CN220579246U - Temperature-stable bioreactor - Google Patents
Temperature-stable bioreactor Download PDFInfo
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- CN220579246U CN220579246U CN202322033142.5U CN202322033142U CN220579246U CN 220579246 U CN220579246 U CN 220579246U CN 202322033142 U CN202322033142 U CN 202322033142U CN 220579246 U CN220579246 U CN 220579246U
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- water inlet
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 167
- 230000001105 regulatory effect Effects 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 239000000498 cooling water Substances 0.000 claims description 15
- 239000011521 glass Substances 0.000 claims description 6
- 238000005192 partition Methods 0.000 claims 2
- 230000006641 stabilisation Effects 0.000 claims 1
- 238000011105 stabilization Methods 0.000 claims 1
- 238000003860 storage Methods 0.000 description 11
- 238000000926 separation method Methods 0.000 description 9
- 238000004113 cell culture Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 238000001816 cooling Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a temperature-stable bioreactor, which comprises a base, wherein a tank body is arranged on the base, a stirring assembly is arranged above the tank body, a jacket is arranged on the outer side of the tank body, a temperature control mechanism is further arranged between the base and the tank body, a water inlet pipe and a water outlet pipe of the temperature control mechanism are respectively connected with a water inlet and a water outlet of the jacket, and the temperature control mechanism further comprises a pressure adjusting assembly arranged between the water inlet pipe and the water outlet pipe. Compared with the prior art, the temperature control mechanism is arranged between the base and the tank body, is managed in a centralized way, and is convenient to maintain. Secondly, a layer of jacket capable of being communicated with water is added on the tank body, so that the tank body can be stabilized at a temperature for a long time. Thirdly, by arranging the pressure regulating component, the pressure stability of the bioreactor can be effectively maintained.
Description
Technical Field
The utility model relates to the technical field of bioreactors, in particular to a bioreactor with stable temperature.
Background
The tank body of the bioreactor is an important part of the bioreactor, can provide a good environment for cell culture, and has wide application in the biopharmaceutical industry. The patent with the publication number of CN211554768U discloses a heat exchange cavity temperature control system of a bioreactor, which comprises the bioreactor, a cold heat exchanger, a hot heat exchanger, a pressure gauge, a circulating pump, a water storage tank and a temperature sensor, wherein a first temperature sensor is arranged in the bioreactor, and a temperature control heat exchange cavity is arranged at the lower part of the bioreactor; the temperature control system of the heat exchange cavity further comprises a PLC control system, the PLC control system is connected with the winding pipe electric heater, and the second temperature sensor is arranged on the water outlet pipeline between the winding pipe electric heater and the temperature control heat exchange cavity. The stirring component of the temperature control system is arranged below the bioreactor, the cell culture solution is directly cultured in the bioreactor, and secondly, the system is provided with two sets of heat exchangers, so that the volume and the manufacturing cost of the system are increased.
Disclosure of Invention
The utility model provides a temperature-stable bioreactor which is used for solving the problem that a temperature control system in the prior art is inconvenient to manage and maintain for separate design.
The utility model provides a temperature-stable bioreactor which comprises a base, wherein a tank body is arranged on the base, a stirring assembly is arranged above the tank body, a jacket is arranged on the outer side of the tank body, a temperature control mechanism is further arranged between the base and the tank body, a water inlet pipe and a water outlet pipe of the temperature control mechanism are respectively connected with a water inlet and a water outlet of the jacket, and the temperature control mechanism further comprises a pressure adjusting assembly arranged between the water inlet pipe and the water outlet pipe.
Preferably, the pressure regulating assembly is an expansion tank.
Preferably, the temperature control mechanism comprises a water pump and a heater, the expansion tank is connected with the water outlet of the jacket through a water outlet pipe, the heater is connected with the water inlet of the jacket through a water inlet pipe, and the water pump is arranged on a pipeline between the heater and the expansion tank.
Preferably, the temperature control mechanism further comprises a heat exchanger and a temperature sensor, wherein the heat exchanger is arranged on a pipeline between the expansion tank and the water pump, and the temperature sensor is arranged on a pipeline between the heater and the water inlet.
Preferably, the water pump further comprises a water inlet pipe, and the water inlet pipe is connected to a pipeline between the heat exchanger and the water pump through a switch valve.
Preferably, a safety valve is arranged on a pipeline between the expansion tank and the heat exchanger, and the upper end of the jacket is connected with a sight glass.
Preferably, the expansion tank further comprises a drain pipe, and the drain pipe is connected to a pipeline between the water outlet and the expansion tank through a switch valve.
