the storage device comprises an inner storage barrel, a guide rod and a guide rod, wherein the inner storage barrel is fixed in an inner cavity of the outer protection barrel, a storage hole is formed in the top of the inner storage barrel, a sealing cover is hinged to the storage hole, a liquid nitrogen injection pipe is fixed to the top of the inner storage barrel, a threaded rod is rotatably arranged on the inner storage barrel, and the guide rod is fixed to the inner part of the inner storage barrel;
the stem cell rack is placed in the inner cavity of the internal storage barrel, slots for placing stem cell boxes are formed in the side walls of the stem cell rack, mounting plates are symmetrically fixed on the side walls of the stem cell rack, threaded holes meshed with the threaded rods are formed in one mounting plate, and guide holes sleeved on the guide rods are formed in the other mounting plate.
As a preferred scheme of the safety storage device for immune stem cells, the inner wall of the sealing cover is symmetrically fixed with a first fixing plate, and the fixing plate is fixed with a first protruding column.
As a preferable scheme of the safety storage device for immune stem cells, the top end of the stem cell placing rack is symmetrically fixed with a second fixing plate, and the second fixing plate is fixed with a second protruding column.
As a preferred scheme of the safety storage device for immune stem cells, the first protruding column is rotatably provided with a rotating rod, the rotating rod is provided with a waist-shaped hole, and the second protruding column is inserted into the waist-shaped hole.
As a preferred scheme of the safe storage device for immune stem cells, a rotary disc is fixed at the outer end of the threaded rod.
Compared with the prior art, the utility model has the following beneficial effects:
the stem cell storage box can be lifted to the lower side of the sealing cover of the device in advance, then the sealing cover is opened, and the sealing cover is closed after the stem cell storage box is lifted and taken out, so that the time for opening the sealing cover is shorter, and a great amount of loss of liquid nitrogen is avoided.
Drawings
Other features, objects and advantages of the present utility model will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:
FIG. 1 is a schematic diagram of the overall structure of a safety storage device for immune stem cells according to the present utility model;
FIG. 2 is a schematic structural view of an external protective barrel for immune stem cells according to the present utility model;
FIG. 3 is a schematic diagram of the structure of an internal storage tank for immune stem cells according to the present utility model;
FIG. 4 is a cutaway view of an internal storage bucket for immune stem cells of the present utility model;
FIG. 5 is a schematic diagram of a closure for an immune stem cell according to the present utility model;
FIG. 6 is a schematic diagram of a stem cell holder for immune stem cells according to the present utility model.
In the figure: 100. an outer protection tub; 100a, vacuumizing tube; 100b, annular baffles; 200. an internal storage tub; 200a, a storage hole; 200b, sealing; 200b-1, a first fixing plate; 200b-2, a first post; 200b-3, rotating lever; 200b-4, waist-shaped holes; 200c, a liquid nitrogen injection pipe; 200d, a threaded rod; 200d-1, a turntable; 200e, a guide rod; 300. a stem cell rack; 300a, a slot; 300b, mounting plate; 300c, a threaded hole; 300d, guiding holes; 300e, a second fixing plate; 300f, second posts.
Detailed Description
The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.
Example 1
Fig. 1 to 6 are schematic structural views showing an embodiment of a safety storage device for immune stem cells according to the present utility model, referring to fig. 1 to 6, a safety storage device for immune stem cells according to the present utility model, the main body of which includes an outer protective tub 100, an inner tub 200, and a stem cell holder 300.
The side wall of the outer protection barrel 100 is fixed with an evacuating pipe 100a communicated with the inner cavity of the outer protection barrel 100, and the top is fixed with an annular baffle 100b;
the inner storage barrel 200 is fixed in the inner cavity of the outer protection barrel 100, a storage hole 200a is formed in the top of the inner storage barrel 200, a sealing cover 200b is hinged to the storage hole 200a, a liquid nitrogen injection pipe 200c is fixed to the top of the inner storage barrel 200, a threaded rod 200d is rotatably installed on the inner storage barrel 200, and a guide rod 200e is fixed to the inner part of the inner storage barrel 200;
the stem cell placing rack 300 is placed in the inner cavity of the internal storage barrel 200, a slot 300a for placing a stem cell box is formed in the side wall of the stem cell placing rack, mounting plates 300b are symmetrically fixed on the side wall, a threaded hole 300c meshed with the threaded rod 200d is formed in one mounting plate 300b, and a guide hole 300d sleeved on the guide rod 200e is formed in the other mounting plate 300 b.
Specifically, firstly, the stem cell box is inserted into the slot 300a for placement, then the threaded rod 200d is rotated to drive the stem cell rack 300 to move into the inner cavity of the inner storage barrel 200, then the sealing cover 200b is closed, then liquid nitrogen is injected into the inner cavity of the inner storage barrel 200 through the liquid nitrogen injection pipe 200c, so that the stem cells are preserved in a low-temperature environment, and then the vacuum is pumped between the outer protective barrel 100 and the inner storage barrel 200 through the vacuumizing pipe 100a, so that the temperature exchange between the inner storage barrel 200 and the external environment is reduced. When stem cells need to be taken, the threaded rod 200d is rotated to drive the stem cell placing frame 300 to ascend, and then the sealing cover 200b is opened, so that the stem cell box can be directly taken after the sealing cover 200b is opened, the opening time of the sealing cover 200b is reduced, the loss of liquid nitrogen is reduced, after the stem cell box is taken out, the stem cell placing frame 300 is placed back, the sealing cover 200b is closed, and the phenomenon that the liquid nitrogen is lost due to the fact that the process of taking the stem cell box out of the bottom after the sealing cover 200b is opened is long is avoided.
