CN220584427U - Yellow-green fluorescent imaging coated lens - Google Patents

Abstract

The utility model discloses a film-coated lens for yellow-green fluorescent imaging, which comprises: the anti-reflection coating comprises a substrate and an anti-reflection coating layer, wherein the anti-reflection coating layer is arranged on the outer side of the substrate and comprises a seven-layer structure from inside to outside in sequence, and the thickness coefficient ratio among a first low refractive index film, a second high refractive index film, a third low refractive index film, a fourth high refractive index film, a fifth low refractive index film, a sixth high refractive index film and a seventh low refractive index film is 0.17:0.44:0.79:1.44:0.11:1.78:2.30. Aiming at the problem of detail loss caused by weak luminescence in the yellow-green fluorescence imaging process, the application prepares the visible light band yellow-green light high-pass type antireflection film by taking the F52R material in the cycloolefin copolymer as a base lens, and test results show that: when the incident angle of the light is 5 degrees, the average reflectivity of 400-700 nm is about 0.55 percent, the average reflectivity of 500-600 nm is about 0.29 percent, and the spectrum curve shows the shape of yellow-green light high pass so as to meet the use requirement.

Description

Yellow-green fluorescent imaging coated lens

Technical Field

The utility model relates to the field of lens manufacturing, in particular to a film-coated lens for yellow-green fluorescent imaging.

Background

The fluorescent dye has the characteristics of absorbing light energy with certain specific wavelength and emitting light with other wavelengths (generally longer wave bands), and for different substances, the fluorescent characteristic also shows different characteristics, can intuitively reflect the detected substances, and realizes qualitative or quantitative analysis. The fluorescent marking and fluorescent probe technology is used for detecting and analyzing biomolecules, cells and various tissues which are difficult to directly observe by naked eyes according to the principle, forms a visual image with rich information, and has better application prospect in the aspects of biomolecule detection imaging, drug distribution and metabolism tracking, tumor and detection and early diagnosis of various lesions, and the like. The fluorescence in the visible light band is easily disturbed, possibly resulting in loss of details, due to the fact that the conversion efficiency of the fluorescent substance is generally not very high and the environment in which the observed object is located is complex. In addition, the identification and analysis of plant types and lesion cells can be performed according to the autofluorescence phenomenon of some plants, and the type identification can be performed according to the fluorescence characteristics of the plants.

Since the spectral curve of the antireflection film in the visible light band at present mostly belongs to the low-pass green light (that is, the reflectivity of green light is higher than that of other colors), for a system for observing yellow-green fluorescence imaging, the slight yellow and green reflections can cause the tiny structure with weak luminescence to be ignored because of no observation.

Disclosure of Invention

The utility model aims to provide a film-coated lens for yellow-green fluorescent imaging to solve the problems.

According to one aspect of the present utility model, there is provided a coated lens for yellow-green fluorescent imaging, comprising: the antireflection film layer is arranged on the outer side of the substrate and comprises seven layers of structures from inside to outside, namely a first layer of low refractive index film, a second layer of high refractive index film, a third layer of low refractive index film, a fourth layer of high refractive index film, a fifth layer of low refractive index film, a sixth layer of high refractive index film and a seventh layer of low refractive index film; the thickness coefficient ratio among the first layer low refractive index film, the second layer high refractive index film, the third layer low refractive index film, the fourth layer high refractive index film, the fifth layer low refractive index film, the sixth layer high refractive index film and the seventh layer low refractive index film is 0.17:0.44:0.79:1.44:0.11:1.78:2.30.

In some embodiments, the substrate is an F52R lens.

In some embodiments, the first, third, fifth, and seventh low index films are silicon dioxide.

In some embodiments, the second, fourth, and sixth high refractive index films are titanium pentoxide.

Compared with the prior art, the beneficial effects of this application are:

aiming at the problem of detail loss caused by weak luminescence in the yellow-green fluorescence imaging process, the application prepares the visible light band yellow-green light high-pass type antireflection film by taking the F52R material in the cycloolefin copolymer as a base lens, and test results show that: when the incident angle of the light is 5 degrees, the average reflectivity of 400-700 nm is about 0.55 percent, the average reflectivity of 500-600 nm is about 0.29 percent, and the spectrum curve shows the shape of yellow-green light high pass so as to meet the use requirement.

Drawings

FIG. 1 is a graph of spectral reflectance for the present utility model;

FIG. 2 is a graph of the measured reflectance spectrum at an angle of incidence of 5 according to the present utility model;

fig. 3 is a schematic cross-sectional structure of the present utility model.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1 to 3, the present application provides a coated lens for yellow-green fluorescent imaging, comprising: the anti-reflection film layer is arranged on the outer side of the substrate 1 and comprises a first low refractive index film 2, a second high refractive index film 3, a third low refractive index film 4, a fourth high refractive index film 5, a fifth low refractive index film 6, a sixth high refractive index film 7 and a seventh low refractive index film 8 which are sequentially arranged from inside to outside; wherein the thickness coefficient ratio among the first layer low refractive index film 2, the second layer high refractive index film 3, the third layer low refractive index film 4, the fourth layer high refractive index film 5, the fifth layer low refractive index film 6, the sixth layer high refractive index film 7 and the seventh layer low refractive index film 8 is 0.17:0.44:0.79:1.44:0.11:1.78:2.30.