Preferably, the heat exchanger is connected with a first cooling water pipe and a second cooling water pipe, an electromagnetic valve is arranged on the first cooling water pipe, and a tuning fork level gauge is arranged at the upper end of the heater.
Preferably, a separation area is arranged at the bottom of the jacket, two chambers are arranged in the separation area and are respectively communicated with the water inlet pipe and the water outlet pipe, the water outlet is communicated with the chamber connected with the water outlet pipe through a drainage groove, and the water inlet pipe is communicated with the other chamber through a water inlet.
Preferably, a plurality of sealing strips are arranged in the jacket from top to bottom, the sealing strips divide the jacket into a plurality of heat exchange cavities, water through holes are formed in the sealing strips, and the upper water through hole and the lower water through hole are respectively positioned at two ends of the heat exchange cavities.
Compared with the prior art, the temperature control mechanism is arranged between the base and the tank body, is managed in a centralized way, and is convenient to maintain. Secondly, a layer of jacket capable of being communicated with water is added on the tank body, and then a heater and a heat exchanger are connected on the jacket, so that the tank body is stabilized at a temperature for a long time. Thirdly, by arranging the pressure regulating component, the pressure stability of the bioreactor can be effectively maintained.
Drawings
In order to more clearly illustrate the utility model or the technical solutions of the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the utility model, and other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is an enlarged schematic view of the structure shown at A in FIG. 1;
FIG. 3 is a schematic view of another view of the present utility model;
FIG. 4 is a schematic view of a portion of the structure of the present utility model;
FIG. 5 is a schematic view of the structure of the can body of the present utility model with the outer shell removed;
fig. 6 is a schematic view of another view of fig. 5.
Reference numerals:
1. the water tank comprises a tank body, a jacket, a water outlet pipe, a water inlet pipe, a heat exchanger, a water pump, a heater, a temperature sensor, a water inlet pipe, an expansion tank, a switching valve, a safety valve, a sight glass, a water outlet pipe, a first cooling water pipe, a second cooling water pipe, a solenoid valve, a tuning fork level gauge, a magnetic valve, a tuning fork level gauge, a separation area, a water outlet pipe, a water outlet tank, a stirring assembly, a base, a heat exchange cavity, a seal, a water inlet hole, a water inlet pipe and a water outlet.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1 and 6, this embodiment provides a temperature-stable bioreactor, which comprises a base 22, wherein a tank 1 is arranged on the base 22, a stirring assembly 21 is arranged above the tank 1, the lower end of the stirring assembly 21 stretches into the tank 1, a jacket 2 is arranged on the outer side of the tank 1, the tank 1 comprises an outer shell and an inner shell, and the jacket 2 is arranged between the outer shell and the inner shell. A temperature control mechanism is further arranged between the base 22 and the tank body 1, a water inlet pipe 4 and a water outlet pipe 3 of the temperature control mechanism are respectively connected with a water inlet 400 and a water outlet 500 of the jacket 2, and the temperature control mechanism further comprises a pressure adjusting component arranged between the water inlet pipe 4 and the water outlet pipe 3. The pressure regulating component is used for regulating the pressure of water in the pipeline, specifically, the water outlet 500 and the water inlet 400 of the jacket 2 are respectively arranged at the upper end and the lower end of the tank body 1, and the pressure regulating component is an expansion tank 10.
Referring to fig. 1, the temperature control mechanism includes a water pump 6 and a heater 7, the heater 7 is used for heating a medium, the expansion tank 10 is connected with the water outlet 500 of the jacket 2 through the water outlet pipe 3, the heater 7 is connected with the water inlet 400 of the jacket 2 through the water inlet pipe 4, and the water pump 6 is arranged on a pipeline between the heater 7 and the expansion tank 10.
Referring to fig. 2, the temperature control mechanism further includes a heat exchanger 5 and a temperature sensor 8, the temperature sensor 8 is used for detecting the temperature of the medium in the pipeline, the heat exchanger 5 cools the medium through external cooling water, the heat exchanger 5 is arranged on the pipeline between the expansion tank 10 and the water pump 6, and the temperature sensor 8 is arranged on the pipeline between the heater 7 and the water inlet 400. According to the medium temperature detected by the temperature sensor 8, the temperature of the medium is regulated by heating the heater 7 and cooling the heat exchanger 5, so that the temperature of the jacket 2 is kept within a certain range, and secondly, the lower water inlet and upper water outlet modes are beneficial to making the temperature distribution of the jacket 2 uniform.
Specifically, the water inlet pipe 9 is connected to a pipeline between the heat exchanger 5 and the water pump 6 through the switch valve 11, water is firstly fed, the switch valve 11 is opened, and water is conveyed into the jacket 2 through the water pump 6. After filling with water, the water inlet pipe 9 is closed, water circulates between the jacket 2, the heat exchanger 5, the water pump 6 and the heater 7, and the expansion tank 10 regulates the pressure of the water in the pipeline in real time.