Further, when the stem cell holder 300 is lifted up below the cover 200b, it is also necessary to manually open the cover 200b, and then manually close the cover 200b after taking out the stem cell cassette, which increases the manpower loss.
On the basis of this, the first fixing plate 200b-1 is symmetrically fixed to the inner wall of the cover 200b, and the first protrusion 200b-2 is fixed to the fixing plate 200 b-1.
The stem cell holder 300 is symmetrically fixed with a second fixing plate 300e at the top end, and a second protruding column 300f is fixed on the second fixing plate 300 e.
The first protruding column 200b-2 is rotatably provided with a rotating rod 200b-3, the rotating rod 200b-3 is provided with a waist-shaped hole 200b-4, and the second protruding column 300f is inserted into the waist-shaped hole 200 b-4.
When the stem cells are taken, the threaded rod 200d is rotated to drive the stem cell placing frame 300 to ascend, the second protruding column 300f is propped against the top end of the waist-shaped hole 200b-4 and continuously jacks up the rotating rod 200b-3, when the second fixing plate 300e is upwards protruded outside the storage hole 200a, the rotating rod 200b-3 is jacked up to rotate, the sealing cover 200b is rotated, then the stem cell placing frame 300 is exposed outside the internal storage barrel 200, at the moment, the cell box can be manually taken out, finally, the threaded rod 200d is reversely rotated to drive the stem cell placing frame 300 to reset, the second protruding column 300f drives the rotating rod 200b-3 to reset, and the sealing cover 200b is closed, so that the sealing cover 200b does not need to be manually opened or closed in the process of taking the cell box. And in case that the threaded rod 200d is not rotated in the correct direction, the cap 200b is not opened by the erroneous touch.
While the fundamental principles, principal features, and advantages of the present utility model have been shown and described, it will be apparent to those skilled in the art that the present utility model is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (5)
1. A secure storage device for immune stem cells, comprising:
an evacuating pipe (100 a) communicated with the inner cavity of the external protection barrel (100) is fixed on the side wall of the external protection barrel (100), and an annular baffle (100 b) is fixed on the top of the external protection barrel;
the inner storage barrel (200) is fixed in the inner cavity of the outer protection barrel (100), a storage hole (200 a) is formed in the top of the inner storage barrel, a sealing cover (200 b) is hinged to the storage hole (200 a), a liquid nitrogen injection pipe (200 c) is fixed to the top of the inner storage barrel (200), a threaded rod (200 d) is rotatably arranged on the inner storage barrel (200), and a guide rod (200 e) is fixed to the inner part of the inner storage barrel (200);
the stem cell placing frames (300) are placed in the inner cavity of the internal storage barrel (200), slots (300 a) for placing stem cell boxes are formed in the side walls of the stem cell placing frames, mounting plates (300 b) are symmetrically fixed on the side walls of the stem cell placing frames, threaded holes (300 c) meshed with the threaded rods (200 d) are formed in one mounting plate (300 b), and guide holes (300 d) sleeved on the guide rods (200 e) are formed in the other mounting plate (300 b).
2. The safety storage device for immune stem cells according to claim 1, wherein a first fixing plate (200 b-1) is symmetrically fixed on an inner wall of the cover (200 b), and a first stud (200 b-2) is fixed on the fixing plate (200 b-1).
3. The safety storage device for immune stem cells according to claim 2, wherein a second fixing plate (300 e) is symmetrically fixed to the top end of the stem cell holder (300), and a second protruding column (300 f) is fixed to the second fixing plate (300 e).
4. A safety storage device for immune stem cells according to claim 3, wherein a rotating rod (200 b-3) is rotatably mounted on the first protruding column (200 b-2), a waist-shaped hole (200 b-4) is formed on the rotating rod (200 b-3), and the second protruding column (300 f) is inserted into the waist-shaped hole (200 b-4).
5. A safety storage device for immune stem cells according to claim 2, characterized in that the outer end of the threaded rod (200 d) is fixed with a turntable (200 d-1).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202321299501.5U CN220587360U (en) | 2023-05-26 | 2023-05-26 | A safe storage equipment for immune stem cell |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202321299501.5U CN220587360U (en) | 2023-05-26 | 2023-05-26 | A safe storage equipment for immune stem cell |
Publications (1)
Publication Number | Publication Date |
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CN220587360U true CN220587360U (en) | 2024-03-15 |
Family
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Family Applications (1)
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CN202321299501.5U Active CN220587360U (en) | 2023-05-26 | 2023-05-26 | A safe storage equipment for immune stem cell |
Country Status (1)
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CN (1) | CN220587360U (en) |
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2023
- 2023-05-26 CN CN202321299501.5U patent/CN220587360U/en active Active
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