In some embodiments, the substrate 1 is an F52R lens, and the cycloolefin copolymer (Cyclic Olefin Copolymer), abbreviated as COC, is a novel amorphous transparent copolymer material, has excellent properties such as high transparency, high temperature stability, low birefringence, moisture resistance, high rigidity, and the like, and is an excellent material for manufacturing an optical plastic lens.

In some embodiments, the first, third, fifth, and seventh low refractive index films 2, 4, 6, 8 are silicon dioxide.

In some embodiments, the second, fourth, and sixth high refractive index films 3, 5, 7 are titanium pentoxide.

The spectrum theory reflectivity curve of the application is shown in figure 1, and the high-pass treatment is carried out on the yellow-green light with the wavelength of 500-600 nm.

Since the F52R lens is an optical plastic, the release of the film is easily caused in the case of low-temperature cold plating, and thus the adhesion of the first low refractive index film 2 to the F52R lens surface and the bonding force between adjacent films of each layer are considered. The adhesion force of the film material to the substrate 1 adopts an ion pretreatment process to activate the substrate 1, so as to improve the surface binding force. Through continuous experiments, the ion pretreatment time is finally determined to be 500s. Since the F52R lens has a heat distortion temperature of 156 ℃, atoms cannot be energized by high temperature heating, and therefore the ion source is selected to assist deposition at room temperature.

The poor binding force between the film materials is caused by the existence of film stress, the stress accumulated in the film is weakened by adopting alternate preparation of compressive stress and tensile stress, the stress between the film layers cannot be released due to the fact that the silicon dioxide and the titanium pentoxide are both compressive stress, and the optical performance of the film is finally affected by the stress, so that the film is treated by a process of adjusting the energy of an ion source, namely the film is bombarded by adopting lower energy of the ion source, a loose structure is formed, excessive concentration of the stress is prevented to a certain extent, the final process parameters are shown in table 1, and the ion source parameters are shown in table 2.

Table 1 process parameters for film preparation

TABLE 2 ion Source parameters

Before film plating, the F52R lens is baked and dried, and baked for 2 hours at 80 ℃, so that the water vapor on the surface can be removed to the greatest extent, and the internal stress is reduced. In the preparation process, in order to eliminate the influence of the air release amount of the film material and some impurities, the titanium pentoxide material needs to be premelted, and the premelted is not needed because the silicon dioxide has good evaporation stability.

The spectrum test is carried out on the antireflection film plated at the wave band of 400-700 nm by adopting a standard mark plate-3000 ultraviolet/visible/near infrared spectrophotometer at the incident angle of 5 degrees, and the test result is shown in figure 2.

The measured reflectance is about 0.55% in the wavelength range of 400-700 nm, the average reflectance of the yellow-green light is about 0.29% in the wavelength range of 500-600 nm, the spectrum curve shows the high-pass shape of the yellow-green light, and the measured spectrum curve of the plated lens basically accords with the design curve.

And (3) testing the environmental reliability, wherein the test result is as follows:

(1) Adhesion test: the film layer was adhered to the surface of the film layer using a clear adhesive tape having a width of 2cm, and after the adhesive tape was rapidly pulled up from the direction perpendicular to the substrate 1, the film layer was free from peeling.

(2) Constant temperature and humidity test: and the film layer cracking phenomenon is avoided by observing the film layer cracking phenomenon through a microscope in a constant temperature and humidity box with the temperature of 50 ℃ and the humidity of 75% for 12 hours.

Aiming at the problem of detail loss caused by weak luminescence in the yellow-green fluorescence imaging process, the F52R material in the cycloolefin copolymer is used as a substrate 1 to prepare the visible light band yellow-green light high-pass type antireflection film, and test results show that: when the incident angle of the light is 5 degrees, the average reflectivity of 400-700 nm is about 0.55 percent, the average reflectivity of 500-600 nm is about 0.29 percent, and the spectrum curve shows the shape of yellow-green light high pass so as to meet the use requirement.

Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present utility model has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (4)

1. A coated lens for yellow-green fluorescent imaging, comprising: the antireflection film layer is arranged on the outer side of the substrate and comprises seven layers of structures from inside to outside, namely a first layer of low refractive index film, a second layer of high refractive index film, a third layer of low refractive index film, a fourth layer of high refractive index film, a fifth layer of low refractive index film, a sixth layer of high refractive index film and a seventh layer of low refractive index film;

the thickness coefficient ratio among the first layer low refractive index film, the second layer high refractive index film, the third layer low refractive index film, the fourth layer high refractive index film, the fifth layer low refractive index film, the sixth layer high refractive index film and the seventh layer low refractive index film is 0.17:0.44:0.79:1.44:0.11:1.78:2.30.

2. The yellow-green fluorescent imaging coated lens of claim 1, wherein the substrate is an F52R lens.

3. The film-coated lens for yellow-green fluorescence imaging according to claim 1, wherein the first low refractive index film, the third low refractive index film, the fifth low refractive index film, and the seventh low refractive index film are silica.

4. The film-coated lens for yellow-green fluorescence imaging according to claim 1, wherein the second high refractive index film, the fourth high refractive index film, and the sixth high refractive index film are titanium pentoxide.