Specifically, the expansion tank 10 is provided on a pipe between the water outlet 500 and the heat exchanger 5.
Specifically, a safety valve 12 is provided on the pipe between the expansion tank 10 and the heat exchanger 5.
As another embodiment of the present utility model: the upper end of the jacket 2 is connected with a sight glass 13, when water is fed for the first time, whether the water is full is judged through the sight glass 13, the switch valve 11 is closed after the water is full, and a ball valve is arranged on the sight glass 13.
The present embodiment further includes a drain pipe 14, and the drain pipe 14 is connected to a pipe between the water outlet 500 and the expansion tank 10 through the on-off valve 11. After the cell culture is completed, the water in the jacket 2 is discharged by opening the on-off valve 11 on the drain pipe 14.
Specifically, the heat exchanger 5 is connected with a first cooling water pipe 15 and a second cooling water pipe 16, an electromagnetic valve 17 is arranged on the first cooling water pipe 15, when the temperature is too high, the electromagnetic valve 17 is opened, and the heat exchanger 5 cools the water in the jacket 2 through the cooling water in the first cooling water pipe 15 and the second cooling water pipe 16.
As another embodiment of the present utility model: the upper end of the heater 7 is provided with a tuning fork level gauge 18.
As another embodiment of the present utility model: referring to fig. 3, a separation area 19 is communicated with the bottom of the jacket 2, two chambers are arranged in the separation area 19 and are respectively communicated with the water inlet pipe 4 and the water outlet pipe 3, the water outlet 500 is communicated with the chamber connected with the water outlet pipe 3 through a water outlet groove 20, the water inlet pipe 4 is communicated with the other chamber through a water inlet 400, the water outlet groove 20 is vertically arranged on the tank body 1, the chamber connected with the water inlet pipe 4 is communicated with the jacket 2, the jacket 2 forms a gap, the water outlet groove 20 is positioned in the gap, the water outlet groove 20 and the jacket 2 form a cylinder shape, and the water outlet groove 20 is sleeved on the outer side of the tank body 1. Through this setting, make water inlet pipe 4 and outlet pipe 3 all set up the lower extreme at jar body 1, be convenient for concentrate the setting with the pipeline.
As another embodiment of the present utility model: referring to fig. 4-5, a plurality of seals 200 are arranged in the jacket 2 from top to bottom, the seals 200 divide the jacket 2 into a plurality of heat exchange cavities 100, water through holes 300 are arranged on the seals 200, the upper water through hole 300 and the lower water through hole 300 are respectively positioned in the heat exchange cavities 100, i.e. one water through hole 300 is positioned at the left end of the heat exchange cavity 100, and the other water through hole 300 is positioned at the right end of the heat exchange cavity 100. This structural design is advantageous for making the water temperature in the jacket 2 uniform throughout.
As another embodiment of the present utility model: the heat exchange cavity 100 at the bottom is divided into two water storage cavities, the two water storage cavities are respectively communicated with the two separated cavities, the water outlet 500 flows into the water storage cavities through the water drainage groove 20, then flows into the cavities from the water storage cavities, and then flows into the water outlet pipe 3 from the cavities. The water of the water inlet pipe 9 flows into the other chamber, then enters the other water storage cavity from the chamber and then enters the heat exchange cavity 100 from the water storage cavity.
In the utility model, the switch valve 11 on the water inlet pipe 9 is opened, the water pump 6 sequentially conveys water to the heater 7, the water inlet pipe 4, the separation area 19 and the water storage cavity, then enters the lower heat exchange cavity 100, is conveyed layer by layer along the water through holes 300 and is conveyed upwards until reaching the water outlet 500 at the upper end, and in the process, the heater 7 heats the water. Then flows into the water discharge groove 20 from the water outlet 500, sequentially enters the water storage cavity, the separation area 19 and the water outlet pipe 3 along the water discharge groove 20, and closes the switch valve 11 of the water inlet pipe 9 after the water is filled. Then, water circulation is started, the water pump 6 sequentially conveys water from the heat exchanger 5 to the heater 7, the water inlet pipe 4, the separation area 19 and the water storage cavity, then sequentially passes through the heat exchange cavity 100 from bottom to top, then sequentially passes through the water drainage tank 20, the water storage cavity, the separation area 19, the water outlet pipe 3 and the heat exchanger 5 after exiting from the water outlet 500, and then sequentially conveys the water to the heater 7 from the heat exchanger 5 again and again. In this process, the temperature of the water is adjusted according to the temperature detected by the temperature sensor 8, specifically by means of heating by the heater 7 and cooling by the heat exchanger 5, until the temperature is adjusted to the target temperature, thereby providing a stable environment for cell culture. The temperature control mechanism is arranged between the base 22 and the tank body 1, so that centralized management and convenient maintenance are realized. Secondly, a layer of jacket 2 capable of being communicated with water is added on the tank body 1, and then a heater 7 and a heat exchanger 5 are connected on the jacket 2, so that the tank body 1 is stabilized at a temperature for a long time. Thirdly, compared with the prior art, one heat exchanger 5 is omitted, the structure is simple, and the manufacturing cost is low. Fourth, by providing a pressure regulating assembly, the pressure stability of the bioreactor can be effectively maintained.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model.
Claims (10)
1. The utility model provides a temperature stabilization's bioreactor, includes base (22), be equipped with jar body (1) on base (22), the top of jar body (1) is equipped with stirring subassembly (21), the outside of jar body (1) is equipped with jacket (2), its characterized in that, still be equipped with temperature control mechanism between base (22) and jar body (1), water inlet pipe (4) and outlet pipe (3) of temperature control mechanism are connected with water inlet (400) and delivery port (500) of jacket (2) respectively, temperature control mechanism is still including setting up the pressure regulating subassembly between water inlet pipe (4) and outlet pipe (3).
2. A temperature-stabilized bioreactor according to claim 1, characterized in that the pressure regulating assembly is an expansion tank (10).
3. The temperature-stabilized bioreactor according to claim 2, characterized in that the temperature control mechanism comprises a water pump (6) and a heater (7), the expansion tank (10) is connected with the water outlet (500) of the jacket (2) through the water outlet pipe (3), the heater (7) is connected with the water inlet (400) of the jacket (2) through the water inlet pipe (4), and the water pump (6) is arranged on a pipeline between the heater (7) and the expansion tank (10).
4. A temperature-stabilized bioreactor as claimed in claim 3, characterized in that the temperature control mechanism further comprises a heat exchanger (5) and a temperature sensor (8), the heat exchanger (5) being arranged on the pipe between the expansion tank (10) and the water pump (6), the temperature sensor (8) being arranged on the pipe between the heater (7) and the water inlet (400).
5. The temperature-stabilized bioreactor according to claim 4, further comprising a water inlet pipe (9), said water inlet pipe (9) being connected to a pipe between the heat exchanger (5) and the water pump (6) by means of an on-off valve (11).
6. The temperature-stabilized bioreactor as claimed in claim 4, characterized in that a safety valve (12) is arranged on the pipeline between the expansion tank (10) and the heat exchanger (5), and a sight glass (13) is connected to the upper end of the jacket (2).
7. The temperature-stabilized bioreactor according to claim 5, further comprising a drain pipe (14), the drain pipe (14) being connected to a pipe between the water outlet (500) and the expansion tank (10) by means of an on-off valve (11).
8. The temperature-stabilized bioreactor as claimed in claim 4, characterized in that the heat exchanger (5) is connected with a first cooling water pipe (15) and a second cooling water pipe (16), an electromagnetic valve (17) is arranged on the first cooling water pipe (15), and a tuning fork level gauge (18) is arranged at the upper end of the heater (7).
9. The temperature-stabilized bioreactor according to claim 1, characterized in that the bottom of the jacket (2) is provided with a partition area (19), two chambers are arranged in the partition area (19), the two chambers are respectively communicated with a water inlet pipe (4) and a water outlet pipe (3), the water outlet (500) is communicated with the chamber connected with the water outlet pipe (3) through a water drainage groove (20), and the water inlet pipe (4) is communicated with the other chamber through a water inlet (400).
10. The temperature-stable bioreactor of claim 9, wherein a plurality of seals (200) are arranged in the jacket (2) from top to bottom, the seals (200) divide the jacket (2) into a plurality of heat exchange cavities (100), water through holes (300) are arranged on the seals (200), and the upper water through hole and the lower water through hole (300) are respectively arranged at two ends of the heat exchange cavities (100).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322033142.5U CN220579246U (en) | 2023-07-31 | 2023-07-31 | Temperature-stable bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322033142.5U CN220579246U (en) | 2023-07-31 | 2023-07-31 | Temperature-stable bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220579246U true CN220579246U (en) | 2024-03-12 |
Family
ID=90119266
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322033142.5U Active CN220579246U (en) | 2023-07-31 | 2023-07-31 | Temperature-stable bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220579246U (en) |
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2023
- 2023-07-31 CN CN202322033142.5U patent/CN220579246U/en active Active
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